Relevant bibliographies by topics / Oocyte. Ovum

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  2. Dissertations / Theses
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Academic literature on the topic 'Oocyte. Ovum'

Author: Grafiati
Published: 4 June 2021
Last updated: 2 February 2022

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Journal articles on the topic "Oocyte. Ovum"

1

Techakumphu, M., A. Promdireg, N. Phutikanit, et al. "339 ULTRASOUND GUIDED OVUM PICK UP (OPU) IN PREPUBERTAL SWAMP BUFFALO USING THREE DIFFERENT VACUUM PRESSURES." Reproduction, Fertility and Development 17, no. 2 (2005): 320. http://dx.doi.org/10.1071/rdv17n2ab339.

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Our group successfully developed ovum pick-up in prepubertal swamp buffalo, however the quality of the oocytes that were collected was poor especially those without a cumulus mass (Techakumphu et al. 2003 Theriogenology 61, 1705–1711). The vacuum pressure used for oocyte collection was one of the factors influencing oocyte quality (Bols et al. 1997 Theriogenology 47, 1221–1236). The objective of this study was to compare the effect of three vacuum pressures on both the recovery rate and oocyte quality in prepubertal swamp buffaloes. Oocyte recovery and oocyte quality were using different groups of aspiration vacuum pressures. The maturation stages of the recovered oocytes were immediately assessed by fixation and rapid staining with basic carbol fuchsin. Twelve prepubertal calves, aged 1.5 yrs were a total of 180 mg FSH given, twice a day, in divided doses over 3 d (40/40, 30/30, 20/20). The animals were randomized into 3 groups, according to the different vacumm pressures, 100 (n = 8), 80 (n = 8) and 60 mmHg (n = 8). Two sets of treatments, carried out, with a 2 month interval between them. The oocyte recovery rates using 100 and 80 mmHg, were not different at 78.4% (29/37) and 83.6% (61/73). The 60 mmHg gave a lower rate, 65.7% (23/35) which was statistically different from the 80 mmHg group (P < 0.05). The oocytes recovered per donor showed no significant difference among the groups; 5.8 ± 4.9 for 100 mmHg, 7.6 ± 8.6 for 80 mmHg and 3.3 ± 2.1 for 60 mmHg respectively. The percentage of cumulus-oocyte complex (COC), single layered+partial cumulus oocytes (S + P) and expanded cumulus oocytes (EXP) showed no differences for any of the pressures, being 79.3, 65.5 and 82% of the total recovered. The experiment showed that follicles with a size of 2–6 mm were dominant after FSH treatment, being around 80% of the total number. Furthermore, the maturation stages of these oocytes were at prophase I and metaphase I. In conclusion, the vacuum pressure used for the oocyte retrieval technique influenced the recovery rate but not the oocyte quality. This study was supported by Rajadapisek Sompoj Fund, Chulalongkorn University year 2002. AP was PhD candidate under Royal Golden Jubilee, Thailand Research Fund.
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Silva, Bárbara Letícia Marchi da, Paulo Roberto Adona, Samuel Guemra, Paulo Sergio Monzani, and Moysés dos Santos Miranda. "Ovum Pick Up: Cows Treated With Single Doses of Follicle Stimulating Hormone." Journal of Agricultural Science 11, no. 10 (2019): 231. http://dx.doi.org/10.5539/jas.v11n10p231.

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Cows treated with single doses of follicle stimulating hormone (FSH) and ovum pick up (OPU) after 24 hours were evaluated for oocyte recovery, in vitro production of embryos (IVPE), and transferred embryos. To begin evaluations, the ovarian follicles larger than three millimeters in diameters were removed from all cows used in the study. Two days after OPU, 200 milligrams of FSH was given in a single dose in 6 cows (treated). Twenty-four hours after application of FSH, the cows underwent a new OPU session for oocyte retrieval. These procedures were repeated three consecutive times without interval. In control (FSH-free) cows the OPU were performed at intervals of one week or oocyte retrieval. The viable oocytes were submitted to IVPE, and the blastocysts were transferred to the recipients. The mean number of oocytes did not differ (p &gt; 0.05) between control cows (12.1&plusmn;2.8) and those treated (10.9&plusmn;1.6). There were also no differences (p &gt; 0.05) in the number (6.6&plusmn;1.7 and 7.1&plusmn;0.9, respectively) or in the percentage (54.4&plusmn;3.3 and 64.5&plusmn;3.1%, respectively) of viable oocytes between control cows and those treated. The percentage of IVPE and pregnancy did not differ (p &gt; 0.05) between control cows (39.8&plusmn;2.6% and 44.7&plusmn;4.8%) and those treated (37.8&plusmn;2.5% and 39.5&plusmn;4.1%), respectively. The treatment with single doses of FSH did not significantly alter the evaluated results for oocyte recovery, IVPE and transferred embryos, and, therefore, did not promote significant improvements for the IVPE as a whole.
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Hunter, M. "Oocyte maturation and ovum quality in pigs." Reviews of Reproduction 5, no. 2 (2000): 122–30. http://dx.doi.org/10.1530/revreprod/5.2.122.

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Hunter, M. "Oocyte maturation and ovum quality in pigs." Reviews of Reproduction 5, no. 2 (2000): 122–30. http://dx.doi.org/10.1530/ror.0.0050122.

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Foster, B. A., F. A. Diaz, E. J. Gutierrez, and K. R. Bondioli. "158 The Effect of Follicular Wave Phase at Time of Ovum Pick-Up on Bovine Oocyte Cytoplasmic Maturation and Developmental Competence." Reproduction, Fertility and Development 30, no. 1 (2018): 218. http://dx.doi.org/10.1071/rdv30n1ab158.

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During oocyte collection, follicular wave phase is unknown, although differences in follicle environment may have dramatic effects on oocyte quality. This project was performed to determine whether oocyte collection during different phases of the follicular wave affects oocyte competence. Oocytes were collected via transvaginal ultrasound guided oocyte aspiration from 18 cows, at 4, 8, and 12 days following dominant follicle removal, representing follicle wave emergence, peak, and atresia, respectively (160, 314, and 273 oocytes, respectively). Once recovered, oocytes were graded and assigned to either being held as immature or matured in vitro for 24 h. Oocytes were then stained in Mitotracker deep red, fixed and stained with an anti-IP3R1 primary antibody and an Alexa Fluor 488-conjugated secondary antibody, before being stained with DAPI, to identify mitochondria, inositol triphosphate receptor 1 (IP3R1), and chromatin respectively. Mitochondria were analysed based on cytoplasmic distribution and classified as peripheral (immature), diffuse, central (mature), or sparse. Expression of IP3R1 was measured as corrected total cell fluorescence in Image J (National Institutes of Health, Bethesda, MD, USA). Staining patterns were analysed using ANOVA. A subset of the matured oocytes was stained with Fluo-3 to measure cytoplasmic calcium levels. These oocytes were then parthenogenetically activated before being imaged again to view changes in calcium levels, and presumptive embryos were cultured for 4 days. Fluo staining was measured as intensity levels (none, slight, moderate, high) and differences in development and stain levels were analysed using the Kruskal-Wallis test. Although mitochondria location was unaffected by collection day, it was significantly affected by maturation status (P = 0.0036). However, oocytes showed incomplete mitochondrial maturation, with mitochondria residing in the diffuse orientation in the majority of oocytes. Expression of IP3R1 appeared to be more sensitive to treatment. Expression significantly increased as meiosis proceeded (P = 0.0081) and there was a significant difference in expression between oocyte collection days (P = 0.0026). The interaction between collection day and maturation status also had a significant effect (P = 0.048), with mature oocytes showing an increase in IP3R1 expression, most notable in those collected on Day 4. Oocyte quality had a notable effect on the ability of oocytes to progress through meiosis (P = 0.054) and on mitochondrial location (P = 0.053), with AB oocytes showing better maturation parameters in both respects. Although the day of collection did not affect embryo development, Fluo stain intensity was an indicator of embryo developmental potential (P = 0.053), with oocytes having decreased potential to develop if the initial calcium levels were moderate to high. Results suggest that oocyte collection during wave emergence yields a slight advantage in oocyte quality. Although IP3R1, necessary for Ca2+ spikes during fertilization, indicates competence, high levels of cytoplasmic Ca2+ at the time of activation appear to be detrimental to embryo development.
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Imai, K., M. Tagawa, S. Matoba, M. Narita, and N. Saito. "246 FOLLICULAR DEVELOPMENT AND OOCYTE QUALITY AFTER OVUM PICKUP IN DONOR COWS." Reproduction, Fertility and Development 17, no. 2 (2005): 273. http://dx.doi.org/10.1071/rdv17n2ab246.

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The present study was designed to assess the renewal of follicular development and oocyte quality after ovum pickup (OPU) in Holstein dry cows. Cows were kept under the same feeding and environmental conditions. In Experiment 1, follicle aspiration (more than 2 mm) by OPU using a 7.5 MHz linear transducer with needle (cova needle, Misawa Medical, Tokyo, Japan) connected to an ultrasound scanner (SSD-1200, ALOKA, Tokyo, Japan) was performed in four cows. After OPU ovaries were observed from Day 4 (Day 0 = the day of OPU) to Day 11 to assess the number of follicles that developed. In Experiment 2, two sessions of OPU (n = 11) were performed with a 7-day interval between to assess the quality of developing follicles and oocytes. Oocytes were evaluated by their cumulus cell morphology, cytoplasmic color, and density. To assess the developmental competence of oocytes, collected cumulus-oocyte complexes (COCs) were cultured for 20 h in TCM-199 supplemented with 5% calf serum (CS) in a microdroplet (volume was adjusted to 5 μL/oocyte) at 38.5°C under atmosphere of 5% CO2 in air. After maturation, the COCs were inseminated with frozen-thawed semen collected from the same ejaculation of a single bull. The fertilization was performed with BO solution as described by Imai et al. (2002 J. Vet. Med. Sci. 64(10), 887–891). The putative zygotes were then cultured in CR1aa supplemented with 5% CS under the same conditions as maturation culture for nine days. Embryo development was assessed by the cleavage rate on Day 2 and the blastocyst formation rate on Days 7 to 9 (the day of insemination = Day 0). Data were analyzed by ANOVA or Student's t-test. In Experiment 1, the mean number of developing follicles (larger than 2 mm in diameter) were increased from Day 4 to Day 11 (Day 4: 19.8 ± 10.0, Day 7: 32.5 ± 9.5; Day 11: 39.5 ± 10.7 (mean ± SD), respectively, P < 0.05). In Experiment 2, the mean number of developing follicles and collected oocytes on the day of OPU were significantly (P < 0.05) different between the first and second sessions (54.2 ± 12.4 and 40.8 ± 12.7, 45.7 ± 20.2 and 27.7 ± 8.7, respectively). The percentage of Grade 1 and 2 oocytes for the first session was significantly lower (P < 0.05) than those for the second session (59.1 ± 8.4 and 69.0 ± 11.8), and no significant differences were found within cleavage and blastocyst rates. The mean numbers of blastocysts obtained per session were 14.2 ± 8.9 and 9.7 ± 6.3 in the first and second sessions, respectively. These results indicate that populations of follicles were increased till Day 11 after OPU, and proportion of normal oocytes were increased in the renewal follicles.
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King, CM, and GT Kovacs. "Oocyte Donation: Review of Results." Reproduction, Fertility and Development 4, no. 6 (1992): 719. http://dx.doi.org/10.1071/rd9920719.

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This paper reviews the results of oocyte donation programmes around the world. Data on 1608 cycles of ovum donation were received from 11 clinics and medical centres. Significance among and between groups of donors and recipients was also assessed.
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Santos, R. M., M. Oliveira, C. G. B. Demetrio, J. H. Hasler, J. C. Fonseca, and D. G. B. Demetrio. "146 Single injection of follicle-stimulating hormone before ovum pickup in lactating Holstein donors: Oocyte recovery and embryo production." Reproduction, Fertility and Development 33, no. 2 (2021): 181. http://dx.doi.org/10.1071/rdv33n2ab146.

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Lactating donor cows frequently have decreased oocyte quality, lower fertilization rates, and impaired early embryonic development due to their lactational metabolic challenges. Synchronization and stimulation of follicular growth before ovum pickup (OPU) has been used to improve oocyte quality and consequently, embryo production. The objective of this study was to evaluate the effects of a single injection of FSH before OPU on oocyte recovery and embryo production in lactating Holstein donors. During June and July 2020, 22 lactating Holstein donors (open, 40 to 90 DIM, producing &gt;90 lbs of milk) from Ruann Dairy (Riverdale, CA) were randomly assigned to one of two treatments (crossover design). Donors did not receive any injections before OPU when assigned to the No FSH treatment. Treatment 1×FSH consisted of a single intramuscular (IM) injection of 140mg of FSH (Folltropin®, Vetoquinol) 36h after gonadotrophin-releasing hormone (GnRH; Fertagyl, Merck®, 129µg, IM). The FSH consisted of a 3.5-mL IM injection of 400mg of FSH diluted in 10mL of 0.5% hyaluronan (HA). OPU was performed 48 to 52h after FSH. All donors received both treatments on a 14-day interval. The recovered oocytes were fertilized with the same sexed female-sorted semen in both rounds (3 different sires were used). OPU, oocyte classification, IVM, IVF, and culture (IVC) were performed as described by Demetrio et al. (2020 Anim. Reprod. 17, e20200053). All oocytes went into IVM, except for degenerated occytes. The number of 4-cell (or more) embryos on Day 3 of IVC divided by the number of oocytes in IVC after IVF is defined as the cleavage rate. The number of blastocysts (early to hatched) on Day 7 of IVP divided by the number of oocytes in IVC after IVF is defined as the blastocyst rate. Poisson-normal (count data) and Logistic-normal (proportion data) models were used to analyse the data. Treatment, donor (random effect), and sire were included in the models. The results are summarised in Table 1. Oocyte recovery and embryo production were highly donor dependent. There were no differences in the number of recovered oocytes among treatments. Stimulation of the follicular growth before OPU with one single injection of FSH diluted in 0.5% HA 36h after GnRH improved oocyte quality, cleavage rates, blastocyst rates, embryo quality, and the total number of embryos per OPU in lactating Holstein donors. Table 1. Oocyte recovery, cleavage rate, and embryo production results Treatment OPU Oocytes per donor Grade 1 and 2 oocytes Cleavage rate (%) Blastocyst rate (%) Total embryos per OPU Grade 1 embryos per OPU No FSH 22 18.2 30a 69a 22a 3.4a 1.6a 1×FSH 22 17.5 34b 77b 34b 5.3b 3.3b a,bValues with different superscripts in the same column differ (at least P&lt;0.05).
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Santos, R. M., M. Oliveira, C. G. B. Demetrio, J. H. Hasler, J. C. Fonseca, and D. G. B. Demetrio. "146 Single injection of follicle-stimulating hormone before ovum pickup in lactating Holstein donors: Oocyte recovery and embryo production." Reproduction, Fertility and Development 33, no. 2 (2021): 181. http://dx.doi.org/10.1071/rdv33n2ab146.

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Lactating donor cows frequently have decreased oocyte quality, lower fertilization rates, and impaired early embryonic development due to their lactational metabolic challenges. Synchronization and stimulation of follicular growth before ovum pickup (OPU) has been used to improve oocyte quality and consequently, embryo production. The objective of this study was to evaluate the effects of a single injection of FSH before OPU on oocyte recovery and embryo production in lactating Holstein donors. During June and July 2020, 22 lactating Holstein donors (open, 40 to 90 DIM, producing &gt;90 lbs of milk) from Ruann Dairy (Riverdale, CA) were randomly assigned to one of two treatments (crossover design). Donors did not receive any injections before OPU when assigned to the No FSH treatment. Treatment 1×FSH consisted of a single intramuscular (IM) injection of 140mg of FSH (Folltropin®, Vetoquinol) 36h after gonadotrophin-releasing hormone (GnRH; Fertagyl, Merck®, 129µg, IM). The FSH consisted of a 3.5-mL IM injection of 400mg of FSH diluted in 10mL of 0.5% hyaluronan (HA). OPU was performed 48 to 52h after FSH. All donors received both treatments on a 14-day interval. The recovered oocytes were fertilized with the same sexed female-sorted semen in both rounds (3 different sires were used). OPU, oocyte classification, IVM, IVF, and culture (IVC) were performed as described by Demetrio et al. (2020 Anim. Reprod. 17, e20200053). All oocytes went into IVM, except for degenerated occytes. The number of 4-cell (or more) embryos on Day 3 of IVC divided by the number of oocytes in IVC after IVF is defined as the cleavage rate. The number of blastocysts (early to hatched) on Day 7 of IVP divided by the number of oocytes in IVC after IVF is defined as the blastocyst rate. Poisson-normal (count data) and Logistic-normal (proportion data) models were used to analyse the data. Treatment, donor (random effect), and sire were included in the models. The results are summarised in Table 1. Oocyte recovery and embryo production were highly donor dependent. There were no differences in the number of recovered oocytes among treatments. Stimulation of the follicular growth before OPU with one single injection of FSH diluted in 0.5% HA 36h after GnRH improved oocyte quality, cleavage rates, blastocyst rates, embryo quality, and the total number of embryos per OPU in lactating Holstein donors. Table 1. Oocyte recovery, cleavage rate, and embryo production results Treatment OPU Oocytes per donor Grade 1 and 2 oocytes Cleavage rate (%) Blastocyst rate (%) Total embryos per OPU Grade 1 embryos per OPU No FSH 22 18.2 30a 69a 22a 3.4a 1.6a 1×FSH 22 17.5 34b 77b 34b 5.3b 3.3b a,bValues with different superscripts in the same column differ (at least P&lt;0.05).
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Barros, F. F. P. C., P. P. M. Teixeira, R. A. R. Uscategui, et al. "Laparoscopic ovum pick-up in spotted paca ( Cuniculus pacas )." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 68, no. 4 (2016): 858–64. http://dx.doi.org/10.1590/1678-4162-8756.

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ABSTRACT The aim of this work is study the laparoscopic ovum pick-up (LapOPU) technique in spotted paca, describing surgery details, complications and oocyte recovery rate. Nine healthy adult non-pregnant captive females were used, in a total of 39 procedures. When the surgical plane of anaesthesia was achieved, the females were positioned at 20º Trendelenburg. Three 6mm trocars were placed on right and left inguinal and hypogastric regions. Abdomen was inflated with CO2 and the intra-abdominal pressure was stablished in 10mmHg. Follicular punctures were performed moving the ovaries with atraumatic forceps. For punctures, an 18-gauge 3.5 inch long needle attached to a vacuum system with pressure not exceeding 65mmHg was used. Oocytes were recovered into 50mL centrifuge tubes with media composed of PBS supplemented with 10 IU/mL of heparin and kept at 36°C. R Software was used for statistical analysis. Data normality distribution (Shapiro test) and variances homoscedasticity (Bartlett test) were tested and descriptive statistics (mean±SD) was used to present the results. It was only possible to perform LapOPU in 30 of 39 laparoscopies (76.92%). The surgical total time was 37.34 ± 18.53 minutes. The total number of visualized follicles, aspirated follicles, and retrieved oocytes were 502, 415, and 155, respectively. And the same parameters per animal were: 14.34 ± 12.23, 11.86 ± 10.03, and 4.43 ± 4.69 respectively. Oocyte recovery rate was 32.56 ± 27.32%. In conclusion, caudal positioning of portals with slight triangulation allows good viewing of the abdominal cavity and eases the manipulation of the ovaries. Thus this described LapOPU technique is feasible in spotted paca and easy to perform.
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Dissertations / Theses on the topic "Oocyte. Ovum"

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Pfender, Sybille Helen. "Studies of asymmetric oocyte division and new genes controlling oocyte maturation." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648232.

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Alton, Michelle. "Control of the oocyte population in mouse ovaries." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81585.

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Oocyte loss and meiotic prophase progression was studied in XY sex-reversed and XO female mice, two mouse models that lack pairing between their sex chromosomes. An arrest at the pachytene stage of meiosis was not observed, nor was a significant loss of oocytes at this stage compared to normal XX control mice. Thus, it was concluded that a pairing checkpoint either does not exist in oocytes or is not as stringent as the one observed in males.
The effect of mutating the pro-apoptotic Bax molecule was studied at three distinct ages corresponding to the time when female germ cells are premeiotic, in meiotic prophase, and arrested in dictyotene. Although it appeared that more germ cells were retained in the Bax homozygous mutant compared to the wild-type and heterozygous mice at 18.5 dpc, by 24.5 dpc all of the mice possessed similar numbers of germ cells. These results indicate a role for Bax in germ cell death but also support the idea that an alternative pathway can compensate for the elimination of this molecule.
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Raposo, Alexandre Augusto da Silva Figueiredo. "Nuclear migration and the regulation of microtubules in the Drosophila oocyte." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611219.

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Ying, Ying. "Male accessory sex glands and oocyte activation at fertilization in the golden hamster /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B20792712.

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Nieuwburg, Ross Willem. "Analysis of the role of dynactin in the polarisation of the cytoskeleton of the Drosophila oocyte." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610258.

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Ying, Ying, and 應嬴. "Male accessory sex glands and oocyte activation at fertilization in the golden hamster." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31239663.

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Fazio, Cynthia Marie. "The influence of meiotic onset on and the role of apoptosis in oocyte death during the meiotic prophase /." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97951.

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Loss of germ cells that entered meiosis at different developmental stages was compared. Mice were injected with BrdU at 13.3, 14.3 or 15.3 days post coitum (dpc) and sacrificed either 3 days after BrdU injection or 4 days post partum (dpp). BrdU-labeled germ cells were detected in ovarian sections through double immunofluorescent staining for BrdU and either GCNA-1 or MVH as a germ cell marker. The results show that the loss of germ cells that entered meiosis at 13.3 or 15.3 dpc was excessive compared to the loss of total germ cells. Such preferential elimination was not found for germ cells that entered meiosis at 14.3 dpc. We conclude that oocyte loss during meiotic prophase is influenced by the timing of meiotic onset.
The mechanism of germ cell loss during ovarian development was tested by the presence of markers for apoptosis. Mouse ovaries were isolated at 12.5 dpc, 18.5 dpc and 2 dpp and cultured with doxorubicin (DXR) to induce cell death. Ovarian histological sections were double immunofluorescent stained for GCNA-1 and cleaved caspase-3 or PARP-1. The results suggest that caspase-3 is not activated in germ cells throughout ovarian development whereas PARP-1 is activated in germ cells at 12.5 dpc and 2 dpp but not at 18.5 dpc. Thus, no evidence has yet been provided to support the hypothesis that oocyte death during the meiotic prophase is mediated by apooptosis.
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Chen, Ying. "The role of steroids in the regulation of oocyte cyst breakdown and primordial follicle assembly in the neonatal mouse ovary." Related electronic resource: Current Research at SU : database of SU dissertations, recent titles available, full text:, 2008. http://wwwlib.umi.com/cr/syr/main.

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Wang, Lei. "Cloning and characterization of a novel oocyte-specific gene Fbos encoding an F-Box protein in rainbow trout (Oncorhynchus mykiss)." Morgantown, W. Va. : [West Virginia University Libraries], 2009. http://hdl.handle.net/10450/10786.

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Thesis (M.S.)--West Virginia University, 2009.
Title from document title page. Document formatted into pages; contains vii, 51 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 46-51).
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Cebrián, Serrano Alberto. "Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem." Doctoral thesis, Universitat Politècnica de València, 2013. http://hdl.handle.net/10251/27646.

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La producción de embriones mediante la recuperación de ovocitos inmaduros por ovum pick up (OPU), y su posterior maduración, fecundación y cultivo en el laboratorio in vitro, presenta numerosos beneficios para optimizar el potencial reproductivo, tanto de hembras como de machos. Además, frente a la superovulación convencional mediante tratamiento hormonal y la recogida de embriones in vivo, la producción in vitro de embriones (PIVE) con ovocitos de OPU ofrece considerables ventajas. Sin embargo, actualmente la PIVE continua siendo ineficiente e incapaz de producir embriones de calidad similar a los in vivo, lo cual ha limitado una aplicación más amplia de esta tecnología. Así pues, el objetivo de esta tesis fue la optimización de la PIVE en ganado vacuno, condicionado por las peculiaridades y deficiencias de la PIVE cuando los ovocitos son recuperados por la técnica de OPU. Con este fin, cinco experimento se llevaron a cabo en esta tesis. En el primero de ellos se estudió el efecto del fluido oviductal bovino (FOb) sobre el desarrollo y la calidad embrionaria (Experimento 1). Las fases del proceso de PIVE en las cuales el cultivo de ovocitos/embriones, bien individualmente o bien en número reducido, pudiera perjudicar el posterior desarrollo hasta el estadio de blastocisto y/o a su calidad, se estudiaron en el Experimento 2. En el Experimento 3 se testó si el desarrollo y la calidad de embriones cultivados in vitro en número reducido podría ser mejorada con la adición conjunta de factor de crecimiento epidérmico, insulina, transferrina y selenio (FCE-ITS) o por el sistema de cultivo de embriones llamado well of well (WOW). Las propiedades protectoras de la melatonina frente a los daños causados por el estrés oxidativo, subsecuentes de las condiciones de PIVE o de un estrés térmico durante la maduración ovocitaria, fueron evaluadas en el Experimento 4. Por último, en el Experimento 5 usamos ovocitos recolectados por OPU para evaluar el efecto del semen sexado sobre
Cebrián Serrano, A. (2013). Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/27646
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Books on the topic "Oocyte. Ovum"

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Patrizia, Ciotti, and Venturoli Stefano, eds. Handbook of human oocyte cryopreservation. Cambridge University Press, 2013.

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Homer, Hayden A. Mammalian oocyte regulation: Methods and protocols. Humana Press, 2013.

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Atlas of the human oocyte and early conceptus. Williams & Wilkins, 1986.

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Borini, Andrea, and Giovanni Coticchio. Preservation of human oocytes. Informa Healthcare, 2009.

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1946-, Elder Kay, and Ebner Thomas, eds. Atlas of oocytes, zygotes and embryos in reproductive medicine. Cambridge University Press, 2012.

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Alan, Trounson, and Wood Carl, eds. Atlas of fine structure of human sperm penetration, eggs, and embryos cultured in vitro. Praeger, 1985.

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Veeck, Lucinda L. Atlas of the humanoocyte and early conceptus. Williams & Wilkins, 1986.

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(Editor), Alan O. Trounson, and Roger G. Gosden (Editor), eds. Biology and Pathology of the Oocyte: Its Role in Fertility and Reproductive Medicine. Cambridge University Press, 2003.

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Alan, Trounson, and Gosden R. G, eds. Biology and pathology of the oocyte: Its role in fertility and reproductive medicine. Cambridge University Press, 2003.

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Gosden, Roger, Alan Trounson, and Ursula Eichenlaub-Ritter. Biology and Pathology of the Oocyte: Role in Fertility, Medicine and Nuclear Reprograming. Cambridge University Press, 2013.

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Book chapters on the topic "Oocyte. Ovum"

1

Bols, Peter E. J., and Tom A. E. Stout. "Transvaginal Ultrasound-Guided Oocyte Retrieval (OPU: Ovum Pick-Up) in Cows and Mares." In Animal Biotechnology 1. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-92327-7_10.

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Carr, Bruce R., and Victor E. Beshay. "Fertilization, Implantation, and Endocrinology of Pregnancy." In Textbook of Endocrine Physiology. Oxford University Press, 2011. http://dx.doi.org/10.1093/oso/9780199744121.003.0013.

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Abstract:
The complex and coordinated set of events leading to sperm and egg maturation and transport in the female genital tract that culminates in fertilization is one of the most remarkable phenomena in nature. This set of events is followed by the equally important unique processes of implantation, fetal maturation, and parturition. The hormonal changes that regulate these events are dependent on the close interaction of the fetal-placental-maternal unit. Just before ovulation, the egg, which has been arrested in the diplotene stage, completes the first meiotic division and forms the first polar body. The second meiotic division starts at the time of ovulation but ends only after fertilization by a sperm. The process of egg maturation is regulated through a closely interrelated set of hormonal events, most notably involving follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estrogen. At the time of ovulation the fimbria of the oviduct are closely applied to the surface of the ovary. The extruded oocyte and adherent granulosa cells, known as the cumulus oophorus, is collected by the ciliated fimbrial end of the fallopian tube. The transport of the egg into the end of the fallopian tube occurs within minutes and is regulated primarily by ciliary action. The cumulus cells are able to communicate with one another via a network of intercellular bridges through the zona pellucida to the perivitelline space. The cumulus cells have also been reported to play a role in nutrition and maintenance of the ovum. There are three different stages of passage of the ovum through the fallopian tube. The first stage includes the transfer of the ovum from the fimbriated end of the fallopian tube until the egg reaches and is retained at the ampullary-isthmic junction. The ampullary-isthmic junction is a functional block but is not a clearly defined anatomical structure. The ovum remains at this junction for 1–2 days, during which time fertilization occurs.
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