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1

Dimitrova, D. S., and D. M. Gilbert. "Regulation of mammalian replication origin usage in Xenopus egg extract." Journal of Cell Science 111, no. 19 (October 1, 1998): 2989–98. http://dx.doi.org/10.1242/jcs.111.19.2989.

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Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese hamster ovary (CHO) cell nuclei are introduced into Xenopus egg extracts. At concentrations of intact late-G1-phase nuclei that approximate early Xenopus embryos, the entire genome was duplicated nearly 4 times faster than in culture, accompanied by a de-localization of initiation sites at the dihydrofolate reductase (DHFR) locus. As the concentration of nuclei was increased, the number of initiation sites per nucleus decreased and initiation at the DHFR locus became localized to the physiologically utilized DHFR origin. Origin specification was optimal at nuclear concentrations that approximate the Xenopus mid-blastula transition (MBT). Higher concentrations resulted in an overall inhibition of DNA synthesis. By contrast, with intact early G1-phase nuclei, replication initiated at apparently random sites at all concentrations, despite an identical relationship between nucleo-cytosolic ratio and replicon size. Furthermore, permeabilization of late-G1-phase nuclei, using newly defined conditions that preserve the overall rate of replication, eliminated site-specificity, even at nuclear concentrations optimal for DHFR origin recognition. These data show that both nucleo-cytosolic ratio and nuclear structure play important but independent roles in the regulation of replication origin usage. Nucleo-cytosolic ratio clearly influences the number of replication origins selected. However, titration of cytosolic factors is not sufficient to focus initiation to specific sites. An independent mechanism, effecting changes within G1-phase nuclei, dictates which of many potential initiation sites will function as an origin.
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2

Dimitrova, Daniela S., and David M. Gilbert. "Regulation of mammalian replication origin usage in Xenopus egg extract." Journal of Cell Science 111, no. 19 (January 10, 1998): 2989–98. http://dx.doi.org/10.1242/jcs.19.111.2989.

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ABSTRACT Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese hamster ovary (CHO) cell nuclei are introduced into Xenopus egg extracts. At concentrations of intact late-G1-phase nuclei that approximate early Xenopus embryos, the entire genome was duplicated nearly 4 times faster than in culture, accompanied by a de-localization of initiation sites at the dihydrofolate reductase (DHFR) locus. As the concentration of nuclei was increased, the number of initiation sites per nucleus decreased and initiation at the DHFR locus became localized to the physiologically utilized DHFR origin. Origin specification was optimal at nuclear concentrations that approximate the Xenopus mid-blastula transition (MBT). Higher concentrations resulted in an overall inhibition of DNA synthesis. By contrast, with intact early G1-phase nuclei, replication initiated at apparently random sites at all concentrations, despite an identical relationship between nucleo-cytosolic ratio and replicon size. Furthermore, permeabilization of late-G1-phase nuclei, using newly defined conditions that preserve the overall rate of replication, eliminated site-specificity, even at nuclear concentrations optimal for DHFR origin recognition. These data show that both nucleo-cytosolic ratio and nuclear structure play important but independent roles in the regulation of replication origin usage. Nucleo-cytosolic ratio clearly influences the number of replication origins selected. However, titration of cytosolic factors is not sufficient to focus initiation to specific sites. An independent mechanism, effecting changes within G1-phase nuclei, dictates which of many potential initiation sites will function as an origin.
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3

Liu, Yewei, Sarah J. Russell, and Martin F. Schneider. "Foxo1 nucleo-cytoplasmic distribution and unidirectional nuclear influx are the same in nuclei in a single skeletal muscle fiber but vary between fibers." American Journal of Physiology-Cell Physiology 314, no. 3 (March 1, 2018): C334—C348. http://dx.doi.org/10.1152/ajpcell.00168.2017.

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Foxo transcription factors promote protein breakdown and atrophy of skeletal muscle fibers. Foxo transcriptional effectiveness is largely determined by phosphorylation-dependent nucleo-cytoplasmic shuttling. Imaging Foxo1-green fluorescent protein (GFP) over time in 124 nuclei in 68 multinucleated adult skeletal muscle fibers under control culture conditions reveals large variability between fibers in Foxo1-GFP nucleo-cytoplasmic concentration ratio (N/C) and in the apparent rate coefficient ( kI′) for Foxo1-GFP unidirectional nuclear influx (measured with efflux blocked by leptomycin B). Pairs of values of N/C or of kI′ from different nuclei in the same fiber were essentially the same, but only weakly correlated in nuclei from different fibers in the same culture well. Thus, fiber to fiber variability of cellular factors, but not extracellular factors, determines Foxo1 distribution. Over all nuclei, N/C and kI′ were closely proportional, indicating that kI′ is the major determinant of Foxo1 distribution. IGF-I activation of Foxo kinase Akt reduces variability by decreasing kI′ and N/C in all fibers. However, inhibiting Akt did not drive kI′ uniformly high, indicating other pathways in Foxo1 regulation.
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4

Aiken, Catherine E. M., Peter P. L. Swoboda, Jeremy N. Skepper, and Martin H. Johnson. "The direct measurement of embryogenic volume and nucleo-cytoplasmic ratio during mouse pre-implantation development." Reproduction 128, no. 5 (November 2004): 527–35. http://dx.doi.org/10.1530/rep.1.00281.

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After fertilization, the mammalian conceptus undergoes cleavage, a process of cell proliferation in the absence of interphase growth. It is not known when cleavage ends and gives way to fully replicative cell cycles with a stable nucleo-cytoplasmic ratio. We have used two-photon excitation and confocal microscopy to measure directly volumes and nucleo-cytoplasmic ratios of whole murine concepti and their individual constituent blastomeres during pre-implantation development up to the early uterine attachment stage (day 5). We show that the total cytoplasmic volume of the conceptus remains constant during pre-implantation development, and that the average nucleo-cytoplasmic ratio increases exponentially throughout the same period. Data from individual blastomeres show that both volume and nucleo-cytoplasmic ratio diverge in the inner and outer subpopulations evident from the 16-cell stage (fifth developmental cycle) onwards. Cells from emergent outer trophoblast populations are larger and have smaller nucleo-cytoplasmic ratios than those from emergent inner pluriblast populations. Moreover, the nucleo-cytoplasmic ratio of the trophoblast appears to be stabilizing, suggesting that for this subpopulation cleavage may end at the 16–32-cell transition. Putative hypoblast and epiblast cell subpopulations within the pluriblast were not distinguishable by volume or nucleo-cytoplasmic ratio. Embryonic stem cell volume was higher than that of either cell subpopulation of expanded blastocysts, and their nucleo-cytoplasmic ratio was similar to that of trophoblast cells.
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5

Ocheretina, Rufina Yur'evna, M. V. Stogov, R. Yu Ocheretina, and M. V. Stogov. "Morphometric Indices of Hepatocytes in Shin Bones Injury." N.N. Priorov Journal of Traumatology and Orthopedics 18, no. 3 (September 15, 2011): 76–78. http://dx.doi.org/10.17816/vto201118376-78.

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Morphometric indices of mononuclear hepatocytes (volumes of cells and nuclei, nucleo-cytoplasmic index, percentage of parenchymal cells of different classes) in peripheral and central zones of hepatic lobules were determined 3 days after experimental fracture of shin bones in CBA mice. Increase of hepatocyte nuclei size within the range of K3, K4 and K5 classes was revealed. This is indicative of the involvement of hepatic parenchymal cells into the process of adaptive reorganization of the organism in loco-motor system injury.
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6

Mazzocco, Marco. "Production and Separation of Radioactive Ion Beams." EPJ Web of Conferences 227 (2020): 01014. http://dx.doi.org/10.1051/epjconf/202022701014.

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Radioactive nuclei play an important role in many astrophysical scenarios,from the Big-Bang Nucleo-synthesis to the standard solar model, from quiescent burning to the most explosive events that can occur in our universe. A huge effort has been made for more than thirty years to construct facilities able to deliver beams of radioactive nuclei with increasing intensity and better quality. This contribution revises the different mechanisms and the separation techniques employed for the production of Radioactive Ion Beams.
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7

Bulyk, R., O. Smetaniuk, T. Bulyk, and M. Kryvchanska. "Morphometric analysis of supraoptic neurons of the rat hypothalamic nuclei under conditions of prolonged illumination." Journal of Education, Health and Sport 11, no. 10 (October 29, 2021): 215–20. http://dx.doi.org/10.12775/jehs.2021.11.10.019.

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The article reviews the results of studies of the morphofunctional state of neurons of the supraoptic nuclei of the rat hypothalamus under conditions of different duration of light regime. Under standard light regime in rats, a diurnal rhythm of morphofunctional activity of supraoptic nucleus neurons with maximum activity during daytime (before 2 p.m.) is recorded. In animals subjected to prolonged light exposure, more pronounced changes in the morphofunctional state of the supraoptic neurons of the hypothalamus at 2 a.m. than at 2 p.m. were established. Thus, the neuronal nucleus area was 94.08 ± 9.55 μm2 and was significantly greater than that in intact animals. The nucleo-cytoplasmic ratio of supraoptic hypothalamic neuron at 2 a.m. was lower than that in intact animals due to a decrease in specific nucleus volume. In comparison with the day period (2 p.m.), before 2 a.m. there was revealed a decrease of the neuron body area of supraoptic nuclei of hypothalamus due to possible decrease of the area of nucleus and nucleolus of cells. This was the reason for the increase in the nucleo-cytoplasmic ratio in the neurons under observation at night, which was 2.51 ± 0.023 units. Constant light regime did not cause inversion of the rhythm of morphofunctional activity of the neurons under study, the maximum values, as in intact animals, occurred in the daytime observation period.
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8

Zhang Wei-Hong, Niu Zhong-Ming, Wang Feng, Gong Xiao-Bo, and Sun Bao-Hua. "Uncertainties of nucleo-chronometers from nuclear physics inputs." Acta Physica Sinica 61, no. 11 (2012): 112601. http://dx.doi.org/10.7498/aps.61.112601.

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9

Sh I, Magalov, Huseynova Sadagat, Mustafaeva EE, and Bagirova R. "Nucleo CMP Forte™ for the treatment and rehabilitation of patients with carpal tunnel syndrome." Open Journal of Orthopedics and Rheumatology 8, no. 1 (January 18, 2023): 001–7. http://dx.doi.org/10.17352/ojor.000046.

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Introduction: Carpal Tunnel Syndrome (CTS) is the most common and best well-known cause of peripheral nerve compression. To date, none of the studies have determined the optimal combination of pyrimidine nucleotides and electrical stimulation (ES) in CTS patients. The objective of the study was to evaluate the effectiveness of a novel product containing uridine and cytidine monophosphate (Nucleo CMP Forte™) in combination with ES for the treatment of CTS. Methods: This open-label, randomized, controlled study involved 60 patients with CTS at the Azerbaijan Medical University (Baku, Azerbaijan) and Research Institute of Medical Rehabilitation between 2017 and 2021 years. Patients were randomized to receive the exploratory treatment (Nucleo CMP Forte™ and ES) or single ES treatment for ten days. The combination treatment included two stages: Nucleo CMP Forte™ and ES for ten days (stage one), and Nucleo CMP Forte™ as monotherapy (stage two) for ten days. Results: In the exploratory group, the complete restoration of pain sensitivity was achieved by 17.1% of patients and the narrowing of the existing zone of hypesthesia by 74.3%. Positive Tinel test was revealed in 52.4% of patients after the first and 76.2% after the second stage in the exploratory group, versus 43.8% in the control group. Mean values in the Boston Carpal Tunnel Questionnaire significantly decreased in both groups. Conclusion: Nucleo CMP Forte™ in combination with ES contributes to a more pronounced regression of patients' complaints, clinical manifestations, severity, and neurophysiological indicators in mild-to-moderate CTS.
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10

Mazzocco, Marco. "RIB production and related experiments at EXOTIC." EPJ Web of Conferences 184 (2018): 01012. http://dx.doi.org/10.1051/epjconf/201818401012.

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Radioactive nuclei have a very deep relevance in many astrophysical scenarios, from the Big Bang nucleo-synthesis to supernova explosions. Several Nuclear Physics laboratories around the world have been constructing large-scale facilities for the production of Radioactive Ion Beams (RIBs). The main production techniques, i.e. In-Flight and Isotope Separation On Line, which will be reviewed in this contribution. In particular, we will concentrate on the production of light weakly-bound RIBs at the facility EXOTIC, located at INFN-LNL (Italy) and we will describe the most recent experiments.
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11

Peskine, Lynda Bellity. "Teatro Nucleo: Group Creation." Drama Review: TDR 29, no. 4 (1985): 53. http://dx.doi.org/10.2307/1145691.

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12

Takahashi, K. "Nucleosynthesis and Nucleo-Cosmochronology." EAS Publications Series 11 (2004): 199–216. http://dx.doi.org/10.1051/eas:2004014.

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13

Janota, Cátia S., Francisco J. Calero-Cuenca, Judite Costa, and Edgar R. Gomes. "SnapShot: Nucleo-cytoskeletal Interactions." Cell 169, no. 5 (May 2017): 970–970. http://dx.doi.org/10.1016/j.cell.2017.05.014.

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14

Marshallsay, Christopher. "Invitro nucleo-cytoplasmic transport." Molecular Biology Reports 20, no. 3 (1995): 163–71. http://dx.doi.org/10.1007/bf00990549.

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15

Chisoi, Anca, Mariana Aşchie, and Manuela Enciu. "Morphometric Characterization of Marginal Zone Lymphoma." ARS Medica Tomitana 21, no. 1 (February 1, 2015): 17–21. http://dx.doi.org/10.1515/arsm-2015-0014.

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Abstract The morphometry in histopathology is used to characterize cell populations belonging to different tissues and to identify differences in their parameters with prognostic implications. To achieve morphometric examination were selected 6 of 8 cases identified as marginal zone lymphoma. For each case analysis was done on five fields, for each field measuring the parameters of 20 cells. The studied parameters were for cytoplasm: cytoplasmic area, maximum and minimum cytoplasmic diameter, cytoplasmic perimeter; for nucleus were measured: nuclear area, minimum and maximum nuclear diameter, nuclear perimeter, nuclear contour index, nuclear ellipticity index, nuclear irregularity index. Also the nucleo-cytoplasmic ratio was calculated in all studied cases. Marginal zone lymphoma is characterized in terms of morphometric parameters by small cytoplasmic and nuclear area, and small nucleo-cytoplasmatic ratio of about 1:1. Nuclear contour index is small, accompanied by a large ellipticity index and an small index of nuclear irregularity. Standard deviations for measured morphometric parameters are variable, having high values for cytoplasmic and nuclear area, highlighting the polymorphic nature of the cells. Morphometric aspects, with corresponding microscopic aspects of large and small lymphocyte proliferation with cleaved and uncleaved nuclei, fit this form of lymphoma in terms of clinical outcome in indolent lymphomas category.
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16

Siew, S., and W. deMendonca-Calaca. "Ultrastructural diagnosis of plasmacytoma on a fine-needle aspirate." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 102–3. http://dx.doi.org/10.1017/s0424820100084818.

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A 36 year old man presented with a mass in the chest and multiple “hot” focal lesions were identified on bone scan. Fine needle aspiration was performed of the chest mass. Routine histology showed the presence of some bundles of dense fibrous tissue and a diffuse infiltration of mononuclear cells, which varied in size and nucleo-cytoplasmic ratio. The smaller cells had eccentric hyperchromatic nuclei. Nucleoli were noted in the larger cells. There was well marked cytoplasmic vacuolation of some of the cells. Mitosis was present. A small fragment of tissue was received for electron microscopy. Examination of 1 μm sections showed trabeculae of medium-large polygonal cells with eccentric nuclei and occasional nucleoli. Some irregularly shaped cells had well marked cytoplasmic vacuolation. Mitotic figures were present.
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17

Freyter, Benjamin M., Mutaz A. Abd Al-razaq, Anna Isermann, Anne Dietz, Omid Azimzadeh, Liesbeth Hekking, Maria Gomolka, and Claudia E. Rübe. "Nuclear Fragility in Radiation-Induced Senescence: Blebs and Tubes Visualized by 3D Electron Microscopy." Cells 11, no. 2 (January 13, 2022): 273. http://dx.doi.org/10.3390/cells11020273.

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Irreparable DNA damage following ionizing radiation (IR) triggers prolonged DNA damage response and induces premature senescence. Cellular senescence is a permanent state of cell-cycle arrest characterized by chromatin restructuring, altered nuclear morphology and acquisition of secretory phenotype, which contributes to senescence-related inflammation. However, the mechanistic connections for radiation-induced DNA damage that trigger these senescence-associated hallmarks are poorly understood. In our in vitro model of radiation-induced senescence, mass spectrometry-based proteomics was combined with high-resolution imaging techniques to investigate the interrelations between altered chromatin compaction, nuclear envelope destabilization and nucleo-cytoplasmic chromatin blebbing. Our findings confirm the general pathophysiology of the senescence-response, with disruption of nuclear lamin organization leading to extensive chromatin restructuring and destabilization of the nuclear membrane with release of chromatin fragments into the cytosol, thereby activating cGAS-STING-dependent interferon signaling. By serial block-face scanning electron microscopy (SBF-SEM) whole-cell datasets were acquired to investigate the morphological organization of senescent fibroblasts. High-resolution 3-dimensional (3D) reconstruction of the complex nuclear shape allows us to precisely visualize the segregation of nuclear blebs from the main nucleus and their fusion with lysosomes. By multi-view 3D electron microscopy, we identified nanotubular channels formed in lamin-perturbed nuclei of senescent fibroblasts; the potential role of these nucleo-cytoplasmic nanotubes for expulsion of damaged chromatin has to be examined.
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18

Jin, Lu, Guobin Zhang, Guixiao Yang, and Jiaqiang Dong. "Identification of the Karyopherin Superfamily in Maize and Its Functional Cues in Plant Development." International Journal of Molecular Sciences 23, no. 22 (November 15, 2022): 14103. http://dx.doi.org/10.3390/ijms232214103.

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Appropriate nucleo-cytoplasmic partitioning of proteins is a vital regulatory mechanism in phytohormone signaling and plant development. However, how this is achieved remains incompletely understood. The Karyopherin (KAP) superfamily is critical for separating the biological processes in the nucleus from those in the cytoplasm. The KAP superfamily is divided into Importin α (IMPα) and Importin β (IMPβ) families and includes the core components in mediating nucleocytoplasmic transport. Recent reports suggest the KAPs play crucial regulatory roles in Arabidopsis development and stress response by regulating the nucleo-cytoplasmic transport of members in hormone signaling. However, the KAP members and their associated molecular mechanisms are still poorly understood in maize. Therefore, we first identified seven IMPα and twenty-seven IMPβ genes in the maize genome and described their evolution traits and the recognition rules for substrates with nuclear localization signals (NLSs) or nuclear export signals (NESs) in plants. Next, we searched for the protein interaction partners of the ZmKAPs and selected the ones with Arabidopsis orthologs functioning in auxin biosynthesis, transport, and signaling to predict their potential function. Finally, we found that several ZmKAPs share similar expression patterns with their interacting proteins, implying their function in root development. Overall, this article focuses on the Karyopherin superfamily in maize and starts with this entry point by systematically comprehending the KAP-mediated nucleo-cytoplasmic transport process in plants, and then predicts the function of the ZmKAPs during maize development, with a perspective on a closely associated regulatory mechanism between the nucleo-cytoplasmic transport and the phytohormone network.
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19

Słońska, A., J. Cymerys, J. Skwarska, A. Golke, and M. W. Bańbura. "Influence of importin α/β and exportin 1 on equine herpesvirus type 1 (EHV-1) replication in primary murine neurons." Polish Journal of Veterinary Sciences 16, no. 4 (December 1, 2013): 749–51. http://dx.doi.org/10.2478/pjvs-2013-0106.

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Abstract Viruses replicating in the nucleus need to cross the nuclear membrane barrier during infection, therefore disruption of specific nuclear transport pathways is crucial for their replication cycle. In the present study we have investigated the influence of nucleo-cytoplasmic transport inhibitors - ivermectin and leptomycin B, on EHV-1 replication in primary murine neurons. Obtained results suggest that the examined proteins - exportin 1 and importin α/β may participate, but are not required, during EHV-1 infection. Based on these results, it can be assumed that EHV-1 is able to use other receptors for nucleo-cytoplasmic transport.
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20

Civitarese, Osvaldo, and Mercedes Mosquera. "Nuclear and particle physics aspects of Big Bang nucleo-synthesis." Journal of Physics: Conference Series 322 (October 31, 2011): 012005. http://dx.doi.org/10.1088/1742-6596/322/1/012005.

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21

Zhang, Zhiyuan. "Analysis of the development of an STM32-based smartwatch." Applied and Computational Engineering 31, no. 1 (January 22, 2024): 43–51. http://dx.doi.org/10.54254/2755-2721/31/20230120.

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Sensors and microcontrollers are well-functioning and inexpensive, and the smartwatch industry continues to grow. In response to the problems of traditional smartwatches, which are not fully functional, have poor computing effectiveness and are not suitable for wearing, this thesis designs a smartwatch based on the STMicroelectronics NUCLEO-L476RG microcontroller, using Altium Designer 17 software to draw The schematic diagram and Printed Circuit Board are drawn using Altium Designer 17 software, the driver code of the sensor is compiled and integrated using MBED software, the signal is processed by the STMicroelectronics NUCLEO-L476RG microcontroller and displayed in the Organic Light-Emitting Diode with Bluetooth debugger The measured parameters, such as heart rate, ambient temperature, number of steps, latitude and longitude, are displayed in the Organic Light-Emitting Diode with Bluetooth debugger. The results of the study show that the transmission of the signals in the STMicroelectronics NUCLEO-L476RG microcontroller and the display of the signals in the Organic Light-Emitting Diode and Bluetooth debugger have the advantages of small size, perfect functionality and low energy consumption, making it convenient for the user's daily use.
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22

Roviello, Giovanni N., and Domenica Musumeci. "Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine." RSC Advances 6, no. 68 (2016): 63578–85. http://dx.doi.org/10.1039/c6ra08765e.

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23

Walcott, JJ. "The effect of the Triticum timopheevi nucleo-cytoplasmic system on the performance of an F1 hybrid wheat." Australian Journal of Agricultural Research 36, no. 4 (1985): 553. http://dx.doi.org/10.1071/ar9850553.

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Hybrid performance may be affected by the T. timopheevi nucleo-cytoplasmic system for producing hybrid wheat. Its effects were investigated in three experiments which compared the same hybrid genotype produced either in T. aestivum cytoplasm or in T. timopheevi cytoplasm. Few consistent differences were found between the two hybrids. The only significant difference in grain yield was associated with a significantly reduced seed set in the T. timopheevi hybrid, and suggested a likely effect of location on the degree of fertility restoration achieved. The T. timopheevi nucleo-cytoplasmic system appeared to reduce plant stand and test weight, but to improve flour colour and flour protein content relative to the T. aestivum hybrid.
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Barylo, O. S., S. S. Polishchuk, R. L. Furman, and T. R. Zakalata. "Efficacy of Nucleo C. M. P. Forte in Restorative Therapy after Nerve Injury." Likarska sprava, no. 5-6 (September 30, 2017): 150–57. http://dx.doi.org/10.31640/ls-2017(5-6)27.

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Experimental modelling of nerve injury was carried out by its clamping and subsequent treat- ment with drug product Nucleo C. M. P. Forte. Ultrastructural study of femoral nerve after clamping detected significant dystrophic and destructive changes in axoplasm of axis cylinders of nerve fibers in rats not treated with the drug. Treatment with Nucleo C. M. P. Forte was found to be associated with significantly less pronounced ultrastructural changes as compared to the rat sreceiving no treatment after femoral nerve clamping. Taking into consideration all abovementioned ultrastructural morphological signs, it can be confidently stated that this drug product improves the recovery of nerve fiber, actingas a neuroprotective and restorative agentat tissue and cellular levels.
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Viau, Muriel, Laurène Sonzogni, Mélanie L. Ferlazzo, Elise Berthel, Sandrine Pereira, Larry Bodgi, Adeline Granzotto, et al. "DNA Double-Strand Breaks Induced in Human Cells by Twelve Metallic Species: Quantitative Inter-Comparisons and Influence of the ATM Protein." Biomolecules 11, no. 10 (October 5, 2021): 1462. http://dx.doi.org/10.3390/biom11101462.

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Despite a considerable amount of data, the molecular and cellular bases of the toxicity due to metal exposure remain unknown. Recent mechanistic models from radiobiology have emerged, pointing out that the radiation-induced nucleo-shuttling of the ATM protein (RIANS) initiates the recognition and the repair of DNA double-strand breaks (DSB) and the final response to genotoxic stress. In order to document the role of ATM-dependent DSB repair and signalling after metal exposure, we applied twelve different metal species representing nine elements (Al, Cu, Zn Ni, Pd, Cd, Pb, Cr, and Fe) to human skin, mammary, and brain cells. Our findings suggest that metals may directly or indirectly induce DSB at a rate that depends on the metal properties and concentration, and tissue type. At specific metal concentration ranges, the nucleo-shuttling of ATM can be delayed which impairs DSB recognition and repair and contributes to toxicity and carcinogenicity. Interestingly, as observed after low doses of ionizing radiation, some phenomena equivalent to the biological response observed at high metal concentrations may occur at lower concentrations. A general mechanistic model of the biological response to metal exposure based on the nucleo-shuttling of ATM is proposed to describe the metal-induced stress response and to define quantitative endpoints for toxicity and carcinogenicity.
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Modliński, Jacek A., Jean-Pierre Ozil, Marta K. Modliński, Alina Szarska, Michael A. Reed, Thomas E. Wagner, and Jolanta Karasiewicz. "Development of single mouse blastomeres enlarged to zygote size in conditions of nucleo-cytoplasmic synchrony." Zygote 10, no. 4 (October 31, 2002): 283–90. http://dx.doi.org/10.1017/s096719940200401x.

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The following blastomeres were enlarged to the size of the zygote by one, two or three rounds of blastomere enucleation and electrofusion: (1) from the 2-cell stage (referred to as 2/1 embryos), (2) from the 4-cell stage (referred to as 4/1 embryos), (3) from the 8-cell stage (referred to as 8/1 embryos). Such single enlarged blastomeres developed into blastocysts in vivo in 55.5% (2/1), 28% (4/1) and 6.6% (8/1) of cases. Their mean cell numbers were 45.3, 24.5 and 13.0 in 2/1, 4/1 and 8/1 embryos, respectively. When a blastomere nucleus from another mouse strain (heterologous nucleus) was substituted for a blastomere's own (homologous) one, then fewer blastocysts were formed from 2/1 embryos (34.6%), but not from 4/1 and 8/1 embryos. Five young (10.4%) were born from 2/1 embryos with a homologous nucleus, and nine (8.3%) from 2/1 embryos with heterologous nuclei. Four young (7.1%) were born from 4/1 embryos with heterologous nuclei. No young were obtained from 8/1 embryos. Incorrect cavitation resulting in trophoblastic vesicles and false blastocyst formation was common in 4/1 embryos (18.7% of those with homologous nuclei and 41.3% with heterologous nuclei) and in 8/1 embryos (53.3% and 43.7%, respectively). The results show that neither enlargement to zygote size nor nucleo-cytoplasmic synchrony improve postimplantation development of 4- and 8-cell stage blastomeres when compared with less enlarged non-synchronous ones; therefore, it appears that an insufficient number of inner cell mass cells in blastocysts and not too small a size of isolated blastomeres precludes their postimplantation development.
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27

Vartiainen, Maria K. "Nucleo-cytoplasmic actin relationships in Stockholm." Nucleus 3, no. 2 (March 2012): 123–25. http://dx.doi.org/10.4161/nucl.19515.

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28

Xie, Juan, and Olivier Noel. "Synthesis of Nucleo Aminooxy Acid Derivatives." Synthesis 45, no. 01 (November 23, 2012): 134–40. http://dx.doi.org/10.1055/s-0032-1317689.

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Takahashi, Kohji. "Nucleo-cosmochronology and chemical evolution modellings." Nuclear Physics A 718 (May 2003): 325–32. http://dx.doi.org/10.1016/s0375-9474(03)00734-6.

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30

Society, Argentinian Chesterton. "Nucleo Chestertoniano Argentino pursues Chesterton’s Beatification." Chesterton Review 39, no. 1 (2013): 208–10. http://dx.doi.org/10.5840/chesterton2013391/229.

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31

Gil, Miguel Ángel Espeche. "Nucleo Chestertoniano Argentino pursues Chesterton’s Beatification." Chesterton Review 39, no. 3 (2013): 172–74. http://dx.doi.org/10.5840/chesterton2013393/4116.

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32

Takahata, Naoyuki. "Introgression of extranuclear genomes in finite populations: nucleo-cytoplasmic incompatibility." Genetical Research 45, no. 2 (April 1985): 179–94. http://dx.doi.org/10.1017/s0016672300022102.

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SummaryA ‘two locus two allele’ model is developed with special reference to the introgression of extranuclear genomes between two species of finite size. The model assumes that one locus, coded by a nuclear genome, causes the reproductive barrier while the other locus, coded by an extranuclear genome, causes nucleo-cytoplasmic incompatibility in particular genotypes. To fully study this model, simulations are conducted, and a diffusion equation is derived when introgression or extranuclear gene flow occurs in one direction. It is shown that although selection against the nuclear genome can reduce the levels of extranuclear gene flow and retard the introgression process, the dynamics are very similar to those without such selection. In contrast, the nucleo-cytoplasmic incompatibility directly affects the dynamics of introgressing extranuclear genomes: in large populations the ability of incompatibility to overcome extranuclear gene flow is conspicuous, but in small populations it is overshadowed by random sampling drift. Paternal leakage of extranuclear genomes, if present, is of evolutionary importance only when the male's migration rate is much larger than the female's. When the sizes of two populations are unequal, the introgression is most likely to occur from the larger population to the smaller one in the absence of mating preferences of backcross progeny. Recent observations on interspecific mitochondrial transfer in various species do not support the ubiquitousness of nucleo-cytoplasmic incompatibility as an efficient reproductive barrier.
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33

Guseinova, Sadagat Ganbarovna, S. S. Imamverdieva, E. E. Mustafaeva, M. Yu Mamedova, and K. N. Yusifova. "EFFICIENCY OF APPLICATION OF INTERFERENCY THERAPY IN COMPLEX WITH PYRIMIDINE NUCLEOTIDES IN PATIENTS WITH VERTEBROGENIC RADICULOPATHIES." Russian Journal of Physiotherapy, Balneology and Rehabilitation 16, no. 6 (December 15, 2017): 325–30. http://dx.doi.org/10.18821/1681-3456-2017-16-5-325-330.

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Clinical-neurophysiological bases of the combined use of Nucleo CMF forte preparation and interference therapy in the complex treatment of patients with vertebrogenic radiculopathies were studied. The beneficial effect of this therapeutic complex on the clinical course of the disease, more pronounced analgesic effect and regression of clinical manifestations, as well as improvement of quality of life indicators were proved. It has been established that the therapeutic effect of the complex application of Nucleo CMF forte and interference therapy lies in the improvement in the afferent and efferent links of the neuromotor apparatus, as well as the functional state of the spinal alpha-motoneurons associated with the acceleration of the regenerative processes. Activation of regenerative processes can also be associated with the restoration of certain morphological elements of the nervous system under the action of Nucleo CMF forte, as well as the normalization of the synthesis of complex lipids involved in cellular metabolism and synthesis of substances for the structural restoration of peripheral nerves. Carrying out neurotropic pharmacotherapy in combination with physiotherapy helps optimize therapeutic tactics in patients with vertebrogenic radiculopathies and is aimed at improving the quality of treatment, reducing the using of number of non-steroidal anti-inflammatory drugs and the frequency of relapse of the disease, and improving the quality of life of patients.
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Baek, Kiheon, Alexander David Noblett, Pengyu Ren, and Laura J. Suggs. "Self-assembled nucleo-tripeptide hydrogels provide local and sustained doxorubicin release." Biomaterials Science 8, no. 11 (2020): 3130–37. http://dx.doi.org/10.1039/d0bm00134a.

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Doxorubicin is intercalated within the nanofibril structure of self-assembled nucleo-peptide hydrogels and injection leads to decrease in tumor volume and greater concentration of chemotherapeutic relative to soluble form.
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35

Roviello, Giovanni N., and Domenica Musumeci. "Correction: Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine." RSC Advances 6, no. 82 (2016): 78486. http://dx.doi.org/10.1039/c6ra90072k.

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Correction for ‘Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine’ by Giovanni N. Roviello et al., RSC Adv., 2016, 6, 63578–63585.
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36

Othman, Ahlam Ahmed Mohamed. "Beyond Comparative Poetics: An Evaluation of The Nucleo-genre Paradigm." Journal of Advanced Research in Social Sciences 6, no. 2 (May 15, 2023): 38–47. http://dx.doi.org/10.33422/jarss.v6i2.829.

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The dominance of Western theories in an age of globalization and swift technological advances, which brought to collective consciousness manifestations of literary and artistic genres in distant geographical places that were once viewed as primitive or exotic, necessitates raising the question of comparative poetics anew. During the second half of the twentieth century, scholars such as James J.Y. Liu, Earl Miner, Stephen Owen, and Wai-lim Yip engaged in comparing Western to Eastern poetics. However, their work fell short of reaching a unifying approach to the study of world poetics. This is the gap that Alaa Abd al-Hadi, a thinker, poet and postcolonial critic, came to fill out. Abd al-Hadi’s Nucleo-genre Paradigm is universally oriented. Based on qualitative logic and Lutfi Zadeh's fuzzy sets, it posits that there are essential elements common to various cultural manifestations of a single genre across time and space. It is these common elements, which help decide whether a certain manifestation belongs to a certain genre, that allow for cross cultural communication and understanding while respecting cultural specificities, manifested by an infinite number of aesthetic elements. Unlike the other approaches to the field of comparative poetics, Nucleo-genre Paradigm distinguishes between two levels of genre, the poetic level of production and the aesthetic level of reception, and gives primacy to reception, which is often disregarded in genre theories. In this paper, an attempt is made to evaluate the contribution of the Nucleo-genre Paradigm beyond comparative poetics.
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Santos, Julianna Maria, Raul Martínez-Zaguilán, Arnoldo Rocha Facanha, Fazle Hussain, and Souad R. Sennoune. "Vacuolar H+-ATPase in the nuclear membranes regulates nucleo-cytosolic proton gradients." American Journal of Physiology-Cell Physiology 311, no. 4 (October 1, 2016): C547—C558. http://dx.doi.org/10.1152/ajpcell.00019.2016.

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The regulation of the luminal pH of each organelle is crucial for its function and must be controlled tightly. Nevertheless, it has been assumed that the nuclear pH is regulated by the cytoplasmic proton transporters via the diffusion of H+ across the nuclear pores because of their large diameter. However, it has been demonstrated that ion gradients exist between cytosol and nucleus, suggesting that the permeability of ions across the nuclear pores is restricted. Vacuolar H+-ATPase (V-H+-ATPase) is responsible for the creation and maintenance of trans-membrane electrochemical gradient. We hypothesize that V-H+-ATPase located in the nuclear membranes functions as the primary mechanism to regulate nuclear pH and generate H+ gradients across the nuclear envelope. We studied the subcellular heterogeneity of H+ concentration in the nucleus and cytosol using ratio imaging microscopy and SNARF-1, a pH indicator, in prostate cells. Our results indicate that there are proton gradients across the nuclear membranes that are generated by V-H+-ATPase located in the outer and inner nuclear membranes. We demonstrated that these gradients are mostly dissipated by inhibiting V-H+-ATPase. Immunoblots and V-H+-ATPase activity corroborated the existence of V-H+-ATPase in the nuclear membranes. This study demonstrates that V-H+-ATPase is functionally expressed in nuclear membranes and is responsible for nuclear H+ gradients that may promote not only the coupled transport of substrates, but also most electrochemically driven events across the nuclear membranes. This study represents a paradigm shift that the nucleus can regulate its own pH microenvironment, providing new insights into nuclear ion homeostasis and signaling.
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Chen, Jerry C., Iris Sloan, Alexandra G. Bermudez, Jimmy Hu, and Neil Lin. "Nucleo-cytoskeletal coupling leads to anti-correlation between cytoplasmic and nuclear strains." Biophysical Journal 123, no. 3 (February 2024): 128a. http://dx.doi.org/10.1016/j.bpj.2023.11.884.

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39

Martinez-Zaguilan, Raul, Juliana Santos, Arnoldo Facanha, and Souad R. Sennoune. "Vacuolar H+-ATPase in the Nuclear Membranes Regulate Nucleo-Cytosolic Proton Gradients." Biophysical Journal 116, no. 3 (February 2019): 170a. http://dx.doi.org/10.1016/j.bpj.2018.11.945.

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40

Minola, Valeria, and Anna Paschetta. "L'origine multifattoriale nei disturbi dell'apprendimento: un protocollo terapeutico con la famiglia." TERAPIA FAMILIARE, no. 126 (November 2021): 45–65. http://dx.doi.org/10.3280/tf2021-126004.

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In questo articolo presentiamo come la terapia sistemico familiare-individuale sia effi cace nella presa in carico di un nucleo familiare che vive dinamiche relazionali complesse, altrimenti messe in ombra da una diagnosi di disturbo dell'apprendimento a carico del fi glio e dalle ricadute del disagio prettamente in ambito scolastico. Presso il nostro studio, per i ragazzi e per le loro famiglie che ci consultano, lavoriamo adattando il modello proposto dalla scuola di Psicoterapia Mara Selvini Palazzoli. Dopo un'iniziale spiegazione sulle caratteristiche del disturbo specifi co dell'apprendimento, presenteremo la teoria della multifattorialità all'origine del DSA di Simonetta, che assumiamo come cornice teorica. Questo modello si basa sugli studi delle neuroscienze, delle teorie dell'attaccamento e della psicotraumatologia che permettono la lettura dell'eziopatogenesi del sintomo analizzando diversi livelli di complessità. Nel nostro metodo di intervento, rileviamo con particolare attenzione il signifi cato che il sintomo assume quando si esprime in un particolare contesto relazionale. Per questo dove compare un disturbo dell'apprendimento riteniamo utile indagare gli attaccamenti e lo sviluppo delle personalità dei componenti della famiglia. Inoltre aiutiamo il nucleo a rileggere le proprie dinamiche relazionali in un'ottica sistemica e approfondiamo le dinamiche passate secon do la prospettiva trigenerazionale che le sottende, stando attente a rilevare l'eventuale presenza di traumi non elaborati nelle storie di sviluppo dei componenti della famiglia. Inoltre evidenziamo l'importanza dello stile di conduzione che prevede l'utilizzo delle tecniche verbali, non verbali e i diversi formati di convocazione. Presentiamo il nostro metodo di lavoro attraverso la descrizione della presa in carico psicoterapeutica di un nucleo familiare.
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Sazonova, E. N., O. A. Lebed’ko, G. A. Denisyuk, K. V. Zhmerenetskiy, and V. A. Dobrykh. "Cytoprotective effect of non-opioid leu-enkephalin analogue in primary culture of pulmonary fibroblasts in oxidative stress." Kazan medical journal 100, no. 1 (December 15, 2019): 153–57. http://dx.doi.org/10.17816/kmj2019-153.

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Aim. Analysis of the cytoprotective effect of non-opioid leu-enkephalin analogue (Phe-D-Ala-Gly-Phe-Leu-Arg) in the primary culture of pulmonary fibroblasts in conditions of oxidative stress. Methods. Pulmonary fibroblasts were incubated with the peptide of non-opioid leu-enkephalin analogue (Phe-D-Ala-Gly-Phe-Leu-Arg) in the concentration 0.1 μM for 6 hours. To simulate oxidative stress, 60 μM H2O2 was added to the culture medium for 2 hours. Experimental series included (1) «control»; (2) «non-opiate leu-enkephalin analogue» (the peptide was added to the culture medium 44 hours after the final passage); (3) «oxidative stress» (H2O2 was added to the culture medium 48 hours after the final passage); (4) «non-opiate leu-enkephalin analogue + oxidative stress» (the peptide and H2O2 were added to the culture medium 44 and 48 hours respectively after the final passage). In order to evaluate the generation of superoxide anion by pulmonary fibroblasts, the method of lucigenin-dependent chemiluminescence was used. Computer morphometry of the nucleo-nucleolar apparatus of fibroblasts stained with silver nitrate by the AgNOR method was used to assess the cell state: the area of fibroblast nuclei, the total nucleoli area in the nucleus, and the number of nucleoli in the nucleus were measured. These parameters correlate with the activity of anabolic processes in the cells. Results. The effect of H2O2 on the primary culture of pulmonary fibroblasts caused an increase of superoxide anion generation by the fibroblasts, reduction of fibroblast nuclei size, decrease of nucleoli amount and size. Pre-incubation of pulmonary fibroblasts with a non-opioid leu-enkephalin analogue reduced the H2O2-induced generation of superoxide anion, corrected changes in the nucleo-nucleolar apparatus of fibroblasts caused by oxidative stress. In our previous studies, similar effect in the same model was shown for non-selective μ/δ-opioid receptor agonist peptide sedatin (dermorphin analogue). The mechanism of cytoprotective effect of non-opioid leu-enkephalin analogue may include the affinity of this peptide to nociceptin receptors (NOR receptors) that requires further studies. Conclusion. The results of the study indicate a direct cytoprotective effect of the peptide Phe-D-Ala-Gly-Phe-Leu-Arg (non-opioid leu-enkephalin analogue) in oxidative stress.
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42

Yang, Yan, and YaWei Shi. "Spectrin-like domain 2 of DRP2 serves as a novel binding region for the NLS2 and 3 sub-domains of L-periaxin." RSC Advances 5, no. 103 (2015): 84356–66. http://dx.doi.org/10.1039/c5ra12703c.

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The NLS1 domain of L-periaxin involved in nucleo-cytoplasmic shuttling, NLS2 and 3 participated in interaction with spectrin-like domain 2 of DRP2. The binding model of DRP2 and L-periaxin is crucial for understanding the role of L-periaxin in PNS.
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43

Grivell, L. A. "Nucleo-Mitochondrial Interactions in Mitochondrial Gene Expression." Critical Reviews in Biochemistry and Molecular Biology 30, no. 2 (January 1995): 121–64. http://dx.doi.org/10.3109/10409239509085141.

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44

Oliveira, Maria Cleide Ribeiro de, and Gabriela Almeida Araújo. "NEPP - NUCLEO DE EXTENSÃO E PRÁTICA PROFISSIONAL." Revista Diálogos da Extensão 1, no. 1 (December 29, 2015): 48. http://dx.doi.org/10.15628/dialogos.2015.3926.

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O Núcleo de Extensão e Prática Profissional – NEPP – é um projeto, sem fins lucrativos, que surgiu no ano de 2009, com o intuito de suprir as demandas de prática profissional, na área da construção civil para os alunos do IFRN. De acordo com o Projeto Político Pedagógico do IFRN, o NEPP, além de proporcionar a prática profissional aos alunos da DIACON (Diretoria Acadêmica de Construção Civil), também oferece serviços técnicos na área de arquitetura e engenharia civil, de forma gratuita, à comunidade carente, instituições filantrópicas, além de servidores e alunos do Instituto. Os alunos atuantes nesse projeto desenvolvem atividades relativas à sua formação técnica utilizando softwares como o AutoCAD, o Sketchup e o Revit Architecture.
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45

GRIVELL, Leslie A. "Nucleo-mitochondrial interactions in yeast mitochondrial biogenesis." European Journal of Biochemistry 182, no. 3 (July 1989): 477–93. http://dx.doi.org/10.1111/j.1432-1033.1989.tb14854.x.

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46

Kevei, E., E. Schafer, and F. Nagy. "Light-regulated nucleo-cytoplasmic partitioning of phytochromes." Journal of Experimental Botany 58, no. 12 (July 13, 2007): 3113–24. http://dx.doi.org/10.1093/jxb/erm145.

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47

Manta, P., D. Vassilopoulos, and M. Spengos. "Nucleo-cytoplasmic ratio in ageing skeletal muscle." European Archives of Psychiatry and Neurological Sciences 236, no. 4 (April 1987): 235–36. http://dx.doi.org/10.1007/bf00383854.

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48

Fukuhara, Noemi, Elena Fernandez, Judith Ebert, Elena Conti, and Dmitri Svergun. "Conformational Variability of Nucleo-cytoplasmic Transport Factors." Journal of Biological Chemistry 279, no. 3 (October 15, 2003): 2176–81. http://dx.doi.org/10.1074/jbc.m309112200.

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49

Teixeira Jr., Francisco, Per Rigler, and Corinne Vebert-Nardin. "Nucleo-copolymers: oligonucleotide-based amphiphilic diblock copolymers." Chemical Communications, no. 11 (2007): 1130. http://dx.doi.org/10.1039/b617109e.

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50

Greber, Urs F., and Ernesto Carafoli. "Signalling takes control of nucleo‐cytoplasmic trafficking." EMBO reports 3, no. 5 (May 2002): 410–14. http://dx.doi.org/10.1093/embo-reports/kvf093.

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