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1

Ashmore, Jonathan. "Outer Hair Cells and Electromotility." Cold Spring Harbor Perspectives in Medicine 9, no. 7 (2018): a033522. http://dx.doi.org/10.1101/cshperspect.a033522.

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2

Ashmore, Jonathan. "Cochlear Outer Hair Cell Motility." Physiological Reviews 88, no. 1 (2008): 173–210. http://dx.doi.org/10.1152/physrev.00044.2006.

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Normal hearing depends on sound amplification within the mammalian cochlea. The amplification, without which the auditory system is effectively deaf, can be traced to the correct functioning of a group of motile sensory hair cells, the outer hair cells of the cochlea. Acting like motor cells, outer hair cells produce forces that are driven by graded changes in membrane potential. The forces depend on the presence of a motor protein in the lateral membrane of the cells. This protein, known as prestin, is a member of a transporter superfamily SLC26. The functional and structural properties of pr
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3

Gummer, Anthony W., Jens Meyer, Gerhard Frank, Marc P. Scherer, and Serena Preyer. "Mechanical Transduction in Outer Hair Cells." Audiology and Neurotology 7, no. 1 (2002): 13–16. http://dx.doi.org/10.1159/000046856.

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4

Zenner, Hans Peter. "Motile responses in outer hair cells." Hearing Research 22, no. 1-3 (1986): 83–90. http://dx.doi.org/10.1016/0378-5955(86)90082-1.

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5

Prieto, J., J. A. Merchán, P. Gil-Loyzaga, and J. Rueda. "Subsurface material in outer hair cells." Hearing Research 21, no. 3 (1986): 277–80. http://dx.doi.org/10.1016/0378-5955(86)90225-x.

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6

Stauffer, Eric A., and Jeffrey R. Holt. "Sensory Transduction and Adaptation in Inner and Outer Hair Cells of the Mouse Auditory System." Journal of Neurophysiology 98, no. 6 (2007): 3360–69. http://dx.doi.org/10.1152/jn.00914.2007.

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Auditory function in the mammalian inner ear is optimized by collaboration of two classes of sensory cells known as inner and outer hair cells. Outer hair cells amplify and tune sound stimuli that are transduced and transmitted by inner hair cells. Although they subserve distinct functions, they share a number of common properties. Here we compare the properties of mechanotransduction and adaptation recorded from inner and outer hair cells of the postnatal mouse cochlea. Rapid outer hair bundle deflections of about 0.5 micron evoked average maximal transduction currents of about 325 pA, wherea
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7

Jia, Shuping, and David Z. Z. He. "Motility-associated hair-bundle motion in mammalian outer hair cells." Nature Neuroscience 8, no. 8 (2005): 1028–34. http://dx.doi.org/10.1038/nn1509.

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8

Biswas, Joyshree, Robert S. Pijewski, Rohit Makol, et al. "C1ql1 is expressed in adult outer hair cells of the cochlea in a tonotopic gradient." PLOS ONE 16, no. 5 (2021): e0251412. http://dx.doi.org/10.1371/journal.pone.0251412.

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Hearing depends on the transduction of sounds into neural signals by the inner hair cells of the cochlea. Cochleae also have outer hair cells with unique electromotile properties that increase auditory sensitivity, but they are particularly susceptible to damage by intense noise exposure, ototoxic drugs, and aging. Although the outer hair cells have synapses on afferent neurons that project to the brain, the function of this neuronal circuit is unclear. Here, we created a novel mouse allele that inserts a fluorescent reporter at the C1ql1 locus which revealed gene expression in the outer hair
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9

Chertoff, M. E., and W. E. Brownell. "Characterization of cochlear outer hair cell turgor." American Journal of Physiology-Cell Physiology 266, no. 2 (1994): C467—C479. http://dx.doi.org/10.1152/ajpcell.1994.266.2.c467.

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The cochlear outer hair cell (OHC) is a cylindrical cell with structural features suggestive of a hydraulic skeleton, i.e., an elastic shell with a positive internal pressure. This study characterizes the role of the OHC elevated cytoplasmic pressure in maintaining the cell shape. Intracellular pressure of OHCs from guinea pig is estimated by measuring changes in cell morphology in response to increasing or decreasing osmolarity. Cells collapse when subjected to a continuous increase in osmolarity. Collapse occurs at an average of 8 mosM above the standard medium, suggesting that normal cells
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10

Wada, Hiroshi. "Mechanics of inner and outer hair cells." AUDIOLOGY JAPAN 59, no. 3 (2016): 161–69. http://dx.doi.org/10.4295/audiology.59.161.

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11

Ashmore, Jonathan F. "Active cochlear mechanics and outer hair cells." Journal of the Acoustical Society of America 143, no. 3 (2018): 1809. http://dx.doi.org/10.1121/1.5035927.

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12

Wu, Tao, Sripriya Ramamoorthy, Teresa Wilson, et al. "Optogenetic Control of Mouse Outer Hair Cells." Biophysical Journal 110, no. 2 (2016): 493–502. http://dx.doi.org/10.1016/j.bpj.2015.11.3521.

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13

Dallos, Peter. "Cochlear amplification, outer hair cells and prestin." Current Opinion in Neurobiology 18, no. 4 (2008): 370–76. http://dx.doi.org/10.1016/j.conb.2008.08.016.

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14

Crist, Jennifer R., Maureen Fallon, and Richard P. Bobbin. "Volume regulation in cochlear outer hair cells." Hearing Research 69, no. 1-2 (1993): 194–98. http://dx.doi.org/10.1016/0378-5955(93)90107-c.

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15

Iwasa, Kuni H. "Kinetic Membrane Model of Outer Hair Cells." Biophysical Journal 120, no. 1 (2021): 122–32. http://dx.doi.org/10.1016/j.bpj.2020.11.017.

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16

Fu, Mingyu, Mengzi Chen, Xiao Yan, Xueying Yang, Jinfang Xiao, and Jie Tang. "The Effects of Urethane on Rat Outer Hair Cells." Neural Plasticity 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/3512098.

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The cochlea converts sound vibration into electrical impulses and amplifies the low-level sound signal. Urethane, a widely used anesthetic in animal research, has been shown to reduce the neural responses to auditory stimuli. However, the effects of urethane on cochlea, especially on the function of outer hair cells, remain largely unknown. In the present study, we compared the cochlear microphonic responses between awake and urethane-anesthetized rats. The results revealed that the amplitude of the cochlear microphonic was decreased by urethane, resulting in an increase in the threshold at al
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17

Cortese, Matteo, Samantha Papal, Francisco Pisciottano та ін. "Spectrin βV adaptive mutations and changes in subcellular location correlate with emergence of hair cell electromotility in mammalians". Proceedings of the National Academy of Sciences 114, № 8 (2017): 2054–59. http://dx.doi.org/10.1073/pnas.1618778114.

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The remarkable hearing capacities of mammals arise from various evolutionary innovations. These include the cochlear outer hair cells and their singular feature, somatic electromotility, i.e., the ability of their cylindrical cell body to shorten and elongate upon cell depolarization and hyperpolarization, respectively. To shed light on the processes underlying the emergence of electromotility, we focused on the βV giant spectrin, a major component of the outer hair cells' cortical cytoskeleton. We identified strong signatures of adaptive evolution at multiple sites along the spectrin-βV amino
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18

Knirsch, M., N. Brandt, C. Braig, et al. "Persistence of Cav1.3 Ca2+ Channels in Mature Outer Hair Cells Supports Outer Hair Cell Afferent Signaling." Journal of Neuroscience 27, no. 24 (2007): 6442–51. http://dx.doi.org/10.1523/jneurosci.5364-06.2007.

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19

Ramírez-Camacho, R., J. R. García-Berrocal, A. Trinidad, J. M. Verdaguer, and J. Nevado. "Blebs in inner and outer hair cells: a pathophysiological hypothesis." Journal of Laryngology & Otology 122, no. 11 (2008): 1151–55. http://dx.doi.org/10.1017/s002221510700134x.

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AbstractIntroduction:The ototoxic effects of cisplatin include loss of outer hair cells, degeneration of the stria vascularis and a decrease in the number of spiral ganglion cells. Scanning microscopy has shown balloon-like protrusions (blebs) of the plasma membrane of inner hair cells following cisplatin administration. The present study was undertaken to identify the possible role of inner and outer hair cell blebs in the pathogenesis of cisplatin-induced ototoxicity.Materials and methods:Twenty-five guinea pigs were injected with cisplatin and their hearing tested at different time-points,
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20

Schwartz, Ilsa, Chong-Sun Kim, and See-Ok Shin. "Ultrastructural Changes in the Cochlea of the Guinea Pig after Fast Neutron Irradiation." Otolaryngology–Head and Neck Surgery 110, no. 4 (1994): 419–27. http://dx.doi.org/10.1177/019459989411000412.

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Guinea pigs were irradiated with fast neutrons. After a single dose of 2, 6, 10, or 15 Gy was applied, scanning and transmission electron microscopy of the temporal bone was performed to assess the effect of fast neutron irradiation on the cochlea. Outer hair cell damage appeared with neutron irradiation of more than 10 Gy, and Inner hair cell damage with neutron Irradiation of more than 15 Gy. Outer hair cells were more severely damaged than Inner hair cells. No statistically significant differences were found in damage of basal, middle, and apical turns. The second and third rows of outer ha
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21

Meyer, Jens, Andreas F. Mack, and Anthony W. Gummer. "Pronounced infracuticular endocytosis in mammalian outer hair cells." Hearing Research 161, no. 1-2 (2001): 10–22. http://dx.doi.org/10.1016/s0378-5955(01)00338-0.

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22

Géléoc, Gwénaëlle S. G., and Jeffrey R. Holt. "Auditory amplification: outer hair cells pres the issue." Trends in Neurosciences 26, no. 3 (2003): 115–17. http://dx.doi.org/10.1016/s0166-2236(03)00030-4.

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23

Zheng, Jing, Laird D. Madison, Dominik Oliver, Bernd Fakler, and Peter Dallos. "Prestin, the Motor Protein of Outer Hair Cells." Audiology and Neurotology 7, no. 1 (2002): 9–12. http://dx.doi.org/10.1159/000046855.

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24

Magdolna Szõnyi, Péter Csermely, Is. "Acetylcholine-induced Phosphorylation in Isolated Outer Hair Cells." Acta Oto-Laryngologica 119, no. 2 (1999): 185–88. http://dx.doi.org/10.1080/00016489950181639.

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25

Kachar, Bechara, William E. Brownell, Richard Altschuler, and Jörgen Fex. "Electrokinetic shape changes of cochlear outer hair cells." Nature 322, no. 6077 (1986): 365–68. http://dx.doi.org/10.1038/322365a0.

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26

Holley, M. C., and J. F. Ashmore. "A cytoskeletal spring in cochlear outer hair cells." Nature 335, no. 6191 (1988): 635–37. http://dx.doi.org/10.1038/335635a0.

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27

Bohne, Barbara A., Gary W. Harding, and Steve C. Lee. "Death pathways in noise-damaged outer hair cells." Hearing Research 223, no. 1-2 (2007): 61–70. http://dx.doi.org/10.1016/j.heares.2006.10.004.

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28

Lu, Timothy K., Serhii Zhak, Peter Dallos, and Rahul Sarpeshkar. "Fast cochlear amplification with slow outer hair cells." Hearing Research 214, no. 1-2 (2006): 45–67. http://dx.doi.org/10.1016/j.heares.2006.01.018.

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29

Nadol, Joseph B., and Barbara J. Burgess. "Morphology of Synapses at the Base of Hair Cells in the Organ of Corti of the Chimpanzee." Annals of Otology, Rhinology & Laryngology 99, no. 3 (1990): 215–20. http://dx.doi.org/10.1177/000348949009900311.

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The synaptic morphology of inner and outer hair cells of the organ of Corti of the chimpanzee was evaluated by serial section electron microscopy. The morphology of nerve terminals and synapses at both sites was very similar to that of human and other mammalian species. Two types of nerve terminals, nonvesiculated and vesiculated, with distinct synaptic morphology were found. In addition, between some nonvesiculated endings and outer hair cells, a reciprocal synaptic relationship was seen. In such terminals there was morphologic evidence for transmission from hair cell to neuron and from neuro
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30

Khanna, Shyam M., Mats Ulfendahl, and Åke Flock. "Mechanical Tuning Characteristics of Outer Hair Cells and Hensen's Cells." Acta Oto-Laryngologica 108, sup467 (1989): 139–44. http://dx.doi.org/10.3109/00016488909138330.

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31

Murakoshi, Michio, and Hiroshi Wada. "GS1-30 SOUND AMPLIFICATION MECHANISM BY THREE ROWS OF OUTER HAIR CELLS IN MAMMALS(GS1: Cell and Tissue Biomechanics VI)." Proceedings of the Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics 2015.8 (2015): 141. http://dx.doi.org/10.1299/jsmeapbio.2015.8.141.

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32

Witt, C. M., H. Y. Hu, W. E. Brownell, and D. Bertrand. "Physiologically silent sodium channels in mammalian outer hair cells." Journal of Neurophysiology 72, no. 2 (1994): 1037–40. http://dx.doi.org/10.1152/jn.1994.72.2.1037.

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1. Voltage-dependent properties of isolated guinea pig outer hair cells (OHCs) were investigated using whole-cell recording. An inward current was detected in approximately 10% of the cells. This inward current was identified as belonging to the voltage-activated sodium current family on the basis of its high sensitivity to tetrodotoxin and the effect of substitution of impermeant ions. Although this is the first report of a sodium current in the mammalian cochlea, it differs from the classical neuronal sodium current by having a variable magnitude from cell to cell and an inactivation that is
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33

Yi, Li, and He David Z. "The Cochlear Amplifier: Is it Hair Bundle Motion of Outer Hair Cells?" Journal of Otology 9, no. 2 (2014): 64–72. http://dx.doi.org/10.1016/s1672-2930(14)50017-7.

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34

Ohnishi, S., M. Hara, M. Inoue, et al. "Delayed shortening and shrinkage of cochlear outer hair cells." American Journal of Physiology-Cell Physiology 263, no. 5 (1992): C1088—C1095. http://dx.doi.org/10.1152/ajpcell.1992.263.5.c1088.

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Slow shortening of cochlear outer hair cells has been speculated to modify cochlear sensitivity. Tetanic electrical field stimulation of isolated outer hair cells from guinea pigs shortened the cells for 2-3 min. Electrical stimulation reduced cell length and volume (-13.5 +/- 1.5 and -37.3 +/- 3.0% of initial values, respectively, n = 16) and decreased the intracellular Cl- concentration. Cytochalasin B (100 microM) inhibited electrical stimulation-induced shortening but not volume reduction. The following chemicals or manipulations inhibited the responses: 10 microM furosemide, 0.1 mM 4,4'-d
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35

Chole, Richard A., and Maggie Chiu. "Cochlear Hair Cell Stereocilia Loss in LP/J Mice with Bone Dysplasia of the Middle Ear." Annals of Otology, Rhinology & Laryngology 98, no. 6 (1989): 461–65. http://dx.doi.org/10.1177/000348948909800613.

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LP/J inbred mice spontaneously develop bony lesions of the middle ear and otic capsule that are similar to those of human otosclerosis and tympanosclerosis. These mice also have progressive loss of hearing due to cochlear hair cell loss. The purpose of this study was to describe quantitatively the deterioration and loss of cochlear hair cells to serve as a basis for future experiments attempting to alter the course of this disorder. Cochleas from 37 LP/J inbred mice were examined by scanning electron microscopy. The stereocilia loss in the cochlea was evident as early as 15 weeks of age and pr
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36

Roberts, DG. "Root-hair structure and development in the seagrass Halophila ovalis (R. Br.) Hook. F." Marine and Freshwater Research 44, no. 1 (1993): 85. http://dx.doi.org/10.1071/mf9930085.

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The seagrass Halophila ovalis normally produces one mature root, covered with a permanent mat of root hairs, per node. In this study, the development of the root hairs increased the effective root surface absorptive area by 215%. Of the root surface examined, 39% was devoted to root-hair production. Epidermal cells that produced root hairs contained more cytoplasm, endoplasmic reticulum and Golgi bodies than did adjacent hairless cells. In addition to appearing to be more metabolically active, root-hair-producing cells had a greater number of plasmodesmatal connections with the underlying oute
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37

Wiwatpanit, Teerawat, Sarah M. Lorenzen, Jorge A. Cantú, et al. "Trans-differentiation of outer hair cells into inner hair cells in the absence of INSM1." Nature 563, no. 7733 (2018): 691–95. http://dx.doi.org/10.1038/s41586-018-0570-8.

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38

Drexl, Markus, Marcia M. Mellado Lagarde, Jian Zuo, Andrei N. Lukashkin, and Ian J. Russell. "The Role of Prestin in the Generation of Electrically Evoked Otoacoustic Emissions in Mice." Journal of Neurophysiology 99, no. 4 (2008): 1607–15. http://dx.doi.org/10.1152/jn.01216.2007.

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Electrically evoked otoacoustic emissions are sounds emitted from the inner ear when alternating current is injected into the cochlea. Their temporal structure consists of short- and long-delay components and they have been attributed to the motile responses of the sensory-motor outer hair cells of the cochlea. The nature of these motile responses is unresolved and may depend on either somatic motility, hair bundle motility, or both. The short-delay component persists after almost complete elimination of outer hair cells. Outer hair cells are thus not the sole generators of electrically evoked
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39

Nadol, Joseph B., and Aaron R. Thornton. "Ultrastructural Findings in a Case of Meniere's Disease." Annals of Otology, Rhinology & Laryngology 96, no. 4 (1987): 449–54. http://dx.doi.org/10.1177/000348948709600420.

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The temporal bones of an individual with documented unilateral Meniere's disease were prepared for light and electron microscopy. a morphometric analysis was performed on hair cells, spiral ganglion cells, dendritic fibers in the osseous spiral lamina, afferent and efferent endings, and afferent synaptic contacts. In the ear with Meniere's disease, we found hair cell damage, including disruption of the cuticular bodies and basalward displacement of some outer hair cells. There was no significant difference in the number of hair cells or spiral ganglion cells on the two sides. There was a signi
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40

Koch, P. J., M. G. Mahoney, G. Cotsarelis, K. Rothenberger, R. M. Lavker, and J. R. Stanley. "Desmoglein 3 anchors telogen hair in the follicle." Journal of Cell Science 111, no. 17 (1998): 2529–37. http://dx.doi.org/10.1242/jcs.111.17.2529.

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Little is known about the function of desmosomes in the normal structure and function of hair. Therefore, it was surprising that mice without desmoglein 3 (the autoantigen in pemphigus vulgaris) not only developed mucous membrane and skin lesions like pemphigus patients, but also developed hair loss. Analysis of this phenotype indicated that hair was normal through the first growth phase (‘follicular neogenesis’). Around day 20, however, when the hair follicles entered the resting phase of the hair growth cycle (telogen), mice with a targeted disruption of the desmoglein 3 gene (DSG3-/-) lost
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41

Rabbitt, R. D., H. E. Ayliffe, D. Christensen, et al. "Evidence of Piezoelectric Resonance in Isolated Outer Hair Cells." Biophysical Journal 88, no. 3 (2005): 2257–65. http://dx.doi.org/10.1529/biophysj.104.050872.

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42

Qi, Mei-Hao, Yang Qiu, Ke-Yong Tian, et al. "Outer hair cells isolation from postnatal Sprague–Dawley rats." World Journal of Otorhinolaryngology - Head and Neck Surgery 5, no. 1 (2019): 14–18. http://dx.doi.org/10.1016/j.wjorl.2018.01.001.

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43

Santos-Sacchi, Joseph, Min Wu, and Seiji Kakehata. "Furosemide alters nonlinear capacitance in isolated outer hair cells." Hearing Research 159, no. 1-2 (2001): 69–73. http://dx.doi.org/10.1016/s0378-5955(01)00321-5.

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44

He, David Z. Z., Jing Zheng, and Peter Dallos. "Development of acetylcholine receptors in cultured outer hair cells." Hearing Research 162, no. 1-2 (2001): 113–25. http://dx.doi.org/10.1016/s0378-5955(01)00376-8.

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45

Tabuchi, Keiji, Shigeki Tsuji, Kazuya Fujihira, Keiko Oikawa, Akira Hara, and Jun Kusakari. "Outer hair cells functionally and structurally deteriorate during reperfusion." Hearing Research 173, no. 1-2 (2002): 153–63. http://dx.doi.org/10.1016/s0378-5955(02)00349-0.

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46

Nenov, Anastas P., Charles Norris, and Richard P. Bobbin. "Outwardly rectifying currents in guinea pig outer hair cells." Hearing Research 105, no. 1-2 (1997): 146–58. http://dx.doi.org/10.1016/s0378-5955(96)00207-9.

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47

Puschner, Birgit, and Jochen Schacht. "Energy metabolism in cochlear outer hair cells in vitro." Hearing Research 114, no. 1-2 (1997): 102–6. http://dx.doi.org/10.1016/s0378-5955(97)00163-9.

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48

Alkon, D. L., R. Etcheberrigaray, and E. Rojas. "Distribution of voltage sensors in mammalian outer hair cells." Biophysical Journal 65, no. 5 (1993): 1755–56. http://dx.doi.org/10.1016/s0006-3495(93)81232-3.

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49

Brownell, W., C. Bader, D. Bertrand, and Y. de Ribaupierre. "Evoked mechanical responses of isolated cochlear outer hair cells." Science 227, no. 4683 (1985): 194–96. http://dx.doi.org/10.1126/science.3966153.

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50

Ohmishi, Sumio, Mitsuyoshi Hara, and Chiyoko Inagaki. "Furosemide-sensitive Cl- channel in cochlear outer hair cells." Japanese Journal of Pharmacology 58 (1992): 267. http://dx.doi.org/10.1016/s0021-5198(19)49294-9.

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