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Dissertations / Theses on the topic 'Optimization of protease'

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Published: 6 September 2023

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1

Peterson, Shane. "Improved CoMFA Modeling by Optimization of Settings : Toward the Design of Inhibitors of the HCV NS3 Protease." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8140.

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2

De, Wet Tinus Andre. "Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71790.

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Thesis (MSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: This study describes: a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin b) Re-engineering / development of a new method for purifying trypsin and α-chymotrypsin that delivered higher product yields and improved control exercised over the process by investigating: i. Extraction methods ii. Centrifugation iii. Ultrafiltration iv. Chymotrypsinogen and trypsin crystallization v. Column chromatography vi. Investigation into different raw material source
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3

Wang, Liping. "Enhanced Production of Heterologous Protein by Recombinant Aspergillus niger Through Bioprocessing Strategies in Submerged Culture." Ohio University / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1038425153.

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4

Souza, Paula Monteiro de. "Produção de proteases por fungos filamentosos isolados do cerrado do centro-oeste brasileiro." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/9/9134/tde-15052015-134608/.

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Proteases ácidas pertencem a um importante grupo de enzimas industriais produzidas por fungos filamentosos, com aplicações na indústria de alimentos, de couro, farmacêutica e de cosméticos. O objetivo principal deste trabalho foi avaliar a produção de proteases ácidas extracelulares de fungos filamentosos isolados do solo do cerrado do centro-oeste brasileiro. Inicialmente, foi realizada uma triagem para avaliar a capacidade de 17 linhagens de fungos quanto à produção de protease em meio de cultura contendo Agar-leite. O fungo Aspergillus foetidus foi selecionado como melhor produtor de protea
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5

Bourscheid, Cristiane. "OTIMIZAÇÃO DO PROCESSO DE HIDRÓLISE ENZIMÁTICA DE COPRODUTO DA DESOSSA DE FRANGO E APLICAÇÃO DO HIDROLISADO EM HAMBÚRGUER." UNIVERSIDADE ESTADUAL DE PONTA GROSSA, 2015. http://tede2.uepg.br/jspui/handle/prefix/645.

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Made available in DSpace on 2017-07-21T18:53:04Z (GMT). No. of bitstreams: 1 CRISTIANE BOURSCHEID.pdf: 1448163 bytes, checksum: 21f79c9c1335f39830bfef99ee3f5f19 (MD5) Previous issue date: 2015-03-11<br>The chicken slaughterhouse generate co-products during slaughter, for example, feathers, bones, blood and guts, usually intended for animal feed with low added value. In bone part of the meat remains bound even after deboning and is a good source of substrate for the enzymatic hydrolysis. The protein hydrolysates may be applied as a nutritional supplement in foods. Given this context, the obje
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6

Stewart, Gaynelle. "Engineering Saccharomyces ceresisiae for the Secretion of an Extracellular Lipase." ScholarWorks@UNO, 2007. http://scholarworks.uno.edu/td/577.

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Developing microbial systems capable of converting low cost lipids into value added products depends on the ability to acquire substrates from the growth media. Saccharomyces cerevisiae can acquire free fatty acids from the growth media and a portion of these lipids can be converted into new lipid products. However, they cannot acquire complex lipids from the growth media unless a nonspecific lipase is included. To circumvent lipase addition, we are genetically engineering S. cerevisiae to secrete a lipase into the growth media. We selected the LIP2 gene from Yarrowia lipolytica, which encodes
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7

Lalanne, Jean-Benoît. "Multiscale dissection of bacterial proteome optimization." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/130217.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Physics, May, 2020<br>Cataloged from student-submitted PDF version of thesis.<br>Includes bibliographical references (pages 315-348).<br>The quantitative composition of proteomes results from biophysical and biochemical selective pressures acting under system-level resource allocation constraints. The nature and strength of these evolutionary driving forces remain obscure. Through the development of analytical tools and precision measurement platforms spanning biological scales, we found evidence of optimization in bacterial
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8

Oliveira, Cibele Freitas de. "Estudo da hidrólise da proteína de soja utilizando proteases de Chryseobacterium sp. para o uso como antioxidante em alimentos." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/61061.

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A demanda por antioxidantes naturais vem aumentando devido à toxicidade de alguns antioxidantes sintéticos. Estudos vêm identificando antioxidantes de origem natural, como a proteína da soja, que é capaz de contribuir na melhoria de propriedades funcionais e biológicas de alimentos. A hidrólise enzimática da proteína de soja aumenta sua atividade antioxidante, assim como a capacidade emulsificante e a capacidade de formação de espuma. O objetivo deste trabalho foi o estudo da hidrólise da proteína de soja através de uma protease produzida por Chryseobacterium sp., a verificação da capacidade a
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9

Myhara, Robert Michael. "Optimization of cultural factors influencing the production of extracellular vesicles and proteinase by Pseudomonas fragi ATCC 4973." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/29249.

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Pseudomonas fragi ATCC 4973 was grown in trypticase soy broth (TSB), on a trypticase soy broth + 1.5% agar (TSA) surface, and in a defined citrate broth. The citrate broth contained glutamine as the sole nitrogen source, Pseudomonas fragi grown in TSB started proteinase production at 24 h, during the late logarithmic early stationary growth phase. Pseudomonas fragi grown on TSA surfaces initiated proteinase production at 4 h, 20 hours earlier than in liquid medium. Electron micrographs of P. fragi grown on TSA revealed extracellular vesicles ca. 20 nm in diameter "blebbing" off the surface of
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10

Bayraktar, Eda. "Effects Of Ph On Human Growth Hormone Production By Pichia Pastoris Considering The Expression Levels Of Regulatory Genes." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12610882/index.pdf.

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In this study, the aim was to investigate the effects of pH on therapeutically important protein, recombinant human growth hormone (rhGH), production by Pichia pastoris considering the expression levels of regulatory genes. In this frame, firstly the host microorganism was selected between two different methanol utilization phenotypes of P. pastoris, Mut+ and MutS on media containing glycerol/methanol or sorbitol/methanol. The highest rhGH production, 120 g L-1, and hGH gene expression, 9.84x109 copies mg-1 CDW, were achieved in the medium containing 30 g L-1 sorbitol and 1% (v/v) methanol by
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11

Kocillari, Loren. "Variational principles and optimality in biological systems." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3425402.

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The aim of this thesis is to investigate the signatures of evolutionary optimization in biological systems, such as in proteins, human behaviours and transport tissues in vascular plants (xylems), by means of the Pareto optimality analysis and the calculus of variations. In the first part of this thesis, we address multi-objective optimization problems with tradeoffs through the Pareto optimality analysis ( [132],[69]), according which the best tradeoff solutions correspond to the optimal species, enclosed onto low-dimensional geometrical polytopes, defined as Pareto optimal fronts, in the spa
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12

Lee, Hyosung. "DEVELOPMENT OF NOVEL AHR ANTAGONISTS." UKnowledge, 2010. http://uknowledge.uky.edu/gradschool_diss/103.

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Aryl hydrocarbon receptor (AHR) is a sensor protein, activated by aromatic chemical species for transcriptionally regulating xenobiotic metabolizing enzymes. AHR is also known to be involved in a variety of pathogenesis such as cancer, diabetes mellitus, cirrhosis, asthma, etc. The AHR signaling induced by xenobiotics has been intensively studied whereas its physiological role in the absence of xenobiotics is poorly understood. Despite a number of ligands of AHR have been reported thus far, further applications are still hampered by the lack of specificity and/or the partially agonistic activi
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13

Borgiani, Edoardo. "Geometrical optimization of a short-stem hip implant for the reduction of proximal stress shielding (ottimizzazione geometrica di uno stelo corto di protesi d?anca per la riduzione della schermatura del carico prossimale)." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8581/.

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Nowadays the number of hip joints arthroplasty operations continues to increase because the elderly population is growing. Moreover, the global life expectancy is increasing and people adopt a more active way of life. For this reasons, the demand of implant revision operations is becoming more frequent. The operation procedure includes the surgical removal of the old implant and its substitution with a new one. Every time a new implant is inserted, it generates an alteration in the internal femur strain distribution, jeopardizing the remodeling process with the possibility of bone tissue loss.
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14

Midoux, Patrick. "Ciblage de cytotoxiques et d'immunostimulants par des anticorps monoclonaux et des neoglycoproteines." Orléans, 1987. http://www.theses.fr/1987ORLE2014.

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15

Penel, Simon. "Organisation du détergent dans les cristaux de protéines membranaires : analyse des cristaux des porines de Rhodobacter capsulatus et de Escherichia coli." Université Joseph Fourier (Grenoble), 1997. http://www.theses.fr/1997GRE10132.

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Le nombre de proteines membranaires dont la structure est connue est tres faible en regard du nombre de structures resolues, ceci en raison des difficultes a obtenir des cristaux de qualite suffisante pour les etudes par diffraction des rayons x. La finalite de ce travail est de comprendre l'implication du detergent, utilise pour solubiliser puis cristalliser les proteines membranaires, dans le processus de cristallisation et dans la qualite des cristaux obtenus. En particulier, on s'est interesse a l'organisation prise par le detergent dans les cristaux, et aux differentes interactions auxque
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16

Sidhu, Satinder K. "Optimization of In Vitro Cultures of Neonatal Porcine Islets Pre-transplantation." Master's thesis, 2009. http://hdl.handle.net/10048/489.

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Islet transplantation is an attractive method to achieve blood glucose homeostasis. However, β-cell function declines over time. Therefore, it is necessary to explore strategies to enhance the β-cell mass and function. Also, because there is a severe shortage of human cadaver tissue, alternative sources of insulin secreting tissue need to be examined. Neonatal porcine islet (NPI) tissue has emerged as an attractive alternative source of β-cells. The aim of this thesis was to optimize the culturing conditions of NPIs pre-transplantation so that the available tissue can be used as efficiently an
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17

Guo, Ya-Ting, and 郭雅婷. "Optimization on conditions for the hydrolysis of shrimp waste by protease and chitinase from Bacillus subtilis YJ1 and Development of probiotics fermented chitooligosaccharide beverages." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/02917216057794960159.

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碩士<br>國立高雄海洋科技大學<br>水產食品科學研究所<br>97<br>To produce high protease and chitinase activities, extra addition of chitins and CMC to the medium was conducted on the cultivation of Bacillus subtilis YJ-1. When this strain was incubated in a complex medium containing 0.5% (w/v) commercial chitin at 37oC, 150 rpm for 3 days, the highest neutral (655.2 U/mL•min) and alkaline (638.2 U/mL•min) proteases activities were obtained. The chitinases activities were 438.04 U/mL•min and 4.22 U/mL•min measured by neocuproine and DMBA methods, respectively. The shrimp shell solution was subjected to the hydrolysis
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