Academic literature on the topic 'Ouabain'

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Journal articles on the topic "Ouabain"

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Fu, Yong, Dalian Ding, Lei Wei, Haiyan Jiang, and Richard Salvi. "Ouabain-Induced Apoptosis in Cochlear Hair Cells and Spiral Ganglion NeuronsIn Vitro." BioMed Research International 2013 (2013): 1–16. http://dx.doi.org/10.1155/2013/628064.

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Ouabain is a common tool to explore the pathophysiological changes in adult mammalian cochleain vivo. In prior studies, locally administering ouabain via round window membrane demonstrated that the ototoxic effects of ouabainin vivovaried among mammalian species. Little is known about the ototoxic effectsin vitro. Thus, we prepared cochlear organotypic cultures from postnatal day-3 rats and treated these cultures with ouabain at 50, 500, and 1000 μM for different time to elucidate the ototoxic effects of ouabainin vitroand to provide insights that could explain the comparative ototoxic effects
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Teixeira, Mariana Pires, Natalia Ferreira Haddad, Eliza Freitas Passos, et al. "Ouabain Effects on Human Anaplastic Thyroid Carcinoma 8505C Cells." Cancers 14, no. 24 (2022): 6168. http://dx.doi.org/10.3390/cancers14246168.

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Anaplastic thyroid carcinoma (ATC) is a rare, but aggressive, carcinoma derived from follicular cells. While conventional treatments may improve patients’ survival, the lethality remains high. Therefore, there is an urgent need for more effective ATC treatments. Cardiotonic steroids, such as ouabain, have been shown to have therapeutic potential in cancer treatment. Thus, we aimed to evaluate ouabain’s effects in human anaplastic thyroid cells. For this, 8505C cells were cultured in the presence or absence of ouabain. Viability, cell death, cell cycle, colony formation and migratory ability we
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Curras, M. C., and J. A. Boulant. "Effects of ouabain on neuronal thermosensitivity in hypothalamic tissue slices." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 257, no. 1 (1989): R21—R28. http://dx.doi.org/10.1152/ajpregu.1989.257.1.r21.

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To determine the role of the electrogenic Na+-K+ pump in neuronal thermosensitivity, single-unit activity was recorded in rat hypothalamic tissue slices before, during, and after perfusions containing 10(-5) or 10(-6) M ouabain, a specific pump inhibitor. Most neurons were recorded in the preoptic-anterior hypothalamus. Some neurons were also tested with high magnesium-low calcium perfusions to determine ouabain's effects on neuronal activity during synaptic blockade. When the neurons were characterized according to thermosensitivity, 24% were warm sensitive, 8% were cold sensitive, and 68% we
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Blanco, Gustavo, and Darren P. Wallace. "Novel role of ouabain as a cystogenic factor in autosomal dominant polycystic kidney disease." American Journal of Physiology-Renal Physiology 305, no. 6 (2013): F797—F812. http://dx.doi.org/10.1152/ajprenal.00248.2013.

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The classic role of the Na-K-ATPase is that of a primary active transporter that utilizes cell energy to establish and maintain transmembrane Na+ and K+ gradients to preserve cell osmotic stability, support cell excitability, and drive secondary active transport. Recent studies have revealed that Na-K-ATPase located within cholesterol-containing lipid rafts serves as a receptor for cardiotonic steroids, including ouabain. Traditionally, ouabain was viewed as a toxin produced only in plants, and it was used in relatively high concentrations to experimentally block the pumping action of the Na-K
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Lewis, Lynley K., Timothy G. Yandle, Philip J. Hilton, Berit P. Jensen, Evan J. Begg, and M. Gary Nicholls. "Endogenous Ouabain Is Not Ouabain." Hypertension 64, no. 4 (2014): 680–83. http://dx.doi.org/10.1161/hypertensionaha.114.03919.

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Sibarov, Dmitry A., Zoia D. Zhuravleva, Margarita A. Ilina, et al. "Unveiling the Role of Cholesterol in Subnanomolar Ouabain Rescue of Cortical Neurons from Calcium Overload Caused by Excitotoxic Insults." Cells 12, no. 15 (2023): 2011. http://dx.doi.org/10.3390/cells12152011.

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Na/K-ATPase maintains transmembrane ionic gradients and acts as a signal transducer when bound to endogenous cardiotonic steroids. At subnanomolar concentrations, ouabain induces neuroprotection against calcium overload and apoptosis of neurons during excitotoxic stress. Here, the role of lipid rafts in interactions between Na/K-ATPase, sodium–calcium exchanger (NCX), and N-methy-D-aspartate receptors (NMDARs) was investigated. We analyzed 0.5–1-nanometer ouabain’s effects on calcium responses and miniature post-synaptic current (mEPSCs) frequencies of cortical neurons during the action of NMD
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Gomez-Sanchez, Elise P., Mark F. Foecking, Deborah Sellers, Mary S. Blankenship, and Celso E. Gomez-Sanchez. "Is the Circulating Ouabain-like Compound Ouabain?" American Journal of Hypertension 7, no. 7_Pt_1 (1994): 637–46. http://dx.doi.org/10.1093/ajh/7.7.637.

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Gomez-Sanchez, Elise P., Mark F. Foecking, Sellers Deborah, Mary S. Blankenship, and Celso E. Gomez-Sanchez. "Is the Circulating Ouabain-like Compound Ouabain?" American Journal of Hypertension 7, no. 7_Pt_1 (1994): 647–50. http://dx.doi.org/10.1093/ajh/7.7.647.

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Grajpel, Barbara, Przemysław Grodzicki, and Leszek Janiszewski. "The effect of hypoxia and ouabain on the muscle resting potential of Periplaneta americana L." Acta Neurobiologiae Experimentalis 56, no. 1 (1996): 103–6. http://dx.doi.org/10.55782/ane-1996-1109.

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We have studied the effect of hypoxia and ouabain on the muscle resting potential (RP) of the cockroach Periplaneta americana L. The experiments were done in insects reared either in normoxic or hypoxic conditions and treated, or not treated, with ouabain. Hypoxic conditions decreased the RP by about. 11.6 mV. Ouabain decreased the RP by about 8.3 mV (ouabain 10(-5) mol/l) or 12.3 mV (ouabain 5 x 10(-5) mol/l). Hypoxia and ouabain decreased the RP by about 28.5 mV (ouabain 10(-5) mol/l) or 31.3 mV (ouabain 5 x 10(-5) mol/l).
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Kjeldsen, K. "Homogeneity of [3H]ouabain-binding sites in rat soleus muscle." Biochemical Journal 249, no. 2 (1988): 481–85. http://dx.doi.org/10.1042/bj2490481.

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Homogeneity or heterogeneity of rat soleus-muscle Na,K-ATPase (Na+ + K+-dependent ATPase) with respect to affinity for [3H]ouabain was evaluated. Since the standard method for measuring specific [3H]ouabain binding to rat skeletal-muscle samples includes subtraction of a value for non-specific [3H]ouabain uptake and retention, and a wash-out in the cold to remove [3H]ouabain from the extracellular phase, it was possible that these procedures could hide a class of [3H]ouabain-binding sites either with low affinity or with a rapid dissociation of [3H]ouabain. However, measurements of [3H]ouabain
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Dissertations / Theses on the topic "Ouabain"

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Cellnik, Torsten [Verfasser]. "Defunktionalisierung von Ouabain / Torsten Cellnik." Wuppertal : Universitätsbibliothek Wuppertal, 2019. http://d-nb.info/118842209X/34.

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Magnusson, Emma. "Ouabain Toxicity -Selectivity Towards Renal Cancer Cells." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278574.

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Ouabain and other cardiotonic steroids are known to inhibit Na + ,K + -ATPase (NKA), theion pump responsible for the ionic gradient across the plasma membrane. These steroidsdisplay a selective toxicity towards several tumour cells in comparison to primary humancells, however, the mechanism behind this is not yet understood. Here, we examined theouabain toxicity in renal epithelial cells, proximal tubular cells (PTCs) of different origin. Weinvestigated the relative cytotoxicity in cancer cells (A-498) and papilloma virus-transformedPTCs (HK-2) as well as to primary human PTCs (hPTC) to valida
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Harwood, Steven Michael. "Development and application of an immunoassay for ouabain and a study of the nature of endogenous ouabain-like compound." Thesis, Queen Mary, University of London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325540.

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Carneiro, Luciana Teles. "Efeito modulador da ouabaína no sistema imunológico." Universidade Federal da Paraí­ba, 2011. http://tede.biblioteca.ufpb.br:8080/handle/tede/6866.

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Made available in DSpace on 2015-05-14T13:00:17Z (GMT). No. of bitstreams: 1 parte1.pdf: 1834339 bytes, checksum: 9a90c51efd47d1b0c436c6b761659ab0 (MD5) Previous issue date: 2011-02-21<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>Initially known as a cardiotonic steroid capable to inhibit the Na+/K+ATPase, ouabain was identified as an endogenous substance present in human plasma, produced by the adrenal, pituitary and hypothalamus and can interfere with various aspects of immune response. In this study, which aimed to study the modulating effect of ouabain
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Tennant, Brian Prichard. "Biosynthesis and physiological characteristics of endogenous ouabain-like substance." Thesis, King's College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272367.

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Semra, Yemane Kurban. "Endogenous ouabain-like immunoreactive substance (OLIS) : characterisation and physiological studies." Thesis, King's College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313282.

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Vasconcelos, Danielle Ingrid Bezerra de. "Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção." Universidade Federal da Paraí­ba, 2011. http://tede.biblioteca.ufpb.br:8080/handle/tede/3640.

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Made available in DSpace on 2015-04-01T14:16:00Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 5574506 bytes, checksum: a2d785f5339e8cb5e573693458ab5abd (MD5) Previous issue date: 2011-09-30<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>Ouabain, known as a cardiotonic glycoside capable of inhibiting the Na+/K+ ATPase, was widely used for heart failure treatment. Identified as an endogenous substance, ouabain is capable of interfering with various physiological functions, including immune system modulation. Besides that, little is known about the involvement o
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Gillingwater, Scott David. "Purification and characterisation of ouabain-like compound(s) from biological material." Thesis, King's College London (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418070.

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Harris, Tanoya L. "Ouabain Regulates Caveolin-1 Vesicle Trafficking by a Src-Dependent Mechanism." University of Toledo Health Science Campus / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=mco1333732028.

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Trenti, Annalisa. "Analysis of the molecular mechanisms of the antineoplastic effect of ouabain." Doctoral thesis, Università degli studi di Padova, 2012. http://hdl.handle.net/11577/3422167.

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Ouabain is a cardiac glycoside whose primary action is inhibition of Na/K ATPase activity, a ubiquitous enzyme responsible for translocating Na and K ions across the cell membrane using ATP as the driving force. It has been demonstrated that the Na/K ATPase also functions as a classical receptor, capable of converting cardiac glycodise binding into activation of various protein kinase cascades (Liu and Xie, 2010). Also, recent studies have shown that cardiac glycosides selectively inhibit cell proliferation and/or induce apoptosis in several cancer cell lines (Schoner and Sheiner-Bobis, 2007).
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Books on the topic "Ouabain"

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Ludwig, Karin. Bindungsstudien mit -Ouabain an intakten menschlichen mononukleären Leukozyten. [s.n.], 1985.

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Dare, Amos O. Ouabain potentiates kainate neurotoxicity: A new rat model of human temporal lobe epilepsy. s.n.], 1996.

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Feinauer, Marianne. Der Einfluss von Ouabain auf die Acetylcholinfreisetzung. 1988.

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Jeck, Dörte. Charakterisierung der Wirkung von Ouabain auf die Freisetzung von Acetylcholin im isolierten Herzen. 1986.

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Kjeldsen, Keld. Regulation of the concentration of 3H-Ouabain binding sites in mammalian skeletal muscle: Effects of age, K-depletion thyroid status and hypertension. 1986.

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Morton, Sarah Wentworth. Ouabi; Or The Virtues Of Nature: An Indian Tale In Four Cantos. Kessinger Publishing, LLC, 2007.

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Morton, Sarah Wentworth. Ouabi; Or The Virtues Of Nature: An Indian Tale In Four Cantos. Kessinger Publishing, LLC, 2007.

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Book chapters on the topic "Ouabain"

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Ezzaher, Abdellatif, Renaud Mougenot, Alain Gerbi, et al. "Non Effectiveness of Ouabain and Decrease in Na+/K+ -ATPase Affinity for Ouabain in Failing Rabbit Heart." In The Sodium Pump. Steinkopff, 1994. http://dx.doi.org/10.1007/978-3-642-72511-1_151.

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Hamlyn, J. M. "Discovery of Endogenous Ouabain — A New Mammalian Hormone." In The Sodium Pump. Steinkopff, 1994. http://dx.doi.org/10.1007/978-3-642-72511-1_132.

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Navaratnam, S., and J. C. Khatter. "Protective Effect of Nifedipine upon Ouabain-Induced Arrhythmias." In Developments in Cardiovascular Medicine. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-2057-9_5.

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Lingrel, Jerry B., James Van Huysse, William O’Brien, Elizabeth Jewell-Motz, and Patrick Schultheis. "Amino Acid Residues Involved in Ouabain Sensitivity and Cation Binding." In Molecular and Cellular Mechanisms of H+ Transport. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-79301-1_20.

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Lee, Yee-Ki, Kwong-Man Ng, Wing-Hon Lai, et al. "Differentiation of Embryonic Stem Cells into Cardiomyocytes: Role of Ouabain." In Stem Cells and Cancer Stem Cells, Volume 6. Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-2993-3_7.

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Rhee, Hee Min. "Parallelism Between Transport Inhibition and L1210 Cell Growth by Ouabain." In Oxygen Transport to Tissue X. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4615-9510-6_89.

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Stokke, E. S., J. Østensen, and F. Kiil. "Effect of Ouabain and Acetazolamide on Proximal Tubular Transport in Dogs." In Diuretics: Basic, Pharmacological, and Clinical Aspects. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-2067-8_17.

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Grupp, G., Ingrid L. Grupp, T. Hickerson, S. W. Lee, and A. Schwartz. "Biphasic contractile response to ouabain: Species specific? Calcium dependent? Altered sensitivity?" In Cardiac Glycosides 1785–1985. Steinkopff, 1986. http://dx.doi.org/10.1007/978-3-662-11292-2_15.

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Petzinger, E., K. Fischer, and H. Fasold. "Role of the bile acid transport system in hepatocellular ouabain uptake." In Cardiac Glycosides 1785–1985. Steinkopff, 1986. http://dx.doi.org/10.1007/978-3-662-11292-2_40.

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de Lurdes Gonçalves, Maria. "Ouabain binding to erythrocytes from neonates, blood cord and pregnant women." In Cardiac Glycosides 1785–1985. Steinkopff, 1986. http://dx.doi.org/10.1007/978-3-662-11292-2_46.

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Conference papers on the topic "Ouabain"

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Krivoi, Igor. "ENDOGENOUS OUABAIN AND SKELETAL MUSCLE." In XV International interdisciplinary congress "Neuroscience for Medicine and Psychology". LLC MAKS Press, 2019. http://dx.doi.org/10.29003/m446.sudak.ns2019-15/245-246.

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Deryagina, E. V., and K. A. Makarova. "EFFECT OF SODIUM DEOXYCHOLATE AND TRITON X-100 ON THE ACTIVITY OF NA,K-ATPASE OF THE MIXED MEMBRANE FRACTION OF THE CEREBRAL CORTEX OF RATS." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-313.

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Na+/K+-ATPase is an asymmetrically organized protein that maintains membrane potential. The active centers are intracellular; detection of activity without an increase in membrane permeability is not possible. The aim of the study was to study the activity of ouabain-sensitive and ouabain-resistant isoforms in the mixed membrane fraction of the cerebral cortex of rats exposed to the detergents Triton X-100 and Dox-Na.
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Bikmurzina, Anastasia, Arina Fedorova, Igor Krivoi, and Alexander Markov. "CHANGES IN THE BLOOD-BRAIN BARRIER OF RATS WITH CHRONIC INJECTIONS OF OUABAIN." In XVI International interdisciplinary congress "Neuroscience for Medicine and Psychology". LLC MAKS Press, 2020. http://dx.doi.org/10.29003/m951.sudak.ns2020-16/100.

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Wu, Jun, Mariele Mondala, Meng-Yin Hsieh, Eugene Roberts, and Richard Ermel. "Abstract 4403: Antitumor properties of ouabain in lipid double emulsion integrated with tumor cell membrane fractions." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-4403.

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Rhee, Chung-Ku, Sung Huyn Bae, So-Young Chang, Phil-Sang Chung, and Jae-Yun Jung. "Effect of low level laser therapy (LLLT) on ouabain induced auditory neuropathy in gerbils (Conference Presentation)." In Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology, edited by Brian J. F. Wong and Justus F. Ilgner. SPIE, 2016. http://dx.doi.org/10.1117/12.2212553.

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Kravtsova, Violetta, Daria Ganke, Maria Tishkova, et al. "HYPOBARIC HYPOXIA MODULATES LEVEL OF CIRCULATING OUABAIN AND Na,K-ATPase FUNCTIONING IN RAT DIAPHRAGM MUSCLE." In XIX INTERNATIONAL INTERDISCIPLINARY CONGRESS NEUROSCIENCE FOR MEDICINE AND PSYCHOLOGY. LCC MAKS Press, 2023. http://dx.doi.org/10.29003/m3273.sudak.ns2023-19/159-160.

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Wu, Jun, Meng-Yin Hsieh, Yan Wang, et al. "Abstract 5412: Antitumor properties of ouabain in lipid double emulsion in orthotopic canine osteosarcoma xenografted mouse model." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-5412.

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Hwang, Mihwa, Dong Wha Jun, Hyun Jung Kim, Soo Kyung Hwang, Chang-Hun Lee, and Sunshin Kim. "Abstract 3335: Ouabain, a cardiac glycoside, inhibits the Fanconi Anemia-BRCA pathway activated by DNA crosslinking agents." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3335.

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Furihata, Kenichi, Diane J. Nugent, Amy L. Bissonette, Elizabeth Vokac, and Thomas J. Kunicki. "PRODUCTION OF HUMAN MONOCLONAL ANTIBODIESSPECIFIC FOR PLATELET MEMBRANE GLYCOPROTEIN IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643705.

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Human monoclonal antibodies specific for platelet membrane glycoproteins (GPs) arepotentially important reagentsfor studies of the immunogenicity of membrane glycoproteins. A human monoclonalautoantibody, 5E5, reactive with plateletGPIIIa has been developed (Nugent, et al.,Blood, 1987, in press). In this report, we describe the production of additional human monoclonal antibodies specific for GPIIIa. Peripheral blood lymphocytes fromone patient with post-transfusion purpur(PTP) and one woman who had delivered an infant with neonatal alloimmune thrombocytopenic purpura (NATP) were used as a sou
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