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Journal articles on the topic 'Optogenetic mapping'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Li, Gongxin, Jia Yang, Yuechao Wang, Wenxue Wang, and Lianqing Liu. "Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities." Nanoscale 10, no. 45 (2018): 21046–51. http://dx.doi.org/10.1039/c8nr05014g.

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2

Matsuzaki, Masanori. "Transcranial optogenetic mapping of the mouse motor cortex." Neuroscience Research 65 (January 2009): S19. http://dx.doi.org/10.1016/j.neures.2009.09.1594.

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Chen, Yi, Filip Sobczak, Patricia Pais-Roldán, Cornelius Schwarz, Alan P. Koretsky, and Xin Yu. "Mapping the Brain-Wide Network Effects by Optogenetic Activation of the Corpus Callosum." Cerebral Cortex 30, no. 11 (2020): 5885–98. http://dx.doi.org/10.1093/cercor/bhaa164.

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Abstract Optogenetically driven manipulation of circuit-specific activity enables causality studies, but its global brain-wide effect is rarely reported. Here, we applied simultaneous functional magnetic resonance imaging (fMRI) and calcium recording with optogenetic activation of the corpus callosum (CC) connecting barrel cortices (BC). Robust positive BOLD was detected in the ipsilateral BC due to antidromic activity, spreading to the ipsilateral motor cortex (MC), and posterior thalamus (PO). In the orthodromic target, positive BOLD was reliably evoked by 2 Hz light pulses, whereas 40 Hz li

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Lin, Gang, Wenyi Shi, Ningxia Zhang, Yi-Tsang Lee, Youjun Wang, and Ji Jing. "Proteomic mapping and optogenetic manipulation of membrane contact sites." Biochemical Journal 479, no. 17 (2022): 1857–75. http://dx.doi.org/10.1042/bcj20220382.

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Membrane contact sites (MCSs) mediate crucial physiological processes in eukaryotic cells, including ion signaling, lipid metabolism, and autophagy. Dysregulation of MCSs is closely related to various diseases, such as type 2 diabetes mellitus (T2DM), neurodegenerative diseases, and cancers. Visualization, proteomic mapping and manipulation of MCSs may help the dissection of the physiology and pathology MCSs. Recent technical advances have enabled better understanding of the dynamics and functions of MCSs. Here we present a summary of currently known functions of MCSs, with a focus on optical

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Niemeyer, James E. "Mapping whole brain seizure network recruitment with optogenetic kindling." Journal of Neurophysiology 127, no. 2 (2022): 393–96. http://dx.doi.org/10.1152/jn.00525.2021.

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Epilepsy is often labeled as a network disorder, though a common view of seizures holds that they initiate in a singular onset zone before expanding contiguously outward. A recent report by Choy et al. (Choy M, Dadgar-Kiani E, Cron GO, Duffy BA, Schmid F, Edelman BJ, Asaad M, Chan RW, Vahdat S, Lee JH. Neuron 2021 Oct 23: S0896-6273(21)00778-9.) leverages new tools to study whole brain dynamics during epileptic seizures originating in the hippocampus. Cell-type-specific kindling and functional imaging revealed how various brain regions were recruited to seizures and uncovered a novel form of m

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Hira, Riichiro, Fuki Okubo, Naoki Honkura, et al. "Transcranial optogenetic stimulation for mapping of the motor cortex." Neuroscience Research 65 (January 2009): S201. http://dx.doi.org/10.1016/j.neures.2009.09.1099.

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7

Wu, Ling, Ao Dong, Liting Dong, Shi-Qiang Wang, and Yulong Li. "PARIS, an optogenetic method for functionally mapping gap junctions." IBRO Reports 6 (September 2019): S392. http://dx.doi.org/10.1016/j.ibror.2019.07.1245.

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8

Zaglia, Tania, Nicola Pianca, Giulia Borile, et al. "Optogenetic determination of the myocardial requirements for extrasystoles by cell type-specific targeting of ChannelRhodopsin-2." Proceedings of the National Academy of Sciences 112, no. 32 (2015): E4495—E4504. http://dx.doi.org/10.1073/pnas.1509380112.

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Extrasystoles lead to several consequences, ranging from uneventful palpitations to lethal ventricular arrhythmias, in the presence of pathologies, such as myocardial ischemia. The role of working versus conducting cardiomyocytes, as well as the tissue requirements (minimal cell number) for the generation of extrasystoles, and the properties leading ectopies to become arrhythmia triggers (topology), in the normal and diseased heart, have not been determined directly in vivo. Here, we used optogenetics in transgenic mice expressing ChannelRhodopsin-2 selectively in either cardiomyocytes or the

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9

Schmid, Florian, Lydia Wachsmuth, Miriam Schwalm, et al. "Assessing sensory versus optogenetic network activation by combining (o)fMRI with optical Ca2+ recordings." Journal of Cerebral Blood Flow & Metabolism 36, no. 11 (2016): 1885–900. http://dx.doi.org/10.1177/0271678x15619428.

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Encoding of sensory inputs in the cortex is characterized by sparse neuronal network activation. Optogenetic stimulation has previously been combined with fMRI (ofMRI) to probe functional networks. However, for a quantitative optogenetic probing of sensory-driven sparse network activation, the level of similarity between sensory and optogenetic network activation needs to be explored. Here, we complement ofMRI with optic fiber-based population Ca2+ recordings for a region-specific readout of neuronal spiking activity in rat brain. Comparing Ca2+ responses to the blood oxygenation level-depende

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Stucynski, J., A. Schott, J. Baik, J. Hong, F. Weber, and S. Chung. "0074 Inhibitory Neurons in the Dorsomedial Medulla Promote REM Sleep." Sleep 43, Supplement_1 (2020): A30. http://dx.doi.org/10.1093/sleep/zsaa056.072.

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Abstract Introduction The neural circuits controlling rapid eye movement (REM) sleep, and in particular the role of the medulla in regulating this brain state, remains an active area of study. Previous electrophysiological recordings in the dorsomedial medulla (DM) and electrical stimulation experiments suggested an important role of this area in the control of REM sleep. However the identity of the involved neurons and their precise role in REM sleep regulation are still unclear. Methods The properties of DM GAD2 neurons in mice were investigated through stereotaxic injection of CRE-dependent

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11

Hira, Riichiro, Naoki Honkura, Jun Noguchi, et al. "Transcranial optogenetic stimulation for functional mapping of the motor cortex." Journal of Neuroscience Methods 179, no. 2 (2009): 258–63. http://dx.doi.org/10.1016/j.jneumeth.2009.02.001.

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12

Izquierdo-Serra, Mercè, Jan J. Hirtz, Ben Shababo, and Rafael Yuste. "Two-Photon Optogenetic Mapping of Excitatory Synaptic Connectivity and Strength." iScience 8 (October 2018): 15–28. http://dx.doi.org/10.1016/j.isci.2018.09.008.

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13

Linders, Louisa E., Laura F. Supiot, Wenjie Du, et al. "Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology." International Journal of Molecular Sciences 23, no. 19 (2022): 11612. http://dx.doi.org/10.3390/ijms231911612.

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Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such networks. Using this combination of methods, originally termed channelrhodopsin-assisted circuit mapping (CRACM), a multitude of measures of synaptic functioning can be taken. The current review discusses their rationale, current applications in the field, and their associated caveats. Specifically, the review addresses: (1) How to assess the

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14

Thanos, P. K., L. Robison, E. J. Nestler, et al. "Mapping Brain Metabolic Connectivity in Awake Rats with PET and Optogenetic Stimulation." Journal of Neuroscience 33, no. 15 (2013): 6343–49. http://dx.doi.org/10.1523/jneurosci.4997-12.2013.

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15

Jing, Ji, Lian He, Aomin Sun, et al. "Proteomic Mapping and Optogenetic Control of ER-PM Junctions in Living Cells." Biophysical Journal 110, no. 3 (2016): 258a. http://dx.doi.org/10.1016/j.bpj.2015.11.1415.

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16

Sans-Dublanc, Arnau, Anna Chrzanowska, Katja Reinhard, et al. "Optogenetic fUSI for brain-wide mapping of neural activity mediating collicular-dependent behaviors." Neuron 109, no. 11 (2021): 1888–905. http://dx.doi.org/10.1016/j.neuron.2021.04.008.

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17

Antin, Benjamin, Masato Sadahiro, Marta Gajowa, Marcus A. Triplett, Hillel Adesnik, and Liam Paninski. "Removing direct photocurrent artifacts in optogenetic connectivity mapping data via constrained matrix factorization." PLOS Computational Biology 20, no. 5 (2024): e1012053. http://dx.doi.org/10.1371/journal.pcbi.1012053.

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Monosynaptic connectivity mapping is crucial for building circuit-level models of neural computation. Two-photon optogenetic stimulation, when combined with whole-cell recording, enables large-scale mapping of physiological circuit parameters. In this experimental setup, recorded postsynaptic currents are used to infer the presence and strength of connections. For many cell types, nearby connections are those we expect to be strongest. However, when the postsynaptic cell expresses opsin, optical excitation of nearby cells can induce direct photocurrents in the postsynaptic cell. These photocur

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18

Leong, Alex T. L., Yong Gu, Ying-Shing Chan, et al. "Optogenetic fMRI interrogation of brain-wide central vestibular pathways." Proceedings of the National Academy of Sciences 116, no. 20 (2019): 10122–29. http://dx.doi.org/10.1073/pnas.1812453116.

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Blood oxygen level-dependent functional MRI (fMRI) constitutes a powerful neuroimaging technology to map brain-wide functions in response to specific sensory or cognitive tasks. However, fMRI mapping of the vestibular system, which is pivotal for our sense of balance, poses significant challenges. Physical constraints limit a subject’s ability to perform motion- and balance-related tasks inside the scanner, and current stimulation techniques within the scanner are nonspecific to delineate complex vestibular nucleus (VN) pathways. Using fMRI, we examined brain-wide neural activity patterns elic

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19

Ebina, Teppei, Keitaro Obara, Akiya Watakabe, et al. "Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset." Proceedings of the National Academy of Sciences 116, no. 45 (2019): 22844–50. http://dx.doi.org/10.1073/pnas.1903445116.

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Optogenetics is now a fundamental tool for investigating the relationship between neuronal activity and behavior. However, its application to the investigation of motor control systems in nonhuman primates is rather limited, because optogenetic stimulation of cortical neurons in nonhuman primates has failed to induce or modulate any hand/arm movements. Here, we used a tetracycline-inducible gene expression system carrying CaMKII promoter and the gene encoding a Channelrhodopsin-2 variant with fast kinetics in the common marmoset, a small New World monkey. In an awake state, forelimb movements

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Entcheva, Emilia, and Gil Bub. "All-optical control of cardiac excitation: combined high-resolution optogenetic actuation and optical mapping." Journal of Physiology 594, no. 9 (2016): 2503–10. http://dx.doi.org/10.1113/jp271559.

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21

Urstadt, Kevin R., and Kent C. Berridge. "Optogenetic mapping of feeding and self-stimulation within the lateral hypothalamus of the rat." PLOS ONE 15, no. 1 (2020): e0224301. http://dx.doi.org/10.1371/journal.pone.0224301.

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22

Kressel, Adam M., Tea Tsaava, Yaakov A. Levine, et al. "Identification of a brainstem locus that inhibits tumor necrosis factor." Proceedings of the National Academy of Sciences 117, no. 47 (2020): 29803–10. http://dx.doi.org/10.1073/pnas.2008213117.

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In the brain, compact clusters of neuron cell bodies, termed nuclei, are essential for maintaining parameters of host physiology within a narrow range optimal for health. Neurons residing in the brainstem dorsal motor nucleus (DMN) project in the vagus nerve to communicate with the lungs, liver, gastrointestinal tract, and other organs. Vagus nerve-mediated reflexes also control immune system responses to infection and injury by inhibiting the production of tumor necrosis factor (TNF) and other cytokines in the spleen, although the function of DMN neurons in regulating TNF release is not known

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23

Kim, Jinsook, Soojung Lee, Sachiko Tsuda, et al. "Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses." Cell Reports 7, no. 5 (2014): 1601–13. http://dx.doi.org/10.1016/j.celrep.2014.04.047.

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24

Honda, Takato. "Optogenetic and thermogenetic manipulation of defined neural circuits and behaviors in Drosophila." Learning & Memory 29, no. 4 (2022): 100–109. http://dx.doi.org/10.1101/lm.053556.121.

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Neural network dynamics underlying flexible animal behaviors remain elusive. The fruit fly Drosophila melanogaster is considered an excellent model in behavioral neuroscience because of its simple neuroanatomical architecture and the availability of various genetic methods. Moreover, Drosophila larvae's transparent body allows investigators to use optical methods on freely moving animals, broadening research directions. Activating or inhibiting well-defined events in excitable cells with a fine temporal resolution using optogenetics and thermogenetics led to the association of functions of def

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25

Lee, Wonryung, Dongmin Kim, Naoji Matsuhisa, et al. "Transparent, conformable, active multielectrode array using organic electrochemical transistors." Proceedings of the National Academy of Sciences 114, no. 40 (2017): 10554–59. http://dx.doi.org/10.1073/pnas.1703886114.

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Mechanically flexible active multielectrode arrays (MEA) have been developed for local signal amplification and high spatial resolution. However, their opaqueness limited optical observation and light stimulation during use. Here, we show a transparent, ultraflexible, and active MEA, which consists of transparent organic electrochemical transistors (OECTs) and transparent Au grid wirings. The transparent OECT is made of Au grid electrodes and has shown comparable performance with OECTs with nontransparent electrodes/wirings. The transparent active MEA realizes the spatial mapping of electrocor

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26

Mahoney, C. E., W. Zhao, A. Coffey, C. Woods, D. Kroeger, and T. Scammell. "0005 Cataplexy Triggered by Social Cues: A Role for Oxytocin in the Amygdala." Sleep 43, Supplement_1 (2020): A2. http://dx.doi.org/10.1093/sleep/zsaa056.004.

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Abstract Introduction People with narcolepsy type 1 report that cataplexy is triggered most often by positive social experiences such as laughing with friends, yet the mechanisms through which social interaction promotes cataplexy are unknown. We hypothesize a subpopulation of central amygdala neurons that are sensitive to the prosocial neuropeptide, oxytocin (CeAOTR), respond to positive valence and trigger cataplexy. Methods We have used in vivo calcium imaging, chemogenetic and optogenetic approaches to characterize the activity pattern of these neurons and to manipulate their activity stat

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Guragain, Bijay, Hanyu Zhang, Yalin Wu, et al. "Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids." Journal of Molecular and Cellular Cardiology 199 (February 2025): 51–59. https://doi.org/10.1016/j.yjmcc.2024.12.003.

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Park, Ah Hyung, Seung Hyun Lee, Changju Lee, et al. "Optogenetic Mapping of Functional Connectivity in Freely Moving Mice via Insertable Wrapping Electrode Array Beneath the Skull." ACS Nano 10, no. 2 (2016): 2791–802. http://dx.doi.org/10.1021/acsnano.5b07889.

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29

Yang, Fangjing, Fei Wang, Xingyi Ma, Mingjie Zhou, Su Jiang, and Wendong Xu. "Longitudinal optogenetic mapping reveals enhanced motor control by the contralesional cortex after traumatic brain injury in mice." Experimental Neurology 369 (November 2023): 114546. http://dx.doi.org/10.1016/j.expneurol.2023.114546.

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Bauer, Adam. "Optogenetic motor mapping in awake mice reveals that ethological-like behaviors are topographically organized on the cortex." Brain Stimulation 18, no. 1 (2025): 616–17. https://doi.org/10.1016/j.brs.2024.12.1163.

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Park, Sung Il, Gunchul Shin, Jordan G. McCall, et al. "Stretchable multichannel antennas in soft wireless optoelectronic implants for optogenetics." Proceedings of the National Academy of Sciences 113, no. 50 (2016): E8169—E8177. http://dx.doi.org/10.1073/pnas.1611769113.

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Optogenetic methods to modulate cells and signaling pathways via targeted expression and activation of light-sensitive proteins have greatly accelerated the process of mapping complex neural circuits and defining their roles in physiological and pathological contexts. Recently demonstrated technologies based on injectable, microscale inorganic light-emitting diodes (μ-ILEDs) with wireless control and power delivery strategies offer important functionality in such experiments, by eliminating the external tethers associated with traditional fiber optic approaches. Existing wireless μ-ILED embodi

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Lim, Diana H., Jeffrey M. LeDue, Majid H. Mohajerani, and Timothy H. Murphy. "Optogenetic Mapping after Stroke Reveals Network-Wide Scaling of Functional Connections and Heterogeneous Recovery of the Peri-Infarct." Journal of Neuroscience 34, no. 49 (2014): 16455–66. http://dx.doi.org/10.1523/jneurosci.3384-14.2014.

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Xu, Xiangmin, Taruna Ikrar, Yanjun Sun, et al. "High-resolution and cell-type-specific photostimulation mapping shows weak excitatory vs. strong inhibitory inputs in the bed nucleus of the stria terminalis." Journal of Neurophysiology 115, no. 6 (2016): 3204–16. http://dx.doi.org/10.1152/jn.01148.2015.

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The bed nucleus of the stria terminalis (BNST) is a key component of the extended amygdala and has been implicated in anxiety and addiction. As individual neurons function within neural circuits, it is important to understand local microcircuits and larger network connections of identified neuronal types and understand how maladaptive changes in the BNST neural networks are induced by stress and drug abuse. However, due to limitations of classic anatomical and physiological methods, the local circuit organization of synaptic inputs to specific BNST neuron types is not well understood. In this

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Qian, Jun, Wei Wu, Wenhui Xiong, Zhi Chai, Xiao-Ming Xu, and Xiaoming Jin. "Longitudinal Optogenetic Motor Mapping Revealed Structural and Functional Impairments and Enhanced Corticorubral Projection after Contusive Spinal Cord Injury in Mice." Journal of Neurotrauma 36, no. 3 (2019): 485–99. http://dx.doi.org/10.1089/neu.2018.5713.

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35

Troise, Luca, Nikolaj Winther Hansen, Christoffer Olsson, et al. "In vitro recording of muscle activity induced by high intensity laser optogenetic stimulation using a diamond quantum biosensor." AVS Quantum Science 4, no. 4 (2022): 044402. http://dx.doi.org/10.1116/5.0106099.

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The detection of physiological activity at the microscopic level is key for understanding the function of biosystems and relating this to their physical structure. Current sensing methods for in vitro study of living tissue often rely on invasive probes to stimulate and detect activity, bearing the risk of inducing damage in the target system. In recent years, a new type of quantum sensor based on color centers in diamond has begun to offer the possibility to instead passively sense and image living biological systems. Here, we use such a sensor to realize the recording of the biomagnetic fiel

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Gupta, Pallavi, Nandhini Balasubramaniam, Hwan-You Chang, Fan-Gang Tseng, and Tuhin Subhra Santra. "A Single-Neuron: Current Trends and Future Prospects." Cells 9, no. 6 (2020): 1528. http://dx.doi.org/10.3390/cells9061528.

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The brain is an intricate network with complex organizational principles facilitating a concerted communication between single-neurons, distinct neuron populations, and remote brain areas. The communication, technically referred to as connectivity, between single-neurons, is the center of many investigations aimed at elucidating pathophysiology, anatomical differences, and structural and functional features. In comparison with bulk analysis, single-neuron analysis can provide precise information about neurons or even sub-neuron level electrophysiology, anatomical differences, pathophysiology,

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Li, Qince, KahYong Goh, Wei Kong, Ruby R. Ni, Vladimir Fast, and Lufang Zhou. "Abstract 38: Simultaneous Optical Pacing and Optical Voltage Mapping in Optogenetic Neonatal Rat Ventricular Myocyte Cultures." Circulation Research 117, suppl_1 (2015). http://dx.doi.org/10.1161/res.117.suppl_1.38.

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Optogenetics is an emerging technology allowing remote and precise control of cell activity in living tissues. Despite its rapid advancements, application of this innovative technology to cardiovascular research is still limited, in part due to shortage of optogenetic cardiac tissue models and compatible imaging methods. The present study aimed to develop an optogenetic culture model using neonatal rat ventricular myocytes (NRVM) expressing light-gated Channelrhodopsin-2 (ChR2) and characterize activation spread during optical stimulation using optical mapping of membrane potential (Vm). Prima

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Guragain, Bijay, Hanyu Zhang, Yalin Wu, et al. "Abstract 4139301: Simultaneous Optogenetic Stimulation, Voltage, and Calcium Mapping of Human Engineered Cardiac Tissue." Circulation 150, Suppl_1 (2024). http://dx.doi.org/10.1161/circ.150.suppl_1.4139301.

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Introduction: Optogenetics can precisely stimulate cardiac tissue, while optical mapping of voltage (Vm) and calcium transients (CaT) can image electrophysiological function with high spatiotemporal resolution. Here, we present a novel method that combines optogenetic actuation with dual Vm/CaT optical mapping. The optogenetic channels and CaT indicator are genetically encoded, while the Vm indicator is an organic dye. Methods: For model tissue, we used cardiac spheroid (600-800 µm in diameter) comprising cardiomyocytes and cardiac fibroblasts that were differentiated from human induced plurip

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Rieger, Michael, Christian Dellenbach, Johannes vom Berg, Jane Beil-Wagner, Ange Maguy, and Stephan Rohr. "Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation." Nature Communications 12, no. 1 (2021). http://dx.doi.org/10.1038/s41467-021-26039-8.

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AbstractDuring the last decade, cardiac optogenetics has turned into an essential tool for investigating cardiac function in general and for assessing functional interactions between different myocardial cell types in particular. To advance exploitation of the unique research opportunities offered by this method, we develop a panoramic opto-electrical measurement and stimulation (POEMS) system for mouse hearts. The core of the experimental platform is composed of 294 optical fibers and 64 electrodes that form a cup which embraces the entire ventricular surface of mouse hearts and enables strai

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Gruber, Amit, Oded Edri, Shany Glatstein, et al. "Optogenetic Control of Human Induced Pluripotent Stem Cell‐Derived Cardiac Tissue Models." Journal of the American Heart Association 11, no. 4 (2022). http://dx.doi.org/10.1161/jaha.121.021615.

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Background Optogenetics, using light‐sensitive proteins, emerged as a unique experimental paradigm to modulate cardiac excitability. We aimed to develop high‐resolution optogenetic approaches to modulate electrical activity in 2‐ and 3‐dimensional cardiac tissue models derived from human induced pluripotent stem cell (hiPSC)‐derived cardiomyocytes. Methods and Results To establish light‐controllable cardiac tissue models, opsin‐carrying HEK293 cells, expressing the light‐sensitive cationic‐channel CoChR, were mixed with hiPSC‐cardiomyocytes to generate 2‐dimensional hiPSC‐derived cardiac cell‐

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Zhang, Jia, Dan Chen, Patrick Sweeney, and Yunlei Yang. "An excitatory ventromedial hypothalamus to paraventricular thalamus circuit that suppresses food intake." Nature Communications 11, no. 1 (2020). http://dx.doi.org/10.1038/s41467-020-20093-4.

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AbstractIt is well recognized that ventromedial hypothalamus (VMH) serves as a satiety center in the brain. However, the feeding circuit for the VMH regulation of food intake remains to be defined. Here, we combine fiber photometry, chemo/optogenetics, virus-assisted retrograde tracing, ChR2-assisted circuit mapping and behavioral assays to show that selective activation of VMH neurons expressing steroidogenic factor 1 (SF1) rapidly inhibits food intake, VMH SF1 neurons project dense fibers to the paraventricular thalamus (PVT), selective chemo/optogenetic stimulation of the PVT-projecting SF1

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Chen, Fu-Der, Ankita Sharma, Tianyuan Xue, et al. "Implantable silicon neural probes with nanophotonic phased arrays for single-lobe beam steering." Communications Engineering 3, no. 1 (2024). https://doi.org/10.1038/s44172-024-00328-8.

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AbstractIn brain activity mapping with optogenetics, patterned illumination is crucial for targeted neural stimulation. However, due to optical scattering in brain tissue, light-emitting implants are needed to bring patterned illumination to deep brain regions. A promising solution is silicon neural probes with integrated nanophotonic circuits that form tailored beam patterns without lenses. Here we propose neural probes with grating-based light emitters that generate a single steerable beam. The light emitters, optimized for blue or amber light, combine end-fire optical phased arrays with sla

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Lim, Diana H., Jeffrey LeDue, Majid H. Mohajerani, Matthieu P. Vanni, and Timothy H. Murphy. "Optogenetic approaches for functional mouse brain mapping." Frontiers in Neuroscience 7 (2013). http://dx.doi.org/10.3389/fnins.2013.00054.

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44

Haar, Lauren, William Kinney, and David Lawrence. "Mapping myocyte microdomains with optogenetic cAMP signaling." FASEB Journal 36, S1 (2022). http://dx.doi.org/10.1096/fasebj.2022.36.s1.r2537.

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45

Pi, Chenghui, Wenjing Tang, Zhishuai Li, et al. "Cortical pain induced by optogenetic cortical spreading depression: from whole brain activity mapping." Molecular Brain 15, no. 1 (2022). http://dx.doi.org/10.1186/s13041-022-00985-w.

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Abstract Background Cortical spreading depression (CSD) is an electrophysiological event underlying migraine aura. Traditional CSD models are invasive and often cause injuries. The aim of the study was to establish a minimally invasive optogenetic CSD model and identify the active networks after CSD using whole-brain activity mapping. Methods CSD was induced in mice by light illumination, and their periorbital thresholds and behaviours in the open field, elevated plus-maze and light-aversion were recorded. Using c-fos, we mapped the brain activity after CSD. The whole brain was imaged, reconst

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Baines, Olivia, Rina Sha, Manish Kalla, et al. "Optical Mapping and Optogenetics in Cardiac Electrophysiology Research and Therapy: A State-of-the-Art Review." Europace, January 16, 2024. http://dx.doi.org/10.1093/europace/euae017.

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Abstract State-of-the-art innovations in optical cardiac electrophysiology are significantly enhancing cardiac research. A potential leap into patient care is now on the horizon. Optical mapping, using fluorescent probes and high-speed cameras, offers detailed insights into cardiac activity and arrhythmias by analysing electrical signals, calcium dynamics, and metabolism. Optogenetics utilises light-sensitive ion channels and pumps to realise contactless, cell-selective cardiac actuation for modelling arrhythmia, restoring sinus rhythm, and probing complex cell-cell interactions. The merging o

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Wu, Ling, Ao Dong, Liting Dong, Shi-Qiang Wang, and Yulong Li. "PARIS, an optogenetic method for functionally mapping gap junctions." eLife 8 (January 14, 2019). http://dx.doi.org/10.7554/elife.43366.

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Cell-cell communication via gap junctions regulates a wide range of physiological processes by enabling the direct intercellular electrical and chemical coupling. However, the in vivo distribution and function of gap junctions remain poorly understood, partly due to the lack of non-invasive tools with both cell-type specificity and high spatiotemporal resolution. Here, we developed PARIS (pairing actuators and receivers to optically isolate gap junctions), a new fully genetically encoded tool for measuring the cell-specific gap junctional coupling (GJC). PARIS successfully enabled monitoring o

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Glatstein, Shany, Matteo Ghiringhelli, yehuda wexler, Lilac Haimovich-Caspi, Irit Huber, and Lior Gepstein. "Abstract 18867: A Novel Light-Sensitive Ex-Vivo Rat Atrial Anatomical Model for Studying Atrial Arrhythmias and Cardioversion." Circulation 148, Suppl_1 (2023). http://dx.doi.org/10.1161/circ.148.suppl_1.18867.

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Introduction: Atrial arrhythmias, including atrial fibrillation (AF), contribute to significant morbidity and mortality. However, the lack of suitable tissue models in small animals hampers our understanding of the underlying mechanisms. Optogenetic actuators offer precise and reversible stimulation with high spatiotemporal resolution, presenting a unique opportunity to investigate atrial arrhythmias. Hypothesis: Our aim was to develop a light-sensitive ex-vivo anatomical model in rats for studying atrial arrhythmias and to utilize this model for optimizing optogenetic stimulation and cardiove

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Chen, Fu Der, Ankita Sharma, David A. Roszko, et al. "Development of wafer-scale multifunctional nanophotonic neural probes for brain activity mapping." Lab on a Chip, 2024. http://dx.doi.org/10.1039/d3lc00931a.

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Optical techniques, such as optogenetic stimulation and functional fluorescence imaging, have been revolutionary for neuroscience by enabling neural circuit analysis with cell-type specificity. To probe deep brain regions, implantable light...

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Mochizuki, Takanori, Satoshi Manita, Hiroshi Shimura, et al. "Optogenetic stimulation of neurons in the anterior cingulate cortex induces changes in intravesical bladder pressure and the micturition reflex." Scientific Reports 14, no. 1 (2024). http://dx.doi.org/10.1038/s41598-024-56806-8.

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AbstractLower urinary tract (LUT) function is controlled by the central nervous system, including higher-order cognitive brain regions. The anterior cingulate cortex (ACC) is one of these regions, but the role of its activity in LUT function remains poorly understood. In the present study, we conducted optogenetic experiments to manipulate neural activity in mouse ACC while monitoring bladder pressure to elucidate how the activity of ACC regulates LUT function. Selective optogenetic stimulation of excitatory neurons in ACC induced a sharp increase in bladder pressure, whereas activation of inh

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