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Journal articles on the topic 'Organotypic cell culture'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Chitturi Suryaprakash, Ravi Teja, Omar Kujan, Kate Shearston, and Camile S. Farah. "Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review." International Journal of Molecular Sciences 21, no. 24 (2020): 9520. http://dx.doi.org/10.3390/ijms21249520.

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Three-dimensional (3-D) cell culture models, such as spheroids, organoids, and organotypic cultures, are more physiologically representative of the human tumor microenvironment (TME) than traditional two-dimensional (2-D) cell culture models. They have been used as in vitro models to investigate various aspects of oral cancer but, to date, have not be widely used in investigations of the process of oral carcinogenesis. The aim of this scoping review was to evaluate the use of 3-D cell cultures in oral squamous cell carcinoma (OSCC) research, with a particular emphasis on oral carcinogenesis st

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Dufresne, M., R. Warocquier-Clerout, and M. F. Sigot-Luizard. "Cell phenotype characterization in vascular organotypic culture." Journal of Materials Science: Materials in Medicine 5, no. 11 (1994): 824–29. http://dx.doi.org/10.1007/bf00213142.

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3

Rappoldt, Liam, Adrienne Weeks, Rodney Ouellete, et al. "TMOD-26. ESTABLISHING A PATIENT-DERIVED, IN-VITRO ORGANOTYPIC SLICE CULTURE MODEL OF GBM." Neuro-Oncology 22, Supplement_2 (2020): ii233. http://dx.doi.org/10.1093/neuonc/noaa215.976.

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Abstract Glioblastoma Multiforme (GBM) is the most common primary malignant brain tumour. This tumour is universally fatal with a median survival of 15 months. Driving this pathology is an extremely heterogeneic tumour and complex tumour microenvironment. GBM research is primarily conducted using immortalized or primary cell lines due to their practicality and reproducibility. However, these cell lines do not effectively recapitulate the tumour microenvironment. Mouse models address these shortcomings but are laborious and expensive. We propose to utilize a patient derived organotypic culture

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4

Kratochwil, K., F. Ekblom, N. Fusenig, G. Cunha, M. Darmon, and R. I. Freshney. "Organ development and organotypic culture." Cytotechnology 2, S3 (1989): 22–26. http://dx.doi.org/10.1007/bf02279719.

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Feigenspan, Andreas, Joachim Bormann, and Heinz Wässle. "Organotypic slice culture of the mammalian retina." Visual Neuroscience 10, no. 2 (1993): 203–17. http://dx.doi.org/10.1017/s0952523800003618.

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AbstractVertical slices of 6-day postnatal (P6) rat retina were cut at a thickness of 100 μm and cultured using the roller-tube technique. After 14–21 days in vitro there was significant distortion of normal retinal architecture, but localized areas of the slices showed the typical pattern of layering of mature retina. The following immunocytochemical markers were used to characterize the different retinal cell types: antibodies against protein kinase C (PKC), calcium binding protein (CabP 28kD), neurofilaments (NF), glia-specific antibodies (GFAP, vimentin), and transmitter-specific antibodie

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Bnjía, Jesús, Michael Sittinger, Philip Pitzke, Eberhard Wilmes, and Claus Hammer. "Synthesis of Human Cartilage Using Organotypic Cell Culture." ORL 55, no. 6 (1993): 347–51. http://dx.doi.org/10.1159/000276453.

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7

Kloth, Sabine, Elfriede Eckert, Stefan J. Klein, Jan Monzer, Christiane Wanke, and Will W. Minuth. "Gastric epithelium under organotypic perfusion culture." In Vitro Cellular & Developmental Biology - Animal 34, no. 7 (1998): 515–17. http://dx.doi.org/10.1007/s11626-998-0107-9.

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8

Schuger, L., K. S. O'Shea, B. B. Nelson, and J. Varani. "Organotypic arrangement of mouse embryonic lung cells on a basement membrane extract: involvement of laminin." Development 110, no. 4 (1990): 1091–99. http://dx.doi.org/10.1242/dev.110.4.1091.

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The behavior of embryonic murine lung cells on a basement membrane extract (Matrigel) was investigated. Single cell suspensions generated by trypsinization of lungs removed from day 12 embryos were plated on Matrigel and cultured for up to one week. The basement membrane extract was used as a gel, and as a wet or dried film. In all of these instances, organotypic arrangement of the embryonic lung cells was observed. This process consisted of cell aggregation, sorting, polarization and formation of a tridimensional organization resembling embryonic lung. The maximal degree of organotypic develo

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Sakib, Sadman, Aya Uchida, Paula Valenzuela-Leon, et al. "Formation of organotypic testicular organoids in microwell culture†." Biology of Reproduction 100, no. 6 (2019): 1648–60. http://dx.doi.org/10.1093/biolre/ioz053.

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Abstract Three-dimensional (3D) organoids can serve as an in vitro platform to study cell–cell interactions, tissue development, and toxicology. Development of organoids with tissue architecture similar to testis in vivo has remained a challenge. Here, we present a microwell aggregation approach to establish multicellular 3D testicular organoids from pig, mouse, macaque, and human. The organoids consist of germ cells, Sertoli cells, Leydig cells, and peritubular myoid cells forming a distinct seminiferous epithelium and interstitial compartment separated by a basement membrane. Sertoli cells i

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Pineau, Hailey, and Valerie Sim. "POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research." Biomolecules 10, no. 7 (2020): 1079. http://dx.doi.org/10.3390/biom10071079.

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Prion diseases are fatal, transmissible neurodegenerative disorders whose pathogenesis is driven by the misfolding, self-templating and cell-to-cell spread of the prion protein. Other neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis and Huntington’s disease, share some of these prion-like features, with different aggregation-prone proteins. Consequently, researchers have begun to apply prion-specific techniques, like the prion organotypic slice culture assay (POSCA), to these disorders. In this review we explore the ways in which the pr

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Nagata, Isao, and Norio Nakatsuji. "Migration Behavior of Granule Cell Neurons in Cerebellar Cultures I. A PKH26 Labeling Study in Microexplant and Organotypic Cultures. (mouse cerebellar granule cell/microexplant culture/organotypic explant culture/PKH26/migration)." Development, Growth and Differentiation 36, no. 1 (1994): 19–27. http://dx.doi.org/10.1111/j.1440-169x.1994.00019.x.

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Sypecka, Joanna, Sylwia Koniusz, Maria Kawalec, and Anna Sarnowska. "The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study." Stem Cells International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/471216.

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The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important advantage of the presented model is the preservation of the intrinsic spinal cord fiber tract and the ventrodorsal polarity of the spinal cord. All the processes occurring during axonal growth, regeneration, synapse formation, and myelination could be visualized while being culturedin

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Fennrich, Stefan, Heike Stier, Karl-Josef F�hr, David Ray, Jean-Fran�ois Ghersi-Egea, and Burkhard Schlosshauer. "Organotypic rat brain culture as in vivo-like model system." Methods in Cell Science 18, no. 4 (1996): 283–91. http://dx.doi.org/10.1007/bf00127905.

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Smedowski, Adrian, Marita Pietrucha-Dutczak, Ruchi Maniar, Michael Ajeleti, Iwona Matuszek, and Joanna Lewin-Kowalik. "FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies." Oxidative Medicine and Cellular Longevity 2018 (2018): 1–11. http://dx.doi.org/10.1155/2018/2487473.

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Preclinical toxicity screening of the new retinal compounds is an absolute requirement in the pathway of further drug development. Since retinal neuron cultivation and in vivo studies are relatively expensive and time consuming, we aimed to create a fast and reproducible ex vivo system for retinal toxicity screening. For this purpose, we used rat retinal explant culture that was retrogradely labeled with the FluoroGold before the isolation. Explants were exposed to a toxic concentration of gentamicin and ciliary neurotrophic factor (CNTF), a known neuroprotective agent. The measured outcomes s

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Stachniak, Tevye J. E., and Charles W. Bourque. "Visually guided whole cell patch clamp of mouse supraoptic nucleus neurons in cultured and acute conditions." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 291, no. 1 (2006): R68—R76. http://dx.doi.org/10.1152/ajpregu.00830.2005.

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Recent advances in neuronal culturing techniques have supplied a new set of tools for studying neural tissue, providing effective means to study molecular aspects of regulatory elements in the supraoptic nucleus of the hypothalamus (SON). To combine molecular biology techniques with electrophysiological recording, we modified an organotypic culture protocol to permit transfection and whole cell patch-clamp recordings from SON cells. Neonatal mouse brain coronal sections containing the SON were dissected out, placed on a filter insert in culture medium, and incubated for at least 4 days to allo

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Atterwill, Christopher K., Wendy J. Davies, and Michael A. Kyriakides. "An Investigation of Aluminium Neurotoxicity using some In Vitro Systems." Alternatives to Laboratory Animals 18, no. 1_part_1 (1990): 181–90. http://dx.doi.org/10.1177/026119299001800119.1.

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It has been shown that acute exposure in vitro to high concentrations of aluminium chloride does not appear to perturb neural function in terms of the electrophysiological properties of lower vertebrate leech neurones. Longer term exposure in vitro, however, both non-specifically inhibits cellular differentiation and also produces neural cytotoxicity in the rat midbrain micromass, mixed cell culture model. Furthermore, previous studies from this laboratory have demonstrated a reduction of cholinergic neuronal function in brain organotypic reaggregate cultures following long-term, but not short

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Termini, Lara, and Enrique Boccardo. "Epithelial Organotypic Cultures: A Viable Model to Address Mechanisms of Carcinogenesis by Epitheliotropic Viruses." Current Topics in Medicinal Chemistry 18, no. 4 (2018): 246–55. http://dx.doi.org/10.2174/1568026618666180410125850.

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In vitro culture of primary or established cell lines is one of the leading techniques in many areas of basic biological research. The use of pure or highly enriched cultures of specific cell types obtained from different tissues and genetics backgrounds has greatly contributed to our current understanding of normal and pathological cellular processes. Cells in culture are easily propagated generating an almost endless source of material for experimentation. Besides, they can be manipulated to achieve gene silencing, gene overexpression and genome editing turning possible the dissection of spe

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Ahsan, Aarif, Danny Jeyaraju, Kamlesh Bisht, et al. "Modeling Tumor Microenvironment Interactions of Imid Sensitive and Resistant Cells in 3D Organotypic Culture Models." Blood 132, Supplement 1 (2018): 5622. http://dx.doi.org/10.1182/blood-2018-99-118885.

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Abstract Background: Organotypic culture models developed using 3D conditions recapitulate tissue-specific structural features and cell-cell interactions more accurately than conventional 2D cultures. Our ultimate goal is to optimize culture conditions which promote the survival and proliferation of multiple myeloma (MM) cells and could serve as a platform for molecular mechanistic, clinical biomarker and pharmacodynamic marker studies using immune-modulatory compounds (IMIDs) and other myeloma drugs alone and in combination. Design/Results: Using gas permeable microfluidic devices, we culture

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Dey, Nandini, Yuliang Sun, Amy K. Krie, et al. "Three dimensional organotypic ex vivo culture of tissues from post-operated tumor samples: Strengths and limitations." Journal of Clinical Oncology 35, no. 15_suppl (2017): e23151-e23151. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.e23151.

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e23151 Background: Evolution of tumor occurs in two phases, pretreatment phase, and posttreatment phase.Tumorigenic history and path of tumorigenic evolution determine the response of tumor cells to antitumor drug and thus the outcome of treatment. Carcinomas from the same organ-site with similar genomic alterations in different patients may vary in their tumorigenic history and their diverse paths of tumorigenic evolution which cause them respond differently to the same antitumor drugs. Uniqueness of tumorigenic history and paths of tumorigenic evolution in individual patient makes it ideal t

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Paoli, Carlotta, and Alessandro Carrer. "Organotypic Culture of Acinar Cells for the Study of Pancreatic Cancer Initiation." Cancers 12, no. 9 (2020): 2606. http://dx.doi.org/10.3390/cancers12092606.

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The carcinogenesis of pancreatic ductal adenocarcinoma (PDA) progresses according to multi-step evolution, whereby the disease acquires increasingly aggressive pathological features. On the other hand, disease inception is poorly investigated. Decoding the cascade of events that leads to oncogenic transformation is crucial to design strategies for early diagnosis as well as to tackle tumor onset. Lineage-tracing experiments demonstrated that pancreatic cancerous lesions originate from acinar cells, a highly specialized cell type in the pancreatic epithelium. Primary acinar cells can survive in

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Biggs, Thelma, Janet Foreman, Lars Sundstrom, Urs Regenass, and Francois Lehembre. "Antitumor Compound Testing in Glioblastoma Organotypic Brain Cultures." Journal of Biomolecular Screening 16, no. 8 (2011): 805–17. http://dx.doi.org/10.1177/1087057111414895.

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Glioblastoma multiforme (GBM) is the most common and most aggressive type of primary brain tumor. Identification of new therapeutic regimens is urgently needed. A major challenge remains the development of a relevant in vitro model system with the necessary capacity and flexibility to profile compounds. The authors have developed and characterized a 3D culture system of brain cells (brain Hi-Spot) where GBM-derived cells can be incorporated (GBM/brain Hi-Spot). Immuno-fluorescence and electrophysiological recordings demonstrate that brain Hi-Spots recapitulate many features of brain tissue. Wi

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GIANINAZZI, C., M. SCHILD, N. MÜLLER, et al. "Organotypic slice cultures from rat brain tissue: a new approach forNaegleria fowleriCNS infectionin vitro." Parasitology 132, no. 6 (2005): 797–804. http://dx.doi.org/10.1017/s0031182005008619.

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The free-living amoebaNaegleria fowleriis the aetiological agent of primary amoebic meningoencephalitis (PAM), a disease leading to death in the vast majority of cases. In patients suffering from PAM, and in corresponding animal models, the brain undergoes a massive inflammatory response, followed by haemorrhage and severe tissue necrosis. Both,in vivoandin vitromodels are currently being used to study PAM infection. However, animal models may pose ethical issues, are dependent upon availability of specific infrastructural facilities, and are time-consuming and costly. Conversely, cell culture

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Aydin, Malik, Ella A. Naumova, Aliyah Bellm, et al. "From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection." Viruses 13, no. 3 (2021): 387. http://dx.doi.org/10.3390/v13030387.

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Understanding the response to viral infection in the context of respiratory diseases is of significant importance. Recently, there has been more focus on the role of the nasal epithelium in disease modeling. Here, we provide an overview of different submerged, organotypic 3D and spheroid cell culture models of nasal epithelial cells, which were used to study asthma and allergy with a special focus on virus infection. In detail, this review summarizes the importance, benefits, and disadvantages of patient-derived cell culture models of nasal- and bronchial epithelial cells, including a comparis

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Sullivan, Breandan L., David Leu, Donald M. Taylor, Christian S. Fahlman, and Philip E. Bickler. "Isoflurane Prevents Delayed Cell Death in an Organotypic Slice Culture Model of Cerebral Ischemia." Anesthesiology 96, no. 1 (2002): 189–95. http://dx.doi.org/10.1097/00000542-200201000-00033.

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Background General anesthetics reduce neuronal death caused by focal cerebral ischemia in rodents and by in vitro ischemia in cultured neurons and brain slices. However, in intact animals, the protective effect may enhance neuronal survival for only several days after an ischemic injury, possibly because anesthetics prevent acute but not delayed cell death. To further understand the mechanisms and limitations of volatile anesthetic neuroprotection, the authors developed a rat hippocampal slice culture model of cerebral ischemia that permits assessment of death and survival of neurons for at le

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Lebrun, C., H. X. Avci, R. Wehrlé, et al. "Klf9 is necessary and sufficient for Purkinje cell survival in organotypic culture." Molecular and Cellular Neuroscience 54 (May 2013): 9–21. http://dx.doi.org/10.1016/j.mcn.2012.11.010.

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Garle, Michael J., and Jeffrey R. Fry. "A Comparison of Hepatic Enzyme Activities and their Modulation by Dexamethazone in Freshly Isolated and Cultured Hepatocytes and in the Differentiated Hepatoma Cell Line, 2sFou." Alternatives to Laboratory Animals 24, no. 1 (1996): 31–37. http://dx.doi.org/10.1177/026119299602400106.

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Rodent hepatocytes are mitotically inhibited and lose hepatospecific functions over time in culture. In contrast, some differentiated hepatoma cell lines express stable hepatospecific functions in culture, but at much lower levels than those initially found in primary hepatocytes. A number of hepatospecific functions were measured in freshly isolated and cultured rat hepatocytes; these were compared to activities found in the differentiated Reuber hepatoma cell line, 2sFou. The effects of dexamethazone on these activities were also investigated, since dexamethazone is reported to enhance the e

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Toda, Shuji, Shigehisa Aoki, Kazuyoshi Uchihashi, et al. "Culture Models for Studying Thyroid Biology and Disorders." ISRN Endocrinology 2011 (July 12, 2011): 1–9. http://dx.doi.org/10.5402/2011/275782.

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The thyroid is composed of thyroid follicles supported by extracellular matrix, capillary network, and stromal cell types such as fibroblasts. The follicles consist of thyrocytes and C cells. In this microenvironment, thyrocytes are highly integrated in their specific structural and functional polarization, but monolayer and floating cultures cannot allow thyrocytes to organize the follicles with such polarity. In contrast, three-dimensional (3-D) collagen gel culture enables thyrocytes to form 3-D follicles with normal polarity. However, these systems never reconstruct the follicles consistin

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Mandys, Václav, Katerina Jirsová, and Jirí Vrana. "Neurotoxicity of Seven MEIC Chemicals Evaluated in Organotypic Cultures of Chick Embryonic Dorsal Root Ganglia." Alternatives to Laboratory Animals 25, no. 3 (1997): 303–9. http://dx.doi.org/10.1177/026119299702500311.

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The neurotoxic effects of seven selected Multicenter Evaluation of In Vitro Cytotoxicity programme chemicals (methanol, ethanol, isopropanol, sodium chloride, potassium chloride, iron [II] sulphate and chloroform) were evaluated in organotypic cultures of chick embryonic dorsal root ganglia (DRG), maintained in a soft agar culture medium. Two growth parameters of neurite outgrowth from the ganglia — the mean radial length of neurites and the area of neurite outgrowth — were used to evaluate the toxicities of the chemicals. Dose-dependent decreases of both parameters were observed in all experi

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POOLE, I. CAROLINE LE, RENE M. J. G. J. WIJNGAARD, WIETE WESTERHOF, et al. "Organotypic Culture of Human Skin to Study Melanocyte Migration." Pigment Cell Research 7, no. 1 (1994): 33–43. http://dx.doi.org/10.1111/j.1600-0749.1994.tb00016.x.

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Biancotti, Juan C., Kendal A. Walker, Guihua Jiang, Julie Di Bernardo, Lonnie D. Shea, and Shaun M. Kunisaki. "Hydrogel and neural progenitor cell delivery supports organotypic fetal spinal cord development in an ex vivo model of prenatal spina bifida repair." Journal of Tissue Engineering 11 (January 2020): 204173142094383. http://dx.doi.org/10.1177/2041731420943833.

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Studying how the fetal spinal cord regenerates in an ex vivo model of spina bifida repair may provide insights into the development of new tissue engineering treatment strategies to better optimize neurologic function in affected patients. Here, we developed hydrogel surgical patches designed for prenatal repair of myelomeningocele defects and demonstrated viability of both human and rat neural progenitor donor cells within this three-dimensional scaffold microenvironment. We then established an organotypic slice culture model using transverse lumbar spinal cord slices harvested from retinoic

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Liu, Jinghua, Nancy M. Walker, Matthew T. Cook, Akifumi Ootani, and Lane L. Clarke. "Functional Cftr in crypt epithelium of organotypic enteroid cultures from murine small intestine." American Journal of Physiology-Cell Physiology 302, no. 10 (2012): C1492—C1503. http://dx.doi.org/10.1152/ajpcell.00392.2011.

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Physiological studies of intact crypt epithelium have been limited by problems of accessibility in vivo and dedifferentiation in standard primary culture. Investigations of murine intestinal stem cells have recently yielded a primary intestinal culture in three-dimensional gel suspension that recapitulates crypt structure and epithelial differentiation (Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, Van Es JH, Abo A, Kujala P, Peters PJ, Clevers H. Nature 459: 262–265, 2009). We investigated the utility of murine intestinal crypt cultures (termed “enteroids”) for physio

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Husseman, Jacob W., Kwang Pak, Eduardo Chavez, and Allen Ryan. "R443 – Organotypic Co-Culture of Spiral Ganglion and Organ of Corti." Otolaryngology–Head and Neck Surgery 139, no. 2_suppl (2008): P192—P193. http://dx.doi.org/10.1016/j.otohns.2008.05.599.

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Problem The ability to carry out in vitro culture of the auditory neuroepithelium has provided a powerful means of studying inner ear development. Recently, we have developed an organotypic culture technique that mimics the perinatal cochlea in vivo. Methods Using sterile microdissection and in vitro methods, we have been able to co-culture explanted spiral ganglion (SG) with separate explanted organ of Corti (oC) from different neonatal mice. The SG and oC were co-cultured in their correct anatomical positions. Success of the technique appears dependent on the use of culture plate inserts whi

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Harada, Yoshinori, Masaki Iwai, Takahiro Mori, et al. "Long-Term Organotypic Slice Culture of the Neonatal Mouse Liver." Acta Histochemica et Cytochemica 30, no. 4 (1997): 395–99. http://dx.doi.org/10.1267/ahc.30.395.

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Ito, S., K. Ishimori, and S. Ishikawa. "One-month repeated cigarette smoke exposure of human organotypic bronchial epithelial cell culture." Toxicology Letters 295 (October 2018): S69. http://dx.doi.org/10.1016/j.toxlet.2018.06.519.

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Shin, Hyun-Soo, Hye Jin Hong, Won-Gun Koh, and Jae-Yol Lim. "Organotypic 3D Culture in Nanoscaffold Microwells Supports Salivary Gland Stem-Cell-Based Organization." ACS Biomaterials Science & Engineering 4, no. 12 (2018): 4311–20. http://dx.doi.org/10.1021/acsbiomaterials.8b00894.

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Marques-Torrejon, Maria Angeles, Ester Gangoso, and Steven M. Pollard. "Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture." Disease Models & Mechanisms 11, no. 2 (2017): dmm031435. http://dx.doi.org/10.1242/dmm.031435.

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Nyström, ML, GJ Thomas, M. Stone, IC Mackenzie, IR Hart, and JF Marshall. "Development of a quantitative method to analyse tumour cell invasion in organotypic culture." Journal of Pathology 205, no. 4 (2005): 468–75. http://dx.doi.org/10.1002/path.1716.

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Kuntsche, Judith, Heike Bunjes, Alfred Fahr, et al. "Interaction of lipid nanoparticles with human epidermis and an organotypic cell culture model." International Journal of Pharmaceutics 354, no. 1-2 (2008): 180–95. http://dx.doi.org/10.1016/j.ijpharm.2007.08.028.

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Ivanova, Polina Nicolaevna, Ekaterina Sergeevna Zalomaeva, Sergei Viktorovich Surma, et al. "Impact of weakened geomagnetic field on the organotypic cell culture of various genesis." Molekulyarnaya Meditsina (Molecular medicine) 19, no. 4 (2021): 47–51. http://dx.doi.org/10.29296/24999490-2021-04-08.

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Larsen, Trine R., Ann-Sofi Söderling, Kenneth Caidahl, Peter Roepstorff, and Jan Bert Gramsbergen. "Nitration of soluble proteins in organotypic culture models of Parkinson's disease." Neurochemistry International 52, no. 3 (2008): 487–94. http://dx.doi.org/10.1016/j.neuint.2007.08.008.

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Iordachescu, Alexandra, Richard L. Williams, Philippa A. Hulley, and Liam M. Grover. "Organotypic Culture of Bone-Like Structures Using Composite Ceramic-Fibrin Scaffolds." Current Protocols in Stem Cell Biology 48, no. 1 (2019): e79. http://dx.doi.org/10.1002/cpsc.79.

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Klausner, Mitchell, Yuki Handa, and Seiya Aizawa. "In vitro three-dimensional organotypic culture models of the oral mucosa." In Vitro Cellular & Developmental Biology - Animal 57, no. 2 (2021): 148–59. http://dx.doi.org/10.1007/s11626-020-00539-1.

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Smola, Hans, Hans-Jürgen Stark, Gabi Thiekötter, Nicolae Mirancea, Thomas Krieg, and Norbert E. Fusenig. "Dynamics of Basement Membrane Formation by Keratinocyte–Fibroblast Interactions in Organotypic Skin Culture." Experimental Cell Research 239, no. 2 (1998): 399–410. http://dx.doi.org/10.1006/excr.1997.3910.

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Meyers, Craig. "Organotypic (raft) epithelial tissue culture system for the differentiation-dependent replication of papillomavirus." Methods in Cell Science 18, no. 3 (1996): 201–10. http://dx.doi.org/10.1007/bf00132885.

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Pineau, Hailey, and Valerie L. Sim. "From Cell Culture to Organoids-Model Systems for Investigating Prion Strain Characteristics." Biomolecules 11, no. 1 (2021): 106. http://dx.doi.org/10.3390/biom11010106.

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Prion diseases are the hallmark protein folding neurodegenerative disease. Their transmissible nature has allowed for the development of many different cellular models of disease where prion propagation and sometimes pathology can be induced. This review examines the range of simple cell cultures to more complex neurospheres, organoid, and organotypic slice cultures that have been used to study prion disease pathogenesis and to test therapeutics. We highlight the advantages and disadvantages of each system, giving special consideration to the importance of strains when choosing a model and whe

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Canning, B. J., B. J. Undem, P. C. Karakousis, and R. D. Dey. "Effects of organotypic culture on parasympathetic innervation of guinea pig trachealis." American Journal of Physiology-Lung Cellular and Molecular Physiology 271, no. 5 (1996): L698—L706. http://dx.doi.org/10.1152/ajplung.1996.271.5.l698.

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Nonadrenergic, noncholinergic (NANC) relaxations of airway smooth muscle are thought to be mediated by vasoactive intestinal peptide (VIP) and nitric oxide (NO). Previous studies of the parasympathetic innervation of guinea pig trachealis suggest that the ganglion neurons mediating NANC relaxations but not cholinergic contractions are associated with the esophagus. In this study, the location of the neurons mediating these responses and their neurochemical phenotype was further assessed. Guinea pig tracheas maintained in organotypic culture for 2 days with the adjacent esophagus intact display

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Hedberg, Jesper J., Annette Hansson, Jan A. Nilsson, Jan-Olov Höög, and Roland C. Grafström. "Uniform Expression of Alcohol Dehydrogenase 3 in Epithelia Regenerated with Cultured Normal, Immortalised and Malignant Human Oral Keratinocytes." Alternatives to Laboratory Animals 29, no. 3 (2001): 325–33. http://dx.doi.org/10.1177/026119290102900308.

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The human oral epithelium is a target for damage from the inhalation of formaldehyde. However, most experimental studies on this chemical have relied on laboratory animals that are obligatory nose breathers, including rats and mice. Therefore, in vitro model systems that mimic the structure of the human oral epithelium and which retain normal tissue-specific metabolic competence are desirable. Based on the established role of alcohol dehydrogenase 3 (ADH3), also known as glutathione-dependent formaldehyde dehydrogenase, as the primary enzyme catalysing the detoxification of formaldehyde, the a

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Phillips, Wiktor S., Mikkel Herly, Christopher A. Del Negro, and Jens C. Rekling. "Organotypic slice cultures containing the preBötzinger complex generate respiratory-like rhythms." Journal of Neurophysiology 115, no. 2 (2016): 1063–70. http://dx.doi.org/10.1152/jn.00904.2015.

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Study of acute brain stem slice preparations in vitro has advanced our understanding of the cellular and synaptic mechanisms of respiratory rhythm generation, but their inherent limitations preclude long-term manipulation and recording experiments. In the current study, we have developed an organotypic slice culture preparation containing the preBötzinger complex (preBötC), the core inspiratory rhythm generator of the ventrolateral brain stem. We measured bilateral synchronous network oscillations, using calcium-sensitive fluorescent dyes, in both ventrolateral (presumably the preBötC) and dor

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Eyüpoglu, Ilker Y., Eric Hahnen, Alexandra Heckel, et al. "Malignant glioma—induced neuronal cell death in an organotypic glioma invasion model." Journal of Neurosurgery 102, no. 4 (2005): 738–44. http://dx.doi.org/10.3171/jns.2005.102.4.0738.

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✓ Rapid growth and diffuse brain infiltration are hallmarks of malignant gliomas. The underlying molecular pathomechanisms of these tumors, however, remain to be determined. The authors present a novel glioma invasion model that allows researchers to monitor consecutively tumor cell proliferation and migration in an organotypic brain environment. Enhanced green fluorescent protein—labeled F98 rat glioma cells were implanted into slice cultures obtained from a rat hippocampus, and tumor growth was microscopically documented up to 20 days in vitro. Invasion along radially oriented migratory stre

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Mateu-Sanz, Miguel, Juan Tornín, Bénédicte Brulin, et al. "Cold Plasma-Treated Ringer’s Saline: A Weapon to Target Osteosarcoma." Cancers 12, no. 1 (2020): 227. http://dx.doi.org/10.3390/cancers12010227.

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Osteosarcoma (OS) is the main primary bone cancer, presenting poor prognosis and difficult treatment. An innovative therapy may be found in cold plasmas, which show anti-cancer effects related to the generation of reactive oxygen and nitrogen species in liquids. In vitro models are based on the effects of plasma-treated culture media on cell cultures. However, effects of plasma-activated saline solutions with clinical application have not yet been explored in OS. The aim of this study is to obtain mechanistic insights on the action of plasma-activated Ringer’s saline (PAR) for OS therapy in ce

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