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1
Rodriguez, Francisco Jose. "Oral mucositis in children receiving bone marrow transplantation." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2008m/rodriguez.pdf.
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Wickström, Claes. "MUC5B from the oral cavity identification of 'insoluble' assemblies and putative regulatory proteolytic events /." [Malmö] : Faculty of Odontology, Malmö University, 2002. http://catalog.hathitrust.org/api/volumes/oclc/51310732.html.
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Hansson, Annette. "Modeling of multi-step oral carcinogenesis in vitro : assessment of growth, differentiation and apoptosis markers /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-445-3/.
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Vondracek, Martin. "Toxicity of smokeless tobacco in human oral epithelium with emphasis on carcinogen metabolism and regulation of programmed cell death /." Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-335-X/.
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Ng, William Man Fai. "The effect of volatile thiol compounds on permeability of oral mucosa." Thesis, University of British Columbia, 1986. http://hdl.handle.net/2429/26508.
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Cumulative clinical and experimental evidence indicates that volatile sulphur compounds (VSC) the principal components of oral malodour, may play an important role in the pathogenesis of periodontal disease. As their (H₂S and CH₃SH) concentrations in gingival sulci increase with the severity of periodontal involvement, the objective of this investigation is to ascertain if they exert an effect on the permeability of oral mucosa.
Permeability determinations were performed on excised porcine sublingual mucosal specimens which consisted of non-keratinized epithelium, basal membrane and connective tissue layers mounted in a two compartment perfusion apparatus. Using radioactive and fluorescent-labelled penetrants, it was found that exposure of the epithelial surface to an atmosphere containing physiological concentrations of both thiols (15 ng H₂S or CH₃SH / ml of 95% air - 5% C0₂) increased the permeability of the mucosa to (³⁵S)-S0₄⁻², (³H)-prostaglandin E₂ (PGE₂) and fluorescein isothiocyanate labelled E. coli lipopolysaccharide (F-LPS). A three hour exposure of the mucosa to H₂S and CH₃SH resulted in a 75% and 103% increase respectively in permeability to (³⁵S)-labelled sulphate ion. Similarly, the mercaptan induced up to a 70% increase in permeability of the mucosa to (³H)-prostaglandin E₂. The
magnitude of changes in the permeability were found to depend on duration of exposure to the thiols and to their concentration. Studies using (³⁵S)-H₂S suggest that the observed changes in the tissue permeability are related to the reaction of the thiols with tissue components. In addition, the (³⁵S)-H₂S is capable of perfusing through all three layers of the mucosa at 12.3 ng / cm². In contrast to H₂S , the CH₃SH effect was irreversible in
control air / C0₂ environment. This infers that CH₃SH is
potentially a more deleterious agent to the tissue barrier. However, its effect can also be reversed by treatment of tissues with 0.22% ZnCl₂ either prior to or after exposure to mercaptan.
This suggests that Zn⁺² ion may be useful in preventing the potentially harmful effects of VSC.
Fluorescent studies with F-LPS indicate that thiols can also potentiate the penetration of endotoxin. Whereas the fluorescence of the F-LPS in control systems was confined to the superficial
epithelial layer in contact with the endotoxin, the CH₃SH-
exposed mucosa exhibited fluorescence throughout the epithelial and connective tissue layers. Fluorescent staining of the mucosal specimens with fluorescein diacetate followed by counter staining with ethidium bromide provides evidence of membrane impairment to some cells by CH₃SH. Collectively these
observations provide strong experimental evidence that the VSC, products of putrefaction produced in the gingival sulcus by oral microflora, may adversely affect the integrity of the crevicular barrier to deleterious agents and thus contribute to the etiology of periodontal disease.Dentistry, Faculty of
Graduate
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Nilsson, Jan Anders. "Aldehyde toxicity in human oral epithelium /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-141-5/.
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Nicander, Ingrid. "Electrical impedance related to experimentally induced changes of human skin and oral mucosa /." Stockholm, 1998. http://diss.kib.ki.se/search/diss.se.cfm?19980907nica.
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Mörée, Agnes. "Gold and mercury in the oral mucosa in patch-tested patients with oral lichen lesions." Thesis, Malmö högskola, Odontologiska fakulteten (OD), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19701.
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Rantanen, Irma. "Betaine in oral hygiene with special attention to dry and sensitive mucosa." Turku : Turun Yliopisto, 2003. http://books.google.com/books?id=qcJpAAAAMAAJ.
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Matheus, Paula Daniele. "Cell proliferation rate in clinically healthy oral mucosa of crack cocaine users." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/143365.
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Objetivo: Avaliar a taxa proliferativa de células esfoliadas da mucosa bucal clinicamente saudável de usuários de crack. Material e Métodos: esfregaços orais foram coletados de língua e assoalho bucal de 87 indivíduos, divididos em três grupos: os usuários de crack (CRCO), n = 26; fumantes / etilistas (SA), n = 26 e controles (C ), n = 35. Lâminas histológicas foram submetidas à técnica de impregnação pela prata para quantificação do número de AgNORs/núcleo. As imagens foram obtidas por um sistema de captura de imagem adaptado a um microscópio de luz em x1000 ampliação. A média AgNOR por núcleo (mAgnor) e a percentagem de células com mais de 1,2,3 e 4 AgNORs por núcleo (pAgNOR> 1,> 2> 3 um> 4) foram calculados. Resultados: As células esfoliadas de mucosa da língua SA (3,34 ± 0,51 AgNOR / núcleo) exibiram maior taxa de proliferação celular (p <0,05) quando comparado com C (2,81 ± 0,773 AgNORs / núcleo) e CRCO (2,87 ± 0,51 AgNORs / núcleo) . Um aumento (p <0,05) da mAgnor também foi observada nas células do assoalho bucal (3,55 ± 0,57) em comparação com SA C (3,18 ± 0,53) e CRCO (3,28 ± 0,39). Dados semelhantes foram encontrados usando pAgNOR>1,>2,>3 e > 4. Conclusão: usuários de crack não apresentaram alterações na taxa proliferativa celular da mucosa bucal. Diante dos dados apresentados, o consumo de cigarro, em combinação com o consumo de álcool, continua sendo o maior fator prejudicial à mucosa bucal.Objective: The aim of this study was to evaluate cell proliferation rate of cells exfoliated from clinically healthy mucosa of crack cocaine users. Material and Methods: Oral smears were collected from tongue and floor of the mouth mucosa of 87 individuals divided into three groups: crack cocaine users (CrCo), n=26; smokers/alcohol drinkers (SA), n=26 and controls (C), n=35. Histological slides were silver-stained using AgNOR technique to evaluate cell proliferation rate. Images were obtained by an image capturing system adapted to a light microscope at x1000 magnification. Quantification considered 50 cells by smear in which the number of AgNOR dots was visually counted. Mean AgNOR numbers per nucleus (mAgNOR) and the percentage of cells with more than 1,2,3 and 4 AgNORs per nucleus (pAgNOR>1,>2>3 an>4) were calculated. Results: Cells exfoliated from tongue mucosa of SA (3.34±0.51 AgNOR/nucleus) exhibit higher cell proliferation rate (p<0.05) when compared to C (2.81±0.773 AgNORs/nucleus) and to CrCo (2.87±0.51 AgNORs/nucleus). An increase (p<0.05) in mAgNOR was also observed in floor of the mouth cells (3.55±0.57) in SA when compared to C (3.18±0.53) and CrCo (3.28±0.39). Similar findings were found using pAgNOR>1,>2,>3 e >4. Conclusion: Crack cocaine users did not present changes in cell proliferation rate of oral mucosa. Between the expositions studied here, cigarette smoking in combination with alcohol consumption remain as the most harmful factors to oral mucosa.
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Kautsky, Mikael B. "The role of retinoic acid receptors in oral epithelial differentiation /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/6395.
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Arêde, Lívia Trevelin. "Detecção do vírus Epstein-Barr em carcinoma espinocelular oral e mucosa oral na região de Araçatuba - SP, Brasil /." Araçatuba, 2010. http://hdl.handle.net/11449/91429.
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Orientador: Glauco Issamu MiyaharaCoorientador: José Fernando Garcia
Banca: Eder Ricardo Biasoli
Banca: Luiz Alberto Veronese
Resumo: O vírus Epstein-Barr (EBV) faz parte da família herpesvirus humano e está relacionado com doenças benignas e malignas de cabeça e pescoço e sua possível relação com o carcinoma espinocelular (CEC) oral tem sido estudada. O objetivo deste estudo foi detectar a presença do EBV em pacientes com CEC oral e mucosa normal, correlacionando os achados com as variáveis clínico-patológicas, fatores de risco e sobrevida. Foi aplicada a nested-PCR em peças parafinizadas de CEC oral, sendo 20 amostras de assoalho bucal, 25 de língua e 12 de orofaringe. Também foi utilizado grupo controle, sem carcinoma espinocelular, com 19 amostras de mucosa oral normal. O vírus foi encontrado em 10% das amostras de assoalho bucal e 12% de língua, não sendo detectado na orofaringe. Para as amostras de mucosa normal a prevalência foi de 15,79%. Não houve diferença estatisticamente significante entre a presença do vírus e as variáveis: localização anatômica, sexo, idade, tabagismo, etilismo, estadiamento clínico, gradação histológica, esvaziamento cervical e sobrevida. Os resultados sugerem que o EBV não participa isoladamente da carcinogênese oral
Abstract: The Epstein-Barr virus (EBV) is part of human herpesvirus family being associated with benign and malignant diseases head and neck. Its possible relation with oral squamous cell carcinoma (OSCC) has been studied. The aim of this study was to detect the presence of EBV in patients with OSCC and normal mucosa, correlating the findings with clinicopathologic variables, risk factors and patient's survival. Nested PCR was applies in paraffin embedded samples of OSCC being 20 samples of mouth floor, 25 tongue and 12 oropharyngeal. A control group composed of 19 normal oral mucosa samples was used. The virus was found in 10% of mouth floor samples, 12% of tongue not being detected in oropharynx. The prevalence in oral mucosa samples was 15.79%. There was no statistically significant differences between the virus presence and the variables: anatomical localization, gender, age, smoking, alcoholism, clinical stage, histological grade, neck dissection and patient's survival. The results suggest that EBV alone did not have participation in carcinogenesis or oral SCC
Mestre
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Souza, Ana Clara de. "Caracterização e relação de leveduras do gênero Candida isoladas das mucosas oral e vaginal de mulheres com lesões causadas por HPV de alto risco para câncer do colo do útero." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/23/23139/tde-03072017-155030/.
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Este estudo caracterizou e relacionou as leveduras do gênero Candida isoladas das mucosas oral e vaginal de mulheres com lesões causadas por HPV de alto risco para câncer do colo do útero. Foram examinadas 42 mulheres tratadas no ambulatório de Patologia do Trato Genital Inferior do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo sendo, 30 com lesões uterinas de alto grau (G1) com média de idade de 36,5 anos ± 11,1 e 12 com lesões uterinas de baixo grau (G2) com média de idade de 34,75 anos ± 15,5. Condições clínicas e dados laboratoriais sobre HPV foram coletados do prontuário médico das pacientes; os dados sócio-demográficos obtidos a partir de um questionário apropriado. Para o estudo de associação entre as variáveis foi utilizada a análise de razão de chance (Odds Ratio) a partir do programa STATA 13.1. Foram identificadas associação entre lesões uterinas de baixo grau com cultura positiva em mucosa oral (OR= 0,215) e com presença de doenças crônicas (OR = 0,167), sendo que pacientes com lesões uterinas de alto grau possuem maior prevalência para diabetes e os resultados indicaram 23% de prevalência de Candida spp. em mucosa oral e 27% em mucosa vaginal, em pacientes do G1,no G2 foi de 42% em mucosa oral e de 33% em mucosa vaginal. Entre as espécies encontradas em mucosa oral e vaginal das pacientes, Candida albicans foi a mais isolada com 88%, seguida de C. tropicalis (8%)e C. glabrata (4%). As cepas de C. albicans isoladas de ambas as mucosas apresentaram sensibilidade a todos os antifúngicos testados, ao contrário da cepa de C. tropicalis isolada no Grupo 2, em mucosa vaginal, que apresentou um perfil de resistência ao fluconazol. Assim, torna-se importante o acompanhamento e supervisão por meio de exames clínicos e laboratoriais das pacientes com HPV, reforçando a necessidade sobre cuidados, tratamento e prevenção de infecções relacionadas ao HPV e a Candida spp.This study characterized and related yeasts of the genus Candida isolated from the oral and vaginal mucous membranes of women with lesions caused by high-risk HPV for cervical cancer. Forty-two women treated at the Lower Genital Tract Pathology Clinic of the University of São Paulo Medical School\'s Hospital of Clinics were examined, with 30 high-grade (G1) uterine lesions with a mean age of 36.5 years ± 11, 1 and 12 with low grade (G2) uterine lesions with a mean age of 34.75 years ± 15.5. Clinical conditions and laboratory data on HPV were collected from patients\' medical records; the socio-demographic data obtained from an appropriate questionnaire. For the study of association between the variables, Odds Ratio analysis was used from the STATA 13.1 program. An association between low grade uterine lesions with positive culture in oral mucosa (OR = 0.215) and presence of chronic diseases (OR = 0.167) was identified. Patients with high grade uterine lesions had a higher prevalence for diabetes and the results indicated 23% prevalence of Candida spp. In oral mucosa and 27% in vaginal mucosa, in G1 patients, in G2 it was 42% in oral mucosa and 33% in vaginal mucosa. Among the species found in oral and vaginal mucosa of patients, Candida albicans was the most isolated with 88%, followed by C. tropicalis (8%) and C. glabrata (4%). The strains of C. albicans isolated from both mucosa presented sensitivity to all tested antifungal agents, unlike the C. tropicalis strain isolated in Group 2, in vaginal mucosa, which presented a resistance profile to fluconazole. Thus, monitoring and supervision through clinical and laboratory testing of HPV patients is important, reinforcing the need for care, treatment and prevention of HPV-related infections and Candida spp.
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Destro, Maria Fernanda de Sousa Setubal. "Analise dos niveis de expressão dos membros da familia HOX de genes homeobox localizados nos loci B e C em mucosa oral normal e carcinoma espinocelular oral." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288726.
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Orientador: Ricardo Della ColettaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Genes homeobox transcrevem fatores de transcrição com participação importante na organogênese através do controle da proliferação e diferenciação celular. Dentre estes genes destacam-se os membros da família HOX de genes homeobox, os quais estão envolvidos em processos celulares cruciais como controle do ciclo celular, diferenciação e apoptose. Os genes HOX estão relacionados com o surgimento de diferentes tipos de neoplasias, incluindo cânceres de mama, ovário, próstata, rins, pulmão, pele e leucemias. Na cavidade oral a participação destes genes é desconhecida. O objetivo deste estudo foi comparar os níveis de expressão dos membros da família HOX de genes homeobox dos loci B e C entre amostras orais de mucosa normal e carcinoma espinocelular (CEC). Para a realização deste trabalho, amostras de mucosa oral normal de pacientes não expostos aos principais fatores de risco para o câncer oral (hábito de fumar e consumir bebidas alcoólicas) e amostras orais de mucosa normal e CEC provenientes do mesmo paciente foram submetidos a ensaios semi-quantitativos de transcriptase reversa-reação em cadeia da polimerase (RT-PCR) ¿duplex¿, utilizando-se primers para o gene controle GAPDH e primers específicos para cada um dos membros dos loci B e C. Em adição, os níveis de expressão destes genes foram analisados em uma linhagem de queratinócito normal (HaCAT) e em 4 linhagens celulares derivadas de CEC oral. As linhagens celulares de CEC oral expressaram todos os membros do loci C, sendo que os genes HOXC4, HOXC5 e HOXC6 apresentaram maiores níveis de expressão quando comparado com a linhagem HaCAT. HOXB13 foi expresso por todas as linhagens celulares de CEC oral. Os genes HOXB1, HOXB3, HOXB5, HOXB8 e HOXC12 não foram expressos por nenhuma das amostras de mucosa oral normal, independente da origem, e de CEC oral. O padrão de expressão dos genes HOX foi muito similar entre os dois grupos de mucosa oral normal. As expressões dos membros HOXB7, HOXC4, HOXC5, HOXC6, HOXC8, HOXC9, HOXC10 e HOXC11 foram significantemente maiores nas amostras de CEC oral comparado com amostras de mucosa oral normal. Os níveis de expressão dos membros HOXB2 e HOXC13 foram significantemente maiores nas amostras de CEC oral quando comparado com os níveis de expressão encontrados nas amostras de mucosa normal de pacientes livres de fatores de risco. Estes resultados sugerem que a expressão alterada de alguns membros da família HOX de genes homeobox pode estar associada com o desenvolvimento e/ou progressão do CEC oral
Abstract: Homeobox genes encode transcription factors with an important role during normal development by controlling cellular proliferation and differentiation. Among those genes, the HOX family is involved in crucial biological processes such as the control of the cell cycle, differentiation and apoptosis. HOX genes are related to many cancers, including those of the breast, ovary, prostate, kidney, lung, skin and leukemias. In the oral cavity, the role of those genes is unclear. The aim of this study was to compare the expression levels of HOX genes from loci B and C between normal oral mucosa and oral squamous cell carcinoma (SCC). Samples of normal oral mucosa and oral SCC obtained from the same patient, and samples of normal oral mucosa from patients without history of exposition to risk factors related to oral SCC (smoking habit and alcohol consumption) were analyzed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) duplex method with specific primers for the control gene GAPDH and for each of the HOXB and HOXC members. Additionally, we analyzed the expression profile of those genes in a normal keratinocyte cell line (HaCAT) and 4 oral SCC cell lines. Oral SCC cell lines expressed all members of the locus C, and the expression of HOXC4, HOXC5 and HOXC6 was higher in those cell lines compared with HaCAT. Only HOXB13 was expressed for all oral SCC cell lines. None of the normal oral mucosa and oral SCC samples expressed HOXB1, HOXB3, HOXB5, HOXB8 and HOXC12. The HOX expression profile of the 2 groups of normal oral mucosa was quite similar. Regardless of the oral normal mucosa source, the expression of HOXB7, HOXC4, HOXC5, HOXC6, HOXC8, HOXC9, HOXC10 and HOXC11 was statistically higher in oral SCC samples. HOXB2 and HOXC13 were significantly overexpressed in oral SCC when compared with normal oral mucosa from patients without risk factors related to oral SCC. These results suggest that a dysregulated expression of HOX genes from clusters B and C may be related to the tumorigenesis and/or tumor progression of oral SCCs
Mestrado
Patologia
Mestre em Estomatopatologia
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Thurfjell, Niklas. "p63 - from expression to function : studies of normal oral mucosa and squamous cell carcinoma of the head and neck /." Doctoral thesis, Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-998.
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Silva, Wellington Luiz Ferreira da. "Interação do Paracoccidioides brasiliensis com células dendríticas e queratinócitos em biopsias de lesões de pele e mucosa oral." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5133/tde-09082016-151216/.
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A paracoccidioidomicose (PCM) é uma doença sistêmica causada pelos fungos Paracoccidioides brasiliensis e Paracoccidioideslutzii. O comprometimento da pele e mucosa oral são frequentes na PCM. As células dendríticas e queratinócitos do tegumento, devido à sua função como células apresentadoras de antígenos, atuam na resposta imune inata e adaptativa contra agentes patogênicos. Com o objetivo de verificar a interação do P. brasiliensis com essas células, estudamos 47 biopsias de mucosa oral e 52 de pele de lesões de doentes com diagnóstico comprovado de PCM. As biopsias foram submetidas à técnica de imuno-histoquímica de dupla-marcação com os anticorpos anti-fator XIIIa (marcador de dendrócitos dérmicos), anti-CD207 (marcador de células de Langerhans maduras), anti-pancitoqueratinas (AE1-AE3) e anti-P. brasiliensis. Fez-se também a reação de dupla marcação por técnica de imunofluorescência, com análise por microscopia confocal a laser, para a melhor visualização da interação entre queratinócitos e os fungos. Quarenta e dois por cento das amostras de mucosa oral exibiram formas fúngicas no citoplasma de dendrócitos dérmicos. As células de Langerhans, tanto nas biopsias de mucosa oral como de pele, não mostraram leveduras ou antígenos do fungo no seu citoplasma. Cinquenta e quatro por cento das biopsias de pele e sessenta por cento das amostras de mucosa exibiram leveduras no citoplasma de queratinócitos. Os resultados obtidos permitem concluir que o parasitismo de queratinócitos pode representar possível mecanismo de evasão do fungo aos mecanismos imunes locais. Os dendrócitos dérmicos fator XIIIa positivos e queratinócitos podem estar a atuar como células apresentadoras de antígenos para suprir a função, provavelmente prejudicada, das células de Langerhans nas lesões de pele e mucosa oral da PCM humanaParacoccidioidomycosis (PCM) is a systemic disease caused by the fungus Paracoccidioides brasiliensis, which compromises various organs, mainly the lungs. The skin and oral mucosa are often affected. Dendritic cells and keratinocytes of the integument play a role in innate and adaptive immune response against pathogens, due to their function as antigen presenting cells. Aiming to verify the interaction of P. brasiliensis with these cell populations, we studied 52 biopsies of skin and 47 oral mucosa samples taken from patients with proven diagnosis of PCM. The biopsies were subjected to double immune staining technique with anti-factor XIIIa (marker of dermal dendrocytes), anti- CD207 (marker of mature Langerhans cells), anti-pan cytokeratins (AE1-AE3) and anti P. brasiliensis antibodies. Analyses with confocal laser microscopy were also performed to better visualization of the interaction between keratinocytes and the fungi. Factor XIIIa + dermal dendrocytes of 42% samples of oral mucosa displayed yeast forms in their cytoplasm. We did not observe yeast cells in the cytoplasm of Langerhans cells in both skin and oral mucosa samples. Fifty -four percent of skin and 60% of mucosal samples displayed yeast cells in the cytoplasm of keratinocytes. The parasitism of keratinocytes may represent a possible mechanism of evasion of the fungus to local immune mechanisms, or even as a result of keratinocytes ability to antigen presentation in PCM. Factor XIIIa dendrocytes and keratinocytes may be acting as antigenpresenting cells to fulfill the function of Langerhans cells, probably impaired, in skin and mucosa of human PCM
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Cury, Sérgio Elias Vieira. "Expressão imunoistoquímica das citoqueratinas 7 e 8 em carcinomas epidermóides de assoalho bucal." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-09042009-122159/.
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Objetivo: Verificar a presença da expressão imunoistoquímica das Citoqueratinas (CKs) 7 e 8 nas células do Carcinoma epidermóide localizado em assoalho bucal (CEAB), estabelecendo a especulação de que essa neoplasia tem origem no epitélio de transição entre o ducto excretor da glândula salivar e a mucosa oral de superfície. Métodos: Noventa e dois casos de CEAB foram avaliados através da técnica imunoistoquímica da streptavidina/biotina, utilizando-se anticorpos monoclonais anti-CKs 7 e 8. Resultados: Setenta e oito casos (84,78%) mostraram positividade para as CKs estudadas. Quarenta e cinco casos (48,9%) mostraram positividade para CK7 e Sessenta seis casos (71,7%) para CK8. Conclusão: Houve uma intensa marcação celular das CKs estudadas. Os resultados encontrados indicam a participação do epitélio de transição entre os ductos excretores das glândulas salivares e a mucosa bucal de revestimento, na gênese dos carcinomas epidermóides nessa localização. Objetivo: Verificar a presença da expressão imunoistoquímica das Citoqueratinas (CKs) 7 e 8 nas células do Carcinoma epidermóide localizado em assoalho bucal (CEAB), estabelecendo a especulação de que essa neoplasia tem origem no epitélio de transição entre o ducto excretor da glândula salivar e a mucosa oral de superfície. Métodos: Noventa e dois casos de CEAB foram avaliados através da técnica imunoistoquímica da streptavidina/biotina, utilizando-se anticorpos monoclonais anti-CKs 7 e 8. Resultados: Setenta e oito casos (84,78%) mostraram positividade para as CKs estudadas. Quarenta e cinco casos (48,9%) mostraram positividade para CK7 e Sessenta seis casos (71,7%) para CK8. Conclusão: Houve uma intensa marcação celular das CKs estudadas. Os resultados encontrados indicam a participação do epitélio de transição entre os ductos excretores das glândulas salivares e a mucosa bucal de revestimento, na gênese dos carcinomas epidermóides nessa localização.Objetive: The aim of this study is to verify the presence of Citokeratin7 (CK7) and Citokeratin 8 (CK8) positive cells of OSCC of the floor of the mouth establishing a speculation if this neoplasia has origin in excretory salivary duct epithelia transition to floor of the mouth mucosa. Methods: Ninety two cases of OSCC of the floor of the mouth were evaluated by straptavidin/biotin complex immunohistochemical technique and anti-CK7 and anti-CK8 monoclonal antibodies were used. Results: Seventy eight cases (84,78%) showed positive for one of the CKs; Forty five cases (48,9%) showed positive for CK7 and Sixty six cases (71,7%) showed positive for CK8. Conclusions: There is a high and significantly incidence of the immunoexpression of this citokeratins. These results suggest that the excretory salivary duct epithelia transition to floor of the mouth mucosa participates on the genesis of the OSCC in this location.
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Oliveira, Luisa Jardim Corrêa de. "Prevalência das lesões de mucosa bucal e seu impacto na qualidade de vida relacionada à saúde bucal em crianças de cinco anos de idade." Universidade Federal de Pelotas, 2014. http://repositorio.ufpel.edu.br/handle/ri/2304.
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Previous issue date: 2014-03-29Oral mucosal lesions are conditions that occur in soft tissues of the mouth, and are expressed by several clinical aspects. The literature has reported the need of considering the functional and psychosocial dimensions in regard to the oral health for the evaluation of dental interventions, such as the oral health related quality of life impact. In this context, the oral mucosal lesions can influence people's daily life due to pain and discomfort that they can cause. The aim of this study was to access the prevalence of oral mucosal lesions and their impact on Oral Health Related Quality of Life (OHRQoL) in children aged 5, from a birth cohort of Pelotas, Brazil. In 2009, a sample of 1,303 children born in Pelotas (2004 Cohort) was selected to participate in the study. Data were collected using a questionnaire applied to mothers and with clinical examinations of the children in their household. Oral mucosal lesions (OML) were identified by type, site and size. Early Childhood Oral Health Impact Scale (ECOHIS), consists of 13 questions, was used to assess caregivers perception on children OHRQoL. Descriptive analysis was performed in order to get the absolute and relative frequencies of the variables related to oral mucosal lesions. Bivariate analysis was performed to assess the association between the presence of OML and prevalence, extent and intensity of the ECOHIS items. Poisson regression models were used to investigate the association between lesions of the oral mucosa and ECOHIS score adjusting for confounders. The prevalence of the oral mucosal lesions was 30.1% (95% CI 27.5-32.9). The site more affected was the gum (31.0%) followed by the tongue (23.9%). Ulcers (29.4%) and papule/nodule (21.9%) were the more prevalent types of lesion. The majority of OML (76.8%) had size up to 5mm. A positive association was found between the presence of OML and impact on oral heath related quality of life measured in overall ECOHIS score (p <0.001), extent (p <0.001), prevalence (p = 0.002) and intensity (p = 0.010). Through the Poisson regression was observed that, even after adjustments, children with OML showed greater oral heath related quality of life (RR 95% CI 1:38 1:11; 1.72) than children without OML. Therefore, the main conclusions of this study are: there is a high prevalence of oral mucosal lesions in children 5 years old and these lesions impaired children oral health related quality of life
Lesões de mucosa bucal são condições que ocorrem nos tecidos moles da boca, e que se expressam por aspectos clínicos diversos. Tem sido apontada na literatura a necessidade de considerar as dimensões funcionais e psicossociais da saúde bucal para a avaliação de intervenções odontológicas, como o impacto na qualidade de vida relacionado à saúde bucal. Neste contexto, as lesões de mucosa bucal podem influenciar a vida diária das pessoas devido à dor e ao desconforto que podem causar. Assim, o objetivo deste estudo foi avaliar a prevalência das lesões de mucosa bucal e seu impacto na qualidade de vida relacionada à saúde bucal em crianças de 5 anos de idade pertencentes a uma coorte de nascimentos. Em 2009, uma amostra de 1.303 crianças nascidas em Pelotas e pertencentes à Coorte de 2004 foi selecionada para participar do estudo. Foi realizada a aplicação de um questionário e exames odontológicos no domicílio das crianças. As lesões foram identificadas no exame bucal segundo o tipo de lesão fundamental, localização e tamanho. O impacto na qualidade de vida relacionada à saúde bucal foi investigado através do instrumento Early Childhood Oral Health Impact Scale ECOHIS, respondido pelos responsáveis pela criança, e composto por 13 itens. Os dados obtidos foram analisados utilizando o software STATA 11.0. Análise descritiva foi realizada a fim de obterem-se as frequências relativas e absolutas das variáveis relativas às lesões de mucosa bucal. Análise bivariada foi realizada para verificar associação entre presença de lesões de mucosa bucal e prevalência, extensão e intensidade de respostas do ECOHIS. Modelos de regressão de Poisson foram utilizados pra verificar a associação entre lesões de mucosa bucal e o escore do ECOHIS ajustando por fatores de confusão. A prevalência das lesões de mucosa bucal foi de 30.1% (IC 95% 27.5-32.9). O sítio mais acometido foi a gengiva (31.0%), seguido da língua (23.9%). Os tipos de lesões fundamentais mais prevalentes foram as úlceras (29,4%) e as pápulas/nódulos (21,9%). A maioria das lesões (76.8%) tinha tamanho de até 5mm. Foi encontrada associação entre a presença de lesões de mucosa bucal e impacto na qualidade de vida relacionada à saúde bucal, medido em escore total médio do ECOHIS (p<0,001); extensão (p<0,001); prevalência (p=0,002) e intensidade (p=0,010). Através da regressão de Poisson foi observado que, mesmo após ajustes, crianças com lesão de mucosa bucal apresentaram maior impacto na qualidade de vida relacionada à saúde bucal (RR 1.38 95% CI 1.11; 1.72) do que crianças sem lesão de mucosa bucal. Assim, as principais conclusões dessa dissertação são: a prevalência de lesões de mucosa bucal em crianças de 5 anos de idade é alta e estas lesões causam um impacto negativo na qualidade de vida relacionada à saúde bucal.
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Sukotjo, Cortino. "Wit 3.0, a novel gene derived from edentulous oral mucosa, encodes cytoplasmic molecules facilitating oral mucosa wound contraction." Restricted to subscribing institutions, 2002. http://proquest.umi.com/pqdweb?did=1568361991&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.
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Luomanen, Marita. "Effect of CO2 laser surgery of rat mouth mucosa." Helsinki : Painovalssi Oy : [Distributed by] Akateeminen Kirjakauppa, 1987. http://books.google.com/books?id=4OZpAAAAMAAJ.
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Thesis--University of Finland, 1987.At head of title: Department of Cariology and Department of Pathology, University of Helsinki, Finland. Abstract sheet inserted. "Also published in Proceedings of the Finnish Dental Society, 1987, vol. 83, Suppl. XII"--T.p. verso. Includes bibliographical references (p. 60-70).
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Kinikoglu, Beste F. "Tissue Engineering Of Full-thickness Human Oral Mucosa." Phd thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/12612770/index.pdf.
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Tissue engineered human oral mucosa has the potential to fill tissue deficits caused by facial trauma or malignant lesion surgery. It can also help elucidate the biology of oral mucosa and serve as an alternative to in vivo testing of oral care products. The aim of this thesis was to construct a tissue engineered full-thickness human oral mucosa closely mimicking the native tissue. To this end, the feasibility of the concept was tested by co-culturing fibroblasts and epithelial cells isolated from normal human oral mucosa biopsies in a collagen-glycosaminoglycan-chitosan scaffold, developed in our laboratory to construct a skin equivalent. An oral mucosal
equivalent closely mimicking the native one was obtained and characterized by histology, immunohistochemistry and transmission electron microscopy. Using the same model, the influence of mesenchymal cells on oral epithelial development was investigated by culturing epithelial cells on lamina propria, corneal stroma and dermal equivalents. They were found to significantly influence the thickness and the ultrastructure of the epithelium. Finally, in order to improve the adhesiveness of conventional scaffolds, an elastin-like recombinamer (ELR) containing the cell adhesion tripeptide, RGD, was used in the production of novel bilayer scaffolds employing lyophilization and electrospinning. These scaffolds were characterized by
mercury porosimetry, scanning electron microscopy and mechanical testing. In vitro tests revealed positive contribution of ELR on the proliferation of both fibroblasts and epithelial cells. It was thus possible to construct a viable oral mucosa equivalent using the principles of tissue engineering.
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Kinikoglu, Fatma Beste. "Tissue engineering of full-thickness human oral mucosa." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10310.
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L’ingénierie de la muqueuse orale humaine (MOH) a pour but le comblement des pertes de substances suite à un traumatisme facial ou à la chirurgie des lésions malignes. Elle a aussi des applications en recherche pour élucider les mécanismes biologiques de la MO et en pharmacotoxicologie comme alternative à l’expérimentation animale. L'objectif de cette thèse était de reconstruire une MOH proche du tissu normal. À cette fin, la faisabilité du concept a d'abord été testée par co-culture de fibroblastes de la lamina propria et de cellules épithéliales de MOH dans le substrat de collagène-chitosan glycosaminoglycane, développé pour la production de peaux reconstruites. La caractérisation de la MOH reconstruite par histologie, immunohistochimie et microscopie électronique à transmission a montré la présence d’une LP équivalente avec un épithélium pluristratifié et non kératinisé très proche du tissu d’origine. Grâce à ce modèle, nous avons ensuite démontré que l’origine des fibroblastes (MO, cornée, peau) influence significativement l’épaisseur et l’ultrastructure de l'épithélium obtenu par culture de cellules épithéliales orales. Enfin, afin d'améliorer les propriétés adhésives du substrat à base collagène, nous avons ajouté au collagène, une élastine-like recombinante (ELR) contenant le tri-peptide d’adhésion cellulaire, RGD, et produit un nouveau substrat bicouche, poreux par lyophilisation et recouvert d’une couche fibreuse par électrofilage. Ces substrats ont été caractérisés par porosimétrie au mercure, microscopie électronique à balayage et essais mécaniques. Nous avons démontré l’effet stimulant de ELR sur la prolifération des fibroblastes et des cellules épithélialesTissue engineered human oral mucosa has the potential to fill tissue deficits caused by facial trauma or malignant lesion surgery. It can also help elucidate the biology of oral mucosa and serve as an alternative to in vivo testing of oral care products. The aim of this thesis was to construct a tissue engineered full-thickness human oral mucosa closely mimicking the native tissue. To this end, the feasibility of the concept was tested by co-culturing fibroblasts and epithelial cells isolated from normal human oral mucosa biopsies in a collagen-glycosaminoglycan-chitosan scaffold, developed in our laboratory to construct a skin equivalent. An oral mucosal equivalent closely mimicking the native one was obtained and characterized by histology, immunohistochemistry and transmission electron microscopy. Using the same model, the influence of mesenchymal cells on oral epithelial development was investigated by culturing epithelial cells on lamina propria, corneal stroma and dermal equivalents. They were found to significantly influence the thickness and the ultrastructure of the epithelium. Finally, in order to improve the adhesiveness of conventional scaffolds, an elastin-like recombinamer (ELR) containing the cell adhesion tripeptide, RGD, was used in the production of novel bilayer scaffolds employing lyophilization and electrospinning. These scaffolds were characterized by mercury porosimetry, scanning electron microscopy and mechanical testing. In vitro tests revealed positive contribution of ELR on the proliferation of both fibroblasts and epithelial cells. It was thus possible to construct a viable oral mucosa equivalent using the principles of tissue engineering
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Gibson, Rachel J. "Chemotherapy-induced mucositis : mechanisms of damage, time course of events and possible preventative strategies /." Title page, table of contents and abstract only, 2004. http://web4.library.adelaide.edu.au/theses/09PH/09phg4481.pdf.
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Berglundh, Tord. "Studies on gingiva and periimplant mucosa in the dog." Göteborg : Faculty of Odontology, University of Göteborg, 1993. http://catalog.hathitrust.org/api/volumes/oclc/29343307.html.
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Öhman, Sven-Christer. "Angular cheilitis a clinical microbiological and immunohistochemical study /." Gẗeborg : University of Gothenburg, Fauclty of Odontology, 1988. http://catalog.hathitrust.org/api/volumes/oclc/18422502.html.
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Hilliges, Marita. "Studies on nerve terminations in human mucosa and skin /." Stockholm, 1997. http://diss.kib.ki.se/1997/91-628-2649-2.
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Roosaar, Ann. "Oral mucosal lesions, tobacco use and the long-term outcome in a Swedish population /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-973-4/.
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ALBUQUERQUE, Monica Regina Barros de Moura. "Melanoma Maligno de Mucosa da Região de Cabeça e Pescoço e de Mucosa Oral: Frequência Relativa e Estudo Clínicopatológico." Universidade Federal de Pernambuco, 2012. https://repositorio.ufpe.br/handle/123456789/12650.
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Introdução: O melanoma maligno mucoso de cabeça e pescoço é uma neoplasia rara, consistindo em cerca de 0,2% a 8% dos melanomas que acometem outras localizações do corpo. A maior ocorrência do melanoma mucoso oral é, em média, de 41 a 60 anos de idade. A patogênese da doença é desconhecida e os seus principais fatores prognósticos são: estágio da doença; profundidade de invasão; invasão vascular; necrose; população de células tumorais polimórficas; aumento da idade; desenvolvimento de metástases linfonodais distantes; ulceração; locais anatômicos; pigmentação melânica. Os locais mais acometidos da cabeça e pescoço são as cavidades oral e nasal, as regiões do palato duro, gengiva e rebordo alveolar. Objetivo: Verificar a frequência relativa do melanoma maligno de mucosa de cabeça e pescoço (cavidade oral, rinofaringe, orofaringe e seio maxilar) e suas características clínico-patológicas em um período de 20 anos (1991 a 2010) em hospital de referência para tratamento de câncer no Estado de Pernambuco. Métodos: A coleta de dados ocorreu no Departamento de Patologia do Hospital de Câncer de Pernambuco (HCP) no período compreendido entre outubro de 2010 a dezembro de 2011. Foram obtidos 889 casos de melanoma cutâneo e mucoso em diversas regiões anatômicas. Através de consulta aos laudos e prontuários dos pacientes e, posteriormente, da obtenção de blocos de parafina e lâminas, foram excluídos os casos de melanomas cutâneos, os de melanomas mucosos em regiões que não foram em cabeça e pescoço e os casos que representaram lesões secundárias a partir de primários cutâneos. Foram selecionados para o presente estudo 08 casos de melanoma mucoso em cabeça e pescoço. As lâminas histológicas foram analisadas quanto aos aspectos dos elementos histopatológicos que interferem no prognóstico. Todos os dados clínicos e anatomopatológicos aferidos foram registrados em uma ficha padrão, tendo por base o protocolo padrão da Sociedade Brasileira de Patologia (2005). Resultados: Dos 08 casos selecionados (0,90%), observou-se a predominância da lesão na faixa etária entre 51 e 60 anos e entre 71 e 80 anos, com a proporção de 1:1 entre os sexos. A maior ocorrência da lesão foi na região do lábio: lábio superior (37,5%) e lábio inferior (25%), mucosa jugal (12,5%), palato duro (12,5%) e região submandibular (12,5%). Os principais fatores prognósticos encontrados foram: morfologia celular fusiforme, ulceração, espessura de tumor de 4 a 8,3 mm, nível de Prasad et al. (2004), II e III; nível de Clark IV e V, índice mitótico de 1 a 8, infiltrado linfocitário intratumoral, infiltrado inflamatório peritumoral, formação de satélites microscópicos e linfonodos acometidos. Conclusões: O melanoma maligno mucoso primário de cabeça e pescoço é uma enfermidade pouco comum no Hospital de Câncer de Pernambuco. Não se constatou diferença de acometimento entre gêneros. As faixas etárias de maior ocorrência localizam-se após a 5ª década de vida. A região anatômica mais acometida foi o lábio superior. Houve maior frequência das características associadas a um pior prognóstico, tais como presença de ulceração, nível de invasão III de Prasad, níveis IV e V de Clark e profundidade de invasão de 8,0 a 8,3 mm.
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Lage, Denise 1979. "Doenças liquenoides da pele e mucosa oral = análise histológica e imuno-histoquímica = Lichenoid diseases of the skin and oral mucosa : histological and immunohistochemical analysis." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312892.
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Orientador: Maria Letícia CintraTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: O líquen plano (LP) pode afetar a pele e/ou as mucosas. Histologicamente apresenta infiltrado linfo-histiocitário na junção epitélio-tecido conjuntivo e apoptose de células epiteliais basais. No LP oral (LPO), ocorre erosão frequente pela maior intensidade da necrose. O LP cutâneo (LPC) e o LPO apresentam características histopatológicas similares, mas o curso clínico é diverso. O LPC costuma ter seu curso limitado, enquanto o LPO é frequentemente recidivante. A erupção liquenoide a droga (ELD) desenvolve-se após semanas da ingestão do medicamento e a resolução do quadro é lenta após a interrupção, dificultando o diagnóstico diferencial com o LP idiopático. Os achados clínicos e histológicos podem ser indistinguíveis daqueles do LP, mas a patogênese da ELD não é conhecida. Diferenças locais no sistema imune da mucosa oral e pele poderiam explicar a diversidade no comportamento clínico do LP. Quanto à ELD, há poucas publicações sobre as alterações imunes que atuam no seu desenvolvimento. A citotoxidade celular é mediada, dentre outros mecanismos, por grânulos contendo granzima B e perforina, produzidos por linfócitos T citotóxicos e células natural killers. Com o objetivo de estudar a citotoxicidade celular na patogênese destas doenças, foram analisadas 29 amostras de LPO, 16 de LPC e 6 de ELD. Os cortes foram corados pela H&E e técnica de imuno-histoquímica, para a demonstração de linfócitos CD4 e CD8, macrófagos HAM 56+ e MAC 387+, granzima B, perforina e ICAM-1. As amostras de LPO apresentaram maior densidade de células granzima B+ e perforina+, em comparação às do LPC. Nos dois grupos de doenças, quanto maior era o número de células perforina+, maior era o de células granzima B+. Maior número de células CD4-positivas foi encontrado nas lesões ativas, quando comparado com o das regressivas, no LPO, mas não no LPC. À comparação entre o LPC e a ELD, quanto maior o número de células CD8-positivas, maior era o número de células que expressavam a perforina no grupo LPC. Quanto maiores eram os valores da granzima B, maiores os da perforina, no grupo LPC. Quanto maiores eram os valores da granzima B, maiores os de células apoptóticas agregadas, no grupo da ELD. Nas amostras do LPC, quanto maiores os valores das células T, maiores os dos macrófagos HAM56-positivos e vice-versa. Nas amostras da ELD, foi encontrada correlação negativa entre o número de células T e o de histiocitos jovens (MAC 387+). Havia correlação positiva entre o número de células T e o de células CD8, no grupo da ELD. O mesmo não ocorreu, no grupo do LPC. Concluindo, a expressão aumentada dos grânulos citotóxicos no LPO pode estar associada à maior gravidade da doença na mucosa. Os resultados favorecem um papel mais importante da granzima B e linfócitos TCD8+, no mecanismo patogenético da ELD, comparativamente com o da perforina, de maior importância no LPC. É possível que a ação da granzima B esteja ligada ao número abundante de clusters encontrado na ELD. Embora o LPC e a ELD apresentem semelhanças clínicas e histológicas, a etiopatogênese parece ser distinta
Abstract: Lichen planus (LP) can affect the skin and/or mucous membranes. Histologically it presents lymphohistiocytic infiltrate in the epithelium-connective tissue junction and apoptosis of basal epithelial cells. In oral LP (OLP), erosion occurs frequently by higher intensity of necrosis. Cutaneous LP (CLP) and OLP present similar histopathological features, but the clinical course is diverse. Spontaneous remission is common in CLP, but OLP follows a prolonged course, with periods of remission and relapse. Lichenoid drug eruption (LDE) develops after weeks of drug intake and the resolution of lesions is slow after drug discontinuation, hampering the differential diagnosis with (idiopathic) LP. Clinical and histological findings of LDE may be indistinguishable from those of LP, but LDE pathogenesis is poorly understood Local differences in the immune system of the skin and oral mucosa could explain the diversity in the clinical behavior of CLP and OLP. Regarding LDE, there are few publications on the immune changes that act in its development. Cellular cytotoxicity is mediated, among other mechanisms, by granules containing perforin and granzyme B, produced by cytotoxic T lymphocytes and NK cells. In order to study cellular cytotoxicity in the pathogenesis of these diseases, we analyzed 29 samples of OLP, 16 of CLP and 6 of LDE. The sections were stained for H&E and immunohistochemically targeted with CD4, CD8, HAM 56, MAC387, granzyme B, perforin and ICAM-1. OLP specimens exhibited higher density of cytotoxic granules (perforin and granzyme B) when compared with CLP. In both groups of diseases, the greater the number of perforin+ cells, the greater was the number of granzyme B+ cells. Increased number of CD4+ cells was found in active lesions as compared with the regressive ones in OLP but not in the CLP. The comparison between CLP and LDE revealed that the greater the number of CD8+ cells, the greater the number of cells expressing perforin in CLP group. The higher were the values of granzyme B, the higher the perforin values in the CLP group; the higher were the values of granzyme B, the higher the number of clusters of apoptotic cells in the LDE group. Within CLP group, the higher were the values of T cells, the greater the number of HAM56+ macrophages and vice versa. In LDE samples, negative correlation was found between the number of T cells and young histiocytes (MAC 387+). There was a positive correlation between the number of T cells and CD8 cells in LDE group, but not in CLP group. Concluding, increased expression of cytotoxic granules in OLP may be associated with greater mucosa severity. The results favor a greater role of granzyme B and CD8+ lymphocytes in the pathogenic mechanism of LDE, when compared with perforin, of greater importance in CLP. It is possible that the action of granzyme B is connected to the abundant number of clusters found in LDE. Although CLP and LDE present clinical and histological similarities, the etiopathogenesis appears to be distinct
Doutorado
Anatomia Patologica
Doutora em Ciências Médicas
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Healy, Claire Marie. "Recurrent oral ulceration : in vivo and in vitro studies." Thesis, Queen Mary, University of London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250670.
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Board, Davies Emma. "The antibacterial properties of oral mucosa lamina propria-progenitor cells." Thesis, Cardiff University, 2016. http://orca.cf.ac.uk/91227/.
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Despite the rich oral microflora, infections within the oral cavity are rare. Rapid wound healing within the oral mucosa occurs, potentially due to the presence of oral mucosa lamina propria-progenitor cells (OMLP-PCs). OMLP-PCs are a novel population of multipotent cells known to possess immunosuppressive properties,through contact-independent mediated mechanisms. Many immunomodulatory soluble factors are also documented to have dual functions as antimicrobials; leading to the hypothesis that OMLP-PCs possess antibacterial properties in addition to their published immunoregulatory actions. The aim of this study was to investigate the antibacterial properties of OMLP-PCs and to define the mechanisms of action. A further aim of this study was to determine whether the antibacterial potential of OMLP-PCs was affected during disease, specifically Graft Versus Host Disease (GVHD). The antibacterial properties of OMLP-PCs were compared between cells isolated from healthy donors and patients with oral chronic GVHD. During this study it was determined that OMLP-PCs possess constitutive antibacterial properties against Gram positive and Gram negative bacteria which are mediated through the release of soluble factors. LL37 and Indoleamine 2,3-Dioxygenase are known to mediated the antibacterial properties of bone marrow-mesenchymal stem cells, however this study determined that these factors did not play a role in the OMLP-PCs antibacterial effects. It was established that osteoprotegerin, haptoglobin and prostaglandin E2 in part mediate the antibacterial effects of OMLP-PCs. For the first time, direct antibacterial properties of osteoprotegerin were demonstrated against Gram positive bacteria. Furthermore, OMLP-PCs isolated from GVHD patients did not display antibacterial properties. It was further established that the secretion of innate cell chemoattractants was dysregulated in OMLP-PCs isolated from GVHD patients compared to healthy controls. This finding demonstrates that during GVHD, the oral mucosa is unable to regulate the oral microflora and sufficiently recruit innate immune cells during infection.
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Silva, Rosiane Maria da 1979. "Diferentes padrões de infiltrado celular e citocinas distinguem formas clínicas da paracoccidioidomicose." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308980.
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Orientador: Maria Heloísa de Souza Lima BlottaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: A paracoccidioidomicose (PCM), micose sistêmica causada pelo fungo Paracoccidioides brasiliensis, apresenta um largo espectro de manifestações clínicas e imunológicas, variando de formas benignas e localizadas a quadros graves e disseminados. A forma crônica ou adulta (FA) se caracteriza por lesões localizadas com acometimento da pele, mucosa oral (MO) e pulmões; enquanto a forma aguda ou juvenil (FJ) é mais grave e disseminada com acometimento do fígado, baço e linfonodos (LNs). Nós examinamos a resposta inflamatória/imunológica em lesões de pacientes com formas clínicas distintas da PCM. Foram analisados a expressão de citocinas e o fenótipo das células presentes nos infiltrados de biópsias de mucosa oral de 15 pacientes com a forma adulta e de linfonodos de 15 pacientes com a forma juvenil da PCM por imuno-histoquímica (IHQ) e qPCR. Anticorpos monoclonais ou policlonais foram utilizados para caracterizar o infiltrado celular (CD68, CD15, CD3, CD8, CD56 e FOXP3), citocinas...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital
Abstract: Paracoccidioidomycosis (PCM), a systemic disease caused by the fungus P. brasiliensis, presents with a wide spectrum of clinical and immunological manifestations, varying from benign and localized forms to severe and disseminated forms. The chronic or adult form (AF) is characterized by localized lesions with involvement of the skin, oral mucosa (OM) and lungs. While the acute or juvenile form (JF) is more severe and presents disseminated widespread with the involvement of the liver, spleen and lymph nodes (LNs). In order to examine the inflammatory/immune response in lesions of patients with different clinical forms of PCM, we analyzed the expression of cytokines as well as the phenotype of the cells in the inflammatory infiltrate. Paraffin-embedded OM biopsies from 15 patients with the localized AF of PCM and LN from 15 patients with the JF of PCM were analyzed by immunohistochemistry (IHC) and qPCR. Monoclonal or polyclonal antibodies were used to characterize the cellular infiltrate (CD68, CD15, CD3, CD8, CD56, and FOXP3), cytokines...Note: The complete abstract is available with the full electronic document
Mestrado
Ciencias Biomedicas
Mestre em Ciências Médicas
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Franz-Montan, Michelle 1982. "Avaliação da eficacia anestesica da ropivacaina a 1% encapsulada em lipossomas, em anestesia topica em odontologia." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288955.
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Orientadores: Francisco Carlos Groppo, Eneida de PaulaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O objetivo deste estudo cruzado e cego foi avaliar a eficácia da anestesia tópica de ropivacaína a 1% (forma lipossomada ou não) em Odontologia. Trinta voluntários foram submetidos à anestesia tópica com quatro anestésicos, em oito sessões (tratamentos) determinadas aleatoriamente: 20mg de gel de ropivacaína a 1% -Ropi-20, 60mg de gel de ropivacaína a 1% -Ropi-60, 20mg de gel de ropivacaína a 1% encapsulada em lipossomas -RopiLipo-20, 60mg de gel de ropivacaína a 1% encapsulada em lipossomas -RopiLipo-60, 20mg da mistura eutética de lidocaína a 2.5% e prilocaína a 2.5% (EMLA cream AstraZeneca®) - EMLA-20, 60mg de EMLA cream -EMLA-60, 20mg de gel de benzocaína a 20% (Benzotop® DFL) -Benzo-20 e 60mg de gel de benzocaína a 20% -Benzo-60. Cada tratamento foi aplicado no fundo de sulco da região de canino superior direito durante dois minutos; o intervalo entre os tratamentos foi de uma semana. Foram avaliadas a anestesia pulpar, por meio de estímulo elétrico (pulp tester); a dor durante a punção por meio das escalas analógica visual (EAV) e de 11 pontos em caixa (E11); e a anestesia em tecido mole por meio de estímulo físico. A influência do anestésico na resposta pulpar foi avaliada durante 20 minutos após a aplicação do anestésico tópico. Não houve diferença estatisticamente significante entre os tratamentos com relação às escalas EAV e E11 (p>0.05). A duração da anestesia em tecido mole com o EMLA-60 e RopiLipo-60 foi maior (p<0.05) do que com os outros tratamentos. No entanto, entre EMLA-60 e RopiLipo-60 não houve diferença estatisticamente significante (p>0.05). Nenhum dos tratamentos avaliados exerceu efeito anestésico sobre o tecido pulpar. Assim, a ropivacaína a 1% encapsulada ou não em lipossomas apresentaram eficácia semelhante em reduzir a dor à punção em comparação aos outros anestésicos avaliados, no entanto, nas condições avaliadas, nenhum dos anestésicos exerceu influência sobre a resposta pulpar
Abstract: The objective of this blind and crossover study was to evaluate the efficacy of 1% ropivacaine (liposomal and nonliposomal) for topical anesthesia in Dentistry. Thirty healthy volunteers randomly received the following treatments in eight sessions: 20mg of 1% ropivacaine -Ropi-20, 60mg of 1% ropivacaine -Ropi-60, 20mg of liposome encapsulated 1% ropivacaine -RopiLipo-20, 60mg of liposome encapsulated 1% ropivacaine -RopiLipo-60, 20mg of the eutectic mixture of 2.5% lidocaine and 2.5% prilocaine (EMLA cream AstraZeneca®) -EMLA-20, 60mg of EMLA cream -EMLA-60, 20mg of 20% benzocaine gel (Benzotop® DFL) -Benzo-20 and 60mg of 20% benzocaine gel -Benzo-60. At different sessions each treatment was applied in the maxillary-buccal fold of the right canine for two minutes; one week of interval between treatments. Pulpal anesthesia was evaluated using an electrical pulp tester; pain during needle insertion by using a visual analogue scale (VAS) and 11-point box scale (BS-11) and soft tissue anesthesia by using a pinprick test. The influence on pulpal response was assessed for 20 minutes after topical application. There were no statistically significant differences among treatments considering VAS and BS-11 scores (p=0.177 and p=0.179, respectively). RopiLipo-60 and EMLA-60 provided a statistically significant longer duration of topical anesthesia than the other topical agents (p<0.05), however, RopiLipo-60 was not significantly different from EMLA-60 (p>0.05). There was no influence on the pulpal response provided by the topical anesthetics evaluated. Liposome and nonliposomal ropivacaine gel at 1% concentration showed similar efficacy for topical anesthesia in oral mucosa when compared to the other topical anesthetics. However, none of the topical anesthetics evaluated were effective in inducing pulpal anesthesia under the evaluated conditions
Mestrado
Farmacologia, Anestesiologia e Terapeutica
Mestre em Odontologia
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Baumgart, Cristina da Silva. "Influência da condição bucal, hábitos e fatores socioedemográficos no padrão citológico da mucosa bucal normal." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/152972.
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Proposição: Avaliar a associação entre as condições de saúde bucal, fatores comportamentais e sociodemográficos e o padrão citopatológico da mucosa bucal de homens adultos. Correlacionando o padrão citopatológico quantitativo e qualitativo com as variáveis bucais e sociodemográficas. Materiais e Métodos: A partir de dois esfregaços, um da borda da língua e outro do assoalho bucal de 117 homens com mais de 25 anos, foram quantificadas cem células de cada. As células foram coradas pelo método Papanicolaou modificado e classificadas em: Escamas, células superficiais com núcleo, células intermediárias e células parabasais. Variáveis sociodemográficas e comportamentais foram coletadas a partir de um questionário estruturado. Índice CPO-D e uso de próteses foram registradas a partir de um exame clínico intra-oral.As análises foram conduzidas utilizando o pacote estatístico Stata versão 10. O indivíduo foi considerado a unidade analítica. O nível de significância foi estabelecido em 5%. Resultados: No total das células na borda da língua, 75% eram intermediárias, 20% superficiais com núcleo e 5% escamas, sendo que células parabasais foram raramente observadas. Não foram observadas diferenças significativas para todas as variáveis em estudo em todos os tipos celulares, com exceção da ingestão de bebidas alcoólicas.Observou-se percentual significativamente maior de escamas e células superficiais com núcleo nos indivíduos que ingeriam de álcool comparados aos que não ingerem. Para as células intermediárias, o percentual foi significativamente menor nos indivíduos que bebem comparados aos que não bebem. Um padrão semelhante de distribuição celular foi observado no assoalho de boca. O consumo de álcool foi o único fator comportamental que influenciou significativamente no padrão citológico da mucosa bucal dos indivíduos analisados nos modelos multivariados. Conclusões: Foi encontrada associação entre um dos fatores comportamentais (o álcool) e o padrão citopatológico da mucosa bucal de homens adultos. As demais variáveis estudadas não apresentaram associação significativa com o padrão de descamação da mucosa bucal normal com a técnica utilizada.Proposition: To evaluate the association between oral health status, socio- demographic and behavioral factors and standard cytology of the oral mucosa of adult men. Correlating the standard cytopathology quantitative and qualitative with oral and sociodemographic variables. Materials and Methods: From two smears, one edge of the tongue and other oral floor of 117 men over 25 years old, were quantified hundred cells each. Cells were stained with modified Papanicolaou method and classified into: Scales, superficial cells with nuclei, intermediate and parabasal cells. Sociodemographic and behavioral variables were collected from a structured questionnaire. DMFT index and use of prostheses were recorded from a clinical intra - oral.As analyzes were conducted using Stata version 10. The individual was considered the analytical unit. The level of significance was set at 5 %. Results: In total cells at the edge of the tongue 75% were intermediate, 20% core surface scales and 5%, and parabasal cells were rarely observed. No significant differences were observed for all study variables in all cell types, with the exception of beverage intake alcoólicas.Observou is significantly greater percentage of scales and superficial cells in the core subjects ingestores alcohol compared to those who do not drink. For intermediate cells, the percentage was significantly lower in individuals who drink compared to those who do not drink. A similar pattern of cell distribution was observed in the floor of mouth. Alcohol consumption was the only factor that significantly influenced the behavioral cytology of the oral mucosa of individuals analyzed in the multivariate models. Conclusions: An association between a behavioral factor (alcohol) and standard cytology of the oral mucosa of adult men. The other variables were not significantly associated with the default scaling of normal oral mucosa with the technique used.
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Kulkarni, Upendra D. "Optimization of porcine buccal mucosa for in vitro evaluation." Scholarly Commons, 2007. https://scholarlycommons.pacific.edu/uop_etds/652.
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Porcine buccal mucosa has been extensively used as in vitro model to study the permeability of drugs and assess their potential to deliver through buccal route. Porcine buccal mucosa is found to be very similar to human oral mucosa in structure and function. However, the in vitro permeation studies across porcine buccal mucosa show high variability which is mostly due to the various experimental and biological variables that are often overlooked while conducting such studies.
The precise nature of the permeability barrier offered by the various tissue layers of buccal mucosa was investigated in this study. It was observed that the permeability of model diffusants decreased significantly with an increase in the connective tissue layer. However, the epithelium offered a stronger barrier to permeation of all diffusants studied at mucosal thickness of up to 500 |tm. The epithelium acted as a stronger barrier for hydrophilic diffusants when compared to lipophilic diffusants.
It was also observed that the permeability of model diffusants was significantly higher in the region behind lip when compared to the middle cheek region which is due to lower epithelial thickness in that region. Porcine buccal mucosa retained its integrity in Kreb's bicarbonate Ringer solution at 4 °C for 24 hours and many other storage conditions resulted in loss of epithelial integrity. Separation of epithelium from the underlying connective tissue by heat treatment, did not adversely affect its permeability and integrity characteristics.
Influence of experimental temperature on the permeability of model compounds across porcine buccal mucosa was also investigated in vitro. An exponential relationship was observed between the apparent permeability and temperature. It was found that the activation energy of diffusion of the model compounds decreased linearly with increasing distribution coefficients across porcine buccal mucosa. This suggested that the buccal mucosa acted as a stronger barrier for diffusion of hydrophilic diffusants when compared to the lipophilic diffusants.
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Ferrari, Junia Carolina Linhares. "Utilização do laser de baixa densidade (LILT) para tratamento da mucosite induzida em hamsters : comparação clínica e histopatológica entre parâmetros de irradiação /." Araraquara : [s.n.], 2009. http://hdl.handle.net/11449/104221.
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Orientador: Fabio Cesar Braga de Abreu e LimaBanca: Rosane Lizarelli
Banca: Marcelo Chaves Donizeti
Banca: Cyneu Pansani
Banca: Elaine Maria Sgavioli Massucato
Resumo: A cavidade oral é alvo freqüente dos efeitos tóxicos dos agentes antineoplásicos por apresentar tecidos com rápida divisão celular, comparável à dos tumores malignos. Uma das complicações orais mais freqüentes da quimioterapia é a mucosite, uma alteração de caráter inflamatório para a qual ainda não existe tratamento definido. Considerando-se a relevância clínica da mucosite, é importante encontrar meios para tratá-la. O presente estudo teve como objetivo avaliar o efeito do LILT na redução da incidência e da severidade da mucosite induzida em hamsters. O quimioterápico 5-FU foi aplicado em 60 animais (distribuídos em 5 grupos) nos dias 0 e 2 do experimento, nas doses de 90 e 60 mL/Kg de peso, respectivamente. Para simular o efeito de uma irritação crônica, as mucosas jugais dos animais foram escarificadas nos dias 3 e 4. Aplicou-se o LILT em 3 pontos da mucosa jugal direita dos animais dos Grupos I, II, III e IV em dias alternados. Os animais do Grupo V não receberam tratamento. Nos dias 8 e 12 do experimento, as mucosas de 6 animais por grupo foram removidas para avaliação histopatológica. A partir de fotografias tiradas diariamente, a mucosite foi classificada clinicamente. O teste de Mann-Whitney demonstrou haver diferenças estatisticamente significantes (p<0,05) entre os grupos tratados com laser e o grupo não tratado quando se comparou, clinica e histopatologicamente, a intensidade da mucosite induzida. Concluiu-se que a aplicação do LILT, nos parâmetros determinados para este estudo, promoveu a redução da severidade da mucosite oral e acelerou a cura das lesões, parecendo haver maior melhora nos animais que receberam 12 e 72 J/cm2.
Abstract: Toxic and dose-limiting effects of antineoplastic agents in oral cavity are frequently observed during cancer therapy. The available therapies are not able to destroy tumor cells without causing damage to the rapid dividing cells in normal tissues like the epithelial cells in the oral mucosa. Mucositis is the most debilitating side effect, for which there is no established treatment. Considering the clinical significance of mucositis, it is important to find ways to treat this condition. The present study was conducted to evaluate the LILT effects on reduction of chemotherapy-induced mucositis in hamsters. Mucositis was induced in 60 animals with intraperitoneal injections of 5-fluorouracil (5-FU) on days 0 and 2, associated with cheek pouches scarification on days 3 and 4. Animals were dived in 5 groups and groups I, II, II, IV and V received laser irradiation at three points in right cheek pouch, at alternate days. Group V received no treatment. The cheek pouches of 6 animals in each group were dissected for histophatological examination on days 4 and 12. Daily photographs were taken and mucositis was clinically scored. The nonparametric test of Mann- Whitney showed statistical difference between treated and non treated group (p<0.05). It was concluded that the LILT protocols established for this study reduced the severity of oral mucositis and accelerated the healing process, with better results when 12 and 72 J/cm2 were used.
Doutor
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Rousseau, André. "Cyclin D1 gene amplification and protein overexpression in dysplastic oral mucosa and oral cancer." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0015/MQ53416.pdf.
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OKA, TOHRU, MASARU NAGAYAMA, TOSHIO KANEDA, and MINORU UEDA. "Clinical Evaluation of Reversed Dermis Graft for Reconstruction of Oral Mucosa." Nagoya University School of Medicine, 1986. http://hdl.handle.net/2237/17490.
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Al-Johani, K. "Outcomes of therapy of immunologically-mediated diseases of the oral mucosa." Thesis, University College London (University of London), 2010. http://discovery.ucl.ac.uk/751812/.
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Immune-mediated diseases (IMDs) can give rise to long standing painful oral mucosal disease which adversely affect oral function and perhaps lessens quality of life. The present series of studies, retrospectively determine the clinical presentation and long-term efficacy and safety of treatment of large groups of patients with oral lichen planus, mucous membrane pemphigoid, pemphigus vulgaris and orofacial granulomatosis. These diseases are some of the challenging disorders to be managed by oral medicine specialists. It was found that patients with oral lichen planus (OLP) rarely have extra-oral manifestations of LP. The symptoms of OLP can generally be controlled with topical corticosteroids and/or tacrolimus. While tacrolimus is not notably better than topical corticosteroids for the management of OLP, it does not seem to increase any risk of malignant transformation. Adverse side effects are uncommon with topical corticosteroids, while 21% of patients with OLP may have adverse side effects with tacrolimus, particularly unpleasant taste. In the present cohort of 49 patients with orofacial granulomatosis (OFG) the onset of disease was characterised by facial swelling in 50% and the long-term behaviour of OFG was characterised by the development of further clinical manifestations with most patients developing orofacial swelling and/or intra-oral ulceration. The response of OFG to therapy was typically remitting and although a lessening of soft tissue swelling oral ulceration could generally be achieved with topical and/or systemic therapy. Complete remission of facial swelling occurred in 50% of patients within 3 years of therapy but may be achieved quicker when intra-lesional corticosteroids are used. Spontaneous remission was rare. Significant adverse side effects to therapy were rare. In a cohort of 62 patients, mucous membrane pemphigoid typically manifested as recurrent oral mucosal ulceration and/or desquamative gingivitis and 32.3% patients had some extra-oral involvement. Treatment generally lessened painful symptoms however gingival lesions rarely resolved. Adverse side effects affected 50% of patients; however in the majority of affected individuals these were minor. In a cohort of 40 patients with pemphigus vulgaris the mouth was often the initial site of involvement but other mucocutaneous sites could be affected. Management necessitated topical and systemic therapy. Adverse side effects occurred in 50% patients and were mainly associated with systemic immunosuppressive agents (e.g. azathioprine). The results of this present study indicate that the long-term treatment of IMDs of the oral mucosal are challenging to both the patients and clinicians. While many patients do experience an improvement in their disease status, many do not. The precise impact of IMDs upon the quality of life of affected individuals remains unclear.
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Best, Mark. "Oral mucosa-nanoparticle interactions and uptake pathways in formulation excipient profiling." Thesis, University of Brighton, 2014. https://research.brighton.ac.uk/en/studentTheses/dbad162f-6df6-40d6-ac5d-148d77b5aff8.
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Nanomaterials are generally defined as chemical substances or materials that contain particles with one or more dimensions less tl1an 100 nanometres in size. They may be either engineered or naturally occurring, but have unique properties due to a vastly increased surface area to volume ratio when compared to non-nano (bulk) materials. This provides the potential for the development of a wide range of enhanced formulations with superior efficacy including applications in oral health care .. As the properties of a material change at the nano-scale, there are concerns that the toxicological profile of these materials may also change. Size is only one factor; changes in shape, surface chemistry, chemical composition, porosity and solubility all contribute to the overall biological toxicity profile of a nano-scale ingredient. Established links between the specific properties of a nanomaterial and toxicity are not well understood, leaving an important data gap in the literature. The purpose of this work was to utilise in vitro oral epithelial models for the assessment of safety profiles of nanomaterials for applications in next generation oral care products. Four commercially sourced nanomaterials were analysed, alongside respective bulk counterparts already found within oral care product formulations. These nanomaterials comprised of two nano-zinc oxides (ZnO), silicon dioxide (SiOz), titanium dioxide (TiOz) and hydroxyapatite (Cas(OH)(P04)'). Comprehensive characterisation of each material was carried out using a range of analytical techniques to identify any structure-function relationships in vitro. Initial toxicity screening experiments were conducted using a non-keratinised oral epithelial cell monolayer (H376 cell line) with both cell viability and lysis analysed using MTT and LDH assays respectively. Materials were investigated further using two 3-dimensional tissue models representative of the main tissue types constituting the human oral mucosa: non-keratinised buccal (RHO) and keratinised gingival (GIN-lOO) models. Nanomaterial uptake in the models was investigated using confocal microscopy with a styrl dye (FM 1-43). This led to the development of a novel, high throughput fluorescent assay as a potential method for screening nanoparticle-uptake. Results highlighted the complexities involved with nano-characterisation in biological media using current techniques. A wide variety of particle shapes and sizes were recorded between different nanomaterials, with results being dependent upon the sample preparation steps and specific methods of analyes used. These disparities represent the current challenges experienced by both researchers and regulators of nanotechnology at the present time. ZnO \vas observed to be the most cytotoxic material during monolayer screening, at concentrations exceeding 0.3125% w/v when delivered in protein-free media. Differences between bulk and nanomaterial properties were recorded for all the materials, except for Ti02, but these did not necessarily transfer to effects seen in the more representative 3-D models. Cytotoxicity results from both R1--10 and GIN-lOO models exemplified the disparity between sensitivity of monolayer and the natural stratified tissue structure of human oral mucosa. Keratinised gingival tissue models showed slgnificantly greater durability over the less robust buccal model, in both cytotoxicity assays and IL- lcx cytokine response. Of all materials examined, cellular uptake was only observed for nano-Si02. This was the only material detected trafficking inside the cell using the FM 1-43 styryl dye assay, with confocal data serving to verify the analysis of nanoparticle Internalisation using fluorescence. In conclusion, nanomaterials pose considerable difficulties during formulation and analysis in health care products. The risk of potential uptake and bioaccumulation or translocation to particularly sensitive areas of the body also requires further investigation. Nanomaterials have to be assessed on a case by case basis, and robust/consistent regulatory strategies developed to enable industry to produce and market novel but safe nanoparticle containing formulations. Risks to human health may be less of a hazard when applied to fully functioning healthy human tissue, especially in comparison to existing bulk material effects and current, accepted irritant ingredients (e.g. Sodium lauryl sulphate).
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Rivarola, de Gutiérrez Emilce M. "Tratamiento con antioxidantes locales de patologías inflamatorias de la mucosa oral." Doctoral thesis, Universidad Nacional de Cuyo. Facultad de Ciencias Médicas, 2015. http://bdigital.uncu.edu.ar/10031.
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El estrés oxidativo es causado por un desequilibrio entre la producción de especies reactivas del oxígeno (ROS) generadas en el metabolismo normal, y en mayor grado en la inflamación; y la capacidad de los tejidos para neutralizar los reactivos intermedios o reparar el daño resultante.
En las patologías de la mucosa bucal, que incluye este trabajo, se ha demostrado la presencia de estrés oxidativo en la fisiopatogenia. Las patologías evaluadas son: liquen plano bucal (LPB), síndrome de ardor bucal, mucositis y aftas. El grupo de los pacientes con LPB fue subdividido en: formas erosivas incluyendo al LPB erosivo y a la forma ampollar y formas no erosivas, abarcando esta última categoría: LPB reticular, queratósico, atrófico y pigmentario. Se estudió el efecto de un tratamiento local con antioxidantes extraídos de las uvas, antocianinas, comparándolo con los tratamientos habitualesFil: Rivarola de Gutiérrez, Emilce M.. Universidad Nacional de Cuyo. Facultad de Odontología.
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Villouta, Bascuñán María-Renée. "Frecuencia y expresión clínica de lesiones de mucosa oral en pacientes del Servicio de Diagnóstico." Tesis, Universidad de Chile, 2010. http://repositorio.uchile.cl/handle/2250/134422.
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Trabajo de Investigación Requisito para optar al Título de Cirujano DentistaAutor no autoriza el acceso a texto completo de su documento
El objetivo de este estudio fue determinar las patologías de la mucosa oral más frecuentes entre los pacientes que acuden al Servicio de Diagnóstico de la Facultad de Odontología de la Universidad de Chile, y su asociación con factores demográficos. Se llevó a cabo un estudio descriptivo retrospectivo donde se analizaron las fichas clínicas y registros de 14.479 pacientes que acudieron al servicio entre Enero de 2001 y Diciembre de 2008, registrándose, edad, sexo, diagnóstico, localización anatómica y expresión clínica de las lesiones. El 3.40% (n=493) de los pacientes presentaron patologías de la mucosa oral, de ellos 65.11% mujeres y 34.88% hombres. Las lesiones más frecuentes fueron las reaccionales (23.32%), la localización anatómica más frecuente fue la cara interna de la mejilla (19.91%) y lengua (19.49%). La expresión clínica más frecuente fue el tumor (34.92%). Las diez lesiones de mucosa oral más frecuentes fueron el pseudofibroma (15.41%), Liquen plano (8.52%), UROs (8.11%), Candidiasis (7.10%), Granuloma telangectásico (6.50%), CEC (6.90%), Penfigoide mucoso (5.07%), Papiloma (4.26%), Leucoplasia (3.66%) y Queilitis Actínica (2.84%). Los resultados del presente estudio coinciden en gran medida con los reportados por la literatura internacional.
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Kim, Yeon Jung [UNESP]. "Avaliação do efeito da Artin M no processo de repação em mucosa mastigatória." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/104723.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Artin M é uma lectina purificada de sementes de Artocarpus heterophyllus que, recentemente, mostrou-se capaz depromover aceleração da cicatrização de lesões por queimadura de pele ou por abrasão da córnea em ratos e coelhos. O objetivo desta tese foi avaliar os efeitos da Artin M no processo de reparação da mucosa mastigatória bucal, por meio de modelos de estudo in vivo. Primeiro estudo: Três feridas cirúrgicas circulares de 6 mm de diâmetro foram criadas na mucosa palatina de 20 cães, e divididas aleatoriamente em 3 grupos de acordo com os tratamentos: C – controle (não tratados), A - Artin M, V - veículo. Quatro animais de cada grupo foram sacrificados após 2, 4, 7, 14 e 21 dias póstratamento e suas maxilas analisadas clinicamente quanto ao padrão de cicatrização, seguido de análise histológica, imuno-histoquímica para antígeno nuclear de proliferação celular (PCNA) e atividade de mieloperoxidase. Clinicamente, o grupo A demonstrou melhor cicatrização em todos os períodos quando comparada aos outros grupos (p<0,05). A análise histológica mostrou ter havido no grupo A maior estimulação na produção de fibras colágenas, maturação do tecido de granulação e organização do epitélio. A imunolocalização de PCNA mostrou uma maior tendência na proliferação celular em lesões do grupo A principalmente nos dias iniciais (p<0,05). O influxo de neutrófilos mostrou-se estatisticamente aumentado no grupo A quando comparado aos outros grupos nos dias 2 e 4 (p<0,05). Conclui-se que a Artin M promoveu aceleração na reparação das feridas na mucosa mastigatória em cães, via recrutamento de neutrófilos e indução da proliferação celular. É bem conhecido o envolvimento de mediadores biológicos como citocinas...
Artin M, lectin from Artocarpus heterophyllus seeds, has been demonstrated to stimulate recruitment and activation of neutrophil and mast cells. Furthermore, it has been shown to accelerate the process of wound healing on burn injuries and corneal epithelial lesions in rats and rabbits, respectively. The aim of this study was to evaluate the effects of Artin M on wound healing in palatal mucosa in two animal models. Study 1: Three wounds of 6 mm full thickness were surgically created in the hard palate mucosa of twenty dogs. The wounds of each animal were randomly divided into three groups according to one of the treatment assigned: C– Control (nontreated), A- Artin M gel, and V– Vehicle (carboxymethylcellulose 3% gel). Four animals per group were sacrificed at 2, 4, 7, 14 and 21 days post-surgery. Before sacrifice, wounded areas were photographed and scored for macroscopic healing evaluation. Afterwards, maxillary tissue were harvested and divided to perform histological analysis, immunohistochemical staining against Proliferating Cell Nuclear Antigen (PCNA) and measurement of myeloperoxidase activity. Clinical analysis showed that wound closure was accelerated in group A in comparison to other groups in all periods. Histological features showed enhanced reepithelization and collagen fiber formation resulting in faster maturation of granular tissue in group A compared to the other groups, by day 14. Artin M treatment significantly induced cells proliferation at day 2 and 4 (p<0.05). Neutrophils infiltration in the group A was significantly higher than in the other groups at days 2 and 4 (p<0.05). The results suggest that Artin M gel may potentially facilitate wound healing on palatal mucosa via the recruitment of neutrophils and promotion of cells proliferation. It is well known that cytokines and growth... (Complete abstract click electronic access below)
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Ribeiro, Mariana Goveia Melo. "Detecção e genotipagem do papilomavírus humano (HPV) em mucosa oral de pacientes do Estado de Sergipe, Brasil." Universidade Federal de Sergipe, 2014. https://ri.ufs.br/handle/riufs/3253.
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Infection with Human Papillomavirus (HPV) is the sexually transmitted viral disease most prevalent in world. The infections can range from asymptomatic establishment to induction of squamous cell carcinomas. It has been discussed the correlation of HPV infection and the development and/or aggravation of lesions in the oral mucosa. The aim of this study was to evaluate the occurrence of HPV and its genotypes in patients with oral lesions and in healthy oral mucosa of users and non-users of drugs in the state of Sergipe, Brazil. Thirty-nine patients aged 2 to 83 years with clinically detectable lesions in the oral mucosa and 106 patients with healthy oral mucosa between 11 and 79 years were evaluated. Samples were collected by exfoliating the oral mucosa. For quality control of DNA extraction beta-globin PCR was performed. HPV DNA was detected using primers MY09/MY11 and GP5+/GP6+. Genotyping was performed by multiplex-PCR with specific primers for HPV types 6, 16 and 18. Our study detected the virus in all types of lesions evaluated. The occurrence of HPV was 76.92% (30/39) in patients with oral lesions. The most common virus type was HPV-6 in 56.67% (17/30), followed by HPV-18 in 26.67% (8/30) and HPV-16 in 6.67% (2/30). Positive results were found in 83.02% (88/106) of patients with healthy oral mucosa. The most common virus type was HPV-6 in 45.45% (40/88), followed by HPV-18 in 35.23% (31/88) and HPV-16 in 4.54% (4/88). Between multiple drug users 86.67% (52/60) were positive and multiple HPV infections were identified in 23.08% (12/52). At |non-users| the occurrence was 78.26% (36/46). A high occurrence of HPV was found in the study, both in oral lesions and in healthy mucosa. Rates of HPV detection in the oral cavity vary markedly in the world and make the relationship between HPV and oral carcinogenesis still controversial. Additional studies to evaluate the role of human papillomavirus in the development of lesions in the oral mucosa are necessary. There are few data available on the frequency of oral HPV infection in Brazilian population and especially among drug users. Other studies on HPV prevalence among drug users are needed for a better understanding of their exposure to the virus and for the development of prevention strategies.A infecção pelo Papilomavírus Humano (HPV) é a doença viral sexualmente transmissível mais prevalente no mundo. Suas infecções podem variar de assintomáticas à indução de Carcinomas de Células Escamosas. Entre os agentes infecciosos associados ao câncer oral, tem-se discutido a correlação da infecção por HPV em mucosa oral e o desenvolvimento e/ou agravamento das lesões. O objetivo deste estudo foi avaliar a ocorrência do HPV e seus genótipos em pacientes com lesão oral e em mucosa oral saudável de usuários e não-usuários de drogas no estado de Sergipe, Brasil. Foram avaliados 39 pacientes com idade entre 2 e 83 anos, com lesões clinicamente detectáveis na mucosa oral e 106 pacientes com mucosa oral saudável entre 11 e 79 anos. As amostras foram coletadas por esfoliação da mucosa oral. Para o controle de qualidade da extração de DNA foi utilizado PCR para beta-globina. DNA-HPV foi detectado utilizando primers MY09/MY11 e GP5+/GP6+. A genotipagem foi realizada através de multiplex-PCR com primers específicos para os tipos virais 6, 16 e 18. Nosso estudo detectou DNA-HPV em todos os tipos de lesões avaliadas. A prevalência do HPV foi de 76,92% (30/39) nos pacientes com lesões orais. O tipo viral mais frequente foi o HPV-6, presente em 56,67% (17/30), seguido do HPV-18 em 26,67% (8/30) e do HPV-16 em 6,67% (2/30). DNA-HPV foi encontrado em 83,02% (88/106) dos pacientes com mucosa oral sadia. O tipo viral mais frequente foi o HPV-6, presente em 45,45% (40/88), seguido do HPV-18 em 35,23% (31/88) e do HPV-16 em 4,54% (4/88). Entre usuários de múltiplas drogas 86,67% (52/60) foram positivos e infecções múltiplas por mais de um tipo viral foram identificadas em 23,08% (12/52) dos indivíduos. Entre os "não usuários" a taxa de infecção pelo HPV foi de 78,26% (36/46). Desta forma, foi verificada uma alta ocorrência do HPV em nosso estudo, tanto em lesões orais quanto em mucosas saudáveis. As taxas de detecção do vírus em cavidade oral variam acentuadamente no mundo e tornam a relação do HPV com o processo de carcinogênese oral ainda controversa. Isso faz necessária a realização de estudos adicionais que avaliem o papel do Papilomavírus Humano no desenvolvimento de lesões na mucosa oral. Há poucos dados disponíveis sobre a frequência de infecção oral por HPV na população brasileira e especialmente entre usuários de drogas. Novos estudos sobre a prevalência do HPV entre usuários de drogas são necessários para melhor compreensão da sua exposição ao vírus e o desenvolvimento de estratégias de prevenção.
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Lima, Marina de Deus Moura de. "Correlação entre a presença do HPV na boca e no colo uterino de pacientes com sorologia positiva e negativa para o HIV." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-29092009-091212/.
Full textAbstract:
A infecção genital pelo papilomavírus humano (HPV) corresponde a uma das doenças sexualmente transmissíveis mais frequentes no mundo. Uma preocupação dos pacientes que apresentam HPV na região anogenital diz respeito à possibilidade de disseminação desse vírus para outras partes do corpo. O objetivo geral desse estudo foi avaliar a possível correlação existente entre infecções pelo HPV na mucosa oral e no colo uterino em mulheres com sorologias positiva e negativa para o HIV. Pretendeu-se, também, identificar variáveis clínicas, demográficas e laboratoriais associadas à infecção oral pelo HPV. A amostra foi constituída por 200 pacientes do gênero feminino, sendo 100 com sorologia positiva para HIV (grupo 1) e 100 com sorologia negativa para HIV (grupo 2). As pacientes foram incluídas consecutivamente no Centro de Referência e Treinamento em DST-AIDS entre abril de 2008 a maio de 2009. Todas as pacientes assinaram um Termo de Consentimento Livre e Esclarecido e responderam a uma ficha com questionamentos sobre hábitos e comportamento sexual. Além disso, tiveram as cavidades oral e ginecológica examinadas, sendo que células superficiais de ambos os locais foram coletadas e avaliadas pela captura híbrida 2 e pela citologia em base líquida. Para comparação de variáveis qualitativas, como freqüências e proporções, foi utilizado o teste de qui-quadrado ou exato de Fisher, se necessário. Para comparação de dados quantitativos, foram utilizados os testes de Mann-Whitney ou t de Student. A análise multivariada foi executada utilizando-se o teste de regressão logística, sendo que o valor de significância estatística estabelecido foi de 5% (p<0,05). O DNA do HPV foi detectado nas amostras cervicais de 41 (41%) pacientes HIV+ e de 45 (45%) HIV- (p=0.67). Nas amostras da cavidade oral, o DNA do HPV foi observado em 11 mulheres do G1 (HIV+) e em 2 mulheres do G2 (HIV-) (OR=6,06; 95%IC=1,31-28,07; p=0,02). Os subtipos oncogênicos foram prevalentes em ambos os grupos, sendo que não foi observada diferença entre os grupos (p=0.87). Nenhuma paciente apresentou lesão macroscópica oral relacionada ao HPV, sendo que 15 (15%) mulheres do G1 (HIV+) e 17 (17%) do G2 (HIV-) apresentaram lesão macroscópica na região genital (p=0.2129). Com os resultados obtidos pôde-se concluir que nesta população não houve correlação entre a infecção pelo HPV nas mucosas oral e cervical. Além disso, as pacientes HIV+ apresentaram maior prevalência de infecção pelo DNA-HPV na boca em comparação às pacientes HIV-.Human papillomavirus (HPV) is one of the most prevalent sexually transmitted viruses worldwide with both oral and genital manifestations. The high prevalence of HPV infection among HIV + individuals provides an opportunity to elucidate the relationship between oral and cervical HPV-infection in this group of subjects. The aim of this study is to evaluate the possible association between oral and cervical infections in HIV-positive and negative patients. One hundred HIV+ (group 1) and 100 HIV- (group 2) women were recruited consecutively from a gynecologic clinic between April 2008 and May 2009. All subjects were given a cervical and oral examination. Cytological samples were evaluated by the hybrid capture 2 technique from oral and cervical scrapings. Statistical analysis was performed using chi-square test and p values < 0.05 were considered significant. HPV-DNA was detected in cervical scrapings from 41 (41%) HIV-positive subjects and from 45 (45%) HIVnegative subjects (p=0.67). In oral samples, HPV-DNA was observed in 11 subjects from group 1 and in 2 subjects from group 2 (p=0.02). High-risk HPV subtypes were prevalent in both groups and no difference between the groups was detected (p=0.87). No subject showed macroscopic oral HPV-related lesion, whereas 15 (15.00%) from group 1, and 17 (17.00%) from group 2, presented with macroscopic genital lesion (p=0.2129). HPV-DNA was more frequent in oral mucosa of HIV+ patients than HIV- (p=0,018). There was no association between oral and cervical HPV infection in HIV+ and HIV- patients. Presence of cervical lesion was not associated with oral lesion.
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Johannesson, Ulrika. "Combined oral contraceptives - impact on the vulvar vestibular mucosa and pain mechanisms /." Stockholm : Karolinska institutet, 2007. http://diss.kib.ki.se/2007/978-91-7357-279-8/.
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Napier, Seamus Stephen Mary. "Histopathological predictors of behaviour of potentially malignant lesions of the oral mucosa." Thesis, Queen's University Belfast, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326288.
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Santiago, Andrezza Fernanda. "Efeitos do envelhecimento na mucosa intestinal: indução e declínio da tolerância oral." Universidade Federal de Minas Gerais, 2011. http://hdl.handle.net/1843/UCSD-8EKKMK.
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Aging is reported to be associated with a decline in oral tolerance induction, an immunological phenomenon initiated at the intestinal mucosal surface. Mice become less susceptible with 24 weeks of age and totally refractory with 70 weeks of age. At this age, they can still be rendered tolerant but only by a regimen of continuous feeding. Herein, we examined the effect of aging in T cells and cytokines at the intestinal mucosa that are described as involved in oral tolerance induction. Frequencies of regulatory-type IEL subsets TCRgd+ and CD8aa are lower in 24-month-old-mice. Production of TGF-b and IL-10 in the small intestine was also reduced during aging. However, mucosal CD4+CD25+Foxp3+ and CD4+LAP+ regulatory T cells increased in mice from 6 months of age. Activated CD4+CD44+ mucosal T cells also augmented. Moreover, the expression of costimulatory molecule CD86 in DCs was augmented and the ability of mucosal dendritic cells to stimulate TGF-b secretion and differentiation of CD4+LAP+ T cells in co-culture studies also declined in 12-month-old-mice. Reduction in these regulatory-type cytokines and T cells may help to explain the decline in susceptibility to oral induction during aging. However, not all mucosal regulatory elements are diminished; CD4+CD25+Foxp3+ as well as CD4+CD25+LAP+ regulatory T cells are augmented and they might play a critical role in maintaining mucosal homeostasis during aging. In addition to its effect in oral tolerance induction, it is possible that aging also affects the time length of this phenomenon. According to some authors, oral tolerance is kept for 21 days to 3 months after feeding. Previous studies by our group showed that oral tolerance can be maintained for a period of one and a half year after oral treatment. These contradictory reports can be explained by different experimental protocols that were used. The main difference in the protocols is related to immunization with antigen + adjuvant that is essential to reveal oral tolerance induction. Thus, we also studied the role of inflammatory events triggered by immunization in the oral tolerance induction and maintenance. For that, BALB/c mice at age of 8-12 weeks were treated orally with 20mg of OVA by gavage and immunized with OVA+Al(OH)3 i.p. Primary immunization varied from 7 to 180 days after oral treatment in mice immunized with OVA+Al(OH)3 and a booster was administered 14 days afterwards. Alternatively, primary immunization with OVA+Al(OH)3 was performed 7 days after oral treatment and booster with OVA varied from 14 to 360 days after primary immunization. We observed that only mice primary immunized up to 90 days after oral treatment were able to sustain oral tolerance to all evaluated parameters. However, mice immunized 7 days after oral treatment were able to keep oral tolerance up to one year after oral treatment. These results suggest that immunization with antigen + adjuvant can act strengthening oral tolerance maintenance. The concomitant injection of antigen and adjuvant and the site of immunization were also important for oral tolerance maintenance. In addition, there was an increase in the frequency of Tregs cells CD4+CD25+Foxp3+ and CD4+CD25+LAP+ in DO11.10 OVA transgenic TCR mice after antigen feeding. Moreover, in C57BL/6 and GFP Foxp3+ knockin mice an increase percentage of these could be seem after oral treatment with antigen followed by immunization with OVA+Al(OH)3. When we searched for the possible mechanism by which Al(OH)3 helps in oral tolerance maintenance neither antigen deposits nor uric acid seem to be necessary. We can conclude that many changes in the gut mucosal associated immune system occurs that can be involved in oral tolerance decline in aged mice. In addition, the interval between oral treatment and primary immunization with OVA+Al(OH)3, the concomitant presence of antigen and adjuvant and the site of injection all affect oral tolerance maintenance.Está bem descrito que o envelhecimento está associado com o declínio da indução de tolerância oral, a qual é iniciada na superfície da mucosa intestinal. Camundongos se tornam menos susceptíveis com 24 semanas de idade e totalmente refratários à indução de tolerância com 70 semanas. Nesses camundongos idosos, somente um regime ótimo de administração oral como a ingestão voluntária, mas não a gavagem, é capaz de torná-los tolerantes ao antígeno ingerido. Assim, avaliamos o efeito do envelhecimento em células T e citocinas da mucosa intestinal comprovadamente envolvidos na indução de tolerância oral. A freqüência de populações de células intraepiteliais (IELs) com fenótipo regulador, como TCRgd+ e CD4+CD8aa mostraram-se reduzidas em animais de 24 meses de idade. A produção de TGF-b e IL-10 também se mostraram diminuídas no intestino delgado de camundongos idosos. No entanto, células T reguladoras CD4+CD25+Foxp3+ e CD4+LAP+ assim como células T ativadas CD4+CD44+ estavam aumentadas na mucosa de camundongos a partir do 6 meses de idade. Além disso, a expressão da molécula co-estimuladora CD86 em células dendríticas (DCs) encontrava-se aumentada em camundongos de 12 meses de idade e a capacidade de DCs de camundongos idosos em estimular a secreção de TGF-b e a diferenciação de células CD4+LAP+, em estudos de co-cultura, também estava diminuída. A redução na produção de citocinas e na freqüência de células T com fenótipo regulador pode estar envolvida no declínio da susceptibilidade à indução de tolerância oral que acompanha o envelhecimento. Entretanto, nem todos os elementos reguladores estavam reduzidos, células com fenótipo regulador CD4+CD25+Foxp3+ e CD4+CD25+LAP+ apresentaram-se aumentadas, e podem ter um papel importante na manutenção da homeostase da mucosa intestinal durante o envelhecimento. Além do seu efeito sobre a indução de tolerância oral, é possível que o envelhecimento também afete o tempo de duração desse fenômeno. De acordo com alguns autores a tolerância oral é mantida por um período curto de 21 dias a 3 meses após a administração oral do antígeno. Trabalhos anteriores de nosso grupo mostraram, no entanto, que a tolerância oral pode ser mantida por até um ano e meio após o tratamento oral. Esses relatos contraditórios podem ser explicados pelos diferentes protocolos experimentais que foram utilizados. A principal diferença nos protocolos está relacionada à imunização com antígeno+adjuvante após o tratamento oral. Em geral, a imunização é utilizada para testar a indução da tolerância oral, mas esse evento inflamatório parece afetar sua manutenção. Neste trabalho, também avaliamos o papel dos eventos inflamatórios desencadeados pela imunização na indução e manutenção da tolerância oral. Para isto, camundongos BALB/c com idade de 8-12 semanas foram tratados por via oral por gavagem com 20mg de OVA e imunizados com OVA+Al(OH)3 i.p. A imunização primária variou de 7 a 180 dias após o tratamento oral e o desafio com OVA i.p. dado 14 dias. Alternativamente, os animais foram imunizados com OVA+Al(OH)3 i.p. 7 dias após o tratamento oral e o desafio com OVA i.p. variou de 14 a 360 dias após a imunização primária. Observamos que somente camundongos que receberam imunização primária até 90 dias após o tratamento oral foram capazes de manter a tolerância oral para todos os parâmetros avaliados. Entretanto, camundongos imunizados 7 dias após o tratamento oral mantiveram a tolerância oral por até um ano após a ingestão do antígeno. Esses resultados sugerem que a imunização com antígeno+adjuvante pode fortalecer a manutenção da tolerância oral. A injeção concomitante de antígeno e adjuvante assim como o sítio de imunização também se mostraram fatores importantes na manutenção da tolerância oral. Além disto, observamos um aumento na freqüência de células T reguladoras CD4+CD25+Foxp3+ e CD4+CD25+LAP+ em camundongos DO11.10 que contam com 85% de linfócitos T expressando TCR transgênico para OVA após o tratamento oral com o antígeno ovalbumina. Um aumento na frequência dessas células também pode ser visto em camundongos C57BL/6 Foxp3-GFP knockin após o tratamento oral com antígeno seguido por imunização com OVA+Al(OH)3. Quando avaliamos os possíveis mecanismos pelos quais o Al(OH)3 reforçou a manutenção da tolerância oral, observamos que nem a formação de depósitos de antígeno nem a liberação de ácido úrico se mostraram necessários. Concluímos que muitas alterações associadas ao envelhecimento ocorrem no tecido linfóide associado à mucosa. Muitas delas podem estar envolvidas na reduzida susceptibilidade à indução de tolerância oral e no declínio da mesma em camundongos idosos. Além disto, o intervalo entre o tratamento oral e a imunização com OVA+Al(OH)3, a presença concomitante do antígeno e do adjuvante e a via de imunização afetaram o tempo de manutenção da tolerância oral. O mecanismo pelo qual o adjuvante Al(OH)3 prolonga o estado de tolerância oral não foi determinado e mais estudos são necessários para se obter uma resposta.
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Desai, Chandni. "Retrospective histopathologic evaluation of fibroblastic versus myofibroblastic entities of the oral mucosa." Thesis, University of Iowa, 2016. https://ir.uiowa.edu/etd/6094.
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The objective of this study is to use immunohistochemistry to determine whether myofibroma is a distinct entity or a variation of other fibroblastic entities. Staining patterns of vimentin, SMA and CD34 are evaluated in irritation fibromas, ulcerated fibromas, giant cell fibromas, inflammatory fibrous hyperplasias, focal keratosis and chronic mucositis, used in this study as a control group, and myofibromas. This study assesses whether myofibromas demonstrate similar staining patterns of vimentin, SMA and CD34 to the aforementioned lesions and if these stains are adequate in differentiating the entities.
Upon IRB approval, 46 cases of irritation fibroma, 45 cases of ulcerated irritation fibroma, 44 cases of giant cell fibroma, 47 cases of focal keratosis and chronic mucositis, and 23 cases of myofibroma were collected. Hematoxylin and eosin-stained slides were reviewed and immunohistochemistry of vimentin, SMA and CD34 was performed. Staining patterns were graded as 0, 1 and 2 (≤25%; ≤25-50%; and ≥50% respectively). Differences were analyzed by the Kruskal-Wallis exact test and Dunn's test for pairwise comparisons.
Vimentin demonstrated grade 2 positivity in all groups with 6.5% and 4.4% of irritation fibromas and myofibromas demonstrating grade 1 positivity, respectively. Smooth muscle actin was strongly positive in myofibroma (78.3%, grade 2, p < 0.05) but also demonstrated positivity in focal keratosis and chronic mucositis (2.1% grade 1), giant cell fibromas (4.6% grade 1, 4.6% grade 2), inflammatory fibrous hyperplasias (13.6% grade 1, 13.6% grade 2) and ulcerated irritation fibromas (6.7% grade 2, 8.9% grade 1) though this was not statistically significant. CD34 demonstrated statistically significant positivity in irritation fibromas (87% grade 2, 2.2% grade 1), ulcerated irritation fibromas (73.3% grade 2, 6.7% grade 1), and inflammatory fibrous hyperplasias (63.6% grade 2, 6.8% grade 1). CD34 also demonstrated positivity in giant cell fibromas (54.6% grade 2, 2.3% grade 1) and myofibromas (21.7% grade 2, 4.4% grade 1) though this was not statistically significant. The Dunn’s test for pairwise comparisons revealed statistically significant differences for CD34 staining between myofibroma and fibroma; myofibroma and focal keratosis and chronic mucositis; myofibroma and inflammatory fibrous hyperplasia; and between myofibroma and ulcerated fibroma.
The results indicate that smooth muscle actin immunohistochemistry is helpful but not definitive in delineating myofibroma as a separate entity. In addition, as other fibrous proliferations in our study also expressed smooth muscle actin, it is possible that smooth muscle actin expression correlates with the duration and chronic tissue assault associated with a lesion, thus supporting the theory that oral myofibromas are part of the spectrum of fibrous proliferations. The results also indicate that although CD34 is deemed a specific marker for solitary fibrous tumor, this marker was not reliable in distinguishing any of the entities from one another and may in fact not be specific for solitary fibrous tumor. Further studies are needed to support this claim.
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Tupper, Satt Andrea Cecilia. "Daño oxidativo en individuos fumadores y no fumadores con mucosa oral clínicamente sana utilizando citología oral en base líquida." Tesis, Universidad de Chile, 2006. http://repositorio.uchile.cl/handle/2250/144008.
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Trabajo de Investigación Requisito para optar al Título de Cirujano DentistaLos individuos fumadores presentan cambios invisibles en la mucosa oral clínicamente sana, los cuales han sido detectados en estudios citológicos. No ha sido adecuadamente establecido si el tabaco es capaz de producir daño oxidativo en el ADN de las células de la mucosa oral expuesta al tabaco, mecanismo que podría estar involucrado en la carcinogénesis oral. El objetivo de este trabajo fue detectar daño oxidativo en células de la mucosa oral del borde de lengua de individuos fumadores y no fumadores y asociarlo con las variables edad e intensidad del hábito de fumar. Además, cuantificar los tipos de células queratinizadas. Se obtuvieron 30 muestras citológicas de individuos fumadores de más de 10 cigarrillos al día por más de 5 años y una muestra pareada por edad y sexo de individuos no fumadores, ambos grupos con mucosa oral clínicamente sana. Las muestras fueron obtenidas con cepillos para frotis Cervex brush Prep R R , procesadas con el sistema de citología en base líquida Thin Pap system de Cytyc Corporations UK y teñidas con el anticuerpo monoclonal para la detección de 8-OHdG (8 –hydroxyguanine) principal marcador de estrés oxidativo en el ADN y, además con PAP. La intensidad de tinción nuclear fue cuantificada utilizando el programa de procesamiento de imágenes Image J (NIH). Encontramos que los individuos fumadores presentaron un valor promedio de tinción nuclear mayor que los no fumadores, 183.21; DS. 15.87 y 160.95; DS. 20.84 (p=0,000019) respectivamente. No encontramos relación entre el promedio de tinción nuclear y la edad e intensidad del hábito de fumar. Además se observaron porcentajes similares de los tipos de células queratinizadas en fumadores y no fumadores. Concluimos que las células de la mucosa oral del borde de lengua de los individuos que fuman presentaban mayor daño oxidativo en el ADN, evidenciables con técnicas citológicas no invasivas. Esta metodología podría utilizarse para el monitoreo de pacientes con hábitos asociados a mayor riesgo de desarrollo de cáncer oral.
Financiado por Proyecto DID de Iniciación I-12 03/10-4 Vicerrectoría de Asuntos Académicos. Universidad de Chile.