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1

Subramanian, Pramodhini, Namrata K. Bhosale, Joshy M. Easow, Chandni Chandramouli, and K. S. Chenthil. "Ochrobactrum intermedium septicemia." Journal of Infection in Developing Countries 17, no. 11 (2023): 1636–40. http://dx.doi.org/10.3855/jidc.17185.

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Ochrobactrum species are emerging Gram-negative, non-fermenting bacteria with low virulence. Infection with the Ochrobactrum species is commonly nosocomial and has been reported in patients with indwelling medical devices and implants. Among the species of Ochrobactrum infecting humans, Ochrobactrum anthropic and Ochrobactrum intermedium are the commonest ones. We present a case of septicemia caused by Ochrobactrum intermedium in a 75-year-old patient with lower limb cellulitis. This report describes the epidemiology, clinical manifestations, laboratory diagnosis, antibiotic susceptibility pattern, and treatment of Ochrobactrum infections.
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2

Pan, Yao, Shanfa Tang, Wen Ren, et al. "Effect of Electro-Oil Acclimation of an Indigenous Strain on the Performance of Sediment Microbial Fuel Cells (SMFC)." Energies 16, no. 14 (2023): 5582. http://dx.doi.org/10.3390/en16145582.

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Sediment microbial fuel cell (SMFC) is a type of MFC without a proton exchange membrane. However, SMFC have had problems with low-power production performance. In this paper, the effects of native bacteria (K1) in oily sludge and their electro-oil-induced domestication on the power generation and oil removal performance of SMFC were studied. The results showed that K1 belonged to Ochrobactrum intermedium. During the domestication process, an upward trend was shown in the OD600 and ORP values in the culture medium, and it grown best at 0.7 V. Ochrobactrum intermedium K1 significantly increased the average output voltage, electromotive force, and maximum power density of SMFC and reduced the apparent internal resistance of the battery. The maximum power density was 169.43 mW/m3, which was 8.59 times higher than that of the control group. Ochrobactrum intermedium K1 improved the degradation of crude oil by SMFC. Ochrobactrum intermedium K1 enhanced the degradation of high-carbon alkanes and even-carbon alkanes in n-alkanes. Cyclic voltammetry and chronoamperometry tests showed that after acclimation, Ochrobactrum intermedium K1 improved the extracellular electron transfer efficiency (EET) mediated by c-Cyts and flavin by increasing the surface protein redox potential.
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Möller, Lieke V. M., Jan P. Arends, Hermie J. M. Harmsen, Adriaan Talens, Peter Terpstra, and Maarten J. H. Slooff. "Ochrobactrum intermedium Infection after Liver Transplantation." Journal of Clinical Microbiology 37, no. 1 (1999): 241–44. http://dx.doi.org/10.1128/jcm.37.1.241-244.1999.

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A case of bacteremia due to Ochrobactrum intermedium, with concomitant liver abscesses, in an orthotopic liver transplant recipient is presented. Identical microorganisms were isolated from fecal specimens and from an aspirate of a liver abscess that was indicative of invasion of the graft by gastrointestinal spread. 16S DNA sequence analysis of the blood isolate revealed the recovery of the recently proposed new species O. intermedium, closely related to Ochrobactrum anthropi and Brucellaspp.
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Leal-Klevezas, Diana Sara, Octavio Martínez-de-la-Vega, Ector Jaime Ramírez-Barba, Björn Osterman, Juan Pablo Martínez-Soriano, and June Simpson. "Genotyping of Ochrobactrum spp. by AFLP Analysis." Journal of Bacteriology 187, no. 7 (2005): 2537–39. http://dx.doi.org/10.1128/jb.187.7.2537-2539.2005.

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ABSTRACT AFLP was used to analyze the genetic diversity among Ochrobactrum strains. AFLP patterns showed a great genomic variability that separated the samples into three distinct clusters. Ochrobactrum intermedium was found to be closely related to Brucella abortus S99.
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5

Kumar, Kunal, and Prasanna D. Belur. "Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6." Journal of Pure and Applied Microbiology 12, no. 4 (2018): 2327–36. http://dx.doi.org/10.22207/jpam.12.4.75.

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6

Teyssier, Corinne, Hélène Marchandin, Hélène Jean-Pierre, Agnès Masnou, Ghislaine Dusart, and Estelle Jumas-Bilak. "Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples." International Journal of Systematic and Evolutionary Microbiology 57, no. 5 (2007): 1007–13. http://dx.doi.org/10.1099/ijs.0.64416-0.

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Three novel Gram-negative, non-fermenting aerobic bacilli were isolated from human clinical samples. They shared more than 99.8 % of the 16S rRNA gene nucleotide positions. The strains were related to Ochrobactrum intermedium with about 97.48 % 16S rRNA gene sequence similarity. In 16S rRNA gene-, dnaK- and rpoB-based phylogenies, the strains were grouped in a lineage that was distinct from other Ochrobactrum species in the family Brucellaceae. Fatty acid composition, polar lipids, quinone system, DNA–DNA relatedness, genome organization, and physiological and biochemical data differentiated these isolates from recognized species of the genus Ochrobactrum. The three clinical strains therefore represent a novel species within the genus Ochrobactrum, for which the name Ochrobactrum pseudintermedium sp. nov., is proposed. The type strain is ADV31T (=CIP 109116T=DSM 17490T). The DNA G+C content of strain ADV31T was 54.5 mol%.
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7

Kot, Magdalena, Aleksandra Kalińska, Sławomir Jaworski, Mateusz Wierzbicki, Sebastian Smulski, and Marcin Gołębiewski. "In Vitro Studies of Nanoparticles as a Potentially New Antimicrobial Agent for the Prevention and Treatment of Lameness and Digital Dermatitis in Cattle." International Journal of Molecular Sciences 24, no. 7 (2023): 6146. http://dx.doi.org/10.3390/ijms24076146.

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Digital dermatitis (DD) is the second most prevalent disease in dairy cattle. It causes significant losses for dairy breeders and negatively impacts cows’ welfare and milk yield. Despite this, its etiology has not been entirely identified, and available data are limited. Antibiotic therapy is a practical method for managing animal health, but overuse has caused the evolution of antibiotic-resistant bacteria, leading to a loss in antimicrobial efficacy. The antimicrobial properties of metal nanoparticles (NPs) may be a potential alternative to antibiotics. The aim of this study was to determine the biocidal properties of AgNPs, CuNPs, AuNPs, PtNPs, FeNPs, and their nanocomposites against pathogens isolated from cows suffering from hoof diseases, especially DD. The isolated pathogens included Sphingomonas paucimobilis, Ochrobactrum intermedium I, Ochrobactrum intermedium II, Ochrobactrum gallinifaecis, and Actinomyces odontolyticus. Cultures were prepared in aerobic and anaerobic environments. The viability of the pathogens was then determined after applying nanoparticles at various concentrations. The in vitro experiment showed that AgNPs and CuNPs, and their complexes, had the highest biocidal effect on pathogens. The NPs’ biocidal properties and their synergistic effects were confirmed, which may forecast their use in the future treatment and the prevention of lameness in cows, especially DD.
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8

Meletis, Georgios, Pinelopi Amoiridou, Paraskevi Mantzana, Ioanna Soultati, Efthymia Protonotariou, and Lemonia Skoura. "Pneumonia due to Ochrobactrum intermedium in an ICU patient." New Microbes and New Infections 57 (March 2024): 101224. http://dx.doi.org/10.1016/j.nmni.2024.101224.

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9

Imran, A., F. Y. Hafeez, A. Frühling, P. Schumann, K. A. Malik, and E. Stackebrandt. "Ochrobactrum ciceri sp. nov., isolated from nodules of Cicer arietinum." International Journal of Systematic and Evolutionary Microbiology 60, no. 7 (2010): 1548–53. http://dx.doi.org/10.1099/ijs.0.013987-0.

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A Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, Ca-34T, was isolated from nodules of chickpea (Cicer arietinum) in Pakistan and studied for its taxonomic affiliation. The almost full-length 16S rRNA gene sequence showed highest similarities to those of strains of the genus Ochrobactrum. Based on results of MALDI-TOF MS and 16S rRNA gene sequence similarity (98.6 %), strain Ca-34T and Ochrobactrum intermedium LMG 3301T are phylogenetic neighbours; the two strains shared DNA–DNA relatedness of 64 %. The fatty acid profile [predominantly C18 : 1 ω7c (67.7 %) and C19 : 0 cyclo ω8c (19.6 %)] also supported the genus affiliation. Metabolically, strain Ca-34T differed from other type strains of Ochrobactrum in many reactions and from all type strains in testing positive for gelatin hydrolysis and in testing negative for assimilation of alaninamide and l-threonine. Based on phenotypic and genotypic data, we conclude that strain Ca-34T represents a novel species, for which we propose the name Ochrobactrum ciceri sp. nov. (type strain Ca-34T =DSM 22292T =CCUG 57879T).
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10

HIRAI, Jun, Yuka YAMAGISHI, Daisuke SAKANASHI, Yusuke KOIZUMI, Hiroyuki SUEMATSU, and Hiroshige MIKAMO. "A Case of Bacteremia Caused by Ochrobacterium intermedium ." Kansenshogaku Zasshi 90, no. 2 (2016): 129–33. http://dx.doi.org/10.11150/kansenshogakuzasshi.90.129.

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11

Kämpfer, Peter, Bettina Huber, Hans-Jürgen Busse, et al. "Ochrobactrum pecoris sp. nov., isolated from farm animals." International Journal of Systematic and Evolutionary Microbiology 61, no. 9 (2011): 2278–83. http://dx.doi.org/10.1099/ijs.0.027631-0.

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Two Gram-negative, rod-shaped, non-spore-forming strains, designated 08RB2639T and 08RB2781-1, were isolated from a sheep (Ovis aries) and a domestic boar (Sus scrofa domestica), respectively. By 16S rRNA gene sequencing, the isolates revealed identical sequences and were shown to belong to the Alphaproteobacteria. They exhibited 97.8 % 16S rRNA gene sequence similarity with Ochrobactrum rhizosphaerae PR17T, O. pituitosum CCUG 50899T, O. tritici SCII24T and O. haematophilum CCUG 38531T and 97.4 % sequence similarity with O. cytisi ESC1T, O. anthropi LMG 3331T and O. lupini LUP21T. The recA gene sequences of the two isolates showed only minor differences (99.5 % recA sequence similarity), and strain 08RB2639T exhibited the highest recA sequence similarity with Ochrobactrum intermedium CCUG 24694T (91.3 %). The quinone system was ubiquinone Q-10, with minor amounts of Q-9 and Q-11, the major polyamines were spermidine, putrescine and sym-homospermidine and the major lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, with moderate amounts of the Ochrobactrum-specific unidentified aminolipid AL2. The major fatty acids (>20 %) were C18 : 1ω7c and C19 : 0 cyclo ω8c. These traits were in excellent agreement with the assignment of the isolates to the genus Ochrobactrum. DNA–DNA relatedness and physiological and biochemical tests allowed genotypic and phenotypic differentiation from other members of the genus Ochrobactrum. Hence, it is concluded that the isolates represent a novel species, for which the name Ochrobactrum pecoris sp. nov. is proposed (type strain 08RB2639T = DSM 23868T = CCUG 60088T = CCM 7822T).
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12

ISKANDAROVA, Umida, Rustambek ERGASHEV, and Diyorbek QOSIMOV,. "BAKTERIYALARNING FITOPATOGEN ZAMBURUG‘LARGA NISBATAN ANTIFUNGAL FAOLLIGI." UzMU xabarlari 3, no. 3.1 (2025): 36–38. https://doi.org/10.69617/nuuz.v3i3.1.7354.

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Fitopatogen zamburug‘larga qarshi mikrobiologik kurash – zamburug‘lar keltirib chiqaradigan o‘simlik kasalliklarini nazorat qilishning ekologik toza usullaridan biri hisoblanadi. Ushbu maqolada tuproq namunalaridan bakteriyalar ajratib olish, ularning xususiyatlarini o‘rganish hamda ayrim fitopatogen zamburug‘larga nisbatan antifungal xususiyatlarini aniqlash bo‘yicha tadqiqot natijalari keltirilgan. Ajratilgan mikroorganizmlardan Lysinibacillus sphaericus, Ochrobactrum intermedium va Bacillus sp. lar qishloq xo‘jaligi ekinlari uchun xavfli bo‘lgan Fusarium solani, Alternaria sp., Verticillium dahlia kabi fitopatogen zamburug‘larga nisbatan yuqori antoganistik faollik namoyon qildi
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13

Yuan, Y. J., Z. X. Lu, L. J. Huang, et al. "Biodegradation of nicotine from tobacco waste extract by Ochrobactrum intermedium DN2." Journal of Industrial Microbiology & Biotechnology 34, no. 8 (2007): 567–70. http://dx.doi.org/10.1007/s10295-007-0212-x.

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14

Apisarnthanarak, Anucha, Pattarachai Kiratisin, and Linda M. Mundy. "Evaluation of Ochrobactrum intermedium bacteremia in a patient with bladder cancer." Diagnostic Microbiology and Infectious Disease 53, no. 2 (2005): 153–55. http://dx.doi.org/10.1016/j.diagmicrobio.2005.05.014.

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15

Wackerow-Kouzova, N. D. "Ochrobactrum intermedium ANKI, a nitrogen-fixing bacterium able to decolorize azobenzene." Applied Biochemistry and Microbiology 43, no. 4 (2007): 403–6. http://dx.doi.org/10.1134/s0003683807040084.

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16

Teyssier, Corinne, Hélène Marchandin, Michèle Siméon De Buochberg, Michel Ramuz, and Estelle Jumas-Bilak. "Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies." Journal of Bacteriology 185, no. 9 (2003): 2901–9. http://dx.doi.org/10.1128/jb.185.9.2901-2909.2003.

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ABSTRACT Ochrobactrum intermedium is an opportunistic human pathogen belonging to the alpha 2 subgroup of proteobacteria. The 16S rDNA sequences of nine O. intermedium isolates from a collection of clinical and environmental isolates exhibited a 46-bp insertion at position 187, which was present in only one sequence among the 82 complete or partial 16S rDNA sequences of Ochrobactrum spp. available in data banks. Reverse transcription-PCR experiments showed that the 46-bp insertion remained in the 16S rRNA. The inserted sequence folded into a stem-loop structure, which took place in and prolonged helix H184 of the 16S rRNA molecule. Helix H184 has been described as conserved in length among eubacteria, suggesting the idiosyncratic character of the 46-bp insertion. Pulsed-field gel electrophoresis experiments showed that seven of the clinical isolates carrying the 46-bp insertion belonged to the same clone. Insertion and rrn copy numbers were determined by hybridization and I-CeuI digestion. In the set of clonal isolates, the loss of two insertion copies revealed the deletion of a large genomic fragment of 150 kb, which included one rrn copy; deletion occurred during the in vivo evolution of the clone. Determination of the rrn skeleton suggested that the large genomic rearrangement occurred during events involving homologous recombination between rrn copies. The loss of insertion copies suggested a phenomenon of concerted evolution among heterogeneous rrn copies.
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17

Sun, Yumei, Zimeng Zhang, Ling Liu, Xiang Xu, and Xianzhen Li. "Effects of Various Phosphates on Biosurfactant Fermentation by Ochrobactrum Intermedium XY-1." IOP Conference Series: Earth and Environmental Science 706, no. 1 (2021): 012042. http://dx.doi.org/10.1088/1755-1315/706/1/012042.

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18

Sharma, Isha, and Naveen Kango. "Production and characterization of keratinase by Ochrobactrum intermedium for feather keratin utilization." International Journal of Biological Macromolecules 166 (January 2021): 1046–56. http://dx.doi.org/10.1016/j.ijbiomac.2020.10.260.

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19

Yuan, Y. J., Z. X. Lu, L. J. Huang, X. M. Bie, F. X. Lu, and Y. Li. "Optimization of a medium for enhancing nicotine biodegradation by Ochrobactrum intermedium DN2." Journal of Applied Microbiology 101, no. 3 (2006): 691–97. http://dx.doi.org/10.1111/j.1365-2672.2006.02929.x.

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20

Mahendhran, Karthikeyan, Aravinth Arthanari, Baskaran Dheenadayalan, and Murugappan Ramanathan. "Bioconversion of oily bilge waste to polyhydroxybutyrate (PHB) by marine Ochrobactrum intermedium." Bioresource Technology Reports 4 (December 2018): 66–73. http://dx.doi.org/10.1016/j.biteb.2018.08.013.

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21

Monteiro, Joveliane de Melo, Emmanueli Iracema Farah, Rômulo Maia Ferreira, et al. "Identification and antibiotics resistance profile of gram-negative bacteria isolated from sediment Anil river in Maranhão, Brazil." Caderno Pedagógico 22, no. 1 (2025): e13729. https://doi.org/10.54033/cadpedv22n1-277.

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The Manguezal ecosystem suffers impacts from human anthropic actions, which can cause transformations in the microbiota and selection of antibiotic-resistant variants. Thus, this study aimed to isolate and identify gram-negative bacteria to characterize the resistance genes to b-lactam antibiotics from sediment samples from the Anil River – MA (Manguezais area). The bacterial isolate was obtained from sediment samples plated on Macconkey Agar supplemented with antibiotics ceftazidime and meropenem. MALDI-QTOF identified the isolated bacterial species. The bacterial isolates were subjected to the Kirby-Bauer method to evaluate the antibiotic susceptibility profile. The PCR technique detected the genetic determinants. This study identified three and two bacteria; 20 isolates were classified as Ochrobactrum intermedium, 09 Ochrobactrum tritici, 02 Stenotrophomonas maltophilia, and 01 Klebsiella pneumoniae. In antibiotic susceptibility tests, it was observed that 100% (n=32) of the isolates were resistant to cefepime, 96.8% (n=31) to ofloxacin and ceftriaxone, and 90.6% (n=29) to imipenem. The molecular profile showed that 50% (n=16) presented the blaGES gene. In addition, genes belonging to the blaOXA profile were identified in 21 bacteria isolates from the genus Ochrobactrum, with the blaOXA1 gene being the most prevalent among the isolates, identified in 11 bacterial lines. This study confirms that environments such as mangroves, which act as reservoirs of bacteria resistant to multiple antimicrobial drugs for clinical use, become a risk to human health.
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22

Faisal, M., and S. Hasnain. "Growth stimulatory effect of Ochrobactrum intermedium and Bacillus cereus on Vigna radiata plants." Letters in Applied Microbiology 43, no. 4 (2006): 461–66. http://dx.doi.org/10.1111/j.1472-765x.2006.01977.x.

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23

Bezza, Fisseha Andualem, Mervyn Beukes, and Evans M. Nkhalambayausi Chirwa. "Application of biosurfactant produced by Ochrobactrum intermedium CN3 for enhancing petroleum sludge bioremediation." Process Biochemistry 50, no. 11 (2015): 1911–22. http://dx.doi.org/10.1016/j.procbio.2015.07.002.

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24

Yuan, Y. J., Z. X. Lu, N. Wu, L. J. Huang, F. X. Lü, and X. M. Bie. "Isolation and preliminary characterization of a novel nicotine-degrading bacterium, Ochrobactrum intermedium DN2." International Biodeterioration & Biodegradation 56, no. 1 (2005): 45–50. http://dx.doi.org/10.1016/j.ibiod.2005.04.002.

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Yosmaniar, Yosmaniar, Hessy Novita, and Eri Setiadi. "ISOLASI DAN KARAKTERISASI BAKTERI NITRIFIKASI DAN DENITRIFIKASI SEBAGAI KANDIDAT PROBIOTIK." Jurnal Riset Akuakultur 12, no. 4 (2018): 369. http://dx.doi.org/10.15578/jra.12.4.2017.369-378.

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Senyawa nitrogen yang tinggi pada limbah budidaya perikanan intensif dapat memperburuk kualitas air, sehingga perlu diatasi dengan penambahan probiotik untuk proses bioremediasi. Tujuan penelitian ini adalah untuk mendapatkan bakteri nitrifikasi dan denitrifikasi yang berpotensi sebagai kandidat probiotik pengendali senyawa nitrogen pada budidaya ikan air tawar. Tahap penelitian terdiri atas: 1) koleksi sampel air dan sedimen dari kolam budidaya ikan patin di kawasan minapolitan Desa Pudak Kecamatan Kumpeh Kabupaten Muaro Jambi Provinsi Jambi dan Desa Koto Mesjid Kecamatan XIII Koto Kampar Kabupaten Kampar Provinsi Riau; 2) pengujian sampel secara in vitro yang meliputi: a) Isolasi dan seleksi bakteri nitrifikasi dan denitrifikasi; b) Karakterisasi morfologis bakteri terpilih; c) Karakterisasi fisiologi/biokimia isolat bakteri terpilih; d) Karakterisasi genetika isolat bakteri terpilih dengan sekuensing 16S-rRNA. Analisis data dilakukan secara deskriptif. Berdasarkan hasil penelitian diperoleh empat isolat bakteri nitrifikasi dan empat isolate bakteri denitrifikasi. Isolat bakteri nitrifikasi Pandoraea pnomenusa strain 1318 (NP1); Pseudomonas aeruginosa strain PSE12 (NP2); Pseudomonas aeruginosa strain PSE12 (NP3); Burkholderia vietnamiensis strain NE 7 (NP4); dan denitrifikasi Achromobacter xylosoxidans strain TPL14 (DP1); Stenotrophomonas acidaminiphila strain BTY (DP2); Stenotrophomonas maltophilia strain BHWSL2 (DP3); Ochrobactrum intermedium strain: SQ 20 (DP4) Achromobacter xylosoxidans strain TPL14 (DP1); Stenotrophomonas acidaminiphila strain BTY (DP2); Stenotrophomonas maltophilia strain BHWSL2 (DP3); Ochrobactrum intermedium strain: SQ 20 (DP4); yang berpotensi digunakan sebagai kandidat probiotik pengendali senyawa nitrogen pada budidaya ikan air tawar. Wastes from an intensive aquaculture contain nitrogen compounds which, if untreated, could rapidly reduce water quality condition within the system. The addition of probiotics as bioremediation to aquaculture system has been used to improve water quality with promising results. The aim of this study was to obtain potential nitrifying and denitrifying bacteria that could be used as probiotic candidates to control excessive nitrogen compounds in freshwater culture. This study consisted of two steps, 1) the collection of water samples and sediments from catfish ponds at ‘Minapolitan Area” in Pudak Village, Jambi Province and Koto Mesjid Village, Riau Province; 2) in vitro tests consisting of isolation and selection of nitrifying and denitrifying bacteria; morphological characterization of the selected nitrifying and denitrifying bacteria; characterization of physiological/biochemical selected nitrifying and denitrifying bacteria; genetic characterization of the selected nitrifying and denitrifying bacteria with 16SrRNA sequencing. All data were analyzed descriptively. The study had found four nitrifying bacteria isolates: Pandoraea pnomenusa strain 1318 (NP1); Pseudomonas aeruginosa strain PSE 12 (NP2); Pseudomonas aeruginosa strain PSE12 (NP3); Burkholderia vietnamiensis strain NE 7 (NP4). The study also found four isolates of denitrifying bacteria isolates: Achromobacter xylosoxidans strain TPL14 (DP1); Stenotrophomonas acidaminiphila strain BTY (DP2); Stenotrophomonas maltophilia strain BHWSL2 (DP3); Ochrobactrum intermedium strain: SQ 20 (DP4). All the identified nitrifying and denitrifying bacteria isolates have the potential to be used as probiotic candidates to control nitrogen compound in freshwater aquaculture.
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Scholz, Holger Christian, Martin Pfeffer, Angela Witte, et al. "Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene." Journal of Medical Microbiology 57, no. 1 (2008): 64–71. http://dx.doi.org/10.1099/jmm.0.47507-0.

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Yadav, Pooja, Ashutosh Yadav, Janmejai Kumar Srivastava, and Abhay Raj. "Reduction of pollution load of tannery effluent by cell immobilization approach using Ochrobactrum intermedium." Journal of Water Process Engineering 41 (June 2021): 102059. http://dx.doi.org/10.1016/j.jwpe.2021.102059.

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Martínez-Pérez, J. M., D. Robles-Pérez, F. A. Rojo-Vázquez, and M. Martínez-Valladares. "Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica." Research in Veterinary Science 97, no. 2 (2014): 329–32. http://dx.doi.org/10.1016/j.rvsc.2014.07.015.

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Batool, R., and S. Hasnain. "Phytostimulatory effects of chromium reducing ochrobactrum intermedium and Pseudomonas aeruginosa on Triticum aestivum Growth." New Biotechnology 25 (September 2009): S310. http://dx.doi.org/10.1016/j.nbt.2009.06.887.

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30

Martínez-Pérez, J. M., D. Robles-Pérez, F. Valcárcel-Sancho, et al. "Effect of lipopolysaccharide (LPS) from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica." Parasitology Research 112, no. 8 (2013): 2913–23. http://dx.doi.org/10.1007/s00436-013-3463-7.

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Kavita, B., and Haresh Keharia. "Reduction of hexavalent chromium by Ochrobactrum intermedium BCR400 isolated from a chromium-contaminated soil." 3 Biotech 2, no. 1 (2011): 79–87. http://dx.doi.org/10.1007/s13205-011-0038-0.

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32

Zoaiter, Malak, Zaher Zeaiter, Oleg Mediannikov, Cheikh Sokhna, and Pierre-Edouard Fournier. "Carbonyl Cyanide 3-Chloro Phenyl Hydrazone (CCCP) Restores the Colistin Sensitivity in Brucella intermedia." International Journal of Molecular Sciences 24, no. 3 (2023): 2106. http://dx.doi.org/10.3390/ijms24032106.

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Brucella intermedia (formerly Ochrobactrum intermedium), a non-fermentative bacterium, has been isolated from animals and human clinical specimens. It is naturally resistant to polymyxins, including colistin (CO), and may cause opportunistic infections in humans. We isolated six Brucella intermedia strains from Senegalese monkey stool. In order to determine whether an efflux pump mechanism was involved in CO resistance in B. intermedia, we evaluated the effects of verapamil (VRP), reserpine (RSP), phe-arg β-naphthylamide dihydrochloride (PAβN) and carbonyl cyanide 3-chloro phenyl hydrazone (CCCP), four efflux pump inhibitors, on these colistin-resistant strains. Using the broth microdilution method, a CO and CCCP combination of 2 µg/mL and 10 µg/mL, respectively, significantly reduced the CO minimal inhibitory concentration (MIC) of B. intermedia, supporting an efflux pump mechanism. In contrast, VRP, PAβN and RSP did not restore CO susceptibility. A time kill assay showed a bactericidal effect of the CO–CCCP combination. Genomic analysis revealed a potential implication in the CO resistance mechanism of some conserved efflux pumps, such as YejABEF, NorM and EmrAB, as previously reported in other bacteria. An inhibitory effect of the CO–CCCP combination was observed on biofilm formation using the crystal violet method. These results suggest that the intrinsic CO resistance in Brucella intermedia is linked to an efflux pump mechanism and that the synergistic effect of CO–CCCP may open a new field to identify new treatments to restore antibiotic efficacy in humans.
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Asnani, Ari, Annisa Permata Dinda, Abdi Rahman Nursyamsi, and Dwi Utami Anjarwati. "Antimicrobial and Antibiofilm Activities of Bacterial Strain P-6B from Segara Anakan Against MRSA 2983." Molekul 17, no. 1 (2022): 116. http://dx.doi.org/10.20884/1.jm.2022.17.1.5575.

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Methicillin-resistant Staphylococcus aureus (MRSA) infection has become a worldwide concern due to the increasing MRSA resistance to antibiotics. This condition encourages the continuous exploration of new anti-MRSA compounds. Thus, this study explored the effect of carbon (starch, glucose, sucrose, lactose) and nitrogen (casein, yeast extract, urea, peptone) sources for the fermentation medium to produce antibacterial and antibiofilm compounds from bacterial strain P-6B against MRSA 2983. The results showed that six-day incubation produced bioactive extracts with the highest antimicrobial activity (11.361 ± 0.860 mm) and the highest activity to inhibit biofilm formation (89.159 ± 0.293% %). In comparison, three-day incubation produced a bioactive extract with the highest activity to degrade biofilm (86.450 ± 3.260%). Glucose was the best carbon source to produce an extract with antibacterial and biofilm inhibition activities. However, starch was the best carbon source to produce an extract with biofilm degradation activity. Urea was the best nitrogen source to produce extracts with antimicrobial and antibiofilm activities. Species identification based on the 16S rRNA gene revealed a 99.78% similarity with Ochrobactrum intermedium strain NBRC 15820 (NR_113812.1). These findings suggested the potency of Ochrobactrum P-6B from Segara Anakan Cilacap as indigenous sources of bioactive compounds with anti-MRSA activities.
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Wang, Weiqiang, Yang He, Bo Wang, Mei Dong, Haijuan Zhang, and Chen Shen. "Experimental study on wax removal and viscosity reduction of waxy crude oil by Ochrobactrum intermedium." Journal of Petroleum Science and Engineering 213 (June 2022): 110445. http://dx.doi.org/10.1016/j.petrol.2022.110445.

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35

Kulkarni, Girish, Dhiraj Dhotre, Mahesh Dharne, et al. "Draft genome of Ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India." Gut Pathogens 5, no. 1 (2013): 7. http://dx.doi.org/10.1186/1757-4749-5-7.

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36

Kumar, Kunal, and Prasanna D. Belur. "New extracellular thermostable oxalate oxidase produced from endophytic Ochrobactrum intermedium CL6: Purification and biochemical characterization." Preparative Biochemistry & Biotechnology 46, no. 7 (2016): 734–39. http://dx.doi.org/10.1080/10826068.2015.1135458.

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37

Rodríguez-Villodres, Ángel, Ángel Cuevas Palomino, and María José Gómez Gómez. "Usefulness of MALDI-TOF mass spectrometry in infections by infrequent microorganisms such as Ochrobactrum intermedium." Medicina Clínica (English Edition) 147, no. 6 (2016): 277–78. http://dx.doi.org/10.1016/j.medcle.2016.10.011.

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38

Sultan, Sikander, and Shahida Hasnain. "Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals." Bioresource Technology 98, no. 2 (2007): 340–44. http://dx.doi.org/10.1016/j.biortech.2005.12.025.

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39

Rodríguez-Villodres, Ángel, Ángel Cuevas Palomino, and María José Gómez Gómez. "Utilidad de la espectrometría de masas MALDI-TOF en infecciones por microorganismos infrecuentes como Ochrobactrum intermedium." Medicina Clínica 147, no. 6 (2016): 277–78. http://dx.doi.org/10.1016/j.medcli.2016.04.019.

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40

Shukla, Arpit, Paritosh Parmar, Dweipayan Goswami, Baldev Patel, and Meenu Saraf. "Characterization of novel thorium tolerant Ochrobactrum intermedium AM7 in consort with assessing its EPS-Thorium binding." Journal of Hazardous Materials 388 (April 2020): 122047. http://dx.doi.org/10.1016/j.jhazmat.2020.122047.

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41

Gohil, Kushal N., Priya S. Neurgaonkar, Aditi Paranjpe, Syed G. Dastager, and Mahesh S. Dharne. "Peeping into genomic architecture by re-sequencing of Ochrobactrum intermedium M86 strain during laboratory adapted conditions." Genomics Data 8 (June 2016): 72–76. http://dx.doi.org/10.1016/j.gdata.2016.04.003.

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42

Aujoulat, Fabien, Sara Romano-Bertrand, Agnès Masnou, Hélène Marchandin, and Estelle Jumas-Bilak. "Niches, Population Structure and Genome Reduction in Ochrobactrum intermedium: Clues to Technology-Driven Emergence of Pathogens." PLoS ONE 9, no. 1 (2014): e83376. http://dx.doi.org/10.1371/journal.pone.0083376.

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YAMAMOTO, Kazuaki, Takashi TAMURA, Yukitake OKAMURA, Ayako KABAMOTO, Shunei MORIWAKI, and Mariko ETO. "Isolation and Characterization of Ochrobactrum intermedium from Imported Cattle Serologically Diagnosed with Bovine Brucellosis." Journal of the Japan Veterinary Medical Association 63, no. 8 (2010): 615–19. http://dx.doi.org/10.12935/jvma.63.615.

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44

Ong, Zhi-Zhang, Yeak-Heng Chong, and Wey-Lim Wong. "Transient or Permanent Inhabitants: Cultured Internal Microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp." Sains Malaysiana 51, no. 8 (2021): 2339–49. http://dx.doi.org/10.17576/jsm-2022-5108-01.

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Monogeneans, a class of parasitic platyhelminthes, are usually found on the surface of fish. When it feeds on its host, it harbours bacteria, which can survive in its gut. Occasionally, the monogeneans may cause damages on fish tissue while feeding and may allow secondary infection. The present study aimed to isolate and identify culturable bacteria obtained within the gill monogeneans, Cichlidogyrus thurstonaeand Scutogyrus longicornis, and gill surface of the tilapia fish, Oreochromis sp. based on 16S ribosomal RNA gene sequencing. Monogeneans were isolated from the fish gill filaments and surface disinfected using 70% ethanol before squashed aseptically on Luria Bertani (LB) agar to isolate the internal microbiota. A total of five bacteria species, namely Burkholderia sp., Enterobacter hormaechei, Enterobacter sp., Ochrobactrum intermedium and Pantoea sp., were found within C. thurstonae, whilst a total of eight bacteria species, namely Burkholderia contaminans, Pantoea dispersa, Sphingomonas yabuuchiae, Rhizobium pusense, O. intermedium, Acinetobacter bereziniae, Escherichia hermannii, and Staphylococcus saprophyticus, were found within S. longicornis in which B. contaminans, P. dispersa, and S. yabuuchiaewere also found on the surface of the tilapia fish gill filaments. Enterobacter bugandensisand Acinetobacter pittiiwere found solely on the surface of the gill filaments. These bacteria are also found in the environment and some of them are believed to be pathogenic to fish. We suggest that monogeneans may serve as potential bacteria reservoirs, which facilitate the transmission of bacteria.
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Sharma, Babita, and Pratyoosh Shukla. "A comparative analysis of heavy metal bioaccumulation and functional gene annotation towards multiple metal resistant potential by Ochrobactrum intermedium BPS-20 and Ochrobactrum ciceri BPS-26." Bioresource Technology 320 (January 2021): 124330. http://dx.doi.org/10.1016/j.biortech.2020.124330.

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Kumar, Kunal, and Prasanna Devarabhat. "Chemical Modification of Oxalate Oxidase Produced from Ochrobactrum intermedium CL6 Gave New Insight on its Catalytic Prowess." Asian Journal of Biochemistry 12, no. 1 (2016): 9–15. http://dx.doi.org/10.3923/ajb.2017.9.15.

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Faisal, Muhammad, and Shahida Hasnain . "Colonization Potential of Ochrobactrum intermedium, Bacillus cereus and Brevibacterium sp. on Triticum aestivum and Helianthus annuus Roots." Journal of Plant Sciences 1, no. 1 (2005): 36–41. http://dx.doi.org/10.3923/jps.2006.36.41.

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Faisal, Muhammad, and Shahida Hasnain. "Colonization Potential of Ochrobactrum intermedium, Bacillus cereus and Brevibacterium sp. on Triticum aestivum and Helianthus annuus Roots*." Journal of Plant Sciences 5, no. 1 (2009): 55–60. http://dx.doi.org/10.3923/jps.2010.55.60.

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Riaz, Saima, Muhammad Faisal, and Shahida Hasnain. "Cicer arietinum growth promotion by Ochrobactrum intermedium and Bacillus cereus in the presence of CrCl3 and K2CrO4." Annals of Microbiology 60, no. 4 (2010): 729–33. http://dx.doi.org/10.1007/s13213-010-0123-3.

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50

Sultan, Sikander, and Shahida Hasnain. "Characterization of an Ochrobactrum intermedium strain STCr-5 manifesting high level Cr(VI) resistance and reduction potential." Enzyme and Microbial Technology 39, no. 4 (2006): 883–88. http://dx.doi.org/10.1016/j.enzmictec.2006.01.019.

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