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Journal articles on the topic 'Organotypic brain slice'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Humpel, Christian. "Organotypic Brain Slices of ADULT Transgenic Mice: A Tool to Study Alzheimer’s Disease." Current Alzheimer Research 16, no. 2 (2019): 172–81. http://dx.doi.org/10.2174/1567205016666181212153138.

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Transgenic mice have been extensively used to study the Alzheimer pathology. In order to reduce, refine and replace (3Rs) the number of animals, ex vivo cultures are used and optimized. Organotypic brain slices are the most potent ex vivo slice culture models, keeping the 3-dimensional structure of the brain and being closest to the in vivo situation. Organotypic brain slice cultures have been used for many decades but were mainly prepared from postnatal (day 8-10) old rats or mice. More recent work (including our lab) now aims to culture organotypic brain slices from adult mice including tran

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Humpel, Christian. "Organotypic Brain Slice Cultures." Current Protocols in Immunology 123, no. 1 (2018): e59. http://dx.doi.org/10.1002/cpim.59.

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Tsupykov, O., I. Lushnikova, Y. Nikandrova, et al. "A novel model of periventricular leukomalacia on mouse organotypic brain slice culture." Cell and Organ Transplantology 4, no. 2 (2016): 188–93. http://dx.doi.org/10.22494/cot.v4i2.60.

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The creation of adequate in vitro and in vivo models of neural tissue injury is essential to assess the therapeutic effect of pharmacological agents and regenerative potential of various types of stem cells in diseases of the central nervous system. The aim of this work was to create a novel model of cerebral white matter lesions – periventricular leukomalacia (PVL) – on murine organotypic brain slice culture.Materials and methods. The PVL model was developed on cultured organotypic mice brain slices subjected to oxygen-glucose deprivation (OGD) followed by addition of endotoxin lipopolysaccha

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Humpel, C. "Organotypic brain slice cultures: A review." Neuroscience 305 (October 2015): 86–98. http://dx.doi.org/10.1016/j.neuroscience.2015.07.086.

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Joost, Sarah, Stefan Mikkat, Michael Wille, Antje Schümann, and Oliver Schmitt. "Membrane Protein Identification in Rodent Brain Tissue Samples and Acute Brain Slices." Cells 8, no. 5 (2019): 423. http://dx.doi.org/10.3390/cells8050423.

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Acute brain slices are a sample format for electrophysiology, disease modeling, and organotypic cultures. Proteome analyses based on mass spectrometric measurements are seldom used on acute slices, although they offer high-content protein analyses and explorative approaches. In neuroscience, membrane proteins are of special interest for proteome-based analysis as they are necessary for metabolic, electrical, and signaling functions, including myelin maintenance and regeneration. A previously published protocol for the enrichment of plasma membrane proteins based on aqueous two-phase polymer sy

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Phillips, Wiktor S., Mikkel Herly, Christopher A. Del Negro, and Jens C. Rekling. "Organotypic slice cultures containing the preBötzinger complex generate respiratory-like rhythms." Journal of Neurophysiology 115, no. 2 (2016): 1063–70. http://dx.doi.org/10.1152/jn.00904.2015.

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Study of acute brain stem slice preparations in vitro has advanced our understanding of the cellular and synaptic mechanisms of respiratory rhythm generation, but their inherent limitations preclude long-term manipulation and recording experiments. In the current study, we have developed an organotypic slice culture preparation containing the preBötzinger complex (preBötC), the core inspiratory rhythm generator of the ventrolateral brain stem. We measured bilateral synchronous network oscillations, using calcium-sensitive fluorescent dyes, in both ventrolateral (presumably the preBötC) and dor

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Daza, R. A. M., C. Englund, and R. F. Hevner. "Organotypic Slice Culture of Embryonic Brain Tissue." Cold Spring Harbor Protocols 2007, no. 24 (2007): pdb.prot4914. http://dx.doi.org/10.1101/pdb.prot4914.

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8

Ucar, Buket, Sedef Yusufogullari, and Christian Humpel. "Collagen hydrogels loaded with fibroblast growth factor-2 as a bridge to repair brain vessels in organotypic brain slices." Experimental Brain Research 238, no. 11 (2020): 2521–29. http://dx.doi.org/10.1007/s00221-020-05907-7.

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Abstract Vessel damage is a general pathological process in many neurodegenerative disorders, as well as spinal cord injury, stroke, or trauma. Biomaterials can present novel tools to repair and regenerate damaged vessels. The aim of the present study is to test collagen hydrogels loaded with different angiogenic factors to study vessel repair in organotypic brain slice cultures. In the experimental set up I, we made a cut on the organotypic brain slice and tested re-growth of laminin + vessels. In the experimental set up II, we cultured two half brain slices with a gap with a collagen hydroge

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GIANINAZZI, C., M. SCHILD, N. MÜLLER, et al. "Organotypic slice cultures from rat brain tissue: a new approach forNaegleria fowleriCNS infectionin vitro." Parasitology 132, no. 6 (2005): 797–804. http://dx.doi.org/10.1017/s0031182005008619.

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The free-living amoebaNaegleria fowleriis the aetiological agent of primary amoebic meningoencephalitis (PAM), a disease leading to death in the vast majority of cases. In patients suffering from PAM, and in corresponding animal models, the brain undergoes a massive inflammatory response, followed by haemorrhage and severe tissue necrosis. Both,in vivoandin vitromodels are currently being used to study PAM infection. However, animal models may pose ethical issues, are dependent upon availability of specific infrastructural facilities, and are time-consuming and costly. Conversely, cell culture

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Croft, Cara L., and Wendy Noble. "Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease." F1000Research 7 (May 15, 2018): 592. http://dx.doi.org/10.12688/f1000research.14500.1.

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Alzheimer's disease, the most common cause of dementia, is a progressive neurodegenerative disorder characterised by amyloid-beta deposits in extracellular plaques, intracellular neurofibrillary tangles of aggregated tau, synaptic dysfunction and neuronal death. There are no cures for AD and current medications only alleviate some disease symptoms. Transgenic rodent models to study Alzheimer’s mimic features of human disease such as age-dependent accumulation of abnormal beta-amyloid and tau, synaptic dysfunction, cognitive deficits and neurodegeneration. These models have proven vital for imp

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Croft, Cara L., and Wendy Noble. "Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease." F1000Research 7 (June 27, 2018): 592. http://dx.doi.org/10.12688/f1000research.14500.2.

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Alzheimer's disease, the most common cause of dementia, is a progressive neurodegenerative disorder characterised by amyloid-beta deposits in extracellular plaques, intracellular neurofibrillary tangles of aggregated tau, synaptic dysfunction and neuronal death. Transgenic rodent models to study Alzheimer’s mimic features of human disease such as age-dependent accumulation of abnormal beta-amyloid and tau, synaptic dysfunction, cognitive deficits and neurodegeneration. These models have proven vital for improving our understanding of the molecular mechanisms underlying AD and for identifying p

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Wilhelmi, Eckbert, Ulrich H. Schöder, Akilah Benabdallah, Frank Sieg, Jörg Breder, and Klaus G. Reymann. "Organotypic Brain-slice Cultures from Adult Rats: Approaches for a Prolonged Culture Time." Alternatives to Laboratory Animals 30, no. 3 (2002): 275–83. http://dx.doi.org/10.1177/026119290203000304.

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Animal experiments are widely used in neurobiological and neuropharmacological research. Today, juvenile brain organotypic slice cultures have partially replaced in vivo experiments, but there is no adequate in vitro counterpart for the adult brain. The present study was aimed at the long-term culture of physiologically intact hippocampal slices from adult rats, by improving the conditions for preparation and culture, and the development of a new culture medium. A cerebrospinal fluid (CSF)-like medium was used, which was modified with a variety of supplements, including energy precursors, free

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Spahr-Schopfer, Isabelle, Lazlo Vutskits, Nicholas Toni, Pierre-Alain Buchs, Lorena Parisi, and Dominique Muller. "Differential Neurotoxic Effects of Propofol on Dissociated Cortical Cells and Organotypic Hippocampal Cultures." Anesthesiology 92, no. 5 (2000): 1408–17. http://dx.doi.org/10.1097/00000542-200005000-00032.

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Background Propofol is a widely used anesthetic agent for adults and children. Although extensive clinical use has demonstrated its safety, neurologic dysfunctions have been described after the use of this agent. A recent study on a model of aggregating cell cultures reported that propofol might cause irreversible lesions of gamma-aminobutyric acid-mediated (GABAergic) neurons when administered at a critical phase of brain development. We investigated this issue by comparing the effects of long-term propofol treatment on two models of brain cultures: dissociated neonatal cortical cell cultures

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Roux, Amandine, Xinhe Wang, Katelyn Becker та Jiyan Ma. "Modeling α-Synucleinopathy in Organotypic Brain Slice Culture with Preformed α-Synuclein Amyloid Fibrils". Journal of Parkinson's Disease 10, № 4 (2020): 1397–410. http://dx.doi.org/10.3233/jpd-202026.

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Background: Synucleinopathy is a group of neurodegenerative disorders characterized by neurodegeneration and accumulation of alpha-synuclein (α-syn) aggregates in various brain regions. The detailed mechanism of α-syn-caused neurotoxicity remains obscure, which is partly due to the lack of a suitable model that retains the in vivo three-dimensional cellular network and allows a convenient dissection of the neurotoxic pathways. Recent studies revealed that the pre-formed recombinant α-syn amyloid fibrils (PFFs) induce a robust accumulation of pathogenic α-syn species in cultured cells and anima

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Back, Adam, Kelsey Y. Tupper, Tao Bai, et al. "Ammonia-induced brain swelling and neurotoxicity in an organotypic slice model." Neurological Research 33, no. 10 (2011): 1100–1108. http://dx.doi.org/10.1179/1743132811y.0000000046.

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Thomas, Mark P., Margaret I. Davis, Daniel T. Monaghan, and Richard A. Morrisett. "Organotypic Brain Slice Cultures for Functional Analysis of Alcohol-Related Disorders." Alcoholism: Clinical & Experimental Research 22, no. 1 (1998): 51. http://dx.doi.org/10.1097/00000374-199802000-00006.

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Falsig, Jeppe. "S5-01-06: Prion-induced neurodegeneration in organotypic brain slice cultures." Alzheimer's & Dementia 5, no. 4S_Part_6 (2009): P167. http://dx.doi.org/10.1016/j.jalz.2009.05.575.

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Rappoldt, Liam, Adrienne Weeks, Rodney Ouellete, et al. "TMOD-26. ESTABLISHING A PATIENT-DERIVED, IN-VITRO ORGANOTYPIC SLICE CULTURE MODEL OF GBM." Neuro-Oncology 22, Supplement_2 (2020): ii233. http://dx.doi.org/10.1093/neuonc/noaa215.976.

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Abstract Glioblastoma Multiforme (GBM) is the most common primary malignant brain tumour. This tumour is universally fatal with a median survival of 15 months. Driving this pathology is an extremely heterogeneic tumour and complex tumour microenvironment. GBM research is primarily conducted using immortalized or primary cell lines due to their practicality and reproducibility. However, these cell lines do not effectively recapitulate the tumour microenvironment. Mouse models address these shortcomings but are laborious and expensive. We propose to utilize a patient derived organotypic culture

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Laksono, Brigitta M., Diana N. Tran, Ivanela Kondova, et al. "Comparable Infection Level and Tropism of Measles Virus and Canine Distemper Virus in Organotypic Brain Slice Cultures Obtained from Natural Host Species." Viruses 13, no. 8 (2021): 1582. http://dx.doi.org/10.3390/v13081582.

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Measles virus (MV) and canine distemper virus (CDV) are closely related members of the family Paramyxoviridae, genus Morbillivirus. MV infection of humans and non-human primates (NHPs) results in a self-limiting disease, which rarely involves central nervous system (CNS) complications. In contrast, infection of carnivores with CDV usually results in severe disease, in which CNS complications are common and the case-fatality rate is high. To compare the neurovirulence and neurotropism of MV and CDV, we established a short-term organotypic brain slice culture system of the olfactory bulb, hippoc

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Moelgg, Kurt, Faryal Jummun, and Christian Humpel. "Spreading of Beta-Amyloid in Organotypic Mouse Brain Slices and Microglial Elimination and Effects on Cholinergic Neurons." Biomolecules 11, no. 3 (2021): 434. http://dx.doi.org/10.3390/biom11030434.

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The extracellular deposition of β-amyloid (Aβ) is one of the major characteristics in Alzheimer´s disease (AD). The “spreading hypothesis” suggests that a pathological protein (similar to prions) spreads over the entire brain. The aim of the present study was to use organotypic brain slices of postnatal day 8–10 mice. Using collagen hydrogels, we applied different Aβ peptides onto brain slices and analyzed spreading as well as glial reactions after eight weeks of incubation. Our data showed that from all tested Aβ peptides, human Aβ42 had the most potent activity to spread over into adjacent “

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Lange-Asschenfeldt, Christian, Ami P. Raval, Kunjan R. Dave, Daria Mochly-Rosen, Thomas J. Sick, and Miguel A. Pérez-Pinzón. "Epsilon Protein Kinase C Mediated Ischemic Tolerance Requires Activation of the Extracellular Regulated Kinase Pathway in the Organotypic Hippocampal Slice." Journal of Cerebral Blood Flow & Metabolism 24, no. 6 (2004): 636–45. http://dx.doi.org/10.1097/01.wcb.0000121235.42748.bf.

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Ischemic preconditioning (IPC) promotes brain tolerance against subsequent ischemic insults. Using the organotypic hippocampal slice culture, we conducted the present study to investigate (1) the role of adenosine A1 receptor (A1AR) activation in IPC induction, (2) whether epsilon protein kinase C (ɛPKC) activation after IPC is mediated by the phosphoinositol pathway, and (3) whether ɛPKC protection is mediated by the extracellular signal-regulated kinase (ERK) pathway. Our results demonstrate that activation of A1AR emulated IPC, whereas blockade of the A1AR during IPC diminished neuroprotect

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22

Noraberg, J., F. Poulsen, M. Blaabjerg, et al. "Organotypic Hippocampal Slice Cultures for Studies of Brain Damage, Neuroprotection and Neurorepair." Current Drug Target -CNS & Neurological Disorders 4, no. 4 (2005): 435–52. http://dx.doi.org/10.2174/1568007054546108.

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23

Ereifej, Evon S., Mark Ming-Cheng Cheng, Guangzhao Mao, and Pamela J. VandeVord. "Examining the inflammatory response to nanopatterned polydimethylsiloxane using organotypic brain slice methods." Journal of Neuroscience Methods 217, no. 1-2 (2013): 17–25. http://dx.doi.org/10.1016/j.jneumeth.2013.04.023.

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Eugène, Emmanuel, Françoise Cluzeaud, Carmen Cifuentes-Diaz, et al. "An organotypic brain slice preparation from adult patients with temporal lobe epilepsy." Journal of Neuroscience Methods 235 (September 2014): 234–44. http://dx.doi.org/10.1016/j.jneumeth.2014.07.009.

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25

Heine, Claudia, Katja Sygnecka, Nico Scherf, Marcus Grohmann, Annett Bräsigk, and Heike Franke. "P2Y1 receptor mediated neuronal fibre outgrowth in organotypic brain slice co-cultures." Neuropharmacology 93 (June 2015): 252–66. http://dx.doi.org/10.1016/j.neuropharm.2015.02.001.

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26

Jahnsen, Henrik, Bjarne W. Kristensen, Pierre Thiébaud, et al. "Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes." Methods 18, no. 2 (1999): 160–72. http://dx.doi.org/10.1006/meth.1999.0769.

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27

Sidorcenco, Vasile, Luisa Krahnen, Marion Schulz, et al. "Glioblastoma Tissue Slice Tandem-Cultures for Quantitative Evaluation of Inhibitory Effects on Invasion and Growth." Cancers 12, no. 9 (2020): 2707. http://dx.doi.org/10.3390/cancers12092707.

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Glioblastomas (GBMs) are the most malignant brain tumors and are essentially incurable even after extensive surgery, radiotherapy, and chemotherapy, mainly because of extensive infiltration of tumor cells into the adjacent normal tissue. Thus, the evaluation of novel drugs in malignant glioma treatment requires sophisticated ex vivo models that approach the authentic interplay between tumor and host environment while avoiding extensive in vivo studies in animals. This paper describes the standardized setup of an organotypic brain tissue slice tandem-culture system, comprising of normal brain t

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Sullivan, Breandan L., David Leu, Donald M. Taylor, Christian S. Fahlman, and Philip E. Bickler. "Isoflurane Prevents Delayed Cell Death in an Organotypic Slice Culture Model of Cerebral Ischemia." Anesthesiology 96, no. 1 (2002): 189–95. http://dx.doi.org/10.1097/00000542-200201000-00033.

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Background General anesthetics reduce neuronal death caused by focal cerebral ischemia in rodents and by in vitro ischemia in cultured neurons and brain slices. However, in intact animals, the protective effect may enhance neuronal survival for only several days after an ischemic injury, possibly because anesthetics prevent acute but not delayed cell death. To further understand the mechanisms and limitations of volatile anesthetic neuroprotection, the authors developed a rat hippocampal slice culture model of cerebral ischemia that permits assessment of death and survival of neurons for at le

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Marques-Torrejon, Maria Angeles, Ester Gangoso, and Steven M. Pollard. "Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture." Disease Models & Mechanisms 11, no. 2 (2017): dmm031435. http://dx.doi.org/10.1242/dmm.031435.

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Besl, Brigitte, and Peter Fromherz. "Transistor array with an organotypic brain slice: field potential records and synaptic currents." European Journal of Neuroscience 15, no. 6 (2002): 999–1005. http://dx.doi.org/10.1046/j.1460-9568.2002.01943.x.

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Duport, S., F. Robert, D. Muller, G. Grau, L. Parisi, and L. Stoppini. "An in vitro blood-brain barrier model: Cocultures between endothelial cells and organotypic brain slice cultures." Proceedings of the National Academy of Sciences 95, no. 4 (1998): 1840–45. http://dx.doi.org/10.1073/pnas.95.4.1840.

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Förster, Eckart, Shanting Zhao, and Michael Frotscher. "Hyaluronan-associated adhesive cues control fiber segregation in the hippocampus." Development 128, no. 15 (2001): 3029–39. http://dx.doi.org/10.1242/dev.128.15.3029.

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In various brain regions, particularly in the hippocampus, afferent fiber projections terminate in specific layers. Little is known about the molecular cues governing this laminar specificity. To this end we have recently shown that the innervation pattern of entorhinal fibers to the hippocampus is mimicked by the lamina-specific adhesion of entorhinal cells on living hippocampal slices, suggesting a role of adhesion molecules in the positioning of entorhinal fibers. Here, we have analyzed the role of extracellular matrix components in mediating this lamina-specific adhesion. We show that hyal

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Sanders, Robert D., Jing Xu, Yi Shu, et al. "Dexmedetomidine Attenuates Isoflurane-induced Neurocognitive Impairment in Neonatal Rats." Anesthesiology 110, no. 5 (2009): 1077–85. http://dx.doi.org/10.1097/aln.0b013e31819daedd.

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Background Neuroapoptosis is induced by the administration of anesthetic agents to the young. As alpha2 adrenoceptor signaling plays a trophic role during development and is neuroprotective in several settings of neuronal injury, the authors investigated whether dexmedetomidine could provide functional protection against isoflurane-induced injury. Methods Isoflurane-induced injury was provoked in organotypic hippocampal slice cultures in vitro or in vivo in postnatal day 7 rats by a 6-h exposure to 0.75% isoflurane with or without dexmedetomidine. In vivo, the alpha2 adrenoceptor antagonist at

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Morawski, Markus, Alexander Dityatev, Maike Hartlage-Rübsamen, et al. "Tenascin-R promotes assembly of the extracellular matrix of perineuronal nets via clustering of aggrecan." Philosophical Transactions of the Royal Society B: Biological Sciences 369, no. 1654 (2014): 20140046. http://dx.doi.org/10.1098/rstb.2014.0046.

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Perineuronal nets (PNs) in the brains of tenascin-R-deficient ( tn-r −/− ) mice develop in temporal concordance with those of wild-type ( tn-r +/+ ) mice. However, the histological appearance of PNs is abnormal in adult tn-r −/− mice. Here, we investigated whether similar defects are also seen in dissociated and organotypic cultures from hippocampus and forebrain of tn-r −/− mice and whether the structure of PNs could be normalized. In tn-r −/− cultures, accumulations of several extracellular matrix molecules were mostly associated with somata, whereas dendrites were sparsely covered, compared

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Zehendner, Christoph M., Heiko J. Luhmann, and Christoph RW Kuhlmann. "Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model." Journal of Cerebral Blood Flow & Metabolism 29, no. 12 (2009): 1879–84. http://dx.doi.org/10.1038/jcbfm.2009.103.

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The blood–brain barrier (BBB) closely interacts with the neuronal parenchyma in vivo. To replicate this interdependence in vitro, we established a murine coculture model composed of brain endothelial cell (BEC) monolayers with cortical organotypic slice cultures. The morphology of cell types, expression of tight junctions, formation of reactive oxygen species, caspase-3 activity in BECs, and alterations of electrical resistance under physiologic and pathophysiological conditions were investigated. This new BBB model allows the application of techniques such as laser scanning confocal microscop

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Price, Anna L., Russell Holden, James J. H. Park, et al. "RADI-20. Brain metastasis treatment with high energy radiotherapy and Cherenkov radiation-activated phototherapy." Neuro-Oncology Advances 3, Supplement_3 (2021): iii22. http://dx.doi.org/10.1093/noajnl/vdab071.090.

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Abstract Radiation therapy is a mainstay in the treatment of brain metastasis, yet some tumors are resistant, and elsewhere brain recurrence outside the radiation field is common. Phototherapy using UV light-activated compounds can both kill cancer cells directly and trigger an immune response to extend tumor control. Poor penetration depth of ultraviolet light, however, has limited this treatment to superficial tumors. High-energy photon beams create high energy electrons within the patient which in turn produce Cherenkov radiation in the UV spectrum while traveling through tissue. Given that

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Ravi, Vidhya M., Kevin Joseph, Julian Wurm, et al. "Human organotypic brain slice culture: a novel framework for environmental research in neuro-oncology." Life Science Alliance 2, no. 4 (2019): e201900305. http://dx.doi.org/10.26508/lsa.201900305.

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When it comes to the human brain, models that closely mimic in vivo conditions are lacking. Living neuronal tissue is the closest representation of the in vivo human brain outside of a living person. Here, we present a method that can be used to maintain therapeutically resected healthy neuronal tissue for prolonged periods without any discernible changes in tissue vitality, evidenced by immunohistochemistry, genetic expression, and electrophysiology. This method was then used to assess glioblastoma (GBM) progression in its natural environment by microinjection of patient-derived tumor cells i

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Yalcin, Fatih, Alice Buonfiglioli, Ibrahim Efecan Efe, et al. "TMIC-30. MICROGLIA/BRAIN MACROPHAGES PROMOTE GLIOMA GROWTH BY EXPRESSING GLYCOPROTEIN NMB." Neuro-Oncology 21, Supplement_6 (2019): vi254. http://dx.doi.org/10.1093/neuonc/noz175.1064.

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Abstract BACKGROUND Glioma-associated microglia and blood-derived macrophages (GAMs) promote tumor growth in experimental mouse glioma models. Using microarray and RNA sequencing, we have previously shown that GAMs upregulate the expression of Glycoprotein NMB/Osteoactivin (GPNMB) when compared to naïve microglia. GPNMB is a type 1 transmembrane glycoprotein expressed intracellularly under healthy conditions. Malignancies such as glioma induce a translocation into the plasma membrane where the extracellular domain can be cleaved and released. METHODS We used qRT-PCR, immunocytochemistry, Weste

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Kristensen, Bjarne W., Jens Noraberg, Pierre Thiébaud, Milena Koudelka-Hep, and Jens Zimmer. "Biocompatibility of silicon-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures." Brain Research 896, no. 1-2 (2001): 1–17. http://dx.doi.org/10.1016/s0006-8993(00)03304-7.

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McCaughey-Chapman, Amy, and Bronwen Connor. "Rat brain sagittal organotypic slice cultures as an ex vivo dopamine cell loss system." Journal of Neuroscience Methods 277 (February 2017): 83–87. http://dx.doi.org/10.1016/j.jneumeth.2016.12.012.

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Xu, Bin, Sheng-Wen Wu, Chun-Wei Lu, et al. "Oxidative stress involvement in manganese-induced alpha-synuclein oligomerization in organotypic brain slice cultures." Toxicology 305 (March 2013): 71–78. http://dx.doi.org/10.1016/j.tox.2013.01.006.

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Guldimann, Claudia, Beatrice Lejeune, Sandra Hofer, et al. "Ruminant organotypic brain-slice cultures as a model for the investigation of CNS listeriosis." International Journal of Experimental Pathology 93, no. 4 (2012): 259–68. http://dx.doi.org/10.1111/j.1365-2613.2012.00821.x.

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Mewes, Agneta, Heike Franke, and David Singer. "Organotypic Brain Slice Cultures of Adult Transgenic P301S Mice—A Model for Tauopathy Studies." PLoS ONE 7, no. 9 (2012): e45017. http://dx.doi.org/10.1371/journal.pone.0045017.

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Hutzler, Michael, and Peter Fromherz. "Silicon chip with capacitors and transistors for interfacing organotypic brain slice of rat hippocampus." European Journal of Neuroscience 19, no. 8 (2004): 2231–38. http://dx.doi.org/10.1111/j.0953-816x.2004.03311.x.

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Guldimann, C., A. Zurbriggen, B. Lejeune, T. Seuberlich, and A. Oevermann. "Organotypic Brain Slice Cultures as a Tool for the Investigation of Listeriosis in Ruminants." Journal of Comparative Pathology 146, no. 1 (2012): 60. http://dx.doi.org/10.1016/j.jcpa.2011.11.061.

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Jung, Shin, Hyun-Woo Kim, Je-Hyuk Lee, et al. "Brain tumor invasion model system using organotypic brain-slice culture as an alternative to in vivo model." Journal of Cancer Research and Clinical Oncology 128, no. 9 (2002): 469–76. http://dx.doi.org/10.1007/s00432-002-0366-x.

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Renic, Marija, Suresh N. Kumar, Debebe Gebremedhin, et al. "Protective effect of 20-HETE inhibition in a model of oxygen-glucose deprivation in hippocampal slice cultures." American Journal of Physiology-Heart and Circulatory Physiology 302, no. 6 (2012): H1285—H1293. http://dx.doi.org/10.1152/ajpheart.00340.2011.

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Recent studies have indicated that inhibitors of the synthesis of 20-hydroxyeicosatetraenoic acid (20-HETE) may have direct neuroprotective actions since they reduce infarct volume after ischemia reperfusion in the brain without altering blood flow. To explore this possibility, the present study used organotypic hippocampal slice cultures subjected to oxygen-glucose deprivation (OGD) and reoxygenation to examine whether 20-HETE is released by organotypic hippocampal slices after OGD and whether it contributes to neuronal death through the generation of ROS and activation of caspase-3. The prod

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Velasco-Estevez, Maria, Nina Koch, Ilona Klejbor, et al. "EBI2 Is Temporarily Upregulated in MO3.13 Oligodendrocytes during Maturation and Regulates Remyelination in the Organotypic Cerebellar Slice Model." International Journal of Molecular Sciences 22, no. 9 (2021): 4342. http://dx.doi.org/10.3390/ijms22094342.

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The EBI2 receptor regulates the immune system and is expressed in various immune cells including B and T lymphocytes. It is also expressed in astrocytes in the central nervous system (CNS) where it regulates pro-inflammatory cytokine release, cell migration and protects from chemically induced demyelination. Its signaling and expression are implicated in various diseases including multiple sclerosis, where its expression is increased in infiltrating immune cells in the white matter lesions. Here, for the first time, the EBI2 protein in the CNS cells in the human brain was examined. The functio

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Eyüpoglu, Ilker Y., Eric Hahnen, Alexandra Heckel, et al. "Malignant glioma—induced neuronal cell death in an organotypic glioma invasion model." Journal of Neurosurgery 102, no. 4 (2005): 738–44. http://dx.doi.org/10.3171/jns.2005.102.4.0738.

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✓ Rapid growth and diffuse brain infiltration are hallmarks of malignant gliomas. The underlying molecular pathomechanisms of these tumors, however, remain to be determined. The authors present a novel glioma invasion model that allows researchers to monitor consecutively tumor cell proliferation and migration in an organotypic brain environment. Enhanced green fluorescent protein—labeled F98 rat glioma cells were implanted into slice cultures obtained from a rat hippocampus, and tumor growth was microscopically documented up to 20 days in vitro. Invasion along radially oriented migratory stre

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Rakotomamonjy, Jennifer, and Abdel Ghoumari. "Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture." International Journal of Molecular Sciences 20, no. 2 (2019): 285. http://dx.doi.org/10.3390/ijms20020285.

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Endogenous γ-aminobutyric acid (GABA)-dependent activity induces death of developing Purkinje neurons in mouse organotypic cerebellar cultures and the synthetic steroid mifepristone blocks this effect. Here, using brain-derived neurotrophic factor (BDNF) heterozygous mice, we show that BDNF plays no role in immature Purkinje cell death. However, interestingly, BDNF haploinsufficiency impairs neuronal survival induced by mifepristone and GABAA-receptors antagonist (bicuculline) treatments, indicating that the underlying neuroprotective mechanism requires the neurotrophin full expression.

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