Relevant bibliographies by topics / Nucleic acid components

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Nucleic acid components'

Author: Grafiati
Published: 9 March 2023

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Journal articles on the topic "Nucleic acid components"

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Bergen, Jamie M., and Suzie H. Pun. "Peptide-Enhanced Nucleic Acid Delivery." MRS Bulletin 30, no. 9 (2005): 663–67. http://dx.doi.org/10.1557/mrs2005.194.

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AbstractNumerous barriers, both extracellular and intracellular, hinder successful and efficient nonviral nucleic acid delivery. Due to their small size and ability to specifically recognize and interact with molecular targets, peptides can be incorporated as modular elements into synthetic nucleic acid delivery systems to overcome many of these barriers. Three classes of peptides that have frequently been integrated as components in nucleic acid delivery systems include cell-penetrating peptides (CPPs), endosomal release peptides, and nuclear localization sequences (NLSs).Various additional classes of peptides show promise for enhancing nucleic acid delivery by targeting cell surface receptors, inhibiting nuclease activity, and directing nucleic acids toward intracellular targets. In addition to a review of the various existing approaches to peptide-enhanced nucleic acid delivery, this article will discuss strategies for the development of new peptides and approaches for the incorporation of these peptides into nucleic acid delivery systems.
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Lesk, V. I., and A. M. Lesk. "Schematic diagrams of nucleic acids and protein–nucleic acid complexes." Journal of Applied Crystallography 22, no. 6 (1989): 569–71. http://dx.doi.org/10.1107/s0021889889008265.

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Simplified representations of components of nucleic acids have been designed and implemented as programs integrated with other software that draws schematic diagrams of proteins. Examples illustrating the structures of oligonucleotides, tRNA and a protein–nucleic acid complex indicate the utility of these representations for making intelligible illustrations of complex structures containing nucleic acids.
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Alexander, Petr, and Antonín Holý. "Prodrugs of Analogs of Nucleic Acid Components." Collection of Czechoslovak Chemical Communications 59, no. 10 (1994): 2127–65. http://dx.doi.org/10.1135/cccc19942127.

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This review regards various attitudes to the rational design of prodrugs derived of nucleoside and nucleotide analogues with pronounced biological (antiviral, anticancer) activity. Particular attention is focused on the recent development of prodrugs of approved therapeutic agents with the above structural association.
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Dickerson, R. E. "Definitions and nomenclature of nucleic acid structure components." Nucleic Acids Research 17, no. 5 (1989): 1797–803. http://dx.doi.org/10.1093/nar/17.5.1797.

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Dračínský, Martin, and Paul Hodgkinson. "Solid-state NMR studies of nucleic acid components." RSC Advances 5, no. 16 (2015): 12300–12310. http://dx.doi.org/10.1039/c4ra14404j.

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Gheerardijn, Vicky, and Annemieke Madder. "XVIth Symposium on Chemistry of Nucleic Acid Components." ChemBioChem 15, no. 17 (2014): 2495–98. http://dx.doi.org/10.1002/cbic.201402460.

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Rubin, Yu V., and L. F. Belous. "Molecular Structure and Interactions of Nucleic Acid Components in Nanoparticles: Ab Initio Calculations." Ukrainian Journal of Physics 57, no. 7 (2012): 723. http://dx.doi.org/10.15407/ujpe57.7.723.

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Self-associates of nucleic acid components (stacking trimers and tetramers of the base pairs of nucleic acids) and short fragments of nucleic acids are nanoparticles (linear sizes of these particles are more than 10 Å). Modern quantum-mechanical methods and softwares allow one to perform ab initio calculations of the systems consisting of 150–200 atoms with enough large basis sets (for example, 6-31G*). The aim of this work is to reveal the peculiarities of molecular and electronic structures, as well as the energy features of nanoparticles of nucleic acid components.
 We had carried out ab initio calculations of the molecular structure and interactions in the stacking dimer, trimer, and tetramer of nucleic base pairs and in the stacking (TpG)(ApC) dimer and (TpGpC) (ApCpG) trimer of nucleotides, which are small DNA fragments.
 The performed calculations of molecular structures of dimers and trimers of nucleotide pairs showed that the interplanar distance in the structures studied is equal to 3.2 Å on average, and the helical angle in a trimer is approximately equal to 30º. The distance between phosphor atoms in neighboring chains is 13.1 Å. For dimers and trimers under study, we calculated the horizontal interaction energies.
 The analysis of interplanar distances and angles between nucleic bases and their pairs in the calculated short oligomers of nucleic acid base pairs (stacking dimer, trimer, and tetramer) has been carried out. Studies of interactions in the calculated short oligomers showed a considerable role of the cross interaction in the stabilization of the structures. The contribution of cross interactions to the horizontal interactions grows with the length of an oligomer. Nanoparticle components get electric charges in nanoparticles. Longwave low-intensity bands can appear in the electron spectra of nanoparticles.
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Christel, L. A., K. Petersen, W. McMillan, and M. A. Northrup. "Rapid, Automated Nucleic Acid Probe Assays Using Silicon Microstructures for Nucleic Acid Concentration." Journal of Biomechanical Engineering 121, no. 1 (1999): 22–27. http://dx.doi.org/10.1115/1.2798037.

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A system for rapid point-of-use nucleic acid (NA) analysis based on PCR techniques is described. The extraction and concentration of DNA from test samples has been accomplished utilizing silicon fluidic microchips with high surface-area-to-volume ratios. Short (500 bp) and medium size (48,000 bp) DNA have been captured, washed, and eluted using the silicon dioxide surfaces of these chips. Chaotropic (GuHCl) salt solutions were used as binding agents. Wash and elution agents consisted of ethanol-based solutions and water, respectively. DNA quantities approaching 40 ng/cm2 of binding area were captured from input solutions in the 100–1000 ng/mL concentration range. For dilute samples of interest for pathogen detection, PCR and gel electrophoresis were used to demonstrate extraction efficiencies of about 50 percent, and concentration factors of about 10× using bacteriophage lambda DNA as the target. Rapid, multichannel PCR thermal cycling modules with integrated solid-state detection components have also been demonstrated. These results confirm the viability of utilizing these components as elements of a compact, disposable cartridge system for the detection of NA in applications such as clinical diagnostics, biowarfare agent detection, food quality control, and environmental monitoring.
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Huynh-Dinh, T. "Seventh symposium on the chemistry of nucleic acid components, nucleic acids symposium series no. 18." Biochimie 70, no. 2 (1988): 299. http://dx.doi.org/10.1016/0300-9084(88)90079-x.

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Panigaj, Martin, M. Brittany Johnson, Weina Ke, et al. "Aptamers as Modular Components of Therapeutic Nucleic Acid Nanotechnology." ACS Nano 13, no. 11 (2019): 12301–21. http://dx.doi.org/10.1021/acsnano.9b06522.

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Dissertations / Theses on the topic "Nucleic acid components"

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Park, Tae Kyo. "Molecular recognition of nucleic acid components." Thesis, Massachusetts Institute of Technology, 1992. http://hdl.handle.net/1721.1/13113.

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Evans, Delwyn Roger. "Reactions of ruthenium complexes with nucleic acid components." Thesis, University of York, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306345.

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Fitchett, M. "E.S.R. and pulse radiolysis studies of the radical reactions of nucleic acid components." Thesis, University of York, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356166.

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Boyle, Bryan. "The structural chemistry of fluorinated nucleic acid components and possible implications for fluorinated DNA self-assembly." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/3198/.

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The main focus of this project has been the investigations into the effect that fluorine substitution has on structures of molecular complexes containing DNA base molecules. Previous work done on these molecules has been targeted at producing anti-cancer or antiviral treatments, which reflects an important ultimate aim of investigations in this area. Success in this structural project would make a contribution towards this aim, by helping the understanding of the effects of fluorination on some of the interactions that control DNA self-assembly, notably base-pairing and stacking interactions, together with additional interactions involving fluorine. The specific aim of this project was to grow crystals of molecular complexes of cytosine and 5-fluorocytosine with co-molecules and use the structural descriptions to assess the difference between the structures and the influence the presence of the fluorine atom has on the structure when compared to the nonfluorinated equivalent. Single crystal X-ray diffraction has been the main method of analysis, backed up by related techniques. Many crystallisations have been set up with cytosine and 5-fluorocytosine with a wide range of targeted co-molecules. 5-fluorouracil and uracil have also been used in related co-crystallisations with the aim of producing related complexes of these materials with the same co-molecules as used with cytosine and 5-fluorocytosine. In both families of complexes it was hoped that the fluorine would have an effect on the base-pairing motifs adopted in the structures, with the control being the non-fluorinated structure. The program dSNAP has been used to give a comparison of the structures produced in this work with those already reported in the CSD. This program has given an indication of the classification of the standard primary bonding motifs and to analyse the other features commonly seen in base pairs such as buckling and propeller twisting. The comprehensive series of complexes produced have provided the opportunity to examine significant structural trends, notable in the adoption of various base-pair motifs based on the Watson-Crick, Hoogsten and derived base-pairing patterns. The degree of proton transfer to the ring nitrogen of the cytosine could be rationalised in terms of the DpKa values, and the proton transfer has a substantial effect on the base-pair motifs able to be adopted. The classification of the complex structures in terms of hetero (pseudo)-basepairing has also been found to be of value. Extensive base-stacking and weaker interactions involving fluorine are also present; these have been analysed and found to have significant effects on the structures adopted, and hence may have future implications for the assembly of fluorinated DNA.
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Albalawi, Karma. "A double competition dialysis assay for the analysis of the distribution of optoelectronically active components over nucleic acid structures." Thesis, Cardiff University, 2018. http://orca.cf.ac.uk/119448/.

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This thesis presents DNA binding studies and our work to develop a double competition dialysis assay. Chapter 1 describes DNA structure, including duplex, triplex and quadruplex structures, and functioning in storing the genetic code. This Chapter also presents an overview of the interactions of small molecules with nucleic acids structures. Moreover, the chapter describes the techniques that have been used for our DNA-binding studies, viz, UV - visible spectroscopy, circular dichroism spectroscopy and isothermal titration calorimetry. The chapter also describes potential applictions of small molecule DNA binders. Finally, we describe the competition dialysis in this chapter. Chapter 2 describes the determination of extinction coefficients for selected optoelectronically active π-conjugated molecules in aqueous buffers. Furthermore, we established the light sensitivity of the compounds. In addition, the chapter describes the binding studies of nucleic acid binders from a library of available ligands using UV-visible, circular dichroism, and isothermal titration calorimetry. Chapter 3 describes the development of a custom competition dialysis device. We test this device to determine affinity and selectivity of ligands for nucleic acids structures. We analysed the affinity and selectivity of a single ligand for FS-DNA, specific duplex sequences (dAdT)12●(dAdT)12 and (dGdC)12●(dGdC)12, and different quadruplex structures such as cmyc, 22AG and EAD2. The data agree with the results from UV-vis titrations. In Chapter 4 we explore how double competition dialysis allows screening of two ligands against an array of nucleic acids structures. Several compounds were tested showing that our assay deals reasonably well with fading unless the latter progresses to the extent when absorbance is too low to measure reliably. Although we have identified compounds with promising affinity profiles, even in the presence of a second binder we are yet to identify binders with an orthogonal selectivity profile. In Chapter 5 we present general conclusions and suggestions for future work.
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Pecourt, Jean-Marc L. "Femtosecond pump-probe investigation of excited state dynamics in liquids : intramolecular charge transfer in dimethylaminobenzonitrile and ultrafast internal conversion in nucleic acid components /." The Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488203857248598.

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Avvakumova, S. "GOLD NANOCONJUGATES: PREPARATION, CHARACTERISATION AND BIOLOGICAL APPLICATIONS." Doctoral thesis, Università degli Studi di Milano, 2013. http://hdl.handle.net/2434/214975.

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This PhD thesis is dedicated to the preparation, characterisation and biological application of gold nanoconjugates. Gold nanoparticles are prepared by various modern preparation methods, and subsequently characterised by spectroscopic (UV-vis, ATR-FTIR, NMR), microscopic (TEM) techniques. The stability of the conjugates is evaluated both by using Zeta-potential studies and UV-vis. The nanoparticles are used in cellular uptake experiments using human glioblastoma cancer cells, and are found to possess a low cytotoxicity. The nanoparticles are found taken up by the cells and distributed in different cellular compartments.
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Suwandi, Ronald Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "The applications of multi-component nucleic acid enzymes (MNAzymes)." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2009. http://handle.unsw.edu.au/1959.4/41307.

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The emergence of MNAzymes (Multi-component nucleic acid enzymes) provides a new approach for detection of target analytes in various applications. In this thesis, three novel MNAzyme-based methodologies were developed to expand the range of the applications of MNAzymes. MNAzymes can be coupled with DNA or RNA ligands called aptamers to generate an apta-MNAzyme system, which can be used for the detection of non-nucleic target analytes such as small molecules and proteins. Direct detection using apta-MNAzyme system is performed in a format, which was isothermal, fluorescent, rapid, and requires no protein enzymes. Apta-MNAzymes can be coupled with a signal amplification cascade to increase the sensitivity of the reaction. Another MNAzyme-based methodology termed truncated MNAzyme arm system was developed to discriminate the presence of a single base mismatch of two closely related sequences. The system employs a partzyme with a truncated sensor arm and a stabiliser oligonucleotide that binds adjacently to the truncated sensor arm to stabilise the active MNAzyme structure. Truncated MNAzyme real-time PCR system is capable of discriminating the presence of a single base mismatch in a target DNA with high specificity and sensitivity (down to approximately 10 gene copies). The generic nature of the system enables simultaneous detection of three SNP targets in a multiplex format. MNAzymes was also investigated with various strategies to discriminate DNA sequences that are either methylated or unmethylated. In this thesis, bisulphite-treated DNA samples present in as low as 0.032 % of methylated DNA in a background of unmethylated DNA were discriminated using MNAzyme real-time methylation specific PCR (MSP) system. Furthermore, the presence of 5-methylcytosines in a target sequence increases the melting temperature of the duplex DNA. This was exploited further to directly discriminate DNA methylation status of target sequences using the truncated MNAzyme arm system without the need for bisulphite modification. Findings in this thesis have broadened the scope of MNAzymes as versatile tools for many possible applications and flexible alternative to the current technologies.
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Mokany, Elisa Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "The development of multi-component nucleic acid enzymes(MNAzymes)for the detection of analytes." Awarded by:University of New South Wales, 2007. http://handle.unsw.edu.au/1959.4/35210.

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Chi, Xiuling. "BIOSYNTHETIC PATHWAY OF THE AMINORIBOSYL COMPONENT OF LIPOPEPTIDYL NUCLEOSIDE ANTIBIOTICS." UKnowledge, 2013. http://uknowledge.uky.edu/pharmacy_etds/23.

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Several lipopeptidyl nucleoside antibiotics that inhibit bacterial translocase I (MraY) involved in peptidoglycan cell wall biosynthesis contain an aminoribosyl moiety, an unusual sugar appendage in natural products. A-90289 and muraminomicin are the two representative antibiotics that belong to this family. Bioinformatic analysis of the biosynthetic A-90289 gene clusters revealed that five enzymes are likely involved in the assembly and attachment of the aminoribosyl unit. These enzymes of A-90289 are functionally assigned by in vitro characterization. The results reveal a unique ribosylation pathway that highlighted by uridine-5′-monophosphate as the source of the sugar, a phosphorylase strategy to generate a sugar-1-phosphate, and a primary amine-requiring nucleotidylyltransferase that generates the NDP-sugar donor. Muraminomicin, which has a structure similar to A-90289, holds the distinction in that both ribose units are 2-deoxy sugars. The biosynthetic gene cluster of muraminomicin has been identified, cloned and sequenced, and bioinformatic analysis revealed a minimum of 24 open reading frames putatively involved in the biosynthesis, resistance, and regulation of muraminomicin. Similar to the A-90289 pathway, fives enzymes are still likely involved in the assembly of the 2,5-dideoxy-5-aminoribose saccharide unit, and two are now functionally assigned and characterized: Mra20, a 5′-amino-2′,5′-dideoxyuridine phosphorylase and Mra23, a UTP:5-amino-2,5-dideoxy-α-D-ribose-1-phosphate uridylyltransferase. The cumulative results are consistent with the incorporation of the ribosyl appendage of muraminomicin via the archetypical sugar biosynthetic pathway that parallels A-90289 biosynthesis
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Books on the topic "Nucleic acid components"

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Symposium, on the Chemistry of Nucleic Acid Components (7th 1987 Bechyně Castle Czechoslovakia). Seventh Symposium on the Chemistry of Nucleic Acid Components: Held at Bechyně Castle, Czechoslovakia, August 30th-September 5th, 1987. IRL Press, 1987.

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The components of life: From nucleic acids to carbohydrates. Britannica Educational Pub. in association with Rosen Educational Services, 2011.

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Analogues of Nucleic Acid Components: Mechanisms of Action. Springer-Verlag, 2012.

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Seventh Symposium on the Chemistry of Nucleic Acid Components (Nucleic Acids Symposium Series, No 18). Irl Pr, 1987.

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Duchesne, J. Electrical, Optical and Magnetic Properties of Nucleic Acid and Components. Elsevier Science & Technology Books, 2012.

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Fu, Yingxian. Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components. 1993.

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Fu, Yingxian. Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components. 1993.

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Components of Life: From Nucleic Acids to Carbohydrates. Rosen Publishing Group, 2011.

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Czajkowski, C. J. Survey of boric acid corrosion of carbon steel components in nuclear plants. For sale by the Supt. of Docs., U.S. G.P.O, 1990.

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Murer, Heini, Jürg Biber, and Carsten A. Wagner. Phosphate homeostasis. Edited by Robert Unwin. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199592548.003.0025.

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Inorganic phosphate ions (H2PO4−/ HPO42−) (abbreviated as Pi) are involved in formation of bone and generation of high-energy bonds (e.g. ATP), metabolic pathways, and regulation of cellular functions. In addition, Pi is a component of biological membranes and nucleic acids. Only about 1% of total body Pi content is present in extracellular fluids, at a plasma concentration in adults within the range 0.8–1.4 mMol/L (at pH 7.4 mostly as HPO42−), with diurnal variations of approximately 0.2 mM. A small amount of plasma Pi is bound to proteins or forms complexes with calcium. Under normal, balanced conditions, absorption of dietary Pi along the small intestine equals the output of Pi via kidney and faeces. Renal excretion of Pi represents the key determinant for the adjustment of normal Pi plasma concentrations. Renal reabsorption of Pi occurs along the proximal tubules by sodium-dependent Pi cotransporters that are strictly localized at the apical brush border membrane. Parathyroid hormone (PTH) and FGF23 are key regulators amongst a myriad of factors controlling excretion of Pi in urine, mostly by changes of the apical abundance of Na/Pi cotransporters. Hypophosphataemia may result in osteomalacia, rickets, muscle weakness, and haemolysis. Hyperphosphataemia can lead to hyperparathyroidism and severe calcifications in different tissues.
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Book chapters on the topic "Nucleic acid components"

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Schram, Karl H. "Mass Spectrometry of Nucleic Acid Components." In Methods of Biochemical Analysis. John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110553.ch4.

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Hawkins, Clare L., David I. Pattison, Matthew Whiteman, and Michael J. Davies. "Chlorination and Nitration of DNA and Nucleic Acid Components." In Oxidative Damage to Nucleic Acids. Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-72974-9_2.

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Robertson, Michael, Jay Hesselberth, J. Cox, and Andrew Ellington. "Designing and selecting components for nucleic acid computers." In DNA Based Computers V. American Mathematical Society, 2000. http://dx.doi.org/10.1090/dimacs/054/15.

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Spielvogel, B. F., A. Sood, J. Tomasz, et al. "Boronated Peptides and Nucleic Acid Components for Nct." In Advances in Neutron Capture Therapy. Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2978-1_75.

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Feitelson, Mark. "Nucleic Acid Sequencing of Dane Particle DNA and Expression of HBV Genes." In Molecular Components of Hepatitis B Virus. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2573-4_12.

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Giese, Bernd, and Don McNaughton. "Raman and surface-enhanced Raman spectroscopic study of nucleic acid components." In Spectroscopy of Biological Molecules: New Directions. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_111.

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Feitelson, Mark. "The Protein Kinase Activity in HBcAg and the Proposed Nucleic Acid Binding Properties of Core Polypeptide." In Molecular Components of Hepatitis B Virus. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2573-4_8.

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Buntru, Matthias, Simon Vogel, Ricarda Finnern, and Stefan Schillberg. "Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins." In Recombinant Proteins in Plants. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2241-4_8.

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AbstractPlant cell-free lysates contain all the cellular components of the protein biosynthesis machinery, providing an alternative to intact plant cells, tissues, and whole plants for the production of recombinant proteins. Cell-free lysates achieve rapid protein production (within hours or days) and allow the synthesis of proteins that are cytotoxic or unstable in living cells. The open nature of cell-free lysates and their homogeneous and reproducible performance is ideal for protein production, especially for screening applications, allowing the direct addition of nucleic acid templates encoding proteins of interest, as well as other components such as enzyme substrates, chaperones, artificial amino acids, or labeling molecules. Here we describe procedures for the production of recombinant proteins in the ALiCE (Almost Living Cell-free Expression) system, a lysate derived from tobacco cell suspension cultures that can be used to manufacture protein products for molecular and biochemical analysis as well as applications in the pharmaceutical industry.
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Corash, L. M. "Inactivation of Viruses, Bacteria, Protozoa, and Leukocytes in Labile Blood Components by Using Nucleic Acid Targeted Methods." In Transfusion Medicine: Quo Vadis? What Has Been Achieved, What Is to Be Expected. Springer US, 2001. http://dx.doi.org/10.1007/978-1-4615-1735-1_10.

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Chmelová, K., J. Štěpánek, P. Y. Turpin, and J. Zachová. "Low-symmetry molecular single crystal as a model for vibrational study of intermolecular interactions of nucleic acid components and its analogues." In Spectroscopy of Biological Molecules: New Directions. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_310.

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Conference papers on the topic "Nucleic acid components"

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Robins, Morris J. "Transformation chemistry with nucleic acid purines." In XIVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2008. http://dx.doi.org/10.1135/css200810028.

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Campbell, Meghan A., and Jesper Wengel. "Exploring unique properties of unlocked nucleic acid." In XVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201112018.

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Kovačková, Soňa, Martin Dračínský, and Dominik Rejman. "Piperidine nucleoside phosphonic acid derivatives." In XVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201112372.

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Bethge, Lucas, Sven Hainke, Lars Röglin, Elke Socher, Stefanie Thurley, and Oliver Seitz. "Recognizing and controlling biomolecular interactions with nucleic acids." In XIVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2008. http://dx.doi.org/10.1135/css200810112.

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Dohno, Chikara, Shin-nosuke Uno, and Kazuhiko Nakatani. "Photoswitchable molecular glue for hybridization of nucleic acids." In XVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201112320.

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Balintová, Jana, Marie Brázdová, Luděk Havran, Miroslav Fojta, and Michal Hocek. "Redox labeling and redox coding of nucleic acids." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414229.

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Van Aerschot, Arthur, Mark Vandermeeren, Johan Geysen, et al. "In vitro evaluation of hexitol nucleic acid antisense oligonucleotides." In XIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 1999. http://dx.doi.org/10.1135/css199902151.

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Teulade-Fichou, Marie-Paule, Markus Kaiser, Matthieu Sainlos, et al. "Customized fused aromatics for structural recognition of nucleic acids." In XIIIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005. http://dx.doi.org/10.1135/css200507245.

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Stevens, Kristof, Marieke Op de Beeck, Sara Figaroli, and Annemieke Madder. "Synthesis and cross-linking of new furan nucleic acids." In XIVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2008. http://dx.doi.org/10.1135/css200810210.

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Gheerardijn, V., D. Buyst, J. Van Den Begin, T. Morii, J. C. Martins, and A. Madder. "Functional nucleic acids as tools in catalysis: an overview." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414267.

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Reports on the topic "Nucleic acid components"

1

Epel, Bernard L., Roger N. Beachy, A. Katz, et al. Isolation and Characterization of Plasmodesmata Components by Association with Tobacco Mosaic Virus Movement Proteins Fused with the Green Fluorescent Protein from Aequorea victoria. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7573996.bard.

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Abstract:
The coordination and regulation of growth and development in multicellular organisms is dependent, in part, on the controlled short and long-distance transport of signaling molecule: In plants, symplastic communication is provided by trans-wall co-axial membranous tunnels termed plasmodesmata (Pd). Plant viruses spread cell-to-cell by altering Pd. This movement scenario necessitates a targeting mechanism that delivers the virus to a Pd and a transport mechanism to move the virion or viral nucleic acid through the Pd channel. The identity of host proteins with which MP interacts, the mechanism of the targeting of the MP to the Pd and biochemical information on how Pd are alter are questions which have been dealt with during this BARD project. The research objectives of the two labs were to continue their biochemical, cellular and molecular studies of Pd composition and function by employing infectious modified clones of TMV in which MP is fused with GFP. We examined Pd composition, and studied the intra- and intercellular targeting mechanism of MP during the infection cycle. Most of the goals we set for ourselves were met. The Israeli PI and collaborators (Oparka et al., 1999) demonstrated that Pd permeability is under developmental control, that Pd in sink tissues indiscriminately traffic proteins of sizes of up to 50 kDa and that during the sink to source transition there is a substantial decrease in Pd permeability. It was shown that companion cells in source phloem tissue export proteins which traffic in phloem and which unload in sink tissue and move cell to cell. The TAU group employing MP:GFP as a fluorescence probe for optimized the procedure for Pd isolation. At least two proteins kinases found to be associated with Pd isolated from source leaves of N. benthamiana, one being a calcium dependent protein kinase. A number of proteins were microsequenced and identified. Polyclonal antibodies were generated against proteins in a purified Pd fraction. A T-7 phage display library was created and used to "biopan" for Pd genes using these antibodies. Selected isolates are being sequenced. The TAU group also examined whether the subcellular targeting of MP:GFP was dependent on processes that occurred only in the presence of the virus or whether targeting was a property indigenous to MP. Mutant non-functional movement proteins were also employed to study partial reactions. Subcellular targeting and movement were shown to be properties indigenous to MP and that these processes do not require other viral elements. The data also suggest post-translational modification of MP is required before the MP can move cell to cell. The USA group monitored the development of the infection and local movement of TMV in N. benthamiana, using viral constructs expressing GFP either fused to the MP of TMV or expressing GFP as a free protein. The fusion protein and/or the free GFP were expressed from either the movement protein subgenomic promoter or from the subgenomic promoter of the coat protein. Observations supported the hypothesis that expression from the cp sgp is regulated differently than expression from the mp sgp (Szecsi et al., 1999). Using immunocytochemistry and electron microscopy, it was determined that paired wall-appressed bodies behind the leading edge of the fluorescent ring induced by TMV-(mp)-MP:GFP contain MP:GFP and the viral replicase. These data suggest that viral spread may be a consequence of the replication process. Observation point out that expression of proteins from the mp sgp is temporary regulated, and degradation of the proteins occurs rapidly or more slowly, depending on protein stability. It is suggested that the MP contains an external degradation signal that contributes to rapid degradation of the protein even if expressed from the constitutive cp sgp. Experiments conducted to determine whether the degradation of GFP and MP:GFP was regulated at the protein or RNA level, indicated that regulation was at the protein level. RNA accumulation in infected protoplast was not always in correlation with protein accumulation, indicating that other mechanisms together with RNA production determine the final intensity and stability of the fluorescent proteins.
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2

Czajkowski, C. Survey of boric acid corrosion of carbon steel components in nuclear plants. Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/6769556.

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