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1
Efimova, Natalia Nikolaevna. "Sound Editing in Screen Works." Journal of Flm Arts and Film Studies 7, no. 2 (June 15, 2015): 73–81. http://dx.doi.org/10.17816/vgik7273-81.
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The article pinpoints peculiarities of sound editing in movies basing on analysis of partitions of popular films of40-90s; the most frequent principles of sound track arrangement are examined for the first time. The stuff selection is conditioned by measure of popularity of screen works in question. Due to talent of such famous composers as I. Dunaevsky, S. Prokofiev, A. Khachaturian, A. Pakhmutova, A. Petrov et al and their ability to hear plastic imagery, to comprehend filmic atmosphere music plays an extremely important part in these films. Many songs from these films are still in circulation even now. Thorough sound design and editing are of great significance in film production. The author comes to conclusion, that rondo as a musical form and leit-motif as a principle of musical stuff development form a dominant principle of sound stuff arrangement. The two fundamentally tighten the structure of the film. Since original music affords to accentuate sound effects in the most adequate way, it seems perfect to call to a composer for creating original music. The author assumes, that the choice of sound arrangement principle in cinema depends on deliberate conception of the film, wrought out by the helmer, composer, and supervising sound editor. The screen works property is closely bound with attentive partition editing.
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Anderson, Laura. "Sonic ‘Detheatricalization’: Jean Cocteau, Film Music, and ‘Les Parents Terribles’." Music and Letters 100, no. 4 (November 1, 2019): 654–84. http://dx.doi.org/10.1093/ml/gcz081.
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Abstract Jean Cocteau’s adaptation of his controversial play Les Parents terribles for the screen stands out in his oeuvre as an attempt to reconcile theatre and cinema. It also presented a challenge in preparing a soundscape for a work that did not have any music in its original form. Parents occupies a unique, Janus-like position in the history of French film music, as forward-looking in its anticipation of New Wave treatment of music as material as it is representative of the turn to adapting stage plays for the screen that started in the 1930s. Drawing on production sketchbooks and testimonies, this article considers the development of Cocteau’s working method and his collaboration with Georges Auric, fuelled by the director’s desire to take control of sonic matters. The resulting employment of a monothematic score was not only a new solution to the famous problem of filmed theatre, ‘detheatricalizing’ Parents sonically and visually, it contributed considerably to the development of Cocteau’s status as film auteur—one whose role now extended to adapting musical material. Furthermore, the effect of this compositional technique in Parents suggests that it can be fruitfully situated in relation to recent work in film music studies on issues of anempathetic scoring practices.
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El Maï, Mounir, Serena Janho dit Hreich, Cedric Gaggioli, Armelle Roisin, Nicole Wagner, Jing Ye, Pierre Jalinot, Julien Cherfils-Vicini, and Eric Gilson. "A Novel Screen for Expression Regulators of the Telomeric Protein TRF2 Identified Small Molecules That Impair TRF2 Dependent Immunosuppression and Tumor Growth." Cancers 13, no. 12 (June 15, 2021): 2998. http://dx.doi.org/10.3390/cancers13122998.
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Telomeric repeat-binding factor 2 (TRF2) is a subunit of the shelterin protein complex, which binds to and protects telomeres from unwanted DNA damage response (DDR) activation. TRF2 expression plays a pivotal role in aging and cancer, being downregulated during cellular senescence and overexpressed during oncogenesis. Cancers overexpressing TRF2 often exhibit a poor prognosis. In cancer cells, TRF2 plays multiple functions, including telomere protection and non-cell autonomous roles, promoting neo-angiogenesis and immunosuppression. We present here an original screening strategy, which enables identification of small molecules that decrease or increase TRF2 expression. By screening a small library of Food and Drug Agency (FDA)-approved drugs, we identified two molecules (AR-A014418 and alexidine·2HCl) that impaired tumor growth, neo-angiogenesis and immunosuppression by downregulating TRF2 expression in a mouse xenograft model. These results support the chemotherapeutic strategy of downregulating TRF2 expression to treat aggressive human tumors and validate this cell-based assay capable of screening for potential anti-cancer and anti-aging molecules by modulating TRF2 expression levels.
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Del Priore, V., C. Heath, C. Snay, A. MacMillan, L. Gorsch, S. Dagher, and C. Cole. "A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae." Journal of Cell Science 110, no. 23 (December 1, 1997): 2987–99. http://dx.doi.org/10.1242/jcs.110.23.2987.
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Rat7p/Nup159p is an essential nucleoporin of Sac-charomyces cerevisiae originally isolated in a genetic screen designed to identify yeast temperature-sensitive mutants defective in mRNA export. Here we describe a detailed structural-functional analysis of Rat7p/Nup159p. The mutation in the rat7-1 ts allele, isolated in the original genetic screen, was found to be a single base pair change that created a stop codon approximately 100 amino acids upstream of the actual stop codon of this 1,460 amino acid polypeptide, thus eliminating one of the two predicted coiled-coil regions located near the carboxyl terminus of the protein. These coiled-coil regions are essential since an allele lacking both coiled-coil regions was unable to support growth under any conditions. In contrast, no other region of the protein was absolutely required. The SAFG/PSFG repeat region in the central third of the protein was completely dispensable for growth at temperatures between 16 degrees C and 37 degrees C and cells expressing this mutant allele were indistinguishable from wild type. Deletion of the amino-terminal third of the protein, upstream from the repeat region, or the portion between the repeat region and the coiled-coils resulted in temperature-sensitivity, but the two alleles showed distinct phenotypes with respect to the behavior of nuclear pore complexes (NPCs). Taken together, our data suggest that Rat7p/Nup159p is anchored within the NPC through its coiled-coil region and adjacent sequences. In addition, we postulate that the N-terminal third of Rat7p/Nup159p plays an important role in mRNA export.
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Kruger, Jan-Louis. "Ideology and Subtitling: South African Soap Operas." Broadcasting with Intent 57, no. 2 (February 4, 2013): 496–509. http://dx.doi.org/10.7202/1013958ar.
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This article investigates the ideological component of patronage in the subtitling of four South African soap operas: Generations, 7de Laan, Muvhango, and Isidingo. Taking the concepts introduced by Lefevere as point of departure, the article first discusses the various ways in which audiovisual translation (AVT) is subject to manipulation. This manipulation is shown to be a result of the fact that subtitles, as text superimposed onto the image during post-editing, thereby obscuring a small part of the screen, constantly foregrounds itself to the audience. This foregrounding is also affected by the linguistic background of the audience – whether or not they understand the original dialogue. The argument then turns to a discussion of AVT, and specifically subtitling, as rewriting. The link between language and ideology is discussed as it pertains to issues of power, particularly related to the role of English in the media, also in South Africa, where, in Gottlieb’s terminology, South Africa can be described as a multilingual anglophile context. The language policy of the South African Broadcasting Corporation is then discussed in terms of patronage and ideology followed by a discussion of the role of ideology in these four locally-produced soap operas. In this discussion the different ways in which the subtitling practices of the soap operas reflect ideology are investigated. The article concludes that accessibility plays a smaller role in subtitling in South Africa than the ideology of multilingualism and multiculturalism.
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Gozdecka, Malgorzata, Monika Dudek, Konstantinos Tzelepis, Aristi Damaskou, Vijay Baskar, Penny Wright, Graham Duddy, et al. "Genetic Vulnerabilities of DNMT3AR882H in Myeloid Malignancies." Blood 134, Supplement_1 (November 13, 2019): 111. http://dx.doi.org/10.1182/blood-2019-126505.
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Mutations affecting the gene for the de novo DNA Methyltransferase 3A (DNMT3A) are the most common drivers of clonal hematopoiesis (CH) and amongst the most common somatic events in acute myeloid leukaemia (AML). Approximately two thirds of AML-associated DNMT3A mutations are heterozygous substitutions affecting codon R882, located within the methytransferase domain and are correlated with global hypomethylation. DNMT3A mutations are initiating events in leukemogenesis and leukemic progression relies on the acquisition of additional mutations in genes such as NPM1, TET2, IDH1/2, FLT3 andNRAS. AMLs harboring DNMT3A-R882 mutations display increased resistance to chemotherapy and carry a worse prognosis for patients, but the molecular basis of this is not well understood. To improve our understanding of the molecular effects of mutant DNMT3A, we developed a conditional model of DNMT3A-R882H. Our conditional Dnmt3a-flox-R882H allele retains the native locus intact, but generates the Dnmt3a-R882H mutant upon Cre-loxP recombination (Mx1-Cre). We observed that Dnmt3aR882H/+ mutant bone marrow (BM) cells had markedly enhanced self-renewal potential in serial re-plating assays and increased BM repopulation ability in competitive transplants. Approximately 30% of Dnmt3aR882H/+ mice developed leukaemia after a long latency (median of 532 days). Introduction of Flt3-ITD mutation significantly accelerated spontaneous disease, in agreement with other published reports, with majority of mice succumbing to AML after a median of 192 days. Due to the frequent co-occurrence and strong cooperation between Dnmt3aR882H/+ with Flt3ITD/+, we sought to identify potential genetic vulnerabilities of this combination, to facilitate patient treatment. We therefore generated Dnmt3aR882H/+/Flt3ITD/+/Mx1-Cre+/Rosa-Cas9+ mice, induced Dnmt3aR882H mutation with pIpC and isolated hematopoietic stem and progenitor cells (HSPCs). We cultured HSPCs in vitro and performed whole-genome CRISPR dropout screens to identify genetic vulnerabilities. This identified 526 dropout genes at a genome-wide FDR of 20%, including pan-essential genes such as components of the ribosome, proteasome or spliceosome. To identify essential genes specific to the Dnmt3aR882H/+/Flt3ITD/+ genotype, we performed analogous screens in the non-leukemic mouse hematopoietic precursor cell-7 (HPC-7) and used published data indicating essential genes for normal human CD34 cells. Overlap of these screens revealed 196 dropouts present only in Dnmt3aR882H/+/Flt3ITD/+/Mx1-Cre+/Rosa-Cas9+ cells. Among these were genes coding for "druggable" candidates such as kinases and histone modifying enzymes, including SET Domain Containing 1B (Setd1b), one of the six mammalian proteins (also Setd1a and Mll1-4) that can catalyze methylation of H3K4. Each of these six proteins can interact with obligate proteins as a part of the methyltransferase complex, but also form distinct complexes with non-redundant function. We firstly validated that the knockout of Setd1b induced by gRNA significantly reduced the growth of pre-leukemic and leukemic HSPCs from both Dnmt3aR882H/+/Flt3ITD/+/Mx1-Cre+/Rosa-Cas9+ and Dnmt3aR882H/+/Mx1-Cre+/Rosa-Cas9+ mice. Importantly, Setd1b deletion in normal HSPC had no major effect on cell growth. To investigate the molecular role of Setd1b in Dnmt3aR882H context, we asked if Setd1b catalytic activity and linked transcriptional programs were responsible for the observed phenotypes. For this, we performed histone profiling and RNA-sequencing in Dnmt3aR882H/+/Flt3ITD/+/Mx1-Cre+/Rosa-Cas9+HSPC upon Setd1b KO vs control and observed a striking reduction of H3K4me3 marks at particular loci in association with loss of expression of corresponding genes. These included several known to be required for cellular proliferation and transformation, some of which were themselves dropouts in our original screen. Importantly the majority of genes were also significantly upregulated when gene expression from Dnmt3aR882H/+/Flt3ITD/+ HSPC were compared with Flt3ITD/+ HSPC. Our data propose that the catalytic activity of Setd1b plays a mechanistic role in driving the growth/fitness-promoting effects of Dnmt3aR882H/+. Blocking the catalytic activity of SETD1B may therefore represent a plausible therapeutic option for the treatment of AML of even CH patients harboring mutations inDNMT3A. Disclosures Mazan: Selvita S.A.: Employment. Vassiliou:Kymab Ltd: Consultancy, Other: Minor Stockholder; Oxstem Ltd: Consultancy; Celgene: Research Funding.
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Gutierrez, Alejandro, Hui Feng, Prochownik Edward, John Kanki, and A. Thomas Look. "A Tamoxifen-Dependent Conditional Model of MYC-Induced T Cell Acute Lymphoblastic Leukemia in the Zebrafish." Blood 110, no. 11 (November 16, 2007): 2808. http://dx.doi.org/10.1182/blood.v110.11.2808.2808.
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Abstract The MYC oncogene plays a central role in the pathogenesis of human T cell acute lymphoblastic leukemia (T-ALL), and our laboratory has previously developed a zebrafish model of Myc-induced T-ALL. The primary strength of the zebrafish as a model system for human disease lies in is its suitability for unbiased forward genetic and small molecule screens. Our central hypothesis is that forward screens performed using our zebrafish model of MYC-induced T-ALL will lead to the identification of entirely novel genes and pathways that play critical roles in MYC-induced leukemogenesis. However, zebrafish from our original line develop rapidly progressive T-ALL prior to achieving reproductive maturity, making this line poorly suited for the performance of large-scale screens. Therefore, a conditional model was required. We have now generated a transgenic zebrafish line that expresses a human MYC-estrogen receptor fusion construct under the control of the zebrafish recombination activating gene 2 (Rag2) promoter, which is lymphocyte-specific. When mated against fish transgenic for a Rag2-GFP transgene, the development and progression of T-ALL can be readily tracked in live fish by fluorescent microscopy. Upon treatment with 4-hydroxytamoxifen (4HT), zebrafish from this line develop fully penetrant T-ALL, with a mean time to tumor onset of 8 weeks. Additionally, removal from 4HT invariably led to complete morphologic remission in leukemic zebrafish from this line, and all of these fish remained alive and were able to mate successfully for greater than 6 months after removal from 4HT. This conditional zebrafish model of MYC-induced T-ALL will now allow the successful performance of forward genetic and small molecule screens to identify known and novel genes and pathways that play critical roles in T-ALL leukemogenesis and MYC-induced transformation. Figure Figure
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Chen, Lindsey N. "Cross-Linguistic Comparison of Screen Translations of Names in Hayao Miyazaki’s Animated Films." Вопросы Ономастики 18, no. 1 (2021): 195–208. http://dx.doi.org/10.15826/vopr_onom.2021.18.1.009.
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This article discusses the screen translation, into English and Chinese, of some of the names in Hayao Miyazaki’s animated films. In particular, in drawing onomastic examples from Miyazaki’s six animated fantasies, this study provides insights into the naming practices and strategies adopted by the screen translators into languages of distinct linguistic families. The analysis of names sorted into four categories yields the following results: (a) each screen translator used diff erent approaches to the translation of names, and (b) shared similarities with the source language and culture play a crucial role in the translation task. In brief, the first category concerns the films’ protagonists, for which the strategy of diminution is observed in Chinese but not in English translation. The second concerns the names of supporting human characters. Here, screen translators adopt several strategies, including direct phonetic transfer and incorporation of courtesy titles. The third comprises names of anthropomorphic and non-human creatures, and translated samples are shown more likely to be denotative and descriptive. Finally, there is no loss in translation with respect to the symbolic implication of location names. In general, screen translators utilized various linguistic strategies to produce onomastic substitutes that are acceptable to the local audience. Concurrently, they strived not to deviate too much from the original character names, in form and meaning.
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Natalie Winteringham, Louise, Raelene Endersby, Jennifer Beaumont, Jean-Philippe Lalonde, Merlin Crossley, and Svend Peter Klinken. "Hls5, a Novel Ubiquitin E3 Ligase, Modulates Levels of Sumoylated GATA-1." Blood 114, no. 22 (November 20, 2009): 253. http://dx.doi.org/10.1182/blood.v114.22.253.253.
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Abstract Abstract 253 Hemopoietic lineage commitment is controlled, in part, by transcription factors that regulate specific genes required for the formation of mature blood cells. Differentiation along particular hemopoietic lineages is dependant not only on the presence of particular transcription factors, but also on appropriate concentrations - altering transcription factor levels can force cells into different hemopoietic pathways. Transcription factors undergo numerous post-translational modifications and are controlled spatially via sub-cellular localisation. De-regulation of transcription factors can result in leukemias, or other blood disorders. GATA-1 is an example of a key lineage-determining gene, essential for erythropoiesis. Increasing GATA-1 levels promotes maturation along the erythroid pathway, whereas reducing GATA-1 concentrations favours myelopoiesis. GATA-1 regulation occurs at multiple levels including transcription, translation and post-translational modifications such as phosphorylation, acetylation, ubiquitination and sumoylation. Although GATA-1 ubiquitination modifies the protein for proteasomal degradation, the effect of adding small ubiquitin-like modier (Sumo) to GATA-1 is unclear. Several examples of hemopoietic differentiation plasticity have been observed. We reported a lineage switch by erythroleukemic J2E cells which spontaneously developed a monoblastoid phenotype. Two genes (Hls5 and Hls7/Mlf1) were isolated from this lineage switch with potential lineage-determining features. Hls5 is a member of the RBCC (Ring finger, B-box, Coiled-coil) family of proteins, which includes PML. Ectopic expression of Hls5 impedes erythroid differentiation by reducing GATA-1 levels, and suppressing hemoglobin synthesis. Significantly, Hls5 relocates from the cytoplasm to associate with GATA-1 in the nucleus, where it interferes with DNA binding and transactivation of GATA-1. Several members of the RBCC family are ubiquitin E3 ligases, catalysing the final step in the ubiquitination process - these molecules play a vital role in regulating the levels of target proteins. Here we show that Hls5 is a bona fide ubiquitin E3 ligase, in partnership with several ubiquitin E2 enzymes. The Ring finger is critical for Hls5 ligase activity as mutation of key residues within the Ring finger ablates catalytic activity. Interestingly, a yeast 2 hybrid screen for Hls5 interactors identified Ubc9 and Pias1, which act as E2 and E3 enzymes in the sumoylation cascade. Co-immunoprecipitation, BRET and co-localization experiments confirmed the Hls5 association with Ubc9 and Pias1. Moreover, Hls5 binds Sumo-1 (but not Sumo-2 or 3), and co-localizes with Sumo-1 in discrete nuclear bodies. Thus, Hls5 interacts with several components of the intracellular sumoylation machinery. Hls5 can also reduce sumoylated proteins globally, indicating it may target these modified proteins for degradation. Recently, a new family of ubiquitin E3 ligases has been described which specifically mark sumoylated proteins for degradation. These Sumo-targeted ubiquitin ligases (STUbL) are found primarily in yeast, and only one mammalian STUbL has been identified. We postulated that Hls5 may be a STUbL, capable of regulating sumoylated GATA-1. Our data demonstrate that while Hls5 is able to bind GATA-1 via the B-box and Coiled-coil domains, it preferentially associates with sumoylated GATA-1 through a canonical Sumo interacting motif (SIM). This results in increased GATA-1 ubiquitination and, as a consequence, levels of sumoylated GATA-1 are reduced substantially. Since mutation of the lysine necessary for Sumo attachment does not affect GATA-1 transactivation, sumoylation may act as a prelude to ubiquitination and protein turn-over. We propose, therefore, that GATA-1 mediates transcription of target genes, and is subsequently sumoylated by Pias1 and Ubc9 – addition of Sumo moieties to GATA-1 enhance binding to Hls5, which in turn impedes GATA-1 DNA binding, and promotes ubiquitination for proteasomal degradation. This model is consistent with decreased levels of GATA-1 in erythroid cells ectopically expressing Hls5, and with the original isolation of Hls5 as a potential lineage-determining gene involved with the erythroid to monoblastoid lineage switch. Thus, Hls5 is a novel STUbL which plays a role in hemopoietic lineage commitment by modulating GATA-1 activity and content. Disclosures: No relevant conflicts of interest to declare.
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Shorter, Edward. "Still tilting at windmills." Psychiatrist 35, no. 5 (May 2011): 183–84. http://dx.doi.org/10.1192/pb.bp.111.034108.
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SummaryThomas Szasz's essay misses several key points about the undoubted changes that psychiatry has undergone since he wrote his original screed against the discipline in 1961. Szasz fails to recognise that the discipline today acknowledges a neurological basis for much psychiatric illness. Thus, his fulminations against psychiatry for treating ‘mental illness' is off-base. Szasz's original diatribe was heavily against psychoanalysis. Yet today Freud's doctrines can scarcely be said to play even a marginal role in psychiatry, and it is absurd to keep levelling the same old charges of 50 years ago. One has the feeling of looking at one of the last veterans of the Esperanto movement in confronting Szasz: lunacy at the time, bizarrely outdated today.
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Arakkal, Rinshila. "From Birnamwood to Bollywood: A View of the Cinematographic Adaptation of Macbeth into Maqbool." International Journal of English and Comparative Literary Studies 1, no. 1 (November 22, 2020): 1–20. http://dx.doi.org/10.47631/ijecls.v1i1.144.
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Purpose: The study aims to explore the similarities and dissimilarities between William Shakespeare’s Macbeth and its film adaptation Maqbool by Vishal Bhardwaj. The study also aims to compare both the film and the play in terms of politics and power from a psychoanalytic perspective.
Methodology/ Approach: This study is based on thematic analysis and the main changes when the original play is adapted to film, in order to check the variation from stage to screen. Adaptation theory, Sigmund Freud’s psychoanalysis theory are used in this analysis. Bollywood movie Maqbool (2003) by director Vishal Bhardwaj and William Shakespeare’s great tragedy Macbeth (1606) are used as primary sources for this analysis.
Findings: The result of the analysis indicates that film and drama are entirely different. When an original play is adapted into film, there are many merits and demerits.Shakespeare mounded more on poetic language than on spectacle and other scenic devices to create the necessary emotional effect. The Elizabethan theatre gores were more audiences than spectators. But the modern spectators habituated to the computer-generated technique of cinematography expect something considerably different. The result is that when the text of the play is converted into a screenplay, there will be a remarkable reduction in the number of spoken words because mainstream cinema depends for its effect largely on visual rather than dialogue. However, the director maintained the originality of play despite the additions and reductions.
Conclusion: The paper throws light on the main changes from English Renaissance theatre to contemporary modern world or theatre. It depicts the Psychological behavioural differences and the power and political structures of the two different periods. The paper suggests that film adaptation is an effective and attractive tool to maintain the value and to understand the original text.
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Berni, Aurora, Lorenzo Maccioni, and Yuri Borgianni. "Observing Pictures and Videos of Creative Products: An Eye Tracking Study." Applied Sciences 10, no. 4 (February 21, 2020): 1480. http://dx.doi.org/10.3390/app10041480.
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The paper offers insights into people’s exploration of creative products shown on a computer screen within the overall task of capturing artifacts’ original features and functions. In particular, the study presented here analyzes the effects of different forms of representations, i.e., static pictures and videos. While the relevance of changing stimuli’s forms of representation is acknowledged in both engineering design and human-computer interaction, scarce attention has been paid to this issue hitherto when creative products are in play. Six creative products have been presented to twenty-eight subjects through either pictures or videos in an Eye-Tracking-supported experiment. The results show that major attention is paid by people to original product features and functional elements when products are displayed by means of videos. This aspect is of paramount importance, as original shapes, parts, or characteristics of creative products might be inconsistent with people’s habits and cast doubts about their rationale and utility. In this sense, videos seemingly emphasize said original elements and likely lead to their explanation/resolution. Overall, the outcomes of the study strengthen the need to match appropriate forms of representation with different design stages in light of the needs for designs’ evaluation and testing user experience.
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Eiring, Anna M., Ira L. Kraft, Brent D. G. Page, Tian Y. Zhang, Jamshid S. Khorashad, Nadeem A. Vellore, Kimberly R. Reynolds, et al. "BP5-087, a Novel STAT3 Inhibitor, Combines With BCR-ABL1 Inhibition To Overcome Kinase-Independent Resistance In Chronic Myeloid Leukemia." Blood 122, no. 21 (November 15, 2013): 854. http://dx.doi.org/10.1182/blood.v122.21.854.854.
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Abstract Mutations in the BCR-ABL1 kinase domain are a well-established mechanism of tyrosine kinase inhibitor (TKI) resistance, but fail to explain many cases of clinical TKI failure. In the remaining patients, resistance occurs via activation of alternative signaling pathways that maintain survival despite BCR-ABL1 inhibition (BCR-ABL1-independent resistance). STAT3 mediates TKI resistance in chronic myeloid leukemia (CML) cells cultured in the presence of bone marrow-derived factors (Bewry et al., 2008; Traer et al., 2012; Nair et al., 2012), and also plays a critical role in survival of CML cells with BCR-ABL1-independent resistance (Eiring et al. #31, ASH 2012). While targeting transcription factors is notoriously difficult, our combination of synthetic chemistry, in vitro reporter assays, and computational modeling has led to a low micromolar mechanism-based STAT3 inhibitor, which, in combination with TKIs, shows promise as a treatment for CML patients with BCR-ABL1-independent resistance. The original compound of the series, SF1-066 (10 µM; Fletcher et al., 2009), combines with TKIs to reduce survival of CML CD34+ cells exhibiting BCR-ABL1-independent resistance (Eiring et al. #31, ASH 2012). To improve the potency and selectivity of SF1-066, we synthesized successive STAT3 inhibitor libraries and ranked candidates by structure-activity relationship using a luciferase-based reporter screen (Kraft et al. #2445, ASH 2012). This reporter assay quantifies STAT3 transcriptional activity in TKI-resistant AR230R cells, which grow in the continuous presence of imatinib (1.0 µM), lack BCR-ABL1 kinase domain mutations, and exhibit high levels of pSTAT3Y705, thereby enabling convenient, high-throughput screening for potency and selectivity in the context of endogenous STAT3 activation. Among three sequential STAT3 inhibitor libraries, BP5-087 emerged as the new lead compound. Fluorescence polarization assays verified that BP5-087 was 5-fold more effective than SF1-066 in outcompeting an SH2 peptide probe, and computational simulations predicted better overall binding of BP5-087 (-9.6 kcal/mol) versus SF1-066 (-7.6 kcal/mol) to the STAT3 SH2 interface. In AR230R cell growth assays, BP5-087 was effective at a 5-fold lower dose compared to SF1-066, with minimal effects on TKI-sensitive parental controls. Therefore, we tested BP5-087 in the context of primary TKI resistance. BP5-087 (1 µM) in combination with imatinib (2.5 µM) reduced colony formation and increased apoptosis of CD34+ cells from CML patients with BCR-ABL1-independent resistance. These cells have no BCR-ABL1 kinase domain mutations and undergo BCR-ABL1 kinase inhibition as detected by immunoblot analyses. In contrast, BP5-087 had no effect on CD34+ cells from newly diagnosed CML patients or normal individuals. Immunofluorescence demonstrated that dual treatment of TKI-resistant CML CD34+ cells resulted in reduced levels of nuclear pSTAT3Y705, consistent with an inhibitor of STAT3 dimerization. In more primitive CML stem cells, long term culture-initiating cell (LTC-IC) assays revealed that neither inhibitor alone had any effect on colony formation of primitive LTC-IC progenitors, whereas imatinib (2.5 µM) in combination with BP5-087 (1.0 µM) reduced LTC-IC colony formation by 66%. Consistent with this observation, immunofluorescence showed high levels of pSTAT3Y705 in primitive TKI-resistant CD34+CD38- cells when cultured in the presence but not absence of TKIs. To test the feasibility of BP5-087 for in vivo use, we treated mice orally with BP5-087 (25 mg/kg/day) for 4 weeks and observed no changes in body weight, peripheral blood cellularity, or bone marrow colony forming ability. Mass spectrometry confirmed that BP5-087 is orally bioavailable. In summary, BP5-087 is a systematically-derived, direct inhibitor of STAT3 that, in combination with TKIs, reduces survival of CML cells with BCR-ABL1-independent resistance. Further rounds of structure-activity optimization may reveal an inhibitor with a clinically-relevant effective concentration. Disclosures: Deininger: Bristol Myers Squibb: Advisory Boards Other, Consultancy, Research Funding; Ariad Pharmaceuticals: Advisory Boards, Advisory Boards Other, Consultancy; Novartis: Advisory Boards, Advisory Boards Other, Consultancy, Research Funding; Celgene: Research Funding; Gilead Sciences: Research Funding.
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Jalili, Reza, Peter D. Kirchner, Jorge Montoya, Stephen Duncan, Luc Genevriez, James S. Lipscomb, Robert H. Wolfe, and Christopher F. Codella. "A Visit to the Dresden Frauenkirche." Presence: Teleoperators and Virtual Environments 5, no. 1 (January 1996): 87–94. http://dx.doi.org/10.1162/pres.1996.5.1.87.
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The Frauenkirche was destroyed when Dresden was bombed by the Allied forces February 13-14, 1945. The church is now being reconstructed in an effort led by the Foundation for the Reconstruction of the Frauenkirche. The VRDECK software package developed at the IBM T. J. Watson Research Center was used to view and walk through a model created from the original church plans. A Polhemus tracker and a custom-built joystick using the Logitech 3D mouse were used for six-degree-of-freedom input to the application. The interactive fly-through of the church is in an immersive environment. One can navigate around the model wearing a head-mounted display, sitting in front of a standard monitor, looking at a stereo image produced on a stereo monitor, or standing before a projection screen displaying a stereo image of the scene. The system was developed for and exhibited at the IBM booth in the CeBIT fair in Hannover, Germany in March 1994 with funding from IBM Germany.
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Gjærde, Line Klingen, Jane Hybschmann, Daniel Dybdal, Martha Krogh Topperzer, Morten Arnborg Schrøder, Jenny Louise Gibson, Paul Ramchandani, et al. "Play interventions for paediatric patients in hospital: a scoping review." BMJ Open 11, no. 7 (July 2021): e051957. http://dx.doi.org/10.1136/bmjopen-2021-051957.
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ObjectivePlay is a non-invasive, safe and inexpensive intervention that can help paediatric patients and their families manage difficult aspects of being ill or hospitalised. Although play has existed in hospitals for decades, research on hospital play interventions is scarce. This review aimed to categorise and synthesise the last 20 years of research on hospital play interventions.DesignScoping review.Data sourcesPubMed, CINAHL, CENTRAL, ERIC and PsycINFO (1 January 2000– 9 September 2020).Study selection and data extractionWe systematically searched for original peer-reviewed articles, written in English, on hospital play interventions in paediatric patients (0–18 years) in non-psychiatric settings. Two reviewers independently screened titles and abstracts, reviewed full text of relevant articles and extracted data. We thematically synthesised the data from the included studies, and a descriptive analysis, based on a developed framework, is presented.ResultsOf the 297 included articles, 78% came from high-income countries and 56% were published within the last 5 years. Play interventions were carried out across all ages by various healthcare professionals. Play interventions served different roles within four clinical contexts: A) procedures and diagnostic tests, B) patient education, C) treatment and recovery and D) adaptation. Across these contexts, play interventions were generally facilitated and purpose-oriented and had positive reported effects on pain, stress, and anxiety.ConclusionsPlay in hospitals is an emerging interdisciplinary research area with a significant potential benefit for child and family health. Future research should further describe principles for play in hospitals. High-quality studies investigating short-term and long-term effects are needed to guide when and how to best integrate play in hospitals.
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Monico, Letizia, Laura Cartechini, Francesca Rosi, Annalisa Chieli, Chiara Grazia, Steven De Meyer, Gert Nuyts, et al. "Probing the chemistry of CdS paints in The Scream by in situ noninvasive spectroscopies and synchrotron radiation x-ray techniques." Science Advances 6, no. 20 (May 2020): eaay3514. http://dx.doi.org/10.1126/sciadv.aay3514.
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The degradation of cadmium sulfide (CdS)–based oil paints is a phenomenon potentially threatening the iconic painting The Scream (ca. 1910) by Edvard Munch (Munch Museum, Oslo) that is still poorly understood. Here, we provide evidence for the presence of cadmium sulfate and sulfites as alteration products of the original CdS-based paint and explore the external circumstances and internal factors causing this transformation. Macroscale in situ noninvasive spectroscopy studies of the painting in combination with synchrotron-radiation x-ray microspectroscopy investigations of a microsample and artificially aged mock-ups show that moisture and mobile chlorine compounds are key factors for promoting the oxidation of CdS, while light (photodegradation) plays a less important role. Furthermore, under exposure to humidity, parallel/secondary reactions involving dissolution, migration through the paint, and recrystallization of water-soluble phases of the paint are associated with the formation of cadmium sulfates.
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Ilchenko, Sergey Nikolayevich. "«The White Guard» on TV: fascinated by masscult mythology." Journal of Flm Arts and Film Studies 6, no. 1 (March 15, 2014): 60–67. http://dx.doi.org/10.17816/vgik6160-67.
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The article analyzes the television adaptation of the famous novel by Mikhail Bulgakov, shown on Russian television in 2012. The author juxtaposes the TV-series with the renowned Soviet film Days of the Turbins, premiered in 1976. The analysis is carried out in the context of the history of a theatre version of the novel The White guard, staged in the Moscow Academic Art Theatre in the 1920s, that is Days of the Turbins, highly appreciated by Stalin. Traditionally, both theatre and cinema directors were drawn to the play adapted by Mikhail Bulgakov after his novel. Anyhow, there is a certain subject and semantic difference between these two works. The author analyzes the structure of the TV version, its style, elaboration of on-screen characters, based on the literary source and previous interpretation of the play Days of the Turbins. However, the author argues, that ideological and figurative interference into the original, adaptation to the stereotypes of mass culture significantly distort the perception of Bulgakovs works, largely obliged to the writerss mood and emotions experienced in the years of Revolution and Civil war. Concluding, the author pinpoints both - complexity unit of Bulgakovs text adaptation towards contemporary TV, and misjudgements of the TV-series makers in the way of conception and realization.
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Dickson, Bruce J. "Integrating Wealth and Power in China: The Communist Party's Embrace of the Private Sector." China Quarterly 192 (December 2007): 827–54. http://dx.doi.org/10.1017/s0305741007002056.
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AbstractIs privatization in China leading to political change? This article presents original survey data from 1999 and 2005 to evaluate the Communist Party's strategy towards the private sector. The CCP is increasingly integrating itself with the private sector, both by co-opting entrepreneurs into the Party and encouraging current Party members to go into business. It has opened the political system to private entrepreneurs, but still screens which ones are allowed to play political roles. Because of their close personal and professional ties, and because of their shared interests in promoting economic growth, China's capitalists and communist officials share similar viewpoints on a range of political, economic and social issues. Rather than promote democratic governance, China's capitalists have a stake in preserving the political system that has allowed them to prosper, and they are among the Party's most important bases of support.
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Puppo, Ronald. "Making room for small-language imports: Jacint Verdaguer." Babel. Revue internationale de la traduction / International Journal of Translation 56, no. 3 (October 28, 2010): 259–81. http://dx.doi.org/10.1075/babel.56.3.04pup.
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This essay discusses translation and translator choices in the context of importing a small-language poet into English-language circulation. Small-language imports play a modest but supporting role in counteracting the sort of cultural daltonism that screens any number of cultures and literatures from general global view. Taking the first English-language anthology of poetry by nineteenth-century Catalan-language poet Jacint Verdaguer (<i>Selected Poems of Jacint Verdaguer: A Bilingual Edition</i>, University of Chicago, 2007) as a case in point, the author/translator argues that if the native-culture value of poetic production is to be preserved in the new cultural and literary currency, contextual essentials (historical, social, literary) must be brought to light. Where the texts themselves are concerned, the translator of poetry must seek to re-create the form-content synthesis of the original, even as new intratextual and intertextual meanings and correspondences emerge in translation.
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Büttner, Fionn, Elaine Toomey, Shane McClean, Mark Roe, and Eamonn Delahunt. "Are questionable research practices facilitating new discoveries in sport and exercise medicine? The proportion of supported hypotheses is implausibly high." British Journal of Sports Medicine 54, no. 22 (July 22, 2020): 1365–71. http://dx.doi.org/10.1136/bjsports-2019-101863.
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Questionable research practices (QRPs) are intentional and unintentional practices that can occur when designing, conducting, analysing, and reporting research, producing biased study results. Sport and exercise medicine (SEM) research is vulnerable to the same QRPs that pervade the biomedical and psychological sciences, producing false-positive results and inflated effect sizes. Approximately 90% of biomedical research reports supported study hypotheses, provoking suspicion about the field-wide presence of systematic biases to facilitate study findings that confirm researchers’ expectations. In this education review, we introduce three common QRPs (ie, HARKing, P-hacking and Cherry-picking), perform a cross-sectional study to assess the proportion of original SEM research that reports supported study hypotheses, and draw attention to existing solutions and resources to overcome QRPs that manifest in exploratory research. We hypothesised that ≥ 85% of original SEM research studies would report supported study hypotheses. Two independent assessors systematically identified, screened, included, and extracted study data from original research articles published between 1 January 2019 and 31 May 2019 in the British Journal of Sports Medicine, Sports Medicine, the American Journal of Sports Medicine, and the Journal of Orthopaedic & Sports Physical Therapy. We extracted data relating to whether studies reported that the primary hypothesis was supported or rejected by the results. Study hypotheses, methodologies, and analysis plans were preregistered at the Open Science Framework. One hundred and twenty-nine original research studies reported at least one study hypothesis, of which 106 (82.2%) reported hypotheses that were supported by study results. Of 106 studies reporting that primary hypotheses were supported by study results, 75 (70.8%) studies reported that the primary hypothesis was fully supported by study results. The primary study hypothesis was partially supported by study results in 28 (26.4%) studies. We detail open science practices and resources that aim to safe-guard against QRPs that bely the credibility and replicability of original research findings.
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Browne, Dillon Thomas, Shealyn May, Pamela Hurst-Della Pietra, Dimitri Christakis, Tracy Asamoah, Lauren Hale, Katia Delrahim-Howlett, et al. "From ‘screen time’ to the digital level of analysis: protocol for a scoping review of digital media use in children and adolescents." BMJ Open 9, no. 11 (November 2019): e032184. http://dx.doi.org/10.1136/bmjopen-2019-032184.
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IntroductionResearch on the relationship between digital media exposure and child development is complex, inconsistent and fraught with debate. A highlighted area of inadequacy surrounds the methodological limitations of measuring digital media use for both researchers and clinicians, alike. This protocol aims to (1) identify core concepts in the area of screen time and digital media use in children and adolescents (2) map existing research paradigms and screening/measurement tools that serve to underpin and operationalise core concepts and (3) provide an initial step in integrating these findings into a consolidated screening toolkit. It is expected this enterprise will help advance research and clinical evaluation in fields concerned with digital media use, namely medicine, child development and the social sciences.Methods and analysisThe planned scoping review will search relevant electronic databases, including Ovid MEDLINE, PsycINFO and Scopus, in addition to grey literature. All empirical investigations and presentation of original research will be considered, and measurement/screening tools for digital media usage in children and adolescents will be identified and reported on. Two reviewers will pilot test the screening criteria, and data extraction forms prior to independently screening all relevant literature and extracting the data. A three-stage synthesis process will be used to map the existent measurement and screening tools for digital media usage in children and adolescents.Ethics and disseminationThere are no ethical considerations for this scoping review. Plans for dissemination include publication in a top-tier, open-access journal, public presentations and conference proceedings. Presentation of the full scoping review has been accepted to the American Academy of Child & Adolescent Psychiatry 66th Annual Meeting.
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Aprile, Francesca, Giovanni Bruno, Rossella Palma, Maria Teresa Mascellino, Cristina Panetta, Giulia Scalese, Alessandra Oliva, Carola Severi, and Stefano Pontone. "Microbiota Alterations in Precancerous Colon Lesions: A Systematic Review." Cancers 13, no. 12 (June 19, 2021): 3061. http://dx.doi.org/10.3390/cancers13123061.
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Gut microbiota plays an important role in human health. It may promote carcinogenesis and is related to several diseases of the gastrointestinal tract. This study of microbial dysbiosis in the etiology of colorectal adenoma aimed to investigate the possible causative role of microbiota in the adenoma–carcinoma sequence and its possible preventive role. A systematic, PRISMA-guided review was performed. The PubMed database was searched using “adenoma microbiota” and selecting original articles between January 2010 and May 2020 independently screened. A higher prevalence of Proteobacteria, Fusobacteria, and Bacteroidetes phyla was observed in the fecal luminal and mucosa-associated microbiota of patients with adenoma. However, other studies provided evidence of depletion of Clostridium, Faecalibacterium, Bacteroides and Romboutsia. Results on the relationship between adenoma endoscopic resection and microbiota were inconsistent. In conclusion, none of the analyzed studies developed a predictive model that could differentiate adenoma from non-adenoma patients, and therefore, to prevent cancer progression. The impact of adenoma’s endoscopic resection on microbiota was investigated, but the results were inconclusive. Further research in the field is required.
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Al-Batah, Mohammad Subhi, Belal Mohammad Zaqaibeh, Saleh Ali Alomari, and Mowafaq Salem Alzboon. "Gene Microarray Cancer Classification using Correlation Based Feature Selection Algorithm and Rules Classifiers." International Journal of Online and Biomedical Engineering (iJOE) 15, no. 08 (May 14, 2019): 62. http://dx.doi.org/10.3991/ijoe.v15i08.10617.
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Gene microarray classification problems are considered a challenge task since the datasets contain few number of samples with high number of genes (features). The genes subset selection in microarray data play an important role for minimizing the computational load and solving classification problems. In this paper, the Correlation-based Feature Selection (CFS) algorithm is utilized in the feature selection process to reduce the dimensionality of data and finding a set of discriminatory genes. Then, the Decision Table, JRip, and OneR are employed for classification process. The proposed approach of gene selection and classification is tested on 11 microarray datasets and the performances of the filtered datasets are compared with the original datasets. The experimental results showed that CFS can effectively screen irrelevant, redundant, and noisy features. In addition, the results for all datasets proved that the proposed approach with a small number of genes can achieve high prediction accuracy and fast computational speed. Considering the average accuracy for all the analysis of microarray data, the JRip achieved the best result as compared to Decision Table, and OneR classifier. The proposed approach has a remarkable impact on the classification accuracy especially when the data is complicated with multiple classes and high number of genes.
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Hao, Xinxin, Xiaoxue Li, and Jingchen Zheng. "Screening China Emergency Medical Team (CEMT) Members: A Self-Leadership Perspective." Prehospital and Disaster Medicine 33, no. 6 (October 31, 2018): 596–601. http://dx.doi.org/10.1017/s1049023x18000961.
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AbstractObjectiveThis study aims at establishing the self-leadership development model (SLM) of China Emergency Medical Team (CEMT) members as a supplement to current selection standards of CEMT members.MethodsRaw dataset was obtained through two ways: in-depth interviews and documentary materials (memoirs and articles). The in-depth interviews were conducted with a purposive sample of 12 CEMT members, all of whom have participated in multiple disaster relief activities and have been CEMT members for more than two years. This paper followed a grounded theory methodology dealing with all data.ResultsBased on tasks, the SLM-CEMT consists of three basic parts: (1) making plans; (2) action; and (3) outcomes. Different parts involve various self-leadership strategies, of which five are the original dimensions of previous research (goal-setting, visualizing successful performance, self-talk, self-reward, and self-correcting feedback) and three are new dimensions (role clarity, self-initiative, and self-vigilance).Conclusions:The SLM-CEMT, with the three new parts, provides a new look at screening CEMT members as well as pondering on future research. Based on the SLM-CEMT, administrators could screen more qualified CEMT members. For the limitations, future work will be on the generalization and confirmation of this model.HaoX,LiX,ZhengJ.Screening China Emergency Medical Team (CEMT) members: a self-leadership perspective.Prehosp Disaster Med.2018;33(6):596–601.
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Huang, Pinghua, and Xinyi Wang. "Piper-PCA-Fisher Recognition Model of Water Inrush Source: A Case Study of the Jiaozuo Mining Area." Geofluids 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/9205025.
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Source discrimination of mine water plays an important role in guiding mine water prevention in mine water management. To accurately determine water inrush source from a mine in the Jiaozuo mining area, a Piper trilinear diagram based on hydrochemical experimental data of stratified underground water in the area was utilized to determine typical water samples. Additionally, principal component analysis (PCA) was used for dimensionality reduction of conventional hydrochemical variables, after which mutually independent variables were extracted. The Piper-PCA-Fisher water inrush source recognition model was established by combining the Piper trilinear diagram and Fisher discrimination theory. Screened typical samples were used to conduct back-discriminate verification of the model. Results showed that 28 typical water samples in different aquifers were determined through the Piper trilinear diagram as a water sample set for training. Before PCA was carried out, the first five factors covered 98.92% of the information quantity of the original data and could effectively represent the data information of the original samples. During the one-by-one rediscrimination process of 28 groups of training samples using the Piper-PCA-Fisher water inrush source model, 100% correct discrimination rate was achieved. During the prediction and discrimination process of 13 samples, one water sample was misdiscriminated; hence, the correct prediscrimination rate was 92.3%. Compared with the traditional Fisher water source recognition model, the Piper-PCA-Fisher water source recognition model established in this study had higher accuracy in both rediscrimination and prediscrimination processes. Thus it had a strong ability to discriminate water inrush sources.
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Lagarde, Nathalie, Elodie Goldwaser, Tania Pencheva, Dessislava Jereva, Ilza Pajeva, Julien Rey, Pierre Tuffery, Bruno O. Villoutreix, and Maria A. Miteva. "A Free Web-Based Protocol to Assist Structure-Based Virtual Screening Experiments." International Journal of Molecular Sciences 20, no. 18 (September 19, 2019): 4648. http://dx.doi.org/10.3390/ijms20184648.
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Chemical biology and drug discovery are complex and costly processes. In silico screening approaches play a key role in the identification and optimization of original bioactive molecules and increase the performance of modern chemical biology and drug discovery endeavors. Here, we describe a free web-based protocol dedicated to small-molecule virtual screening that includes three major steps: ADME-Tox filtering (via the web service FAF-Drugs4), docking-based virtual screening (via the web service MTiOpenScreen), and molecular mechanics optimization (via the web service AMMOS2 [Automatic Molecular Mechanics Optimization for in silico Screening]). The online tools FAF-Drugs4, MTiOpenScreen, and AMMOS2 are implemented in the freely accessible RPBS (Ressource Parisienne en Bioinformatique Structurale) platform. The proposed protocol allows users to screen thousands of small molecules and to download the top 1500 docked molecules that can be further processed online. Users can then decide to purchase a small list of compounds for in vitro validation. To demonstrate the potential of this online-based protocol, we performed virtual screening experiments of 4574 approved drugs against three cancer targets. The results were analyzed in the light of published drugs that have already been repositioned on these targets. We show that our protocol is able to identify active drugs within the top-ranked compounds. The web-based protocol is user-friendly and can successfully guide the identification of new promising molecules for chemical biology and drug discovery purposes.
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Linh, Le Hung, Ta Hong Linh, Tran Dang Xuan, Le Huy Ham, Abdelbagi M. Ismail, and Tran Dang Khanh. "Molecular Breeding to Improve Salt Tolerance of Rice (Oryza sativa L.) in the Red River Delta of Vietnam." International Journal of Plant Genomics 2012 (December 27, 2012): 1–9. http://dx.doi.org/10.1155/2012/949038.
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Rice is a stable food in Vietnam and plays a key role in the economy of the country. However, the production and the cultivating areas are adversely affected from the threats of devastation caused by the rise of sea level. Using marker-assisted backcrossing (MABC) to develop a new salt tolerance rice cultivar is one of the feasible methods to cope with these devastating changes. To improve rice salt tolerance in BT7 cultivar, FL478 was used as a donor parent to introgress the Saltol QTL conferring salt tolerance into BT7. Three backcrosses were conducted and successfully transferred positive alleles of Saltol from FL478 into BT7. The plants numbers IL-30 and IL-32 in BC3F1 population expected recurrent genome recovery of up to 99.2% and 100%, respectively. These selected lines that carried the Saltol alleles were screened in field for their agronomic traits. All improved lines had Saltol allele similar to the donor parent FL478, whereas their agronomic performances were the same as the original BT7. We show here the success of improving rice salt tolerance by MABC and the high efficiency of selection in early generations. In the present study, MABC has accelerated the development of superior qualities in the genetic background of BT7.
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Han, Xiaoxu. "Infer Species Phylogenies Using Self-Organizing Maps." International Journal of Knowledge Discovery in Bioinformatics 1, no. 2 (April 2010): 29–49. http://dx.doi.org/10.4018/jkdb.2010040103.
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With rapid advances in genomics, phylogenetics has turned to phylogenomics due to the availability of large amounts of sequence and genome data. However, incongruence between species trees and gene trees remains a challenge in molecular phylogenetics for its biological and algorithmic complexities. A state-of-the-art gene concatenation approach was proposed to resolve this problem by inferring the species phylogeny using a random combination of widely distributed orthologous genes screened from genomes. However, such an approach may not be a robust solution to this problem because it ignores the fact that some genes are more informative than others in species inference. This paper presents a self-organizing map (SOM) based phylogeny inference method to overcome its weakness. The author’s proposed algorithm not only demonstrates its superiority to the original gene concatenation method by using same datasets, but also shows its advantages in generalization. This paper illustrates that data missing may not play a negative role in phylogeny inferring. This study presents a method to cluster multispecies genes, estimate multispecies gene entropy and visualize the species patterns through the self-organizing map mining.
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Gupta, Samrat, Saurabh Kumar, and Pradeep Kumar. "EVALUATING THE PREDICTIVE POWER OF AN ENSEMBLE MODEL FOR ECONOMIC SUCCESS OF INDIAN MOVIES." Journal of Prediction Markets 10, no. 1 (September 20, 2016): 30–52. http://dx.doi.org/10.5750/jpm.v10i1.1182.
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The Indian motion picture industry has experienced phenomenal growth during the last few decades and plays an important role in emerging economy of India. This paper integrates three analytical models in order to address the intriguing problem of revenue prediction of movies in Indian film industry. The paper attempts to investigate the determinants leading to the success of indigenous movies in Indian context. Ensemble model has been constructed by integrating the three analytical models (Neural Network, Classification and Regression Tree and Robust Regression) using linear optimization approach. Further, a four-way comparative analysis of these three models along with Ensemble model has been carried out. The predictive power of the models has been evaluated using four performance metrics namely root mean squared error (RMSE), mean absolute error (MAE), mean absolute percentage error (MAPE) and large prediction error (LPE). Analyzing novel and original data of 120 Indian movies released during the period August’06-October’15, this paper inspects the nitty-gritties of Indian film industry and seeks to explain the nuances. The study revealed that factors like hype generated on web by a movie, screens on which the movie is released, rating garnered by movie and its genre are the most influential variables in deciding the box-office performance of a movie. Further we observed, that the neural network model closely competes with ensemble model in terms of predictive accuracy. The ensemble model considerably reduces the predictive errors and yields better results on two of the performance metrics.
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Beidler, Philip D. "South Pacific and American Remembering; or, “Josh, We're Going to Buy This Son of a Bitch!”." Journal of American Studies 27, no. 2 (August 1993): 207–22. http://dx.doi.org/10.1017/s0021875800031534.
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For many post-1945 Americans, the title South Pacific does not describe a text so much as a process of remembering. For the cultural historian, it offers an account of production. It is the relationship of these that will be my subject. The pattern of the former will largely depend, of course, on the rememberer – something of a book perhaps, a play, a movie, a song, another song. The latter, albeit complicated, is traceable. It has a literary provenance in a 1947 collection of fictional narratives by James A. Michener entitled Tales of the South Pacific, variously described as short stories or a novel, but always noted as winner of the Pulitzer Prize. In translation to Rodgers and Hammerstein's 1949 Broadway classic, it adds another Pulitzer and a paperback fortune for the original honoree: to one of the most celebrated runs in the golden era of the American musical. It parlays that success into one of the first widely popular 33⅓ RPM long-playing records, memorialized in its dramatic connection by a cover photograph of Mary Martin and Ezio Pinza in the lead roles of U.S. Navy nurse Nellie Forbush and French planter Emile DeBecque. It then reappears as a 1958 movie musical spectacular, leading to another joyride of the Michener narrative on the best-seller lists, and to yet another best-selling LP, now in stereo, with the cover photo of the lovers, here Mitzi Gaynor and Rozzano Brazzi, resituated against a Hollywood backdrop of wide-screen tropical splendors. Along the way, it proliferates into endless road productions and revivals; television showings and VCR rentals; re-recordings and new recordings on LP, tape, and compact disk.
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Garcia, Taylor J., Jeffrey A. Brady, Kimberly A. Guay, James P. Muir, and William B. Smith. "190 Reduce Reuse ReRumen: Preservation method of rumen fluid collected from slaughtered cattle affects in vitro digestibility." Journal of Animal Science 97, Supplement_1 (July 2019): 66–67. http://dx.doi.org/10.1093/jas/skz053.150.
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Abstract Microbes and chemical constituents in the rumen fluid play an important role when re-establishing the rumen microbial population. Identification of a viable preservation method could have lasting implication of veterinary and research applications. Our objective was to determine the efficacy of preservation methods on rumen liquor. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Rumen content was collected from different locations within the rumen, strained through eight layers of cheesecloth, and divided into five 500-mL aliquots. Aliquots were randomly allocated to one of five preservation methods (freezing or lyophilisation) and preservative (glycerol; yes or no). A fresh control was maintained from each sample. Three reference feeds and two roughage sources were used in the batch culture procedure. Reference feeds were dried to a constant weight at 55°C and ground to pass through a 2-mm screen. Ground samples were subjected to batch culture in vitro true digestibility (IVTD) assays using each of the four ruminal fluids (plus the control) for digestibility estimates. Original samples and incubated residues were assayed for DM, CP, NDF, and ADF. Data were analyzed in a 2 × 2 + 1 augmented factorial design using GLIMMIX procedure of SAS. There were no differences (P > 0.05) in IVTD when using protein or energy feeds; however, there were differences in IVTD for all treatments for fiber concentrates and forage reference feeds. Despite lower degradation of feeds when frozen or lyophilized rumen fluid was used, preserved rumen fluid may be a viable option in the absence of fresh inoculum for veterinary and research applications
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Lau, Nancy, Alison O'Daffer, Susannah Colt, Joyce P. Yi-Frazier, Tonya M. Palermo, Elizabeth McCauley, and Abby R. Rosenberg. "Android and iPhone Mobile Apps for Psychosocial Wellness and Stress Management: Systematic Search in App Stores and Literature Review." JMIR mHealth and uHealth 8, no. 5 (May 22, 2020): e17798. http://dx.doi.org/10.2196/17798.
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Background In an oversaturated market of publicly available mobile apps for psychosocial self-care and stress management, health care providers, patients, and consumers interested in mental health–related apps may wonder which, if any, are efficacious. Readily available metrics for consumers include user popularity and media buzz rather than scientific evidence. Objective This systematic review aimed to (1) examine the breadth of therapeutic contents and features of psychosocial wellness and stress management apps available to self-help seekers for public download and (2) determine which of these apps have original research support. Methods First, we conducted a systematic review of publicly available apps on the iPhone App Store (Apple Inc) and Android Google Play (Google LLC) platforms using conventional self-help-seeking search terms related to wellness and stress. The results were limited to English-language apps available for free download. In total, 2 reviewers independently evaluated all apps and discussed the findings to reach 100% consensus regarding inclusion. Second, a literature review was conducted on the included apps to identify supporting studies with original data collection. Results We screened 3287 apps and found 1009 psychosocial wellness and stress management apps. Content varied widely. The most common evidence-based strategy was mindfulness-meditation, followed by positive psychology and goal setting. Most apps were intended to be used as self-help interventions, with only 1.09% (11/1009) involving an electronic therapist and 1.88% (19/1009) designed as a supplement to in-person psychotherapy. Only 4.66% (47/1009) of apps targeted individuals with psychological disorders, and less than 1% of apps (6/1009, 0.59%) targeted individuals with other chronic illnesses. Approximately 2% (21/1009, 2.08%) were supported by original research publications, with a total of 25 efficacy studies and 10 feasibility studies. The Headspace mindfulness app had the most evidence, including 8 efficacy studies. Most other scientifically backed apps were supported by a single feasibility or efficacy study. Conclusions Only 2.08% (21/1009) of publicly available psychosocial wellness and stress management mobile apps discoverable to self-help seekers have published, peer-reviewed evidence of feasibility and/or efficacy. Clinicians and investigators may use these findings to help patients and families navigate the volume of emerging digital health interventions for stress management and wellness.
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Ketabdar, Hamed, Amin Haji-Abolhassani, and Mehran Roshandel. "MagiThings." International Journal of Mobile Human Computer Interaction 5, no. 3 (July 2013): 23–41. http://dx.doi.org/10.4018/jmhci.2013070102.
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The theory of around device interaction (ADI) has recently gained a lot of attention in the field of human computer interaction (HCI). As an alternative to the classic data entry methods, such as keypads and touch screens interaction, ADI proposes a touchless user interface that extends beyond the peripheral area of a device. In this paper, the authors propose a new approach for around mobile device interaction based on magnetic field. Our new approach, which we call it “MagiThings”, takes the advantage of digital compass (a magnetometer) embedded in new generation of mobile devices such as Apple’s iPhone 3GS/4G, and Google’s Nexus. The user movements of a properly shaped magnet around the device deform the original magnetic field. The magnet is taken or worn around the fingers. The changes made in the magnetic field pattern around the device constitute a new way of interacting with the device. Thus, the magnetic field encompassing the device plays the role of a communication channel and encodes the hand/finger movement patterns into temporal changes sensed by the compass sensor. The mobile device samples momentary status of the field. The field changes, caused by hand (finger) gesture, is used as a basis for sending interaction commands to the device. The pattern of change is matched against pre-recorded templates or trained models to recognize a gesture. The proposed methodology has been successfully tested for a variety of applications such as interaction with user interface of a mobile device, character (digit) entry, user authentication, gaming, and touchless mobile music synthesis. The experimental results show high accuracy in recognizing simple or complex gestures in a wide range of applications. The proposed method provides a practical and simple framework for touchless interaction with mobile devices relying only on an internally embedded sensor and a magnet.
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Gillespie, Jenny, Adrienne Hughes, Ann-Marie Gibson, Jess Haines, Elsie Taveras, and John J. Reilly. "Protocol for Healthy Habits Happy Homes (4H) Scotland: feasibility of a participatory approach to adaptation and implementation of a study aimed at early prevention of obesity." BMJ Open 9, no. 6 (June 2019): e028038. http://dx.doi.org/10.1136/bmjopen-2018-028038.
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IntroductionPrevention of childhood obesity is an important public health objective. Promoting healthful energy balance related behaviours (EBRBs) in the early years should be a key focus. In Scotland, one in five children are overweight or obese by age 5 years, with levels highest in deprived areas. This study protocol outlines the stages of a feasibility study to translate the highly promising North American Healthy Habits, Happy Homes (4H) a home based, preschool childhood obesity prevention intervention to Scotland (4H Scotland). First, elements of participatory and co-production approaches utilised to: (a) engage key stakeholders, (b) enable inclusive recruitment of participants and (c) adapt original study materials. Second, 4H Scotland intervention will be tested within a community experiencing health/social inequalities and high levels of deprivation in Dundee, Scotland.Methods and analysis4H Scotland aims to recruit up to 40 families. Anthropometry, objective and subjective measures of EBRBs will be collected at baseline and at 6 months. The intervention consists of monthly visits to family home, using motivational interviewing and SMS to support healthful EBRBs: sleep duration, physical activity (active play), screen time, family meals. The Control Group will receive standard healthy lifestyle information. Fidelity to intervention will be assessed using recordings of intervention visits. Feasibility and acceptability of study design components will be assessed through qualitative interviews and process evaluation of recruitment, retention rates; appropriateness, practicality of obtaining outcome measures; intervention duration, content, mode of delivery and associated costs. Adaptation through participatory and co-production will support development of 4H Scotland. Process evaluation offers two future directions; advancement towards a definitive, larger trial or routine practice.Ethics and disseminationThis study was granted ethical approval by the University of Strathclyde’s School of Psychological Sciences and Health Ethics Committee. Results will be disseminated through lay summaries workshops, peer-reviewed publications and conference presentations.Trial registration numberISRCTN13385965; Pre-results.
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Reuther, Gary W., Que T. Lambert, Michael A. Caligiuri, and Channing J. Der. "Identification and Characterization of an Activating TrkA Deletion Mutation in Acute Myeloid Leukemia." Molecular and Cellular Biology 20, no. 23 (December 1, 2000): 8655–66. http://dx.doi.org/10.1128/mcb.20.23.8655-8666.2000.
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ABSTRACT In this study, we utilized retroviral transfer of cDNA libraries in order to identify oncogenes that are expressed in acute myeloid leukemia (AML). From screens using two different cell types as targets for cellular transformation, a single cDNA encoding a variant of theTrkA protooncogene was isolated. The protein product of this protooncogene, TrkA, is a receptor tyrosine kinase for nerve growth factor. The isolated transforming cDNA encoded a TrkA protein that contains a 75-amino-acid deletion in the extracellular domain of the receptor and was named ΔTrkA. ΔTrkA readily transformed fibroblast and epithelial cell lines. The deletion resulted in activation of the tyrosine kinase domain leading to constitutive tyrosine phosphorylation of the protein. Expression of ΔTrkA in cells led to the constitutive activation of intracellular signaling pathways that include Ras, extracellular signal-regulated kinase/mitogen-activated protein kinase, and Akt. Importantly, ΔTrkA altered the apoptotic and growth properties of 32D myeloid progenitor cells, suggesting ΔTrkA may have contributed to the development and/or maintenance of the myeloid leukemia from which it was isolated. Unlike Bcr-Abl, expression of ΔTrkA did not activate Stat5 in these cells. We have detected expression of ΔTrkA in the original AML sample by reverse transcriptase PCR and by Western blot analysis. While previous TrkA mutations identified from human tumors involved fusion to other proteins, this report is the initial demonstration that deletions within TrkA may play a role in human cancers. Finally, this report is the first to indicate mutations in TrkA may contribute to leukemogenesis.
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Kilsdonk, Melvin, Sabine Siesling, Renee Otter, and Wim van Harten. "Evaluating the impact of accreditation and external peer review." International Journal of Health Care Quality Assurance 28, no. 8 (October 12, 2015): 757–77. http://dx.doi.org/10.1108/ijhcqa-05-2014-0055.
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Purpose – Accreditation and external peer review play important roles in assessing and improving healthcare quality worldwide. Evidence on the impact on the quality of care remains indecisive because of programme features and methodological research challenges. The purpose of this paper is to create a general methodological research framework to design future studies in this field. Design/methodology/approach – A literature search on effects of external peer review and accreditation was conducted using PubMed/Medline, Embase and Web of Science. Three researchers independently screened the studies. Only original research papers that studied the impact on the quality of care were included. Studies were evaluated by their objectives and outcomes, study size and analysis entity (hospitals vs patients), theoretical framework, focus of the studied programme, heterogeneity of the study population and presence of a control group. Findings – After careful selection 50 articles were included out of an initial 2,025 retrieved references. Analysis showed a wide variation in methodological characteristics. Most studies are performed cross-sectionally and results are not linked to the programme by a theoretical framework. Originality/value – Based on the methodological characteristics of previous studies the authors propose a general research framework. This framework is intended to support the design of future research to evaluate the effects of accreditation and external peer review on the quality of care.
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Iqbal, Naeem, Rashid Ahmad, Faisal Jamil, and Do-Hyeun Kim. "Hybrid features prediction model of movie quality using Multi-machine learning techniques for effective business resource planning." Journal of Intelligent & Fuzzy Systems 40, no. 5 (April 22, 2021): 9361–82. http://dx.doi.org/10.3233/jifs-201844.
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Quality prediction plays an essential role in the business outcome of the product. Due to the business interest of the concept, it has extensively been studied in the last few years. Advancement in machine learning (ML) techniques and with the advent of robust and sophisticated ML algorithms, it is required to analyze the factors influencing the success of the movies. This paper presents a hybrid features prediction model based on pre-released and social media data features using multiple ML techniques to predict the quality of the pre-released movies for effective business resource planning. This study aims to integrate pre-released and social media data features to form a hybrid features-based movie quality prediction (MQP) model. The proposed model comprises of two different experimental models; (i) predict movies quality using the original set of features and (ii) develop a subset of features based on principle component analysis technique to predict movies success class. This work employ and implement different ML-based classification models, such as Decision Tree (DT), Support Vector Machines with the linear and quadratic kernel (L-SVM and Q-SVM), Logistic Regression (LR), Bagged Tree (BT) and Boosted Tree (BOT), to predict the quality of the movies. Different performance measures are utilized to evaluate the performance of the proposed ML-based classification models, such as Accuracy (AC), Precision (PR), Recall (RE), and F-Measure (FM). The experimental results reveal that BT and BOT classifiers performed accurately and produced high accuracy compared to other classifiers, such as DT, LR, LSVM, and Q-SVM. The BT and BOT classifiers achieved an accuracy of 90.1% and 89.7%, which shows an efficiency of the proposed MQP model compared to other state-of-art- techniques. The proposed work is also compared with existing prediction models, and experimental results indicate that the proposed MQP model performed slightly better compared to other models. The experimental results will help the movies industry to formulate business resources effectively, such as investment, number of screens, and release date planning, etc.
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Castaldo, Matteo, Antonella Catena, César Fernández-de-las-Peñas, and Lars Arendt-Nielsen. "Widespread Pressure Pain Hypersensitivity, Health History, and Trigger Points in Patients with Chronic Neck Pain: A Preliminary Study." Pain Medicine 20, no. 12 (March 28, 2019): 2516–27. http://dx.doi.org/10.1093/pm/pnz035.
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Abstract Background Pain sensitivity in chronic neck pain patients may be influenced by health conditions related to higher levels of widespread pressure pain hypersensitivity (sensitization). Trigger points have also been reported to play a role in the sensitization process. Objectives To investigate the association between pressure pain thresholds, trigger points, and health conditions in patients with chronic neck pain. Design Original research, preliminary study. Setting A private clinic. Subjects Thirty-four chronic mechanical neck pain patients and 34 chronic whiplash-associated neck pain patients, giving a final sample of 68 chronic neck pain patients. Methods Patients underwent an assessment of pressure pain thresholds over the upper trapezius, extensor carpi radialis longus, and tibialis anterior muscles and were screened for the presence of trigger points in the upper trapezius muscle. Further, information about health history conditions was obtained and collected in a form. Results Significantly negative correlations between all pressure pain thresholds and duration of health history conditions were found (all P < 0.02). Significantly lower pressure pain thresholds (all P < 0.01) were found in patients with active trigger points as compared with those with latent trigger points. Conclusion Widespread pressure pain hypersensitivity was associated with duration of health history conditions, suggesting that long-lasting health complaints may act as a triggering/perpetuating factor, driving sensitization in individuals with chronic neck pain. Active trigger points may be associated with higher widespread pressure hypersensitivity.
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Chen, Luxi, Long Yi, Yuning Ren, Jianying Zhang, A. Douglas Kinghorn, Michael A. Caligiuri, and Jianhua Yu. "Enhancement of Natural Killer Cell Interferon-Gamma Production By the Derivatives of the Natural Product Phyllanthusmins Via TLR-Mediated NF-Kb and STAT3 Signaling Pathways." Blood 126, no. 23 (December 3, 2015): 1031. http://dx.doi.org/10.1182/blood.v126.23.1031.1031.
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Abstract Natural killer (NK) cells are known to play a pivotal role in regulating immune defense against tumors and viruses. However, very few studies describe the effects of natural products or their derivatives on NK cell function. We previously showed that the natural product lignan phyllanthusmin C enhances human NK cell IFN-gamma production. In this study, we successfully synthesized dozens of phyllanthusmin derivatives and screened their activities to enhance IFN-gamma production by NK cells. We found that three of these derivatives possessed capacities to induce IFN-gamma production by NK cells. Among them, derivative No. 17 had the highest efficacy and was significantly superior (P < 0.05) to that of the original phyllanthusmin C. These three phyllanthusmin derivatives had no adverse effect on human NK cell survival or proliferation. All derivatives alone or in combination with IL-12 or IL-15 induced human NK cell interferon (IFN)-gamma in comparison with the corresponding vehicle control or cytokine alone (P < 0.05). These derivatives stimulated IFN-gamma production in both CD56bright and CD56dim human NK cell subsets (P < 0.01). Mechanistically, immunoblotting assays and chromatin immunoprecipitation (ChIP) analyses combined with promoter-reporter luciferase assays revealed that these phyllanthusmin derivatives induced the phosphorylation of NF-κB and STAT3, resulting in their increased binding on theIFN G promoter, which was dependent on the Toll-like receptor (TLR) 1 and the TLR3 signaling pathways, respectively. STAT3 knockdown with lentivirus shRNA and inhibition of NF-kB signaling with a specific inhibitor (TPCK) significantly attenuated IFN-gamma production induced by these phyllanthusmin derivatives in human NK cells (P < 0.05). Blockade of TLR1 or TLR3 with their corresponding neutralizing antibodies nearly abolished activation of NF-κB or STAT3 as well as IFN-γ induction by the phyllanthusmin derivatives (P < 0.05). In conclusion, we have successfully synthesized and screened phyllanthusmin derivatives and identified three compounds that induce IFN-gamma production in human NK cells, one of which had optimal potential. The induction of human NK cell IFN-gamma production by phyllanthusmin derivatives is mediated by TLR1-NF-κB and TLR3-STAT3 signaling pathways, rendering their potential for use as agents for cancer prevention or treatment. Disclosures No relevant conflicts of interest to declare.
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Turnacioglu, K. K., B. Mittal, G. A. Dabiri, J. M. Sanger, and J. W. Sanger. "An N-terminal fragment of titin coupled to green fluorescent protein localizes to the Z-bands in living muscle cells: overexpression leads to myofibril disassembly." Molecular Biology of the Cell 8, no. 4 (April 1997): 705–17. http://dx.doi.org/10.1091/mbc.8.4.705.
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Cultures of nonmuscle cells, skeletal myotubes, and cardiomyocytes were transfected with a fusion construct (Z1.1GFP) consisting of a 1.1-kb cDNA (Z1.1) fragment from the Z-band region of titin linked to the cDNA for green fluorescent protein (GFP). The Z1.1 cDNA encodes only 362 amino acids of the approximately 2000 amino acids that make up the Z-band region of titin; nevertheless, the Z1.1GFP fusion protein targets the alpha-actinin-rich Z-bands of contracting myofibrils in vivo. This fluorescent fusion protein also localizes in the nascent and premyofibrils at the edges of spreading cardiomyocytes. Similarly, in transfected nonmuscle cells, the Z1.1GFP fusion protein localizes to the alpha-actinin-containing dense bodies of the stress fibers in vivo. A dominant negative phenotype was also observed in living cells expressing high levels of this Z1.1GFP fusion protein, with myofibril disassembly occurring as titin-GFP fragments accumulated. These data indicate that the Z-band region of titin plays an important role in maintaining and organizing the structure of the myofibril. The Z1.1 cDNA was derived from a chicken cardiac lambda gt11 expression library, screened with a zeugmatin antibody. Recent work has suggested that zeugmatin is actually part of the N-terminal region of the 81-kb titin cDNA. A reverse transcriptase polymerase chain reaction using a primer from the distal end (5' end) of the Z1.1 zeugmatin cDNA and a primer from the nearest known proximal (3' end) chicken titin (also called connectin) cDNA resulted in a predicted 0.3-kb polymerase chain reaction product linking the two known chicken titin cDNAs to each other. The linking region had a 79% identity at the amino acid level to human cardiac titin. This result and a Southern blot analysis of chicken genomic DNA hybridized with Z1.1 add further support to our original suggestion that zeugmatin is a proteolytic fragment from the N-terminal region of titin.
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Zhang, Wei Hua, Jian Hua Zhang, and Rui Li. "Assessment about Development Level of Low-Carbon Economy in Resource-Based City." Applied Mechanics and Materials 675-677 (October 2014): 1756–59. http://dx.doi.org/10.4028/www.scientific.net/amm.675-677.1756.
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In recent years, the phenomenon that global is becoming warmer, natural disasters occur frequently, natural resources and energy are decreasing more and more seriously affects the survival of mankind and the normal production and other activities. In this context, the concepts of “low-carbon economy”, “low-carbon city”, and “low-carbon technology” come into world attention, and low-carbon economy become the main direction of transformation of the resource-based city gradually. Therefore, studying problems about developmental level evaluation and strategies of low-carbon economy for the resource-based city has become an important research topic of scholars both at home and abroad.Facing the low-carbon economic development model, under the background of fully understanding the research status of the resource-based cities at home and abroad and low-carbon economy, the author sum up some problems which the resource-based cities need facing in sustainable development, and put forward concrete solving countermeasures. In order to guarantee the low-carbon economy developing better, the author found a low-carbon economy evaluation index system which suit for the resource-based city, on the base of following the principle of low-carbon economy evaluation index system setting up and the latest evaluation standard. Then taking Daqing city as an example, the author analyses the status of economic industry development in Daqing city and the obstacles existing in the low-carbon economy development of Daqing. At last, it screens 34 secondary indexes, and applies FAHP and DEA method to evaluation. The model gives full play to the features that FAHP can effectively reduce the index weight of subjectivity and DEA is easy to operate and can adjust to the original inputs, and provides theoretical guidance for resource-based cities develop low-carbon economy. Finally, the author submits specific promoting strategies for the resource-based city develop low-carbon economy, which can offer references for the sustainable development of the resource-based city.
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Muracki, Jarosław, Sebastian Klich, Adam Kawczyński, and Shellie Ann Boudreau. "Injuries and Pain Associated with Goalkeeping in Football—Review of the Literature." Applied Sciences 11, no. 10 (May 19, 2021): 4669. http://dx.doi.org/10.3390/app11104669.
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Knowledge and research about football goalkeeper (GK) injuries are scarce, which prevents the development of evidence-based injury prevention programs. Fortunately, progress is evident in injury prevention strategies in outfield football players. However, a GK fulfills a unique role, and an injured GK can substantially impact a team. Thus, there is a need to clarify and summarize current knowledge concerning football goalkeeper pain and injuries. This narrative review aims to present the best-evidence synthesis of knowledge about football GK injuries and pain, their type, location, and incidence. A secondary aim is to contrast these findings with outfield players and identify knowledge gaps. Scientific databases were searched for the following indexed terms: goalkeeper, injury, soccer, and football. Original papers, including case studies and systematic reviews published from August 1994 to March 2021, were screened for relevance using a priori criteria and reviewed. Commonly described injuries are fractures, luxation and dislocations in the fingers, hand and wrist. The quadriceps femoris and forearms muscles are the most frequently described muscle and tendon injuries. Further, football GK injuries differ in type, location, and incidence compared to outfield players. Whether GKs suffer fewer injuries than players in other positions, whether GK suffer more injuries in training than matches, and whether they sustain more upper limb injuries comparing to field players is still unclear and controversial. Few studies assess pain, and current data point to the development of hip and groin, thigh, knee, arm and forearm pain resulting from training and match play. Due to the crucial role of GK in the football team, it is recommended to use the injury burden as a parameter considering the number and time-loss of injuries in future studies.
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Boughanem, Hatim, Silvia Canudas, Pablo Hernandez-Alonso, Nerea Becerra-Tomás, Nancy Babio, Jordi Salas-Salvadó, and Manuel Macias-Gonzalez. "Vitamin D Intake and the Risk of Colorectal Cancer: An Updated Meta-Analysis and Systematic Review of Case-Control and Prospective Cohort Studies." Cancers 13, no. 11 (June 4, 2021): 2814. http://dx.doi.org/10.3390/cancers13112814.
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Obesity, a sedentary lifestyle, high red meat consumption and alcohol, and tobacco are considered the driving factors behind colorectal cancer (CRC) worldwide. Both diet and lifestyle are recognized to play an important role in the prevention of CRC. Forty years later, the vitamin D–cancer hypothesis is considered consistent. However, the relationship between low vitamin D intake and CRC is still controversial. The aim of this meta-analysis is to determine the associations between Vitamin D intake and CRC. MEDLINE-PubMed and Cochrane databases were searched up to May 2020 for studies evaluating the association between vitamin D intake (from foods and supplements) and CRC. Two reviewers, working independently, screened all titles and abstracts to identify the studies that met the inclusion criteria (case-control or prospective cohort (PC) studies published in English). Data were pooled by the generic inverse variance method using a random or fixed effect model. Heterogeneity was identified using the Cochran Q-test and quantified by the I2 statistic. A total of 31 original studies were included for the quantitative meta-analysis, comprising a total 47.540 cases and 70.567 controls in case-control studies, and a total of 14.676 CRC-incident cases (out of 808.130 subjects in PC studies) from 17 countries. A significant 25% lower risk was reported comparing the highest vs. the lowest dietary vitamin D consumption and CRC risk (odds ratio (95% confidence interval): 0.75 (0.67; 0.85)) in case-control studies, whereas a non-significant association was reported in case of prospective studies (hazard ratio (95% confidence interval): 0.94 (0.79; 1.11). The present meta-analysis demonstrates that high dietary vitamin D is associated to CRC prevention. However, larger and high-quality prospective studies and clinical trials are warranted to confirm this association.
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Nikoriak, Nataliia. "Text on the Culturological Border: the Cinemanovel “The Red. Without a Front Line” by A. Kokotiukha." Pitannâ lìteraturoznavstva, no. 102 (December 28, 2020): 164–94. http://dx.doi.org/10.31861/pytlit2020.102.164.
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The article under studies reveals the terminological polymodality of the concept of “cinemanovel” as a screened novel; film genre; an original narrative work that tends to a screenplay; literary text written on the basis of the film and the screenplay to it (film “novelization”). An overview of modern theoretical and practical discourse of the cinemanovel genre is presented in the paper. It has been emphasized that some researchers try to find out the origins of this genre by analyzing the samples in a comparative and intermediate way, while others focus on clarifying the specifics of individual novels, concluding on the synthetic and hybrid nature of this genre. In particular, in this aspect, the cinemanovel-prequel by A. Kokotiukha “The Red. Without a Front Line” (2019) has been analyzed. This text, based on a film screenplay, appears to be a rather complex construct that acquires a double coding – cinematic and literary – hence the genre of the novel (as a product of the synthesis of two arts) contains the key features of both. On the one hand, we have to deal with the preservation of the cinematic codes that pass from the screenplay: fragmentation, word visualization, documentalism, eventfulness, editing, alternation of angles and plans, time reduction, dialogues, character formation in action, characterization through speech, conciseness of phrases in certain scenes to create the effect of maximum tension, image condensation, accumulation of internal tension in the episode. On the other hand (as a result of the so-called “novelization”), the text acquires genre features of the novel. These are: the scale of the narration (although fragmented and condensed), the description of characters’ lives is presented in line with historical events, with the disclosure of their psychology and inner world. Finally, the work is also marked with specifically architectonic, i.e. the author connects his cinemanovels together by means of a plot, the main character and a general artistic idea.
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Kalenichenko, O. M. "Interpretation of Gogol’s works on the puppet theater stage (based on the spectacle by Oksana Dmitrieva «May night, or Moonlight Witchcraft»)." Aspects of Historical Musicology 17, no. 17 (September 15, 2019): 148–62. http://dx.doi.org/10.34064/khnum2-17.10.
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Background. M. Gogol’s «Evenings on a Farm Near Dikanka» often attract the attention of theater directors. Thus, in June 2009, the premiere of the play «May night, or Moonlight Witchcraft» directed by Oksana Dmitrieva, took place at the Kharkov Puppet Theater. Trying to reveal the genre nature of the production, theater critics give it such definitions as a fairy tale, musical, fantasy, ethno-folk show, liturgy, mystery play, as well as analyze individual finds of a young director, but the complete picture of the artistic features of this performance is absent yet. In this regard, the purpose of the article is to identify the features of the interpretation of the Gogol story by director O. Dmitrieva. Results. The «May night...» begins with a musical introduction consisting of two themes: the lyrical theme of the pipe with intonations of Transcarpathian melodies (which is connected with the young couple Hanna and Levko and the image of Pannochka) and the theme of hand drums, which reveals the inner strength of the Ukrainian people, as well as demonological beginning associated with the witch-stepmother. The music gives way to the sounds of night nature and the stars appear on the backdrop. Their low location and shape resemble the Christmas stars, with which carolers sing for Christmas. In the dark, the figure of Pannochka appears, wrapped in white cloths remembering a shroud. The unfolding of intersecting clothes above Pannochka’s head, and then their rotation symbolize both the alternation of day and night and the winter solstice. Thus, there are both, the Orthodox and the Pagan features, in depiction of the Ukrainian village. From several notes that the heroine sings, her leitmotif grows up. He fits well on modern arrangements of Ukrainian music, and is easily recognizable on his own. In combination with Pannochka’s sudden gusty movements (as if a bird is trying to break out of the snare, fly up into the sky), it helps to reveal her ambivalent nature: on the one hand, of the martyr, on the other – the representative of evil forces. Pannochka becomes the main character of the performance, and the Moon becomes her attribute, which can turn into the tambourine of shaman, the lyre, the sword, etc. The youth walking scene “on the garden” with the use of the jigging puppet, accompanied by folk songs differs in tempo and rhythm from previous mysteriously lyrical scenes. In the next episode, Pannochka enchants the characters on the stage with moonlight, so the meeting and the dialogue between Hanna and Levko begin to be perceived as a dream of heroes. This is facilitated by both the slow movements of the actors, the lengthy summons into the names of the characters, their flight around the stage, and the dialogue with the Moon that Pannochka props up. The tragic history of Pannochka is depicted first with the help of portraits of its participants on round screens, and then the screens are assembled into the figure of a Witch-Cat. This form also is reminiscent of a Chinese dancing Dragon. The episode with the hand fans depicting the “cat’s claws” is accompanied by alarming drum sound: Pannochka has no repose from the Witch even after death. The village in the new picture is reflected in the ripples of water: the real world is floating, swinging. Hanna and Levko confess their love to each other, however, Kalenik suddenly appears, recalling the Head. The image of the Head is solved by the director using two masks – large and small. At the beginning of the second act, the actors appear on the stage with long poles, which are similar both to the Chinese combat weapon and to the Ukrainian musical instruments “trembits”, allowing the actors to show brilliant plastic technique of “slow-motion”. Stylized masks of animals (cows, goats, pigs, roosters), which the walking lads pulling on themselves are the allusion to the Christmas fests. The lad boys strive to annoy the Head, so Head masks reappear on the scene, but there are already three of them: large, medium and small. With their help, there is a debunking of this character losing his power. The action transferred to the bottom of the pond, as symbolized by stylized fish. The drums and the fans – the cat’s claws – once again remind of the conflict between Pannochka and the Witch. Like in Gogol’s novella, the heroine asks Levko to find the Stepmother-Witch. The marionnette a la planchette and then – a shadow paper doll represent the image of the hero. Thanks to Levko, Mermaids (the original puppets) seize the Witch, and her death is symbolized by a broken rattle-rattle with the image of the cat’s muzzle. Next, the scene action follows by the Gogol’s novella: grateful Pannochka given to Levko the note, Head read it and allowed his son to marry Hanna. The image of Levko is represented here both in the system of the tablet puppet and in the means of the shadow theater. And the long clothes-shrouds acquainted from the first episodes of the play perform a number of new functions: this is the water of the pond, where Pannochka floats, and the paper, on which the note is written, and later – the wedding table. In this way the end of the Pannochka plot line comes. The spiritual verse «The soul with the body was parting» sounds, and in the hands of actress V. Mishchenko, the light paper doll, as the soul of her heroine, seeks up into the sky. Pannochka redeemed her sins, and now her soul can fly to heaven, because Easter has come. The last episode uses the “time-lapse” technique symbolizing the cleansing of the world from evil, and Pannochka’s leitmotif is organically superimposed on the Easter chime of bells. The action ends with a rap on the words “The Angels had opened the windows and they are looking on us” and the news that Easter has come. The final supports an idea that a person’s life moves from Christmas to Easter, from suffering to light, thus closing the spectacle into a ring composition. Conclusions. The original Gogol’s text allowed O. Dmitrieva to show a wide palette of modern possibilities of the puppet theater and the high skill of the actors of the “live plan”. In addition, the interweaving of national and foreign, Orthodoxy and paganism, an appeal to the expressive possibilities of the Ukrainian folk and modern music and to the ballet plastique suggest the postmodern nature of the play «May night, or MoonlightWitchcraft».
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Beaumont, Tim, Martijn Kedde, Sabrina Merat, Mark Kwakkenbos, Lina Bartels, Dorien van der Berg, Koen Wagner, et al. "499 AT1636, a colon cancer survivor-derived antibody recognizes a previously unidentified truncated, O-mannosylated 70kDa variant of E-cadherin." Journal for ImmunoTherapy of Cancer 8, Suppl 3 (November 2020): A534. http://dx.doi.org/10.1136/jitc-2020-sitc2020.0499.
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BackgroundColorectal cancer (CRC) associated with Lynch syndrome is characterized by an abundance of infiltrating lymphocytes. To study whether tumor-specific antibodies with therapeutic potential can be isolated from these patients, the B-cell repertoire from a patient with Lynch syndrome who recovered from a stage IV colon carcinoma was screened. Here we describe an antibody, AT1636 that recognizes a previously unidentified O-mannosylated 70kDa form of E-cadherin. The intercellular interactions by E-cadherin on tumor cells have for long been recognized as protective in cancer metastasis, and deregulation of E-cadherin is a hallmark for epithelial-mesenchymal transition (EMT).MethodsThe study protocol was approved by the Medical Ethical Committee of the Academic Medical Centre, Amsterdam, The Netherlands (NL42718.018.12). AIMM’s BCL6 and Bcl-xL immortalization method1 was used to interrogate the human antibody repertoire. From a carrier of a pathogenic gene variant in the MSH6 gene diagnosed with stage IV CRC and liver metastasis that had been treated with avastin, capecitabine and oxaliplatin, peripheral-blood memory B cells were obtained 9 years after last treatment. Antibodies-containing supernatant of cultured B-cells were screened for binding to 3 different CRC cell lines (DLD1, LS174T and COLO205) and absence of binding to fibroblast by flow cytometry. A high-affinity variant of AT1636 (AT1636IYN) was sorted from the original AT1636, AID-expressing B-cell clone.2ResultsAntibodies that demonstrated differential binding to CRC cells were characterized and targets recognized by such antibodies were identified using immunoprecipitation and mass-spectrometry. One of the antibodies, AT1636, recognized a previously unidentified O-mannosylated 70kDa E-cadherin variant (ECV). Although the 70kDa ECV is found in all full-length E-cadherin expressing cells, tumor-specific binding of AT1636 is dependent on the O-mannosylation pattern in the antibody epitope on ECV. Using shRNA knock-down AT1636 binding was shown to depend on the transmembrane O-mannosyltransferase targeting cadherins 3 (TMTC3). 3 In accordance, coexpression of TMTC3 and E-cadherin in tumor cells is predictive for AT1636 binding. In addition, we observed that (over)expression of ECV results in a strong de-adhesive, EMT-like phenotype. Although AT1636 by itself is not able to induce ADCC, the CD3-bispecific antibody (single-chain UCHT1) AT1636 format specifically killed CRC cell lines.ConclusionsThe AT1636 antibody retrieved from a patient with Lynch syndrome binds a previous unidentified cancer-specific O-mannosylated 70kDa form of E-cadherin. This variant might play a role in tumor-cell invasion and metastasis. More importantly, we provide a rationale to advance AT1636 based therapeutics for treatment of CRC.Ethics ApprovalThe study protocol was approved by the Medical Ethical Committee of the Academic Medical Centre, Amsterdam, The Netherlands (NL42718.018.12)ReferencesM.J. Kwakkenbos, et al. Generation of stable monoclonal antibody-producing B cell receptor-positive human memory B cells by genetic programming. Nature Medicine 2010;16:123–128.K. Wagner, et al. Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity. Proc Natl Acad Sci USA 2014; 111: 16820–16825.I.S.B. Larsen, et al. Discovery of an O-mannosylation pathway selectively serving cadherins and protocadherins. Proc Natl Acad Sci USA 2017;114:11163–11168.
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Ristuccia, Roslyn, Xavier C. Badoux, Melinda Gibson, Admir Huseincehajic, and Judith Trotman. "Increasing Cross-Referral and Recruitment to Clinical Trials: A New Approach." Blood 126, no. 23 (December 3, 2015): 636. http://dx.doi.org/10.1182/blood.v126.23.636.636.
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Abstract Background/Aims The Haematology Clinical Research Network of New South Wales & the Australian Capital Territory (HCRN NSW/ACT) comprises public hospital clinical trial unit managers committed to collaboration in the clinical research endeavour. In June 2013 we launched the ClinTrial Refer Application (App) on iTunes and Google play. This smart-phone and iPAD/Tablet tool provided clinicians, research staff and patients with instant knowledge of currently recruiting local haematology trials. It was associated with an immediate increase in inter-hospital cross referrals of patients to trials. Following this, the HCRN aimed to sustain and measure this increased referral and trials recruitment and to create a not-for-profit 'template App' that could be transferrable to other clinical trial portfolios. Methods Patient cross-referral patterns, recruitment and staffing data were obtained from each of the 18 contributing hospitals in the HCRN from June 2013 to June 2015 and compared with prior to June 2013. Google analytics for the App were downloaded. We worked with research staff of other cancer networks to create modified versions of the App, establishing search functions unique to each network's geography and/or tumour stream. Newly derived Apps had to conform to the specifications of ClinTrial Refer, namely being publically available and free to download, simple to use and hosting only publically listed data of currently recruiting trials. Results Within the HCRN there has been a sustained increase in cross referrals for clinical trials (median 1/month (range 0-6) to 9/month (0-18)) (Figure 1) and a state-wide >50% increase in recruitment from 300 to 460 from 2012 to 2014 and a 20% increase in unit staffing from 2013 to 2014. Google analytics usage metrics identify 3362 App users, over 24,703 sessions lasting an average 56 seconds. 19,907 of these sessions are in NSW where 91% of users are repeat users. Ten other state and national haematology or other tumour stream ClinTrial Refer Apps have been derived from the original App, from Sept 2013 to July 2015 (Figure 2). While maintaining the basic structure of an easy-to-navigate listing of currently recruiting trials, each new App has been re-designed to ensure relevance for the needs of each network. This ranged from a simple re-configuration of the logo, splash screen and recruiting locations for other Australian haematology Apps, to providing mutational status options for melanoma trials or age criteria for paediatric and adolescent- young adult Networks. The back-end database of listed trials, selection criteria and recruiting sites can be rapidly easily updated by local trial unit managers ensuring currency of trial information (est. 30 minutes/month in total). The "early adopters" within other cancer research networks have reported a similar upswing in trials recruitment and Google analytics for these newer Apps are similarly impressive. Recognising ClinTrial Refer as an effective tool for patients to identify recruiting trials close to home, cancer consumer groups have posted the Apps on their websites. Conclusions An instantly accessible, simple smartphone Application has provided better knowledge management of local clinical trials across the spectrum of rare haematologic malignancies. A tool to facilitate collaboration in clinical research, it has significantly enhanced cross-referral and recruitment rates, increasing patient access to emerging therapies and supporting the viability of haematology clinical trial units across Australia. ClinTrial Refer has been rapidly adapted to suit the trials portfolios of other clinical trial networks, both within and beyond cancer. Figure 1. Figure 1. Figure 2. Figure 2. Disclosures No relevant conflicts of interest to declare.
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Abegaz, Silamlak Birhanu. "Human ABO Blood Groups and Their Associations with Different Diseases." BioMed Research International 2021 (January 23, 2021): 1–9. http://dx.doi.org/10.1155/2021/6629060.
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Introduction. Human ABO blood type antigens exhibit alternative phenotypes and genetically derived glycoconjugate structures that are located on the red cell surface which play an active role in the cells’ physiology and pathology. Associations between the blood type and disease have been studied since the early 1900s when researchers determined that antibodies and antigens are inherited. However, due to lack of antigens of some blood groups, there have been some contentious issues with the association between the ABO blood group and vulnerability to certain infectious and noninfectious diseases. Objective. To review different literatures that show the association between ABO blood groups and different diseases. Method. Original, adequate, and recent articles on the same field were researched, and the researcher conducted a comprehensive review on this topic. Thus, taking out critical discussions, not only a descriptive summary of the topic but also contradictory ideas were fully retrieved and presented in a clear impression. In addition, some relevant scientific papers published in previous years were included. The article search was performed by matching the terms blood types/groups with a group of terms related to different diseases. The articles were screened and selected based on the title and abstract presented. Results. The susceptibility to various diseases, such as cancer, cardiovascular diseases, infections and hematologic disorders, cognitive disorders, circulatory diseases, metabolic diseases, and malaria, has been linked with ABO blood groups. Moreover, blood group AB individuals were found to be susceptible to an increased risk of cognitive impairment which was independent of geographic region, age, race, and gender. Disorders such as hypertension, obesity, dyslipidemia, cardiovascular disease (CVD), and diabetes were also more prevalent in individuals with cognitive impairment. Early etiological studies indicated that blood type O has a connection with increased incidence of cholera, plague, tuberculosis infections, and mumps, whereas blood type A is linked with increased incidence of smallpox and Pseudomonas aeruginosa infection; blood type B is also associated with increased incidence of gonorrhea, tuberculosis, and Streptococcus pneumoniae, E. coli, and salmonella infections; and blood type AB is associated with increased incidence of smallpox and E. coli and salmonella infections. Diabetes mellitus, hypercholesterolemia, arterial hypertension, and family history for ischemic heart disease are the most common risk factors for cardiovascular diseases and can be genetically transmitted to offspring. Higher incidence of cancers in the stomach, ovaries, salivary glands, cervix, uterus, and colon/rectum was common in blood type A people than in O type people. The link between the ABO blood type and thromboembolic diseases and bleeding risk are intervened by the glycosyltransferase activity and plasma levels and biologic activity of vWF (Von Willebrand factor), a carrier protein for coagulation factor VIII which is low in O type. Conclusion. Several studies related to the ABO phenotype show that genetically determined human ABO blood groups were correspondingly linked with an increased risk of various infectious and noninfectious diseases. However, further investigations are needed particularly on the molecular level of ABO blood groups and their association with various diseases.
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Sanna, Antonio. "Adapting The Tempest." Kinema: A Journal for Film and Audiovisual Media, April 15, 2015. http://dx.doi.org/10.15353/kinema.vi.1321.
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ADAPTING THE TEMPEST: JULIE TAYMOR'S REVELS Adaptations and appropriations of Shakespeare's plays demonstrate the unending life of a series of canonical texts that still invite numerous forms of interpretation. Simultaneously, they also offer a tool for the (re)interpretation of the English playwright's works. This is definitely the case of Julie Taymor's recent film The Tempest (2010), which partially alters the structure of Shakespeare's last solo play, but also actively engages with the academic debates on the work's thematic concerns. Nevertheless, Taymor's film could be seen as faithful to the original source and as fully realizing on the screen its fantastic elements, especially through the use of the special effects. Taymor's The Tempest utilizes the original heightened language of the seventeenth century - which certainly gratifies those spectators that enjoy what Stephen Greenblatt has defined as Shakespeare's "infinite delight in language"(1) - and maintains the main dialogues of the original...
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De Bonis, Giuseppe. "Alfred Hitchcock presents: Multilingualism as a vehicle for… suspense. The Italian dubbing of Hitchcock’s multilingual films." Linguistica Antverpiensia, New Series – Themes in Translation Studies, no. 13 (May 28, 2013). http://dx.doi.org/10.52034/lanstts.v0i13.46.
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This study aims to shed light on the role multilingualism plays in Alfred Hitchcock’s films, by examining the different solutions adopted by Italian audiovisual translators to cope with the multilingual situations represented. The comparative analysis carried out between the original versions of the thirteen multilingual films directed by Hitchcock and their Italian dubbed versions has identified three different macro-strategies: first of all, the neutralisation of the different languages present in the original version via dubbing the entire film into Italian. Secondly, the preservation of the different lingua-cultural identities through a combination of dubbing with other screen translation modalities such as contextual translation, and interpretation performed by one the film’s characters. Finally, a third strategy is the quantitative reduction of the multilingual situations present in the original version of the film, with all the inevitable consequences that this may have on viewers’ suspension of linguistic disbelief.