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Dissertations / Theses on the topic 'Nucleic-acid Amplification and Quantification'

Author: Grafiati

Published: 6 September 2023

Last updated: 31 July 2025

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1

Hernández-Neuta, Iván. "Nucleic acid analysis tools : Novel technologies and biomedical applications." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-146334.

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Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. Therefore, accessing these sequences provides key information regarding the molecular identity and functional state of any living organism, this is very useful for areas like biomedicine, where accessing and understanding these molecular signatures is the key to develop strategie

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2

Lee, Dong-Hun. "Nucleic acid amplification testing for screening of individual blood units." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2007. http://dare.uva.nl/document/48208.

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3

Daher, Rana. "Recombinase polymerase amplification technology : Assessment for nucleic acid-based acid-based point-of-care diagnostics." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26269.

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Cette thèse de doctorat porte dans l’ensemble une étude approfondie sur une technologie émergente pour l’amplification isotherme des acides nucléiques appelée recombinase polymerase amplification (RPA). L’introduction porte une description détaillée sur la RPA. Cette revue de littérature documente et discute les diverses applications de la RPA en soulignant les connaissances actuelles concernant les applications diagnostiques. Malgré la composition complexe de la RPA (6 à 7 protéines dans le même mélange réactionnel), cette dernière s’avère une technologie rapide (générant des résultats < 2

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4

Matinyenya, Brian. "Novel and newer nucleic acid amplification tests for the diagnosis of TB." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20680.

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Background: Current tools for TB diagnosis have suboptimal accuracy, perform poorly in diagnosing extra-pulmonary TB, and are not point of care; hence results have a slow turn-around time. Objective: This project evaluated the diagnostic accuracy of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with suspected TB (the accuracy of Xpert using these fluids was unk

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5

Syed, Shahida Nina. "Electrochemical control of reversible DNA hybridisation : for future use in nucleic acid amplification." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/9617.

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Denaturation and renaturation is indispensable for the biological function of nucleic acids in many cellular processes, such as for example transcription for the synthesis of RNA and DNA replication during cell division. However, the reversible hybridisation of complementary nucleic acids is equally crucial in nearly all molecular biology technologies, ranging from nucleic acid amplification technologies, such as the polymerase chain reaction, and DNA biosensors to next generation sequencing. For nucleic acid amplification technologies, controlled DNA denaturation and renaturation is particula

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6

Thomas, Alistair Owen. "Detection of bacterial gene expression by a novel isothermic nucleic acid amplification technology." Thesis, University of Bath, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410924.

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A novel probe-based technique called Signal-Mediated Amplification Reaction Technology (SMART) was optimised for detection of RNA targets in order to quantify gene expression. The SMART assay was used to quantify both 23 S rRNA in P. aeruginosa PAOl and gfpmuti mRNA in the plasmid-borne rpoSwgfpmvXi fusions P. aeruginosa SS429 and SS431. However, the assay was not sufficiently sensitive to detect gfpmvfo mRNA from the chromosomal rpoS::gfpmut3 fusion P. aeruginosa SS336. SDS-PAGE analysis of outer membrane proteins of P. aeruginosa PAOl revealed that cells grown in a reported iron-replete chem

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7

Xiao, Linlin. "Detection of Viable Foodborne Pathogens and Spoilage Microorganisms by Nucleic Acid Amplification Based Platforms." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308284180.

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8

CORAL, LUCIA. "HIGH-RESOLUTION NUCLEIC ACID ANALYSIS WITH A DNA NANOTECHNOLOGY APPROACH." Doctoral thesis, Università degli Studi di Trieste, 2017. http://hdl.handle.net/11368/2908115.

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The goal of my research program is to develop a DNA-based nanosensor for nucleic acids analysis. I plan to use DNA Origami nanostructures that are formed by a-few-thousand-nucleotides-long, circular, single stranded (ss)DNA “scaffold” folded to form a specific shape by the action of a few hundreds of short (approx. 30 nucleotides) ssDNA “staples”, which hybridize over non-consecutive regions of the scaffold. Staples can be incorporated within the structure with well-defined stoichiometry and some of them can be designed to serve as highly-specific receptor for short nucleic acids sequences. I

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9

Choi, Kwan-yue. "A molecular epidemiology study on conjunctivitis using conventional nucleic acid amplification technologies and resequencing microarray." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B44248465.

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10

Choi, Kwan-yue, and 蔡君如. "A molecular epidemiology study on conjunctivitis using conventional nucleic acid amplification technologies and resequencing microarray." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B44248465.

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11

Bowers, Katherine. "Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis." Thesis, University of Westminster, 2017. https://westminsterresearch.westminster.ac.uk/item/q56v6/development-and-clinical-performance-of-nucleic-acid-amplification-techniques-for-the-diagnosis-of-strongyloides-stercoralis.

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The laboratory diagnosis of Strongyloides stercoralis (S. stercoralis) at the Department of Clinical Parasitology (DCP) by the routine methods of microscopy and Strongyloides culture is not sensitive due to the, usually, low parasite load and intermittent larval excretion of the parasite. Serology (enzyme-linked immunosorbent assay) suffers from a lack of specificity because Strongyloides antibodies are known to cross- react with schistosomal, filarial and other helminthic antibodies in serological tests. Moreover, antibody levels are slow to decline after successful treatment therefore serolo

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12

Henriksson, Sara. "Application of Padlock Probe Based Nucleic Acid Analysis In Situ." Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-128446.

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The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. In this thesis, in situ detection of nucleic acids with padlock probes and rolling circle amplification was applied for detection of DNA in the single cell gel electrophoresis assay to detect nuclear and mitochondrial DNA

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13

Lehnus, Massimiliano. "Bio-BCA (Bio-Barcode Cascade Amplification) : development of a photosensitive, DNA-based exponential amplification platform technology for the detection of nucleic acid biomarkers." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277915.

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14

Dann, Louise Claire. "Nucleic acid sequence-based amplification : relative performance and applications in HIV-1 disease monitoring and patient management." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272347.

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15

Grant, Paul Robert. "Development and application of nucleic acid amplification technology (NAT) for the detection of viruses in donated blood." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408734.

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16

Garneret, Pierre. "Microfluidique papier pour le diagnostic de terrain : préparation d'échantillon et multiplexage." Thesis, Paris Sciences et Lettres (ComUE), 2019. https://pastel.archives-ouvertes.fr/tel-03174261.

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Depuis l’épisode EBOLA de 2014, le laboratoire MMN et de l’Institut Pasteur collaborent pour réaliser des tests de biologie moléculaire sur papier microfluidique pour le diagnostic de maladies infectieuses. Des tests réalisés à Macenta (Guinée) sur des échantillons d’ARN purifiés de patients ont démontré la pertinence de la technologie. Aujourd’hui, les deux équipes travaillent au développement d’un dispositif multiplexé (détection simultanée de plusieurs cibles biologiques). L’émergence du Virus Zika (ZIKV) transmis majoritairement par les moustiques diurnes du genre Aedes, dans des zones géo

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17

Masetty, Manaswini. "A Smartphone Enabled Molecular Diagnostic Toolkit to Detect Pathogens via Isothermal Nucleic Acid Amplification on Pre-Dried Disposable Paper Strips." University of Cincinnati / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1627664394713446.

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18

Hofmann, Jakob [Verfasser]. "Evaluation der klinischen Praktikabilität und prognostischen Aussagefähigkeit der intraoperativen Sentinellymphknotendiagnostik mittels One-step Nucleic Acid Amplification (OSNA) bei invasivem Mammakarzinom / Jakob Hofmann." Kiel : Universitätsbibliothek Kiel, 2014. http://d-nb.info/1053326211/34.

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19

Bernander, Sverker. "Detection and epidemiologic subtyping of Legionella pneumophila using DNA-based molecular methods /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-745-2.

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20

Nicolini, Ariana Marie, and Ariana Marie Nicolini. "Single-Step, Optical Biosensors for the Rapid and Sensitive Detection of Bacterial and Viral Pathogens." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/623158.

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This dissertation discusses the development of inexpensive, easy-to-use, and field-deployable diagnostic techniques and devices for the early detection of various pathogens, commonly found in clinical samples and contaminated food and water. Infectious diseases account for about 90% of world health problems, killing approximately 14 million people annually, the majority of which reside in developing countries. In 2012, the World Health Organization (WHO) published data on the top 10 causes of death across the globe. Although communicable disease is a prevalent cause of fatality, both low-incom

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21

Faltin, Bernd [Verfasser], and Roland [Akademischer Betreuer] Zengerle. "Mediator Probe PCR: a novel assay principle for universal real-time detection of nucleic acid amplification = Mediator Probe PCR: ein neuartiger Ansatz zur universellen Echtzeit-Detektion von Nukleinsäuren." Freiburg : Universität, 2013. http://d-nb.info/1123477000/34.

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22

Hadersdorfer, Johannes [Verfasser], Dieter Richard [Akademischer Betreuer] Treutter, and Thilo [Akademischer Betreuer] Fischer. "Development of an isothermal nucleic acid amplification protocol for high-throughput monitoring of Plum pox virus infection in stone fruit production / Johannes Hadersdorfer. Gutachter: Dieter Richard Treutter ; Thilo Fischer. Betreuer: Dieter Richard Treutter." München : Universitätsbibliothek der TU München, 2013. http://d-nb.info/1032313498/34.

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23

Gomez, Deborah Beltrami. "Prevalência de Chlamydia trachomatis em mulheres inférteis e gestantes assintomáticas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/143385.

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Introdução: A infecção urogenital por Chlamydia trachomatis é a doença sexualmente transmissível bacteriana mais prevalente no mundo e afeta principalmente mulheres jovens sexualmente ativas. Infecções não tratadas podem provocar complicações reprodutivas decorrentes do dano tubáreo. Na gestação, aumenta o risco de parto prematuro, baixo peso ao nascer, morte perinatal, conjuntivite e pneumonia neonatal. Existem poucos dados brasileiros referentes à epidemiologia dessa infecção no nosso meio. O objetivo desse estudo foi determinar a prevalência de C. trachomatis em mulheres inférteis e em gest

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24

Pugnière, Pascal. "Contribution à l'amélioration de la quantification des acides nucléiques par qPCR et RT-qPCR." Phd thesis, Université de Grenoble, 2012. http://tel.archives-ouvertes.fr/tel-00870512.

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La qPCR est actuellement la technique de référence en matière de quantification d'ADN. Elle peut être définie comme une amplification exponentielle, cyclique et ciblée de la séquence d'ADN cible. Le caractère exponentiel de la qPCR est à la fois à l'origine de la sensibilité de la méthode mais aussi d'une potentielle variabilité inter-échantillons. Cette variabilité est compensée par le caractère cyclique de la méthode qui entraine une synchronisation de la réaction pour tous les échantillons à chaque cycle. L'amplification ciblée de la séquence choisie traduit quand à elle la spécificité de l

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25

Maree, Hans Jacob. "Identification and characterisation of grapevine leafroll-associated virus 3 genomic and subgenomic RNAs." Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5417.

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Thesis (PhD (Genetics))--University of Stellenbosch, 2010.<br>Includes bibliography.<br>Title page: Dept. of Genetics, Faculty of Science<br>ENGLISH ABSTRACT: Grapevine leafroll-associated virus 3 (GLRaV-3) is the type strain for the genus Ampelovirus, family Closteroviridae. There has been only one report that claimed the complete nucleotide sequence of GLRaV-3 (isolate NY-1, AF037268). Here we report the complete sequence of the South African GLRaV-3, isolate GP18 (EU259806) and show a significantly extended 5’ end. We used RLM-RACE to determine the 5’ end of GP18 and found the 5’ UTR t

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26

Gualberto, Felipe Augusto Souza. "Valor disgnóstico da nested PCR em tempo real em pacientes com meningite tuberculosa." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5134/tde-26082014-093325/.

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Introdução: A meningite tuberculosa (MTB) é a forma mais grave e fatal de tuberculose. O diagnóstico oportuno e o tratamento adequado e precoce são os principais fatores associados com o bom prognóstico. Os métodos utilizados na prática médica diária - achados clínicos, exames de imagem e análise de líquido cefalorraquidiano (LCR) - têm baixa acurácia. A pesquisa do DNA do Mycobacterium tuberculosis no LCR através da reação em cadeia da polimerase (PCR, do inglês polimerase chain reaction) com a metodologia nested é promissora, especialmente quando associada à praticidade da amplificação do DN

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27

Priyanka, V. "Droplet Isothermal Amplification For Nucleic Acid Quantification." Thesis, 2022. https://etd.iisc.ac.in/handle/2005/5643.

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Nucleic acid quantification (NAQ) is extensively employed for gene expression analysis, monitoring viral loads, detecting rare or dysfunctional cells, and assessing treatment regimes. The gold standard, quantitative polymerase chain reaction (qPCR), and the recent alternative, droplet digital PCR (ddPCR), provide accurate quantification of nucleic acids (NA). Albeit the requirement of thermal cycling and separate platforms for droplet generation, NA amplification, and signal detection, in the case of ddPCR increases the assay complexity and time, limiting its broad applicability. In this work

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28

Jue, Erik Bradley. "Improved Tools for Point-of-Care Nucleic Acid Amplification Testing." Thesis, 2020. https://thesis.library.caltech.edu/13735/1/200529_erik_jue_2020_thesis_final.pdf.

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There is a critical need for improved diagnostic tools to detect infectious diseases, especially in low-resource regions. A sample-to-answer point-of-care nucleic acid amplification test (NAAT) would be incredibly valuable for many different applications (e.g. COVID-19, Chlamydia/Gonorrhoeae, Influenza, Ebola, Zika/Chikungunya/Dengue, etc.). However, sample preparation (purification of pure nucleic acids) is a challenging bottleneck. In Chapter 2, commercial NA extraction methods were studied and improved. In Chapter 3, commercial stocks of SARS-CoV-2 RNA used in FDA emergency-use authorizatio

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29

Hsieh, Tsung-Min, and 謝宗閔. "Micro Thermocyclers for Nucleic Acid Amplification with High Thermal Uniformity." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/30145900653046174870.

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博士<br>國立成功大學<br>電機工程學系碩博士班<br>96<br>The trend of miniaturization of biomedical instruments began in the last century, and is crucial for furthering progress in medical technology. In this study, an integrated chip–PCR/RT-PCR system is implemented successfully, meeting the requirements for use in miniature thermocyclers – small in size, low power consumption, high heating and cooling rates, and even portability, with resistance temperature detectors and thin-film micro-heaters. Furthermore, due to the modifications made to the micro thermocycler, the thermal uniformity of a specific region has

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Drake, Philip, Y.-C. Chen, I. Lehmann, and P.-S. Jiang. "Nanoparticle labels for pathogen detection through nucleic acid amplification tests." 2014. http://hdl.handle.net/10454/10333.

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Yes<br>Magnetic nanoparticles and surface-enhanced Raman scattering (SERS) active nanoparticles were coated with short chain DNA tags. These were then used to identify a target bacterial DNA sequence. The tags function as primers in a standard PCR with the reverse primers and forward primers on the SERS nanoparticles and magnetic nanoparticles, respectively. During the PCR cycles, a composite nanostructure is formed that is both magnetically responsive and SERS active. After magnetic trapping, the intensity of the SERS signal can be related back to the concentration of the target DNA. A test a

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31

Ping-Hua, Teng, and 鄧秉華. "Application of isothermal nucleic acid amplification on shrimp viral disease diagnosis." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/39938024179183265321.

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博士<br>東海大學<br>畜產與生物科技學系<br>97<br>Shrimp viral diseases are important issues in aquaculture industries. Until now, there have been not efficient vaccines or therapeutic strategies against these viral diseases. To face the threats, farmers tried to decrease cultivation risks with more efficient methods. However, they had no tools to early detect and prevent viruses into cultivation environment. For the purpose of pathogen detection before disease outbreak, a diagnostic platform with efficiency and accuracy plays an important role for health management of shrimp culture. Traditional strategies, s

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32

Panwar, Jatin. "Droplet Microfluidics for Nucleic Acid Quantification and Single Cell Analysis." Thesis, 2019. https://etd.iisc.ac.in/handle/2005/5018.

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Droplet microfluidics provides controlled generation of monodispersed droplets of the order of a few picoliters in multiphase microfluidic systems. These droplets are employed as micro-reactors to conduct chemical/biochemical reactions and assays in a controlled and high-throughput manner that find applications in point-of-care and lab-on-chip platforms. While the microfluidic devices are compact, the existing solutions to control fluid flow operations have a significant footprint that effects their portability, logistic viability and economics. As an alternate to the existing instrumentation-

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33

Yang, Litao. "Coupling aptamer biosensors to signal amplification." Thesis, 2007. http://hdl.handle.net/2152/3098.

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Yang, Litao 1976. "Coupling aptamer biosensors to signal amplification." 2007. http://hdl.handle.net/2152/13284.

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35

Liao, Chia-Sheng, and 廖家陞. "Automatic Nucleic Acid Amplification Microsystems for Rapid Pathogen Diagnosis of Infectious Disease." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/69312723014061329434.

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碩士<br>國立成功大學<br>微機電系統工程研究所<br>93<br>Rapid advances in biochemistry and bio-genetics lead to molecular biology, which is revolutionizing scientific thought. A micro-electro-mechanical-system (MEMS) allows human manipulation of micrometer-scale molecules. Advancement in biotechnology will hopefully improve the quality of human life. 　This investigation presents an automatic rapid diagnosis microsystem based on nucleic acid amplification. The miniature system is fabricated using MEMS techniques, and consists of a micro temperature control module and a microfluidic control module. The heating and

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Chou, Wen-Pin, and 周文彬. "Development of a Novel Nucleic Acid Amplification System-Capillary Convective Polymerase Chain Reaction." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/19692785520165047927.

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博士<br>國立臺灣大學<br>機械工程學研究所<br>99<br>A typical polymerase chain reaction (PCR) is a process containing three temperature steps for denaturation, annealing and elongation. In a traditional thermal cycler, metal block is used to heat or cool the reaction tubes, and more than two hours are needed for 45 cycles of the repeat heating and cooling steps. However, expensive thermal controller and long time-consuming made thermal cyclers are not suitable in self-test for personal use. In this study we describe a new method for DNA amplification using a principle of convection. In this platform, only a dry

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Loan, Thomas. "Cell Lysate as a Platform Technology for Biocatalytic Synthesis and Nucleic Acid Amplification." Phd thesis, 2020. http://hdl.handle.net/1885/204831.

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The research presented in this Thesis focuses on the enzymatic synthesis of value-added products using cell lysates prepared from E. coli as low-cost biocatalysts. Naturally abundant endogenous enzymes are leveraged where possible and these are augmented with heterologous proteins, incorporated by recombinant overexpression, to create multi-enzymatic cascade reactions. A robust ATP regeneration system was developed, utilizing the endogenous acetate kinase (Ack) with acetyl phosphate. Application to biocatalytic synthesis of UTP from uridine was demonstrated by incorporation of a recombinant

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DAI, NING, and 戴寧. "Development of Thermostatic Nucleic Acid Amplification Technology for Detecting Acute Hepatopancreatic Necrosis Disease (AHPND)." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/06651700425244830036.

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碩士<br>國立高雄海洋科技大學<br>海洋生物技術研究所<br>104<br>The outbreak of acute hepatopancreatic necrosis disease (AHPND) from 2009 has caused mass losses in shrimp farms worldwide. The strain of Vibrio parahaemolyticus which obtain a plasmid and secret toxins has been determined as the infectious agent. PCR methods were published and used to perform diagnosis in laboratory. This study developed methods of Loop-mediated isothermal amplification (LAMP) and Recombinase polymerase amplification (RPA) to detect AHPND and aimed to carry out the diagnosis at the farm side. Compared to PCR, the nucleic acid amplificat

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Lien, Kang-Yi, and 連剛逸. "Miniature Microfluidic System Integrated with a Sample Pretreatment Device for Rapid Nucleic Acid Amplification." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/84844620753199418940.

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碩士<br>國立成功大學<br>奈米科技暨微系統工程研究所<br>95<br>Recently, purification and enrichment of bio-samples is crucial for the analysis of biosamples with an ultra-low concentration that the performance of the detection system can be efficiently increased. Apart from that, clinical samples usually contain biological medium that would normally inhibit the subsequent ribonucleic acid (RNA)/deoxyribonucleic acid (DNA) amplification process. Moreover, there still need several tedious purification and washing steps and labor-intensive processes to complete the sample preparation. In addition, a number of large-sca

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Chen, Wei-Jang, and 陳位彰. "The Design of Temperature Control Mechanism and Its Function on Nucleic Acid Quantification." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/fj79hp.

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碩士<br>國立臺北科技大學<br>能源與冷凍空調工程系碩士班<br>96<br>This Real Time PCR machine allows the detection of DNA amplification through out the detection of the fluorescence labeling dye in the PCR mix during the early phase of this reaction. In addition, the concentration of target DNA fragment in the PCR mix before thermal cycling can be obtained from the time recorded history of the fluorescence intensity by integrating thermal cycler and fluorimeter. The Real-Time PCR machine has higher sensitivity and consumes less time than those of the traditional PCR machine. This study modified the temperature control

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Chen, Lin [Verfasser]. "Miniaturised nucleic acid analysis systems : purification, amplification and real-time detection / vorgelegt von Lin Chen." 2007. http://d-nb.info/997471891/34.

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Huang, Fu-Chun, and 黃富駿. "An Integrated Microfluidic System for Nucleic Acid Amplification, Electrophoresis Separation and On-line Optical Detection." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/38805165998727799529.

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博士<br>國立成功大學<br>工程科學系碩博士班<br>96<br>This dissertation presents an integrated microfluidic chip capable of performing DNA/RNA (Deoxyribonucleic acid/Ribonucleic acid) amplification, electrokinetic sample injection and separation, and on-line optical detection of polymerase chain reaction (PCR) products in an automatic mode. In the device introduced here, DNA/RNA samples are first replicated using a micromachine-based PCR module or reverse transcription polymerase chain reaction (RT-PCR) module and then transported by a pneumatic micropump to a sample reservoir. The samples are subsequently drive

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Wang, Tzu-Chia, and 王子嘉. "Integrated Nucleic Acid Extraction, Amplification, and Detection on Paperfluidics for Diagnostics of Orchid Virus Disease." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/sk5w8v.

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碩士<br>國立臺灣大學<br>機械工程學研究所<br>107<br>In the study, we combined the extraction, amplification, and detection of nucleic acids in molecular diagnostics, and integrated them into paper-based microfluidics (paperfluidics) which was low-cost, lightweight, and portable. Paperfluidics reduced the complicated process and operation time compared to traditional molecular diagnostic technology and did not require precise instruments. This study developed point of care (POC) device that combined known technology and innovational methods, which could diagnose two major orchid viruses. First, the results show

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Huang, Hsuan-Shian, and 黃宣憲. "Nucleic acid detection, quantification and sequence analysis of canine distemper virus from clinical specimens." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/95670168567838042013.

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碩士<br>國立臺灣大學<br>獸醫學研究所<br>93<br>Canine distemper virus (CDV) infection in dogs can result in subclinical infection, gastrointestinal signs, and/or respiratory signs, frequently with central nervous system (CNS) involvement, high morbidity and mortality. Recently, the incidence of canine distemper (CD) both in unvaccinated and vaccinated dogs seemed increasing in Taiwan. In order to understand more about the current CDV infection in Taiwan, a rapid and sensitive diagnotic test for CD using a RT-PCR combined with nested PCR was applied to 440 dogs clinically suspected with CDV infection. 660 cli

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Ho, Ya-Lun, and 何亞倫. "Development of a Surface Acoustic Wave Based Micro-Droplet Control System and its Application of Nucleic Acid Amplification." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/43732841823831743871.

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碩士<br>國立臺灣大學<br>應用力學研究所<br>99<br>In this thesis, an automatic micro-droplet control system applied to amplification of nucleic acid is accomplished by the combination of a surface acoustic wave (SAW) device, micro-heaters, micro-sensors of temperature, and a PI controller. The SAW device constituted of slanted finger interdigital transducers (SFITs) is utilized to actuate the micro-droplet by the acoustic streaming and to detect the micro-droplet by the frequency responses of the SAWs. With the development of PI controller, the micro-droplet can be manipulated automatically. In order to reduce

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Chen, Jiann-Mou, and 陳建謀. "A Rapid and Real-time Nucleic Acid Detection System by Fluorescence Resonance Energy Transfer and Isothermal RNA Amplification." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/28118597521477734866.

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碩士<br>國立臺灣大學<br>電機工程學研究所<br>94<br>In this thesis, we develop a detecting method with high accuracy and high sensitivity for specific RNA sequences in isothermal environment. We construct a low-cost and real-time system for detection specific RNA sequences by using this method. Isothermal RNA amplification is a powerful tool to amplify specific sequences of RNA. It can successfully amplify as low as 1 ng of total RNA in 90 minutes. Therefore, we integrate isothermal RNA amplification and Fluorescence Resonance Energy Transfer (FRET) methods to real-time detect fluorescent signals for specif

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Goodrich, Terry T. "Ultrasensitive nucleic acid detection using surface plasmon resonance imaging measurements via a novel surface amplification process and microfluidic networks." 2004. http://www.library.wisc.edu/databases/connect/dissertations.html.

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Cox, Philipp [Verfasser]. "Etablierung und Evaluation der NASBA (Nucleic-acid-sequence-based-amplification)-Technologie zum Nachweis von Aspergillus-RNA / vorgelegt von Philipp Cox." 2006. http://d-nb.info/97814404X/34.

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Kosťun, Jan. "Využití molekulárně biologické metody One-Step Nucleic Acid Amplification (OSNA) při vyšetření sentinelových lymfatických uzlin u pacientek s karcinomem endometria." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-391393.

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Hypothesis The One-Step Nucleic Acid Amplification method could represent an effective intraoperative tool for detection of metastatic involvement of lymphatic nodes on the level of ultrastaging in endometrial cancer patients. Objective Utilization of the One-Step Nucleic Acid Amplification (OSNA) molecular biology method for the detection of the micrometastatic and macrometastatic involvement of sentinel lymph nodes in endometrial cancer patients. The objective is a comparison with the conclusion of the histopathological ultrastaging of sentinel lymph nodes and a description of the clinical c

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Omar, S. V. (Shaheed Vally). "Innovative approaches to tuberculosis diagnosis with emphasis on nucleic acid amplification tests in a resource constrained high burden tuberculosis setting." Thesis, 2015. http://hdl.handle.net/2263/50754.

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The global control of tuberculosis (TB) is currently hindered by the low sensitivity of microscopy and the prolonged time-to-result of culture. Recent technical progress has improved both diagnostic accuracy and turnaround, namely, nucleic acid amplification tests (NAAT). The World Health Organization (WHO) has recently endorsed two NAATs, which South Africa has been in the forefront of adopting. Based on WHO recommendations, the Xpert MTB/RIF assay (Xpert) has replaced microscopy as the firstline test in the National Algorithm. With current research and development primarily focused on

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