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Journal articles on the topic 'O-dianisidine'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Jasnowska, J., M. Ligaj, B. Stupnicka, and M. Filipiak. "DNA sensor for o-dianisidine." Bioelectrochemistry 64, no. 1 (2004): 85–90. http://dx.doi.org/10.1016/j.bioelechem.2004.03.004.

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2

L., P. PANDEY, SINGH B., and K. PADHI K. "Spectrophotometric Determination of Vanadium using o-Dianisidine as Reasent." Journal of Indian Chemical Society Vol. 65, Nov 1988 (1988): 817–18. https://doi.org/10.5281/zenodo.6117510.

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Analytical Chemistry Division, National Metallurgical Laboratory, Jamshedpur-331 007 <em>Manuscript received 9 June 1987, revised 20 July 1988, accepted 17 August 1988</em> Spectrophotometric Determination of Vanadium using <em>o</em>-Dianisidine as Reasent.

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3

Sornambikai, Sundaram, Lim Qing Hin, Kasi Marimuthu, and Mohammed Rafiq Abdul Kadir. "Fish embryo toxicity assessment of o-dianisidine in Clarias gariepinus and its electrochemical treatment in aquatic samples using super conductive carbon black." RSC Advances 6, no. 55 (2016): 50255–66. http://dx.doi.org/10.1039/c6ra00158k.

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The present study tested fish embryo toxicity (FET) of o-dianisidine (o-dian) on African catfish (Clarias gariepinus) and its electrochemical treatment through electro-oxidation of real aquatic samples for the first time.

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4

Sundaram, Sornambikai, Madhanagopal Jagannathan, Mohammed Rafiq Abdul Kadir, Sathishkumar Palanivel, Tony Hadibarata, and Abdul Rahim Mohammed Yusoff. "A new electro-generated o-dianisidine derivative stabilized MWCNT-modified GCE for low potential gallic acid detection." RSC Advances 5, no. 57 (2015): 45996–6006. http://dx.doi.org/10.1039/c5ra06304c.

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5

Shiundu, Paul M., Adrian P. Wade, and S. B. Jonnalagadda. "Spectrophotometric determination of peroxydisulphate with o-dianisidine by flow injection." Canadian Journal of Chemistry 68, no. 10 (1990): 1750–56. http://dx.doi.org/10.1139/v90-272.

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Precise and accurate knowledge of peroxydisulfate ion concentration is critical in industrial polymer production, where it is extensively used as an activator. Its ability to oxidize in either acidic, neutral, or alkali media also makes it widely applicable in many other areas of chemistry.In this paper we present an optimized spectrophotometric flow injection method for determination of peroxydisulfate. The analyte oxidizes o-dianisidine to form a stable product which has a convenient absorbance maximum at 450 nm. This provides a simple and sensitive alternative to present methods which are m

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6

Kim, Y. S., and G. F. Combs. "Measurement of ceruloplasmin in chick plasma by o-dianisidine oxidation." Nutrition Research 8, no. 4 (1988): 379–87. http://dx.doi.org/10.1016/s0271-5317(88)80032-0.

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7

Abou-Shoer, Mohamed. "Evaluation of Carbonyl Compounds in Natural Products by o-Dianisidine." Chromatographia 68, no. 5-6 (2008): 447–51. http://dx.doi.org/10.1365/s10337-008-0708-1.

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8

Lopez-Nieves, Mayda, Peter D. Wentzell, and Stanley R. Crouch. "Kinetics of the reaction between aromatic aldehydes and o-dianisidine." Analytical Chemistry 62, no. 3 (1990): 304–8. http://dx.doi.org/10.1021/ac00202a015.

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9

Im, Hansu, Jineun Kim, Changeun Sim, and Tae Ho Kim. "Crystal structure ofN,N′-dibenzyl-3,3′-dimethoxybenzidine." Acta Crystallographica Section E Crystallographic Communications 74, no. 3 (2018): 271–74. http://dx.doi.org/10.1107/s2056989018001688.

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The title compound, (systematic name:N,N′-dibenzyl-3,3′-dimethoxy-1,1′-biphenyl-4,4′-diamine), C28H28N2O2, was synthesized by the reduction of a Schiff base preparedviaa condensation reaction betweeno-dianisidine and benzaldehyde under acidic conditions. The molecule lies on a crystallographic inversion centre so that the asymmetric unit contains one half-molecule. The biphenyl moiety compound is essentially planar. Two intramolecular N—H...O hydrogen bonds occur. The dihedral angle between the terminal phenyl and phenylene rings of a benzidine unit is 48.68 (6)°. The methylene C atom of the b

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10

Evecen, Meryem, Hasan Tanak, Necmi Dege, Mehmet Kara, Onur Erman Dogan, and Erbil Ağar. "Molecular structure, spectroscopic, and density functional theory studies of o-Dianisidine." Molecular Crystals and Liquid Crystals 648, no. 1 (2017): 183–201. http://dx.doi.org/10.1080/15421406.2016.1275300.

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11

Beklemishev, M. "Accelerating effect of silica on the indicator reaction o-dianisidine–H2O2." Talanta 51, no. 2 (2000): 389–94. http://dx.doi.org/10.1016/s0039-9140(99)00302-1.

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12

Hatanaka, Y., Y. Toyoda, and Y. Kitagawa. "Clastogenicity of o-dianisidine in in vitro and in vivo systems." Mutation Research/Environmental Mutagenesis and Related Subjects 292, no. 3 (1993): 265–66. http://dx.doi.org/10.1016/0165-1161(93)90043-y.

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13

Ribeiro, J. M., and R. H. Nussenzveig. "The salivary catechol oxidase/peroxidase activities of the mosquito Anopheles albimanus." Journal of Experimental Biology 179, no. 1 (1993): 273–87. http://dx.doi.org/10.1242/jeb.179.1.273.

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Salivary gland homogenates from adult female Anopheles albimanus mosquitoes relaxed aortic rings preconstricted with noradrenaline (NA). This relaxation is slow and is due to destruction of NA. Incubation of NA with the homogenate yielded a product with a spectrum consistent with the corresponding adrenochrome. Oxidation of NA was enhanced by a superoxide generation system and inhibited by the combined action of superoxide dismutase and catalase. Additionally, peroxidase activity on both synthetic (o-dianisidine) and biologically active (serotonin) substrates was also present in the salivary g

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14

Huidobro, José F., M. Pilar Sánchez, Soledad Muniategui, and M. Teresa Sancho. "Precise Method for the Measurement of Catalase Activity in Honey." Journal of AOAC INTERNATIONAL 88, no. 3 (2005): 800–804. http://dx.doi.org/10.1093/jaoac/88.3.800.

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Abstract An improved method is reported for the determination of catalase activity in honey. We tested different dialysis membranes, dialysis fluid compositions and amounts, dialysis temperatures, sample amounts, and dialysis times. The best results were obtained by dialysis of 7.50 g sample in a cellulose dialysis sack, using two 3 L portions of 0.015M sodium phosphate buffer (pH 7.0) as the dialysis fluid at 4°C for 22 h. As in previous methods, catalase activity was determined on the basis of the rate of disappearance of the substrate, H2O2, with the H2O2 determined spectrophotometrically a

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15

Machuca, Angela, Hiroshi Aoyama, and Nelson Durán. "Production and characterization of thermostable phenol oxidases of the ascomycete Thermoascus aurantiacus." Biotechnology and Applied Biochemistry 27, no. 3 (1998): 217–23. http://dx.doi.org/10.1111/j.1470-8744.1998.tb00497.x.

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The effect of some culture conditions on extracellular phenol oxidase (PO) production by the thermophilic ascomycete Thermoascus aurantiacus was studied. The effect of different lignocellulosic and an aromatic compound (p‐anisidine) as inducers of PO activity was studied in liquid culture. The most effective substrate, wheat bran, induced between 1 and 2 units/ml PO activity when utilized at a concentration of 1.5% (w/v). The type and size of fungal inoculum influenced the PO production. However, the agitation of cultures did not increase PO production. An initial pH between 6 and 8 resulted i

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16

Neumeister, Charles E. "Analysis of Urine to Monitor Exposures to Benzidine, o-Dianisidine, o-Tolidine, and 4, 4′-Methylenedianiline." Applied Occupational and Environmental Hygiene 6, no. 11 (1991): 953–58. http://dx.doi.org/10.1080/1047322x.1991.10387997.

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17

Icardo, M. CatalÃ¡, J. V. GarcÃa Mateo, and J. MartÃnez Calatayud. "o-Dianisidine: a new reagent for selective spectrophotometric, flow injection determination of chlorine." Analyst 126, no. 11 (2001): 2087–92. http://dx.doi.org/10.1039/b107000m.

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18

Sánchez-Pedreño, C., M. Hernández Córdoba, and P. Viñas López-Pelegrín. "Analytical applications of the o-dianisidine-persulfate reaction: Kinetic determination of silver and cobalt." Microchemical Journal 32, no. 2 (1985): 242–48. http://dx.doi.org/10.1016/0026-265x(85)90084-0.

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19

Osuji, Godson O., Wenceslaus C. Madu, Eustace Duffus, et al. "Regulated Lignin Structural Units and Soil Organic Carbon Content by Cowpea Peroxidase." Journal of Agricultural Science 8, no. 9 (2016): 12. http://dx.doi.org/10.5539/jas.v8n9p12.

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<p class="Standard">Peroxidases participate in lignin biosynthesis, but there is no biochemical resolution between the structural units of lignin and soil organic carbon (SOC) contents. Black-eyed beans are staple high-protein foods for millions of at-risk populations in every continent. Its cultivation in semiarid zones could be leveraged to maximize SOC sequestration. Cowpea was treated with stoichiometric mixes of mineral nutrients. Peroxidase was electrophoretically purified from leaves, and assayed for o-dianisidine (guaiacyl units) and pyrogallol (p-hydroxyphenyl units) substrate s

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20

ENTRALA, E., C. MASCARO, and J. BARRETT. "Anti-oxidant enzymes in Cryptosporidium parvum oocysts." Parasitology 114, no. 1 (1997): 13–17. http://dx.doi.org/10.1017/s0031182096008037.

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Oocysts of Cryptosporidium parvum showed relatively low levels of SOD activity. The SOD which had a pI of 4.8 and an approximate molecular weight of 35 kDa appeared to be iron dependent. Catalase, glutathione transferase, glutathione reductase and glutathione peroxidase activity could not be detected, nor could trypanothione reductase. No NADH or NADPH oxidase activity could be detected, nor could peroxidase activity be demonstrated using o-dianisidine, guaiacol, NADPH or NADH as co-substrates. However, an NADPH-dependent H2O2 scavenging system was detected in the insoluble fraction.

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21

Banfi, G., A. Malavazos, E. L. Iorio, et al. "The iron-o-dianisidine/xylenol orange assay in comparative oxidative stress assessment. Some possible shortcomings." European Journal of Applied Physiology 97, no. 4 (2006): 506–8. http://dx.doi.org/10.1007/s00421-006-0204-y.

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22

Molinari, Sergio. "Antioxidant enzymes in (a)virulent populations of root-knot nematodes." Nematology 11, no. 5 (2009): 689–97. http://dx.doi.org/10.1163/156854108x399317.

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AbstractAssays of antioxidant enzymes, including catalase, peroxidase and superoxide dismutase (SOD), have been carried out on extracts of females and second-stage juveniles (J2) of a pair of Meloidogyne incognita isolates, one virulent and one avirulent, selected on tomato, and an avirulent field population of M. incognita. Catalase and SOD activity were found to be higher in extracts of the virulent isolate SM1 when compared with the avirulent counterparts. Peroxidase activity, assayed with o-dianisidine as the substrate, was enhanced in SM1 J2 compared with the avirulent avr1 J2. Catalase i

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23

MUTSUDA, Michinori, Takahiro ISHIKAWA, Toru TAKEDA, and Shigeru SHIGEOKA. "The catalase-peroxidase of Synechococcus PCC 7942: purification, nucleotide sequence analysis and expression in Escherichia coli." Biochemical Journal 316, no. 1 (1996): 251–57. http://dx.doi.org/10.1042/bj3160251.

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Synechococcus PCC 7942, a cyanobacterium, possesses catalase–peroxidase as the sole hydrogen peroxide-scavenging system. The enzyme has been purified to electrophoretic homogenenity from the cells. The native enzyme had a molecular mass of 150 kDa and was composed of two identical subunits of molecular mass 79 kDa. The apparent Km value of the catalase activity for H2O2 was 4.2±0.27 mM and the kcat value was 2.6×104 s-1. The enzyme contained high catalase activity and an appreciable peroxidase activity with o-dianisidine and pyrogallol. The catalase activity was not inhibited by 3-amino-1,2,4-

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24

RAYAMAN, PERVİN, ERKAN RAYAMAN, ADİLE ÇEVİKBAŞ, et al. "Effect of antibiotics on polymorphonuclear leukocyte functions and myeloperoxidase activity, glutathione and malondialdehyde levels in allergic asthma." Polish Journal of Microbiology 64, no. 1 (2015): 69–72. http://dx.doi.org/10.33073/pjm-2015-010.

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We investigated the effect of ciprofloxacin, rifampicine and doxycycline on myeloperoxidase (MPO) activity, glutathione (GSH) and malondialdehyde (MDA) levels in allergic asthma patients and healthy volunteers. Polymorphonuclear leukocytes (PMNs) were isolated with ficoll-hypaque gradient centrifugation method. MPO activity was assayed with modified o-dianisidine, GSH by Ellman's and MDA levels by Beuge's method. PMN functions and MDA levels of patients significantly decreased when compared with healthy volunteers. Ciprofloxacin significantly increased PMN functions, MPO activity and MDA level

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25

S., LASKAR, and SENGUPTA B. "New Spray Reagents for the Detection of Hazardous Phenols separated by Tlc." Journal of Indian Chemical Society Vol. 66, Dec 1989 (1989): 899–901. https://doi.org/10.5281/zenodo.6024222.

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Department of Chemistry, University of Burdwan, Burdwan-713 104 <em>Manuscript received 12 May 1988, accepted 20 July 1989</em> The separation pattern of 22 phenols have been studied on silica gel-G tic plates using chloroform — methanol (98 : 2) as developer. The spots were detected using 4 new spray reagents, viz. orthanilic acid, <em>o</em>-dianisidine and their diazotised products. Most of the phenols gave easily detectable characteristic colours and the detection limit was generally 1-5 μ<em>g, </em>and even as low as 0.1-0.5 μg in most case for the diazotised orthanilic acid.

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26

Okoroafor, Prince Odilichukwu, Eshu Okpashi Victor, Peter-Roins Bayim Bayim, Sabinus Oscar Ezeh, Chima Nwanguma Bennett, and Obioma Uzoma Njoku. "Optimised isolation, partial-purification and profiling of peroxidase extracted from saccharomyces cerevisiae of fresh palm wine." African Journal of Biological Sciences 3, no. 4 (2021): 88–96. https://doi.org/10.33472/AFJBS.3.4.2021.88-96.

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Saccharomyces cerevisiae was obtained from palm wine and used for the extraction of peroxidase. Peroxidase isolated from <em>Saccharomyces cerevisiae</em> was partially purified and characterized. A twelve-day pilot study was carried out which gave the highest activity on day eight. The crude enzyme-specific activity –0.751 U/mg with 70% ammonium sulfate precipitation of peroxidase was achieved. Peroxidase was partially purified with ammonium sulfate precipitation, following gel filtration on Sephadex G-100. Two peaks connoted as fractions (A & B) on the elution profile of the gel fi

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27

Alshammary, Malak, Essam Kotb, Ibtisam M. Ababutain, et al. "Production, Biochemical Characterization, and Application of Laccase from Halophilic Curvularia lunata MLK46 Recovered from Mangrove Rhizosphere." Biology 14, no. 4 (2025): 402. https://doi.org/10.3390/biology14040402.

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Laccase production was evaluated in 108 fungal isolates recovered from the eastern coast of Saudi Arabia, a critical element in environmental biodegradation and biotransformation. The most active isolate was identified as Curvularia lunata MLK46 (GenBank accession no. PQ100161). It exhibited maximal productivity at pH 6.5, 30 °C, and incubation for 5 d, with 1% sodium nitrate and 1% galactose as the preferred nitrogen and carbon sources, respectively. Productivity was enhanced by NaCl, CuSO4, and FeCl3 supplementation, with a maximum at 0.3 mM, 0.2 mM, and 61.7 mM concentrations, respectively.

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28

Kireyko, A. V., I. A. Veselova, and T. N. Shekhovtsova. "Mechanisms of peroxidase oxidation of o-dianisidine, 3,3′,5,5′-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate." Russian Journal of Bioorganic Chemistry 32, no. 1 (2006): 71–77. http://dx.doi.org/10.1134/s1068162006010079.

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29

Stepien, Karolina M., and Mark Guy. "Caeruloplasmin oxidase activity: measurement in serum by use of o-dianisidine dihydrochloride on a microplate reader." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 55, no. 1 (2017): 149–57. http://dx.doi.org/10.1177/0004563217695350.

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Background The enzymatic method of caeruloplasmin measurement is based on copper-dependent oxidase activity. The advantage of the oxidase determination is that it has a much lower detection limit compared with immunoassay-based methods. It has found its application in both the diagnosis of Wilson’s disease and also in the monitoring of patients’ response to treatment. Methods The method previously described in literature was adapted for use on a 96-well plate. Caeruloplasmin oxidase activity results were derived from the equation: caeruloplasmin oxidase activity = (A15−A5) × 185 U/L. Results R

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30

Jin, Xu Dong, Yue Hong Jin, Zhi Gang Cui, Yuan Yuan Li, and Hai Bo Wang. "Synthesis and Characterization of Binuclear Indium(Ⅲ) Complexes with Bis-Schiff Bases Derived from Benzidine or its Derivatives." Advanced Materials Research 197-198 (February 2011): 627–30. http://dx.doi.org/10.4028/www.scientific.net/amr.197-198.627.

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By reactions of indium chloride tetrahydrate with three bis-Schiff bases ligands (N, N’-bis (3-methoxy-salicylidene)benzidine = L1; N, N’-bis (3-methoxysalicylidene)-2, 2’-dimethyl- benzidine =L2; N, N’-bis (3-methoxysalicylidene)-o- dianisidine = L3), respectively, three novel corresponding complexes 1~3 were obtained. The complexes were characterized by infrared spectrum, molar conductance, elemental analysis and thermal gravimetric analysis. The chemical formulae of the complexes 1~3 were determined as (InCl3)2Ln (1, n = 1; 2, n = 2; 3, n = 3). All the complexes are constructed with binucle

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31

Maki, Yuko, and Yutaka Amao. "Novel Optical Solid-State Glucose Sensor Tip Using Immobilized o-Dianisidine, Glucose Oxidase and Peroxidase." Sensor Letters 6, no. 1 (2008): 242–45. http://dx.doi.org/10.1166/sl.2008.016.

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32

Catalá Icardo, M., J. V. Garcı́a Mateo, and J. Martı́nez Calatayud. "Selective chlorine determination by gas diffusion in a tandem flow assembly and spectrophotometric detection with o-dianisidine." Analytica Chimica Acta 443, no. 1 (2001): 153–63. http://dx.doi.org/10.1016/s0003-2670(01)01183-7.

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33

Wise, Ronald W., Terry V. Zenser, and Bernard B. Davis. "Prostaglandin H synthase oxidation of benzidine and o-dianisidine: reduction and conjugation of activated amines by thiols." Carcinogenesis 6, no. 4 (1985): 579–83. http://dx.doi.org/10.1093/carcin/6.4.579.

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34

KO, Omeje, Eze SOO, and FC Chilaka. "Peroxidase from infected fruit of Solanum sp. grown in Nsukka." Bangladesh Journal of Scientific and Industrial Research 54, no. 2 (2019): 131–38. http://dx.doi.org/10.3329/bjsir.v54i2.41669.

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In this study, we characterized the activity of peroxidase a quality control enzyme from the infected fruit of Solanum sp. Peroxidase was purified to homogeneity by ammonium sulfate precipitation, dialysis, ion exchange chromatography and size exclusion chromatography. The molecular weight of the native enzyme was 63000 da. The enzyme was shown to have two iso-enzymes with distinct optimum pH of 4.5 and 7.0 and optimum temperature of 40 and 70⁰C. The purified enzyme had broad substrate specificity with o-dianisidine being the ideal substrate. Na+, Ca2+, Mg2+, Mn2+, Cu2+, Al3+ were shown to be

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35

Oprea, Stefan, Violeta Otilia Potolinca, and Veronica Oprea. "Physical properties and dielectric behavior of the poly(urethane‐urea) based on o ‐dianisidine and renewable cross‐linkers." Journal of Applied Polymer Science 138, no. 21 (2021): 50481. http://dx.doi.org/10.1002/app.50481.

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36

Grases, F., J. G. March, and R. Forteza. "Study of the oxidation of diphenylamine and o-dianisidine by technetium(VII) catalyzed by copper(II). Analytical applications." Journal of Radioanalytical and Nuclear Chemistry Articles 102, no. 1 (1986): 121–29. http://dx.doi.org/10.1007/bf02037953.

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37

Rogozhina, T. V., and V. V. Rogozhin. "Phenothiazines are slowly oxidizable substrates of horseradish peroxidase." Biomeditsinskaya Khimiya 57, no. 5 (2011): 544–53. http://dx.doi.org/10.18097/pbmc20115705544.

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Reactions of peroxidase oxidation of triftazine and thioproperazine have been investigated in the presence of horseradish peroxidase using steady state kinetic methods. It has been shown that phenothiazines are slowly oxidizable substrates for horseradish peroxidase. kcat and Km values have been determined in the range of pH from 4.5 to 7.5. The study of co-oxidation of phenothiazines and o-dianisidine (ODN) revealed that in the presence of aminazine and ODN in the reaction medium both substances follow sequential oxidation. ODN oxidation was not observed until full conversion of aminazine. At

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38

Brun, Antonio, Melisa E. Magallanes, Carlos Martínez del Rio, Gregory A. Barrett-Wilt, William H. Karasov, and Enrique Caviedes-Vidal. "A Fast and Accurate Method to Identify and Quantify Enzymes in Brush-Border Membranes: In Situ Hydrolysis Followed by Nano LC-MS/MS." Methods and Protocols 3, no. 1 (2020): 15. http://dx.doi.org/10.3390/mps3010015.

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A simple method for the identification of brush-border membrane α-glucosidases is described. The proteins were first solubilized and separated in a gel under native, non-denaturing, conditions. The gel was then incubated in substrate solutions (maltose or sucrose), and the product (glucose) exposed in situ by the oxidation of o-dianisidine, which yields a brown-orange color. Nano-liquid chromatography coupled to mass spectrometry analyses of proteins (nano LC-MS/MS) present in the colored bands excised from the gels, was used to confirm the presence of the enzymes. The stain is inexpensive and

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39

SIL, SUSMITA, MANOJ KAR, and ABHAY SANKAR CHAKRABORTI. "Haematoporphyrin enhances the peroxidase activity of haemoglobin." Journal of Porphyrins and Phthalocyanines 04, no. 02 (2000): 168–74. http://dx.doi.org/10.1002/(sici)1099-1409(200003)4:2<168::aid-jpp165>3.0.co;2-o.

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The effect of haematoporphyrin, a component of some of the widely used anticancer drugs, on the peroxidase activity of haemoglobin has been studied. Haematoporphyrin increases the haemoglobin-catalysed hydrogen peroxide-mediated oxidation of o-dianisidine or NADH. Spectrophotometric study reveals that an interaction occurs between haemoglobin and haematoporphyrin which leads to a conformational change of the protein. The extent of enhanced peroxidase activity as well as conformational change of the protein vary in a positive manner with the stoichiometric ratio of haematoporphyrin/haemoglobin.

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40

Savitsky, Alexander P., Mary I. Nelen, Anatoly K. Yatsmirsky, Mary V. Demcheva, Gely V. Ponomarev, and Igor V. Sinikov. "Kinetics of oxidation of o-dianisidine by hydrogen peroxide in the presence of antibody complexes of iron(III) coproporphyrin." Applied Biochemistry and Biotechnology 47, no. 2-3 (1994): 317–27. http://dx.doi.org/10.1007/bf02787943.

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41

Siotto, Mariacristina, Patrizio Pasqualetti, Massimo Marano, and Rosanna Squitti. "Automation of o-dianisidine assay for ceruloplasmin activity analyses: usefulness of investigation in Wilson’s disease and in hepatic encephalopathy." Journal of Neural Transmission 121, no. 10 (2014): 1281–86. http://dx.doi.org/10.1007/s00702-014-1196-0.

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42

Cecchini Gualandi, Stefano, Tommaso Di Palma, and Raffaele Boni. "Analytical Validation of Two Assays for Equine Ceruloplasmin Ferroxidase Activity Assessment." Veterinary Sciences 10, no. 10 (2023): 623. http://dx.doi.org/10.3390/vetsci10100623.

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Ceruloplasmin (Cp) assessment in biological samples exploits the oxidase activity of this enzyme against several substrates, such as p-phenylenediamine (p-P), o-dianisidine (o-D) and, most recently, ammonium iron(II) sulfate (AIS). Once developed in humans, these assays are often used in veterinary medicine without appropriately optimizing in the animal species of interest. In this study, two assays using AIS and o-D as substrates have been compared and validated for Cp oxidase activity assessment in horse’s plasma. The optimization of the assays was performed mainly by varying the buffer pH a

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43

Huang, Tingting, Guohong Liu, Jingxiang Yu, et al. "A New Portable Colorimetric Sensor Based on RGB Chromaticity for Quantitative Determination of Sarin in Water." Current Analytical Chemistry 16, no. 4 (2020): 475–84. http://dx.doi.org/10.2174/1573411014666181023112032.

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Background: Sarin is a nerve agent which is lethal to people due to its high toxicity. According to its extreme toxicity, sarin, relatively lack of color, highly toxic, miscible in water, poses viable threats to potable water sources. Therefore, there is an urgent need for portable, rapid and yet reliable methods to monitor for adulteration of potable water sources by sarin on spot. Methods: A stock solution of 30 mg/L sarin was prepared daily by dissolving 300 μg of sarin in 10 mL isopropanol. A certain amount of sarin was added to the glass tube, and then o-dianisidine and hydrogen peroxide

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Kloor, Doris, Katrin Karnahl, and Jost Kömpf. "Characterization of glycineN-methyltransferase from rabbit liver." Biochemistry and Cell Biology 82, no. 3 (2004): 369–74. http://dx.doi.org/10.1139/o04-007.

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The enzymatic properties of glycine N-methyltransferase from rabbit liver and the effects of endogenous adenosine nucleosides, nucleotides and methyltransferase inhibitors were investigated using a photometrical assay to detect sarcosine with o-dianisidine as a dye. After isolation and purification the denatured enzyme showed a two-banded pattern by SDS–PAGE. The enzyme was highly specific for its substrates with a pH-optimum at pH 8.6. Glycine N-methyltransferase exhibits Michaelis-Menten kinetics for its substrates, S-adenosylmethionine and glycine, respectively. The apparent Kmand Vmaxvalue

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Larrondo, Luis F., Sergio Lobos, Phillip Stewart, Dan Cullen, and Rafael Vicuña. "Isoenzyme Multiplicity and Characterization of Recombinant Manganese Peroxidases from Ceriporiopsis subvermispora and Phanerochaete chrysosporium." Applied and Environmental Microbiology 67, no. 5 (2001): 2070–75. http://dx.doi.org/10.1128/aem.67.5.2070-2075.2001.

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ABSTRACT We expressed cDNAs coding for manganese peroxidases (MnPs) from the basidiomycetes Ceriporiopsis subvermispora (MnP1) andPhanerochaete chrysosporium (H4) under control of the α-amylase promoter from Aspergillus oryzae inAspergillus nidulans. The recombinant proteins (rMnP1 and rH4) were expressed at similar levels and had molecular masses, both before and after deglycosylation, that were the same as those described for the MnPs isolated from the corresponding parental strains. Isoelectric focusing (IEF) analysis of rH4 revealed several isoforms with pIs between 4.83 and 4.06, and one

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Cordeiro-Santanach, Anna, Sergey V. Prykhozhij, Adam P. Deveau, Kate L. Del Bel, Stuart E. Turvey, and Jason Berman. "A Zebrafish JAK1-A634D Gain-of-Function Model Provides New Insights into the Pathogenesis of Familial Hypereosinophilia." Blood 132, Supplement 1 (2018): 3060. http://dx.doi.org/10.1182/blood-2018-99-112124.

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Abstract Introduction: JAK/STAT signaling is of major importance for hematopoiesis and immune function. A heterozygous JAK1 c.1901C>A de novo point mutation was identified in three members of a family (a mother and two male sons). These patients presented with a unique clinical phenotype of severe atopic dermatitis, markedly elevated peripheral blood eosinophil counts with eosinophilic infiltration of the liver and gastrointestinal tract, hepatosplenomegaly, and failure to thrive. Treatment with ruxolitinib, a JAK1/2 inhibitor, resulted in reduced eosinophilia and improved growth (Del Bel e

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GAZARYAN, G. Irina, L. Natalia KLYACHKO, K. Yulia DULKIS, V. Igor OUPOROV, and V. Andrey LEVASHOV. "Formation and properties of dimeric recombinant horseradish peroxidase in a system of reversed micelles." Biochemical Journal 328, no. 2 (1997): 643–47. http://dx.doi.org/10.1042/bj3280643.

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Wild-type recombinant horseradish peroxidase purified and refolded from Escherichia coli inclusion bodies has been studied in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (Aerosol OT)-reversed micelles in octane. In contrast with native horseradish peroxidase the wild-type recombinant enzyme forms dimeric structures as judged by sedimentation analysis. Peroxidase substrates affect the equilibrium between monomeric and dimeric enzyme forms. The dependence of the catalytic activity of recombinant peroxidase on the degree of hydration of the surfactant exhibits two maxima with pyrog

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Regelsberger, G., C. Jakopitsch, P. G. Furtmüller, et al. "The role of distal tryptophan in the bifunctional activity of catalase-peroxidases." Biochemical Society Transactions 29, no. 2 (2001): 99–105. http://dx.doi.org/10.1042/bst0290099.

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Catalase-peroxidases are bifunctional peroxidases exhibiting an overwhelming catalase activity and a substantial peroxidase activity. Here we present a kinetic study of the formation and reduction of the key intermediate compound I by probing the role of the conserved tryptophan at the distal haem cavity site. Two wild-type proteins and three mutants of Synechocystis catalase-peroxidase (W122A and W122F) and Escherichia coli catalase-peroxidase (W105F) have been investigated by steady-state and stopped-flow spectroscopy. W122F and W122A completely lost their catalase activity whereas in W105F

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Osuji, Akudo Chigozirim, Sabinus Oscar O. Eze, Emmanuel Emeka Osayi, and Ferdinand Chiemeka Chilaka. "Biobleaching of Industrial Important Dyes with Peroxidase Partially Purified from Garlic." Scientific World Journal 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/183163.

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An acidic peroxidase was extracted from garlic (Allium sativum) and was partially purified threefold by ammonium sulphate precipitation, dialysis, and gel filtration chromatography using sephadex G-200. The specific activity of the enzyme increased from 4.89 U/mg after ammonium sulphate precipitation to 25.26 U/mg after gel filtration chromatography. The optimum temperature and pH of the enzyme were 50°C and 5.0, respectively. The Km andVmaxfor H2O2and o-dianisidine were 0.026 mM and 0.8 U/min, and 25 mM and 0.75 U/min, respectively. Peroxidase from garlic was effective in decolourizing Vat Ye

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Basavaiah, Kanakapura, Nagib A. S. Qarah, and Sameer A. M. Abdulrahman. "Application of Cerium (IV) as an Oxidimetric Agent for the Determination of Ethionamide in Pharmaceutical Formulations." Journal of Pharmaceutics 2016 (October 13, 2016): 1–9. http://dx.doi.org/10.1155/2016/5410573.

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Two simple methods are described for the determination of ethionamide (ETM) in bulk drug and tablets using cerium (IV) sulphate as the oxidimetric agent. In both methods, the sample solution is treated with a measured excess of cerium (IV) solution in H2SO4 medium, and after a fixed standing time, the residual oxidant is determined either by back titration with standard iron (II) solution to a ferroin end point in titrimetry or by reacting with o-dianisidine followed by measurement of the absorbance of the orange-red coloured product at 470 nm in spectrophotometry. In titrimetry, the reaction

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