Relevant bibliographies by topics / Ovine placental lactogen (oPL)

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Academic literature on the topic 'Ovine placental lactogen (oPL)'

Author: Grafiati
Published: 4 June 2021
Last updated: 20 February 2023

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Journal articles on the topic "Ovine placental lactogen (oPL)"

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Warren, W. C., R. Liang, G. G. Krivi, N. R. Siegel, and R. V. Anthony. "Purification and structural characterization of ovine placental lactogen." Journal of Endocrinology 126, no. 1 (July 1990): 141–49. http://dx.doi.org/10.1677/joe.0.1260141.

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ABSTRACT Discrepancies exist in the reported purity and biological activity of ovine placental lactogen (oPL), and little structural characterization has been reported. Ovine PL was purified from fetal cotyledonary tissue (day 100 of gestation) by monitoring activity with a recombinant bovine GH (bGH) liver radioreceptor assay. Two hundred grams of tissue yielded 4·2 mg of oPL, with an ∼ 1000-fold purification of oPL specific activity following initial tissue extraction. The oPL was radioiodinated and used in an ovine fetal liver (day 100 of gestation) radioreceptor assay to examine competitiv
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Wooding, F. B., G. Morgan, I. A. Forsyth, G. Butcher, A. Hutchings, S. A. Billingsley, and P. D. Gluckman. "Light and electron microscopic studies of cellular localization of oPL with monoclonal and polyclonal antibodies." Journal of Histochemistry & Cytochemistry 40, no. 7 (July 1992): 1001–9. http://dx.doi.org/10.1177/40.7.1607634.

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Accurate knowledge of placental lactogen localization is fundamental to any hypothesis of its synthesis and secretion. We used locally generated monoclonal and polyclonal antibodies from three separate sources to localize ovine placental lactogen immunoreactivity on light and electron microscope Lowicryl K4M sections of ovine placentomes of 97-145 days of gestation, using immunogold techniques. All antibodies demonstrated that immunoreactivity was exclusively localized in the trophoectoderm binucleate cell Golgi body and granules and in granules in the syncytium derived from binucleate cell mi
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Huyler, S. E., W. R. Butler, A. Grandis, C. Mann, J. P. Figueroa, P. W. Nathanielsz, M. Mitchell, and S. Handwerger. "Stimulation of ovine placental lactogen secretion by arachidonic acid." Journal of Endocrinology 106, no. 1 (July 1985): 43–47. http://dx.doi.org/10.1677/joe.0.1060043.

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ABSTRACT To determine whether arachidonic acid stimulates the secretion of ovine placental lactogen (oPL), arachidonic acid was infused as an intravenous bolus into pregnant ewes and fetuses. Plasma oPL concentrations were determined in mothers and fetuses before and for 5 h after infusion. The administration of 12·5 mg arachidonic acid (0·15−0·2 mg/kg, n = 11 experiments) to the pregnant ewes caused an increase in maternal plasma oPL concentrations of 73·9±15·6% (s.e.m.) and 60·8±18·1% above the pretreatment concentrations at 4 and 5 h respectively P<0·01 in each instance). The infusion of
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Braun, Thorsten, Shaofu Li, Timothy J. M. Moss, John P. Newnham, John R. G. Challis, Peter D. Gluckman, and Deborah M. Sloboda. "Maternal betamethasone administration reduces binucleate cell number and placental lactogen in sheep." Journal of Endocrinology 194, no. 2 (August 2007): 337–47. http://dx.doi.org/10.1677/joe-07-0123.

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The placenta may mediate glucocorticoid-induced fetal growth restriction. Previous studies have examined effects of fetal cortisol in sheep, which reduces placental binucleate cell (BNC) number; the source of ovine placental lactogen (oPL). The effects of maternal GC are unknown. Therefore, this study examined the effects of maternal betamethasone (BET) administration on BNC number, distribution, placental oPL protein levels, and maternal and fetal plasma oPL levels. Pregnant ewes were randomized to receive injections of saline or one (104 days of gestation; dG), two (104 and 111 dG), or three
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Freemark, M., M. Comer, and S. Handwerger. "Placental lactogen and GH receptors in sheep liver: striking differences in ontogeny and function." American Journal of Physiology-Endocrinology and Metabolism 251, no. 3 (September 1, 1986): E328—E333. http://dx.doi.org/10.1152/ajpendo.1986.251.3.e328.

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To determine whether changes in the relative biological potencies of ovine placental lactogen (oPL) and ovine growth hormone (oGH) during development derive from ontogenetic changes in the binding of these hormones to hepatic receptors, we have compared the binding of 125I-oPL and 125I-oGH to hepatic membranes from fetal lambs and pregnant sheep at mid- and late gestation and from postnatal sheep at 1 day to 7 mo of age. Specific high-affinity 125I-oPL binding sites in ovine fetal liver were detected as early as day 70 of gestation (term = 145 days), and the number of fetal 125I-oPL binding si
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Sakal, E., C. Bignon, J. Grosclaude, A. Kantor, R. Shapira, H. Leibovitch, D. Helman, et al. "Large-scale preparation and characterization of recombinant ovine placental lactogen." Journal of Endocrinology 152, no. 2 (February 1997): 317–27. http://dx.doi.org/10.1677/joe.0.1520317.

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Abstract To clone ovine placental lactogen (oPL) cDNA, total RNA from sheep placental cotyledon was reverse transcribed and the single-stranded cDNA was PCR-amplified with 5′ and 3′ primers containing, respectively, Ncol and PstI sites. The oPL cDNA fragment amplified between these two primers extended from A(−1) to the natural stop codon. The PCR product was gel-purified and subcloned into a Puc vector and the insert was sequenced on both strands, revealing several differences relative to the published sequence: S19N, S69N, D129E and R165Q. We assume that these differences can be accounted fo
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Falconer, J., J. A. Owens, E. Allotta, and J. S. Robinson. "Effect of restriction of placental growth on the concentrations of insulin, glucose and placental lactogen in the plasma of sheep." Journal of Endocrinology 106, no. 1 (July 1985): 7–11. http://dx.doi.org/10.1677/joe.0.1060007.

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ABSTRACT The effect of restricting placental growth on maternal glucose, insulin and placental lactogen was investigated in 16 ewes carrying singleton lambs. Uterine caruncles were removed from seven ewes (caruncle ewes) before pregnancy, resulting in reduced placental size and retarded intra-uterine fetal growth. The concentration of insulin in maternal plasma was similar in both control and caruncle ewes. The concentration of glucose was significantly higher in the caruncle than in the control ewes (3·26 ± 0·15 (s.e.m.) mmol/l, number of observations (n) = 9, vs 2·75 ± 0·1, n = 9, P<0·02,
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Ogawa, E., B. H. Breier, M. K. Bauer, and P. D. Gluckman. "Ovine placental lactogen lacks direct somatogenic and anticatabolic actions in the postnatal lamb." Journal of Endocrinology 145, no. 1 (April 1995): 87–95. http://dx.doi.org/10.1677/joe.0.1450087.

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Abstract The metabolic effects of ovine placental lactogen (oPL) alone and in combination with bovine GH (bGH) were investigated in comparison with the identical dose of bGH alone in the well-fed postnatal lamb. The animals were treated by twice daily intramuscular injection for 5 days with oPL (n=7), bGH (n=7) or bGH+oPL (n=7) at a dose of 0·3 mg/kg per day or saline (n=9). bGH and bGH+oPL treatments, but not oPL treatment, resulted in significantly (P<0·01) higher plasma IGF-I levels than saline treatment. The rate of net protein catabolism (NPC) was significantly (P<0·05) reduced to t
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Lea, Richard G., Peter Wooding, Ian Stewart, Lisa T. Hannah, Stephen Morton, Karen Wallace, Raymond P. Aitken, et al. "The expression of ovine placental lactogen, StAR and progesterone-associated steroidogenic enzymes in placentae of overnourished growing adolescent ewes." Reproduction 133, no. 4 (April 2007): 785–96. http://dx.doi.org/10.1530/rep-06-0294.

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Overnourishing pregnant adolescent sheep promotes maternal growth but reduces placental mass, lamb birth weight and circulating progesterone. This study aimed to determine whether altered progesterone reflected transcript abundance forStAR(cholesterol transporter) and the steroidogenic enzymes (Cyp11A1,Hsd3bandCyp17). Circulating and placental expression of ovine placental lactogen (oPL) was also investigated. Adolescent ewes with singleton pregnancies were fed high (H) or moderate (M) nutrient intake diets to restrict or support placental growth. Experiment 1: peripheral progesterone and oPL
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Chan, John S. D., Z. R. Nie, N. G. Seidah, and M. Chrétien. "Purification of ovine placental lactogen (oPL) using high-performance liquid chromatography." FEBS Letters 199, no. 2 (April 21, 1986): 259–64. http://dx.doi.org/10.1016/0014-5793(86)80491-4.

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Dissertations / Theses on the topic "Ovine placental lactogen (oPL)"

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Regnault, Timothy Robert Hume, of Western Sydney Hawkesbury University, Faculty of Agriculture and Horticulture, and School of Agriculture and Rural Development. "Orchestrated partitioning of maternal nutrients during ovine pregnancy." THESIS_FAH_ARD_Regnault_T.xml, 1997. http://handle.uws.edu.au:8081/1959.7/15.

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Ovine placental lactogen (oPL) is postulated to be involved in the repartitioning of maternal nutrients during pregnancy, through its effect on insulin metabolism. Ovine pancreatic insulin responses to exogenous glucose are depressed during pregnancy and this depression becomes more pronounced as gestation advances. In addition, under the hormonal environment of rising oPL and growth hormone (oGH) concentrations, maternal whole body glucose irreversible loss (GIL) increases. The percentage of GIL accounted for by uterine glucose uptake also increases with advancing gestation and increasing lit
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Regnault, Timothy Robert Hume. "Orchestrated partitioning of maternal nutrients during ovine pregnancy." Thesis, View thesis View thesis, 1997. http://handle.uws.edu.au:8081/1959.7/15.

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Ovine placental lactogen (oPL) is postulated to be involved in the repartitioning of maternal nutrients during pregnancy, through its effect on insulin metabolism. Ovine pancreatic insulin responses to exogenous glucose are depressed during pregnancy and this depression becomes more pronounced as gestation advances. In addition, under the hormonal environment of rising oPL and growth hormone (oGH) concentrations, maternal whole body glucose irreversible loss (GIL) increases. The percentage of GIL accounted for by uterine glucose uptake also increases with advancing gestation and increasing lit
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Liang, Rongti. "Molecular cloning and characterization of the ovine placental lactogen gene /." free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9717169.

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Singh, Kuljeet. "Purification, biochemical and somatogenic characterisation of ovine placental lactogen." 1992. http://hdl.handle.net/2292/3247.

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Ovine placental lactogen (oPL) is secreted by the placenta into both the fetal and maternal compartments. Its biological function(s) during pregnancy and the mechanisms involved are still unclear. A purification procedure was developed for oPL from sheep placental cotyledons of late gestation. Four procedures were attempted to obtain homogeneous oPL. Recoveries of oPL and total protein were measured throughout the several procedures using a specific oPL RIA and the Bradford protein estimation respectively. The third and fourth procedures resulted in homogeneous oPL and a partial amino acid seq
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Reports on the topic "Ovine placental lactogen (oPL)"

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Bazer, Fuller W., Arieh Gertler, and Elisha Gootwine. Role of Placental Lactogen in Sheep. United States Department of Agriculture, January 2001. http://dx.doi.org/10.32747/2001.7574339.bard.

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Central problems in sheep and dairy cattle production are reproductive failure due to embryonic/fetal mortality and low birth weights, especially in prolific breeds, and reduced milk yields which adversely affect neonatal survival and economy of production. The sheep placenta expresses lactogenic (ovine placental lactogen, oPL) and somatogenic (ovine placental growth hormone, oGH) hormones. Our research has focused on the biological roles of oPL and oGH in function of the uterine endometrium during gestation and the mammary gland during pregnancy and lactation. Major conclusions were that: ( 1
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Spencer, Thomas E., Elisha Gootwine, Arieh Gertler, and Fuller W. Bazer. Placental lactogen enhances production efficiency in sheep. United States Department of Agriculture, December 2005. http://dx.doi.org/10.32747/2005.7586543.bard.

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The key objectives of this BARD project were to: (1) study long-term effects of immunization of prepubertal ewes against recombinant ovine placental lactogen (roPL) on subsequent birth weights of their lambs and their milk production; (2) optimize the anti-roPL immunization protocol using adjuvant preparations acceptable to producers and regulatory agencies; and (3) determine the physiological mechanism(s) whereby immunization against oPL increases fetal growth and development and mammogenesis. These objectives were based on key findings from a previous BARD project that: (a) immunization of e
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