Relevant bibliographies by topics / Organotypic cell culture

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Organotypic cell culture'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 February 2022

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Journal articles on the topic "Organotypic cell culture"

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Chitturi Suryaprakash, Ravi Teja, Omar Kujan, Kate Shearston, and Camile S. Farah. "Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review." International Journal of Molecular Sciences 21, no. 24 (2020): 9520. http://dx.doi.org/10.3390/ijms21249520.

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Three-dimensional (3-D) cell culture models, such as spheroids, organoids, and organotypic cultures, are more physiologically representative of the human tumor microenvironment (TME) than traditional two-dimensional (2-D) cell culture models. They have been used as in vitro models to investigate various aspects of oral cancer but, to date, have not be widely used in investigations of the process of oral carcinogenesis. The aim of this scoping review was to evaluate the use of 3-D cell cultures in oral squamous cell carcinoma (OSCC) research, with a particular emphasis on oral carcinogenesis st
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Dufresne, M., R. Warocquier-Clerout, and M. F. Sigot-Luizard. "Cell phenotype characterization in vascular organotypic culture." Journal of Materials Science: Materials in Medicine 5, no. 11 (1994): 824–29. http://dx.doi.org/10.1007/bf00213142.

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Rappoldt, Liam, Adrienne Weeks, Rodney Ouellete, et al. "TMOD-26. ESTABLISHING A PATIENT-DERIVED, IN-VITRO ORGANOTYPIC SLICE CULTURE MODEL OF GBM." Neuro-Oncology 22, Supplement_2 (2020): ii233. http://dx.doi.org/10.1093/neuonc/noaa215.976.

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Abstract Glioblastoma Multiforme (GBM) is the most common primary malignant brain tumour. This tumour is universally fatal with a median survival of 15 months. Driving this pathology is an extremely heterogeneic tumour and complex tumour microenvironment. GBM research is primarily conducted using immortalized or primary cell lines due to their practicality and reproducibility. However, these cell lines do not effectively recapitulate the tumour microenvironment. Mouse models address these shortcomings but are laborious and expensive. We propose to utilize a patient derived organotypic culture
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Kratochwil, K., F. Ekblom, N. Fusenig, G. Cunha, M. Darmon, and R. I. Freshney. "Organ development and organotypic culture." Cytotechnology 2, S3 (1989): 22–26. http://dx.doi.org/10.1007/bf02279719.

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Feigenspan, Andreas, Joachim Bormann, and Heinz Wässle. "Organotypic slice culture of the mammalian retina." Visual Neuroscience 10, no. 2 (1993): 203–17. http://dx.doi.org/10.1017/s0952523800003618.

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AbstractVertical slices of 6-day postnatal (P6) rat retina were cut at a thickness of 100 μm and cultured using the roller-tube technique. After 14–21 days in vitro there was significant distortion of normal retinal architecture, but localized areas of the slices showed the typical pattern of layering of mature retina. The following immunocytochemical markers were used to characterize the different retinal cell types: antibodies against protein kinase C (PKC), calcium binding protein (CabP 28kD), neurofilaments (NF), glia-specific antibodies (GFAP, vimentin), and transmitter-specific antibodie
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Bnjía, Jesús, Michael Sittinger, Philip Pitzke, Eberhard Wilmes, and Claus Hammer. "Synthesis of Human Cartilage Using Organotypic Cell Culture." ORL 55, no. 6 (1993): 347–51. http://dx.doi.org/10.1159/000276453.

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Kloth, Sabine, Elfriede Eckert, Stefan J. Klein, Jan Monzer, Christiane Wanke, and Will W. Minuth. "Gastric epithelium under organotypic perfusion culture." In Vitro Cellular & Developmental Biology - Animal 34, no. 7 (1998): 515–17. http://dx.doi.org/10.1007/s11626-998-0107-9.

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Schuger, L., K. S. O'Shea, B. B. Nelson, and J. Varani. "Organotypic arrangement of mouse embryonic lung cells on a basement membrane extract: involvement of laminin." Development 110, no. 4 (1990): 1091–99. http://dx.doi.org/10.1242/dev.110.4.1091.

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The behavior of embryonic murine lung cells on a basement membrane extract (Matrigel) was investigated. Single cell suspensions generated by trypsinization of lungs removed from day 12 embryos were plated on Matrigel and cultured for up to one week. The basement membrane extract was used as a gel, and as a wet or dried film. In all of these instances, organotypic arrangement of the embryonic lung cells was observed. This process consisted of cell aggregation, sorting, polarization and formation of a tridimensional organization resembling embryonic lung. The maximal degree of organotypic develo
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Sakib, Sadman, Aya Uchida, Paula Valenzuela-Leon, et al. "Formation of organotypic testicular organoids in microwell culture†." Biology of Reproduction 100, no. 6 (2019): 1648–60. http://dx.doi.org/10.1093/biolre/ioz053.

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Abstract Three-dimensional (3D) organoids can serve as an in vitro platform to study cell–cell interactions, tissue development, and toxicology. Development of organoids with tissue architecture similar to testis in vivo has remained a challenge. Here, we present a microwell aggregation approach to establish multicellular 3D testicular organoids from pig, mouse, macaque, and human. The organoids consist of germ cells, Sertoli cells, Leydig cells, and peritubular myoid cells forming a distinct seminiferous epithelium and interstitial compartment separated by a basement membrane. Sertoli cells i
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Pineau, Hailey, and Valerie Sim. "POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research." Biomolecules 10, no. 7 (2020): 1079. http://dx.doi.org/10.3390/biom10071079.

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Prion diseases are fatal, transmissible neurodegenerative disorders whose pathogenesis is driven by the misfolding, self-templating and cell-to-cell spread of the prion protein. Other neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis and Huntington’s disease, share some of these prion-like features, with different aggregation-prone proteins. Consequently, researchers have begun to apply prion-specific techniques, like the prion organotypic slice culture assay (POSCA), to these disorders. In this review we explore the ways in which the pr
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Dissertations / Theses on the topic "Organotypic cell culture"

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Barnhart, Kirstin Faye. "In vitro and in vivo analysis of differential gene expression between normal norfolk terrier dogs and those with an autosomal recessive mutation in KRT10." Texas A&M University, 2004. http://hdl.handle.net/1969.1/2671.

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Natural diseases caused by keratin mutations are rare and have only been reported in humans. We have recently identified a heritable skin disorder in Norfolk terriers caused by a mutation in KRT10. Affected dogs have a tendency to form shallow erosions or blisters following mild trauma, which is first noted after the birthing process. As the dogs age, they display generalized hyperpigmentation and scaling that is most severe in the axillary and inguinal regions. The main histologic and ultrastructural features include: marked hyperkeratosis, epidermal hyperplasia, prominent vacuolation of the
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Arunachalam, Sasi. "The Role of store operated calcium channels in human carcinoid cell lines." University of Toledo Health Science Campus / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=mco1279216983.

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LACROIX, BORGEY BRIGITTE. "Cytodifferenciation de l'intestin humain." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13009.

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Definition du role des facteurs hormonaux, nutritionnels et des interactions epithelio-mesenchymateuses dans la maturation intestinale au cours du developpement et le long de l'axe crypto-villositaire
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Nurmenniemi, S. (Sini). "Analysis of cancer cell invasion with novel in vitro methods based on human tissues." Doctoral thesis, Oulun yliopisto, 2011. http://urn.fi/urn:isbn:9789514295799.

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Abstract Cancer progression is a multistep process dependent on tumour-stroma interactions. Various cell types, such as fibroblasts, endothelial, inflammatory and stem cells, as well as extracellular matrix (ECM) proteins, such as collagens, contribute to the tumour outcome. Tumour growth and invasion is accompanied by the proteolysis of ECM components mediated by various enzymes, such as matrix metalloproteases (MMPs). Proteolytic fragments released into the circulation may reflect cancer progression. The aim of this study was to develop novel in vitro methods for investigating cell invasion
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Steinmeyer, Joseph D. (Joseph Daly). "Rapid single-cell electroporation for labeling organotypic cultures." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/60188.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2010.<br>Vita.<br>Includes bibliographical references (p. 37-39).<br>Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in reasonable periods of time. A novel system that will rapidly load, clean, and accurately position a glass micropipette elect
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N'tumba-Byn, Thierry. "Mécanismes d’action des perturbateurs endocriniens bisphénol A et phtalates sur le développement du testicule fœtal." Thesis, Paris 11, 2013. http://www.theses.fr/2013PA11T006/document.

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Depuis plusieurs années, un nombre conséquent d’études décrivent une augmentation de l’incidence de pathologies liées à la fonction de reproduction masculine. Ces anomalies ont été regroupées sous le terme de « syndrome de dysgénésie testiculaire ». Ce syndrome aurait pour origine les effets délétères de polluants environnementaux sur le développement du testicule en période fœtale. Parmi ces polluants environnementaux, les phtalates et le bisphénol A (BPA) sont les plastifiants les plus produits et les plus répandus dans les objets de consommation courante. De nombreuses études leur sont cons
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Alahuhta, I. (Ilkka). "The microenvironment is essential for OTSCC progression." Doctoral thesis, Oulun yliopisto, 2016. http://urn.fi/urn:isbn:9789526213583.

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Abstract The tumor microenvironment (TME) is critically important for tumor development. The microenvironment consists of fibroblasts, endothelial and immune cells as well as extracellular matrix (ECM), proteases and various other soluble factors produced by the cells. It is challenging to develop methods that appropriately mimic the human microenvironment, but this effort is essential in order to reliably elucidate the properties of potential anti-tumor drugs. The aim of this study was to create new 3D organotypic invasion models based on human tissue that would be used to study the effects o
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Sundquist, E. (Elias). "The role of tumor microenvironment on oral tongue cancer invasion and prognosis." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526217659.

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Abstract Oral tongue squamous cell carcinoma (OTSCC) is the most common cancer of the oral cavity. The 5-year mortality of OTSCC remains at about 50%. The tumor microenvironment (TME) is now recognized as an important factor in cancer progression and metastasis, as well as a tool for prognostication. The aim of this study was to elucidate the roles of TME hypoxia and soluble factors on cancer cell migration and invasion, and the prognostic value of two extracellular matrix (ECM) molecules: tenascin-C (TNC) and fibronectin (FN). Hypoxia was studied using oral squamous cell carcinoma cells in mi
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Kalaani, Joanna. "Molecular guidance of dopaminergic cells transplanted in a mouse model of Parkinson's disease." Thesis, Poitiers, 2016. http://www.theses.fr/2016POIT2252/document.

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La maladie de Parkinson (MP) est caractérisée par une dégénérescence des neurones dopaminergiques de la voie nigrostriée. La thérapie cellulaire, par transplantation intranigrale de cellules fœtales issues de mésencéphale ventral (MV), assure un rétablissement anatomique et fonctionnel de cette voie. Des molécules de guidage axonal (MGA) joueraient ainsi un rôle dans la reconnexion axonale des cellules transplantées. Pour tester cette hypothèse, nous avons étudié l'expression de MGA dans le cerveau adulte intact et dans des cellules destinées à la transplantation, ainsi que dans le cerveau adu
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Väyrynen, O. (Otto). "Factors affecting aggressive oral tongue cancer invasion and development of in vitro models for chemoradiotherapy assay." Doctoral thesis, Oulun yliopisto, 2019. http://urn.fi/urn:isbn:9789526222813.

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Abstract Tumor associated macrophages (TAMs) are linked to the invasion of oral tongue squamous cell carcinoma (OTSCC). We modified THP-1 leukemia cells to M1 (inflammatory), M2 (TAM-like) and R848 (imidazoquinoline-treated) type macrophages in order to examine their interactions with OTSCC-cells (HSC-3) by using different kinds of in vitro migration and invasion models. We observed that interaction of TAM-resembling M2-type macrophages with HSC-3 cells induced invasion and migration, whereas the influence of M1 macrophages reduced them. Patient response to chemoradiotherapy is highly reliant
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Book chapters on the topic "Organotypic cell culture"

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Sundstrom, Lars E., Igor Charvet, and Luc Stoppini. "Organotypic microtissues on an air-liquid interface." In Technology Platforms for 3D Cell Culture. John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781118851647.ch6.

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Ranftl, Romana E., and Fernando Calvo. "Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7021-6_15.

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Kim, Jonghui, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc. "Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model." In Basophils and Mast Cells. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0696-4_8.

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Kim, Jonghui, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc. "Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model." In Basophils and Mast Cells. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1173-8_6.

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Diekmann, S., R. Nitsch, and T. G. Ohm. "The organotypic entorhinal-hippocampal complex slice culture of adolescent rats. A model to study transcellular changes in a circuit particularly vulnerable in neurodegenerative disorders." In Cell and Animal Models in Aging and Dementia Research. Springer Vienna, 1994. http://dx.doi.org/10.1007/978-3-7091-9350-1_5.

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Hofmann, Hans-Dieter, Steffen Schulz-Key, Daniel Hertle, and Matthias Kirsch. "Organotypic Cultures of the Rat Retina." In New Methods for Culturing Cells from Nervous Tissues. KARGER, 2005. http://dx.doi.org/10.1159/000083442.

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Alasia, Silvia, Adalberto Merighi, and Laura Lossi. "Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices." In Neuromethods. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2313-7_18.

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He, Yinghong, and Cristina Has. "Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models." In Skin Tissue Engineering. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9473-1_14.

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Guerrero-Cázares, Hugo, Kaisorn L. Chaichana, and Alfredo Quiñones-Hinojosa. "Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-280-9_6.

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Llano, Isabel, Beat H. Gähwiler, and Alain Marty. "Voltage- and Transmitter-Gated Channels in Purkinje Cells from Organotypic Cerebellar Cultures." In The Cerebellum Revisited. Springer US, 1992. http://dx.doi.org/10.1007/978-1-4612-2840-0_9.

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Conference papers on the topic "Organotypic cell culture"

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Shull, Gabriella, Christiane Haffner, Wieland Huttner, Elena Taverna, and Suhasa B. Kodandaramaiah. "Robotic Platform for the Delivery of Gene Products Into Single Cells in Organotypic Slices of the Developing Mouse Brain." In 2018 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/dmd2018-6899.

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Microinjection of genetic components and dye into organotypic slices provides excellent single cell resolution for unraveling biological complexities, but is extremely difficult and time consuming to perform manually resulting in low yield and low use in the developmental biology field. We developed a computer vision guided platform to inject specimen with mRNA, and/or dye and investigated the efficiency of the process using organotypic slices of the mouse developing neocortex. We demonstrate that the system significantly increases yield of injection relative to manual use by an order of magni
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Engelmann, L., J. Thierauf, HJ Stark, et al. "Introduction of a 3D-organotypic co-culture model for head and neck squamous cell carcinoma." In Abstract- und Posterband – 90. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Digitalisierung in der HNO-Heilkunde. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1685985.

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Toriseva, Mervi, Katja Fagerlund, Jesse Mattsson, et al. "Abstract 1158: Phenotypic screening using AMIDA identifies different drug responses in breast and prostate cancer cell lines in an organotypic cell culture model." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-1158.

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Rhee, Chung-Ku, Young Hoon Kim, Se Hyung Kim, Peijie He, and Jin Chul Ahn. "Effect of low level laser therapy on hair cell regeneration following gentamicin induced ototoxicity in postnatal organotypic culture of rat cochlea." In BiOS, edited by Michael R. Hamblin, Ronald W. Waynant, and Juanita Anders. SPIE, 2010. http://dx.doi.org/10.1117/12.841267.

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Liu, Chao J., Ghaidan Shamsan, Taner Akkin, and David J. Odde. "Multimodality imaging of glioma cells migration in organotypic brain slice culture (Conference Presentation)." In Multimodal Biomedical Imaging XIII, edited by Fred S. Azar and Xavier Intes. SPIE, 2018. http://dx.doi.org/10.1117/12.2289334.

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Goletiani, C., and B. Morrison. "Uric acid prevents traumatic cell death and neuronal dysfunction in organotypic hippocampal slice cultures." In 2010 36th Annual Northeast Bioengineering Conference. IEEE, 2010. http://dx.doi.org/10.1109/nebc.2010.5458109.

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Thierauf, Julia C., Luca Engelmann, Natalia Koerich Laureano, et al. "Abstract 336: Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-336.

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Rossi, Marco, Antonio Tarantino, Renza Roncarati, et al. "Abstract 530: Evaluation ofin vitroinvasion of primary vs recurrent glioblastoma multiforme cells using organotypic rodent brain slice culture." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-530.

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Reports on the topic "Organotypic cell culture"

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Grego, Sonia, Edward R. Dougherty, Francis J. Alexander, et al. Systems Biology for Organotypic Cell Cultures. Office of Scientific and Technical Information (OSTI), 2016. http://dx.doi.org/10.2172/1313549.

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Latimer, Jean J. Identification of Stem Cells in a Novel Human Mammary Epithelial Culture (HMEC) System that Reproducibly Demonstrates Ductal Organotypic Architecture in 3 Weeks. Defense Technical Information Center, 2006. http://dx.doi.org/10.21236/ada458403.

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