Relevant bibliographies by topics / Oligonucleotide Oligonucleotide

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Academic literature on the topic 'Oligonucleotide Oligonucleotide'

Author: Grafiati
Published: 4 June 2021
Last updated: 19 February 2022

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Journal articles on the topic "Oligonucleotide Oligonucleotide"

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Dung, Tran Huu, Seung-Rok Lee, Suhk-Dong Han, et al. "Chitosan-TPP Nanoparticle as a Release System of Antisense Oligonucleotide in the Oral Environment." Journal of Nanoscience and Nanotechnology 7, no. 11 (2007): 3695–99. http://dx.doi.org/10.1166/jnn.2007.041.

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Antisense oligonucleotide loaded chitosan nanoparticles were prepared and the release of oligonucleotide from chitosan-TPP/oligonucleotide nanoparticles was investigated. Morphological property, zeta potential and particle size of the prepared chitosan/oligonucleotide nanoparticles were investigated using Field Emission-Scanning Electron Microscope (FE-SEM) and particle size analyzer. The interaction between chitosan and oligonucleotide was confirmed by using capillary zone electrophoresis (CZE), and the released oligonucleotides were determined by spectrophotometric method. Oligonucleotides formed the complexes with chitosan with a unique morphological property. The release of oligonucleotides from nanoparticles was dependent on loading methods and pH conditions. Chitosan/oligomer-TPP nanoparticles, which was prepared by adding TPP after the formation of chitosan/oligonucleotide complex, showed the lowest release percent of oligonucleotides with 41.3% at pH 7.0 among the loading methods. The percent release of oligonucleotide from oligonucleotide loaded chitosan nanoparticle at pH 10 was higher than the one in acidic condition (pH 5.0). The released oligonucleotides from chitosan/oligonucleotide nanoparticles were stable enough for 12 h under the 20% saliva solution. Our results suggest that the sustained release of oligonucleotide from chitosan nanoparticles may be suitable for the local therapeutic application in periodontal diseases.
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Juliano, Rudolph L. "Chemical Manipulation of the Endosome Trafficking Machinery: Implications for Oligonucleotide Delivery." Biomedicines 9, no. 5 (2021): 512. http://dx.doi.org/10.3390/biomedicines9050512.

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Antisense oligonucleotides (ASOs), siRNA and splice switching oligonucleotides (SSOs) all have immense potential as therapeutic agents, potential that is now being validated as oligonucleotides enter the clinic. However, progress in oligonucleotide-based therapeutics has been limited by the difficulty in delivering these complex molecules to their sites of action in the cytosol or nucleus of cells within specific tissues. There are two aspects to the delivery problem. The first is that most types of oligonucleotides have poor uptake into non-hepatic tissues. The second is that much of the oligonucleotide that is taken up by cells is entrapped in endosomes where it is pharmacologically inert. It has become increasingly recognized that endosomal trapping is a key constraint on oligonucleotide therapeutics. Thus, many approaches have been devised to address this problem, primarily ones based on various nanoparticle technologies. However, recently an alternative approach has emerged that employs small molecules to manipulate intracellular trafficking processes so as to enhance oligonucleotide actions. This review presents the current status of this chemical biology approach to oligonucleotide delivery and seeks to point out possible paths for future development.
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Ravnik, Šimen, Ines Žabkar, Uršula Prosenc Zmrzljak, et al. "OligoPrime: An Information System for Oligonucleotide Management." Biomedical Engineering and Computational Biology 12 (January 2021): 117959722110419. http://dx.doi.org/10.1177/11795972211041983.

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With the increasing number of molecular biology techniques, large numbers of oligonucleotides are frequently involved in individual research projects. Thus, a dedicated electronic oligonucleotide management system is expected to provide several benefits such as increased oligonucleotide traceability, facilitated sharing of oligonucleotides between laboratories, and simplified (bulk) ordering of oligonucleotides. Herein, we describe OligoPrime, an information system for oligonucleotide management, which presents a computational support for all steps in an oligonucleotide lifecycle, namely, from its ordering and storage to its application, and disposal. OligoPrime is easy to use since it is accessible via a web browser and does not require any installation from the end user’s perspective. It allows filtering and search of oligonucleotides by various parameters, which include the exact location of an oligonucleotide, its sequence, and availability. The oligonucleotide database behind the system is shared among the researchers working in the same laboratory or research group. Users might have different roles which define the access permissions and range from students to researchers and primary investigators. Furthermore, OligoPrime is easy to manage and install and is based on open-source software solutions. Its code is freely available at https://github.com/OligoPrime . Moreover, an implementation of OligoPrime, which can be used for testing is available at http://oligoprime.xyz/ . To our knowledge, OligoPrime is the only software solution dedicated specifically to oligonucleotide management. We strongly believe that it has a large potential to enhance the transparency of use and to simplify the management of oligonucleotides in academic laboratories and research groups.
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Studzińska, Sylwia, Ewelina Zawadzka, Szymon Bocian, and Michał Szumski. "Synthesis and application of stationary phase for DNA-affinity chromatographic analysis of unmodified and antisense oligonucleotide." Analytical and Bioanalytical Chemistry 413, no. 20 (2021): 5109–19. http://dx.doi.org/10.1007/s00216-021-03473-7.

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AbstractThe goal of the research was the synthesis and application of an oligonucleotide immobilized stationary phase for the analysis of unmodified and antisense oligonucleotides. The method for attaching these molecules to aminopropyl silica modified with pentanedioic acid was developed. Each step of the synthesis was carefully controlled with the application of spectroscopic, elemental, and chromatographic analyses. The oligonucleotide-based stationary phase was applied for the retention studies. Unmodified oligonucleotides of different complementarity to the molecule attached as a stationary phase, as well as antisense oligonucleotides, were tested. The comparative study upon complex optimization of oligonucleotide analysis in different liquid chromatography modes was performed. Results have shown that this stationary phase may be applied for oligonucleotide analysis in hydrophilic interaction liquid chromatography and ion exchange chromatography, but no unique sequence-based selectivity was obtained. Contrary results were observed for affinity chromatography, which allowed for specific separation of the complementary strands based on hydrogen bonding and stacking interactions, where the temperature was the main factor influencing the selectivity of the separation. Furthermore, the oligonucleotide-based stationary phase may be applied for comparative antisense oligonucleotide hybridization studies to a specific RNA sequence. All of the results have shown that affinity chromatography with oligonucleotide-based stationary phases is a powerful technique for the specific base recognition of polynucleotides. Graphical abstract
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Milton, S., M. Murtola, J. Sandbrink, E. Yeheskiely, and R. Stromberg. "Making Oligonucleotide Conjugates and Breaking Oligonucleotides." Nucleic Acids Symposium Series 51, no. 1 (2007): 61. http://dx.doi.org/10.1093/nass/nrm031.

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Katsaloulis, P., T. Theoharis, and A. Provata. "Statistical Algorithms for Long DNA Sequences: Oligonucleotide Distributions and Homogeneity Maps." Scientific Programming 13, no. 3 (2005): 177–88. http://dx.doi.org/10.1155/2005/807304.

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The statistical properties of oligonucleotide appearances within long DNA sequences often reveal useful characteristics of the corresponding DNA areas. Two algorithms to statistically analyze oligonucleotide appearances within long DNA sequences in genome banks are presented. The first algorithm determines statistical indices for arbitrary length oligonucleotides within arbitrary length DNA sequences. The critical exponentμof the distance distribution between consecutive occurrences of the same oligonucleotide is calculated and its value is shown to characterize the functionality of the oligonucleotide. The second algorithm searches for areas with variable homogeneity, based on the density of oligonucleotides. The two algorithms have been applied to representative eucaryotes (the animalMus musculusandthe plantArabidopsis thaliana) and interesting results were obtained, confirmed by biological observations. All programs are open source and publicly available on our web site.
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Day, P. J., P. S. Flora, J. E. Fox, and M. R. Walker. "Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency." Biochemical Journal 278, no. 3 (1991): 735–40. http://dx.doi.org/10.1042/bj2780735.

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Immobilization of oligonucleotides containing 5′-terminal thiol groups on thiol-terminated paramagnetic Biomag beads via disulphide bond formation was investigated. Oligonucleotides are demonstrated to couple at high yields, the linkage is stable at 90 degrees C and is reversible, and the immobilized oligonucleotide is available for complementary, but not non-complementary, hybridization. Specific hybridization capacity per micrograms of immobilized oligonucleotide exceeds that achieved with other forms of immobilization chemistries employing random attachment and/or specific end attachment of the oligonucleotide to the solid support. Adsorption of DNA on the surface of the beads was decreased by incubation in 0.2% SDS; other non-specific blocking agents had no effect. Brief heating of the beads possessing immobilized oligonucleotides at 90 degrees C before hybridization increased the amount of specific hybridization dependent upon the inclusion of poly(dT) spacer sequences 5′ to the immobilized oligonucleotide and 3′ to the thiol group. Increasing lengths of spacers [up to a poly(dT16) spacer] linearly increased hybridization of complementary sequences.
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Zhang, Hui Yong. "Solid-Phase Synthesis of DNA Chemical Sensor." Advanced Materials Research 815 (October 2013): 305–11. http://dx.doi.org/10.4028/www.scientific.net/amr.815.305.

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Oligonucleotides are essential components of many applications in molecular biology. The synthesis chemistry is robust and commercial oligonucleotide synthesizers have taken advantage of the chemistry to provide oligonucleotides of high quality and purity. This paper established nucleic acid synthesis platform to carry out the synthesis of the labeled nucleic acid probes based on the DNA synthesizer and solid-phase synthesis technology. We chose to study the automated synthesis starting from DMT protected FAM labeled amidite attached to controlled pore glass (CPG) support and the standard trityl-off oligonucleotide synthesis cycle was performed, yielding the solid-supported oligonucleotide. The reported automated solid-phase oligonucleotide synthesis procedure successfully employs the common iterative synthesis, deblocking, activation, coupling, capping, oxidation, and isolation steps in standard oligonucleotide synthesis. The automated synthetic approach can also be applied to oligonucleotides of different length, composition of nucleotide, demonstrating the universality of the method. Moreover, the synthesis involved the use of commercially available, safe, stable, and inexpensive reagents, particularly advantageous and attractive for their use in automated solid-phase synthesis. The synthesis allows custom tailoring of their structure to the requirements of biological assays within hours, as opposed to traditional approaches that require weeks or months of work in the laboratory. Therefore it will become much easier to investigate biological interactions and optimize for objectives such as the receptor mediated targeting of oligonucleotides.
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Kilanowska, Anna, Łukasz Nuckowski, and Sylwia Studzińska. "Studying in vitro metabolism of the first and second generation of antisense oligonucleotides with the use of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry." Analytical and Bioanalytical Chemistry 412, no. 27 (2020): 7453–67. http://dx.doi.org/10.1007/s00216-020-02878-0.

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Abstract The aim of the present investigation was the analysis and identification of antisense oligonucleotide metabolism products after incubation with human liver microsomes regarding four different oligonucleotide modifications. Separation and detection methods based on the use of liquid chromatography coupled with quadrupole time-of-flight mass spectrometry were developed for this purpose. Firstly, the optimization of mass spectrometer parameters was done to select those which ensure the highest possible sensitivity of oligonucleotide analysis. This step was conducted for two chromatographic modes—ion pair chromatography and hydrophilic interaction liquid chromatography—due to their common application in oligonucleotide analysis. Based on sensitivity results, ion pair chromatography coupled with mass spectrometry was selected for the separation of model oligonucleotide mixtures in order to verify its selectivity for N-deleted metabolite separation. Next, the developed method was applied in the examination of oligonucleotides in vitro metabolism. First, wide optimization of incubation parameters was conducted including the concentration of the reaction buffer components. Obtained results indicated that both 3′-exonucleases and 5′-exonucleases contributed to the biotransformation of oligonucleotides. Moreover, it may be concluded that the number of metabolites depends on oligonucleotide modification and consequently its resistance to enzymatic attack. Thus, the number of the oligonucleotide metabolites decreased with the decrease of the resultant polarity of oligonucleotide caused by chemical modification.
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Zhao, Q., X. Song, T. Waldschmidt, E. Fisher, and AM Krieg. "Oligonucleotide uptake in human hematopoietic cells is increased in leukemia and is related to cellular activation." Blood 88, no. 5 (1996): 1788–95. http://dx.doi.org/10.1182/blood.v88.5.1788.1788.

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Abstract The use of antisense oligonucleotides as tools for modulating gene expression represents a novel strategy for designing drugs to treat a variety of diseases. Several factors, including cellular uptake and internalization of the oligonucleotides, are important parameters in determining the effectiveness of antisense agents such as therapeutic drugs. We have studied oligonucleotides uptake in normal and leukemic human hematopoietic cells, such as peripheral blood, bone marrow (BM), and HL-60 cell line; and have found that, in normal human blood and BM, myeloid cells and B cells preferably took up more oligonucleotides than T cells. There was no marked difference in oligonucleotide uptake between CD4+ helper T cells and CD8+ cytolytic T cells. Leukemic cells had greater oligonucleotide uptake than their normal counterparts. Furthermore, oligonucleotide uptake was closely related to cell activation status and can be modulated by growth factors or inhibitors. These studies provide a basis for using oligonucleotides as therapeutic drugs both in vitro and in vivo.
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Dissertations / Theses on the topic "Oligonucleotide Oligonucleotide"

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Scotson, James L. "Understanding oligonucleotide synthesis." Thesis, University of Huddersfield, 2016. http://eprints.hud.ac.uk/id/eprint/32090/.

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Oligonucleotides are synthesised almost exclusively via the solid-supported phosphoramidite method. However popular this method may be, the expensive reagents used in large excess during the synthesis as well as the large amounts of organic and aqueous solvents and purification steps makes the scale-up of oligonucleotide synthesis costly and possibly harmful to the environment. The therapeutic use of anti-sense oligonucleotides (ASOs) is hindered by their susceptibility to nuclease catalysed hydrolysis and to overcome this problem ASOs have been modified commonly by the introduction of a phosphorothioate backbone. This research aims to provide a better understanding of some of the more problematic stages of the synthesis cycle, the formation of the sulfurizing agent and sulfurisation of inter-nucleotide phosphite linkages, in order to make this method more sustainable and efficient. The investigation of the activation, alcoholysis and hydrolysis of the phosphoramidites 2´-methoxy-5´-O-DMT-uridine 3´-CE phosphoramidite (UAm) and di-tert-butyl N,N-di-isopropyl phosphoramidite (DBAm) using several tetrazole activators found that complete conversion of the phosphoramidite UAm to products required an excess of activator and that this was due to the generation of di-isopropyl amine during coupling. Conductivity measurements show that the amine deprotonates the acidic activator and that the ammonium and tetrazolide ions that are subsequently formed strongly ion pair (Kip = 6540 M-1) removing free activator from solution. The tetrazole-catalysed reaction of phosphoramidites with oxygen nucleophiles was found to be first order with respect to phosphoramidite and activator and the nucleophilic displacement of the di-isopropyl amine group by the tetrazoyl group at phosphorus is rate-limiting. Investigation into the 3-picoline-catalysed ageing of the sulfur transfer reagent phenylacetyl disulfide (PADS) has shown that the process is overall second order and is proportional to the concentration of PADS and 3-picoline. Deuterium exchange experiments show that ageing proceeds via abstraction of the methylene CH2 protons of PADS via an E1cB-type decomposition of the PADS molecule generating a disulfide anion and a ketene by-product which was trapped using an intra-molecular [2+2]-cycloaddition reaction. Mass spectrometry data shows that disulfide anions act as nucleophiles with PADS molecules to generate polysulfides which are the active sulfur transfer reagents in aged PADS solutions. Using pyridines that are less basic than 3-picoline causes the rate of degradation of PADS to become slower, indicating the possibility that the rate-limiting step of this process is the generation of the disulfide anion. The rate of sulfurisation of phosphites by both ‘fresh’ and ‘aged’ PADS in the presence of 3-picoline was found to be first order with respect to phosphite, PADS and 3-picoline at low concentrations of each. However, the rate of the reaction becomes independent of base when using aged PADS in the presence of high 3-picoline concentration. Brönsted correlations for the sulfurisation of alkyl phosphites using fresh PADS give a βnuc value of 0.51, consistent with a mechanism involving nucleophilic attack by the phosphite on the disulfide bond of PADS to generate a phosphonium ion intermediate. This degrades to the phosphorothioate product via a base-catalysed mechanism which has been confirmed by removal of the methylene protons from the PADS molecule. Comparison of the βnuc values seen when altering the pKa of the pyridine catalyst used shows that the rate of the reaction of fresh PADS is much more sensitive to the pKa of the pyridine than is aged PADS (βnuc = 0.43 and 0.26 for fresh and aged PADS respectively). This suggests that in the case of aged PADS, the phosphite attacks the sulfur atom adjacent to the carboxylate group in the polysulfide chain. This generates a phosphonium intermediate which can be broken down via a much more facile S-S bond fission, as opposed to the C-S bond fission as seen in when using fresh PADS.
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Barnekow, Frank. "Darstellung einzelsträngiger DNA-Polymeraseprodukte über eine kovalente Membrananbindung und deren enzymatische Umsetzungen." [S.l. : s.n.], 1998. http://deposit.ddb.de/cgi-bin/dokserv?idn=957430485.

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Bauer, Oliver. "Technology development for oligonucleotide fingerprinting applying multiplexed PNA hybridizations and MALDI-TOF mass spectrometry detection /." [S.l. : s.n.], 2003. http://www.diss.fu-berlin.de/2003/326/index.html.

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Torres, Adrian Gabriel. "MicroRNA targeting with oligonucleotide analogues." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610040.

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Steck, Anna-Lena [Verfasser]. "Oligonucleotide-modified Nuclotides / Anna-Lena Steck." Konstanz : Bibliothek der Universität Konstanz, 2013. http://d-nb.info/1058325981/34.

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Luchi, Daniela de. "Crystallographic study on oligonucleotide coiled-coils." Doctoral thesis, Universitat Politècnica de Catalunya, 2008. http://hdl.handle.net/10803/6457.

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En la presente tesis doctoral se han realizado estudios estructurales de DNA. Estudios previos han demostrado que los coiled-coils de d(ATATATATATAT) y d(ATATATATAT) tienen unos parámetros geométricos muy diferentes. El objetivo de esta tesis es aclarar las propiedades de los coiled-coils.<br/>Con esta finalidad se han estudiado por cristalografía de Rayos X oligonucleótidos con diferentes secuencias y con extremos cohesivos que fijen la geometría de los coiled-coils. Se han utilizado oligonucleótidos con la secuencia d(CG)n(AT)m o (AT)m(CG)n y otros semejantes. En la mayor parte de ellos n=1 y m>1, con lo que el extremo cohesivo es normalmente la secuencia CG. <br/>La estructura (a una resolución de 3.1 Å) de los cristales generados por la secuencia d(CGATATATATAT) ha sido resuelta y publicada (De Luchi et al., ChemBiochem 2006, 7, 585-587). La estructura es isomorfa con la estructura de d(AT)6 y los enlaces a puente de hidrogeno entre las bases A y T son de tipo Hoogsteen, como en el caso de la secuencia d(ATATAT). Se han obtenido diferentes tipos de coiled-coils y se han estudiado sus características y propiedades.<br/>Hemos analizado las propiedades geométricas de los coiled coils: hemos visto que los parámetros que determinan el numero de oligonucleótidos por vuelta son el "kink angle" &#952;, y el ángulo de torsión &#964;. Ha sido estudiada la relación entre estos parámetro, en función del numero de oligonucleótidos por vuelta N y el ángulo de inclinación &#946; del coiled-coil.<br/>Hemos intentado determinar si la formación de apareamientos de tipo Hoogsteen puede influir en la geometría de los coiled-coils, los resultados sugieren que los puentes de hidrogeno de tipo Hoosteeen favorecen la formación de coiled-coils, mientras los enlaces de tipo Watson-Crick generan mas fácilmente estructuras estándar de DNA pseudocontinuas. La secuencia d(CGATATGCATAT) genera columnas tradicionales de DNA, las bases centrales G y C, apareándose con enlaces Watson-Crick fuerzan las bases ATs a aparearse de la misma manera, generando así una hélice recta pseudocontinua.<br/>Como complemento de estos estudios se han obtenido las curvas de fusión de oligonucleótidos ricos en AT, los resultados, que incluyen una formula que permite un calculo aproximado de la temperatura de fusión de secuencias cortas de DNA, han sido publicados (De Luchi et al., Analytical Biochemistry, 2003, 322, 279-282).<br/>También he tenido la oportunidad de refinar la estructura de d(TAGG) en complejo con un derivado antraquinonico que ya había sido resuelta en nuestro laboratorio. Inesperadamente, encontramos que en esta estructura no se forman G-cuádruplex como fue descrito en solución por Kettani et al, las moléculas de fármaco, a través de interacciones de stacking forman un retículo de columnas perpendiculares una a la otra, estabilizado en los "crossing points" por las flexibles y cortas moléculas de DNA. La flexibilidad del DNA gracias a sus siete ángulos de torsión, le permite adoptar una conformación tal que se adapta a la estructura creada por las columnas de fármaco. La capacidad de formar diferentes tipos de enlaces a puente de hidrogeno estabiliza su conformación en este caso no canónica. En esta estructura hemos encontrado los siguientes tipos de enlaces a puente de hidrogeno: estándar Watson-Crick, reverse Watson-Crick, interacciones simétricas Guanina-Guanina.
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Pritchard, Clare Elizabeth. "Studies in solid supported oligonucleotide synthesis." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/11282.

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Wolfe, Justin Mahoney. "Peptide conjugation to enhance oligonucleotide delivery." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/118278.

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Thesis: Ph. D. in Biological Chemistry, Massachusetts Institute of Technology, Department of Chemistry, 2018.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>The intracellular delivery of functional macromolecules remains an outstanding challenge in biomedicine. While small molecules can diffuse through the plasma membrane, many large therapeutic molecules are not internalized to an appreciable extent. One strategy to improve cell uptake involves linking the molecule of interest to a cell-penetrating peptide (CPP). CPPs are widely employed to enhance macromolecule delivery, with hundreds of different peptides and modifications reported to improve cellular uptake. In this thesis, CPPs were systematically investigated and chemically altered to facilitate the delivery of antisense oligonucleotides. To accurately compare the existing CPPs, 64 CPP sequences were synthesized, conjugated to oligonucleotides, and assayed for delivery. These CPPs showed a range of effectiveness, with some CPPs hindering the delivery of oligonucleotide cargo and others leading to a 10-fold increase in oligonucleotide activity. To help identify which CPPs might be valuable for oligonucleotide delivery specifically, a computational model was developed to predict, de novo, whether or not a CPP will be effective. When experimentally validated, this model successfully predicted which sequences would improve oligonucleotide delivery greater than 3-fold. Multiple strategies were employed to improve CPP effectiveness. First, arginine-rich CPPs were chemically modified with perfluoroarenes. Cyclic and bicyclic CPPs were synthesized by linking multiple cysteine residues together with a perfluoroarene. After oligonucleotide conjugation, these peptides led to a 14-fold increase in delivery. Second, two different CPPs were combined into one long chimeric sequence. The CPP chimeras were highly active, leading to a 20-fold increase in oligonucleotide delivery. Third, the idea of combining multiple CPPs led to the development of a method for the rapid synthesis combinatorial peptide conjugates. Using the judicious choice of bioconjugation chemistry, highly-active modular constructs were synthesized that contain three peptides linked to one oligonucleotide. In addition to CPPs for oligonucleotide delivery, one section of this thesis employed perfluoroaryl macrocyclic peptides to address the challenge of peptide delivery across the blood-brain barrier. An additional section developed a new peptide conjugation strategy that uses palladium-peptide oxidative addition complexes as solid, storable, and water-soluble reagents for bioconjugation.<br>by Justin Mahoney Wolfe.<br>Ph. D. in Biological Chemistry
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Razumovitch, Julia. "Hybridization of surface-tethered oligonucleotide brushes /." [S.l.] : [s.n.], 2009. http://edoc.unibas.ch/diss/DissB_8744.

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Douglas, Andrew Graham Lim. "Oligonucleotide-based therapies for neuromuscular disease." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:d14706a3-c436-46ff-87c4-40bbbad6dc01.

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Books on the topic "Oligonucleotide Oligonucleotide"

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Thomas, Tuschl, Rossi John J, and New York Academy of Sciences, eds. Oligonucleotide therapeutics. Blackwell on behalf of the New York Academy of Sciences, 2006.

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Herdewijn, Piet. Oligonucleotide Synthesis. Humana Press, 2004. http://dx.doi.org/10.1385/1592598234.

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Gissberg, Olof, Rula Zain, and Karin E. Lundin, eds. Oligonucleotide-Based Therapies. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9670-4.

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Agrawal, Sudhir. Protocols for Oligonucleotide Conjugates. Humana Press, 1994. http://dx.doi.org/10.1007/978-1-59259-513-6.

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Agrawal, Sudhir. Protocols for Oligonucleotide Conjugates. Humana Press, 1993. http://dx.doi.org/10.1385/0896032523.

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J, Rossi John, Gait, M. J. (Michael J.), Eckstein Fritz 1932-, and New York Academy of Sciences, eds. Oligonucleotide therapeutics: Fourth annual meeting. Published by Blackwell Pub. on behalf of the New York Academy of Sciences, 2009.

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Ferrari, Nicolay, and Rosanne Seguin, eds. Oligonucleotide-Based Drugs and Therapeutics. John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781119070153.

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Hyman, Edward David. The Hyman method: Oligonucleotide synthesis and plasmid preparation. Sybtrel Biotechnology, 1995.

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Tiling arrays: Methods and protocols. Humana Press, 2013.

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He, Zhili. Microarrays: Current technology, innovations and applications. Caister Academic Press, 2014.

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Book chapters on the topic "Oligonucleotide Oligonucleotide"

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Kawamura, Kunio. "Oligonucleotide." In Encyclopedia of Astrobiology. Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27833-4_1105-3.

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Kawamura, Kunio. "Oligonucleotide." In Encyclopedia of Astrobiology. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-11274-4_1105.

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Kawamura, Kunio. "Oligonucleotide." In Encyclopedia of Astrobiology. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-44185-5_1105.

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Caruthers, Marvin H. "Synthesis of Oligonucleotides and Oligonucleotide Analogues." In Oligodeoxynucleotides. Macmillan Education UK, 1989. http://dx.doi.org/10.1007/978-1-349-10869-5_2.

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Macaulay, Val. "Oligonucleotide therapies." In Molecular Biology for Oncologists. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-3111-5_26.

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Denti, Michela Alessandra, and Giuseppina Covello. "Oligonucleotide Therapy." In Safety and Efficacy of Gene-Based Therapeutics for Inherited Disorders. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-53457-2_9.

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Stein, Cy A., Britta Hoehn, and John Rossi. "Oligonucleotide Therapeutics." In Principles of Anticancer Drug Development. Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-7358-0_20.

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Gawad, Shady, Ana Valero, Thomas Braschler, et al. "Oligonucleotide Amplification." In Encyclopedia of Nanotechnology. Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-90-481-9751-4_100603.

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Kong, Deming. "Oligonucleotide Probes." In Advanced Topics in Science and Technology in China. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-34303-2_13.

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Ruth, Jerry L. "Oligonucleotide-Enzyme Conjugates." In Protocols for Oligonucleotide Conjugates. Humana Press, 1994. http://dx.doi.org/10.1007/978-1-59259-513-6_6.

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Conference papers on the topic "Oligonucleotide Oligonucleotide"

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McGall, Glenn H., and Jacqueline A. Fidenza. "High-density oligonucleotide probe arrays." In BiOS 2000 The International Symposium on Biomedical Optics, edited by Patrick A. Limbach, John C. Owicki, Ramesh Raghavachari, and Weihong Tan. SPIE, 2000. http://dx.doi.org/10.1117/12.380501.

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Shao, Li, Shaoqiang Chen, Jian Zhang, Jianzhong Zhu, Ziqiang Zhu, and Bin Zhu. "Porous-silicon-based oligonucleotide chips." In SPIE Proceedings, edited by Junhao Chu, Zongsheng Lai, Lianwei Wang, and Shaohui Xu. SPIE, 2004. http://dx.doi.org/10.1117/12.607477.

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Chmielewski, Marcin K. "Thermolabile protecting groups in oligonucleotide synthesis." In XVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201112317.

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Azhayev, Alex. "Universal solid supports for oligonucleotide synthesis." In XIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 1999. http://dx.doi.org/10.1135/css199902129.

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Kwiatkowski, Marek, Simon Fredriksson, Anders Isaksson, Mats Nilsson, and Ulf Landegren. "In situ reversal of oligonucleotide orientation." In XIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 1999. http://dx.doi.org/10.1135/css199902213.

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RAVELET, CORINNE, ERIC DAUSSE, ERIC PEYRIN, GUILLAUME DURAND, and JEAN JACQUES TOULME. "Sensing small molecules with oligonucleotide aptamers." In Second International Conference on Advances in Bio-Informatics and Environmental Engineering - ICABEE 2015. Institute of Research Engineers and Doctors, 2015. http://dx.doi.org/10.15224/978-1-63248-043-9-80.

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Sun, Jian, and Brian M. Cullum. "SERS beacons for multiplexed oligonucleotide detection." In Optics East 2007, edited by Brian M. Cullum and D. Marshall Porterfield. SPIE, 2007. http://dx.doi.org/10.1117/12.730450.

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Trulson, Mark O., David Stern, Ian D. Walton, Audrey D. Suseno, and Richard P. Rava. "Fluorescence microscopy of oligonucleotide probe arrays." In BiOS '97, Part of Photonics West, edited by Richard B. Thompson. SPIE, 1997. http://dx.doi.org/10.1117/12.273511.

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Brown, Paige K., Ammar T. Qureshi, Daniel J. Hayes, and W. Todd Monroe. "Targeted Gene Silencing With Light and a Silver Nanoparticle Antisense Delivery System." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53647.

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Abstract:
Targeted delivery and controlled release of oligonucleotide therapeutics in vivo are essential aspects of an ideal delivery vehicle. Here we demonstrate the synthesis and in vitro/intracellular characterization of silver nanoparticle (SNP) photolabile nucleic acid conjugates, with the aim of developing a nanoparticulate platform for inducible gene silencing. Due to unique size related properties, nanostructures are being increasingly utilized for intracellular diagnostics and delivery applications. While most nanoscale delivery platforms are polymeric in composition, studies of metallic nanoparticles have highlighted their suitability for delivery of therapeutic agents such as antisense oligonucleotides [1]. The potential benefits of noble metal nanoparticles in delivery applications include tunable size and shape, ease of bulk synthesis and functionalization via ‘wet chemistry’ techniques, and enhanced stability of tethered DNA [2]. Silver is one of the best surface-enhancing substrates available for nanostructure synthesis [3]. SNP composites afford external control over surface-tethered drug release via external triggers.
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Kostov, Ondřej, Eva Zborníková, Miloš Buděšínský, Pavel Novák, and Ivan Rosenberg. "Sulfur-containing phosphonate monomers for oligonucleotide synthesis." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414308.

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Reports on the topic "Oligonucleotide Oligonucleotide"

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Miller, E., R. Mariella, Jr, A. Christian, S. Gardner, and J. Williams. Oligonucleotide and Long Polymeric DNA Encoding. Office of Scientific and Technical Information (OSTI), 2003. http://dx.doi.org/10.2172/15009752.

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Wong, Ho-Lun. Megalin-Mediated Oligonucleotide Trafficking for Breast Cancer Chemosensitization. Defense Technical Information Center, 2010. http://dx.doi.org/10.21236/ada542623.

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Cohen, Jack S. Inhibition of Gene Expression by Alkylphosphonate Oligonucleotide Analogs with RNase-Like Activity. Defense Technical Information Center, 1989. http://dx.doi.org/10.21236/ada207296.

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Kemp, P. F., S. Lee, and J. LaRoche. Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes. Office of Scientific and Technical Information (OSTI), 1992. http://dx.doi.org/10.2172/6973949.

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Kemp, P. F., S. Lee, and J. LaRoche. Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes. Office of Scientific and Technical Information (OSTI), 1992. http://dx.doi.org/10.2172/10181975.

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Pirrung, M. C. Preparation of oligonucleotide arrays for hybridization studies: Final report, 2/15/92-5/4/96. Office of Scientific and Technical Information (OSTI), 1996. http://dx.doi.org/10.2172/486589.

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Bates, Paula J. Mechanism of Action of Novel Antiproliferative Oligonucleotides. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada406133.

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Jing, Naijie. Targeting Stat3 With G-Quartet Oligonucleotides in Metastatic Prostate Cancer. Defense Technical Information Center, 2005. http://dx.doi.org/10.21236/ada438389.

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Jing, Naijie. Targeting Stat3 With G-Quartet Oligonucleotides in Metastatic Prostate Cancer. Defense Technical Information Center, 2004. http://dx.doi.org/10.21236/ada426083.

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Jing, Naijie. Targeting Stat3 With G-Quartet Oligonucleotides in Metastic Prostate Cancer. Defense Technical Information Center, 2006. http://dx.doi.org/10.21236/ada455577.

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