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Dissertations / Theses on the topic 'Orf63'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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1

Hoppe, Anne. "Investigation of E4 Orf3-mediated ND10 rearrangement." Thesis, University of Warwick, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479289.

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2

Pliquet, Daniel [Verfasser]. "Role of BET-proteins in the function of rhadinoviral Orf73-proteins / Daniel Pliquet." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2011. http://d-nb.info/1013289927/34.

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3

Gurmu, Daniel. "Structural Studies of Microbial Proteins - From Escherichia coli and Herpesviruses." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-35674.

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Structure biology concerns the study of the molecular structures of biological macromolecules, such as proteins, and how these relate to the function. Protein structures are also of importance in structure-based drug design. In this thesis, the work has been carried out in two different projects. The first project concerns structural studies of proteins from the bacterium Escherichia coli and the second of proteins from five different herpesviruses. The E. coli project resulted in the structural characterization of three proteins: CaiB, RibD, and YhaK. CaiB is a type-III CoA transferase invol

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4

Koyuncu, Emre. "Expression, Purification, And Functional Analysis Of Adenovirus Type 5 E4 Orf3 Protein." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/12605415/index.pdf.

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In this study, structural and functional aspects of adenovirus type 5 (Ad5) E4orf3 protein were analyzed by biophysical and biochemical methods. Ad5 is one of the mostly used gene therapy vectors to date. However, some of its proteins possess oncogenic potential and their presence comprises safety risks. E4orf3 is one of the oncoproteins of Ad5. It also takes important roles in viral infection, and is beneficial for therapy vectors. Therefore, understanding the functions of E4orf3 is very important for developing efficient and safe adenovirus vectors. Most of the present knowledge about the f

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5

Horridge, Jackie J. "RNA-protein interactions of the adenovirus proteins E1B 55K and E4 Orf6." Thesis, University of Warwick, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322435.

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6

Liedeman, Kerwin. "Transient transgene expression of human Coronavirus nl63 orf3 protein in a baculovirus system." University of the Western Cape, 2020. http://hdl.handle.net/11394/8042.

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>Magister Scientiae - MSc<br>Insect-derived baculoviruses have been used extensively as a safe and versatile research model for transgenic protein expression. Preclinical studies have revealed the promising potential of Baculoviruses as a delivery vector for a variety of therapeutic applications, including vaccination, tissue engineering and cancer treatments. Coronaviruses are enveloped viruses containing linear, non-segmented ribonucleic acid. Human coronavirus NL63 was first discovered in the Netherlands in January 2004, where a 7-month-old girl presented with an acute respiratory tract inf

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7

Fisher, Randall Graeme. "Cloning of Human Coronavirus NL-63 ORF3, M and E genes for antibody production." Thesis, University of the Western Cape, 2010. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_7572_1361368796.

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<p>Human Coronavirus NL-63 is a respiratory virus with a high incidence rate, causing mild respiratory infections in children under the age of 18. The outbreak of Sever Acute Respiratory&nbsp<br>Syndrome (SARS) in 2003 sparked increased interest into the field of coronavirology and respiratory diseases subsequently led to the discovery of this novel Human Coronavirus (HCoV) by a group of scientists in Holland. The membrane protein (M) of NL-63 has been shown to interact with the nucleocapsid, spike and envelope proteins of the virus when expressed ex vivo. In contrast, the envelope protein (E)

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8

Kannan, Harilakshmi. "Functional characterization of the interaction of hepatitis E virus ORF3 product with the cytoskeleton." College Park, Md.: University of Maryland, 2008. http://hdl.handle.net/1903/8969.

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Thesis (M.S.) -- University of Maryland, College Park, 2008.<br>Thesis research directed by: Virginia-Maryland Regional College of Veterinary Medicine. Maryland Campus. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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9

Cham, Kevin Sae Wing. "Further analysis of the ORF73 and ORF74 genes of the murid herpesvirus 4 in the natural host." Thesis, University of Liverpool, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.526855.

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10

Melling, Michael [Verfasser], and Thomas [Akademischer Betreuer] Dobner. "The influence of SUMOylation on the adenoviral early region 4 protein Orf6/7 / Michael Melling ; Betreuer: Thomas Dobner." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2018. http://d-nb.info/1163013692/34.

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11

津下, 誠治. "カリフラワーモザイクウイルスの増殖機構に関する研究 : ORF1とORF3の遺伝子産物の役割". 京都大学, 2000. http://hdl.handle.net/2433/151627.

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12

Müller, Marcel Alexander [Verfasser]. "Studies on human pathogenic coronaviruses survey for coronaviruses in African bat species and characterization of the novel human coronavirus NL63 (HCoV-NL63) open reading frame 3 (ORF3) / Marcel Alexander Müller." Berlin : Freie Universität Berlin, 2008. http://d-nb.info/1022641670/34.

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13

Wang, Shie Shan, and 王世憲. "Transcriptional regulation of the ORF61, ORF60 and ORF46 genes of Kaposi's sarcoma-associated herpesvirus." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/95933108931858843276.

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博士<br>長庚大學<br>臨床醫學研究所<br>100<br>Kaposi’s sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus closely associated with all forms of Kaposi’s sarcoma (KS) as well as lymophoproliferative disorders, including primary effusion lymphoma and multicentric Castleman’s disease. An important step for viral propagation and pathogenesis is the switch from latency to the lytic cycle. The viral gene expression in the lytic cycle program is controlled tightly and expressed in an orderly fashion. In the study, we characterized the transcriptional regulation of three viral genes ORF61, ORF60 and ORF46 d

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14

Wang, Jen-Yi, and 王貞懿. "Protein purification and bacterial inhibition analysis of ORF693 of phage fkm18p." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/y389sv.

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碩士<br>義守大學<br>化學工程學系暨生物技術與化學工程研究所<br>102<br>Acinetobacter baumannii is an acidiphilic, obligate, aerobic and gram-negative bacterium. It is an opportunistic pathogen and people will fall to ill when they were under weak immunity. We have isolated several lytic bacteriophages from the environment to lysis A. baumannii and the km18p has the highest lytic efficiency. Phage km18p provided 100% protection and survival rate of mice that were inoculated with A. baumannii KM18. So this study will focus on clonong the lysin gene and detecting the bacterial inhibition efficiency of the expressed prote

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15

Cerqueira, Sofia Isabel Arriaga Mimoso 1988. "Identification of NF-kB and MYC binding motifs in Herpesvirus ORF73." Master's thesis, 2011. http://hdl.handle.net/10451/6509.

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Tese de mestrado. Biologia (Microbiologia Aplicada). Universidade de Lisboa, Faculdade de Ciências, 2011<br>O herpesvírus murídeo tipo 4, MuHV-4, é um gama-herpesvírus e, enquanto tal, estabelece infecções latentes em tecidos linfóides. Durante a infecção latente, o genoma de MuHV-4 persiste sob a forma de um epissoma no núcleo das células latentemente infectadas, e várias proteínas são expressas, entre as quais a proteína ORF73. Além do seu papel putativo na manutenção do epissoma viral, ORF73 modula os factores de transcrição celulares NF-kB e MYC, através do recrutamento das proteínas celul

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16

Ya-Hui, Wu, and 吳雅惠. "Expression of ORF3 Gene of Porcine Reproductive and Respiratory Syndrome Virus." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/90633540532245310694.

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碩士<br>國立臺灣大學<br>獸醫學研究所<br>90<br>To validate the character of the GP3 of porcine reproductive and respiratory syndrome virus (PRRSV), full-length of open reading frame 3 (ORF3) gene of Taiwanese strain MD-001 was fused with eukaryotic green fluorescent protein (GFP) expression vector. The results showed that GFP/ORF3 fusion protein was localized mainly in the cytoplasm and then accumulated progressively at one-side of cells, whereas the GFP distributed throughout the whole expressing cells. The amount of GFP/ORF3-expressing cells was rare while most of them were rounded-up. Western blot analysi

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17

LEE, Tzu-Ying, and 李姿穎. "Molecular Mechanism of the type I interferon antagonist function by SARS Coronavirus ORF6 protein." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/75653793395898183769.

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碩士<br>中國醫藥大學<br>醫學檢驗生物技術學系<br>96<br>Abstract Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease. The causative agent of SARS has been identified to be a new type of coronavirus, namely SARS coronavirus (SARS-CoV).SARS-CoV proteins including the ORF6 protein have been reported to inhibit interferon signaling response. The goal of this study is to identify human SARS-CoV ORF6-interacting proteins using the phage displayed human lung cDNA libraries and to investigate their interaction on the type I interferon antagonist function. At first, the recombinant ORF6 protein

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18

Lin, Wei Li, and 林維莉. "Expression and functional analysis of the porcine circovirus type 2 Rep and ORF3 proteins." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/04865829915580224077.

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博士<br>國立中興大學<br>微生物暨公共衛生學研究所<br>100<br>Porcine circovirus type 2 (PCV2) is a member belongs to the genus Circovirus of the Circoviridae family, and closely associated with a disease syndrome in pigs described as “postweaning multisystemic wasting syndrome” (PMWS) now known as porcine circovirus diseases (PCVD). The genome of PCV2 is a single-stranded circular DNA, and contains three major open reading frames (ORFs). They are ORF1, the rep gene, which encodes the Rep proteins responsible for virus replication; and ORF2, the cap gene, which encodes the immunogenic capsid (Cap) protein; and ORF3

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19

Lan, Shun-Han, and 藍順瀚. "Construction of infectious clone and functional study of the ORF3 gene from duck circovirus." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/59991094775123078913.

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碩士<br>國立中興大學<br>微生物暨公共衛生學研究所<br>100<br>Infections with duck circovirus (DuCV) are associated with growth retardation and developmental problems in farmed ducks. DuCV invades lymphoid tissues and lead to immunosuppression and an increased probability of secondary infections. This disease has been the cause of considerable economic losses to the duck industry worldwide. Because no in vitro culture system is yet available for propagation of DuCV, the pathogenesis of this virus remains unclear. The availability of infectious clones offers an opportunity for analysis and modification of viral genom

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20

Shyu, Duan-Liang, and 徐敦良. "Antigenic Analysis of ORF1, OR2 and ORF3 of Porcine Circovirus Type 2 Expressed Viral Peptides." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/76665853457948582976.

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碩士<br>國立臺灣大學<br>獸醫學研究所<br>92<br>Porcine circovirus type 2 (PCV2)cause PMWS in weaning piglets, and was found highly infectious in pug farms. Polymerase chain reactions (PCR) was used to amplify ORF1, ORF2 and ORf3 of PCV2 from infected lymph node emulsion by using specific primers. E. coli expression vector pRSET A and DNA vaccine vactor pcDNA3 were used as the plasmid to clone the 3 ORFs of PCV2, respectively. Recombinant proteins of ORF1, ORF2 and ORF3 of PCV2 were expressed in E. coli were analyzed by SDS-PAGE and western blotting. The DNA vector vaccine were used to immulized rabbits by in

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21

Dan, Tran Quang, and 陳光瑩. "Studies on the Expression of Recombinant ORF3 Fusion Proteins of Porcine Circovirus Type 2 in Transgenic Tobacco." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/xwceag.

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碩士<br>中國文化大學<br>生物科技研究所<br>102<br>Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome in pigs and has impact on swine-producing industry worldwide. The open reading frame 3 (ORF3) of PCV2 which encodes an 11.9 kDa ORF3 protein has been found to be involved in the pathogenesis of PCV2 infection by its apoptotic activity. The ORF3–based antigens thus represent a traditional approach to develop efficacious oral vaccines for immunization against PCV2. In this study, various ORF3-containing expression vectors, namely pGKF/U-ORF3, pGKF/U-L-ORF3, pGK

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22

Ottinger, Matthias [Verfasser]. "Functional characterisation of γ2-herpesviral [gamma-2-herpesviral] orf73 proteins and their interaction with the cellular proteins BRD2 and BRD4 / by Matthias Ottinger". 2005. http://d-nb.info/976181819/34.

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