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1
Bae, Sang Mu, Yujin Nam, and Byoung Ohan Shim. "Feasibility Study of Ground Source Heat Pump System Considering Underground Thermal Properties." Energies 11, no. 7 (July 10, 2018): 1786. http://dx.doi.org/10.3390/en11071786.
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: A typical ground source heat pump (GSHP) system in South Korea has a ground heat exchanger (GHX) with a length of 100–150 m, which utilizes annually stable underground temperature to meet the loads of cooling, heating and hot water in buildings. However, most GSHP systems have been introduced in heating dominated areas because the system performance advantage is larger compared with air source heat pump system than that in cooling dominated areas. To effectively provide geothermal energy to the building in the limited urban area, it is necessary to install deep GHXs. Despite its large capacity, there are few studies on GSHP system with deep GHX over 300 m. In this study, to estimate the performance of the GSHP system with deep GHX and evaluate its feasibility, numerical simulation was conducted. To quantitatively analyze heat transfer between soil and GHX, the coupled model with GHX model and ground heat and groundwater transfer model was used. Furthermore, the heat exchange rate and the source temperature were calculated according to the operation modes, the length of GHX, and soil conditions such as geothermal gradient and thermal conductivity. As a result, the total heat exchange rate of GHX with a length of 300 m heat exchanger was 12.62 kW, 173% that of a length of 150 m. Finally, it was found that the GSHP system with deep GHX has realistic possibility in good condition of geothermal gradient.
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Kim, Kwonye, Jaemin Kim, Yujin Nam, Euyjoon Lee, Eunchul Kang, and Evgueniy Entchev. "Analysis of Heat Exchange Rate for Low-Depth Modular Ground Heat Exchanger through Real-Scale Experiment." Energies 14, no. 7 (March 29, 2021): 1893. http://dx.doi.org/10.3390/en14071893.
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A ground source heat pump system is a high-performance technology used for maintaining a stable underground temperature all year-round. However, the high costs for installation, such as for boring and drilling, is a drawback that prevents the system to be rapidly introduced into the market. This study proposes a modular ground heat exchanger (GHX) that can compensate for the disadvantages (such as high-boring/drilling costs) of the conventional vertical GHX. Through a real-scale experiment, a modular GHX was manufactured and buried at a depth of 4 m below ground level; the heat exchange rate and the change in underground temperatures during the GHX operation were tracked and calculated. The average heat exchanges rate was 78.98 W/m and 88.83 W/m during heating and cooling periods, respectively; the underground temperature decreased by 1.2 °C during heat extraction and increased by 4.4 °C during heat emission, with the heat pump (HP) working. The study showed that the modular GHX is a cost-effective alternative to the vertical GHX; further research is needed for application to actual small buildings.
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Oh, Hyunjun, and James M. Tinjum. "Modeling of shallow, horizontal, unsaturated, ground-based heat exchangers with consideration of dry zone formation." E3S Web of Conferences 205 (2020): 07005. http://dx.doi.org/10.1051/e3sconf/202020507005.
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High fluid temperatures in ground-based heat exchangers (GHXs) during the cooling season may result in a decrease in thermal conductivity of adjacent backfill (λbackfill), potentially causing degradation in the performance efficiency of the GHX system. In this study, numerical modeling was performed using the SVOffice™ finite-element software to evaluate coupled heat and moisture transfer around two GHXs. Constant-temperature boundary conditions of 35 ºC were applied to the GHX surfaces, and thermal properties of the backfill were controlled for comparisons. For estimate typical hydraulic and thermal properties for the modeling, laboratory tests and literature review were performed. Modeling results indicate that coupled heat and moisture transfer occurs rapidly near the GHX involving a dry zone formation when λbackfill decreases. A boundary between dry and wet zones where soil thermal properties rapidly change was observed around 50% GHX temperature dissipated (T50), and accordingly T50 was used to optimize the pipe configuration. Coupled heat and moisture transfer increased when the GHX configurations were optimized with consideration of dry zone formation. These results imply that thermally enhanced, engineered backfill and optimized configurations can enhance GHX system efficiency.
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Oh, Hyunjun, and James M. Tinjum. "Simplified two-step, cross-sectional approach for coupled heat and moisture transfer modeling of shallow, horizontal, ground-based heat Exchangers." E3S Web of Conferences 205 (2020): 07004. http://dx.doi.org/10.1051/e3sconf/202020507004.
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This study evaluates a two-step, cross-sectional approach for designing shallow, unsaturated, horizontal, ground-based heat exchangers (GHXs). Numerical modeling was conducted for coupled heat and moisture transfer around GHXs under transient climatological conditions through a cooling season. Soil samples were collected in Texas and Wisconsin to measure thermal conductivity dry-out curves and soil-water characteristic curves for use in the modeling. Average daily meteorological data from central Texas was applied for the top boundary condition. Heat generation from the GHX was calculated by total condenser heat rejection from an environmental control unit based on ambient temperatures and unit specifications. In the first step of the modeling, results showed that the fluid temperature through the GHX loop was approximately 43 ºC, and rapid heat and moisture fluxes were observed around the GHX loops. High moisture flux along the upper surface was also observed due to high ambient temperatures that occur during the summer season. Using these results, exiting temperature of the GHX was estimated for the second cross-sectional modeling step. This two-step, cross-sectional modeling approach provides a systematic analysis of coupled heat and moisture transfer around shallow, horizontal, unsaturated GHX loops, thus simplifying high computational effort needed for full three-dimensional modeling of shallow GHX systems.
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Bae, Sang, Yujin Nam, Jong Choi, Kwang Lee, and Jae Choi. "Analysis on Thermal Performance of Ground Heat Exchanger According to Design Type Based on Thermal Response Test." Energies 12, no. 4 (February 18, 2019): 651. http://dx.doi.org/10.3390/en12040651.
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A ground source heat pump (GSHP) system has higher performance than air source heat pump system due to the use of more efficient ground heat source. However, the GSHP system performance depends on ground thermal properties and groundwater conditions. There are many studies on the improvement of GSHP system by developing ground heat exchanger (GHX) and heat exchange method. Several studies have suggested methods to improve heat exchange rate for the development of GHX. However, few real-scale experimental studies have quantitatively analyzed their performance using the same ground conditions. Therefore, the objective of this study was to evaluate the thermal performance of various pipe types of GHX by the thermal response test (TRT) under the same field and test conditions. Four kinds of GHX (HDPE type, HDPE-nano type, spiral fin type, and coaxial type) were constructed in the same site. Inlet and outlet temperatures of GHXs and effective thermal conductivity were measured through the TRT. In addition, the borehole thermal resistance was calculated to comparatively analyze the correlation of the heat exchange performance with each GHX. Result of the TRT revealed that averages effective thermal conductivities of HDPE type, HDPE-nano, spiral fin type, and coaxial type GHX were 2.25 W/m·K, 2.34 W/m·K, 2.55 W/m·K, and 2.16 W/m·K, respectively. In the result, it was found that the average borehole thermal resistance can be an important factor in TRT, but the effect of increased thermal conductivity of pipe material itself was not significant.
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Ratnasari, Agnes, Efri Efri, Muhammad Syamsoel Hadi, and Hasriadi Mat Akin. "KETAHANAN BEBERAPA GENOTIPE SORGUM (Sorghum bicolor [L]Moench) TERHADAP PENYAKIT ANTRAKNOSA (Colletotrichum graminicola) PADA DUA SISTEM POLA TANAM BERBEDA." Jurnal Agrotek Tropika 7, no. 2 (May 3, 2019): 351. http://dx.doi.org/10.23960/jat.v7i2.3258.
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Tujuan penelitian ini yaitu mengetahui ketahanan 15 genotipe sorgum yang ditanam pada dua sistem tanam berbeda yaitu monokultur dan tumpangsari. Penelitian ini dilaksanakan pada bulan April 2017- Februari 2018 di Desa Sukanegara, Kecamatan Tanjung Bintang, Kabupaten Lampung Selatan, Lampung dan di Laboratorium Hama dan Penyakit Tanaman Fakultas Pertanian Universitas Lampung. Perlakuan disusunmenggunakan rancangan acak kekompok dalam Split Plot Design dengan faktor utama adalah sistem pola tanam (tumpangsari, monokultur), dan anak petak adalah 15 genotipe sorgum (Numbu, Samurai 1, GH3, UPCA, GH4, P/I WHP, GH6, Super 2, GH13, P/F 51-93-C, Super 1, GH5, Mandau, GH7 dan TalagaBodas). Monokultur sorgum ditanam pada jarak 80 cm x 20 cm. Tumpangsari sorgum ubikayu dilakukan dengan cara menanam sorgum di antara tanaman ubikayusehingga jarak tanam sorgum tetap 80 cm x 20 cm, sedangkan jarak tanam ubikayu 80 cm x 60 cm, baik sorgum maupun ubikayu ditanam secara bersamaan. Hasil penelitian menunjukkan bahwa sistem tanam tumpangsari lebih efektif untuk menekan intensitas penyakit antraknosa. Pada penelitian ini intensitas penyakit antraknosa terhadap 15 genotipe sorgum yang diamati dikelompokan menjadi 3 kategori yaitu tinggi, sedang dan rendah. Genotipe Numbu, GH3, Talaga Bodas, Super 1, dan Mandau adalah genotipe dengan intensitas penyaki terendah dibandingkan genotipe Samurai 1, UPCA, GH4, P/I WHP, GH13, P/F 5-193-C, GH5, GH6 dan GH7 . Genotipe Samurai 1, UPCA, GH4, P/I WHP, GH13, P/F 5-193-C, GH5, GH6 dan GH7 adalah genotipe yang intensitas penyakitnya lebih rendah dibandingkan genotipe Super 2. Dan genotipe Super 2 adalah genotipe dengan intnsitas penyakit antraknosa tertinggi.
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Kim, Jaemin, and Yujin Nam. "Development of the Performance Prediction Equation for a Modular Ground Heat Exchanger." Energies 13, no. 22 (November 17, 2020): 6005. http://dx.doi.org/10.3390/en13226005.
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Although ground source heat pump (GSHP) systems are more efficient than conventional air source heat pump (ASHP) systems, their high initial investment cost makes it difficult to introduce them into small buildings. Therefore, the development of a method for reducing the installation costs of GSHPs for small buildings is essential. This study proposes a modular ground heat exchanger (GHX) for cost reduction and an improved workability of GSHPs. In addition, a numerical model was constructed for the analysis of the performance of the modular GHX. However, to easily introduce the new GHX at the building design stage, the development of a performance prediction method for the introduction of modular GHXs to small buildings is necessary. Therefore, the entering water temperature (EWT) equation was derived from the calculation methods in the heat transfer process, and the ground temperature model was developed in consideration of the operation condition. The numerical results showed that the average values of EWT and ground temperature were 8.11 °C and 8.00 °C, respectively under an average ambient temperature of 0.42 °C. In addition, the performance prediction model was compared with the numerical results. The results showed that the coefficient of variation of the root mean square error (RMSE) of the ground temperature and EWT model were 5.20% and 1.33%, respectively.
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Chang, Keun Sun, Min Jun Kim, and Young Jae Kim. "An Experimental Study on the Thermal Performance Evaluation of SCW Ground Heat Exchanger." International Journal of Air-Conditioning and Refrigeration 25, no. 01 (March 2017): 1750006. http://dx.doi.org/10.1142/s2010132517500067.
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In recent years, application of the standing column well (SCW) ground heat exchanger (GHX) has been noticeably increased as a heat transfer mechanism of ground source heat pump (GSHP) systems with its high heat capacity and efficiency. Determination of the ground thermal properties is an important task for sizing and estimating cost of the GHX. In this study, an in situ thermal response test (TRT) is applied to the thermal performance evaluation of SCW. Two SCWs with different design configurations are installed in sequence to evaluate their effects on the thermal performance of SCW using a single borehole. A line source method is used to derive the effective thermal conductivity and borehole thermal resistance. Effects of operating parameters are also investigated including bleed, heat injection rate, flow rate and filler height. Results show that the effective thermal conductivity of top drawn SCW (Type A) is 11.7% higher than that of bottom drawn SCW (Type B) and of operating parameters tested bleed is the most significant one for the improvement of the thermal performance (40.4% enhanced in thermal conductivity with 10.9% bleed).
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Muli, Farastika Unjunan, Efri Efri, Muhammad Syamsoel Hadi, and Radix Suharjo. "PENGARUH PENAMBAHAN PUPUK MIKRO DAN BEBERAPA GENOTIPE SORGUM (Sorghum bicolor (L.) Moench) TERHADAP INTENSITAS PENYAKIT ANTRAKNOSA (Colletotrichum sp.) DI LAHAN PETANI TANJUNG BINTANG LAMPUNG SELATAN." Jurnal Agrotek Tropika 8, no. 2 (May 20, 2020): 347. http://dx.doi.org/10.23960/jat.v8i2.3902.
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One of the diseases that often found in sorghum plants is anthracnose disease caused by Colletotrichum sp. The purposed of this study was to determine the effect of adding micro fertilizer and the use of several sorghum genotypes on the intensity of anthracnose disease. The study was conducted in Sukanegara, Tanjung Bintang, South Lampung in April 2017 - February 2018 and at the Laboratory of Plant Diseases and Pests, Faculty of Agriculture, University of Lampung. The treatments were arranged using a randomized block design in a split plot design (3 times replications), the main plot was micro nutrients (with micro addition and without micro addition) and 15 subgroups of sorghum genotypes (Numbu, Samurai 1, GH3, UPCA, GH4, P / I WHP, GH6, Super 2, GH13, P / F 51-93-C, Super 1, GH5, Mandau, GH7, and Talaga Bodas). The results showed that the addition of "ZincMicro" micro fertilizers to sorghum plants did not affect the intensity of anthracnose disease, however there were differences in the intensity of anthracnose diseases between sorghum genotypes. Numbu Genotype, GH 3, and GH 13 were relatively more resistant to anthracnose disease than the other genotypes.
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Mattiuzzi, Camilla, and Giuseppe Lippi. "Cancer statistics: a comparison between World Health Organization (WHO) and Global Burden of Disease (GBD)." European Journal of Public Health 30, no. 5 (November 25, 2019): 1026–27. http://dx.doi.org/10.1093/eurpub/ckz216.
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Abstract This study aims to define comparability of 2016 statistics between Global Health Estimates (GHE) and Global Health Data Exchange (GHDx) registries for disability-adjusted life years (DALYs) and mortality of the 25 most frequent worldwide malignancies. An excellent correlation can be found between the two registries for both cancer DALYs (r = 0.988) and mortality (r = 0.993). Cancer-related DALYs are substantially equivalent (mean bias, −1.9%; P = 0.603), while cancer mortality is modestly but significantly overestimated in GDHx (mean bias, 9.1%; P = 0.004). These results suggest that cancer DALYs estimate appear almost overlapping between GHE and GHDx registries, while cancer mortality is slightly overestimated in GDHx.
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Russo, Jacqueline, Marie-Rose Allo, Jean-Pierre Nenon, and Michel Brehélin. "The hemocytes of the mealybugs Phenacoccus manihoti and Planococcus citri (Insecta: Homoptera) and their role in capsule formation." Canadian Journal of Zoology 72, no. 2 (February 1, 1994): 252–58. http://dx.doi.org/10.1139/z94-034.
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Hemocytes of Phenacoccus manihoti and Planococcus citri were studied to determine general ultrastructure, phenoloxidase activity, and the presence or absence of a glycocalyx. Prohemocytes, oenocytoids, and granular hemocytes of types 1 (GH1), 2 (GH2), and 3 (GH3) were observed in P. manihoti. In P. citri we observed only GH2 and GH3 (macrophage-like cells). In addition to these hemocyte types, other cells that we believe to be fat-body cells were also observed free in the hemolymph. There was evidence of phenoloxidase activity in GH2 and GH3. The intensity of this reaction increased after parasitization of P. manihoti by the wasp Epidinocarsis lopezi. In most hemocyte types the glycocalyx was very little developed. In P. manihoti, lysis of hemocytes was observed in the vicinity of the parasitoid larva, leading to the formation of a capsule.
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Dai, Xin, Yan Tian, Jinting Li, Xiaoyun Su, Xuewei Wang, Shengguo Zhao, Li Liu, et al. "Metatranscriptomic Analyses of Plant Cell Wall Polysaccharide Degradation by Microorganisms in the Cow Rumen." Applied and Environmental Microbiology 81, no. 4 (December 12, 2014): 1375–86. http://dx.doi.org/10.1128/aem.03682-14.
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ABSTRACTThe bovine rumen represents a highly specialized bioreactor where plant cell wall polysaccharides (PCWPs) are efficiently deconstructed via numerous enzymes produced by resident microorganisms. Although a large number of fibrolytic genes from rumen microorganisms have been identified, it remains unclear how they are expressed in a coordinated manner to efficiently degrade PCWPs. In this study, we performed a metatranscriptomic analysis of the rumen microbiomes of adult Holstein cows fed a fiber diet and obtained a total of 1,107,083 high-quality non-rRNA reads with an average length of 483 nucleotides. Transcripts encoding glycoside hydrolases (GHs) and carbohydrate binding modules (CBMs) accounted for ∼1% and ∼0.1% of the total non-rRNAs, respectively. The majority (∼98%) of the putative cellulases belonged to four GH families (i.e., GH5, GH9, GH45, and GH48) and were primarily synthesized byRuminococcusandFibrobacter. Notably, transcripts for GH48 cellobiohydrolases were relatively abundant compared to the abundance of transcripts for other cellulases. Two-thirds of the putative hemicellulases were of the GH10, GH11, and GH26 types and were produced by members of the generaRuminococcus,Prevotella, andFibrobacter. Most (∼82%) predicted oligosaccharide-degrading enzymes were GH1, GH2, GH3, and GH43 proteins and were from a diverse group of microorganisms. Transcripts for CBM10 and dockerin, key components of the cellulosome, were also relatively abundant. Our results provide metatranscriptomic evidence in support of the notion that members of the generaRuminococcus,Fibrobacter, andPrevotellaare predominant PCWP degraders and point to the significant contribution of GH48 cellobiohydrolases and cellulosome-like structures to efficient PCWP degradation in the cow rumen.
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Graham, William. "HealthPRO Electronic Commerce Alliance with Global Healthcare Exchange (GHX); A GPO Strategic Alliance with an Open Exchange Accelerates Electronic Commerce Benefits." Healthcare Quarterly 6, no. 1 (December 15, 2002): 85–87. http://dx.doi.org/10.12927/hcq..16651.
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Sabater, Carlos, Lorena Ruiz, and Abelardo Margolles. "A Machine Learning Approach to Study Glycosidase Activities from Bifidobacterium." Microorganisms 9, no. 5 (May 11, 2021): 1034. http://dx.doi.org/10.3390/microorganisms9051034.
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This study aimed to recover metagenome-assembled genomes (MAGs) from human fecal samples to characterize the glycosidase profiles of Bifidobacterium species exposed to different prebiotic oligosaccharides (galacto-oligosaccharides, fructo-oligosaccharides and human milk oligosaccharides, HMOs) as well as high-fiber diets. A total of 1806 MAGs were recovered from 487 infant and adult metagenomes. Unsupervised and supervised classification of glycosidases codified in MAGs using machine-learning algorithms allowed establishing characteristic hydrolytic profiles for B. adolescentis, B. bifidum, B. breve, B. longum and B. pseudocatenulatum, yielding classification rates above 90%. Glycosidase families GH5 44, GH32, and GH110 were characteristic of B. bifidum. The presence or absence of GH1, GH2, GH5 and GH20 was characteristic of B. adolescentis, B. breve and B. pseudocatenulatum, while families GH1 and GH30 were relevant in MAGs from B. longum. These characteristic profiles allowed discriminating bifidobacteria regardless of prebiotic exposure. Correlation analysis of glycosidase activities suggests strong associations between glycosidase families comprising HMOs-degrading enzymes, which are often found in MAGs from the same species. Mathematical models here proposed may contribute to a better understanding of the carbohydrate metabolism of some common bifidobacteria species and could be extrapolated to other microorganisms of interest in future studies.
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Stewart, M. K., and B. D. Fahey. "Landuse effects on runoff generating processes in tussock grassland indicated by mean transit time estimation using tritium." Hydrology and Earth System Sciences Discussions 7, no. 1 (February 10, 2010): 1073–102. http://dx.doi.org/10.5194/hessd-7-1073-2010.
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Abstract. The east Otago uplands of New Zealand's South Island have long been studied because of the environmental consequences of converting native tussock grasslands to other land covers, notably forestry and pasture for stock grazing. Early studies showed that afforestation substantially reduced annual water yield, stream peak flows, and 7-day low flows, mainly as a consequence of increased interception. Tritium measurements have indicated that surprisingly old water is present in catchments GH1 and GH2, and the small headwater wetland and catchment (GH5). The old water contributes strongly to baseflow (and therefore also to quickflow). The data have been simulated assuming the presence of two types of water in the baseflow, young water from shallow aquifers connecting hillside regolith with the stream, and old water from deep bedrock aquifers, respectively. The mean transit time of the young water is of the order of months, while that of the old water is 25–26 years as revealed by the presence of tritium originating from the bomb-peak in NZ rainfall in late 1960s and early 1970s. Such a long transit time indicates slow release from groundwater reservoirs within the bedrock, which constitute by far the larger of the water stores. Comparison of the results from catchments GH1 (tussock) and GH2 (pine forest) suggests that about equal quantities of water (85 mm annually) are contributed from the deep aquifers in the two catchments, although runoff from the shallow aquifers has been strongly reduced by afforestation in GH2.
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Book, Adam J., Gina R. Lewin, Bradon R. McDonald, Taichi E. Takasuka, Drew T. Doering, Aaron S. Adams, Joshua A. V. Blodgett, et al. "Cellulolytic Streptomyces Strains Associated with Herbivorous Insects Share a Phylogenetically Linked Capacity To Degrade Lignocellulose." Applied and Environmental Microbiology 80, no. 15 (May 16, 2014): 4692–701. http://dx.doi.org/10.1128/aem.01133-14.
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ABSTRACTActinobacteria in the genusStreptomycesare critical players in microbial communities that decompose complex carbohydrates in the soil, and these bacteria have recently been implicated in the deconstruction of plant polysaccharides for some herbivorous insects. Despite the importance ofStreptomycesto carbon cycling, the extent of their plant biomass-degrading ability remains largely unknown. In this study, we compared four strains ofStreptomycesisolated from insect herbivores that attack pine trees: DpondAA-B6 (SDPB6) from the mountain pine beetle, SPB74 from the southern pine beetle, and SirexAA-E (SACTE) and SirexAA-G from the woodwasp,Sirex noctilio. Biochemical analysis of secreted enzymes demonstrated that only two of these strains, SACTE and SDPB6, were efficient at degrading plant biomass. Genomic analyses indicated that SACTE and SDPB6 are closely related and that they share similar compositions of carbohydrate-active enzymes. Genome-wide proteomic and transcriptomic analyses revealed that the major exocellulases (GH6 and GH48), lytic polysaccharide monooxygenases (AA10), and mannanases (GH5) were conserved and secreted by both organisms, while the secreted endocellulases (GH5 and GH9 versus GH9 and GH12) were from diverged enzyme families. Together, these data identify two phylogenetically related insect-associatedStreptomycesstrains with high biomass-degrading activity and characterize key enzymatic similarities and differences used by these organisms to deconstruct plant biomass.
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Cavalcante, Edilaine, Alex Ribeiro, Matheus do Nascimento, Analiza Silva, Crisieli Tomeleri, Hellen Nabuco, Fábio Pina, et al. "Effects of Different Resistance Training Frequencies on Fat in Overweight/Obese Older Women." International Journal of Sports Medicine 39, no. 07 (May 18, 2018): 527–34. http://dx.doi.org/10.1055/a-0599-6555.
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Abstract Aim This study compared the effect of different resistance training (RT) frequencies on total, android, gynoid and trunk body fat in overweight/obese older women. Methods Fifty-seven overweight/obese older women (66.9±5.3 years and 39.9±4.9% body fat) were randomly assigned to one of three groups: a group performing RT twice a week (G2X), a group performing RT three times a week (G3X), or a non-exercise control group (CG). Both training groups performed the same 12-week RT program consisting of 8 exercises that trained all major muscle groups. Dual-energy X-ray absorptiometry was used to assess body composition. Results After the intervention period, both G2X and G3X demonstrated significant (P<0.05) reductions in adiposity compared to the CG for total body fat (G2X=–1.7%, G3X=–2.7%, CG=+2.1%), android fat (G2X=–6.2%, G3X=–7.0%, CG=+8.6%), gynoid fat (G2X=–2.5%, G3X=–2.9%, CG=+1.0%), and trunk fat (G2X=–2.5%, G3X=–3.0%, CG=+2.9%), with no significant differences between training groups. Conclusion These results demonstrate that a low-volume 12-week RT program performed two or three times per week causes decreases in total and regional fat deposition with the greatest reductions occurring in the android region.
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Todorova, Nadezhda, Miroslav Rangelov, Vanya Bogoeva, Vishnya Stoyanova, Anna Yordanova, Ginka Nikolova, Hristo Georgiev, et al. "Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q." International Journal of Molecular Sciences 22, no. 15 (August 1, 2021): 8288. http://dx.doi.org/10.3390/ijms22158288.
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We addressed the issue of C1q autoantigenicity by studying the structural features of the autoepitopes recognized by the polyclonal anti-C1q antibodies present in Lupus Nephritis (LN) sera. We used six fractions of anti-C1q as antigens and selected anti-idiotypic scFv antibodies from the phage library “Griffin.1”. The monoclonal scFv A1 was the most potent inhibitor of the recognition of C1q and its fragments ghA, ghB and ghC, comprising the globular domain gC1q, by the lupus autoantibodies. It was sequenced and in silico folded by molecular dynamics into a 3D structure. The generated 3D model of A1 elucidated CDR similarity to the apical region of gC1q, thus mapping indirectly for the first time a globular autoepitope of C1q. The VH CDR2 of A1 mimicked the ghA sequence GSEAD suggested as a cross-epitope between anti-DNA and anti-C1q antibodies. Other potential inhibitors of the recognition of C1q by the LN autoantibodies among the selected recombinant antibodies were the monoclonal scFv F6, F9 and A12.
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Stewart, M. K., and B. D. Fahey. "Runoff generating processes in adjacent tussock grassland and pine plantation catchments as indicated by mean transit time estimation using tritium." Hydrology and Earth System Sciences 14, no. 6 (June 24, 2010): 1021–32. http://dx.doi.org/10.5194/hess-14-1021-2010.
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Abstract. The east Otago uplands of New Zealand's South Island have long been studied because of the environmental consequences of converting native tussock grasslands to other land covers, notably forestry and pasture for stock grazing. Early studies showed that afforestation substantially reduced annual water yield, stream peak flows, and 7-day low flows, mainly as a consequence of increased interception. Tritium measurements have indicated that surprisingly old water is present in catchments GH1 and GH2, and the small headwater wetland and catchment (GH5), and contributes strongly to baseflow. The data have been simulated assuming the presence of two types of water in the baseflow, young water from shallow aquifers connecting hillside regolith with the stream, and old water from deep bedrock aquifers, respectively. The mean transit time of the young water is approximately one month, while that of the old water is 25–26 years as revealed by the presence of tritium originating from the bomb-peak in NZ rainfall in late 1960s and early 1970s. Such a long transit time indicates slow release from groundwater reservoirs within the bedrock, which constitute by far the larger of the water stores. Comparison of the results from catchments GH1 (tussock) and GH2 (pine forest) suggests that about equal quantities of water (85 mm/a) are contributed from the deep aquifers in the two catchments, although runoff from the shallow aquifers has been strongly reduced by afforestation in GH2. This study has revealed the presence of a long transit time component of water in runoff in a catchment with crystalline metamorphic bedrock.
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Li, Xiaoqing, Iris Vogeler, and Luitgard Schwendenmann. "Soil aggregation and soil fraction associated carbon under different vegetation types in a complex landscape." Soil Research 57, no. 3 (2019): 215. http://dx.doi.org/10.1071/sr18193.
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Land cover change has been shown to affect soil characteristics and soil organic carbon (SOC) storage. However, little is known about the driving factors associated with soil carbon (C) stabilisation in complex landscapes. This study was conducted to investigate the effects of both inherent soil characteristics and recent vegetation cover change on soil aggregation and soil fraction associated C in a complex landscape. The specific objectives were: (1) determine bulk soil properties and SOC saturation deficit; (2) quantify soil aggregation, soil size-density fractions, and soil fraction associated C; and (3) identify the factors that influence soil fractions and soil fraction associated C in two adjacent catchments differing in vegetation cover, Central Otago, New Zealand. Catchment GH1 (n = 17 plots) was dominated by tussock grassland and native shrubs. Catchment GH2 (n = 21 plots) was converted from tussock grassland into a pine forest in 1981. The catchments differed in soil texture (e.g. sand content GH1: 62.9%, GH2: 50.7%, P = 0.007), soil SOC stocks (GH1: 5.0 kg C m−2, GH2: 4.3 kg C m−2, P = 0.04), mean weight diameter (MWD) (GH1: 782.3 µm, GH2: 736.5 µm, P = 0.002), and proportion of the macroaggregate heavy fraction (macro_HF) (GH1: 72%, GH2: 55%, P = 0.01). No significant differences were found in SOC saturation deficit (GH1: 14.8 mg g−1, GH2: 13.1 mg g−1, P = 0.13). Dominant vegetation cover explained 21% of the variation in MWD in GH1, whereas silt+clay C content explained 31.6% of the MWD variation in GH2. The macro_HF fraction was negatively correlated with the proportion of silt+clay. Our findings illustrate that physical and chemical soil characteristics are important drivers in such a complex landscape and may have masked the effect of recent vegetation change on soil aggregation, and soil fraction associated C.
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Zou, C., J. Meng, Z. Li, M. Wei, J. Song, B. Chen, and B. Wei. "First Report of Yam mild mosaic virus in Yam in Guangxi Province, China." Plant Disease 95, no. 10 (October 2011): 1320. http://dx.doi.org/10.1094/pdis-04-11-0350.
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Yams (Dioscorea spp.) are widely grown in China as vegetables and herbal medicine. However, studies on viral diseases on yams are still limited. As a pilot project of a government initiative for improving yam productivity, a small study was conducted in Guangxi, a southern province of China, on viral disease in yams. Incidence of virus-like disease for the three extensively grown D. alata cultivars, GH2, GH5, and GH6, were 12 to 40%, 12 to 29%, and 11 to 25%, respectively, as found in a field survey with a five-plot sampling method in 2010. A total of 112 leaf samples showing mosaic or mottling or leaves without symptoms were collected from the cvs. GH2, GH5, GH6, and seven additional cultivars (D. alata cvs. GY2, GY23, GY47, GY69, GY62, GY72, and D. batatas cv. Tiegun). To determine if the symptoms were caused by Yam mild mosaic virus (YMMV; genus Potyvirus, family Potyviridae), total RNA was extracted from leaves with a commercial RNA purification kit (TIANGEN, Beijing, China), and reverse-transcription (RT)-PCR was conducted with a YMMV-specific primer pair (4) that amplifies the 3′-terminal portion of the viral genome. A PCR product with the predicted size of 262 bp was obtained from samples of GH5 (number testing positive of total number of leaves = 5 of 12), GH6 (24 of 42), and GY72 (1 of 1), but not from asymptomatic leaves. PCR products from a GH5 sample (YMMV-Nanning) and a GH6 sample (YMMV-Luzhai) were cloned and sequenced using an ABI PRISM 3770 DNA Sequencer. The two PCR products were 97% identical at nucleotide (nt) level and with the highest homology (89% identity) to a YMMV isolate (GenBank Accession No. AJ305466). To further characterize the isolates, degenerate primers (2) were used to amplify viral genome sequence corresponding to the C-terminal region of the nuclear inclusion protein b (NIb) and the N-terminal region of the coat protein (CP). These 781-nt fragments were sequenced and a new primer, YMMV For1 (5′-TTCATGTCGCACAAAGCAGTTAAG-3′) corresponding to the NIb region, was designed and used together with primer YMMV UTR 1R to amplify a fragment that covers the complete CP region of YMMV by RT-PCR. These 1,278-nt fragments were sequenced (GenBank Accession Nos. JF357962 and JF357963). CP nucleotide sequences of the YMMV-Nanning and YMMV-Luzhai isolates were 94% similar, while amino acid sequences were 99% similar. BLAST searches revealed a nucleotide identity of 82 to 89% and a similarity of 88 to 97% for amino acids to sequences of YMMV isolates (AF548499 and AF548519 and AAQ12304 and BAA82070, respectively) in GenBank. YMMV is known to be prevalent on D. alata in Africa and the South Pacific, and has recently been identified in the Caribbean (1) and Colombia (3). To our knowledge, this is the first report of the natural occurrence of YMMV in China and it may have implications for yam production and germplasm exchange within China. References: (1) M. Bousalem and S. Dallot. Plant Dis. 84:200, 2000. (2) D. Colinet et al. Phytopathology 84:65, 1994. (3) S. Dallot et al. Plant Dis. 85:803, 2001. (4) R. A. Mumford and S. E. Seal. J. Virol. Methods 69:73, 1997.
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Dettori, Maria Luisa, Michele Pazzola, Emanuela Pira, Pietro Paschino, and Giuseppe Massimo Vacca. "The sheep growth hormone gene polymorphism and its effects on milk traits." Journal of Dairy Research 82, no. 2 (February 11, 2015): 169–76. http://dx.doi.org/10.1017/s0022029915000047.
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Growth hormone (GH) is encoded by the GH gene, which may be single copy or duplicate in sheep. The two copies of the sheep GH gene (GH1/GH2-N and GH2-Z) were entirely sequenced in one 106 ewes of Sarda breed, in order to highlight sequence polymorphisms and investigate possible association between genetic variants and milk traits. Milk traits included milk yield, fat, protein, casein and lactose percentage. We evidenced 75 nucleotide changes. Transcription factor binding site prediction revealed two sequences potentially recognised by the pituitary-specific transcription factor POU1FI at the GH1/GH2-N gene, which were lost at the promoter of GH2-Z, which might explain the different tissues of expression of GH1/GH2-N (pituitary) and GH2-Z (placenta). Significant differences in milk traits were observed among genotypes at polymorphic loci only for the GH2-Z gene. Sheep with homozygote genotype ss748770547 CC had higher fat percentage (P < 0·01) than TT. SNP ss748770547 was part of a potential transcription factor binding site for C/EBP alpha (CCAAT/Enhancer Binding Protein), which is involved in the regulation of adipogenesis and adipoblast differentiation. SNP ss748770547, located in the GH2-Z gene 5′ flanking region, may be a causal mutation affecting milk fat content. These findings might contribute to the knowledge of the sheep GH locus and might be useful in selection processes in sheep.
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Wyse, B., M. Waters, and C. Sernia. "Stimulation of the renin-angiotensin system by growth hormone in Lewis dwarf rats." American Journal of Physiology-Endocrinology and Metabolism 265, no. 2 (August 1, 1993): E332—E339. http://dx.doi.org/10.1152/ajpendo.1993.265.2.e332.
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A genetically growth hormone (GH)-deficient strain of Lewis rats was used to test the hypothesis that the actions of GH on electrolyte and fluid homeostasis are mediated by the renin-angiotensin-aldosterone system (RAAS). Dwarf rats injected with recombinant bGH (2 mg.kg-1 x day-1) for 7 days (group GH1+) and 28 days (group GH4+), respectively, were compared with saline-injected dwarf (group GH-) and normal (group N) Lewis rats. GH decreased Na+ excretion and increased renal glomerular filtration rate in dwarf rats. The dietary intake and plasma concentrations of Na+ and K+ remained unchanged. GH increased plasma insulin-like growth factor I (IGF-I) concentrations in dwarf rats (GH - = 109 +/- 9, GH1+ = 184 +/- 5, GH4+ = 189 +/- 28, N = 477 +/- 29 ng/ml plasma). Plasma angiotensinogen increased towards the levels found in normal Lewis rats (GH- = 859 +/- 38, GH1+ = 906 +/- 18, GH4+ = 1,027 +/- 19, N = 1497 +/- 80 ng angiotensin I/ml plasma); plasma renin activity increased above that of the normal Lewis (GH- = 10.2 +/- 0.6, GH1+ = 11.7 +/- 0.7, GH4+ = 16.7 +/- 2.4, N = 10.6 +/- 0.8 ng angiotensin I.ml plasma-1 x h-1). Plasma aldosterone, corticosterone, and triodothyronine concentrations were unchanged by GH treatment. Angiotensin II receptor densities in GH- rats (liver = 356 +/- 23, kidney = 228 +/- 28, adrenal = 478 +/- 58 fmol/mg protein) were upregulated by GH (GH4+ rats; liver = 573 +/- 27, kidney = 360 +/- 86, adrenal = 721 +/- 78 fmol/mg protein).(ABSTRACT TRUNCATED AT 250 WORDS)
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Kyu, May Thin, Shunsuke Nishio, Koki Noda, Bay Dar, San San Aye, and Tsukasa Matsuda. "Predominant secretion of cellobiohydrolases and endo-β-1,4-glucanases in nutrient-limited medium by Aspergillus spp. isolated from subtropical field." Journal of Biochemistry 168, no. 3 (July 29, 2020): 243–56. http://dx.doi.org/10.1093/jb/mvaa049.
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Abstract Biological degradation of cellulose from dead plants in nature and plant biomass from agricultural and food-industry waste is important for sustainable carbon recirculation. This study aimed at searching diverse cellulose-degrading systems of wild filamentous fungi and obtaining fungal lines useful for cellooligosaccharide production from agro-industrial wastes. Fungal lines with cellulolytic activity were screened and isolated from stacked rice straw and soil in subtropical fields. Among 13 isolated lines, in liquid culture with a nutrition-limited cellulose-containing medium, four lines of Aspergillus spp. secreted 50–60 kDa proteins as markedly dominant components and gave clear activity bands of possible endo-β-1,4-glucanase in zymography. Mass spectroscopy (MS) analysis of the dominant components identified three endo-β-1,4-glucanases (GH5, GH7 and GH12) and two cellobiohydrolases (GH6 and GH7). Cellulose degradation by the secreted proteins was analysed by LC-MS-based measurement of derivatized reducing sugars. The enzymes from the four Aspergillus spp. produced cellobiose from crystalline cellulose and cellotriose at a low level compared with cellobiose. Moreover, though smaller than that from crystalline cellulose, the enzymes of two representative lines degraded powdered rice straw and produced cellobiose. These fungal lines and enzymes would be effective for production of cellooligosaccharides as cellulose degradation-intermediates with added value other than glucose.
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Murray, Robert D., Judith E. Adams, and Stephen M. Shalet. "A Densitometric and Morphometric Analysis of the Skeleton in Adults with Varying Degrees of Growth Hormone Deficiency." Journal of Clinical Endocrinology & Metabolism 91, no. 2 (February 1, 2006): 432–38. http://dx.doi.org/10.1210/jc.2005-0897.
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Context: Low bone mass is a characteristic feature of the adult GH deficiency (GHD) syndrome, but recent dual-energy x-ray absorptiometry (DXA) studies in patients with GH-receptor and GHRH-receptor gene mutations suggest that the situation is more complex. Objective: The objective was to define bone areal and volumetric densities and morphometry in hypopituitary adults. Design: The study was a cross-sectional case-controlled study performed between 1999 and 2001. Setting: The study was undertaken at an endocrine tertiary referral center. Patients: Thirty patients with GHD, 24 with GH insufficiency (GHI) [peak GH, 3–7 μg/liter (9–21 mU/liter)], and 30 age- and sex-matched controls were included for study. Main Outcome Measures: DXA and peripheral quantitative computed tomography (pQCT) derived bone density and morphometry were measured. Results: No densitometric or morphometric abnormalities were detected in GHD patients who acquired their deficiency during adult life. GHD adults of childhood-onset (CO-GHD) showed decreased bone mineral density at the lumbar spine and hip on DXA. pQCT of the radius showed that CO-GHD patients have normal trabecular bone mineral density and only a 2% decrease in cortical density. Radial bone area was reduced 14.5%, cortical thickness 20%, and cortical cross-sectional area 23%, culminating in a reduction in cortical bone of 25%. The “apparent” low DXA bone density in CO-GHD adults therefore relates primarily to reduced cortical thickness and smaller bone area. DXA and pQCT data derived from adults with GHI revealed no evidence of densitometric or morphometric abnormalities. Conclusions: 1) Adult-onset GHD patients have normal bone density and size. 2) CO-GHD adults have marginally reduced cortical density but significantly reduced cortical bone as a result of reduced cortical thickness and bone size. 3) GHI has no measurable impact on the skeleton.
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Ribeiro, Alex S., Matheus A. Nascimento, Brad J. Schoenfeld, João Pedro Nunes, Andreo F. Aguiar, Edilaine F. Cavalcante, Analiza M. Silva, Luís B. Sardinha, Steven J. Fleck, and Edilson S. Cyrino. "Effects of Single Set Resistance Training With Different Frequencies on a Cellular Health Indicator in Older Women." Journal of Aging and Physical Activity 26, no. 4 (October 1, 2018): 537–43. http://dx.doi.org/10.1123/japa.2017-0258.
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The main purpose of this study was to compare the effects of resistance training (RT) performed two versus three times per week on phase angle (a cellular health indicator) in older women. A total of 39 women (69.1 ± 5.5 years) were randomly assigned to perform a RT program two (G2X) or three (G3X) days per week for 12 weeks. The RT was a whole-body program (eight exercises, one set, 10–15 repetitions). Phase angle, resistance, reactance, and total body water were assessed by bioimpedance spectroscopy. Intracellular water, reactance, and phase angle increased significantly in G2X (2.1%, 3.0%, and 5.6%, respectively) and G3X (5.0%, 6.9%, and 10.3%, respectively) from pretraining to posttraining, with no significant difference between groups. Bioimpedance resistance decreased similarly in both groups (G2X = −1.7% vs. G3X = −3.2%). We conclude that a single set RT program with a frequency of 2 days per week may be sufficient to promote an improvement in cellular health in older women.
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de Giuseppe, Priscila Oliveira, Tatiana de Arruda Campos Brasil Souza, Flavio Henrique Moreira Souza, Leticia Maria Zanphorlin, Carla Botelho Machado, Richard John Ward, Joao Atilio Jorge, Rosa dos Prazeres Melo Furriel, and Mario Tyago Murakami. "Structural basis for glucose tolerance in GH1 β-glucosidases." Acta Crystallographica Section D Biological Crystallography 70, no. 6 (May 24, 2014): 1631–39. http://dx.doi.org/10.1107/s1399004714006920.
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Product inhibition of β-glucosidases (BGs) by glucose is considered to be a limiting step in enzymatic technologies for plant-biomass saccharification. Remarkably, some β-glucosidases belonging to the GH1 family exhibit unusual properties, being tolerant to, or even stimulated by, high glucose concentrations. However, the structural basis for the glucose tolerance and stimulation of BGs is still elusive. To address this issue, the first crystal structure of a fungal β-glucosidase stimulated by glucose was solved in native and glucose-complexed forms, revealing that the shape and electrostatic properties of the entrance to the active site, including the +2 subsite, determine glucose tolerance. The aromatic Trp168 and the aliphatic Leu173 are conserved in glucose-tolerant GH1 enzymes and contribute to relieving enzyme inhibition by imposing constraints at the +2 subsite that limit the access of glucose to the −1 subsite. The GH1 family β-glucosidases are tenfold to 1000-fold more glucose tolerant than GH3 BGs, and comparative structural analysis shows a clear correlation between active-site accessibility and glucose tolerance. The active site of GH1 BGs is located in a deep and narrow cavity, which is in contrast to the shallow pocket in the GH3 family BGs. These findings shed light on the molecular basis for glucose tolerance and indicate that GH1 BGs are more suitable than GH3 BGs for biotechnological applications involving plant cell-wall saccharification.
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Madeira, João L. O., Alexander A. L. Jorge, Regina M. Martin, Luciana R. Montenegro, Marcela M. Franca, Everlayny F. Costalonga, Fernanda A. Correa, et al. "A homozygous point mutation in the GH1 promoter (c.-223C>T) leads to reduced GH1 expression in siblings with isolated GH deficiency (IGHD)." European Journal of Endocrinology 175, no. 2 (August 2016): K7—K15. http://dx.doi.org/10.1530/eje-15-0149.
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Context Mutations in the GH1 promoter are a rare cause of isolated growth hormone deficiency (IGHD). Objective To identify the molecular aetiology of a family with IGHD. Design DNA sequencing, electromobility shift (EMSA) and luciferase reporter assays. Setting University Hospital. Patients Three siblings (2M) born to consanguineous parents presented with IGHD with normal pituitary on MRI. Methods The GH1 proximal promoter, locus control region, five exons and four introns as well as GHRHR gene were sequenced in genomic DNA by Sanger method. DNA–protein interaction was evaluated by EMSA in nuclear extracts of GH3 pituitary cells. Dual-luciferase reporter assays were performed in cells transiently transfected with plasmids containing four different combinations of GH1 allelic variants (AV). Results The patients harboured two homozygous variants (c.-185T>C and c.-223C>T) in the GH1 promoter within a highly conserved region and predicted binding sites for POU1F1/SP1 and SP1 respectively. The parents and brother were carriers and these variants were absent in 100 controls. EMSA demonstrated absent binding of GH3 nuclear extract to the c.-223C>T variant and normal binding of both POU1F1 protein and GH3 nuclear extract to the c.-185T>C variant. In contrast to GH1 promoter with AV only at c.-185, the GH1 promoter containing the AV only at c.-223 and at both positions drove significantly less expression of luciferase compared with the promoter containing either positions wild type in luciferase reporter assays. Conclusion To our knowledge, c.-223C>T is the first homozygous point mutation in the GH1 promoter that leads to short stature due to IGHD.
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Murray, Robert D., Martin Bidlingmaier, Christian J. Strasburger, and Stephen M. Shalet. "The Diagnosis of Partial Growth Hormone Deficiency in Adults with a Putative Insult to the Hypothalamo-Pituitary Axis." Journal of Clinical Endocrinology & Metabolism 92, no. 5 (May 1, 2007): 1705–9. http://dx.doi.org/10.1210/jc.2006-2055.
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Abstract Context: Similar to patients with severe GH deficiency (GHD), those with a more moderate impairment of GH secretion [GH insufficiency (GHI)] have abnormal body composition, dyslipidemia, and insulin resistance. Given the inherent problems in the diagnosis of severe GHD, the situation is likely to be even more difficult in individuals with GHI. Objective: The objective of the study was to examine the utility of GH stimulation tests and GH-dependent proteins in the diagnosis of GHI. Design: The study was a cross-sectional, case-controlled study. Patients: The study included 31 patients with GHD, 23 with GHI [peak GH 3–7 μg/liter (9–21 mU/liter)], and 30 age- and sex-matched controls. Main Outcome Measures: Demographic and biochemical markers of GH status were measured. Results: Nineteen of the patients with GHI (83%) had no additional anterior pituitary hormone deficits. Ten GHI patients showed discordant GH status based on the two GH stimulation tests performed. GH status was defined by the highest peak GH value achieved; in four this was to the insulin tolerance test (ITT), four the arginine test, and two the GHRH-arginine test. In five of the six patients in whom GH status was not defined by the ITT, peak GH levels to the ITT were in the range 2.4–2.9 μg/liter. IGF-I values for the GHI adults were significantly lower than the control subjects (121 ± 48 vs. 162 ± 75 μg/liter; P < 0.05); however, only six (26%) had values below the 10th percentile of levels seen in the control group. IGF binding protein-3 and acid labile subunit levels of the GHI adults were not significantly different from the controls. Conclusion: The diagnosis of GHI in an individual is extremely difficult because the patients rarely exhibit additional pituitary hormone deficits, and levels of GH-dependent proteins are normal in the majority. Diagnosis relies heavily on GH stimulation tests and requires two tests in all patients to define GHI; obesity when present is potentially a major confounder.
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Kmieć, M., A. Terman, H. Wierzbicki, and S. Zych. "Association of GH Gene Polymorphism with Semen Parameters of Boars." Acta Veterinaria Brno 76, no. 1 (2007): 41–46. http://dx.doi.org/10.2754/avb200776010041.
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Relations between polymorphism of the Growth Hormone gene and semen characters were analyzed. The DNA for the purpose of examination was isolated from the peripheral blood of 173 boars. In the boar herd under study the frequency of allele occurrence for the GH/MspI was as follows: allele GHA - 0.79 and allele GHB - 0.21. As far as the GH/HaeII polymorphism is concerned, the relevant frequency was as follows: allele GHA - 0.53 and allele GHB - 0.47, respectively. The relationship between the GH genotypes and semen characteristic traits were analyzed. The study showed that boars with GHBGHB genotype of both polymorphous loci of the GH gene produced ejaculates of larger volume, higher percentage, number of normozosperms in the ejaculate and number of insemination as compared to GHA GHA and GHAGHB boars. Our current findings suggested that polymorphism of the GH/MspI and GH/HaeII might have potential effect for reproductive performance traits of boars.
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Jena, N. R., Vivek Gaur, and P. C. Mishra. "The R- and S-diastereoisomeric effects on the guanidinohydantoin-induced mutations in DNA." Physical Chemistry Chemical Physics 17, no. 27 (2015): 18111–20. http://dx.doi.org/10.1039/c5cp02636a.
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McKay, S. J., J. Trautner, M. J. Smith, B. F. Koop, and R. H. Devlin. "Evolution of duplicated growth hormone genes in autotetraploid salmonid fishes." Genome 47, no. 4 (August 1, 2004): 714–23. http://dx.doi.org/10.1139/g04-018.
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A defining character of the piscine family Salmonidae is autotetraploidy resulting from a genome-doubling event some 25–100 million years ago. Initially, duplicated genes may have undergone concerted evolution and tetrasomic inheritance. Homeologous chromosomes eventually diverged and the resulting reduction in recombination and gene conversion between paralogous genes allowed the re-establishment of disomic inheritance. Among extant salmonine fishes (e.g. salmon, trout, char) the growth hormone (GH) gene is generally represented by two functional paralogs, GH1 and GH2. Sequence analyses of salmonid GH genes from species of subfamilies Coregoninae (whitefish, ciscos) and Salmoninae were used to examine the evolutionary history of the duplicated GH genes. Two divergent GH gene paralogs were also identified in Coregoninae, but they were not assignable to the GH1 and GH2 categories. The average sequence divergence between the coregonine GH genes was more than twofold lower than the corresponding divergence between the salmonine GH1 and GH2. Phylogenetic analysis of the coregonine GH paralogs did not resolve their relationship to the salmonine paralogs. These findings suggest that disomic inheritance of two GH genes was established by different mechanisms in these two subfamilies.Key words: salmon, phylogeny, growth hormone, disomy, tetraploidy.
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Franco, Maurício Machaim, Bárbara Amélia Aparecida Santana, Luiz Ricardo Goulart Filho, Robson Carlos Antunes, and Maurício Borges. "DETERMINAÇÃO DE POLIMORFISMOS NO GENE DO HORMÔNIO DO CRESCIMENTO EM TRÊS POPULAÇÕES DE SUÍNOS." Ciência Rural 31, no. 2 (April 2001): 329–31. http://dx.doi.org/10.1590/s0103-84782001000200023.
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Dois polimorfismos (GHC e GHD) no gene que codifica para o hormônio do crescimento foram determinados em um total de 96 animais de três raças de suínos (Pietrain, Large White e Landrace), através da PCR-RFLP. As freqüências alélicas observadas para GHC foram C1 0,42, C2 0,0, C3 0,06 e C4 0,52 para Landrace; C1 0,0, C2 0,03, C3 0,14 e C4 0,83 para Large White e C1 0,02, C2 0,25, C3 0,28 e C4 0,45 para Pietrain. Para GHD, as freqüências alélicas observadas foram D1 0,69 e D2 0,31 para Landrace; D1 0,25 e D2 0,75 para Large White e D1 0,72 e D2 0,28 para Pietrain.
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Alam, Shanon Iffat, Hadeel Hammoda, Faiza Khan, Reem Al Enazi, and Ipek Goktepe. "Electrical Conductivity, pH, Organic Matter and Texture of Selected Soils Around the Qatar University Campus." Research in Agriculture Livestock and Fisheries 7, no. 3 (December 31, 2020): 403–9. http://dx.doi.org/10.3329/ralf.v7i3.51359.
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Assessment of soil quality by soil analysis is a valuable tool for a farm as it determines the inputs required for efficient and economical production. A proper soil test helps ensure the application of sufficient fertilizers to meet the requirements of the crop while taking advantage of the nutrients and conditions already present in the soil. Though soil pH, electrical conductivity (EC), organic matter and texture are important features that determine the fertility of the soil, quantitative information on these properties are limited. The objective of the present study was to assess the pH, EC, organic matters and texture of four selected locations around the Qatar University campus including Biology Field (BF), Science Garden (SG), Greenhouse Inside (GHI) and Greenhouse Outside (GHO). We observed significant differences in the pH, EC and organic matter contents among the four locations. The highest pH was observed in SG and lowest in GHI whereas highest EC was observed in GHI and lowest in BF. Highest organic matter was found in GHI and lowest in BF. The soil texture of the BF and SG was sandy clay while that of GHI and GHO was loamy sand. Considering all four parameters analyzed in the present study, the GHI soil was found more suitable for plant growth.
Res. Agric., Livest. Fish.7(3): 403-409, December 2020
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Li, Juan, Otor Al-Khalili, Semra Ramosevac, Douglas C. Eaton, and Donald D. Denson. "Protein-protein interaction between cPLA2 and splice variants of α-subunit of BK channels." American Journal of Physiology-Cell Physiology 298, no. 2 (February 2010): C251—C262. http://dx.doi.org/10.1152/ajpcell.00221.2009.
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Altering the splice variant composition of large-conductance Ca2+-activated potassium (BK) channels can alter their activity and apparent sensitivity to Ca2+ and other regulators of activity. We hypothesized that differences in the responsiveness to arachidonic acid of GH3 and GH4 cells was due to a difference in two splice variants, one present in GH3 cells and the other in GH4 cells. The sequences of the two splice variants differ from one another in several ways, but the largest difference is the presence or absence of 27 amino acids in the COOH terminus of the BK α-subunit. Open probability of the variant containing the 27 amino acids is significantly increased by arachidonic acid, while the variant lacking the 27 amino acids is insensitive to arachidonic acid. In addition, sensitivity of BK channels to arachidonic acid depends on cytosolic phospholipase A2 (cPLA2). Here we used the Mammalian Matchmaker two-hybrid assay and two BK α-subunit constructs with [rSlo(27)] and without [rSlo(0)] the 27-amino acid motif to determine whether cPLA2 associates with one construct [rSlo(27)] and not the other. We hypothesized that differential association of cPLA2 might explain the differing responsiveness of the two constructs and GH3 and GH4 cells to arachidonic acid. We found that cPLA2 is strongly associated with the COOH terminus of rSlo(27) and only very weakly associated with rSlo(0). We also found that arachidonic acid has a lower affinity for rSlo(0) than for rSlo(27). We conclude that the lack of response of BK channels in GH4 cells to arachidonic acid can be explained, in part, by the poor binding of cPLA2 to the COOH terminus of the rSlo(0) α-subunit, which is very similar to the splice variant found in the arachidonic acid-insensitive GH4 cells.
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Gold, Nicholas D., and Vincent J. J. Martin. "Global View of the Clostridium thermocellum Cellulosome Revealed by Quantitative Proteomic Analysis." Journal of Bacteriology 189, no. 19 (July 20, 2007): 6787–95. http://dx.doi.org/10.1128/jb.00882-07.
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ABSTRACT A metabolic isotope-labeling strategy was used in conjunction with nano-liquid chromatography-electrospray ionization mass spectrometry peptide sequencing to assess quantitative alterations in the expression patterns of subunits within cellulosomes of the cellulolytic bacterium Clostridium thermocellum, grown on either cellulose or cellobiose. In total, 41 cellulosomal proteins were detected, including 36 type I dockerin-containing proteins, which count among them all but three of the known docking components and 16 new subunits. All differential expression data were normalized to the scaffoldin CipA such that protein per cellulosome was compared for growth between the two substrates. Proteins that exhibited higher expression in cellulosomes from cellulose-grown cells than in cellobiose-grown cells were the cell surface anchor protein OlpB, exoglucanases CelS and CelK, and the glycoside hydrolase family 9 (GH9) endoglucanase CelJ. Conversely, lower expression in cellulosomes from cells grown on cellulose than on cellobiose was observed for the GH8 endoglucanase CelA; GH5 endoglucanases CelB, CelE, CelG; and hemicellulases XynA, XynC, XynZ, and XghA. GH9 cellulases were the most abundant group of enzymes per CipA when cells were grown on cellulose, while hemicellulases were the most abundant group on cellobiose. The results support the existing theory that expression of scaffoldin-related proteins is coordinately regulated by a catabolite repression type of mechanism, as well as the prior observation that xylanase expression is subject to a growth rate-independent type of regulation. However, concerning transcriptional control of cellulases, which had also been previously shown to be subject to catabolite repression, a novel distinction was observed with respect to endoglucanases.
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Suen, C. S., and W. W. Chin. "Ligand-dependent, Pit-1/growth hormone factor-1 (GHF-1)-independent transcriptional stimulation of rat growth hormone gene expression by thyroid hormone receptors in vitro." Molecular and Cellular Biology 13, no. 3 (March 1993): 1719–27. http://dx.doi.org/10.1128/mcb.13.3.1719.
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The expression of the rat growth hormone (rGH) gene in the anterior pituitary gland is modulated by Pit-1/GHF-1, a pituitary-specific transcription factor, and by other more widely distributed factors, such as the thyroid hormone receptors (TRs), Sp1, and the glucocorticoid receptor. Thyroid hormone (T3)-mediated transcriptional stimulation of rGH gene expression has been extensively studied in vivo and in vitro including the measurements of (i) rGH mRNA by blot hybridization, (ii) transcriptional rate of rGH gene by nuclear run-on, and (iii) reporter gene expression in which a chimeric plasmid containing 5'-flanking sequences of the rGH gene linked to a reporter gene has been transfected either stably or transiently into pituitary and/or nonpituitary cells. From these studies, it has been suggested that the Pit-1/GHF-1 binding site is necessary for full T3 action. We developed a cell-free in vitro transcription system to examine further the roles of the TRs and Pit-1/GHF-1 in rGH gene activation. Using GH3 nuclear extract as a source of TRs and Pit-1/GHF-1, this in vitro transcription assay showed that T3 stimulation of rGH promoter activity is dependent on the addition of T3 to the GH3 nuclear extract. This transcriptional stimulation was augmented with increasing concentrations of ligand and was T3, but not T4 or reverse T3, specific. T3-mediated stimulation of rGH promoter activity was completely abolished by preincubation of the nuclear extract with rGH-thyroid hormone response element (-200 to -160) but not with Pit-1/GHF-1 (-137 to -65) oligonucleotides. Further, neither deletion of both Pit-1/GHF-1 binding sites nor mutation of the proximal Pit-1/GHF-1 binding site from the rGH promoter abrogated the T3 effect. These results provide evidence that T3-stimulated rGH promoter activity is independent of Pit-1/GHF-1 and raise the possibility that the stimulation of rGH gene expression by T3 might involve direct interaction of TRs with the general transcriptional apparatus.
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Suen, C. S., and W. W. Chin. "Ligand-dependent, Pit-1/growth hormone factor-1 (GHF-1)-independent transcriptional stimulation of rat growth hormone gene expression by thyroid hormone receptors in vitro." Molecular and Cellular Biology 13, no. 3 (March 1993): 1719–27. http://dx.doi.org/10.1128/mcb.13.3.1719-1727.1993.
Full textAbstract:
The expression of the rat growth hormone (rGH) gene in the anterior pituitary gland is modulated by Pit-1/GHF-1, a pituitary-specific transcription factor, and by other more widely distributed factors, such as the thyroid hormone receptors (TRs), Sp1, and the glucocorticoid receptor. Thyroid hormone (T3)-mediated transcriptional stimulation of rGH gene expression has been extensively studied in vivo and in vitro including the measurements of (i) rGH mRNA by blot hybridization, (ii) transcriptional rate of rGH gene by nuclear run-on, and (iii) reporter gene expression in which a chimeric plasmid containing 5'-flanking sequences of the rGH gene linked to a reporter gene has been transfected either stably or transiently into pituitary and/or nonpituitary cells. From these studies, it has been suggested that the Pit-1/GHF-1 binding site is necessary for full T3 action. We developed a cell-free in vitro transcription system to examine further the roles of the TRs and Pit-1/GHF-1 in rGH gene activation. Using GH3 nuclear extract as a source of TRs and Pit-1/GHF-1, this in vitro transcription assay showed that T3 stimulation of rGH promoter activity is dependent on the addition of T3 to the GH3 nuclear extract. This transcriptional stimulation was augmented with increasing concentrations of ligand and was T3, but not T4 or reverse T3, specific. T3-mediated stimulation of rGH promoter activity was completely abolished by preincubation of the nuclear extract with rGH-thyroid hormone response element (-200 to -160) but not with Pit-1/GHF-1 (-137 to -65) oligonucleotides. Further, neither deletion of both Pit-1/GHF-1 binding sites nor mutation of the proximal Pit-1/GHF-1 binding site from the rGH promoter abrogated the T3 effect. These results provide evidence that T3-stimulated rGH promoter activity is independent of Pit-1/GHF-1 and raise the possibility that the stimulation of rGH gene expression by T3 might involve direct interaction of TRs with the general transcriptional apparatus.
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Desai, M. P., P. Colaco, C. Choksi, M. Ambadkar, F. E. Vaz, R. Mehta, C. Cupte, and B. J. Wadia. "GROWTH HORMONE DEFICIENCY (GHD) AND GROWTH HORMONE RESISTANCE (GHR) IN INDIAN CHILDREN." Pediatric Research 33 (May 1993): S33. http://dx.doi.org/10.1203/00006450-199305001-00182.
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Ioimo, Irene, Carmen Guarracino, Cristina Meazza, Horacio M. Domené, and Mauro Bozzola. "Same Phenotype in Children with Growth Hormone Deficiency and Resistance." Case Reports in Pediatrics 2018 (2018): 1–4. http://dx.doi.org/10.1155/2018/5902835.
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By definition, about 2.5% of children show a short stature due to several causes. Two clinical conditions are characterized by serum IGF-I low levels, idiopathic GH deficiency (IGHD), and GH insensitivity (GHI), and the phenotypic appearance of these patients may be very similar. We studied two children with short stature and similar phenotypes. The first case showed frontal bossing, doll face, acromicria, and truncal obesity, with a GH peak <0.05 ng/ml after stimuli and undetectable serum IGF-I levels. After PCR amplification of the whole GH1 gene, type IA idiopathic GHD was diagnosed. The second case had cranium hypoplasia, a large head, protruding forehead, saddle nose, underdeveloped mandible, and a micropenis. Basal GH levels were high (28.4 ng/ml) while serum IGF-I levels were low and unchangeable during the IGF-I generation test. Laron syndrome was confirmed after the molecular analysis of the GH receptor (GHR) gene. IGHD type IA and Laron syndrome is characterized by opposite circulating levels of GH, while both have reduced levels of IGF-I, with an overlapping clinical phenotype, lacking the effects of IGF-I on cartilage. These classical cases show the importance of differential diagnosis in children with severe short stature.
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Arsov, Alexander, Kaloyan Petrov, and Penka Petrova. "Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis." Molecules 26, no. 18 (September 16, 2021): 5625. http://dx.doi.org/10.3390/molecules26185625.
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To adapt to various ecological niches, the members of genus Bacillus display a wide spectrum of glycoside hydrolases (GH) responsible for the hydrolysis of cellulose and lignocellulose. Being abundant and renewable, cellulose-containing plant biomass may be applied as a substrate in second-generation biotechnologies for the production of platform chemicals. The present study aims to enhance the natural cellulase activity of two promising 2,3-butanediol (2,3-BD) producers, Bacillus licheniformis 24 and B. velezensis 5RB, by cloning and heterologous expression of cel8A and cel48S genes of Acetivibrio thermocellus. In B. licheniformis, the endocellulase Cel8A (GH8) was cloned to supplement the action of CelA (GH9), while in B. velezensis, the cellobiohydrolase Cel48S (GH48) successfully complemented the activity of endo-cellulase EglS (GH5). The expression of the natural and heterologous cellulase genes in both hosts was demonstrated by reverse-transcription PCR. The secretion of clostridial cellulases was additionally enhanced by enzyme fusion to the subtilisin-like signal peptide, reaching a significant increase in the cellulase activity of the cell-free supernatants. The results presented are the first to reveal the possibility of genetic complementation for enhancement of cellulase activity in bacilli, thus opening the prospect for genetic improvement of strains with an important biotechnological application.
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Gerzson, Laís Rodrigues, Bruna Maciel Catarino, Kelly Andara de Azevedo, Paula Ribeiro Demarco, Míriam Stock Palma, and Carla Skilhan de Almeida. "Frequência semanal de um programa de intervenção motora para bebês de berçário." Fisioterapia e Pesquisa 23, no. 2 (June 2016): 178–84. http://dx.doi.org/10.1590/1809-2950/14923223022016.
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RESUMO O objetivo do estudo foi comparar o efeito de um Programa de Intervenção Motora no desenvolvimento de bebês de escolas de educação infantil públicas de Porto Alegre. Participaram do estudo 59 bebês, estratificados aleatoriamente em três grupos: 18 bebês atendidos três vezes por semana (G3X); 23 bebês atendidos uma vez por semana (G1X) e 18 bebês do grupo controle (GC). Foram realizadas tarefas de perseguição visual (três minutos), manipulação de objetos (sete minutos) e força, mobilidade e estabilização (dez minutos). O instrumento utilizado foi a Alberta Infant Motor Scale (AIMS) para avaliar o desenvolvimento motor dos bebês. Os resultados do estudo mostraram que os bebês do G1X foram os que melhoraram na classificação (p=0,007); nas posturas, foram os bebês do G3X que obtiveram diferença significativa maior na postura prono, sentado e em pé, mesmo sendo mais novos. Em conclusão, os bebês que realizaram intervenção motora, uma ou três vezes por semana, obtiveram melhores resultados quando comparados ao grupo controle.
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Vidal, Katherine, Allison D. Britt, Anu George, Joseph Ray, and Phillip D. K. Lee. "GH1 C.291 + 34G>C in a Growth Hormone Deficient Pedigree." Journal of the Endocrine Society 5, Supplement_1 (May 1, 2021): A558. http://dx.doi.org/10.1210/jendso/bvab048.1138.
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Abstract Background: Heterozygous loss of function mutations in GH1 are a cause of autosomal dominant isolated growth hormone deficiency (GHD) or IGHD type II. However, this condition is rare and a genotype/phenotype association is often based on single case or pedigree reports. Clinical Case: The male proband was born at 38 weeks following an uncomplicated pregnancy, birthweight 2585 gm (˗1.3SD), length 43 cm (˗2.7SD). At 20 months old: length ˗3.2SD, weight/length 16%. Peak growth hormone (GH) levels 4.42 ng/mL and 3.2 ng/mL following clonidine and arginine stimulation, respectively; cortrosyn-stimulated cortisol was 41.4 mcg/dL. Prolactin was 61.2 ng/mL (reference range 2.6 to 13.1 ng/mL); T4 and TSH levels were normal. Magnetic resonance imaging showed a flattened anterior pituitary gland, consistent with empty sella, with normal bright spot. He has had an intermittent and variable response to GH treatment due to noncompliance; recent recorded height was ˗3.6SD at 13.9 and he was early pubertal. Father had a history of childhood growth hormone treatment and is 151 cm tall, 2 paternal aunts did not receive treatment and are 145 cm tall, 3 children of these aunts are diagnosed with GHD. There is no maternal family history of GHD; mother is 155 cm. Proband testing revealed heterozygous GH1 c.291 + 34G>C (EGL Genetics), classified as a variant of unknown significance; father was also found to have this variant, mother was negative. Genetic testing for of additional family members is under consideration. Conclusion: Nine of 96 heterozygous GH1 variants are located in intron 3. Two have been classified as pathogenic and are predicted to cause exon skipping. Our proband’s variant cannot be considered a canonical splice site or in a mutational hot spot but has extremely low frequency in population databases. Functional studies clan clarify if our proband’s variant results in a 17.5 kDa GH isoform and dominant negative effect on full length GH production with potential detrimental effects on other anterior pituitary hormones. We present the second GHD pedigree with GH1 c.291 + 34G>C mutation. As in the previous report (1), no other hormone deficiencies are identified, despite the appearance of an empty sella for our proband. Reference: (1) Cho SY, Ki, CS, Park, HD, et al. Genetic investigation of patients with undetectable peaks of growth hormone after two provocation tests. Clin Endocrinol 2013; 78: 317-20.
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Zhou, Cuiqi, Stephen Shen, Rosemary Moran, and Shlomo Melmed. "Pituitary Somatotroph Adenoma Cell-Derived Exosomes: Characterization of Novel Non-Hormonal Functions." Journal of the Endocrine Society 5, Supplement_1 (May 1, 2021): A652—A653. http://dx.doi.org/10.1210/jendso/bvab048.1331.
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Abstract Exosomes, small extracellular vesicles carrying lipids, proteins, DNA and RNA, enable intercellular communication. Pituitary-derived exosomes have not been well validated, and as no human pituitary cell lines are available, we characterized exosomes derived from rat somatotroph tumor cells (GH1 and GH3). Rat FR and H9C2 cells were used as non-pituitary controls. Exosomes were isolated from serum-free culture supernatants by combining ultrafiltration and ultracentrifugation to eliminate hormone contamination. Derived exosomes were analyzed by NanoSight to visualize, size, and count particles. Exosomal proteins were extracted and exosome markers including TSG101, ALIX, CD63, HSP70, HSP90 detected by Western Blot. The exosome inhibitor GW4869 (10 µM, 30 h) reduced exosome release (up to 81%), whereas treating cells with hydrocortisone (0.1 µM, 72 h) increased exosome production (up to 42%) in GH1 and GH3 cells. Exosomal shuttle RNA characterized by RNA-Seq showed distinct pituitary vs non-pituitary exosome RNA profiles. Selected miRNAs assessed in exosomes and corresponding cells by qRT-PCR validated exosomal RNA-seq and suggested that miRNA signatures in exosomes and in respective cells of origin were concordant. Next, we explored downstream signaling of GH1-derived exosomes (GH1-exo) in vitro and in vivo and studied biological actions in normal hepatocytes and in malignant cells. As evidenced by mRNA-seq, GH1-exo distinctly altered signaling pathways in rat primary hepatocytes, vs pathways elicited by GH or PRL (0.5 µg/mL, 24 h). GH1-exo, FR-exo or vehicle were intravenously injected to 4-week-old female Wistar rats twice weekly for 4 weeks (5*109 exo/200 g, n=3), and livers dissected for mRNA-seq. Among GH1-exo specifically regulated genes, EIF2AK/ATF4, involved in cAMP responses and amino acid biosynthesis, were attenuated. In hepatocytes, GH1-exo suppressed up to 65% of nascent protein synthesis and reduced forskolin (10 µM)-stimulated cAMP activity by 19%, while GH (0.01-1 µg/mL) did not affect this pathway. Notably, GH1-exo also attenuated malignant cell motility. Both GH1-exo incubation or GH1 cell co-culture (48 h) suppressed migration, invasion and wound healing of HCT116 cancer cells by up to 70%. In contrast, treatment with rGH (0.5 µg/mL) increased HCT116 motility. Intravenous administration of GH1-exo (1010 exo/mouse, twice a week for 5 weeks) decreased metastatic tumor volume by 40% in nude mice harboring splenic HCT116 implants (5*105 cells/mouse, n=10), and especially abrogated hepatic metastases. mRNA-seq of GH1-exo treated HCT116 cells vs controls indicated dysregulated p53 and MAPK pathways, which may partially explain mechanisms underlying motility attenuation. The results elucidate novel biological actions of somatotroph adenoma cell-derived exosomes and suggest exosomes as non-hormonal messengers produced by pituitary tumors.
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Cota, Junio, Thamy L. R. Corrêa, André R. L. Damásio, José A. Diogo, Zaira B. Hoffmam, Wanius Garcia, Leandro C. Oliveira, Rolf A. Prade, and Fabio M. Squina. "Comparative analysis of three hyperthermophilic GH1 and GH3 family members with industrial potential." New Biotechnology 32, no. 1 (January 2015): 13–20. http://dx.doi.org/10.1016/j.nbt.2014.07.009.
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Pickett, C. A., and A. Gutierrez-Hartmann. "Epidermal growth factor and Ras regulate gene expression in GH4 pituitary cells by separate, antagonistic signal transduction pathways." Molecular and Cellular Biology 15, no. 12 (December 1995): 6777–84. http://dx.doi.org/10.1128/mcb.15.12.6777.
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We have previously demonstrated that epidermal growth factor (EGF) produces activation of the rat prolactin (rPRL) promoter in GH4 neuroendocrine cells via a Ras-independent mechanism. This Ras independence of the EGF response appears to be cell rather than promoter specific. Oncogenic Ras also produces activation of the rPRL promoter when transfected into GH4 cells and requires the sequential activation of Raf kinase, mitogen-activated protein (MAP) kinase, and c-Ets-1/GHF-1 to mediate this response. In these studies, we have investigated the interaction between EGF and Ras in stimulating rPRL promoter activity and the role of Raf and MAP kinases in mediating the EGF response. We have also examined the role of several transcription factors and used various promoter mutants of the rPRL gene in order to better define the trans- and cis-acting components of the EGF response. EGF treatment of GH4 cells inhibits activation of the rPRL promoter produced by transfection of V12Ras from 24- to 4-fold in an EGF dose-dependent manner. This antagonistic effect of EGF and Ras is mutual in that transfection of V12Ras also blocks EGF-induced activation of the rPRL promoter in a Ras dose-dependent manner, from 5.5- to 1.6-fold. Transfection of a plasmid encoding the dominant-negative Raf C4 blocks Ras-induced activation by 66% but fails to inhibit EGF-mediated activation of the rPRL promoter. Similarly, transfection of a construct encoding an inhibitory form of MAP kinase decreases the Ras response by 50% but does not inhibit the EGF response. Previous studies have demonstrated that c-Ets-1 is necessary and that GHF-1 acts synergistically with c-Ets-1 in the Ras response of the rPRL promoter. In contrast, overexpression of neither c-Ets-1 nor GHF-1 enhanced EGF-mediated activation of the rPRL promoter, and dominant-negative forms of these transcription factors failed to inhibit the EGF response. Using 5' deletion and site-specific mutations, we have mapped the EGF response to two regions on the proximal rPRL promoter. One region maps between -255 and -212, near the Ras response element, and a second maps between -125 and -54. The latter region appears to involve footprint 2, a previously identified repressor site on the rPRL promoter. Neither footprint 1 nor 3, known GHF-1 binding sites, appears to be crucial to RGF-mediated rPRL promoter activation. The results of these studies indicate that in GH4 neuroendocrine cells, rPRL gene regulation by EGF is mediated by a signal transduction pathway that is separate and antagonistic to the Ras pathway. Hence, the functional role of the Ras/Raf/MAP kinase pathway in mediating transcriptional responses to EGF and other receptor tyrosine kinase may differ in highly specialized cell types.
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Marques, Maria do Rosário, Ingrid C. Santos, Nuno Carolino, Carlos C. Belo, Robert Renaville, and Alfredo Cravador. "Effects of genetic polymorphisms at the growth hormone gene on milk yield in Serra da Estrela sheep." Journal of Dairy Research 73, no. 4 (July 7, 2006): 394–405. http://dx.doi.org/10.1017/s0022029906001932.
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The five exons and the 5′ and 3′-untranslated regions (5′-UTR and 3′-UTR) of the oGH gene were screened for mutations using PCR-single strand conformation polymorphism (PCR-SSCP) procedures in 523 Serra da Estrela ewes and were found to be highly polymorphic. The region extending across and between the GH2-N and GH2-Z copies was sequenced allowing the design of primers for the specific PCR amplification of each copy. These were cloned and sequenced in 20 animals representative of all SSCP patterns. The corresponding genotypes were established for each copy following nucleotide sequencing of SSCP alleles. Twenty-four polymorphic sites were found at the GH2-N (or GH1) and fourteen at the GH2-Z copies. Eight amino acid substitutions were predicted at the GH2-N and six at the GH2-Z copies. Milk yield adjusted to 150 lactation days was analysed for the genotype of each oGH gene copy taken separately or together (associated genotypes) by restricted maximum likelihood (REML) through a univariate best linear unbiased prediction (BLUP) animal model with repeated measures. Significant associations between genotypes and milk yield were observed. Within GH2-N genotypes there was a milk yield differential of 21·4±0·2 l/150 d between the most (N7) and the least (N5) productive ones. Within GH2-Z genotypes there was a differential of 21·6±0·2 l/150 d between the most (Z8) and the least (Z1) productive ones. The effect of associated GH2-N and GH2-Z genotypes revealed a differential of 39·6±0·3 l/150 d between the most (N1+Z7) and the least (N3+Z2) productive associated genotypes. The results show that GH2-N and GH2-Z genotypes significantly affect milk yield in Serra da Estrela ewes. Moreover, the apparent joint effect of GH2-N and GH2-Z genotype could improve milk yield in 25% as compared with the mean milk production of the analysed population.
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Van Vu, Ba, Kenji Itoh, Quoc Bao Nguyen, Yukio Tosa, and Hitoshi Nakayashiki. "Cellulases Belonging to Glycoside Hydrolase Families 6 and 7 Contribute to the Virulence of Magnaporthe oryzae." Molecular Plant-Microbe Interactions® 25, no. 9 (September 2012): 1135–41. http://dx.doi.org/10.1094/mpmi-02-12-0043-r.
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Upon infection, phytopathogenic fungi secrete an array of hydrolytic enzymes that can degrade components of the host epidermis, including waxes, the cuticle, and cell walls. Cellulases, which can hydrolyze crystalline cellulose in the plant cell wall, are among these hydrolytic enzymes. Here, we provide RNAi-based evidence to show that cellulases belonging to glycosyl hydrolase (GH) families 6 and 7 contribute to the penetration of the host epidermis and further invasion by the phytopathogenic fungus Magnaporthe oryzae. The GH6 and GH7 cellulases likely include all members of the cellobiohydrolase family and some endoglucanases in M. oryzae. Quantitative reverse-transcriptase polymerase chain reaction analysis indicated that more than half of the cellulases were highly induced during infection. We constructed knock-down (KD) mutants of these cellulases using the building blocks method we reported previously. The transcript levels of the target genes and cellulase activity were considerably reduced in the KD mutants. The KD mutants resulted in fewer lesions, less penetration, and infection of fewer cells compared with the parent strain. Cytological analyses showed that a high rate of papilla formation blocked invasion of the KD mutants into host cells. These results suggest that the GH6 and GH7 cellulases play roles in the virulence of M. oryzae.
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Ohta, S., Y. Shimekake, and K. Nagata. "Cell-type-specific function of the C-type natriuretic peptide gene promoter in rat anterior pituitary-derived cultured cell lines." Molecular and Cellular Biology 13, no. 7 (July 1993): 4077–86. http://dx.doi.org/10.1128/mcb.13.7.4077.
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The promoter function of the human C-type natriuretic peptide (CNP) gene in various cultured cells was examined by transient transfection assays. The CNP promoter functioned very effectively in GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, but functioned much less effectively in GH1 cells, another type of rat pituitary-derived cell with a somatotroph phenotype, and rat primary cardiocytes. The CNP promoter did not function at all in other cells, including AtT20 cells of murine pituitary corticotroph origin. Functional analyses of the deleted promoters with various 5' deletion breakpoints revealed the existence of at least two negative and one positive regulatory regions. Within the positive regulatory region (positions -54 to -19), which conferred 90% of the promoter activity in GH3 cells, two equipotent GC-rich cis elements (positions -49 to -45 and -40 to -35) were identified. Both sites shared half of the promoter activity and binding properties to the nuclear protein in GH3 cells. Rat anterior pituitary tissue contained the binding protein of the identified cis element, which was identical or similar to that of GH3 cells. With Southwestern (DNA-protein) analysis, a 70-kDa specific binding protein distinct from known factors such as SP-1, AP-2, and Pit-1 was identified in the nuclear extract of GH3 cells.
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Ohta, S., Y. Shimekake, and K. Nagata. "Cell-type-specific function of the C-type natriuretic peptide gene promoter in rat anterior pituitary-derived cultured cell lines." Molecular and Cellular Biology 13, no. 7 (July 1993): 4077–86. http://dx.doi.org/10.1128/mcb.13.7.4077-4086.1993.
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The promoter function of the human C-type natriuretic peptide (CNP) gene in various cultured cells was examined by transient transfection assays. The CNP promoter functioned very effectively in GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, but functioned much less effectively in GH1 cells, another type of rat pituitary-derived cell with a somatotroph phenotype, and rat primary cardiocytes. The CNP promoter did not function at all in other cells, including AtT20 cells of murine pituitary corticotroph origin. Functional analyses of the deleted promoters with various 5' deletion breakpoints revealed the existence of at least two negative and one positive regulatory regions. Within the positive regulatory region (positions -54 to -19), which conferred 90% of the promoter activity in GH3 cells, two equipotent GC-rich cis elements (positions -49 to -45 and -40 to -35) were identified. Both sites shared half of the promoter activity and binding properties to the nuclear protein in GH3 cells. Rat anterior pituitary tissue contained the binding protein of the identified cis element, which was identical or similar to that of GH3 cells. With Southwestern (DNA-protein) analysis, a 70-kDa specific binding protein distinct from known factors such as SP-1, AP-2, and Pit-1 was identified in the nuclear extract of GH3 cells.