To see the other types of publications on this topic, follow the link: Organell.
Dissertations / Theses on the topic 'Organell'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Organell.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Schwarz, Elisabeth. "Die Rolle des mitochondrialen Hsp70-Systems bei verschiedenen Prozessen der Organell-Biogenese." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961717335.
Full text
2
Cerjan, Dijana. "INTRACELLULAR DISTRIBUTION PATTERNS OF ORGANELL SPECIFIC PROTEINS USING IMMUNOHISTOCHEMICAL STAINING OF TISSUE MICRO ARRAYS." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6154.
Full textAbstract:
The knowledge of the human genome sequence, as revealed in the HUGO project, has created exciting new possibilities for biomedical research. The Swedish Human Proteome Resource (HPR) program aims to make use of this information to gain further insight into the human proteome. Recombinant proteins are generated from coding sequences identified from the human genome sequence and used to produce specific antibodies to target proteins. Antibodies are subsequently utilized for functional analysis of the corresponding proteins using tissue micro arrays. The aim of my project was to investigate the possibility of distinguishing characteristic distribution patterns of intracellular proteins in the resolution capacity offered by light microscopy. A map of representative distribution patterns was created using immunohistological staining with commercially available antibodies toward well-characterised proteins in the cell. Such a map could then aid in interpreting the results of immunohistological staining of intracellular proteins using antibodies produced within the Human Proteome Resource program. Proteins manifested in nucleus, nuclear membrane and plasma membrane were clearly visible at the expected location. Proteins manifested in different organelles in the cytoplasm however, showed all a similar staining pattern, making determination of exact protein location uncertain. A possible explanation is the resolution of the light microscope not being sufficient to visualize certain proteins specific to organelles in the cytoplasm. Results may also have been influenced by the choice of secondary antibody, where the strenghtened signal generated by an enzyme labelled polymer may have a negative effect on depiction of details in the image generated.
3
Freitag, Johannes [Verfasser], and Michael [Akademischer Betreuer] Bölker. "Neue Enzyme für ein altes Organell : kryptische peroxisomale Lokalisationssignale in Pilzen / Johannes Freitag. Betreuer: Michael Bölker." Marburg : Philipps-Universität Marburg, 2014. http://d-nb.info/1050816994/34.
Full text
4
Mietner, Silke. "Charakterisierung von Organellen und Signalwegen des Thrombozyten." kostenfrei, 2008. http://www.opus-bayern.de/uni-wuerzburg/volltexte/2008/3121/.
Full text
5
Eriksson, Therese. "Organelle movement in melanophores: Effects of Panax ginseng, ginsenosides and quercetin." Licentiate thesis, Linköpings universitet, Farmakologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-19973.
Full textAbstract:
Panax ginseng is a traditional herb that has been used for over 2000 years to promote health and longevity. Active components of ginseng include ginsenosides, polysaccharides, flavonoids, polyacetylenes, peptides, vitamins, phenols and enzymes, of which the ginsenosides are considered to be the major bioactive constituents. Although widely used, the exact mechanisms of ginseng and its compounds remain unclear. In this thesis we use melanophores from Xenopus laevis to investigate the effects of Panax ginseng extract G115 and its constituents on organelle transport and signalling. Due to coordinated bidirectional movement of their pigmented granules (melanosomes), in response to defined chemical signals, melanophores are capable of fast colour changes and provide a great model for the study of intracellular transport. The movement is regulated by alterations in cyclic adenosine 3’:5’-monophosphate (cAMP) concentration, where a high or low level induce anterograde (dispersion) or retrograde (aggregation) transport respectively, resulting in a dark or light cell. Here we demonstrate that Panax ginseng and its constituents ginsenoside Rc and Rd and flavonoid quercetin induce a concentration-dependent anterograde transport of melanosomes. The effect of ginseng is shown to be independent of cAMP changes and protein kinase A activation. Upon incubation of melanophores with a combination of Rc or Rd and quercetin, a synergistic increase in anterograde movement was seen, indicating cooperation between the ginsenoside and flavonoid parts of ginseng. Protein kinase C (PKC) inhibitor Myristoylated EGF-R Fragment 651-658 decreased the anterograde movement stimulated by ginseng and ginsenoside Rc and Rd. Moreover, ginseng, but not ginsenosides or quercetin, stimulated an activation of 44/42-mitogen activated protein kinase (MAPK), previously shown to be involved in both aggregation and dispersion of melanosomes. PKC-inhibition did not affect the MAPK-activation, suggesting a role for PKC in the ginseng- and ginsenoside-induced dispersion but not as an upstream activator of MAPK.Panax ginseng är ett av de vanligaste naturläkemedlen i världen och används traditionellt för att öka kroppens uthållighet, motståndskraft och styrka. Ginseng är ett komplext ämne bestående av ett antal olika substanser, inklusive ginsenosider, flavonoider, vitaminer och enzymer, av vilka de steroidlika ginsenosiderna anses vara de mest aktiva beståndsdelarna. Flavonoider (som finns i till exempel frukt och grönsaker) och ginseng har genom forskning visat sig motverka bland annat hjärt-och kärlsjukdomar, diabetes, cancer och demens. Trots den omfattande användningen är dock mekanismen för hur ginseng verkar fortfarande oklar. I den här studien har vi använt pigmentinnehållande celler, melanoforer, från afrikansk klogroda för att undersöka effekterna av Panax ginseng på pigment-transport och dess maskineri. Melanoforer har förmågan att snabbt ändra färg genom samordnad förflyttning av pigmentkorn fram och tillbaka i cellen, och utgör en utmärkt modell för studier av intracellulär transport. Förflyttningen regleras av förändringar i halten av cykliskt adenosin-monofosfat (cAMP) i cellen, där en hög eller låg koncentration medför spridning av pigment över hela cellen (dispergering) eller en ansamling i mitten (aggregering), vilket resulterar i mörka respektive ljusa celler. Här visar vi att Panax ginseng, ginsenosiderna Rc och Rd samt flavonoiden quercetin stimulerar en dispergering av pigmentkornen. När melanoforerna inkuberades med en kombination av ginsenosid Rc eller Rd och quercetin, kunde en synergistisk ökning av dispergeringen ses, vilket tyder på en samverkan mellan ginsenosid- och flavonoid-delarna av ginseng. Ett protein som tidigare visats vara viktigt för pigmenttransporten är mitogen-aktiverat protein kinas (MAPK), och här visar vi att också melanoforer stimulerade med ginseng, men dock inte med ginsenosider eller quercetin, innehåller aktiverat MAPK. Genom att blockera enzymet protein kinas C (PKC) (känd aktivator av dispergering), minskade den ginseng- och ginsenosid-inducerade dispergeringen, medan aktiveringen av MAPK inte påverkades alls. Detta pekar på en roll för PKC i pigment-transporten men inte som en aktivator av MAPK.
6
Berglund, Jenny. "Structure-function studies of organelle assembly and receptor recognition in organelles assembled via the chaperone/usher pathway /." Uppsala : Dept. of Molecular Biology, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/a441.pdf.
Full text
7
Morley, Stewart Anthony. "Interactions Between the Organellar Pol1A, Pol1B, and Twinkle DNA Replication Proteins and Their Role in Plant Organelle DNA Replication." BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/8128.
Full textAbstract:
Plants maintain organelle genomes that are descended from ancient microbes. Ages ago, these ancient microbes were engulfed by larger cells, beginning a process of co-evolution we now call the endo-symbiotic theory. Over time, DNA from the engulfed microbe was transferred to the genome of the larger engulfing cell, eventually losing the ability to be free-living, and establishing a permanent residency in the larger cell. Similarly, the larger cell came to rely so much on the microbe it had engulfed, that it too lost its ability to survive without it. Thus, mitochondria and plastids were born. Nearly all multicellular eukaryotes possess mitochondria; however, different evolutionary pressures have created drastically different genomes in plants versus animals. For one, animals have very compact, efficient mitochondrial genomes, with about 97% of the DNA coding for genes. These genomes are very consistent in size across different animal species. Plants, on the other hand, have mitochondrial genomes 10 to more than 100 times as large as animal mitochondrial genomes. Plants also use a variety of mechanisms to replicate and maintain their DNA. Central to these mechanisms are nuclear-encoded, organelle targeted replication proteins. To date, there are two DNA polymerases that have been identified in plant mitochondria and chloroplasts, Pol1A and Pol1B. There is also a DNA helicase-primase that localizes to mitochondria and chloroplasts called Twinkle, which has similarities to the gp4 protein from T7 phage. In this dissertation, we discuss the roles of the polymerases and the effects of mutating the Pol1A and Pol1B genes respectively. We show that organelle genome copy number decreases slightly and over time but with little effect on plant development. We also detail the interactions between Twinkle and Pol1A or Pol1B. Plants possess the same organellar proteins found in animal mitochondria, which are homologs to T7 phage DNA replication proteins. We show that similar to animals and some phage, plants utilize the same proteins in similar interactions to form the basis of a DNA replisome. However, we also show that plants mutated for Twinkle protein show no discernable growth defects, suggesting there are alternative replication mechanisms available to plant mitochondria that are not accessible in animals.
8
Malchus, Nina Isabelle [Verfasser], and Michael [Akademischer Betreuer] Hausmann. "On the spatial organization of cell organelles and diffusion of proteins in organelle membranes / Nina Isabelle Malchus ; Betreuer: Michael Hausmann." Heidelberg : Universitätsbibliothek Heidelberg, 2011. http://d-nb.info/1179230477/34.
Full text
9
González, González Luis. "Functional and structural analyses of the terminal organelle of Mycoplasma genitalium." Doctoral thesis, Universitat Autònoma de Barcelona, 2015. http://hdl.handle.net/10803/326466.
Full textAbstract:
Mycoplamsa genitalium es un patógeno humano causante de uretritis no gonocócica y no causada por clamidias en hombres y de enfermedad pélvica inflamatoria y cervicitis. Los micoplasmas, además de ser muy interesantes para el estudio como células mínimas (dado el pequeño tamaño de su genoma), también presentan características únicas sólo presentes en este género. En particular, se ha detectado la presencia de mecanismos de adhesión y de motilidad, que además de estar implicados en el mecanismo de infección, sólo se encuentran en este género. En especial, el mecanismo de motilidad de M. genitalium consta de una estructura polar que contiene un característico citoesqueleto. Se conoce que este citoesqueleto está formado por varias proteínas implicadas en el proceso de adhesión y motilidad. En los tres primeros capítulos de esta tesis se ha determinado el papel de tres de estas proteínas: MG219, MG318 (también llamada P32) y MG386. El estudio se ha realizado gracias a la obtención de mutantes defectivos de estas proteínas. La proteína MG219 se ha hallado que es necesaria para el correcto funcionamiento de la maquinaria de motilidad de este organismo. Mediante fusión a proteínas fluorescentes se ha determinado la localización subcelular de esta proteína, que resulta localizar en la parte más cercana de la organela terminal respecto al cuerpo celular. Las células en ausencia de MG219 se mueven a una velocidad inferior a la mitad (y la mitad de frecuentemente) que las células de la cepa salvaje. Esta reducción de la velocidad es concomitante con la aparición de un mayor número de células en división y con múltiples organelas terminales. De una manera similar, las células de la cepa que carece la proteína P32 se mueven a velocidades inferiores (y con la mitad de frecuencia) que las células de la cepa salvaje. Además, se ha determinado que el N-terminal de P32 juega un papel importante en la estabilidad de las proteínas P110 y P140, las más importantes adhesinas de M. genitalium. También se ha establecido que la proteína P32 es crítica para la morfología de la parte más distal de la organela terminal. El último mutante generado en este trabajo, el defectivo para la proteína MG386, presenta importantes variaciones tanto a nivel de morfología celular como de motilidad. Las células de esta cepa presentan motilidad la mitad de frecuentemente que la cepa salvaje pero se mueven a una velocidad 1.7 veces superior. Sorprendentemente, esta cepa presenta un gran número de organelas terminales desancladas del cuerpo celular, sugiriendo un importantísimo papel de la proteína MG386 en el anclaje de la organela terminal al cuerpo celular.
Se ha observado que la membrana alrededor del citoesqueleto se encuentra completamente recubierta por el complejo de adhesión o ‘nap’. Mediante estudios de microscopia electrónica se ha determinado la estructura a 3.5 nm por ctomografia crioelectrónica y a 1.9 nm por tinción negativa. Además, la tomografía crioelectrónica también ha desvelado la estructura a baja resolución del botón terminal del citoesqueleto, revelando que las placas que forman la mayor parte del citoesquelto son, en realidad, anillos de unos 20 nm de diámetro. Además, se han analizado por tomografía crioelectrónica 14 mutantes que carecen de diferentes proteínas (o dominios de éstas) relacionadas con motilidad y/o adhesión. El conjunto de todos estos datos aporta una visión global al conocimiento previo (y al generado en este trabajo) sobre el papel de las proteínas implicadas motilidad y formación del citoesqueleto de M. geniatlium.Mycoplamsa genitalium is a human pathogen and the causative agent of non-gonococcal non-chlamydial urethritis in men and pelvic inflammatory disease and cervicitis. Mycoplasmas, besides being interesting as minimal cells (given the small size of its genome), also have unique features only present in its genus. In particular, the presence of mechanisms of adhesion and motility has been detected, and in addition of being involved in addition of being involved in the infection mechanism, are only found in this genus. In particular, the mechanism of motility of M. genitalium involves a polar structure containing characteristic cytoskeleton. It is known that the cytoskeleton is composed of several proteins involved in the adhesion and motility processes. In the first three chapters of this thesis dissertation the role of three of this proteins—MG219, MG318 (also called P32) and MG386—has been stablished. The study was conducted by obtaining null mutants strains of these proteins. The MG219 protein has been found to be necessary for the proper functionality of the motility machinery. By fusion to fluorescent proteins it has been determined the subcellular localization of this MG219, which located at the nearest part of the terminal organelle relative to the cell body. Cells in the absence of MG219 move slower than half speedy (and half frequently) the cells of the wild type strain. This speed reduction is concomitant with the appearance of a greater number of dividing cells and cell with multiple terminal organelles. In a similar manner, cells of the strain lacking P32 move at lower speeds (and with half also half frequently) than cells of the wild type strain. Furthermore, it has been determined that the N-terminal P32 plays an important role in protein stability P110 and P140, the major adhesins of M. genitalium. It has also been established that the P32 protein is critical to the morphology of the most distal part of the terminal organelle relative to the cell body. The last mutant generated in this work, the null mutant for MG386, presents significant alterations in both cell morphology and motility. The cells of this strain show motility half frequently than the wild type strain but move to a velocities as greater as1.7 times than the reference strain. Surprisingly, this strain has a high number of terminal organelles detached from the cell body, suggesting an important role of protein MG386 anchoring the organelle to the cell body. It has been observed that the membrane around the cytoskeleton is completely covered by the adhesion complex or "nap". By electron microscopy studies it has determined the structure by cryo-electron tomography at 3.5 nm and at 1.9 nm single particle by negative staining TEM of the purified P110 and P140 complex. Additionally, cryo-electron tomography also allowed to determine at low resolution the structure of the terminal button of the cytoskeleton, revealing that the plates forming most of the cytoskeleton are actually rings about 20 nm in diameter. In addition, 14 mutants lacking different proteins (or domains thereof) related to motility and / or adhesion have been analysed by cryo-electron tomography. All these data taken together provides an overview of the prior knowledge—in addition to the data generated in this work—of the role of the proteins involved motility and cytoskeleton formation in M. geniatlium.
10
Mahon, Piers Seaburne Macmahon. "Localisation of organelle proteins." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621831.
Full text
11
Silva, Saura Rodrigues da. "Genômica organelar e evolução de Genlisea e Utricularia (lentibulariaceae)." Botucatu, 2018. http://hdl.handle.net/11449/153889.
Full textAbstract:
Orientador: Vitor Fernandes Oliveira de MirandaResumo: Utricularia e Genlisea são gêneros irmãos de plantas carnívoras da família Lentibulariaceae. Possuem aproximadamente 260 espécies representadas em diversas formas de vida. Para o Brasil foram catalogadas 82 espécies, das quais 27 são consideradas endêmicas. Além de dispor das armadilhas carnívoras mais complexas entre plantas, algumas de suas espécies apresentam os menores genomas e as maiores taxas de mutações entre as angiospermas relatadas até o momento. A respeito de seus genomas organelares, os estudos são pífios. Neste contexto, há a necessidade de se investigar como são os genomas organelares, suas estruturas, seus genes e como se deu a evolução das organelas nos gêneros. Portanto este estudo teve como objetivo, a partir de sequenciamento de nova geração e montagem de genomas, estudar e comparar os genomas organelares de Utricularia e Genlisea. Neste âmbito, foram montados e sequenciados os cloroplastos das espécies Utricularia foliosa, U. reniformis, G. aurea, G. filiformis, G. pygmaea, G. repens e G. tuberosa, e o genoma mitocondrial de U. reniformis. Os resultados obtidos revelaram que possivelmente há relação entre forma de vida e presença de genes ndhs nos gêneros, em razão de que para as espécies terrestres há deleção e “pseudogenização” de genes ndhs, já as espécies aquáticas detêm todo repertório de ndhs intacto. A partir das evidências encontradas, foi possível constatar transferência horizontal de genes, inclusive de genes ndhs, em mitocôndrias.
Abstract: Utricularia and Genlisea are sister genera in the carnivorous family Lentibulariaceae. There are aprproximately 260 species representing diverse life forms. For Brasil there are 82 species, 27 considered endemic. At the moment, besides having the most complex carnivorous traps between all plants, some of its species have miniature genomes and the highest mutational rates among angiosperms. There are few studies regarding its organellar genome. In this context, it is necessary to investigate how are these organellar genomes, its structure, genes, and how evolutionary forces govern these organelles in the different genera. Therefore, the aim of this study is to study and compare the organellar genomes of Utricularia and Genlisea, using next generation sequencing and genome assembly. In this context, chloroplasts of the species Utricularia foliosa, U. reniformis, Genlisea aurea, G. filiformis, G. pygmaea, G. repens and G. tuberosa, and the mitochondrial genome of U. reniformis were assembled and sequenced. The results show that possibly there is a connection between life form and the presence of ndhs genes in the genera, since for the terrestrial species there are ndhs genes that are deleted and pseudogenization, in contrary to the aquatic species which have all intact ndhs repertoir. Concerning the evidences, it was possible to verify horizontal transfer of ndhs and other genes as there are chloroplasts genes in the mitochondria.
Doutor
12
Dunkley, Thomas Peter John. "Mapping the Arabidopsis organelle proteome." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598685.
Full textAbstract:
The work presented in this thesis involves the development of a proteomic method for protein localization that is not dependent on the preparation of pure organelles. This method has subsequently been named localization of organelle proteins by isotope tagging (LOPIT). Organelles are first partially separated using centrifugation through density gradients. Distributions of proteins within such gradients are then assessed by measuring the relative abundance of proteins between fractions along the length of the gradients. This is achieved using isotope-coded tags for protein quantitation by mass spectrometry. The subcellular localizations of proteins can then be determined by comparing their distributions to those of previously localized proteins, since proteins that belong to the same organelle will co-fractionate in the density gradients. Application of the LOPIT technique to the study of the Arabidopsis endomembrane system has resulted in the simultaneous assignment of 527 proteins to the endoplasmic reticulum, Golgi apparatus, vacuole, plasma membrane or to the mitochondria and plastids. These results demonstrate that proteomic analysis of the major endomembrane components can be performed in parallel, enabling protein steady state distribution between these organelles to be determined. Consequently, for the first time by proteomics, genuine residents of the endoplasmic reticulum, Golgi apparatus, vacuole and plasma membrane have been distinguished from contaminants and proteins that are in transit through the secretory pathway.
13
Broto, Hernández Alícia. "Anàlisi funcional de dominis de proteïnes implicades en la motilitat de Mycoplasma genitalium." Doctoral thesis, Universitat Autònoma de Barcelona, 2014. http://hdl.handle.net/10803/285117.
Full textAbstract:
Mycoplasma genitalium és un patogen emergent de transmissió sexual. Aquest microorganisme sense paret cel·lular conté un dels genoma més petit que es coneix entre els organismes autoreplicatius i això el converteix en un model atractiu de cèl·lula mínima. Tot i l’aparent simplicitat, M. genitalium presenta un complex citoesquelet intern que polaritza la cèl·lula i li atorga la característica forma de pera, deguda a la formació d’un apèndix a un extrem de la mateixa. Aquest apèndix es coneix com organela terminal (OT) i està implicat en processos clau de la seva vida parasitària. L’OT té un paper central en la motilitat per lliscament del micoplasma. Aquest mecanisme únic i encara poc caracteritzat està relacionat amb molts aspectes de la seva biologia, amb especial rellevància en la patogènesi. L'objectiu general d'aquest treball és aprofundir en la comprensió d'aquest mecanisme mitjançant l'estudi de la contribució específica de diferents dominis de proteïnes prèviament relacionats amb l'OT o la motilitat.
L’OT s'organitza al voltant del citoesquelet intern. La proteïna MG218 és un dels elements principals del citoesquelet i té un paper central en la formació de l’OT. Recentment, s’ha identificat una nova proteïna que comparteix la seqüència C-terminal de MG218. Aquesta proteïna (MG218-s) és producte d’una traducció interna del gen MG218 però el seu inici de traducció exacte encara no està definit. Per entendre millor la funció d'aquesta proteïna, en el primer capítol, s’ha dissenyat una nova estratègia per introduir mutacions puntuals en el genoma de M. genitalium. Aquesta estratègia ha permès la identificació de la Met inicial de la proteïna MG218-s i s’ha obtingut i caracteritzat un mutant defectiu en MG218-s, però que segueix expressant la proteïna MG218.
El segon i tercer capítol se centren en l'estudi de la proteïna MG200, que és un element important de la maquinària de la motilitat. Les proteïnes MG200 i MG386 són els primers elements que s’han relacionat exclusivament amb la motilitat de M. genitalium. Aquestes proteïnes comparteixen diferents característiques, essent destacable la presència d'un domini ric en glicina i residus aromàtics (caixa EAGR), que es troba exclusivament en micoplasmes, aparentment sempre en proteïnes relacionades amb la motilitat i/o l'arquitectura de l’OT, com la MG312. L'anàlisi de l'estructura cristal·lina del domini de la caixa EAGR de MG200 suggereix un rol en interaccions proteïna-proteïna, on aquest domini podria servir de plataforma per interaccions macromoleculars. Ara bé, no s’han descrit defectes estructurals en l’OT de cèl·lules mutants amb delecions en proteïnes que contenen caixes EAGR. Per comprendre millor el paper d'aquest domini, en el segon capítol s'analitza el fenotip de mutants que expressen la proteïna MG200ΔE, que té delecionat específicament el domini de la caixa EAGR.
És interessant que la MG200 és una proteïna multidomini amb homologia amb co-xaperones Hsp40. La interrupció del locus MG_200 per la inserció de transposó dóna lloc a mutants adherents amb un fenotip no mòtil. Una anàlisi recent d'aquests mutants ha evidenciat la presència d'un fragment animo-terminal de MG200, que conté un domini J i una regió rica en glicina i fenilalanina característics de les proteïnes DnaJ. En el tercer capítol, s'ha estudiat la contribució d’aquests dominis amino-terminals de MG200 en la funció general de la proteïna.Mycoplasma genitalium is an emerging sexually transmitted pathogen. This wall-less microorganism is among the smallest, self-replicating cell known. Its streamlined genome is an appealing model of a minimal cell. Behind this apparent simplicity, its cell membrane hides a complex cytoskeleton that shapes and polarizes the cell. In this way, cells show a differentiated tip structure, known as terminal organelle (TO), which is involved in key processes of its parasitic way of life. Moreover, TO is involved in gliding motility. This unique motility mechanism is related in many aspects of the biology of this microorganism, with especial relevance in pathogenesis. Nevertheless, the mechanics behind it is still poorly characterized. The general aim of the present work is to deepen the understanding of this mechanism by studying the specific contribution of different domains of proteins previously related with the TO or motility. TO is organized around an internal cytoskeletal structure. MG218 protein is one of the main proteins of the cytoskeleton and has a central role in the TO formation. Recently, a novel protein that shares the C-terminal sequence of MG218 has been identified. This new protein (MG218-s) is translated from a specific mRNA transcribed from a promoter located inside mg218 gene but the precise translation start remains undefined. To gain insight into the function of this protein, in the first chapter we have addressed the construction of a MG218-s null strain still expressing the MG218 full-length protein. In order to obtain this mutant, a new strategy to introduce point mutations in the M. genitalium genome has been designed. This strategy allowed the identification of the starting Met of the MG218-s protein and the generation of an MG218-s null mutant that helped to understand the role of this new protein. The second and third chapters are focused on the study of the MG200 protein, which is an important element of the motility machinery. MG200 and MG386 protein are the first elements to be related exclusively to motility in M. genitalium. These proteins share common features, being remarkable the presence of a well conserved Enriched in Aromatic and Glycine Residues domain (EAGR box). This domain is exclusively found in mycoplasmas, apparently always in proteins related with motility and/or with the terminal organelle architecture, as MG312. The analysis of the crystal structure of the MG200 EAGR box supported a role in protein-protein interactions, indicating that it can be a platform for interactions with other macromolecules. However, no apparent structural defects in the TO architecture have been shown by mutant cells bearing deletions in proteins containing EAGR boxes. To further understand the role of this domain, the second chapter analyzes the phenotype of mutants that express the MG200 protein bearing a deletion of the EAGR box domain. The observations made suggest that the EAGR boxes would play a relevant and specific role in motility. Interestingly, MG200 is a multi-domain protein that has homology to the Hsp40 co-chaperones. Transposon disruption of MG_200 locus led to adherent strains with a non-motile phenotype. A recent analysis of these mutants has evidenced the presence of an N-terminus MG200 fragment, which contains a J-domain and a glycine and phenylalanine rich region (G/F) characteristic of DnaJ proteins. In the third chapter, we have studied the contribution of the amino-terminal domains of MG200 in its general function.
14
Preuten, Tobias. "Organellar gene expression." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16142.
Full textAbstract:
Zusätzlich zu der eubakteriellen RNA-Polymerase (RNAP) der Plastiden sind im Zellkern von Arabidopsis thaliana drei weitere, phagentypische RNAP kodiert, die jeweils aus nur einer Einheit aufgebaut sind. Die Enzyme RpoTp und RpoTm werden in die Plastiden, bzw. die Mitochondrien transportiert, während RpoTmp in beiden Organellen zu finden ist. Um die Lichtabhängigkeit der RpoT-Gene zu untersuchen, wurde die lichtinduzierte Akkumulation ihrer Transkripte in 7-Tage alten Keimlingen, sowie 3- bzw. 9-Wochen alten Rosettenblättern mittels quantitativer real-time PCR ermittelt. Die entwicklungsabhängige Regulation der RpoT-Transkript-Akkumulation wurde außerdem während der Blattentwicklung analysiert. Zusätzlich wurde der Einfluss des circadianen Rhythmus untersucht. Es stellte sich heraus, dass die Transkriptakkumulation aller drei RpoT-Gene stark lichtinduziert war und nur marginalen circadianen Schwankungen unterlag. In weiteren Versuchen mit verschiedenen Lichtrezeptor-Mutanten und unterschiedlichen Lichtqualitäten wurde der Einfluss multipler Rezeptoren auf den Prozess der Lichtinduktion gezeigt. In den Zellen höherer Pflanzen finden sich drei Genome. Die Biogenese von Chloroplasten und Mitochondrien, sowie lebenswichtige Prozesse, wie Atmung und Photosynthese setzen oftmals die Aktivität von Genen auf mindestens zwei dieser Genome voraus. Eine intrazelluläre Kommunikation zwischen den verschiedenen Genomen ist daher unumgänglich für einen funktionierenden Stoffwechsel der Pflanze. In dieser Arbeit wurde herausgestellt, dass die Zahl mitochondrialer Genkopien in photosynthetisch inaktiven Arabidopsis-Keimlingen drastisch erhöht ist. Bei der Untersuchung des DNA-Gehaltes in Proben, die Altersstufen von 2-Tage alten Keimblättern bis hin zu 37-Tage alten, seneszenten Rosettenblättern umfassten, fand sich ein deutlicher Anstieg der Kopienzahlen in älteren Rosettenblättern. Außerdem unterschieden sich die Kopienzahlen der untersuchten Gene zum Teil erheblich voneinander.In addition to eubacterial-like multi-subunit RNA polymerases (RNAP) localized in plastids and the nucleus, Arabidopsis thaliana contains three phage-like single-unit, nuclear-encoded, organellar RNAPs. The enzymes RpoTp and RpoTm are imported into plastids and mitochondria, respectively, whereas RpoTmp shows dual targeting properties into both organelles. To investigate if expression of the RpoT genes is light-dependent, light-induced transcript accumulation of RpoTm, RpoTp and RpoTmp was analyzed using quantitative real-time-PCR in 7-day-old seedlings as well as in 3- and 9-week-old rosette leaves. To address the question whether RpoT transcript accumulation is regulated differentially during plant development transcript abundance was measured during leaf development. Additionally, effects of the plants circadian rhythm on RpoT transcript accumulation were analyzed. Transcripts of all three RpoT genes were found to be strongly light-induced even in senescent leaves and only marginally influenced by the circadian clock. Further analyses employing different photoreceptor mutants and light qualities revealed the involvement of multiple receptors in the light-induction process. The biogenesis of mitochondria and chloroplasts as well as processes like respiration and photosynthesis require the activity of genes residing in at least two distinct genomes. There have to be ways of intracellular communication between different genomes to control gene activities in response to developmental and metabolic needs of the plant. In this study, it was shown that gene copy numbers drastically increased in photosynthetically inactive Arabidopsis seedlings. Mitochondrial DNA contents in cotyledons and leaves ranging in age from 2-day-old cotyledons to 37-day-old senescent rosette leaves were examined. A common increase in senescing rosette leaves and drastic differences between individual genes were found, revealing the importance of an integrative chondriome in higher plant cells.
15
Ekal, Lakhan. "Characterisation of organelle maintenance in Saccharomyces cerevisiae." Thesis, University of Sheffield, 2018. http://etheses.whiterose.ac.uk/21940/.
Full text
16
Cull, Benjamin. "Autophagy and organelle turnover in Leishmania major." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/4396/.
Full text
17
Dierckxsens, Nicolas. "Targeted organelle genome assembly and heteroplamsy detection." Doctoral thesis, Universite Libre de Bruxelles, 2018. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/277507.
Full textAbstract:
Thanks to the development of next-generation sequencing (NGS) technology, whole genome data can be readily obtained from a variety of samples. Since the massive increase in available sequencing data, the development of efficient assembly algorithms has become the new bottleneck. Almost every new released tool is based on the De Brujin graph method, which focuses on assembling complete datasets with mathematical models. Although the decreasing sequencing costs made whole genome sequencing (WGS) the most straightforward and least laborious approach of gathering sequencing data, many research projects are only interested in the extranuclear genomes. Unfortunately, few of the available tools are specifically designed to efficiently retrieve these extranuclear genomes from WGS datasets. We developed a seed-and-extend algorithm that assembles organelle circular genomes from WGS data, starting from a single short seed sequence. The algorithm has been tested on several new (Gonioctena intermedia and Avicennia marina) and public (Arabidopsis thaliana and Oryza sativa) whole genome Illumina datasets and always outperformed other assemblers in assembly accuracy and contiguity. In our benchmark, NOVOPlasty assembled all genomes in less than 30 minutes with a maximum RAM memory requirement of 16 GB. NOVOPlasty is the only de novo assembler that provides a fast and straightforward manner to extract the extranuclear sequences from WGS data and generates one circular high quality contig.Heteroplasmy, the existence of multiple mitochondrial haplotypes within an individual, has been researched across different fields. Mitochondrial genome polymorphisms have been linked to multiple severe disorders and are of interest to evolutionary studies and forensic science. By utilizing ultra-deep sequencing, it is now possible to uncover previously undiscovered patterns of intra-individual polymorphism. However, it remains challenging to determine its source. Current available software can detect polymorphic sites but are not capable of determining the link between them. We therefore developed a new method to not only detect intra-individual polymorphisms within mitochondrial and chloroplast genomes, but also to look for linkage among polymorphic sites by assembling the sequence around each detected polymorphic site. Our benchmark study shows that this method can detect heteroplasmy more accurately than any method previously available and is the first tool that is able to completely or partially reconstruct the origin sequences for each intra-individual polymorphism.Doctorat en Sciences
info:eu-repo/semantics/nonPublished
18
García, Morales Luis. "Role of MG491 protein in the motile machinery of Mycoplasma genitalium." Doctoral thesis, Universitat Autònoma de Barcelona, 2015. http://hdl.handle.net/10803/328424.
Full textAbstract:
Mycoplasma genitalium és un patogen de transmissió sexual emergent. Aquest bacteri sense paret cel·lular causa uretritis, a més d’altres malalties inflamatòries genitals. Aquest microorganisme utilitza adhesines especialitzades i localitzades a l’organela terminal per adherir-se a cèl·lules hoste i altres superfícies. L’organela terminal, que també es troba involucrada en la motilitat cel·lular, és una estructura polar sostinguda internament per un citosquelet. Al primer capítol, s’ha dissenyat una soca mutant de M. genitalium deficient per a la proteïna MG491 que mostra una desestabilització general de les proteïnes involucrades en l’organització de l’organela terminal. Aquest mutant exhibeix sorprenents similituds amb el mutant defectiu per a la proteïna MG218, aïllat prèviament. Després de la introducció d’una còpia extra d’MG_318 a les dues soques, la quantitat d’adhesines va augmentar considerablement. Aquestes soques es van caracteritzar per microcinematografia, microscòpia de fluorescència i criomicroscòpia electrònica, revelant la presència de cèl·lules i filaments mòtils en absència de diverses proteïnes que s’havien considerat essencials per a l’adhesió i el moviment. Aquests resultats indiquen que els complexes d’adhesines juguen un paper important en la maquinària mòtil de M. genitalium i demostren que el fragment central del citosquelet no és el motor molecular que propulsa les cèl·lules de micoplasma. Aquestes soques contenen una versió minimitzada de la maquinària mòtil aportant un valuós model per investigar les característiques d’adhesió i motilitat d’aquest patogen humà. L’estructura cristal·lina de la proteïna MG491 ha sigut recentment determinada. La molècula de MG491 és un tetràmer que presenta una organització única com a dímer d’heterodímers estructurals. Aquest ordenament asimètric resulta en dos interfases molt diferents, aspecte que explica la formació de només el 50% dels ponts disulfur entre els residus Cys87 de les diferents subunitats. A més, s’ha caracteritzat in vitro la interacció entre el C-terminal de MG491 i el domini EAGR de la proteïna MG200. Al segon capítol, s’han dissenyat soques de M. genitalium amb mutacions en residus clau en l’organització de MG491. Aquestes soques mutants preserven nivells normals de totes les proteïnes de l’organela terminal, però mostren greus alteracions en la motilitat. Els resultats d’aquest treball indiquen que la proteïna MG491 està involucrada en l’estabilitat i ancoratge de l’organela terminal de M. genitalium i juga un paper important en la motilitat cel·lular. Al tercer capítol, s’ha desenvolupat un nou mètode per quantificar la capacitat d’hemadsorció de soques de micoplasma. Aquesta capacitat tradicionalment s’ha investigat determinant la capacitat d’unió d’aquestes cèl·lules a eritròcits i és un tret distintiu àmpliament examinat quan s’estudien soques de micoplasma. Tot i que els protocols per determinar qualitativament l’hemadsorció o hemaglutinació de micoplasmes són molt directes, els mètodes actuals per mesurar de forma quantitativa l’hemadsorció són cars i poc reproduïbles. Utilitzant citometria de flux, s’ha desenvolupat un procediment per quantificar ràpidament i de forma acurada l’activitat d’hemadsorció de soques de micoplasma en presència de SYBR Green I, un fluorocrom vital que tenyeix els àcids nucleics, permetent resoldre els eritròcits i els micoplasmes per mida i fluorescència. Aquest mètode és reproduïble, permet l’anàlisi cinètica de les dades obtingudes i la quantificació precisa de l’hemadsorció basada en paràmetres d’unió estàndards com la constant de dissociació Kd. Aquest procediment pot ser implementat fàcilment en un assaig estàndard per testar l’activitat d’hemadsorció del creixent número d’aïllats clínics i soques mutants de diferents espècies de micoplasma, proporcionant dades valuoses sobre la virulència d’aquests microorganismes.Mycoplasma genitalium is an emerging human sexually transmitted pathogen. This cell-wall less bacterium causes urethritis and other genital inflammatory diseases. This microorganism uses specialized adhesins located at the terminal organelle to adhere to host cells and surfaces. The terminal organelle is a polar structure protruding from the main cell body that is internally supported by a cytoskeleton and also has an important role in cell motility. In the first chapter, we have engineered a M. genitalium null mutant for MG491 protein showing a massive downstream destabilization of proteins involved in the terminal organelle organization. This mutant strain exhibited striking similarities with the previously isolated MG_218 null mutant strain. Upon introduction of an extra copy of MG_318 in both strains, the amount of main adhesins dramatically increased. These strains were characterized by microcinematography, fluorescent microscopy and cryo-electron microcopy, revealing the presence of motile cells and filaments in the absence of many proteins previously considered essential for cell adhesion and motility. These results indicate that adhesin complexes play a major role in the motile machinery of M. genitalium and demonstrate that the rod element of the cytoskeleton core is not the molecular motor propelling mycoplasma cells. These strains containing a minimized version of the motile machinery also provide a valuable cell model to investigate the adhesion and gliding properties of this human pathogen. The crystal structure of M. genitalium MG491 protein has been recently determined. The MG491 molecule is a tetramer presenting a unique organization as a dimer of structural heterodimers. The asymmetric arrangement results in two very different intersubunit interfaces, which can explain the formation of only 50% of the disulphide bridges between Cys87 residues. Furthermore, it has been characterized the in vitro interaction between MG491 C-terminal and the EAGR domain from MG200 protein. In the second chapter, we have engineered M. genitalium cells with mutations in key residues for MG491 organization. These mutants present drastic alterations in motility, while preserving normal levels of terminal organelle proteins. The results in this work indicate that MG491 protein is involved in the stability and anchoring of the M. genitalium terminal organelle and plays a particular role in gliding motility. In the third chapter, we have developed a new method to quantify the hemadsorption activity of mycoplasma strains. Attachment activity of mycoplasma cells has been traditionally investigated by determining their hemadsorption ability to red blood cells and it is a distinctive trait widely examined when characterizing the different mycoplasma species. Despite the fact that protocols to qualitatively determine the hemadsorption or hemagglutination of mycoplasmas are straightforward, current methods when investigating hemadsorption at the quantitative level are expensive and poorly reproducible. By using flow cytometry, we have developed a procedure to quantify rapidly and accurately the hemadsorption activity of mycoplasmas in the presence of SYBR Green I, a vital fluorochrome that stains nucleic acids, allowing resolving erythrocyte and mycoplasma cells by their different size and fluorescence. This method is very reproducible and permits the kinetic analysis of the obtained data and a precise hemadsorption quantification based on standard binding parameters such as the dissociation constant Kd. The procedure we developed could be easily implemented in a standardized assay to test the hemadsorption activity of the growing number of clinical isolates and mutant strains of different mycoplasma species, providing valuable data about the virulence of these microorganisms.
19
Roggan, Jens Stefan. "Heterodinukleare Molybdän/Bismut-Organyle." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2007. http://dx.doi.org/10.18452/15634.
Full textAbstract:
Diese Arbeit handelt von der Synthese und Charakterisierung verschiedener molekularer heterobimetallischer Komplexe der Elemente Molybdän und Bismut. Solche Verbindungen können als Modelle für die bei der SOHIO-Oxidation von Propen zu Acrolein zum Einsatz kommenden heterogenen Bismutmolybdat-Katalysatoren angesehen werden, sie sind aber auch als Einkomponentenvorstufen für die Herstellung funktionaler Materialien von Interesse. In Reaktionen zwischen Molybdocendihydriden und Bismutalkoxiden entstehen Komplexe, in denen Molybdän- und Bismutatome Über Metallbindungen verbunden sind. In einigen dieser Komplexe wurden intramolekulare C-H Aktivierungsprozesse beobachtet, die zur Ausbildung gebogener Bindungen zwischen Bismut- und Cyclopentadienyl-Kohlenstoffatomen führen. Die betreffenden Komplexe wurden unter anderem auch mit quantenchemischen Methoden untersucht. Die Synthese und erstmalige strukturelle Charakterisierung von Verbindungen, in denen Molybdän- und Bismutatome Über Sauerstoffatome verbrückt werden, erfolgte mittels Reaktionen zwischen (NBu4)[Cp*MoO3] (Cp* = Pentamethylcyclopentadienyl) und geeigneten Bismut(V) oder Bismut(III)-Ausgangsstoffen. Einer dieser Komplexe konnte als Einkomponentenvorstufe für die Herstellung von Bismutmolybdat-Nanopartikeln eingesetzt werden, wobei sich das dabei gebildete Material zudem als selektiver Ethanolsensor erwies. Weiterhin werden in der Arbeit die Synthese und Charakterisierung der Bismut(III)-Verbindungen (o-Tolyl)2BiOR (R = H, Me, Bi(o-Tolyl)2) und ihre wechselseitige Umwandlung beschrieben.The unique property of nMoO3/Bi2O3 phases to act as catalysts for the allylic oxidation of propene remains a subject of intense discussion. This as well as the fact that Mo/Bi oxide materials also show other interesting properties e.g. as gas sensors, stimulates research with respect to this element combination also on the molecular level, as described in this thesis. By reactions between molybdocenedihydrides and bismuth alkoxides a series of complexes was synthesised which contain molybdenum-bismuth bonds in a carbonyl-free ligand sphere. Intramolecular C-H activation processes, facilitated by complex induced proximity effects, lead to the formation of bent bonds in some of these complexes. DFT-calculations were performed in order to obtain insight into the bonding situation of these complexes, too. The first complexes with oxygen bridged molybdenum and bismuth atoms were prepared by reactions between (NBu4)[Cp*MoO3] (Cp* = pentamethylcyclopentadienide) and suited bismuth(III) and bismuth(V) reagents. One of these compounds was used as single source precursor for the preparation of bismuthmolybdate nanoparticles in a procedure based on the polyol method. This material proved to be capable of sensing ethanol selectively at elevated temperatures. Additionally, the synthesis and reactions of some bismuth(III) compounds (o-Tolyl)2BiOR (R = H, Me, Bi(o-Tolyl)2) are described in this thesis.
20
Sundström, Isak. "Det beslutande organet." Thesis, Konstfack, Institutionen för Konst (K), 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-2992.
Full textAbstract:
I museet eller i galleriet är distansen uppenbar, saker sätts bakom glas och transformeras till konsteller etnologiska föremål. Men vi skapar också ett eget glas mellan oss själva och omvärlden.Verkligheten är ingenting utan våra fantasier och illusioner. Om verkligheten bara skulle varanågon sorts klar, ofiltrerad verklighet skulle det bli outhärdligt att leva i den. Fantasin bygger vårverklighet. Det ena existerar inte utan det andra. Fantasin är kittet, texturen som gör det kul elleråt minstående uthärdligt att leva.Jag kan nog på ett basalt plan se mitt konstnärskap som en bra anledning till att konstruera,fantisera ihop en verklighet, eller kanske snarare en annan sorts värld som är mer än vad den är.För mig blir nog fantasin som en sorts metod. Ett jobb som tillåter mig att gräva blandfantasifoster och då synligöra en verklighet. Filmer, böcker och konst har för mig oftaegenskapen att antyda konturerna av den verklighet som vi konstruerat och lever i.
21
Dovey, C. L. "The PML-NB : a stress responsive nuclear organelle." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598617.
Full textAbstract:
The Promyelocytic Leukaemia Nuclear Body (PML-NB) is a subnuclear structure disrupted in the cells of PML. Identification of proteins that interact with PML in the NB, before and after stress, will help to define the role of this structure in the cellular response to damage. In this study, changes to the PML-NB were investigated after X-ray-induced DNA damage. Initially the reorganisation of the PML-NB was tracked via immunofluorescence and quantified, at time points after exposure to 1-15Gy, in order to identify the optimum dose and time with which to study changes to the PML-NB composition. At the higher doses of X-ray, the PML-NBs dispersed into more numerous, smaller structures. Gel filtration chromatography was conducted to quantify the decrease in size of the PML-NB after DNA damage, which was observed by immunofluorescence. The majority of the cellular PML was shown to be in large complex, of ³ 4MDa in size prior to insult, and exposure to X-ray resulted in a reduction in the size of the PML complexes. Attempts to isolate the entire PML-NB protein complex by large-scale immunoprecipitation were conduced before, and at time points after irradiation, in order to analyse the composition of the endogenous complex. Two novel potential binding proteins were identified from the immunoprecipitates by mass spectroscopy, one of which, Phospholipase C gamma-1, was investigated further. Responses of the PML-NB to other stresses were also investigated in cells expressing intranuclear inclusions of proteins that contain expanded polyglutamine repeats. These inclusion bodies had previously been reported to sequester PML. It was shown in this study that the normal responses of the PML-NB to various stresses were prevented following sequestration to the inclusion bodies, and following sequestration to other non-pathologic inclusion forming proteins. Further, the DNA damage response proteins BRCA1 and Rad51 showed subnormal responses to damage in cells expressing inclusion bodies.
22
Vaughan, Simon Paul. "Tissue and organelle targeted transgene expression in plants." Thesis, Durham University, 2003. http://etheses.dur.ac.uk/3090/.
Full textAbstract:
This research develops systems for tissue and organelle targeted transgene expression in commercial strawberry. Two approaches were taken to achieve this. I) the development of a root-specific expression system for nuclear transgenes and II) the development of plastid transformation methodologies for strawberry. FavRB7, a root-specific, RB7 tonoplast intrinsic protein homologue was isolated from strawberry root tissue using reverse transcnptase-polymerase chain reaction (RT-PCR). A 2.8 kb region of promoter sequence associated with the FavRB7 gene was isolated and cloned upstream of the β-glucuronidase (gusA) reporter gene. Transgenic strawberry and tobacco were produced harbouring the gusA gene under control of the FavRB7 gene promoter. Promoter activity in these lines was characterised using histochemical, fluorometric and RNA molecular assays. FavRB7 promoter activity was shown to be strong and near root-specific in strawberry. However in contrast, in the heterologous species tobacco, FavRB7 promoter activity was shown to be constitutive. A series of novel plastid transformation vectors, harbouring selectable and scorable marker genes under control of strawberry plastome regulatory elements, were created to enable plastid transformation in strawberry and tobacco. Plastid transformation methodologies were developed for strawberry utilising the aminoglycoside 3'-adenyltransferase (aadA) gene and spectinomycin selection. Additionally, plastid transformation using a non-antibiotic selection system was investigated utilising the xylose isomerase (xylA) gene and D-xylose selection. Heteroplasmic plastid transformed strawberry lines were recovered using both selection systems and expression of the soluble modified green fluorescent protein (smGFP) scorable marker gene visualised specifically in strawberry chloroplasts of three individual lines. Additionally, heteroplasmic plastid transformed tobacco lines harbouring transgenes under control of strawberry plastome regulatory elements were generated using spectinomycin selection.
23
Duch, Anna Marta. "In organello studies of mammalian mitochondrial DNA replication." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648093.
Full text
24
Voiglio, Eric Joseph. "Conservation aérobie des organes : développement d'un modèle de bloc multi-viscéral pour l'étude d'une émulsion de fluorocarbure." Lyon 1, 2000. http://www.theses.fr/2000LYO1T124.
Full text
25
Paap, Joachim. "Neuartige p-Organyle [pi-Organyle] der schweren Alkalimetalle und des Magnesiums Synthese und Festkörperstrukturen /." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97280689X.
Full text
26
Mayer, Jürgen. "Investigation of the biophysical basis for cell organelle morphology." Master's thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-26600.
Full textAbstract:
It is known that fission yeast Schizosaccharomyces pombe maintains its nuclear envelope during mitosis and it undergoes an interesting shape change during cell division - from a spherical via an ellipsoidal and a peanut-like to a dumb-bell shape. However, the biomechanical system behind this amazing transformation is still not understood. What we know is, that the shape must change due to forces acting on the membrane surrounding the nucleus and the microtubule based mitotic spindle is thought to play a key role. To estimate the locations and directions of the forces, the shape of the nucleus was recorded by confocal light microscopy. But such data is often inhomogeneously labeled with gaps in the boundary, making classical segmentation impractical. In order to accurately determine the shape we developed a global parametric shape description method, based on a Fourier coordinate expansion. The method implicitly assumes a closed and smooth surface. We will calculate the geometrical properties of the 2-dimensional shape and extend it to 3-dimensional properties, assuming rotational symmetry.
Using a mechanical model for the lipid bilayer and the so called Helfrich-Canham free energy we want to calculate the minimum energy shape while respecting system-specific constraints to the surface and the enclosed volume. Comparing it with the observed shape leads to the forces. This provides the needed research tools to study forces based on images.
27
Sengupta, Debrup. "Mechanism of GRASP65 Mediated Organelle Tethering and its Regulation." Research Showcase @ CMU, 2010. http://repository.cmu.edu/dissertations/13.
Full textAbstract:
In higher eukaryotes, the Golgi apparatus is organized into a single copy, ribbon-like membrane network. The ribbon-like structure is established by lateral homotypic interactions between analogous cisternae in adjacent ministacks. Lateral linking may involve homotypic tethering followed by membrane fusion. Indeed, ribbon formation is blocked by depletion of the membrane tethering proteins GRASP65 and GRASP55, which are localized to cis and medial Golgi cisternae, respectively. In this thesis we present a structure-function analysis of GRASP65 in order to further understand its mechanism of action and regulation in Golgi ribbon formation. Because GRASP65 homo-oligomerizes in vitro we hypothesized that its self-interaction links cis cisternae prior to fusion. To test this model and determine the mechanism of GRASP65 selfinteraction we developed a cell-based organelle-tethering assay. GRASP65 was targeted to the mitochondrial outer membrane allowing a quantitative visual assessment of induced mitochondrial tethering. We observed that GRASP65 interacts in trans to tether organellar membranes, and the tethering involves the binding groove of the first of two PDZ-like domains present at its N-terminus. Tethering also required membrane anchoring of the PDZ domain suggesting a mechanism that orientates the PDZ binding groove to favor interactions in trans. These results identify a homotypic PDZ interaction mediating organelle tethering in living cells. GRASP65 self-interaction is regulated by mitotic phosphorylation but the mechanism is unclear. In fact, the known GRASP65 phosphorylation sites are outside the self-interacting N-terminal domain, and their mutation to mimic phosphorylation failed to block tethering. We identified a site phosphorylated by Polo-like kinase 1 (PLK1) in the GRASP65 N-terminal domain for which mutation to aspartic acid blocked tethering and alanine substitution prevented mitotic Golgi unlinking. Further, using interaction assays, we discovered an internal PDZ ligand adjacent to the PLK phosphorylation site that was required for tethering. These results reveal the mechanism of phospho-inhibition as direct inhibition by PLK1 of the PDZ ligand underlying the GRASP65 self-interaction.
28
Mayer, Jürgen. "Investigation of the biophysical basis for cell organelle morphology." Master's thesis, Max-Planck-Institut für Molekulare Zellbiologie und Genetik, 2008. https://tud.qucosa.de/id/qucosa%3A25225.
Full textAbstract:
It is known that fission yeast Schizosaccharomyces pombe maintains its nuclear envelope during mitosis and it undergoes an interesting shape change during cell division - from a spherical via an ellipsoidal and a peanut-like to a dumb-bell shape. However, the biomechanical system behind this amazing transformation is still not understood. What we know is, that the shape must change due to forces acting on the membrane surrounding the nucleus and the microtubule based mitotic spindle is thought to play a key role. To estimate the locations and directions of the forces, the shape of the nucleus was recorded by confocal light microscopy. But such data is often inhomogeneously labeled with gaps in the boundary, making classical segmentation impractical. In order to accurately determine the shape we developed a global parametric shape description method, based on a Fourier coordinate expansion. The method implicitly assumes a closed and smooth surface. We will calculate the geometrical properties of the 2-dimensional shape and extend it to 3-dimensional properties, assuming rotational symmetry.
Using a mechanical model for the lipid bilayer and the so called Helfrich-Canham free energy we want to calculate the minimum energy shape while respecting system-specific constraints to the surface and the enclosed volume. Comparing it with the observed shape leads to the forces. This provides the needed research tools to study forces based on images.
29
Fujisawa, Alma. "Development of chemical labeling methods for organelle molecule analysis." Kyoto University, 2019. http://hdl.handle.net/2433/243315.
Full text
30
Pereyra, Leonardo Gabriel [Verfasser]. "Organelle dysfunction modulates cholesterol biosynthesis pathway / Leonardo Gabriel Pereyra." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1218299150/34.
Full text
31
Hassan, Hanna. "Proteomic profiling of vesicular organelles." Thesis, KTH, Skolan för bioteknologi (BIO), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-215028.
Full text
32
Schreurs-Engelaar, Maria Elisabeth. "Organen van de coöperatie /." Deventer : Kluwer, 1995. http://www.gbv.de/dms/spk/sbb/recht/toc/272309281.pdf.
Full text
33
Batellier, Jean. "Le prelevement multi-organes." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR1M171.
Full text
34
Richter, Uwe. "Analysis of phage-type RNA polymerase driven transcription in Physcomitrella patens and Arabidopsis." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2014. http://dx.doi.org/10.18452/16886.
Full textAbstract:
In der vorliegenden Arbeit wurde der spezifische Einfluss verschiedener kernkodierter phagentypischer RNA Polymerasen auf die organelläre Genexpression in Physcomitrella und die mitochondrial Genexpression in Arabidopsis untersucht. Während das Fehlen von AtRpoTm in Arabidopsis und PpRpoTmp1 in P. patens lethal ist, wurden Insertionsmutanten für PpRpoTmp2 und AtRpoTmp einer detailierten Untersuchung unterzogen. Sowohl PprpoTmp2, als auch AtrpoTmp Pflanzen zeigten Abweichungen im Phänotyp charakteristisch für mitochondriale Dysfunktion. Identifizierte organelläre Promotoren in P. patens wurden zum Nachweis der Transkriptionsaktivität von PpRpoTmp1 und PpRpoTmp2 in vitro herangezogen. Beide Proteine besitzen die inhärente Fähigkeit zur Promotorerkennung ohne zusätzliche Kofaktoren. Die hier vorgestellten Studien unterstreichen die essentielle Bedeutung von AtRpoTm und PpRpoTmp1 für die Transkription mitochondrialer bzw organellärer Gene in Arabidopsis und P. patens. Im Gegensatz dazu können die Funktionen von AtRpoTmp und PpRpoTmp2 partiell durch andere organelläre RNAPs ersetzt werden. Phänotypische Abweichungen belegen jedoch, das AtRpoTmp und PpRpoTmp2 für die normale Entwicklung von Arabidosis bzw. P. patens essentiell sind. Veränderte Transkriptmengen in AtrpoTmp Pflanzen korrelierten mit genspezifischen Änderungen in der mitochondrialen Transkription. AtRpoTmp muss daher als essentiel für die normale Expression eines spezifischen Sets mitochondrialer Gene angesehen werden. Jedoch konnten für diese mitochondrialer Gene keine AtRpoTmp spezifischen Promotormotive mit reduzierter Aktivität identifiziert werden. Initiationsraten an allen Promotoren stromaufwärts von mitochondrialen Genen mit geringeren Transkriptmengen sind jedoch reduziert. Es erscheint daher wahrscheinlich, daß für einen Teil der mitochondrialen Gene genspezifische Elemente existieren, welche die Transkription durch AtRpoTmp dirigieren.This study aimed to elucidate how the different transcriptional activities are facilitated in mitochondria of Arabidopsis thaliana and in both organelles of Physcomitrella patens. Insertional mutants for PprpoTmp2 and AtrpoTmp were analysed in detail. As for Arabidopsis RpoTm, knock-out of Physcomitrella RpoTmp1 was found to be lethal. Null mutant plants PprpoTmp2 and AtrpoTmp show surprisingly similar but clearly convergent phenotypical aberrations reminiscent of phenotypes reported for other mitochondrial mutants. Evidence is provided that PpRpoTmp1 and PpRpoTmp2 are functional RNA polymerases, which both posses the inherent ability to recognize organellar promoters in a minimal in vitro transcription system without the aid of additional cofactors. The data suggest that coding for two RpoT proteins one representing an enzyme with a high portion of non-specific transcriptional activity, as seen for AtRpoTmp and PpRpoTmp1 and one that can act as a single-polypeptide enzyme and recognize numerous mitochondrial promoters in vitro as AtRpoTm and PpRpoTmp2 echo convergent inventions but reflect complementing roles of these RNA polymerases in plant mitochondrial transcription. Phenotypical aberrations of rpoTmp2 plants suggest RpoTmp2 is important for normal growth and development. Altered transcript levels in AtrpoTmp were found to result from gene-specific transcriptional changes, establishing that AtRpoTmp functions in distinct transcriptional processes within mitochondria. Decreased transcription of a specific set of mitochondrial genes in AtrpoTmp was not associated with changes in the utilisation of specific promoters. Therefore AtRpoTmp function is not promoter-specific but gene-specific. This indicates that additional gene-specific elements direct the transcription of a subset of mitochondrial genes by RpoTmp.
35
Chao, Jesse Tzu-Cheng. "The endoplasmic reticulum diffusion barrier and inter-organelle contact sites." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/45256.
Full text
36
Dolman, Nicholas James. "Polarised signalling and organelle distribution in the pancreatic acinar cell." Thesis, University of Liverpool, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406669.
Full text
37
Hickey, Patrick C. "Imaging vesicle trafficking and organelle dynamics in living fungal hyphae." Thesis, University of Edinburgh, 2001. http://hdl.handle.net/1842/12078.
Full textAbstract:
The aims of the research were to develop and apply live cell imaging techniques using confocal microscopy to image and analyse vesicle trafficking, organelle dynamics and molecular localization at high spatial and temporal resolution in filamentous fungi. The dyes FM4-64, and to a lesser extent, FM1-43, were used to analyse vesicle trafficking, and as general probes to image hyphal tip growth, branching, septum formation, hyphal fusion, conidiophore development and the early stages of protoperithecium development. Neurospora crassa was the main system studied, although a taxonomically diverse range of other species were also analysed. Uptake of FM4-64 into hyphae was shown to be time- and energy-dependent, consistent with internalization being mediated by endocytosis. FM4-64 proved to be an excellent vital stain for different cell components including the apical vesicle cluster (AVC), vacuolar network and mitochondria. The dye was used to compare the morphology of the AVC in 15 different species. The green fluorescent protein (GFP) is a recombinant fluorescent probe that can be targeted to organelles and used to label specific proteins within living cells. Confocal microscopy was used to image transformants of Aspergillus nidulans expressing GFP targeted to the vacuolar network, mitochondria, nuclei, spindle pole bodies, endoplasmic reticulum, and Golgi cistemae. In addition, a range of vital fluorescent dyes were used to image organelles, and results from these studies were compared to those obtained with GFP transformants. Vacuolar staining revealed an extensive tubular network in actively growing regions of hyphae, whereas in sub-apical regions large spherical vacuoles were observed. Division of a single mitochondrion was imaged in Aspergillus nidulans expressing GFP targeted to mitochondria. Potentiometric dyes (especially rhodamine-123) indicated that the membrane potential of mitochondria in growing hyphal tips was higher than that in sub-apical regions.
38
Meeusen, Shelly Lyn. "Analysis of the machinery regulating mitochondrial organelle and genome dynamics /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.
Full text
39
Gavelis, Gregory S. "Evolution of complex organelles in dinoflagellates." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/56291.
Full textAbstract:
Dinoflagellates are an abundant and diverse group of aquatic eukaryotes, with members that have photosynthetic, heterotrophic, or mixotrophic life strategies, as well as a number of unique cytological features. My thesis focuses on two groups of closely related dinoflagellates: polykrikoids and warnowiids. Both include heterotrophic as well as plastid-bearing members, though the number of times photosynthesis has been lost (or gained) in each group is unclear, and the presence and provenance of plastids in some species (e.g., Nematodinium sp. and Polykrikos lebouriae) have been debated. Polykrikoids and warnowiids also contain some of the most complex subcellular structures described--such as nematocysts and, in warnowiids, eye-like ocelloids. Yet these groups are rare in nature and uncultivated, and as such, the origins of their complex organelles are unclear. For my thesis, I modified existing techniques for use on single-cell environmental isolates, and applied these techniques to wild polykrikoid and warnowiid cells. By exploiting the common splice leader sequence found on dinoflagellate transcripts, I was able to amplify a single-cell transcriptome from Polykrikos lebouriae—a dinoflagellate with aberrant plastids. Coupled with single-cell genomics using multiple displacement amplification (MDA), I demonstrated that Polykrikos lebouriae has retained peridinin-type plastids, while photosynthesis has been lost in multiple other polykrikoid species independently. Using MDA and single-cell transmission electron microscopy, I also determined that the eye-like ocelloid of Nematodinium sp. is made in part from a peridinin plastid, and also from mitochondria. Specifically, single-cell focused ion beam scanning electron microscopy (FIB-SEM) allowed me to demonstrate that a retina-like portion of the ocelloid is a small part of a much larger peridinin-plastid that ramifies throughout the Nematodinium cell. Lastly, I investigated the evolution of nematocysts in Polykrikos spp. and Nematodinium sp. using a combination of transcriptomics, TEM, SEM, and FIB-SEM, and inferred that “nematocysts” in these groups evolved independently from those in cnidarians. Thus, nematocyst-like extrusive organelles appear to have evolved multiple times in eukaryotes. The data presented in this thesis show how extreme subcellular complexity has evolved in dinoflagellates through both endosymbiotic and autogenous origins.Science, Faculty of
Zoology, Department of
Graduate
40
Job, Christy Amelia. "The biogenesis of regulated secretory organelles." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309240.
Full text
41
Bassham, Diane C. "The proteolytic processing of organellar proteins." Thesis, University of Warwick, 1993. http://wrap.warwick.ac.uk/102665/.
Full textAbstract:
Biogenesis of the chloroplast involves the activities of both the nuclear and chloroplast genomes. Nuclear-encoded chloroplast proteins are synthesised on free ribosomes in the cytosol and imported into the chloroplast post-translationally. Protein uptake is directed by an N-terminal presequence which is removed after import by specific processing peptidases. Thylakoid lumen proteins must cross three membranes to reach their site of function and have a composite presequence to enable their correct localisation, consisting of an envelope transit domain (ETD) and a thylakoid transfer domain (TTD). The ETD is removed after translocation across the envelope by a stromal processing peptidase (SPP) to produce an intermediate-sized protein and the TTD then allows transport into the thylakoid lumen where it is removed by a thylakoidal processing peptidase (TPP). SPP is a soluble, stromal- located metallopeptidase which also removes the presequences from stromal proteins. Despite its apparently high specificity, there is very little sequence similarity around SPP cleavage sites in stromal and thylakoidal proteins, and little is known about the structural features which SPP recognises. SPP was partially purified from pea chloroplasts and the SPP cleavage site within the presequences of three thylakoid lumen proteins was determined by radiosequencing of the cleavage products with the two-fold aim of identifying the residues around the cleavage site which SPP may recognise and of delineating the ETD and TTD regions of the presequences, allowing their comparison with signal sequences. This information was then used to create four mutant thylakoid lumen precursor proteins by site-directed mutagenesis with altered SPP processing sites. The four mutant proteins all showed a reduced rate and efficiency of processing in an organelle-free time course assay, although all were imported into chloroplasts, correctly localised and processed to the mature size in an in vitro assay. Although SPP has usually been considered to be highly specific for impoited chloroplast precursor proteins, it has recently been suggested that it may also process mitochondrial precursor proteins, which are cleaved in vivo and in vitro to the mature size by the mitochondrial processing peptidase (MPP). These two peptidases were therefore compared in terms of substrate specificity and mechanism by assaying the activity of the two enzymes against chloroplast and mitochondrial precursor proteins. MPP did not process any of the chloroplast precursor proteins available, suggesting that this enzyme is indeed highly specific for mitochondrial precursor proteins. A partially-purified SPP preparation cleaved the mitochondrial precursor proteins tested to a smaller size; however, the site of cleavage in at least some of these proteins was different to the authentic MPP cleavage site, with the stromal preparation cleaving N- terminal to MPP. This stromal processing activity was not due to mitochondrial contamination and evidence from inhibitor sensitivities and column chromatography suggests that SPP cleaves both chloroplast and mitochondrial precursor proteins. Hie SPP processing site within the presequences of two mitochondrial proteins was determined by radiosequencing and compared with the chloroplast cleavage sites, and this data should enable further analysis to determine the features which constitute a site which can be recognised by SPP. An SPP activity was also partially-purified from Chlamydomonas reinhardtii and shown to be located in the stroma. This activity is able to process chloroplast precursor proteins from C. reinhardtii and pea to an intermediate or mature size, emphasising the similarity of the specificity of SPP from these two species.
42
Munoz, Víctor Hugo Anaya. "A theoretical model on the role of lateral gene transfer in the evolution of endosymbiotic genomes." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16446.
Full textAbstract:
Laterale Gentransfer wurde zuerst von Schwartz und Dayhoff (1978) entdeckt, die es aber als eine Exzentrizität werteten und als solche ignorierten. Später, als mehrere DNS- und Eiweißsequenzen sequenziert und raffiniertere Phylogenien rekonstruiert wurden, hat die Rolle an Relevanz gewonnen, die der laterale (oder horizontale) Gentransfer in der evolutionären Geschichte von lebendigen Organismen gespielt hat. Außerdem existiert auch zwischen Endosymbionten und Zellkernen statt. Ich habe ein theoretisches Modell entwickelt, das den lateralen Gentransfer zwischen Endosymbionten und dem Zellkern repräsentiert. Das Modell erforscht die Bedeutung des Fehlens von Rekombination in den Organellen (Muller’s Ratchet) sowie Abweichungen von Muller’s Ratchet in Form der non-symmetrical homologous recombination in Gentransfermechanismen. Ich habe zum einen Zellkern-Inkompatibilitäten, die aus der Übertragung eines Gens resultieren, und zum anderen Zyto- und Zellkern-Inkompatibilitäten zwischen den mutierten endosymbiotischen Genomen und dem modifizierten Zellenkern untersucht. Die Ergebnisse zeigen, dass unter bestimmten Bedingungen die Existenz oder Nicht-Existenz von Rekombination die gleiche Wirkung haben können. Es zeigte sich auch, dass Rekombination, wenn sie vorkommt und wenn sie nicht symmetrisch ist, starke Auswirkungen auf die Allelenfrequenz einer Population haben kann. Es wurde auch klar, dass es eine starke Beziehung zwischen dem Zellkern und endosymbiotischen Genomen gibt, und dass das evolutionäre Schicksal des einen größtenteils von den evolutionären Kräften abhängig ist, die das andere beeinflussen. Wenn man Zellkern- und Cyto-Zellkerninkompatibilitäten in das Modell einführt, dann zeigen die Ergebnisse, dass die Inkompatibilitäten, die der laterale Gentransfer produziert hat, möglicherweise eine ähnliche Rolle im Speziationsmechanismus spielen könnten wie die Inkompatibilitäten zwischen Mitochondrien und Zellkernen in verschiedenen Nasonia-Arten.Lateral gene transfer has played a key role in the evolution of living beings. This process was first acknowledged in 1978 by Schwartz and Dayhoff but considered a relatively infrequent eccentricity and ignored. Later on, as DNA and protein sequences accumulated and more refined phylogenies were reconstructed, the contribution of lateral (or horizontal) gene transfer to the evolutionary history of living organisms gained relevance. Besides, gene transfer is known to occur not only between independent organisms but also, and more frequently between endosymbionts including eukaryotic organelles. I developed a theoretical model to study the lateral gene transfer process between cell organelles (but extendible to other endosymbionts) and the cell nucleus. The model explores the role of the lack of recombination in the organelles (Muller''s ratchet) as well as deviations from Muller''s ratchet in the form of non-symmetrical homologous recombination in relation with the gene transfer process. Also, nuclear incompatibilities resulting from the inclusion of a transferred gene, and cyto-nuclear incompatibilities between the mutant endosymbiotic genomes and the modified nuclear genome are investigated. The results obtained show that under certain circumstances the existence recombination or its non-existence produce the same results, and that deviations from symmetry in the recombination process might have important effects on the frequency of different alleles. It is also clear that there is a strong relation between nuclear and endosymbiotic genomes, and that the evolutionary fate of one largely depends on the forces affecting the other. When nuclear and cyto-nuclear incompatibilities are introduced in the model, the results show that lateral gene transfer-induced incompatibilities could potentially play a role in the speciation process similar to the one produced by mitochondria in the Nasonia species.
43
Leys, Sally P. "Cytoskeletal architecture, organelle transport, and impulse conduction in hexactinellid sponge syncytia." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0007/NQ32755.pdf.
Full text
44
Reynolds, Deborah Fidelis. "A study of organelle Ca²⺠dynamics in cardiac muscle." Thesis, University of Glasgow, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274262.
Full text
45
Ichas, François. "La mitochondrie, organelle excitable : participation active à la signalisation calcique intracellulaire." Bordeaux 2, 1997. http://www.theses.fr/1997BOR28475.
Full text
46
Jurkovic, Dominika Angelika. "Structure, Organization, and Function of the Terminal Organelle in Mycoplasma penetrans." Miami University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=miami1346776623.
Full text
47
Sellers, Charles Grier. "Feeding, Dark Survival, and Foreign Organelle Retention in an Antarctic Dinoflagellate." Diss., Temple University Libraries, 2014. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/292726.
Full textAbstract:
BiologyPh.D.
The retention by protists of foreign plastids and other organelles obtained from algal prey is an ecologically important example of mixotrophy and also represents a potential pathway for the symbiogenetic evolution of novel permanent plastids. A gymnodinoid dinoflagellate isolated from the Ross Sea, Antarctica (RSD) retains plastids from its haptophyte prey Phaeocystis antarctica. It is a member of the Kareniaceae, a dinoflagellate family whose other members all contain permanent tertiary plastids of haptophyte origin. A subset of its cells also contain foreign nuclei. The following chapters describe experiments that indicate the RSD's selectivity for P. antarctica in feeding and plastid uptake, when compared to other potential prey; and observations that demonstrate survival of plastid-retaining RSD for over two years in the absence of its prey. Further experiments assess the resilience of P. antarctica and the RSD in response to the prolonged darkness of the austral winter.
Temple University--Theses
48
Schiller, Carsten. "s-Organyle [Sigma-Organyle] und p-Komplexe [Pi-Komplexe]: Beiträge zur Organometallchemie der Platinelemente Pt, Rh und Ir." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=969898215.
Full text
49
Buckova, Ivana. "Organe der Tschechischen Aktiengesellschaft." WU Vienna University of Economics and Business, 1999. http://epub.wu.ac.at/3387/1/ap059.pdf.
Full text
50
Schweiger, Regina. "Post-translational preprotein targeting to plant organelles." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-162549.
Full text