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Journal articles on the topic 'Off-target assessment'

Author: Grafiati

Published: 11 March 2023

Last updated: 31 July 2025

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1

Soltani, Nader, Maxwel C. Oliveira, Guilherme S. Alves, et al. "Off-target movement assessment of dicamba in North America." Weed Technology 34, no. 3 (2020): 318–30. http://dx.doi.org/10.1017/wet.2020.17.

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AbstractSix experiments were conducted in 2018 on field sites located in Arkansas, Indiana, Michigan, Nebraska, Ontario, and Wisconsin to evaluate the off-target movement (OTM) of dicamba under field-scale conditions. The highest estimated percentages of dicamba injury in non–dicamba-resistant (DR) soybean were 55%, 44%, 39%, 67%, 15%, and 44% injury for noncovered areas and 55%, 5%, 13%, 42%, 0%, and 41% injury for covered areas during dicamba application in Arkansas, Indiana, Michigan, Nebraska, Ontario, and Wisconsin, respectively. The level of injury generally decreased as the downwind dis

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Wang, Dan, Cuili Niu, Jingxin Han, Dejun Ma, and Zhen Xi. "Target DNA mutagenesis-based fluorescence assessment of off-target activity of the CRISPR-Cas9 system." RSC Advances 9, no. 16 (2019): 9067–74. http://dx.doi.org/10.1039/c8ra10017a.

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Chaudhari, Hemangi G., Jon Penterman, Holly J. Whitton, et al. "Evaluation of Homology-Independent CRISPR-Cas9 Off-Target Assessment Methods." CRISPR Journal 3, no. 6 (2020): 440–53. http://dx.doi.org/10.1089/crispr.2020.0053.

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Ratcliffe, Laura, Sophie Vermond, Sophie Hill, et al. "Abstract LB009: Safety profile assessment for IND-enabling studies: Bispecific antibodies and antibody-drug conjugates (ADCs)." Cancer Research 85, no. 8_Supplement_2 (2025): LB009. https://doi.org/10.1158/1538-7445.am2025-lb009.

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Abstract Developing antibody-based therapeutics requires balancing potency and safety. Off-target effects can cause adverse events, necessitating precise target engagement and comprehensive safety profiling during early development. The work presented outlines a systematic approach to identify and mitigate off-target interactions for antibodies and ADCs. This de-risking roadmap which spans 12-16 weeks, evaluates on-target and off-target interactions, ensuring thorough vetting before IND-enabling studies. The de-risking platform uses two primary approaches: (1) Off-target analysis via Retrogeni

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Corsi, Giulia I., Veerendra P. Gadekar, Jan Gorodkin, and Stefan E. Seemann. "CRISPRroots: on- and off-target assessment of RNA-seq data in CRISPR–Cas9 edited cells." Nucleic Acids Research 50, no. 4 (2021): e20-e20. http://dx.doi.org/10.1093/nar/gkab1131.

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Abstract The CRISPR-Cas9 genome editing tool is used to study genomic variants and gene knockouts, and can be combined with transcriptomic analyses to measure the effects of such alterations on gene expression. But how can one be sure that differential gene expression is due to a successful intended edit and not to an off-target event, without performing an often resource-demanding genome-wide sequencing of the edited cell or strain? To address this question we developed CRISPRroots: CRISPR–Cas9-mediated edits with accompanying RNA-seq data assessed for on-target and off-target sites. Our meth

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Hernández, José Esteban. "Acquisition of The Tap-Trill-Lateral Contrast by Speakers of L1 Cantonese-L2 Spanish." Revista de Filología y Lingüística de la Universidad de Costa Rica 51, no. 1 (2025): e63517. https://doi.org/10.15517/rfl.v51i1.63517.

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In the present study an assessment of the on-target and off-target production of liquids (laterals and rhotics) is undertaken in a corpus of second language (L2) Spanish. The Chinese-origin participants have immigrated to Mexicali, Mexico where they have acquired L2 Spanish in a natural setting. As a major aim, a quantitative analysis of the data is performed to determine the production of on-target and off-target articulations in lateral, tap, thrill, and syllable-coda rhotic. Internal and external factors are considered in the analysis; for example, liquid production in syllable onset, coda

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Fischer, André, Manuel Sellner, Karolina Mitusińska, et al. "Computational Selectivity Assessment of Protease Inhibitors against SARS-CoV-2." International Journal of Molecular Sciences 22, no. 4 (2021): 2065. http://dx.doi.org/10.3390/ijms22042065.

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The pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a serious global health threat. Since no specific therapeutics are available, researchers around the world screened compounds to inhibit various molecular targets of SARS-CoV-2 including its main protease (Mpro) essential for viral replication. Due to the high urgency of these discovery efforts, off-target binding, which is one of the major reasons for drug-induced toxicity and safety-related drug attrition, was neglected. Here, we used molecular docking, toxicity profiling, and multiple molecular dynamics (

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Zhao, Hui, and Jeffrey D. Wolt. "Risk associated with off-target plant genome editing and methods for its limitation." Emerging Topics in Life Sciences 1, no. 2 (2017): 231–40. http://dx.doi.org/10.1042/etls20170037.

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Assessment for potential adverse effects of plant genome editing logically focuses on the specific characteristics of the derived phenotype and its release environment. Genome-edited crops, depending on the editing objective, can be classified as either indistinguishable from crops developed through conventional plant breeding or as crops which are transgenic. Therefore, existing regulatory regimes and risk assessment procedures accommodate genome-edited crops. The ability for regulators and the public to accept a product focus in the evaluation of genome-edited crops will depend on research w

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Zhang, Weiwei, Jianhang Yin, Zhengrong Zhang-Ding, et al. "In-depth assessment of the PAM compatibility and editing activities of Cas9 variants." Nucleic Acids Research 49, no. 15 (2021): 8785–95. http://dx.doi.org/10.1093/nar/gkab507.

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Abstract A series of Cas9 variants have been developed to improve the editing fidelity or targeting range of CRISPR–Cas9. Here, we employ a high-throughput sequencing approach primer-extension-mediated sequencing to analyze the editing efficiency, specificity and protospacer adjacent motif (PAM) compatibility of a dozen of SpCas9 variants at multiple target sites in depth, and our findings validate the high fidelity or broad editing range of these SpCas9 variants. With regard to the PAM-flexible SpCas9 variants, we detect significantly increased levels of off-target activity and propose a trad

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Conroy, A., E. Vilchez, D. Nguyen, et al. "AMPLIFYBIO TCR SPECIFICITY PLATFORM: BETTER OFF-TARGET ASSESSMENT FOR SAFER TCR-BASED THERAPIES." Cytotherapy 26, no. 6 (2024): S187. http://dx.doi.org/10.1016/j.jcyt.2024.03.371.

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Yuan, Shixing. "Mitigating the Off-target Effects in CRISPR/Cas9-mediated Genetic Editing with Bioinformatic Technologies." Transactions on Materials, Biotechnology and Life Sciences 3 (March 24, 2024): 318–26. http://dx.doi.org/10.62051/dpgwbz03.

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The biological and clinical fields have recognized the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated protein 9) system as a precise and efficient tool for editing the genome. Despite its merits, the system poses crucial challenges, notably the off-target effects which could lead to unintended mutations - a substantial impediment for clinical applications that may potentially compromise the validity of research and the safety of therapeutic applications. Bioinformatics plays a pivotal role in mitigating this risk. Utilizing more refined bioinformatic to

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Ivanov, P. Yu, and O. G. Mikhailova. "By-catch in the specialized fishery of target crab species near Kamchatka." Researches of the aquatic biological resources of Kamchatka and the North-West Part of the Pacific Ocean 2, no. 64 (2022): 35–54. http://dx.doi.org/10.15853/2072-8212.2022.64.35-54.

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Results of the assessment of by-catch in the specialized fishery of target crab species are presented. The studies covered three fishing areas: off Western Kamchatka (Kamchatka-Kuril and West Kamchatka sub- zones) and off the southeastern coast of Kamchatka (Petropavlovsk-Komandorskaya subzone). In addition to the target object, the catches included both non-target species of crabs and representatives of other groups of animals: fish and invertebrates. The by-catch of fish included representatives of several genera, the most common being Pacific cod (Gadus macrocephalus), Irish lords (Hemilepi

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Wang, Jingbo. "Machine learning-based risk assessment of gene editing: degeneration during gene editing possibility of target effect." Theoretical and Natural Science 82, no. 1 (2025): 33–38. https://doi.org/10.54254/2753-8818/2024.ka20158.

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CRISPI-Cas 9 technology is currently the most popular gene editing tool, which has been used in biomedical research and clinical treatment, but it also has the problem of off-target effects that cause unexpected gene mutations, which limits its safety and efficacy. Now, various machine learning models have been trained to predict off-target events in order to: Gene editing is more precise. In terms of both accuracy and interpretability there are still issues in the existing models. This paper has focused on reviewing Machine learning archery Off Target Prediction models and their performance.

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Jefferts, Katharine, Janusz Burczynski, and William G. Pearcy. "Acoustical Assessment of Squid (Loligo opalescens) off the Central Oregon Coast." Canadian Journal of Fisheries and Aquatic Sciences 44, no. 6 (1987): 1261–67. http://dx.doi.org/10.1139/f87-149.

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Squid (Loligo opalescens) were surveyed acoustically in an area off the Oregon coast using dual-beam and echo integrator techniques. The method developed is shown to be feasible for in situ estimation of target strength, distribution, and abundance of midwater squids. Average target strength was estimated both while drifting with a squid school and cruising over one. The values derived, −58.6 to −58.7 dB, are much lower than previous estimates, but more realistic in relation to the target strength of fishes. Schools judged to be fish or squid showed little difference in depth or size, but vari

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Lim, Ming Tsuey, Norazida Ab Rahman, Xin Rou Teh, et al. "Optimal cut-off points for adherence measure among patients with type 2 diabetes in primary care clinics: a retrospective analysis." Therapeutic Advances in Chronic Disease 12 (January 2021): 204062232199026. http://dx.doi.org/10.1177/2040622321990264.

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Background: Medication adherence measures are often dichotomized to classify patients into those with good or poor adherence using a cut-off value ⩾80%, but this cut-off may not be universal across diseases or medication classes. This study aimed to examine the cut-off value that optimally distinguish good and poor adherence by using the medication possession ratio (MPR) and proportion of days covered (PDC) as adherence measures and glycated hemoglobin (HbA1c) as outcome measure among type 2 diabetes mellitus (T2DM) patients. Method: We used pharmacy dispensing data of 1461 eligible T2DM patie

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Lin, Linda Yingqi, Samuele Cancellieri, Jing Zeng, et al. "Human Genetic Diversity Alters Therapeutic Gene Editing Off-Target Outcomes." Blood 138, Supplement 1 (2021): 3993. http://dx.doi.org/10.1182/blood-2021-152429.

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Abstract CRISPR gene editing holds great promise to modify somatic genomes to ameliorate disease. In silico prediction of homologous sites coupled with biochemical evaluation of possible genomic off-targets may predict genotoxicity risk of individual gene editing reagents. However, standard computational and biochemical methods focus on reference genomes and do not consider the impact of genetic diversity on off-target potential. Here we developed a web application called CRISPRme that explicitly and efficiently integrates human genetic variant datasets with orthogonal genomic annotations to p

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Rao, D. D., N. Senzer, M. A. Cleary, and J. Nemunaitis. "Comparative assessment of siRNA and shRNA off target effects: what is slowing clinical development." Cancer Gene Therapy 16, no. 11 (2009): 807–9. http://dx.doi.org/10.1038/cgt.2009.53.

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Garrood, William T., Nace Kranjc, Karl Petri, et al. "Analysis of off-target effects in CRISPR-based gene drives in the human malaria mosquito." Proceedings of the National Academy of Sciences 118, no. 22 (2021): e2004838117. http://dx.doi.org/10.1073/pnas.2004838117.

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CRISPR-Cas9 nuclease-based gene drives have been developed toward the aim of control of the human malaria vector Anopheles gambiae. Gene drives are based on an active source of Cas9 nuclease in the germline that promotes super-Mendelian inheritance of the transgene by homology-directed repair (“homing”). Understanding whether CRISPR-induced off-target mutations are generated in Anopheles mosquitoes is an important aspect of risk assessment before any potential field release of this technology. We compared the frequencies and the propensity of off-target events to occur in four different gene-d

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Naeem, Muhammad, and Omer S. Alkhnbashi. "Current Bioinformatics Tools to Optimize CRISPR/Cas9 Experiments to Reduce Off-Target Effects." International Journal of Molecular Sciences 24, no. 7 (2023): 6261. http://dx.doi.org/10.3390/ijms24076261.

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The CRISPR-Cas system has evolved into a cutting-edge technology that has transformed the field of biological sciences through precise genetic manipulation. CRISPR/Cas9 nuclease is evolving into a revolutionizing method to edit any gene of any species with desirable outcomes. The swift advancement of CRISPR-Cas technology is reflected in an ever-expanding ecosystem of bioinformatics tools designed to make CRISPR/Cas9 experiments easier. To assist researchers with efficient guide RNA designs with fewer off-target effects, nuclease target site selection, and experimental validation, bioinformati

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He-Hua, Wang, Zhu Jian-Jun, Gu Xiao-Yan, and Chen Ye. "A Multiple Criteria Decision Analysis Method for Alternative Assessment Results Obeying a Particular Distribution and Application." Mathematical Problems in Engineering 2018 (2018): 1–14. http://dx.doi.org/10.1155/2018/2108726.

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A multiple criteria decision analysis (MCDA) problem is studied in this paper, for which the evaluation results obey a particular distribution. First, when solving a multiple criteria decision analysis (MCDA) problem, a grey target decision analysis framework is proposed to determine uncertain parameters and criteria weights. A measurement for comprehensive off-target distance is defined, which includes the undetermined parameters. Second, to satisfy the requirements of a specific distribution (such as a normal distribution) in the assessment results, an optimization model that incorporates th

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Norden, Diana, Carmen Navia, Tabb Sullivan, Alexander Hobby, and Benjamin Doranz. "Abstract 1161: A systematic evaluation of off-target binding in antibody-based biologics during clinical and preclinical development." Cancer Research 85, no. 8_Supplement_1 (2025): 1161. https://doi.org/10.1158/1538-7445.am2025-1161.

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Abstract Background: Specificity profiling is a requirement for monoclonal antibodies (MAbs) and antibody-based biotherapeutics such as CAR-T prior to initiating human trials. Traditional approaches to assess MAb specificity, primarily tissue cross-reactivity (TCR) studies, have been unreliable and have resulted in undetected off-target binding. Cell-based protein arrays represent an alternative and improved assessment tool for MAb specificity that have been recommended in recent FDA guidance. Methods: We developed a cell-based protein array, the Membrane Proteome Array (MPA), to assess bindin

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Hall, F. R., J. Cooper, L. Kirchner, R. Downer, and R. Thacker. "Assessment of off-target movement of orchard pesticides: Capture efficiencies of synthetic and biological biomarkers." Journal of Environmental Science and Health, Part B 31, no. 4 (1996): 815–30. http://dx.doi.org/10.1080/03601239609373038.

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Bethke, Maria, Pierre Abramowski, Miriam Droste, et al. "Identification and Characterization of Fully Human FOLR1-Targeting CAR T Cells for the Treatment of Ovarian Cancer." Cells 13, no. 22 (2024): 1880. http://dx.doi.org/10.3390/cells13221880.

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CAR T cell therapy has been an effective treatment option for hematological malignancies. However, the therapeutic potential of CAR T cells can be reduced by several constraints, partly due to immunogenicity and toxicities. The lack of established workflows enabling thorough evaluation of new candidates, limits comprehensive CAR assessment. To improve the selection of lead CAR candidates, we established a stringent, multistep workflow based on specificity assessments, employing multiple assays and technologies. Moreover, we characterized a human FOLR1-directed CAR binding domain. Selection of

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Fan, Cuiqing, Xiongwei Cai, Feng Zhang, et al. "Precision Assessment of on- and Off-Target Effects of mTOR Kinase Inhibitors in a Mouse Model." Blood 132, Supplement 1 (2018): 2632. http://dx.doi.org/10.1182/blood-2018-99-115225.

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Abstract mTOR plays a critical role in controlling cell growth, survival and metabolism and is an important anti-cancer target. There are over 2,000 clinical trials involving mTOR inhibitors as single or combo-agent. The first gen mTOR inhibitors, i.e. rapamycin and rapalogs, are only effective towards mTORC1 and have shown limited efficacy in multiple clinical settings. The second gen mTOR inhibitors target mTOR kinase domain with significantly stronger antineoplastic potency, and over 30 clinical trials of various mTOR kinase inhibitors including AZD2014 are under way. While the new mTOR inh

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Ahmad, Fiaz, Songchao Zhang, Baijing Qiu, et al. "Comparison of Water Sensitive Paper and Glass Strip Sampling Approaches to Access Spray Deposit by UAV Sprayers." Agronomy 12, no. 6 (2022): 1302. http://dx.doi.org/10.3390/agronomy12061302.

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Target and off-target spray depositions determine the spray’s effectiveness and impact on the environment. A decisive stage in the measurement of spray deposition and drift is selecting an appropriate sampling approach under field conditions. There are various approaches available for sampling spray deposition and drift, during the evaluation of ground sprayers used for the UAV sprayer assessment, under field conditions. In this study, two sampling approaches (water-sensitive paper, and glass strip collectors) were compared to analyze spray deposition in target and off-target zones. The result

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Russo, Monia Teresa, Riccardo Aiese Cigliano, Walter Sanseverino, and Maria Immacolata Ferrante. "Assessment of genomic changes in a CRISPR/Cas9 Phaeodactylum tricornutum mutant through whole genome resequencing." PeerJ 6 (October 5, 2018): e5507. http://dx.doi.org/10.7717/peerj.5507.

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The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, co-opted from a bacterial defense natural mechanism, is the cutting edge technology to carry out genome editing in a revolutionary fashion. It has been shown to work in many different model organisms, from human to microbes, including two diatom species, Phaeodactylum tricornutum and Thalassiosira pseudonana. Transforming P. tricornutum by bacterial conjugation, we have performed CRISPR/Cas9-based mutagenesis delivering the nuclease as an episome; this allowed for avoiding unwanted perturbations due to random in

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Feng, Joseph C., Dean G. Thompson, and Philip E. Reynolds. "Fate of glyphosate in a Canadian forest watershed. 1. Aquatic residues and off-target deposit assessment." Journal of Agricultural and Food Chemistry 38, no. 4 (1990): 1110–18. http://dx.doi.org/10.1021/jf00094a045.

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Bae, Taegeun, Hanseop Kim, Jeong Hee Kim, et al. "Specificity Assessment of CRISPR Genome Editing of Oncogenic EGFR Point Mutation with Single-Base Differences." Molecules 25, no. 1 (2019): 52. http://dx.doi.org/10.3390/molecules25010052.

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In CRISPR genome editing, CRISPR proteins form ribonucleoprotein complexes with guide RNAs to bind and cleave the target DNAs with complete sequence complementarity. CRISPR genome editing has a high potential for use in precision gene therapy for various diseases, including cancer and genetic disorders, which are caused by DNA mutations within the genome. However, several studies have shown that targeting the DNA via sequence complementarity is imperfect and subject to unintended genome editing of other genomic loci with similar sequences. These off-target problems pose critical safety issues

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Mogren, Christina L., and Jonathan Gary Lundgren. "In silico identification of off-target pesticidal dsRNA binding in honey bees (Apis mellifera)." PeerJ 5 (December 13, 2017): e4131. http://dx.doi.org/10.7717/peerj.4131.

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Background Pesticidal RNAs that silence critical gene function have great potential in pest management, but the benefits of this technology must be weighed against non-target organism risks. Methods Published studies that developed pesticidal double stranded RNAs (dsRNAs) were collated into a database. The target gene sequences for these pesticidal RNAs were determined, and the degree of similarity with sequences in the honey bee genome were evaluated statistically. Results We identified 101 insecticidal RNAs sharing high sequence similarity with genomic regions in honey bees. The likelihood t

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Pimentel, Helly, Helen Jarnagin, Hailing Zong, et al. "Preclinical CAR-T cell target safety, biodistribution, and tumor infiltration analysis using in situ hybridization technology." Journal of Clinical Oncology 37, no. 8_suppl (2019): 112. http://dx.doi.org/10.1200/jco.2019.37.8_suppl.112.

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112 Background: Chimeric antigen receptor (CAR) T cell therapy is highly effective in treating hematologic malignancies, and major efforts are being made to achieve similar efficacy in solid tumors. The greater potency of CAR-T cells compared to antibody therapeutics demands a more stringent CAR-T target safety assessment to avoid adverse events resulting from “on-target/off-tumor” activity. Furthermore, it is critical to track and monitor CAR+ T cells within intact tissue and tumor to understand the mechanisms underlying off-tumor toxicity and efficacy in tumor killing. Methods: We employed t

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Bulgarella, Mariana, Aiden Reason, James W. Baty, et al. "In Silico Analysis of Potential Off-Target Effects of a Next-Generation dsRNA Acaricide for Varroa Mites (Varroa destructor) and Lack of Effect on a Bee-Associated Arthropod." Insects 16, no. 3 (2025): 317. https://doi.org/10.3390/insects16030317.

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Double-stranded RNA (dsRNA) biopesticides offer the potential for highly targeted pest control with minimal off-target impacts. Varroa mites (Varroa destructor) are an important pest of honey bees (Apis mellifera) that are primarily managed by synthetic pesticides. A next-generation treatment using a varroa-active dsRNA, vadescana, has been developed to target calmodulin expression in varroa. We evaluated the potential exposure of non-target species to vadescana. First, we assessed potential gene silencing effects on 39 arthropods with known genomes via bioinformatics. Three mite species, mona

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Skadiņa, Dēna, Ināra Nokalna, and Alise Balcere. "Assessment of Micellar Water pH and Product Claims." Dermato 4, no. 3 (2024): 79–85. http://dx.doi.org/10.3390/dermato4030009.

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Micellar waters are widely used skincare cleansing products. It is commonly considered that micellar waters do not need to be rinsed off. Products left on the skin can affect its pH, which typically ranges from 4.1 to 5.8. and plays a vital role in maintaining the integrity of the skin barrier. Our objective was to evaluate the pH of micellar waters and investigate product claims, and differences according to target skin type. The pH of 30 samples of different micellar waters was tested. The products were categorized into groups based on target skin type. Statistical analysis was performed on

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Vaillant, Andrew. "Oligonucleotide-Based Therapies for Chronic HBV Infection: A Primer on Biochemistry, Mechanisms and Antiviral Effects." Viruses 14, no. 9 (2022): 2052. http://dx.doi.org/10.3390/v14092052.

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Three types of oligonucleotide-based medicines are under clinical development for the treatment of chronic HBV infection. Antisense oligonucleotides (ASOs) and synthetic interfering RNA (siRNA) are designed to degrade HBV mRNA, and nucleic acid polymers (NAPs) stop the assembly and secretion of HBV subviral particles. Extensive clinical development of ASOs and siRNA for a variety of liver diseases has established a solid understanding of their pharmacodynamics, accumulation in different tissue types in the liver, pharmacological effects, off-target effects and how chemical modifications and de

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Patel, Bhavinkumar B., Carlos A. Barrero, Alan Braverman, et al. "Assessment of Two Immunodepletion Methods: Off-Target Effects and Variations in Immunodepletion Efficiency May Confound Plasma Proteomics." Journal of Proteome Research 11, no. 12 (2012): 5947–58. http://dx.doi.org/10.1021/pr300686k.

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Ahmed, Firoz, Muthappa Senthil-Kumar, Xinbin Dai, et al. "pssRNAit: A Web Server for Designing Effective and Specific Plant siRNAs with Genome-Wide Off-Target Assessment." Plant Physiology 184, no. 1 (2020): 65–81. http://dx.doi.org/10.1104/pp.20.00293.

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Cancellieri, Samuele, Matthew C. Canver, Nicola Bombieri, Rosalba Giugno, and Luca Pinello. "CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification for CRISPR genome editing." Bioinformatics 36, no. 7 (2019): 2001–8. http://dx.doi.org/10.1093/bioinformatics/btz867.

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ABSTRACT Motivation Clustered regularly interspaced short palindromic repeats (CRISPR) technologies allow for facile genomic modification in a site-specific manner. A key step in this process is the in silico design of single guide RNAs to efficiently and specifically target a site of interest. To this end, it is necessary to enumerate all potential off-target sites within a given genome that could be inadvertently altered by nuclease-mediated cleavage. Currently available software for this task is limited by computational efficiency, variant support or annotation, and assessment of the functi

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Raghavan, Kristopher S., Jonathan T. Sullivan, Carmen Navia, Rachel H. Fong, and Benjamin J. Doranz. "Abstract 5798: Preclinical safety assessment for specificity of biotherapeutics using the membrane proteome array." Cancer Research 84, no. 6_Supplement (2024): 5798. http://dx.doi.org/10.1158/1538-7445.am2024-5798.

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Abstract Rigorous specificity analysis is critical for the development of antibody-based therapies, as even minimal off-target binding can lead to toxicity and clinical failures. Long believed to be exquisitely specific, recent preclinical data and our own work indicate that monoclonal antibodies (MAbs) frequently (~25%) display cross-reactivity. In many cases, off-target interactions occur with unrelated proteins that cannot be predicted by protein sequence homology. Cross-reactivity can lead to serious or even life-threatening consequences especially when MAbs are configured as bispecifics,

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Khaksar, Abbas, Ahmadreza Younessi, and Inyau Bada. "Sanding evaluation and real-time sand management decisions in high-rate gas wells, Ichthys Field, Browse Basin Australia." APPEA Journal 63, no. 2 (2023): S198—S203. http://dx.doi.org/10.1071/aj22180.

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This paper demonstrates the workflow of an integrated petrophysical, geomechanical sanding assessment and cement bond log evaluation to define a simple cut-off for reliable sand production assessments and well completion decisions. This workflow provides valuable insights for timely and fit-for-purpose sand management strategies particularly at early field life where the commonly used sanding prediction methodologies cannot be calibrated with field data because of the lack of sanding observations. The successful application of this methodology is shown in the Ichthys gas field, Browse Basin, w

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Casolaro, P., V. Izzo, R. Vari, et al. "TID damage assessment on LVDS links for the ATLAS muon barrel spectrometer readout system." Journal of Instrumentation 20, no. 01 (2025): C01023. https://doi.org/10.1088/1748-0221/20/01/c01023.

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Abstract This work reports on Total Ionizing Dose (TID) assessment on off-the-shelf LVDS links that will be used for the new trigger and readout system of the ATLAS muon barrel spectrometer within the HL-LHC program. We developed an experimental setup that allows to investigate TID effects on the currents drawn by the devices under test and on their signal integrity, including variations in amplitude, rise/fall time, and bit error rate. No significant variations of these quantities has been observed after integrating the ATLAS target dose of 84.6 Gy. In order to investigate high-dose effects,

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Amatya, Shirisha, Jesse Green, Chi-Shuen Chu, et al. "Enabling Potent T Cell Delivery and Mitigating Key Off-Target Concerns of In Vivo CAR T Lentiviral Vectors with the Targeted Paramyxovirus Fusogen System." Blood 144, Supplement 1 (2024): 2047. https://doi.org/10.1182/blood-2024-211267.

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The promise of in vivo CAR T is to achieve or exceed the efficacy of ex vivo CAR Ts with a systemically delivered vector, enabling off-the-shelf CAR T therapy without lymphodepletion. Ex vivo approaches generally incorporate cell enrichment or selection steps so that only T cells are modified and delivered to the patient. Accordingly the burden for in vivo approaches will be to limit off-target (non-T cell) delivery and mitigate any consequent risk of CAR delivery to non-T cells. Different approaches have been used to engineer vectors that can mediate cell-specific delivery, including lentivir

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Goldmacher, Gregory V., Anuradha D. Khilnani, Robert H. I. Andtbacka, et al. "Response criteria for intratumoral immunotherapy in solid tumors: ItRECIST." Journal of Clinical Oncology 38, no. 15_suppl (2020): 3141. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.3141.

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3141 Background: The approval of intratumoral (IT) immunotherapy for metastatic melanoma and the active development of numerous novel IT drugs have created a need for standardized evaluation of response to this unique treatment strategy. The Response Evaluation Criteria in Solid Tumors (RECIST) is not suitable for assessing responses separately for injected and noninjected tumors. Building on RECIST concepts, we propose an IT immunotherapy RECIST (itRECIST) to capture data and assess local and systemic responses in a standardized fashion for clinical trials involving IT immunotherapies. Method

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Fennell, Myles, Qing Xiang, Alexia Hwang, et al. "Impact of RNA-Guided Technologies for Target Identification and Deconvolution." Journal of Biomolecular Screening 19, no. 10 (2014): 1327–37. http://dx.doi.org/10.1177/1087057114548414.

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For well over a decade, RNA interference (RNAi) has provided a powerful tool for investigators to query specific gene targets in an easily modulated loss-of-function setting, both in vitro and in vivo. Hundreds of publications have demonstrated the utility of RNAi in arrayed and pooled-based formats, in a wide variety of cell-based systems, including clonal, stem, transformed, and primary cells. Over the years, there have been significant improvements in the design of target-specific small-interfering RNA (siRNA) and short-hairpin RNA (shRNA), expression vectors, methods for mitigating off-tar

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Yakovleva, I. V., and A. M. Kamionskaya. "Using New Bioinformatics Strategies at the Design Stage of Genome-edited Plants." Прикладная биохимия и микробиология 59, no. 6 (2023): 525–37. http://dx.doi.org/10.31857/s0555109923060211.

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The identification of risks associated with novel agricultural products of plant origin obtained via genome editing is an important aspect of genetic engineering. An extensive discussion is currently ongoing worldwide to clarify the similarities and differences between the “old” risks of “classic” GM plants and the “new” ones associated with genome editing, the lack of existing methods for identification and assessment of new risks. We propose here the concept of “safe by design” as applied to protection that is a new interesting tool that introduces good known standards of safety into plant b

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Bashir, Fidal I., Richard T. J. Porter, Elena Catalanotti, and Haroun Mahgerefteh. "Performance and Cost Analysis of Pressure Swing Adsorption for Recovery of H2, CO, and CO2 from Steelworks Off-Gases." Energies 18, no. 10 (2025): 2440. https://doi.org/10.3390/en18102440.

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The conceptual design and techno-economic assessment of Pressure Swing Adsorption (PSA) for the recovery of H2, CO2, and CO from steel making Blast Furnace-Basic Oxygen Furnace and Coke Oven off-gases, major contributors to anthropogenic carbon emissions, are presented. Three PSA units are modeled on Aspen Adsorption V14, each utilising dedicated adsorbents and configurations tailored for the target gas. Model validation is successfully conducted by comparing breakthrough simulation results with experimental data. The simulation results demonstrate that the PSA systems effectively separate H2

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Stover, Ed, Dominick Scotto, Chris Wilson, and Masoud Salyani. "Pesticide Spraying in Indian River Grapefruit: II. Overview of Factors Influencing Spray Efficacy and Off-target Deposition." HortTechnology 13, no. 1 (2003): 166–77. http://dx.doi.org/10.21273/horttech.13.1.0166.

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Foliar application of spray materials is an integral component of commercial citrus production. An intensive assessment of spray application practices has been stimulated by low fruit value and increased concern about potential surface water contamination in the Indian River citrus region of Florida. Many publications report research results regarding distribution of spray materials within orchards and off-target deposition, but interpretation is challenging because so many factors influence spray results, and integrating this information into practical recommendations is difficult. Canopy geo

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Ramaprasad, Jaya, Ming-Yi Tsai, Kai Elgethun, et al. "The Washington aerial spray drift study: assessment of off-target organophosphorus insecticide atmospheric movement by plant surface volatilization." Atmospheric Environment 38, no. 33 (2004): 5703–13. http://dx.doi.org/10.1016/j.atmosenv.2004.04.035.

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Tan, E‐Pien, Yilong Li, Martin Del Castillo Velasco‐Herrera, Kosuke Yusa, and Allan Bradley. "Off‐target assessment of CRISPR ‐ C as9 guiding RNA s in human i PS and mouse ES cells." genesis 53, no. 2 (2014): 225–36. http://dx.doi.org/10.1002/dvg.22835.

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Lema, Martin. "Regulatory Assessment of Off-Target Changes and Spurious DNA Insertions in Gene-Edited Organisms for Agri-Food Use." Journal of Regulatory Science 9, no. 1 (2021): 1–15. http://dx.doi.org/10.21423/jrs-v09i1lema.

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Worldwide, an increasing number of regulatory systems have begun to consider applications for the authorization of activities involving gene-edited organisms for agri-food use. Although a handful of countries have made advances in establishing regulatory criteria and gathering practical experience in this regard, there is still a general need for regulatory cooperation concerning capacity building and development of harmonized criteria. Consequently, many biotechnology regulators need to quickly become more acquainted with the numerous technological possibilities enclosed under the concept of

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Masinza, Faith Lugulu, Paul Maku Gichohi, and Catherine Mueni Nzioka. "Assessment of Digital Literacy Skills for Supporting Off-Campus Teaching at Selected University Libraries in Kenya." International Journal of Professional Practice 10, no. 2 (2022): 81–91. https://doi.org/10.71274/ijpp.v10i2.228.

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University libraries in Kenya are deficiently prepared to launch and operationalize full-fledged off-campus information products and services; hence, the wavering support for off-campus learning and teaching. The purpose of this study was to assess the digital literacy skills of selected university libraries in supporting off-campus teaching. The study was anchored on the technological pedagogical content knowledge model. Data was obtained from Kenyatta, Nairobi and Kenya College of Accountancy universities. A descriptive survey research design was employed and the target population was 127 un

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Slapak, Etienne J., Mouad el Mandili, Marieke S. Ten Brink, Alexander Kros, Maarten F. Bijlsma, and C. Arnold Spek. "Preclinical Assessment of ADAM9-Responsive Mesoporous Silica Nanoparticles for the Treatment of Pancreatic Cancer." International Journal of Molecular Sciences 24, no. 13 (2023): 10704. http://dx.doi.org/10.3390/ijms241310704.

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Pancreatic adenocarcinoma (PDAC) remains largely refractory to chemotherapeutic treatment regimens and, consequently, has the worst survival rate of all cancers. The low efficacy of current treatments results largely from toxicity-dependent dose limitations and premature cessation of therapy. Recently, targeted delivery approaches that may reduce off-target toxicities have been developed. In this paper, we present a preclinical evaluation of a PDAC-specific drug delivery system based on mesoporous silica nanoparticles (MSNs) functionalized with a protease linker that is specifically cleaved by

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