Academic literature on the topic 'Oleanic acid'

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Journal articles on the topic "Oleanic acid"

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Carvalho, Luis, and J. Seita. "A New Oleanic Acid Derivative fromSecurinega tinctoria." Planta Medica 59, no. 04 (August 1993): 369–72. http://dx.doi.org/10.1055/s-2006-959704.

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Nie, Xu-Liang, Zhong-Ping Yin, and Xin-Chen Shang-Guan. "Crystal structure of benzyl-2-oxo-oleanic acid, C37H52O4." Zeitschrift für Kristallographie - New Crystal Structures 229, no. 4 (December 1, 2014): 473–75. http://dx.doi.org/10.1515/ncrs-2014-0162.

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Su, Dan, Yu-qiao Gao, Wei-bo Dai, Ying Hu, Yan-fen Wu, and Quan-xi Mei. "Helicteric Acid, Oleanic Acid, and Betulinic Acid, Three Triterpenes fromHelicteres angustifoliaL., Inhibit Proliferation and Induce Apoptosis in HT-29 Colorectal Cancer Cells via Suppressing NF-κB and STAT3 Signaling." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/5180707.

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Colorectal cancer (CRC) is one of the most common malignancies and most frequent cause of cancer death worldwide. The activation of both NF-κB and STAT3 signaling and the crosstalk between them play an important role in colorectal tumor.Helicteres angustifoliaL. is a type of commonly used Chinese medicinal herb and possesses a wide variety of biological activities. In the present study, we investigate the effects of three triterpenes fromH. angustifolia(HT) such as helicteric acid (HA), oleanic acid (OA), and betulinic acid (BA), on inhibiting CRC progression. Our results showed that HT extracts could decrease proliferation and induce apoptosis in HT-29 colorectal cancer cells. Moreover, HT extracts could suppress LPS-triggered phosphorylation of IKK, IκB, and NF-κB, attenuate IL-6-induced phosphorylation of JAK2 and STAT3, and suppress the expression of c-Myc, cyclin-D1, and BCL-xL, the downstream gene targets of NF-κB and STAT3. Therefore, HT extracts showed potent therapeutic and antitumor effects on CRC via inhibiting NF-κB and STAT3 signaling.
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Sogno, I., N. Vannini, G. Lorusso, R. Cammarota, D. M. Noonan, and A. Albini. "P12 Anti-angiogenic activity of a novel class of chemopreventive compounds, oleanic acid terpenoids." European Journal of Cancer Supplements 6, no. 3 (March 2008): 45. http://dx.doi.org/10.1016/s1359-6349(08)70244-1.

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Alvarado, Helen L., Ana C. Calpena, María L. Garduño-Ramírez, Raúl Ortiz, Consolación Melguizo, José C. Prados, and Beatriz Clares. "Nanoemulsion Strategy for Ursolic and Oleanic Acids Isolates from Plumeria Obtusa Improves Antioxidant and Cytotoxic Activity in Melanoma Cells." Anti-Cancer Agents in Medicinal Chemistry 18, no. 6 (November 12, 2018): 847–53. http://dx.doi.org/10.2174/1871520618666180111151846.

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Background: Triterpenoids are an important class of natural bioactive products present in many medicinal plants. Objective: The aim of present study is to investigate the antioxidant and anticarcinogenic potential of Oleanolic Acid (OA) and Ursolic Acid (UA) on B16 murine melanoma cell line isolated from Plumeria obtusa, free and loaded in a nanoemulsion (NEm) system. Methods: The nanoemulsion was characterized by dynamic light scattering, transmission electron microscopy. The viscosity was also evaluated. The antioxidant activity was determined by the reduction of 2,2-diphenyl-2- picrylhydrazyl (DPPH) free radical. In vitro proliferation studies were determined using the sulforhodamine-B method. Results: OA/UA natural mixture exhibited high percentage of inhibition of DPPH (86.06% and 85.12%, with and without irradiation). Percentages of inhibition higher than 85% in samples with and without ultraviolet irradiation were recorded when loaded in the NEm system. The natural mixture incorporated into the NEm showed cytotoxic activity from 2.9 µM, whereas the free compounds from 17.4 µM. Conclusion: We conclude that these pentacyclic triterpenes loaded in a NEm system could be considered as a new potential tool for further investigation as anticancer agents.
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Castellano, Leonardo, Rosabel S. de Correa, Eduardo Martínez, and Jose´ S. Calderon. "Oleanane Triterpenoids from Cedrela montana (Meliaceae)." Zeitschrift für Naturforschung C 57, no. 7-8 (August 1, 2002): 575–78. http://dx.doi.org/10.1515/znc-2002-7-804.

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Two new oleanane-type triterpenes, characterized as 3-oxo-11α,12α-epoxy-oleanan-28,13β- olide and 3-oxo-olean-11-en-28,13β-olide , were isolated from the fruits and seeds of Cedrela montana (Meliaceae). In addition, the known compounds oleanonic acid , a mixture of β-sitosterol and stigmasterol, and the limonoid photogedunin were also isolated. The structures of the new compounds were established by spectroscopic methods, including 2D NMR.
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Li, Sumei, Xiuhua Jia, Xintian Shen, Zhuwen Wei, Zhiyan Jiang, Yixian Liao, Yiming Guo, Xiaojun Zheng, Guohua Zhong, and Gaopeng Song. "Structure-activity relationships of 3-O-β-chacotriosyl oleanic acid derivatives as entry inhibitors for highly pathogenic H5N1 influenza virus." Bioorganic & Medicinal Chemistry 25, no. 16 (August 2017): 4384–96. http://dx.doi.org/10.1016/j.bmc.2017.06.025.

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Cunha, Luis C. Scalon, Márcio L. Andrade e. Silva, Niege A. J. Cardoso Furtado, Adriana H. C. Vinhólis, Carlos H. Gomes Martins, A. da Silva Filho, and Wilson R. Cunha. "Antibacterial Activity of Triterpene Acids and Semi-Synthetic Derivatives against Oral Pathogens." Zeitschrift für Naturforschung C 62, no. 9-10 (October 1, 2007): 668–72. http://dx.doi.org/10.1515/znc-2007-9-1007.

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Triterpene acids (ursolic, oleanoic, gypsogenic, and sumaresinolic acids) isolated from Miconia species, along with a mixture of ursolic and oleanolic acids and a mixture of maslinic and 2-α-hydroxyursolic acids, as well as ursolic acid derivatives were evaluated against the following microorganisms: Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis, which are potentially responsible for the formation of dental caries in humans. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) during the evaluation of the antibacterial activity. All the isolated compounds, mixtures, and semi-synthetic derivatives displayed activity against all the tested bacteria, showing that they are promising antiplaque and anticaries agents. Ursolic and oleanolic acids displayed the most intense antibacterial effect, with MIC values ranging from 30 μg/mL to 80 μg/mL. The MIC values of ursolic acid derivatives, as well as those obtained for the mixture of ursolic and oleanolic acids showed that these compounds do not have higher antibacterial activity when compared with the activity observed with either ursolic acid or oleanolic acid alone. With regard to the structure-activity relationship of triterpene acids and derivatives, it is suggested that both hydroxy and carboxy groups present in the triterpenes are important for their antibacterial activity against oral pathogens.
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Joshi, Prashant, Omprakash Tanwar, Sujit Rambhade, Mukesh Bhaisare, and Deepti Jain. "2-D QSAR studies of steroidal natural products oleanic acid and their semisynthetic derivatives as potent protein tyrosine phosphatase 1B inhibitors." Medicinal Chemistry Research 21, no. 3 (January 7, 2011): 351–61. http://dx.doi.org/10.1007/s00044-010-9529-5.

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Hu, Yufang, Zhaohui Zhang, Jiaxing Li, Huabin Zhang, Lijuan Luo, and Shouzhuo Yao. "Electrochemical imprinted sensor for determination of oleanic acid based on poly (sodium 4-styrenesulfonate-co-acrylic acid)-grafted multi-walled carbon nanotubes-chitosan and cobalt hexacyanoferrate nanoparticles." Biosensors and Bioelectronics 31, no. 1 (January 2012): 190–96. http://dx.doi.org/10.1016/j.bios.2011.10.016.

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Dissertations / Theses on the topic "Oleanic acid"

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Wicht, Merril Margaret. "Oleanic acid: its isolation and derivatisation to potential antimicrobial compounds." Thesis, Cape Peninsula University of Technology, 2007. http://hdl.handle.net/20.500.11838/732.

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A thesis submitted to the Cape Peninsula University of Technology in fulfilment of the requirements for the MASTERS DEGREE IN TECHNOLOGY (CHEMISTRY) Department of Chemistry January 2007
An increasing number of natural products possessing the oleanolic acid moiety have been shown to demonstrate a wide spectrum of biological activity. This thesis deals with the extraction and isolation of oleanolic acid from Syzigium aromaticum and the examination of its stereochemistry and crystal structure by X-ray diffraction. The synthetic routes used for converting functional groups on the oleanolic acid molecule to afford derivatives are described in Chapter 5. Oleanolic acid and its derivatives were evaluated for antimicrobial activity. Three different procedures viz. Kirby-Bauer, Broth dilution and Tetrazolium salt chemosensitivity were used. Acceptable results were obtained from the last method and these were used to arrive at conclusions regarding this study.
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Cheng, Keguang. "Conception, synthèse et évolution biologique des dérivés d'acide oléanolique." Cachan, Ecole normale supérieure, 2010. http://www.theses.fr/2010DENS0025.

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La diabète de type 2 est associée avec des désordres du métabolisme de glucose dans le foie et les système périphériques, résultant un niveau trop élevé du glucose dans le sang qui est responsable des conséquences graves à long terme. Un agent anti-diabétique idéal doit être capable de réduire le taux de glucose en états- alimentés et à jeun. Le contrôle du métabolisme hépatique du glucose est l'une des principales voies utilisées par l'insuline pour maintenir l'homéostasie du glucose dans le sang. Parmi les différentes possibilités d'influencer la production hépatique du glucose, l'inhibition de la glycogène phosphorylase (GP), enzyme responsable de la dégradation du glycogène, est une approche prometteuse pour le traitement de diabète de type 2. Plusieurs inhibiteurs de se sont montrent efficaces à baisser le taux de glucose dans des animaux et en essai clinique. Récemment, nous avons montré que l'acide oléanolique et d'autres triterpènes pentacycliques représentent une nouvelle classe d'inhibiteurs de glycogène phosphorylase. La structure aux rayons X révèlent que les triterpènes pentacycliques comme l'acide asiatique et l'acide maslinique fixent au site allostérique de OP. Les efforts intensifs ont été réalisés sur la modification structurale des triterpènes naturels afin de trouver des agents préventifs et thérapeutiques. Nous espérions que les dérives de triterpènes pentacycliques puissent être utilisés comme des nouveaux médicaments pour la régulation du métabolisme de glucose. Dans cette étude, nous avons synthétisé plusieurs dérivés de l'acide oléanolique et des conjugués avec des sucres et des nucléosides. Leur propriété inhibitrice vis-à-vis de la glycogène phosphorylase a été évalué avec la GPa du muscle du lapin (RMGPa). Certaines molécules présentent une inhibition de l'ordre de micromolaire
Type 2 diabetes mellitus is associated with disorelers in glucose metabolism by the liver and periphery resulting in elevated blood glucose levels which, in turn, are responsible for fatal long term complications. An ideal anti-diabetic agent should be capable of lowering blood glucose in both fed and fasted states. Control of die hepatic glycogen metabolism is one of the key events through which insulin maintains blood glucose homeostasis. Among other means for influencing glucose production in the liver, inhibition of glycogen phosphorylase (GP), the rate limiting enzyme of glycogen degradation, has been regarded as a promising therapeutic approach ta the treatment of type 2 diabetes. A couple of GP inhibitors have shown efficacy in lowering blood glucose in animal models and clinical trials. We have recently reported that oleanolic acid and related pentacyclic triterpenes represented a new class of inhibitors of glycogen phosphorylases. X-Ray crystallographic studies revealed the molecular basis of their inhibitory effect demonstrating that pentacyclic triterpenes such as asiatic and maslinic acids bind ta OP at the allosteric site. On the other hand, structural modifications based on natural triterpenes have been extensively explored ta find more patent pentacyclic triterpenes as preventive and therapeutic agents. Therefore, we believe that pentacyclic triterpenes will be used as new drugs in regulating glucose and lipid metabolism one day. Thus, in this study, we have synthesized several oleanolic acid derivatives and conjugates with sugar and nucleosides, and evaluated GP inhibitory activity against RMGPa. Some molecules exhibited inhibition in micromolar range. The structural analyses of these cornpounds can be further exploited (by chemical modification) towards the development of better GP inhibitors
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Abukhattala, Emhemed Mohamed. "Ursolic acid and oleanolic acid as novel therapeutic agents in breast cancer." University of the Western Cape, 2015. http://hdl.handle.net/11394/5053.

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Philosophiae Doctor - PhD
Breast cancer is one of the most common cancers among women in South Africa and the second leading cause of cancer death after lung cancer. According to the American Cancer Society 2015, women have a 12% chance of developing invasive breast cancer and a 3% chance of dying from it. Despite the wide variety of breast cancers e.g. lobular carcinoma in situ (LCIS) and ductal carcinoma in situ (DCIS), many share the same etiology and target tissue. Estrogen related carcinogenesis with regard to breast cancer typically results from the activation of distinct signalling pathways. These pathways are not mutually exclusive and are often constituted by receptor mediated stimulation of cell proliferation caused by specific transcriptional gene activation, reactive oxygen species (ROS) formation causing DNA damage and consequently mutations. The molecular pathways that cause drug resistance are not fully understood and the search continues to find novel targets for treatment. The effects of non-toxic triterpenes, oleanolic acid and ursolic acid and the role of autophagy and apoptosis as mechanisms to overcome drug resistance in breast cancer were studied in vitro in MCF-7 breast cancer cells and MCF10A breast cells. In this study the first aim was to establish the influence of OA and UA on cell growth and to see if opposing proliferation patterns could observed between the presumably ERɑ negative (ERɑ/ß -/+) MCF-10A and ERɑ positive (ERɑ/ß +/+) MCF-7 cells. This was followed by morphology studies to establish the possible presence of cytotoxicity and examination of molecular pathways contributing to the anti-cancerous properties of UA and OA and their validity as therapeutic agents. The MCF-7 breast cancer cell line and the immortalized normal mammary cell line, MCF-10A were treated with different concentrations of UA and OA for 6hrs, 12hrs, 24hrs, 48hrs, and 72hrs respectively. Cell morphology was studied in hematoxylin and eosin as well as Hoechst and acridine orange stained cells and viability was measured using crystal violet staining. Molecular techniques employed included the Tali® Apoptosis - and the cellROX assays, flow cytometry and western blotting. Morphological, viability and apoptotic studies have shown that at their lowest concentration, both UA and OA have anti-proliferative and apoptotic effects on MCF-7 and to a lesser extent on MCF-10A. Flow cytometric analysis of treated cells has demonstrated cell arrest in the S- and G2/M phase. The MCF-7 and MCF-10A cells growth inhibition effect may be due to increased autophagy and apoptosis as an alternative to decreased proliferation in MCF-7 cells. This possibility should be evaluated in further studies. The results showed that UA was more effective OA in decreasing cell numbers and it may be applied as treatment for breast cancer. Our observation has shown the treatment with OA and UA increased cell death in MCF-7 cells.The opposing proliferation patterns observed between the presumably ERɑ negative (ERɑ/ß -/+) MCF-10A and ERɑ positive (ERɑ/ß +/+) MCF-7 cells could possibly be ascribed to ERß forming homodimers that may facilitate proliferation, whereas ERɑ/ß heterodimers (expressed in 59% of breast cancers) are frequently associated with the ERɑ antagonising actions of ERß. The results indicate a trend towards biphasic and anti- proliferative effects of the reactants in breast cancer cells which may contribute towards the development of anti- cancer therapies. However, further work is must be done to identify the OA and UA mechanism(s) responsible for anticancer activity.
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Silva, Mariana Rosa da. "Padronização de método colorimétrico para avaliação de atividade biológica de substâncias sobre formas taquizoítas de Toxoplasma gondii, com a avaliação de triterpenos ácidos sobre o parasito." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29052009-154530/.

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Toxoplasma gondii é um protozoário pertencente ao filo Apicomplexa, de distribuição mundial, e que infecta diversas espécies hospedeiros, como mamíferos e aves, possuindo como hospedeiros definitivos o gato e outros felídeos, enquanto o homem e outros animais são os seus hospedeiros intermediários. O tratamento é feito, na maioria das vezes, com uma combinação de sulfadiazina e pirimetamina, agindo na via metabólica do ácido fólico, o qual é necessário para a biossíntese de purinas, pirimidinas e certos aminoácidos. Propusemos estabelecer um protocolo de avaliação sobre esse protozoário, assim como padronizarmos uma metodologia por espectroscopia para uma rápida avaliação ou triagem de substâncias potencialmente ativas contra o parasito. Verificamos a atividade das substâncias ácido ursólico e ácido oleanóico, as quais já demonstraram atividade biológica sobre outras espécies de protozoários, como Plasmodium, Trypanosoma cruzi e Leishmania sp. em sistemas in vitro e in vivo. A metodologia colorimétrica pelo MTT, pelo Alamar Blue®, do kit CyQUANT® NF e a contagem manual em meio líquido das formas taquizoítas do parasito em ensaios biológicos in vitro mostram-se inviáveis, pois há grande dificuldade em manter a integridade do parasita em meio de cultura líquido, o qual se mostra sensível à adição de qualquer outro componente que não aqueles necessários para manter sua viabilidade em ambiente extracelular. O ácido ursólico mostrou-se potencialmente ativo in vitro sobre formas intracelulares de T. gondii. Entretanto, o contato de células infectadas com as substâncias avaliadas por um período de 48 horas não resultou em diminuição mais acentuada na porcentagem de células infectadas do que a ocorrida no tratamento de 24 horas. A comparação dos resultados do tratamento pós infecção celular por 24 horas e do pré-tratamento das formas taquizoítas houve diferenças significativas, indicando principalmente maior ação do pré-tratamento sobre T. gondii. Quando administrado na dose de 7 mg/kg/ dia a camundongos infectados, o ácido ursólico não apresentou atividade sobre o parasita.
Toxoplasma gondii is an Apicomplexa protozoan, of worldwide distribution, that infects several species, from mammalians to birds; its definitive hosts are the cats and other felines, while man and other animals are considered as intermediate hosts. Treatment is, generally, a combination of sulfadiazine and pyrimethamine, triggering the metabolic pathway of folic acid, which is necessary for certain purines, pirimidines and aminoacids biosynthesis. We have proposed an evaluation protocol on this protozoan, and also to establish a methodology by spectroscopy for rapid evaluation of pottentialy bioactive substances against the parasite. The ursolic acid and oleanoic acid bioactivity were tested. These substances have already demonstrated to be effective on another protozoan species, like Plasmodium, Trypanosoma cruzi e Leishmania sp., either in vitro or in vivo. The colorimetric methodology by MTT, Alamar Blue®, CyQUANT® NF kit and manual counting of tachyzoite forms in liquid culture medium showed to be unviable, because there is a great difficult to maintain the parasite viability in liquid culture medium, wich one is sensible to addition of any other component different of that necessary for its survival in extracelular ambient. Ursolic acid was pottentialy active on T. gondii intracellular forms. However, the infected cells in contact with the tested substances for a period of 48 hours did not show a statistical greater reduction as compared to infected cells which underwent treatment for 24 hours. Significant results were observed when comparing pre-treatment of tachyzoite forms and treatment for 24 hours post-cellular infection. Our data pointed in the direction that pre-treatment exerted a higher effectiveness. Any parasiticidal activity was observed when ursolic acid on a concentration of 7 mg/kg/day was administered to infected mice.
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Man, Kwun-wai Dede, and 文冠慧. "Oleanolic acid delivery using biodegradable nanoparticles for cancer therapy." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2015. http://hdl.handle.net/10722/208550.

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Sousa, Paloma LeÃo de. "Trypanocidal effect of betulinic and oleanolic acids." Universidade Federal do CearÃ, 2017. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19425.

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CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior
A doenÃa de Chagas à uma doenÃa negligenciada causada pelo parasito Trypanosoma cruzi e constitui um problema de saÃde pÃblica em todo o mundo. O tratamento atual à restrito pelos efeitos colaterias frequentes e pela eficÃcia limitada do benzonidazol. O Ãcido betulÃnico (AB) e Ãcido oleanÃlico (AO), sÃo triterpenos, existentes em diversas plantas medicinais e exibem uma grande variedade de atividades biolÃgicas e farmacolÃgicas, incluindo efeito em tripanossomatÃdeos. O objetivo do presente estudo foi avaliar o efeito tripanocida e mecanismo de aÃÃo dos Ãcidos betulÃnico e oleanÃlico em cepa Y de Trypanosoma cruzi in vitro. O efeito tripanocida do AB e AO (1,56 - 200 μM) foram avaliadas durante 24, 48 e 72 horas, sob formas epimastigotas, tripomastigotas e amastigotas de T. cruzi. A viabilidade da cÃlula LLCMK2, tratada com AB e AO (200 - 1600 μM), foi avaliada durante 24 h atravÃs do teste MTT. Os ensaios de mecanismo de aÃÃo foram realizados nas formas epimastigotas tratadas com CI50 do AB e AO durante 24 h, e marcadas com anexina V/7AAD, Rho123, H2DCFDA, Laranja de Acridina e MDC de acordo com as instruÃÃes do fabricante e analisadas por citometria de fluxo e microscopia confocal. Os dados foram analisados utilizando ANOVA com pÃs-teste de Bonferroni ou teste t Student, * p≤0,05. O AB inibiu o crescimento de formas epimastigotas em 24h (CI50 = 73,43 μM; BZ = 218 μM), 48h (CI50 = 119,8 μM; BZ = 61μM) e 72h (CI50 = 212,2 μM; BZ = 16,5 μM) de incubaÃÃo; inibiu a viabilidade de tripomastigotas (CI50 = 51,88 μM; BZ = 257μM) em 24h. O AO inibiu o crescimento de epimastigotas em 24h (CI50 = 11,66 μM; BZ = 218 μM), 48h (CI50 = 43,15 μM; BZ = 61 μM) e 72h (CI50 = 43,05 μM; BZ = 16,5μM) de incubaÃÃo; inibiu a viabilidade de tripomastigotas (IC50 = 13,97 μM; BZ = 257 μM) em 24h. O AB e AO diminuiram o percentual de cÃlulas infectadas e reduziram o nÃmero de amastigotas por cÃlulas nos tratamentos com 24h e 48 h, respectivamente. Ambos terpenos nÃo apresentaram toxicidade sobre as cÃlulas LLCMK2 nas concentraÃÃes utilizadas. A anÃlise do mecanismo de morte celular do parasito tratado com AB mostrou alteraÃÃes no potencial da membrana mitocondrial, alteraÃÃes na integridade da membrana celular, aumento da formaÃÃo de espÃcies reativas de oxigÃnio e detecÃÃo de compartimentos acÃdicos. O tratamento com AO demonstrou detecÃÃo de compartimentos acÃdicos e de vacuÃlos autofÃgicos. Nossos resultados demonstram que o AB e AO apresentam efeito tripanocida sobre todas as formas evolutivas de cepa Y de T. cruzi, sugerindo que o mecanismo de morte celular do AB à via necrose e do AO via autofagica nas formas epimastigotas.
A doenÃa de Chagas à uma doenÃa negligenciada causada pelo parasito Trypanosoma cruzi e constitui um problema de saÃde pÃblica em todo o mundo. O tratamento atual à restrito pelos efeitos colaterias frequentes e pela eficÃcia limitada do benzonidazol. O Ãcido betulÃnico (AB) e Ãcido oleanÃlico (AO), sÃo triterpenos, existentes em diversas plantas medicinais e exibem uma grande variedade de atividades biolÃgicas e farmacolÃgicas, incluindo efeito em tripanossomatÃdeos. O objetivo do presente estudo foi avaliar o efeito tripanocida e mecanismo de aÃÃo dos Ãcidos betulÃnico e oleanÃlico em cepa Y de Trypanosoma cruzi in vitro. O efeito tripanocida do AB e AO (1,56 - 200 μM) foram avaliadas durante 24, 48 e 72 horas, sob formas epimastigotas, tripomastigotas e amastigotas de T. cruzi. A viabilidade da cÃlula LLCMK2, tratada com AB e AO (200 - 1600 μM), foi avaliada durante 24 h atravÃs do teste MTT. Os ensaios de mecanismo de aÃÃo foram realizados nas formas epimastigotas tratadas com CI50 do AB e AO durante 24 h, e marcadas com anexina V/7AAD, Rho123, H2DCFDA, Laranja de Acridina e MDC de acordo com as instruÃÃes do fabricante e analisadas por citometria de fluxo e microscopia confocal. Os dados foram analisados utilizando ANOVA com pÃs-teste de Bonferroni ou teste t Student, * p≤0,05. O AB inibiu o crescimento de formas epimastigotas em 24h (CI50 = 73,43 μM; BZ = 218 μM), 48h (CI50 = 119,8 μM; BZ = 61μM) e 72h (CI50 = 212,2 μM; BZ = 16,5 μM) de incubaÃÃo; inibiu a viabilidade de tripomastigotas (CI50 = 51,88 μM; BZ = 257μM) em 24h. O AO inibiu o crescimento de epimastigotas em 24h (CI50 = 11,66 μM; BZ = 218 μM), 48h (CI50 = 43,15 μM; BZ = 61 μM) e 72h (CI50 = 43,05 μM; BZ = 16,5μM) de incubaÃÃo; inibiu a viabilidade de tripomastigotas (IC50 = 13,97 μM; BZ = 257 μM) em 24h. O AB e AO diminuiram o percentual de cÃlulas infectadas e reduziram o nÃmero de amastigotas por cÃlulas nos tratamentos com 24h e 48 h, respectivamente. Ambos terpenos nÃo apresentaram toxicidade sobre as cÃlulas LLCMK2 nas concentraÃÃes utilizadas. A anÃlise do mecanismo de morte celular do parasito tratado com AB mostrou alteraÃÃes no potencial da membrana mitocondrial, alteraÃÃes na integridade da membrana celular, aumento da formaÃÃo de espÃcies reativas de oxigÃnio e detecÃÃo de compartimentos acÃdicos. O tratamento com AO demonstrou detecÃÃo de compartimentos acÃdicos e de vacuÃlos autofÃgicos. Nossos resultados demonstram que o AB e AO apresentam efeito tripanocida sobre todas as formas evolutivas de cepa Y de T. cruzi, sugerindo que o mecanismo de morte celular do AB à via necrose e do AO via autofagica nas formas epimastigotas.
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Wicht, Merrill Margaret. "Oleanolic acid: its isolation and derivatisation to potential antimicrobial compounds." Thesis, Cape Peninsula University of Technology, 2007. http://hdl.handle.net/20.500.11838/738.

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Thesis (MTech (Chemistry))--Cape Peninsula University of Technology, 2007
An increasing number of natural products possessing the oleanolic acid moiety have been shown to demonstrate a wide spectrum of biological activity. This thesis deals with the extraction and isolation of oleanolic acid from Syzigium aromaticum and the examination of its stereochemistry and crystal structure by X-ray diffraction. The synthetic routes used for converting functional groups on the oleanolic acid molecule to afford derivatives are described in Chapter 5. Oleanolic acid and its derivatives were evaluated for antimicrobial activity. Three different procedures viz. Kirby-Bauer, Broth dilution and Tetrazolium salt chemosensitivity were used. Acceptable results were obtained from the last method and these were used to arrive at conclusions regarding this study.
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Haas, Christiane, Karl-Christoph Hengelhaupt, Sibylle Kümmritz, Thomas Bley, Atanas Pavlov, and Juliane Steingroewer. "Salvia suspension cultures as production systems for oleanolic and ursolic acid." Springer, 2014. https://tud.qucosa.de/id/qucosa%3A30075.

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Oleanolic and ursolic acid (OA and UA) are triterpenic acids with diverse biological activities that are of interest to the pharmaceutical industry. To investigate the scope for producing these compound using cell suspension cultures of Salvia species, calli from S. officinalis, S. virgata and S. fruticosa were induced using several plant growth regulator (PGR) combinations. Eleven lines were selected for suspension induction from a pool of calli. Six suspension cultures were established successfully and cultivated in the Respiration Activity MOnitoring System® (RAMOS®) to obtain online data on their growth kinetics and to establish appropriate sampling schedules for the determination of their OA and UA production. Based on their observed growth behaviour, OA and UA contents, and aggregation properties, one suspension culture from each studied Salvia species was selected for further optimisation. The μmax values for these suspension cultures ranged from 0.20 to 0.37°d-1, their OA and UA contents were greater than 1.3 and 1.2 mg g-1, respectively, and they afforded maximum volumetric yields of 21.0 mg l-1 for OA and 32.8 mg l-1 for UA. These results will be useful in the development of a refined Salvia suspension-based process for OA and UA production.
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Haas, Christiane, Karl-Christoph Hengelhaupt, Sibylle Kümmritz, Thomas Bley, Atanas Pavlov, and Juliane Steingroewer. "Salvia suspension cultures as production systems for oleanolic and ursolic acid." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-216350.

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Oleanolic and ursolic acid (OA and UA) are triterpenic acids with diverse biological activities that are of interest to the pharmaceutical industry. To investigate the scope for producing these compound using cell suspension cultures of Salvia species, calli from S. officinalis, S. virgata and S. fruticosa were induced using several plant growth regulator (PGR) combinations. Eleven lines were selected for suspension induction from a pool of calli. Six suspension cultures were established successfully and cultivated in the Respiration Activity MOnitoring System® (RAMOS®) to obtain online data on their growth kinetics and to establish appropriate sampling schedules for the determination of their OA and UA production. Based on their observed growth behaviour, OA and UA contents, and aggregation properties, one suspension culture from each studied Salvia species was selected for further optimisation. The μmax values for these suspension cultures ranged from 0.20 to 0.37°d-1, their OA and UA contents were greater than 1.3 and 1.2 mg g-1, respectively, and they afforded maximum volumetric yields of 21.0 mg l-1 for OA and 32.8 mg l-1 for UA. These results will be useful in the development of a refined Salvia suspension-based process for OA and UA production.
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Prasanna, Kumar Divya. "Regulation of Pancreatic α and β Cell Function by the Bile Acid Receptor TGR5." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3591.

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The discovery that bile acids act as endogenous ligands of the membrane receptor TGR5 and the nuclear receptor FXR increased their significance as regulators of cholesterol, glucose and energy metabolism. Activation of TGR5, expressed on enteroendocrine L cells, by bile acids caused secretion of GLP-1, which stimulates insulin secretion from pancreatic β cells. Expression of TGR5 on pancreatic islet cells and the direct effect of bile acids on the endocrine functions of pancreas, however, are not fully understood. The aim of this study was to identify expression of TGR5 in pancreatic islet cells and determine the effect of bile acids on insulin secretion. Expression of TGR5 was identified by quantitative PCR and western blot in islets from human and mouse, and in α (αTC1-6) and β (MIN6) cells. Release of insulin, glucagon and GLP-1 were measured by ELISA. The signaling pathways coupled to TGR5 activation were identified by direct measurements such as stimulation of G proteins, adenylyl cyclase activity, PI hydrolysis and intracellular Ca2+ in response to bile acids; and confirmed by the use of selective inhibitors that block specific steps in the signaling pathway. Our studies identified expression of TGR5 receptors in β cells and demonstrated that activation of these receptors by both pharmacological ligands (oleanolic acid (OA) and INT-777) and physiological ligand (lithocholic acid, LCA) induced insulin secretion. TGR5 receptors are also expressed in α cells and, activation of TGR5 by OA, INT-777 and LCA at 5 mM glucose induced release of glucagon, which is processed from proglucagon by the selective expression of prohormone convertase 2 (PC2). However, under hyperglycemia, activation of TGR5 in α cells augmented the glucose-induced increase in GLP-1 secretion, which in turn, stimulated insulin secretion. Secretion of GLP-1 from α cells reflected TGR5-mediated increase in PC1 promoter activity and PC1 expression, which selectively converts proglucagon to GLP-1. The signaling pathway activated by TGR5 to mediate insulin and GLP-1 secretion involved Gs/cAMP/Epac/PLC-ε/Ca2+. These results provide insights into the mechanisms involved in the regulation of pancreatic α and β cell function by bile acids and may lead to new therapeutic avenues for the treatment of diabetes.
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Book chapters on the topic "Oleanic acid"

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Zhou, Qi-Meng, and Guan-Hua Du. "Oleanolic Acid." In Natural Small Molecule Drugs from Plants, 433–37. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8022-7_72.

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"Oleanolic Acid." In Encyclopedia of Cancer, 2600. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-16483-5_4198.

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Waheed, Ammara, Sumia Akram, Muhammad Mushtaq, and Ahmad Adnan. "Oleanolic acid and ursolic acid." In A Centum of Valuable Plant Bioactives, 93–115. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822923-1.00021-2.

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Akinyinka Akinwumi, Kazeeem, Oluwole Olusoji Eleyowo, and Omolara Omowunmi Oladipo. "A Review on the Ethnobotanical Uses, Phytochemistry and Pharmacology Effect of Luffa cylindrica." In Pharmacognosy - Medicinal Plants [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98405.

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Luffa cylindrica, popularly known as sponge gourd is a tropic and sub-tropical fibrous plant with fruits containing black seeds. The fruit is consumed by humans as a vegetable in many parts of Asia, while different parts of the plant are used for cosmetics and as medicine in many parts of the globe. The plant has been used in the treatment of many ailments including nose cancer, snake venom, wound healing, edema, enterobiasis, filaria, whooping cough, stomach upset, stomach pain and malaria. Many health-promoting compounds such as flavonoids (apigenin-7- glucuronide luteolin-7-O-β-D-glucuronide methyl ester, -O-feruloyl-β-D-glucose, luteolin-7-O-β-D-glucuronide methyl ester), phenolics acids (p-Coumaric, gallic, caffeic, chlorogenic), triterpenoids (oleanolic acid and echinocystic acid), saponins (Lucyoside A-M), tannins (catechin), ribosome-inactivating proteins (α- luffin), carotenoids (9 -cis neoxanthin, all-trans-lutein, all-trans-β-carotene), chlorophylls (chlorophyll a and b, pheophytin), cucurbitacin B and gypsogenin have been detected or isolated from different parts of the plants. Extracts of the plant and isolated compounds have wide spectrum pharmacological activities and have been shown to possess antiemetic, antidiabetic, antiviral, wound healing, anticancer, antipyretic, anti-inflammatory, antifungal, anti-bacteria, anthelmintic, hypoglycemic and antihyperglycemic, anti-inflammatory, antioxidant activity, and hepato-protective effects in animal models. However, further information is needed on its safety and mechanisms of action. The present article is an updated review of the ethnobotanical uses, pharmacological actions, phytochemistry, safety, and future application of Luffa cylindrica in translational medicine.
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Liu, Jun, Rajkumar Rajendram, and Luyong Zhang. "Effects of Oleanolic Acid and Maslinic Acid on Glucose and Lipid Metabolism." In Olives and Olive Oil in Health and Disease Prevention, 1423–29. Elsevier, 2010. http://dx.doi.org/10.1016/b978-0-12-374420-3.00158-3.

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Rodriguez-Rodriguez, Rosalia, and Valentina Ruiz-Gutiérrez. "Vasorelaxant Effects of Oleanolic Acid and Erythrodiol in Pomace Olive Oil." In Olives and Olive Oil in Health and Disease Prevention, 813–20. Elsevier, 2010. http://dx.doi.org/10.1016/b978-0-12-374420-3.00086-3.

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Conference papers on the topic "Oleanic acid"

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Liu, Shu Ang, Yulian Zhang, Zhen Zhou, Sijia Guo, Wanshan Song, Linlin Zhang, and Taotao Tian. "Oleanolic acid on SAMP8 mice in vivo experimental intervention study." In 2012 International Symposium on Information Technology in Medicine and Education (ITME 2012). IEEE, 2012. http://dx.doi.org/10.1109/itime.2012.6291419.

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Khalid, Sopiah Ambong, Marshahida Mat Yashim, Zarina Omar, Mohd Shahrul Nizam bin Salleh, Noraini Razali, Norkamruzita Saadon, and Norzila Mohd. "Identification of oleanolic and ursolic acid in aquatic plant Cabomba furcata." In 2013 IEEE Business Engineering and Industrial Applications Colloquium (BEIAC). IEEE, 2013. http://dx.doi.org/10.1109/beiac.2013.6560117.

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Santos, Raquel, Pedro L. Silva, Gisele P. Oliveira, Fernanda F. Cruz, Débora S. Ornellas, Manuela Lanzetti, Janaina Fernandes, Samuel Valença, Cerli R. Gattass, and Patricia R. M. Rocco. "Understanding The Mechanism Of Action Of Oleanolic Acid In Experimental Acute Lung Injury." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a2156.

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Jung, J. W., E. Jeong, K. Hwang, C. Park, H. Lim, and H. R. Kim. "Oleanolic Acid Acetate Alleviates Bleomycin-Induced Pulmonary Fibrosis Via Inhibiting Epithelial-Mesenchymal Transition." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a1522.

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Wu, Xuewen, and Yan Xiong. "Effects of NaCl and pH on 2-hydroxypropyl-beta-cyclodextrin inclusion with oleanolic acid." In 2011 International Conference on Human Health and Biomedical Engineering (HHBE). IEEE, 2011. http://dx.doi.org/10.1109/hhbe.2011.6028044.

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Kim, Hak-Ryul, Ki-Eun Hwang, Chul Park, Jae-Wan Jung, and Eun-Taik Jeong. "Oleanolic acid acetate ameliorates bleomycin-induced pulmonary fibrosis through inhibition of epithelial-mesenchymal transition." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa5380.

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Antoniadi, L., A. Angelis, D. Michailidis, and LA Skaltsounis. "Rapid and effective recovery of oleanolic and maslinic acid from olive products using centrifugal partition extraction." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399931.

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Knieper, Louis H., Ron Kalewski, and Jeffrey Carlson. "Determination of oleanolic acid based saponin removal by thewastewater treatment system at Southern Minnesota Beet Sugar Cooperative." In American Society of Sugarbeet Technologist. ASSBT, 2011. http://dx.doi.org/10.5274/assbt.2011.75.

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Wang, Xiuying, Wei Zhang, Chunlin Zhao, and Yang Chen. "Research on Platycodon Grandiflorum Eriobotrya Japonica Honey Tablet Technology and Determination of Content of Platycodin D, Ursolic and Oleanolic Acid." In 2017 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/icmmcce-17.2017.26.

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Narożna, Maria, Violetta Krajak-Kuźniak, Robert Kleszcz, and Wanda Maria Baer-Dubowska. "Abstract 127: Oleanolic acid oxime derivatives modulate NF-κB signaling pathway leading to cell cycle arrest in pancreatic cancer cells." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-127.

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