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Journal articles on the topic 'Oocyte'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Budna, Joanna, Artur Bryja, Piotr Celichowski, et al. "Genes of cellular components of morphogenesis in porcine oocytes before and after IVM." Reproduction 154, no. 4 (2017): 535–45. http://dx.doi.org/10.1530/rep-17-0367.

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Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating that the gene expression between immature and mature oocytes differs significantly. This study focuses on the genes responsible for the cellular comp

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2

Pedersen, Hanne Skovsgaard, Peter Løvendahl, Knud Larsen, Lone Bruhn Madsen, and Henrik Callesen. "Porcine oocyte mtDNA copy number is high or low depending on the donor." Zygote 24, no. 4 (2015): 617–23. http://dx.doi.org/10.1017/s0967199415000611.

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SummaryOocyte capacity is relevant in understanding decreasing female fertility and in the use of assisted reproductive technologies in human and farm animals. Mitochondria are important to the development of a functionally good oocyte and the oocyte mtDNA copy number has been introduced as a useful parameter for prediction of oocyte competence. The aim of this study was to investigate: (i) if the oocyte donor has an influence on its oocyte's mtDNA copy number; and (ii) the relation between oocyte size and mtDNA copy number using pre- and postpubertal pig oocytes. Cumulus–oocyte complexes were

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Walker, Bailey N., and Fernando H. Biase. "The blueprint of RNA storages relative to oocyte developmental competence in cattle (Bos taurus)." Biology of Reproduction 102, no. 4 (2020): 784–94. http://dx.doi.org/10.1093/biolre/ioaa015.

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Abstract From the time oocytes leave quiescence, there are constant microenvironmental influences contributing to development, thus acquiring developmental competence is not a simple, linear phenomenon. During folliculogenesis, oocytes experience many morphological and cytological changes that contribute toward the acquisition of developmental competence, a process defined by an oocyte’s ability to progress through folliculogenesis, be fertilized, undergo cleavage, and develop into an embryo. Many factors, such as ovarian follicle size, cow age, and the morphology of the cumulus–oocyte complex

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Ritter, Lesley J., Satoshi Sugimura, and Robert B. Gilchrist. "Oocyte Induction of EGF Responsiveness in Somatic Cells Is Associated With the Acquisition of Porcine Oocyte Developmental Competence." Endocrinology 156, no. 6 (2015): 2299–312. http://dx.doi.org/10.1210/en.2014-1884.

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Abstract Oocytes progressively acquire the competence to support embryo development as oogenesis proceeds with ovarian folliculogenesis. The objectives of this study were to investigate oocyte-secreted factor (OSF) participation in the development of somatic cell epidermal growth factor (EGF) responsiveness associated with oocyte developmental competence. A well-established porcine model was employed using oocytes from small (&lt;4 mm) vs medium sized (&gt;4 mm) antral follicles, representing low vs moderate developmental competence, respectively. Cumulus-oocyte complexes (COCs) were t

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Virant-Klun, Irma, Katja Knez, Tomaz Tomazevic, and Thomas Skutella. "Gene Expression Profiling of Human Oocytes Developed and MaturedIn VivoorIn Vitro." BioMed Research International 2013 (2013): 1–20. http://dx.doi.org/10.1155/2013/879489.

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The quality of the human oocyte determines the success of fertilization and affects the consequent embryo development, pregnancy and birth; it therefore serves as a basis for human reproduction and fertility. The possibility to evaluate oocyte quality in thein vitrofertilization programme is very limited. The only criterion which is commonly used to evaluate oocyte quality is its morphology. There is a mass of oocytes in thein vitrofertilization programme which are not fertilized in spite of normal morphology. In the past, several attempts focused on oocyte gene expression profiling by differe

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Němeček, David, Markéta Dvořáková, Ivona Heroutová, Eva Chmelíková, and Markéta Sedmíková. "Anti-apoptotic properties of carbon monoxide in porcine oocyte duringin vitroaging." PeerJ 5 (October 6, 2017): e3876. http://dx.doi.org/10.7717/peerj.3876.

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If fertilization of matured oocyte does not occur, unfertilized oocyte undergoes aging, resulting in a time-dependent reduction of the oocyte’s quality. The aging of porcine oocytes can lead to apoptosis. Carbon monoxide (CO), a signal molecule produced by the heme oxygenase (HO), possesses cytoprotective and anti-apoptotic effects that have been described in somatic cells. However, the effects of CO in oocytes have yet to be investigated. By immunocytochemistry method we detected that both isoforms of heme oxygenase (HO-1 and HO-2) are present in the porcine oocytes. Based on the morphologica

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Liu, Rui-Hua, Yong-Hai Li, Li-Hong Jiao, Xiao-Ning Wang, Hong Wang, and Wei-Hua Wang. "Extracellular and intracellular factors affecting nuclear and cytoplasmic maturation of porcine oocytes collected from different sizes of follicles." Zygote 10, no. 3 (2002): 253–60. http://dx.doi.org/10.1017/s0967199402002332.

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Nuclear and cytoplasmic maturation of porcine oocytes collected from different sizes of follicles were examined. Oocyte-cumulus complexes were collected from small (1-2 mm in diameter), medium (3-6 in diameter) and large (7-8 mm in diameter) follicles and cultured in a modified tissue culture medium 199 for 44 h. Nuclear maturation was evaluated after orcein staining, and cytoplasmic maturation was evaluated by intracellular glutathione (GSH) assay. Oocyte diameter, cumulus morphology, steroid hormones and glutathione in the follicular fluid (FF), were also examined. Significantly higher propo

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Zuccotti, Maurizio, Anna Piccinelli, Nicola Marziliano, Silvia Mascheretti, and Carlo Alberto Redi. "Development and loss of the ability of mouse oolemma to fuse with spermatozoa." Zygote 2, no. 4 (1994): 333–39. http://dx.doi.org/10.1017/s096719940000215x.

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SummaryTo further our Knowledge on the mechanisms and molecules involved in mouse sperm–oocyte plasma membrane interaciton, exteraction, experiments were carried out to determine the stage during oogenesis at which an oocte acquires the capacity ot fuse with acrosome-reacted sperm. Zona-ferr oocytes 10 μm in diametes do not fuse with sperm. Oolemma fusibility is first acquired when the oocyte reaches about 20μm in diameter. Fusibility is maniatained even after fertilisation has accurred and is lost completely by the 4–cell stage.

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9

Zhai, Rongan, Miao Hao, Yong Wang, Changhai Ru, and Junhui Zhu. "Robotic Manipulation of Cumulus–Oocyte Complexes for Cumulus Cell Removal." Applied Sciences 14, no. 18 (2024): 8450. http://dx.doi.org/10.3390/app14188450.

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The removal of cumulus cells from cumulus–oocyte complexes is a critical step in clinical in vitro fertilization. Since the oocyte is partially occluded by the surrounding cumulus cells and individual cumulus cells are small in size, it is difficult for embryologists to assess the oocyte's maturity before cumulus cell removal and to completely remove all the cumulus cells manually . Furthermore, it is easy for the oocyte to become lost inside the micropipette during aspiration due to the inaccuracy of manual control. To deal with these difficulties, a robotic system was developed to completely

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Talreja, Deepa, Chirag Gupta, Hrishikesh Pai, and Nandita Palshetkar. "Oocyte Vitrification: A Comparative Analysis Between Fresh and Cryopreserved Oocytes in an Oocyte Donation Program." Fertility & Reproduction 02, no. 01 (2020): 9–13. http://dx.doi.org/10.1142/s2661318220500024.

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Background: Oocyte Cryopreservation has become an important part of infertility treatment for various reasons such as fertility preservation in women going for oncological treatment; in oocyte donation cycles; in eliminating several religious, ethical, and legal concerns of embryo freezing and in women who wish to delay childbirth. The newer ”vitrification” technique for freezing has further improved the success rates for actual conception than the earlier method of slow freezing. A successful oocyte freezing program can help in establishment of oocyte banks, which would help to provide compat

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Gong, Zhaoqing, Yujie Wang, Jiayi Tang, et al. "Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo." PLOS ONE 20, no. 2 (2025): e0312241. https://doi.org/10.1371/journal.pone.0312241.

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Purpose Embryo engineering requires a large number of oocytes, which undergo in vitro maturation (IVM). Understanding how to select the best quality oocytes is key to improving IVM efficiency. Oocytes have different germinal vesicle (GV) chromatin configurations, which may explain the heterogeneity in oocyte quality during IVM. However, no reports have categorized, the chromatin configuration of rat GVs or evaluated, the association between the chromatin configuration and oocytes development. Methods The GV chromatin configuration of rat oocytes was divided into seven types according to the de

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Uh, Kyungjun, Alayna Hay, Paula Chen, Emily Reese, and Kiho Lee. "Design of novel oocyte activation methods: the role of zinc." Biology of Reproduction 106, no. 2 (2021): 264–73. http://dx.doi.org/10.1093/biolre/ioab235.

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Abstract Oocyte activation occurs at the time of fertilization and is a series of cellular events initiated by intracellular Ca2+ increases. Consequently, oocytes are alleviated from their arrested state in meiotic metaphase II (MII), allowing for the completion of meiosis. Oocyte activation is also an essential step for somatic cell nuclear transfer and an important tool to overcome clinical infertility. Traditional artificial activation methods aim to mimic the intracellular Ca2+ changes which occur during fertilization. Recent studies emphasize the importance of cytoplasmic Zn2+ on oocyte m

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Tharasanit, T., S. Colleoni, G. Lazzari, B. Colenbrander, C. Galli, and T. A. E. Stout. "Effect of cumulus morphology and maturation stage on the cryopreservability of equine oocytes." Reproduction 132, no. 5 (2006): 759–69. http://dx.doi.org/10.1530/rep.1.01156.

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Oocyte cryopreservation is a potentially valuable way of preserving the female germ line. However, the developmental competence of cryopreserved oocytes is presently poor. This study investigated whether the morphology of the cumulus complex surrounding an immature equine oocyte and/or the oocyte’s stage of maturation affect its cryopreservability. Compact (Cp) and expanded (Ex) cumulus oocyte complexes (COCs) were vitrified either shortly after recovery (germinal vesicle stage, GV) or after maturation in vitro (IVM); cryoprotectant-treated and -untreated non-frozen oocytes served as controls.

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Bhatia, Priyankaa, Judith Tafur, Ruchi Amin, et al. "Condensate-forming eIF4ET ensures adequate levels of meiotic proteins to support oocyte storage." Life Science Alliance 8, no. 8 (2025): e202503387. https://doi.org/10.26508/lsa.202503387.

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Animals store oocytes in a dormant state for weeks to decades before ovulation. The homeostatic programs that oocytes use to endure long-term storage are poorly understood. Using female nematodes as a short-lived model, we found that oocyte formation and storage required IFET-1, the conserved eIF4E-transporter protein (eIF4ET). IFET-1 co-assembled with CAR-1 (Lsm14) to form micron-scale condensates in stored oocytes, which dissipated after oocyte activation. Depletion of IFET-1 destabilized the stored oocyte proteome, leading to lower translation, a decline in microtubule maintenance proteins,

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Curnow, E. C., J. P. Ryan, D. M. Saunders, and E. S. Hayes. "Developmental potential of bovine oocytes following IVM in the presence of glutathione ethyl ester." Reproduction, Fertility and Development 22, no. 4 (2010): 597. http://dx.doi.org/10.1071/rd09228.

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Glutathione (GSH) is synthesised during oocyte maturation and represents the oocyte’s main non-enzymatic defence against oxidative stress. Inadequate defence against oxidative stress may be related to poor embryo quality and viability. In the present study, bovine oocytes were matured in vitro in the presence of GSH ethyl ester (GSH-OEt), a cell permeable GSH donor, and its effects on subsequent fertilisation and embryo development were assessed. GSH-OEt significantly increased the GSH content of IVM oocytes without affecting fertilisation or Day 3 cleavage rates. Maturation in the presence of

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Pedersen, H. S., Y. Liu, R. Li, et al. "Selection of pre- versus postpubertal pig oocytes for parthenogenetic activation and somatic cell nuclear transfer." Reproduction, Fertility and Development 27, no. 3 (2015): 544. http://dx.doi.org/10.1071/rd13283.

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Pig oocytes have been used increasingly for in vitro production techniques in recent years. The slaughterhouse-derived oocytes that are often used are mostly of prepubertal origin. The aims of the present study were to compare the developmental competence between pre- and postpubertal pig oocytes, and to develop a simple and practical method for the selection of prepubertal pig oocytes for parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) based on oocyte morphology after IVM and oocyte inside zona pellucida (ZP) diameter (‘small’ ≤110 µm; ‘medium’ >110 µm; ‘large’ ≥12

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Chen, Ying, Wendy N. Jefferson, Retha R. Newbold, Elizabeth Padilla-Banks, and Melissa E. Pepling. "Estradiol, Progesterone, and Genistein Inhibit Oocyte Nest Breakdown and Primordial Follicle Assembly in the Neonatal Mouse Ovary in Vitro and in Vivo." Endocrinology 148, no. 8 (2007): 3580–90. http://dx.doi.org/10.1210/en.2007-0088.

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In developing mouse ovaries, oocytes develop as clusters of cells called nests or germ cell cysts. Shortly after birth, oocyte nests dissociate and granulosa cells surround individual oocytes forming primordial follicles. At the same time, two thirds of the oocytes die by apoptosis, but the link between oocyte nest breakdown and oocyte death is unclear. Although mechanisms controlling breakdown of nests into individual oocytes and selection of oocytes for survival are currently unknown, steroid hormones may play a role. Treatment of neonatal mice with natural or synthetic estrogens results in

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Safaefar, Firooz, Javad Karamdel, Hadi Veladi, and Masoud Maleki. "Design and implementation of a lab-on-a-chip for assisted reproductive technologies." BioImpacts 14, no. 4 (2023): 28902. http://dx.doi.org/10.34172/bi.2023.28902.

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Introduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study, a novel microfluidic chip was designed and manufactured using conventional soft lithography methods. Methods: This research proposes the utilization of a microfluidic chip as a substitute for the conventional manual procedures involved in oocyte denudation, trapping, and immobilization. The microfl

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Fernandes, C. A. F., P. G. V. Oliveira, C. H. B. Oliveira, F. H. V. Hazin, and P. Travassos. "Oocyte development and fecundity type of the Brazilian Snapper Lutjanus alexandrei Moura & Lindeman, 2007 (Perciformes: Lutjanidae)." Brazilian Journal of Biology 76, no. 1 (2016): 126–35. http://dx.doi.org/10.1590/1519-6984.14714.

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Abstract Lutjanid species exhibit multiple spawning behaviour during an extended spawning season in warm months, asynchronous oocyte development and indeterminate fecundity. Although early studies have contributed to knowledge of the reproductive cycle of many species within the group, they have not considered aspects about the number of cortical alveoli oocyte stage throughout maturity phases along spawning season. The latter aspect is also considered very important to confirm indeterminate fecundity hypothesis. In the present study, were analyzed 154 Brazilian snapper Lutjanus alexandrei fem

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Maside, Carolina, Irene Sánchez-Ajofrín, Daniela Medina-Chávez, Benner Alves, José Julián Garde, and Ana Josefa Soler. "Oocyte Morphometric Assessment and Gene Expression Profiling of Oocytes and Cumulus Cells as Biomarkers of Oocyte Competence in Sheep." Animals 11, no. 10 (2021): 2818. http://dx.doi.org/10.3390/ani11102818.

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Oocyte quality is crucial for subsequent embryo development and so it is a major challenge in assisted reproductive technologies. The aim of the present work was to evaluate the morphometric parameters of oocytes (experiment 1) and the relative gene expression of oocytes and cumulus cells (CCs) (experiment 2) as biomarkers of oocyte quality after individually culturing them (one oocyte or embryo/drop). In experiment 1, individually matured oocytes were measured and classified into small, intermediate, and large oocytes after a cluster analysis, based on total diameter (with zona pellucida, ZP)

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Yamazaki, Yukiko, Teruhiko Wakayama, and Ryuzo Yanagimachi. "Contribution of cumulus cells and serum to the maturation of oocyte cytoplasm as revealed by intracytoplasmic sperm injection (ICSI)." Zygote 9, no. 4 (2001): 277–82. http://dx.doi.org/10.1017/s0967199401001307.

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The fertilisability and developmental capacity of mouse oocytes matured in vitro were examined by in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI). While more than 50% of cumulus-enclosed oocytes were fertilised by IVF after maturation in serum-supplemented medium, none were fertilised when the oocytes matured without serum. By ICSI, the majority (78-94%) of the oocytes were fertilised regardless of the presence or absence of serum in oocyte maturation media. Although the majority (88-92%) of cumulus-free germinal vesicle oocytes underwent nuclear maturation in both ser

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Uozumi, T., and H. Funahashi. "272 INTRACELLULAR NITRIC OXIDE LEVEL OF PORCINE OOCYTES IS NEGATIVELY CORRELATED WITH OOCYTE MATURATION RATE AND CUMULUS EXPANSION INDEX IN A CHEMICALLY DEFINED MEDIUM." Reproduction, Fertility and Development 23, no. 1 (2011): 234. http://dx.doi.org/10.1071/rdv23n1ab272.

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Nitric oxide (NO) has been known to inhibit nuclear maturation in cumulus–enclosed oocytes in rodents. The objective of this study was to examine if meiotic stimulators, such as dibutyryl cAMP and epidermal growth factor (EGF), influence intracellular NO level of oocytes and if the level is correlated with oocyte maturation rate and cumulus expansion in a chemically defined medium. Oocyte–cumulus complexes (OCC) were aspirated from mid-size follicles (3–6 mm in diameter) of prepuberal porcine ovaries. The OCC were cultured in modified porcine oocyte medium with various supplements – gonadotrop

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Chen, Jing, Maggie M. Chi, Kelle H. Moley, and Stephen M. Downs. "cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase." REPRODUCTION 138, no. 5 (2009): 759–70. http://dx.doi.org/10.1530/rep-08-0535.

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cAMP plays a critical role in the control of oocyte maturation, as a high level of cAMP maintains oocyte arrest at the first meiotic prophase. Yet this study shows that pulsing meiotically arrested denuded oocytes (DO) with cAMP induces oocyte maturation through the activation of AMP-activated protein kinase (PRKA). Short-term (3 h) pulsing of meiotically arrested oocytes with forskolin, an adenyl cyclase (AC) activator, increased oocyte cAMP, led to elevated AMP, and induced oocyte meiotic resumption compared to oocytes continuously cultured in the control medium with or without forskolin. We

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Lisle, R. S., K. Anthony, M. A. Randall, and F. J. Diaz. "Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes." REPRODUCTION 145, no. 4 (2013): 381–90. http://dx.doi.org/10.1530/rep-12-0338.

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Zinc increases in the oocyte during maturation and is required for progression and completion of meiosis. The objective of this study was to determine whether cumulus cells regulate the levels of free intracellular zinc in the oocyte during maturation. In the cumulus–oocyte complex (COC) the relative level of free intracellular zinc was almost fourfold higher in cumulus cells compared with the resident germinal vesicle-stage oocyte. Removal of cumulus cells caused a fourfold increase in intracellular zinc in the oocyte by 1 h after cumulus cell removal, but subsequent coculture of denuded oocy

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Wang, Qiang, Maggie M. Chi, Tim Schedl, and Kelle H. Moley. "An intercellular pathway for glucose transport into mouse oocytes." American Journal of Physiology-Endocrinology and Metabolism 302, no. 12 (2012): E1511—E1518. http://dx.doi.org/10.1152/ajpendo.00016.2012.

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Glucose is an essential nutrient for mammalian cells. Emerging evidence suggests that glucose within the oocyte regulates meiotic maturation. However, it remains controversial as to whether, and if so how, glucose enters oocytes within cumulus-oocyte complexes (COCs). We used a fluorescent glucose derivative (6-NBDG) to trace glucose transport within live mouse COCs and employed inhibitors of glucose transporters (GLUTs) and gap junction proteins to examine their distinct roles in glucose uptake by cumulus cells and the oocyte. We showed that fluorescent glucose enters both cumulus-enclosed an

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Abe, H., H. Shiku, S. Aoyagi, T. Matsue, and H. Hoshi. "319 OXYGEN CONSUMPTION OF BOVINE CUMULUS CELLS AND OOCYTES CULTURED IN DIFFERENT CULTURE SYSTEMS FOR OOCYTE MATURATION." Reproduction, Fertility and Development 18, no. 2 (2006): 267. http://dx.doi.org/10.1071/rdv18n2ab319.

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Oxygen consumption is a ubiquitous parameter that can provide valuable information on metabolic mechanisms and on oocyte and embryo quality. Recently, we succeeded in non-invasively and quantitatively determining oxygen consumption of individual bovine embryos by scanning electrochemical microscopy (SECM). The aim of this study was to assess by SECM the oxygen consumption of bovine cumulus cells and oocytes cultured in serum-free and serum-supplemented media for oocyte maturation. Bovine cumulus–oocyte complexes (COCs) were obtained from ovarian follicles 2–6 mm in diameter. COCs were cultured

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Guo, Jing, Teng Zhang, Yueshuai Guo, et al. "Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice." Proceedings of the National Academy of Sciences 115, no. 23 (2018): E5326—E5333. http://dx.doi.org/10.1073/pnas.1800352115.

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MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. Here we show that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. cKO of Mtor in nongrowing primordial oocytes caused defective follicular development leading to progressive degeneration of oocytes and loss of granulosa cell identity coincident with the acquisition of immature Sertoli cell-like cha

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Curnow, E. C., J. Ryan, D. Saunders, and E. S. Hayes. "241 STRATEGIES TO IMPROVE GLUTATHIONE CONTENT OF IN VITRO-MATURED BOVINE OOCYTES." Reproduction, Fertility and Development 20, no. 1 (2008): 200. http://dx.doi.org/10.1071/rdv20n1ab241.

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Glutathione is the main non-enzymatic defense against oxidative stress and a critical part of oocyte maturation and normal fertilization. Our aim was to test different strategies to manipulate cellular glutathione (GSH) content of bovine in-vitro-matured (IVM) oocytes and study the development of embryos produced from such oocytes. The reducing agents lipoic acid (LA, intracellular) and dihydrolipoic acid (DHLA, extracellular) were compared to the cell-permeable reduced glutathione (GSH) donor glutathione ethyl ester (OET) for their effect on oocyte GSH content, oocyte maturation, and blastocy

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Ali, Abbas Musa, and Saad Akram Hatif. "Low oocyte quality related with the aging ewes." Iraqi Journal of Veterinary Medicine 37, no. 2 (2013): 261–65. http://dx.doi.org/10.30539/ijvm.v37i2.1391.

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This study was conducted to know the effect of ewe age on oocyte quality as well as the relationsbetween oocyte viability and normal uterine condition. Eighty three (83) reproductive systems ofnon-pregnant ewes were collected from Al-shulla abattoir. The Total oocytes were aspirated from right ovaries reached 61.45% and 38.55% from left ovaries. Immediately after aspiration, the oocytes were examined by light microscopic and conceded as mature if surrounded completely with cumulus oopherus. While the stained oocytes by trypan blue were conceded as dead oocytes and excluded. According to ewes a

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Yagüe-Serrano, Roberto, Andrea Palomar, Alicia Quiñonero, et al. "Oocyte Competence, Embryological Outcomes and miRNA Signature of Different Sized Follicles from Poor Responder Patients." International Journal of Molecular Sciences 25, no. 19 (2024): 10237. http://dx.doi.org/10.3390/ijms251910237.

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Poor ovarian response (POR) patients often face the risk of not having enough competent oocytes. Then, aspirating small follicles could serve as a strategy to increase their number. Many efforts have been addressed to associate follicular size with oocyte competence, but results are controversial. Therefore, our study aimed to evaluate oocyte maturation and developmental competence, along with a non-invasive oocyte-maturation-related miRNA signature in oocytes retrieved from both large and small follicles. A total of 178 follicles, from 31 POR patients, were aspirated and measured on the day o

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Lu, Yujie, Yue Zhang, Jia-Qian Liu, et al. "Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis." PeerJ 6 (June 20, 2018): e5111. http://dx.doi.org/10.7717/peerj.5111.

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Background Aflatoxin B1 (AFB1), deoxynivalenol (DON), HT-2, ochratoxin A (OTA), zearalenone (ZEA) are the most common mycotoxins that are found in corn-based animal feed which have multiple toxic effects on animals and humans. Previous studies reported that these mycotoxins impaired mammalian oocyte quality. However, the effective concentrations of mycotoxins to animal oocytes were different. Methods In this study we aimed to compare the sensitivity of mouse and porcine oocytes to AFB1, DON, HT-2, OTA, and ZEA for mycotoxin research. We adopted the polar body extrusion rate of mouse and porcin

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Jiang, Yao, Yingting He, Xiangchun Pan, Penghao Wang, Xiaolong Yuan, and Bin Ma. "Advances in Oocyte Maturation In Vivo and In Vitro in Mammals." International Journal of Molecular Sciences 24, no. 10 (2023): 9059. http://dx.doi.org/10.3390/ijms24109059.

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The quality and maturation of an oocyte not only play decisive roles in fertilization and embryo success, but also have long-term impacts on the later growth and development of the fetus. Female fertility declines with age, reflecting a decline in oocyte quantity. However, the meiosis of oocytes involves a complex and orderly regulatory process whose mechanisms have not yet been fully elucidated. This review therefore mainly focuses on the regulation mechanism of oocyte maturation, including folliculogenesis, oogenesis, and the interactions between granulosa cells and oocytes, plus in vitro te

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Li, Yong-Hai, Yi Hou, Wei Ma, et al. "Localization of CD9 in pig oocytes and its effects on sperm–egg interaction." Reproduction 127, no. 2 (2004): 151–57. http://dx.doi.org/10.1530/rep.1.00006.

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CD9 is a cell surface protein that participates in many cellular processes, such as cell adhesion. Fertilization involves sperm and oocyte interactions including sperm binding to oocytes and sperm–oocyte fusion. Thus CD9 may play an essential role during fertilization in mammals. The present study was conducted to examine whether CD9 is present in porcine gametes and whether it participates in the regulation of sperm–oocyte interactions. The presence of CD9 in ovarian tissues, oocytes and spermatozoa was examined by immunohistochemistry, immunofluorescence and immunoblotting. Sperm binding and

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Hipp, H. S., A. J. Gaskins, Z. P. Nagy, S. M. Capelouto, D. B. Shapiro, and J. B. Spencer. "Effect of oocyte donor stimulation on recipient outcomes: data from a US national donor oocyte bank." Human Reproduction 35, no. 4 (2020): 847–58. http://dx.doi.org/10.1093/humrep/deaa003.

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Abstract STUDY QUESTION How does ovarian stimulation in an oocyte donor affect the IVF cycle and obstetric outcomes in recipients? SUMMARY ANSWER Higher donor oocyte yields may affect the proportion of usable embryos but do not affect live birth delivery rate or obstetric outcomes in oocyte recipients. WHAT IS KNOWN ALREADY In autologous oocyte fresh IVF cycles, the highest live birth delivery rates occur when ~15–25 oocytes are retrieved, with a decline thereafter, perhaps due to the hormone milieu, with super-physiologic estrogen levels. There are scant data in donor oocyte cycles, wherein t

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Mohd Faizal, Ahmad, Yodo Sugishita, Yuki Suzuki-Takahashi, et al. "Twenty-first century oocyte cryopreservation—in vitro maturation of immature oocytes from ovarian tissue cryopreservation in cancer patients: A systematic review." Women's Health 18 (January 2022): 174550572211142. http://dx.doi.org/10.1177/17455057221114269.

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Objectives: Our review aimed to consolidate the latest update on the application of in vitro maturation among immature oocyte harvest in combination with ovarian tissue cryopreservation known as ovarian tissue oocyte–in vitro maturation. Methods: A thorough search for relevant studies was conducted via PubMed, Google Scholar, EMBASE, and clinical.gov databases up to December 2020. The primary outcome was the oocyte maturation rate, which measured the number of immature oocytes (geminal vesicle stage) that progressed to mature oocytes (meiosis II stage) following in vitro maturation. The second

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Finn, Roderick Nigel, Gunn C. Østby, Birgitta Norberg, and Hans Jørgen Fyhn. "In vivo oocyte hydration in Atlantic halibut (Hippoglossus hippoglossus); proteolytic liberation of free amino acids, and ion transport, are driving forces for osmotic water influx." Journal of Experimental Biology 205, no. 2 (2002): 211–24. http://dx.doi.org/10.1242/jeb.205.2.211.

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SUMMARY The in vivo swelling and hydration of maturing oocytes of Atlantic halibut Hippoglossus hippoglossus were studied in order to characterise the osmotic mechanism underlying oocyte hydration in oviparous marine teleosts that spawn pelagic eggs. Sequential biopsies from two females, spanning four hydration cycles, were examined by osmometry, solute analysis and electrophoresis of dissected hydrating oocytes and ovulated eggs. The hydration cycle of the biopsied halibuts lasted 33–54 h. The majority of ovarian oocytes existed in a pre-hydrated condition (individual wet mass approx. 3.7 mg,

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Rodriguez, Karina F., and Charlotte E. Farin. "Gene transcription and regulation of oocyte maturation." Reproduction, Fertility and Development 16, no. 2 (2004): 55. http://dx.doi.org/10.1071/rd03078.

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The developmental potential of an embryo is dependent on the developmental potential of the oocyte from which it originates. The process of oocyte maturation is critical for the efficient application of biotechnologies such as in vitro embryo production and mammalian cloning. However, the overall efficiency of in vitro maturation remains low because oocytes matured in vitro have a lower developmental competence than oocytes matured in vivo. Furthermore, oocytes that have been exposed to gonadotropins have greater developmental competence than oocytes matured in the absence of gonadotropins. By

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Piekarski, Nadine, Theodore R. Hobbs, Darla Jacob, et al. "A Comparison of Oocyte Yield between Ultrasound-Guided and Laparoscopic Oocyte Retrieval in Rhesus Macaques." Animals 13, no. 19 (2023): 3017. http://dx.doi.org/10.3390/ani13193017.

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Obtaining quality oocytes is a prerequisite for ART-based studies. Here we describe a method for transabdominal ultrasound-guided (US) oocyte retrieval in rhesus macaques (Macaca mullata) and compare it to the standard surgical approach using laparoscopy (LAP). We analyzed oocyte yield from six continuous reproductive seasons (2017–2023) that included n = 177 US-guided and n = 136 laparoscopic oocyte retrievals. While the ultrasound-guided technique retrieved significantly fewer oocytes on average (LAP: 40 ± 2 vs. US: 27 ± 1), there was no difference in the number of mature metaphase II oocyte

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Astbury, Pia, Goutham N. Subramanian, Jessica Greaney, Chris Roling, Jacqui Irving, and Hayden A. Homer. "The Presence of Immature GV− Stage Oocytes during IVF/ICSI Is a Marker of Poor Oocyte Quality: A Pilot Study." Medical Sciences 8, no. 1 (2020): 4. http://dx.doi.org/10.3390/medsci8010004.

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Here we investigate whether the presence of germinal vesicle-stage oocytes (GV− oocytes) reflects poor oocyte developmental competence (or quality). This was a prospective, non-randomised, cohort pilot-study involving 60 patients undergoing in vitro fertilization/ intracytoplasmic sperm injection for whom complete pregnancy outcome data were available. Patients in whom GV− oocytes were retrieved (GV+) at transvaginal oocyte retrieval (TVOR) were compared with those from whom no GVs were retrieved (GV−). We found that GV+ (n = 29) and GV− (n = 31) patients were similarly aged (35.4 vs. 36.4 yea

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Petersen, Morten R., Michael Hansen, Birthe Avery, and Ingrid B. Bøgh. "A Method for Chronological Intravital Imaging of Bovine Oocytes during In Vitro Maturation." Microscopy and Microanalysis 14, no. 6 (2008): 549–60. http://dx.doi.org/10.1017/s1431927608080872.

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AbstractOocyte maturation is known to affect the chances for successful fertilization, embryonic development, establishment of pregnancy and delivery of a live, healthy, and viable offspring. Two-photon laser scanning microscopy (TPLSM) has previously been used to evaluate early embryonic development without a detectable impairment of subsequent development, but has never been applied to assess mammalian oocytes throughout in vitro maturation (IVM). Visualization of structures within live oocytes during IVM, followed by fertilization and embryo culture, may improve the understanding of oocyte

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Andreu-Vázquez, C., F. López-Gatius, I. García-Ispierto, M. J. Maya-Soriano, R. H. F. Hunter, and M. López-Béjar. "Does heat stress provoke the loss of a continuous layer of cortical granules beneath the plasma membrane during oocyte maturation?" Zygote 18, no. 4 (2010): 293–99. http://dx.doi.org/10.1017/s0967199410000043.

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SummaryThe objective of the present study was to evaluate the influence of heat stress on bovine oocyte maturation. Both nuclear stage and distribution of cortical granules (CG) were simultaneously evaluated in each oocyte. Oocyte overmaturation under standard conditions of culture was also evaluated. For this purpose, logistic regression procedures were used to evaluate possible effects of factors such as heat stress, overmaturation, replicate, CG distribution and metaphase II (MII) morphology on oocyte maturation. Based on the odds ratio, oocytes on heat stressed (HSO) and overmaturated (OMO

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Oliveira, Reginaldo Luis, Katia Cristina Silva-Santos, Suellen Miguez Gonzalez, et al. "Proliferative activity of oocytes in multi-oocyte follicles of bovine ovary." Semina: Ciências Agrárias 38, no. 6 (2017): 3591. http://dx.doi.org/10.5433/1679-0359.2017v38n6p3591.

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We characterized the proliferative activity of multi-oocyte follicles with anti-nuclear antigen of proliferating cells (PCNA). Ovaries (n = 12) from heifers were processed for histology. From 789 multi-oocytes follicles observed, only 11 were considered appropriated for immunostaining, since they presented all nuclei of the oocytes clearly visible. All multi-oocyte follicles were positive for PCNA, but some oocytes showed no proliferative activity. We conclude that oocytes in multi-oocyte follicles seem to be in different stages of the cell cycle.

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Tanaka, M., J. D. Hennebold, J. Macfarlane, and E. Y. Adashi. "A mammalian oocyte-specific linker histone gene H1oo: homology with the genes for the oocyte-specific cleavage stage histone (cs-H1) of sea urchin and the B4/H1M histone of the frog." Development 128, no. 5 (2001): 655–64. http://dx.doi.org/10.1242/dev.128.5.655.

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Oocytes and early embryos of multiple (non-mammalian) species lack the somatic form of the linker histone H1. To the best of our knowledge, a mammalian oocyte-specific linker (H1) histone(s) has not, as yet, been reported. We have uncovered the cDNA in question in the course of a differential screening (suppression subtractive hybridization (SSH)) project. Elucidation of the full-length sequence of this novel 1.2 kb cDNA led to the identification of a 912 bp open reading frame. The latter encoded a novel 34 kDa linker histone protein comprised of 304 amino acids, tentatively named H1oo. Amino

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Servou, Eleni, Eudoxia Schismenou, and Stylianos Somarakis. "Quantitative Analysis of Ovarian Dynamics of European Sardine Sardina pilchardus (Walbaum, 1792) during Its Spawning Period." Fishes 8, no. 5 (2023): 226. http://dx.doi.org/10.3390/fishes8050226.

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Fish with indeterminate fecundity spawn multiple times throughout a protracted reproductive period. During that period several ovulation events succeed one another, and different oocyte developmental stages co-occur in the ovaries with new oocytes consistently recruiting from one growth phase to the next to form the sequential batches. In this study, we examined in detail the oocyte recruitment and development pattern of the sequential batches in a commercially important fish with indeterminate fecundity, the European sardine. The numbers and sizes of oocytes at different developmental stages

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Абакушина, Е. В., Ю. В. Гельм та А. С. Миценык. "Флуоресцентный микроскопический анализ жизнеспособности ооцитов млекопитающих после витрификации-=SUP=-*-=/SUP=-". Журнал технической физики 126, № 5 (2019): 611. http://dx.doi.org/10.21883/os.2019.05.47660.9-19.

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AbstractThe paper reports the results of a fluorescent microscopy analysis of the viability of oocytes from cattle and pigs after vitrification. Oocytes were frozen in a vitrification media containing varying concentrations of cryoprotectors in several steps with subsequent vitrification. After cryobank storage for 14 days, experimental samples were thawed and oocyte viability was analyzed by oocyte morphology assessment and fluorescent microscopy. Two different kits were used to stain oocytes, one specific for necrosis/apoptosis (Propidium iodide/Alexa Fluor 488 Annexin) and the other specifi

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Herrick, Jason R. "Reversible meiotic arrest in feline oocytes." Reproduction, Fertility and Development 26, no. 2 (2014): 258. http://dx.doi.org/10.1071/rd12341.

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Increasing intracellular concentrations of cyclic adenosine monophosphate (cAMP) within the cumulus–oocyte complex (COC) inhibits or delays spontaneous oocyte maturation and improves the developmental competence of the oocyte in many species, but information for carnivores is limited. The objectives of the present study were to describe the effects of isobutyl methylxanthine (IBMX), which decreases cAMP degradation, and forskolin, which increases cAMP production, on spontaneous and induced maturation (by equine chorionic gonadotrophin (eCG) and epidermal growth factor (EGF)) of feline oocytes

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MILLS, Catherine, Elizabeth SUTTON, Julian KOPLIN, Ezra KNEEBONE, Karinne LUDLOW, and Ainsley NEWSON. "Mitochondrial Donation and Egg Donor Consent in Australia." Fertility & Reproduction 04, no. 03n04 (2022): 150. http://dx.doi.org/10.1142/s2661318222740620.

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Background: Legislation to permit mitochondrial donation (MD) in Australia was introduced into Federal Parliament in early 2021, and the techniques may be legalized and made available soon. MD enables women affected by disease-causing mutations in their mitochondrial DNA to have a genetically related child who is unlikely to inherit these mutations. MD relies on the donation of oocytes. Australia’s oocyte donation system does not meet current demand for oocytes and MD would add to this. Consequently, the implementation of MD would raise critical questions about the system of procuring donors a

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Dumesic, Daniel A., Annie A. Guedikian, Vanessa K. Madrigal, et al. "Cumulus Cell Mitochondrial Resistance to Stress In Vitro Predicts Oocyte Development During Assisted Reproduction." Journal of Clinical Endocrinology & Metabolism 101, no. 5 (2016): 2235–45. http://dx.doi.org/10.1210/jc.2016-1464.

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Abstract Context: Complex cumulus cell-oocyte interactions govern energy utilization during oocyte development. Objective: This study investigates the relationship of cumulus cell mitochondria with oocyte development during ovarian stimulation for in vitro fertilization (IVF). Design: This is a prospective cohort study. Setting: The setting was an academic center. Patients: Thirty women underwent ovarian stimulation for IVF. Intervention(s): Pooled cumulus cells were collected; numbers of total and mature oocytes and two-pronuclear (day 1), six- to eight-cell cleavage (day 3), and blastocyst (

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Yang, M., S. Hu, L. Cox, et al. "307 MATURATION RATE AND GENE EXPRESSION ANALYSIS OF GOAT OOCYTES SELECTED BY FOLLICLE SIZE AND BRILLIANT CRESYL BLUE STAINING." Reproduction, Fertility and Development 27, no. 1 (2015): 242. http://dx.doi.org/10.1071/rdv27n1ab307.

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Oocyte quality plays a critical role in determining the success of embryo development. Studies on cattle and goats indicate that oocytes derived from large follicles (LFO) have greater developmental competence than those derived from small follicles (SFO). Brilliant cresyl blue (BCB) staining determines the activity of glucose-6-phosphate dehydrogenase and is a commonly used noninvasive marker of oocyte competence. Studies in pigs, goats, cows, mice, and dogs showed that the maturation and blastocyst developmental rate of BCB+ oocytes is significantly higher than BCB– oocytes. The aim of this

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Cai, L., E. Kim, S. U. Hwang, et al. "156 EFFECTS OF MORPHOLOGY TYPE OF POLAR BODY ON PORCINE OOCYTE QUALITY AND DEVELOPMENTAL POTENTIAL AFTER IN VITRO FERTILIZATION." Reproduction, Fertility and Development 26, no. 1 (2014): 192. http://dx.doi.org/10.1071/rdv26n1ab156.

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Evaluation of morphology of first polar body (1st PB) could be a method for the oocyte's quality and developmental competence. The developmental potential of oocyte with fragmented PB after in vitro maturation (IVM) is a controversial issue. The aim of this study is to investigate the effects of PB morphology type on oocyte quality and developmental competence after IVF. Porcine ovaries were obtained from prepubertal gilts at a local slaughterhouse and transported to the laboratory within 2 h in physiological saline supplemented with 100 IU mL–1 penicillin G and 100 mg mL–1 streptomycin sulfat

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