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Journal articles on the topic 'Ovine pregnancy'

Author: Grafiati

Published: 4 June 2021

Last updated: 20 February 2023

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1

Flanagan, P. G., D. Westmoreland, N. Stallard, I. M. Stokes, and J. Evans. "Ovine chlamydiosis in pregnancy." BJOG: An International Journal of Obstetrics and Gynaecology 103, no. 4 (April 1996): 382–85. http://dx.doi.org/10.1111/j.1471-0528.1996.tb09749.x.

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Honey, Laura. "Dealing with ovine pregnancy toxaemia." In Practice 43, no. 2 (March 2021): 59. http://dx.doi.org/10.1002/inpr.14.

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3

INNES, ELISABETH A., PAUL M. BARTLEY, DAVID BUXTON, and FRANK KATZER. "Ovine toxoplasmosis." Parasitology 136, no. 14 (December 2009): 1887–94. http://dx.doi.org/10.1017/s0031182009991636.

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SUMMARYCongenital infection with Toxoplasma gondii is an important cause of abortion in sheep worldwide. The cat is the definitive host of the parasite, and infected cats may shed millions of oocysts in their faeces resulting in extensive environmental contamination and an important source of infection for grazing herbivorous animals. Studies looking at development of specific antibodies in sheep, as an indicator of exposure to T. gondii, have shown that there is an increase in seroprevalence associated with age indicating that most infections in sheep occur following birth. The stage of gesta

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4

MacLaren, LA, GB Anderson, RH BonDurant, and AJ Edmondson. "Reproductive cycles and pregnancy in interspecific sheep<==>goat chimaeras." Reproduction, Fertility and Development 5, no. 3 (1993): 261. http://dx.doi.org/10.1071/rd9930261.

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The objectives of the current study were to determine whether interspecific sheep<==>goat chimaeras exhibited reproductive cycles of their component species and were capable of maintaining ovine and caprine pregnancies to term. All chimaeras had oestrous cycles and several exhibited characteristics of both ewes and does, including short, 6-7-day cycles. Sixteen caprine pregnancies were confirmed in eight sheep<==>goat and one hybrid<==>sheep chimaera from 21 embryo transfers; of these, six appeared normal by ultrasonographic examination during Weeks 5 or 6, but none progresse

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Hull, A. D., D. M. Long, L. D. Longo, and W. J. Pearce. "Pregnancy-induced changes in ovine cerebral arteries." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 262, no. 1 (January 1, 1992): R137—R143. http://dx.doi.org/10.1152/ajpregu.1992.262.1.r137.

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We examined the effects of pregnancy on the ovine cerebral vasculature by comparing several characteristics of isolated endothelium-intact segments of three intracranial arteries including the middle cerebral (MCA), posterior communicating (PC), and basilar (BAS) arteries taken from pregnant sheep (138-143 days gestation, term approximately 145 days) and nonpregnant controls. For comparison, segments of the extracranial common carotid (COM) artery were also studied. With pregnancy, vessel water content increased (5.4-5.8%) in all arteries except the PC. Additionally, cellular protein content i

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Keller-Wood, Maureen. "ACTH responses to CRF and AVP in pregnant and nonpregnant ewes." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 274, no. 6 (June 1, 1998): R1762—R1768. http://dx.doi.org/10.1152/ajpregu.1998.274.6.r1762.

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During both ovine and human pregnancy plasma cortisol is increased. In human pregnancy the placenta secretes corticotropin-releasing factor (CRF), but pituitary responses to CRF are decreased. However, in ovine pregnancy there is no measurable placental secretion of CRF. This study tests for changes in pituitary responsiveness to CRF or AVP. Pregnant and nonpregnant ewes were infused with saline or CRF at three doses (3, 9, 45 μg/h), with or without coinfusion of AVP (9 μg/h). AVP infusion increased plasma AVP to ∼250 pg/ml. CRF infusions increased plasma CRF from ∼25 to 50, 150, and 850 pg/ml

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Simmons, Rebecca M., David W. Erikson, Jinyoung Kim, Robert C. Burghardt, Fuller W. Bazer, Greg A. Johnson, and Thomas E. Spencer. "Insulin-Like Growth Factor Binding Protein-1 in the Ruminant Uterus: Potential Endometrial Marker and Regulator of Conceptus Elongation." Endocrinology 150, no. 9 (June 4, 2009): 4295–305. http://dx.doi.org/10.1210/en.2009-0060.

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Abstract Establishment of pregnancy in ruminants requires conceptus elongation and production of interferon-τ (IFNT), the pregnancy recognition signal that maintains ovarian progesterone (P4) production. These studies determined temporal and spatial alterations in IGF binding protein (IGFBP)-1 and IGFBP3 in the ovine and bovine uterus; effects of P4 and IFNT on their expression in the ovine uterus; and effects of IGFBP1 on ovine trophectoderm cell proliferation, migration, and attachment. IGFBP1 and IGFBP3 were studied because they are the only IGFBPs specifically expressed by the endometrial

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8

Santos, A. C., G. R. Arthur, H. Pedersen, H. O. Morishima, M. Finster, and B. G. Covino. "Systemic Toxicity of Ropivacaine during Ovine Pregnancy." Anesthesiology 75, no. 1 (July 1, 1991): 137–41. http://dx.doi.org/10.1097/00000542-199107000-00022.

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9

Zoli, A. P., J. F. Beckers, and F. Ectors. "Isolation of an ovine pregnancy specific protein." Theriogenology 33, no. 1 (January 1990): 366. http://dx.doi.org/10.1016/0093-691x(90)90790-z.

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10

Magness, R. R., M. D. Mitchell, and C. R. Rosenfeld. "Uteroplacental production of eicosanoids in ovine pregnancy." Prostaglandins 39, no. 1 (January 1990): 75–88. http://dx.doi.org/10.1016/0090-6980(90)90096-e.

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11

Guo, Yanhua, Jiachen Bai, Zhenliang Zhang, Yucheng Liu, Shouliang Lu, Changbin Liu, Jianhong Ni, et al. "Pregnancy of Cryopreserved Ovine Embryos at Different Developmental Stages." Cryoletters 43, no. 5 (September 1, 2022): 269–75. http://dx.doi.org/10.54680/fr22510110512.

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BACKGROUND: Developmental stage and cryopreservation method have significant impact on the pregnancy rate after transfer of embryos produced in vivo. OBJECTIVE: To determine the pregnancy outcomes from ovine embryos cryopreserved at different developmental stages. MATERIALS AND METHODS: Embryos at different developmental stages were obtained from donor ewes through simultaneous estrus treatment and laparoscopic artificial insemination. Embryos, either cryopreserved via vitrification or slow freezing method, were implanted into recipient ewes. The pregnancy rate was determined 35 days after tra

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12

Tekin, Şaban, and Peter J. Hansen. "Natural Killer-Like Cells in the Sheep: Functional Characterization and Regulation by Pregnancy-Associated Proteins." Experimental Biology and Medicine 227, no. 9 (October 2002): 803–11. http://dx.doi.org/10.1177/153537020222700913.

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Natural killer (NK) cells represent an important component of the innate immune system. In ruminants there are few reports regarding presence or characterization of NK cells. Although absence of expression of major histocompatibility complex proteins on ovine trophoblast makes it potentially a target for NK cells, little is known about regulation of NK cells by products of pregnancy in sheep. Objectives of the present study were to determine whether cells with characteristics of NK cells exist in preparations of ovine peripheral blood lymphocytes (PBL) and endometrial epithelial cells (EEC) an

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Spencer, TE, GH Graf, and FW Bazer. "Sulfated glycoprotein-1 (SGP-1) expression in ovine endometrium during the oestrous cycle and early pregnancy." Reproduction, Fertility and Development 7, no. 5 (1995): 1053. http://dx.doi.org/10.1071/rd9951053.

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This study determined effects of day of oestrous cycle and early pregnancy on sulfated glycoprotein-1 (SGP-1) expression in ovine endometrium. A 364-bp clone of the ovine SGP-1 mRNA was amplified from reverse transcribed Day-15 cyclic endometrial mRNA using the polymerase chain reaction (PCR) and primers specific for the rat SGP-1 mRNA sequence. Nucleotide sequence of the ovine SGP-1 cDNA shared significant identity with rat SGP-1 and human prosaposin. Ewes (n = 40) were hysterectomized on either Day 1, 6, 11, 13 or 15 of the oestrous cycle or on Day 11, 13, 15, 17 or 25 of early pregnancy. To

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Kelly, J., D. Kleemann, M. Kuwayama, and S. Walker. "88 PREGNANCY RATES FOR IN VITRO AND IN VIVO PRODUCED OVINE EMBRYOS VITRIFIED USING THE MINIMUM VOLUME COOLING CRYOTOP METHOD." Reproduction, Fertility and Development 17, no. 2 (2005): 194. http://dx.doi.org/10.1071/rdv17n2ab88.

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Previously we reported that, using the minimum volume cooling (MVC) cryotop vitrification method, in vitro-produced ovine and bovine embryo survival after thawing was similiar to that of fresh embryos (Kelly et al. 2004 Reprod. Fert. Dev. 16, 172). While survival of vitrified embryos after thawing can be indicative of embryo viability, this assessment does not always correlate with embryo survival after transfer. This study assesses the effect of vitrification using the MVC cryotop method on the survival after transfer of in vitro- and in vivo-produced ovine embryos. Fresh or vitrified Day 6 o

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15

Annibale, D. J., C. R. Rosenfeld, J. T. Stull, and K. E. Kamm. "Protein content and myosin light chain phosphorylation in uterine arteries during pregnancy." American Journal of Physiology-Cell Physiology 259, no. 3 (September 1, 1990): C484—C489. http://dx.doi.org/10.1152/ajpcell.1990.259.3.c484.

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During pregnancy, the ovine uterine artery changes from a low- to a high-stress artery. We investigated the hypotheses that the increased stress reflects alterations in vessel wall cellularity, smooth muscle cell contractile protein contents, or activation properties. Uterine artery diameter increased during pregnancy, whereas the fractional cellular composition and thickness of the muscularis were unchanged. Results of morphometry suggest that vessel growth is associated with cell elongation. Uterine arteries from pregnant ewes had greater protein contents than those from nonpregnant ewes (10

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16

Parkinson, T. J., H. J. Stewart, M. G. Hunter, D. S. C. Jones, D. C. Wathes, and A. P. F. Flint. "Evidence against a role for blastocyst-secreted oxytocin in early pregnancy maintenance in sheep." Journal of Endocrinology 130, no. 3 (September 1991): 443—NP. http://dx.doi.org/10.1677/joe.0.1300443.

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ABSTRACT Analysis of ovine conceptus RNA by slot blotting, Northern analysis and nested polymerase chain reaction failed to detect oxytocin–neurophysin prohormone mRNA. Probes used hybridized with both the 3' end of the prohormone mRNA and the oxytocin-coding sequence. Northern analysis of bovine and porcine conceptus RNA was also negative, and polymerase chain reaction demonstrated oxytocin–neurophysin mRNA in ovine corpus luteum, but not in human corpus luteum or decidua, or in ovine endometrium. Infusion of oxytocin into the uterine lumen in cyclic ewes between days 9 and 19 or 20 after oes

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17

Cha, Sang C., Graham W. Aberdeen, Suhayla Mukaddam-Daher, Edmond W. Quillen, and Bahij S. Nuwayhid. "Autoregulation of Renal Blood Flow During Ovine Pregnancy." Hypertension in Pregnancy 12, no. 1 (January 1993): 71–83. http://dx.doi.org/10.3109/10641959309031054.

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Lacroix, M. C., G. Charpigny, and P. Reinaud. "IS OXYTOCIN OF CONCEPTUS ORIGIN INVOLVED IN INHIBITION OF LUTEAL REGRESSION IN EARLY PREGNANCY IN EWES?" Journal of Endocrinology 118, no. 3 (September 1988): R17—R20. http://dx.doi.org/10.1677/joe.0.118r017.

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ABSTRACT This study describes the presence in and production by the ovine conceptus of an oxytocin-like peptide during the early stages of development. Oxycotin was measured by radioimmunoassay in ovine conceptuses from days 14 to 30 of pregnancy. Tissue concentrations of oxytocin increased from day 14 (24.8 ± 5 pg/100 mg) until day 19 (122.9 ± 52 pg/100 mg) and then decreased (3 ± 1 pg /100 mg). Oxytocin was released into culture medium by day-15 ovine conceptuses at a rate of 262 ± 55 pg/24 h. Reverse-phase high-performance liquid chromatography (HPLC) analysis of peptides extracted from a p

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19

Charles, David. "How to: ovine clinical examination." Livestock 28, no. 1 (January 2, 2023): 41–49. http://dx.doi.org/10.12968/live.2023.28.1.41.

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When asked to consider the core activities of a large animal practitioner, many would conjure up images of vets standing at the back end of a cow performing rectal palpation, or ultrasound for pregnancy diagnosis. However, the essential skill in any large animal practitioner's toolbox is the ability to perform a thorough and accurate clinical examination. This article discusses approaches to the ovine clinical examination, the nuances and differences expected for rams and neonates.

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Arikan, Ş., and A. A. Yigit. "Size distribution of steroidogenic and non-steroidogenic ovine luteal cells throughout pregnancy." Animal Science 75, no. 3 (December 2002): 427–32. http://dx.doi.org/10.1017/s1357729800053194.

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AbstractThe present study examines the size distribution of ovine steroidogenic and non-steroidogenic luteal cells throughout pregnancy. Cells were isolated from corpora lutea collected from early (< 8 weeks), mid (9 to 14 weeks) or late (15 to 18 weeks) stages of pregnancy. Cells were stained for 3β-hydroxysteroid dehydrogenase (3β-HSD) activity, a marker for steroidogenic cells. Both 3β-HSD positive and β-HSD negative cells covered a wide spectrum of size ranging from 7 to 37 μm in diameter. There was a significant increase (P > 0·01) in mean diameter of non-steroidogenic luteal cells

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21

Kiyma, Z., M. Hitit, C. Ozel, G. Sen, M. Kose, A. Guzeloglu, M. Kaya, et al. "121 EXPRESSION OF HYALURONAN SYSTEM COMPONENTS IN THE EARLY PREGNANT OVINE ENDOMETRIUM." Reproduction, Fertility and Development 27, no. 1 (2015): 152. http://dx.doi.org/10.1071/rdv27n1ab121.

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Hyaluronan (HA; hyaluronic acid), a member of glycosaminoglycans (GAG) family, is the main polysaccharide and is expressed in almost all tissues including those of the reproductive tract. Three different hyaluronic acid-synthase (HAS) enzymes, HAS1, HAS2, and HAS3, synthesise HA. The action of HA depends on its molecular size and its cell surface receptor (CD44). Hyaluronidases (HYAL) are a group of enzymes, HYAL-1 and HYAL-2, that degrade HA. Roles of the HA system in reproductive events include oocyte maturation, fertilization, and implantation. It is also known that ovarian steroids, specif

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22

Keller-Wood, M. "Corticotropin responses to hypoglycemia and hypotension during ovine pregnancy." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 266, no. 1 (January 1, 1994): R180—R187. http://dx.doi.org/10.1152/ajpregu.1994.266.1.r180.

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The adrenocorticotropic hormone (ACTH) responses to hypoglycemia and to hypotension were compared in pregnant and nonpregnant ewes. In the first study pregnant and nonpregnant ewes were each subjected to hypoglycemia induced by injection of 0.05, 0.10, or 0.25 U regular insulin/kg body wt or to saline infused as a control. In the second study pregnant and nonpregnant ewes were subjected to hypotension induced by the infusion of 2.5, 5.0, or 10.0 micrograms nitroprusside.kg-1.min-1 or to dextrose infused as a control. ACTH responses to hypoglycemia were significantly increased in the pregnant e

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Milisits-Németh, Tímea, Orsolya Gabriella Balogh, István Egerszegi, László Kern, R. Garth Sasser, and György Gábor. "Detection of pregnancy in sheep using an ELISA for pregnancy-specific protein B." Acta Veterinaria Hungarica 66, no. 2 (June 2018): 329–36. http://dx.doi.org/10.1556/004.2018.029.

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The early detection of pregnancy and the determination of fetal numbers have economic benefits in sheep production because of the seasonal breeding patterns where missing a breeding opportunity means the loss of one productive year. The purpose of this study was to evaluate the efficacy of the B6-HRP ELISA for ovine pregnancy-specific protein B (oPSPB) measurement in the detection of pregnancy and estimation of fetal numbers in different sheep breeds. BioPRYN® ELISA assay kit was used for the detection of pregnancy in the experimental animals. Ninety-three ewes of three breeds (British Milkshe

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Keller-Wood, M. "Vasopressin response to hyperosmolality and hypotension during ovine pregnancy." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 266, no. 1 (January 1, 1994): R188—R193. http://dx.doi.org/10.1152/ajpregu.1994.266.1.r188.

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The arginine vasopressin (AVP) responses to hyperosmolality and to hypotension were compared in pregnant and nonpregnant ewes. When the responses to infusion of normal or hypertonic saline were compared, plasma AVP and Na+ concentrations were lower in pregnant ewes than nonpregnant ewes, but the relation between plasma AVP and Na+ concentrations was not altered in the pregnant state. In a second study the AVP response to hypotension, induced by the infusion of 2.5, 5.0, or 10.0 micrograms nitroprusside.kg-1.min-1, was compared in pregnant and nonpregnant ewes. Despite significantly lower mean

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Yang, Ling, Xu Han, Leying Zhang, Ning Li, Zimo Zhao, and Jiachen Bai. "Changes in expression of prostaglandin synthase in ovine liver during early pregnancy." Canadian Journal of Animal Science 100, no. 3 (September 1, 2020): 432–39. http://dx.doi.org/10.1139/cjas-2019-0171.

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Liver can function as part of the innate and adaptive immune systems. We hypothesize that prostaglandins participate in the regulation of hepatic immune function during early pregnancy in sheep. The objective of this study was to elucidate expression of prostaglandin synthase in ovine liver during early pregnancy. Ovine livers were sampled on day 16 of the estrous cycle, and days 13, 16, and 25 of pregnancy, and the expression of prostaglandin synthases, including prostaglandin-endoperoxide synthase 1 (PTGS1), PTGS2, prostaglandin E synthase (PTGES), and aldo-keto reductase family 1, member B1

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Soares, MC, JL Servely, C. Puissant, P. Bolifraud, MC Lacroix, B. Schaeffer, and G. Kann. "Ovine chorionic somatomammotrophin (oCS) production by isolated cotyledon cells from sheep in early and mid gestation: auto-regulation by recombinant oCS." Journal of Endocrinology 161, no. 2 (May 1, 1999): 289–98. http://dx.doi.org/10.1677/joe.0.1610289.

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We report the ability of sheep placental cotyledonary cells, isolated at different periods of pregnancy (40 to 90 days) to produce ovine chorionic somatomammotrophin (oCS) in in vitro culture conditions. This oCS production increased gradually with stage of pregnancy. Endogenous oCS net production by isolated placental cells was increased, in a dose-dependent manner, by addition of recombinant oCS (roCS). This effect was not observed after addition of recombinant ovine growth hormone. The roCS effect was more potent on cells collected during early pregnancy. Specific immunoprecipitation of oCS

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Annibale, D. J., C. R. Rosenfeld, and K. E. Kamm. "Alterations in vascular smooth muscle contractility during ovine pregnancy." American Journal of Physiology-Heart and Circulatory Physiology 256, no. 5 (May 1, 1989): H1282—H1288. http://dx.doi.org/10.1152/ajpheart.1989.256.5.h1282.

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During pregnancy, sheep develop attenuated systemic and uterine vascular responsiveness to alpha-adrenergic stimulation. To determine whether this reflects altered vascular smooth muscle function, we studied the responsiveness of smooth muscle isolated from systemic and uterine arteries to KCl and phenylephrine. Uterine, renal, and carotid arteries were collected from nonpregnant, pregnant (131 +/- 2 days, +/- SD), and late postpartum (144 +/- 4 days) ewes; endothelium was removed and open rings were hung for measurement of isometric force. There were no differences in concentration-response r

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El Amiri, B., A. Karen, J. Sulon, N. Melo de Sousa, AV Alvarez-Oxiley, Y. Cognié, O. Szenci, and JF Beckers. "Measurement of Ovine Pregnancy-Associated Glycoprotein (PAG) During Early Pregnancy in Lacaune Sheep." Reproduction in Domestic Animals 42, no. 3 (June 2007): 257–62. http://dx.doi.org/10.1111/j.1439-0531.2006.00761.x.

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Sandra, Olivier, Isabelle Bataillon, Pascale Roux, Jacques Martal, Gilles Charpigny, Pierrette Reinaud, Philippe Bolifraud, Guy Germain, and Kaïs H. Al-Gubory. "Suppressor of cytokine signalling (SOCS) genes are expressed in the endometrium and regulated by conceptus signals during early pregnancy in the ewe." Journal of Molecular Endocrinology 34, no. 3 (June 2005): 637–44. http://dx.doi.org/10.1677/jme.1.01667.

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It is established that the conceptus–endometrium dialogue involves cytokines, growth factors and hormones. Given the crucial functions of the suppressor of cytokine signaling (SOCS) family proteins in cytokine signalling, we analyzed the expression and the regulation of CIS and SOCSs 1–3 transcripts during early pregnancy in the ovine endometrium. An overall stimulation of the SOCS transcripts was described in the pregnant ewes with two specific patterns. Unilaterally pregnant ewes confirmed the conceptus-produced factors as regulators of the SOCSs 1–3 expression at day 16 of pregnancy. Intrau

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Philipps, A. F., T. S. Rosenkrantz, and J. Raye. "Consequences of Perturbations of Fetal Fuels in Ovine Pregnancy." Diabetes 34, Supplement_2 (June 1, 1985): 32–35. http://dx.doi.org/10.2337/diab.34.2.s32.

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Mukaddam-Daher, S., J. Gutkowska, B. S. Nuwayhid, and E. W. Quillen. "Metabolic clearance of atrial natriuretic factor in ovine pregnancy." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 267, no. 5 (November 1, 1994): R1413—R1420. http://dx.doi.org/10.1152/ajpregu.1994.267.5.r1413.

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Plasma atrial natriuretic factor (ANF) is normally released into the circulation primarily by volume expansion and atrial distension, but we have shown that plasma ANF is elevated in pregnant sheep before volume expansion. Because alterations in the metabolic clearance of ANF could lead to elevated plasma ANF levels, the present study was designed to determine the pharmacokinetics of plasma ANF in pregnant sheep. Chronically instrumented nonpregnant and pregnant sheep received intravenous injections of monoiodinated human ANF (125I-hANF). Plasma decay curves of 125I-hANF followed a biexponenti

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Cha, S. C., G. W. Aberdeen, S. Mukaddam-Daher, E. W. Quillen, and B. S. Nuwayhid. "Tubular handling of fluid and electrolytes during ovine pregnancy." American Journal of Physiology-Renal Physiology 265, no. 2 (August 1, 1993): F278—F284. http://dx.doi.org/10.1152/ajprenal.1993.265.2.f278.

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Pregnancy is characterized by progressive water and sodium accumulation and increases in renal blood flow (RBF) and glomerular filtration rate (GFR). However, the influence of the different nephron segments on the increased tubular reabsorption is controversial. Consequently, four nonpregnant and five pregnant sheep were studied, after chronic instrumentation, to assess salt and water reabsorption in the proximal and distal tubules under basal and volume-loaded conditions. Lithium clearance was used as a marker for proximal tubular reabsorption. Volume loading was achieved by the rapid adminis

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Janowiak, Mary A., Ronald R. Magness, Deirdre A. Habermehl, and Ian M. Bird. "Pregnancy Increases Ovine Uterine Artery Endothelial Cyclooxygenase-1 Expression*." Endocrinology 139, no. 2 (February 1, 1998): 765–71. http://dx.doi.org/10.1210/endo.139.2.5739.

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MITCHELL, G. M., and B. F. STRATFORD. "The morphology of the ovine placenta in pregnancy toxaemia." Australian Veterinary Journal 64, no. 7 (July 1987): 221–23. http://dx.doi.org/10.1111/j.1751-0813.1987.tb15189.x.

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Curry, Steven C., G. Randall Bond, Robert Raschke, David Tellez, and Donna Wiggins. "An ovine model of maternal iron poisoning in pregnancy." Annals of Emergency Medicine 19, no. 6 (June 1990): 632–38. http://dx.doi.org/10.1016/s0196-0644(05)82466-7.

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Cox, B. E., M. A. Ipson, P. W. Shaul, K. E. Kamm, and C. R. Rosenfeld. "Myometrial angiotensin II receptor subtypes change during ovine pregnancy." Journal of Clinical Investigation 92, no. 5 (November 1, 1993): 2240–48. http://dx.doi.org/10.1172/jci116827.

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Lacroix, MC, H. Jammes, and G. Kann. "Occurrence of a growth hormone-releasing hormone-like messenger ribonucleic acid and immunoreactive peptide in the sheep placenta." Reproduction, Fertility and Development 8, no. 3 (1996): 449. http://dx.doi.org/10.1071/rd9960449.

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Growth hormone releasing factor (GHRH) has been described in the rat, mouse and human placentae. This study reports the presence of an immunoreactive GHRH activity (IR-GHRH) in the ovine placenta. This activity was detected by radioimmunoassay from day 50 (D50) until the end of pregnancy. Higher IR-GHRH concentration in placental tissue was observed on days 100 (543 +/- 123 pg/g) and 140 (550 +/- 62 pg/g) and, when compared with the GHRH content of the ovine hypothalamus (1.2 ng/hypothalamus), represents a considerable amount of GHRH per placenta (a mean of 200 ng). Perifused placenta explants

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Yang, Ling, Luyu Wang, Jiaxuan Wu, Haichao Wang, Gengxin Yang, and Leying Zhang. "Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy." Animals 11, no. 11 (November 8, 2021): 3183. http://dx.doi.org/10.3390/ani11113183.

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During early gestation in humans, complement regulation is essential for normal fetal growth. It is supposed that a complement pathway participates in maternal splenic immune regulation at the early stage of gestation in ewes. The aim of this study was to analyze the effects of early pregnancy on the expression of complement components in the maternal spleen of ewes. In this study, ovine spleens were sampled on day 16 of nonpregnancy, and days 13, 16 and 25 of gestation. RT-qPCR, Western blot and immunohistochemical analysis were used to detect the changes in expression of complement component

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Zhang, Leying, Chen Zhuang, Zimo Zhao, Ning Li, Jiachen Bai, and Ling Yang. "Effect of early pregnancy on the expression of progesterone receptor and progesterone-induced blocking factor 1 in ovine liver." Czech Journal of Animal Science 64, No. 7 (July 17, 2019): 317–23. http://dx.doi.org/10.17221/21/2019-cjas.

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Liver plays important roles in the innate and adaptive immunity, and contributes to the maternal immune adjustments during pregnancy. Progesterone (P4) has key effects on immunomodulation of the maternal uterus during pregnancy. In this study, livers were obtained at day 16 of the estrous cycle and at days 13, 16 and 25 of pregnancy (n = 6 for each group) in ewes. The effects of early pregnancy on the expression of P4 receptor (PGR) and progesterone-induced blocking factor 1 (PIBF1) were analysed through RT-qPCR assay, Western blot and immunohistochemistry analysis. Our results showed that the

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Rovani, Monique Tomazele, Alfredo Skrebsky Cezar, Melânia Lazzari Rigo, Bernardo Garziera Gasperin, Janduí Escarião da Nóbrega Júnior, Fabrício Dias Torres, Paulo Bayard Dias Gonçalves, and Rogério Ferreira. "Evaluation of a bovine pregnancy-associated glycoprotein enzyme-linked immunosorbent assay kit for serological diagnosis of pregnancy in sheep." Ciência Rural 46, no. 2 (November 27, 2015): 362–67. http://dx.doi.org/10.1590/0103-8478cr20150270.

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ABSTRACT: Pregnancy diagnosis is an important tool for farm management. Ultrasonography is the main technique used for pregnancy diagnosis in ewes. As an alternative, radioimmunoassay (RIA) allows accurate and early detection of pregnancy-associated glycoproteins (PAGs) in sheep blood. However, radioactive-based techniques, as RIA, have been increasingly inadvisable due to environmental risk. Homology between ovine and bovine PAGs is high, and ELISA kits used for PAGs detection in cattle are safer than RIA. Thus, this study aimed to evaluate the feasibility of PAGs detection for pregnancy diag

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Palmarini, Massimo, C. Allison Gray, Karen Carpenter, Hung Fan, Fuller W. Bazer, and Thomas E. Spencer. "Expression of Endogenous Betaretroviruses in the Ovine Uterus: Effects of Neonatal Age, Estrous Cycle, Pregnancy, and Progesterone." Journal of Virology 75, no. 23 (December 1, 2001): 11319–27. http://dx.doi.org/10.1128/jvi.75.23.11319-11327.2001.

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ABSTRACT The ovine genome contains 15 to 20 copies of endogenous retroviruses (enJSRVs) highly related to the oncogenic jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus. enJSRVs are highly expressed in the endometrial lumenal epithelia (LE) and glandular epithelia (GE) of the ovine uterus. The effects of neonatal age, estrous cycle, pregnancy, and progesterone on expression of enJSRVs in the ovine uterus were determined. Expression of enJSRV RNAs was absent from the uterus of ewes at birth, but enJSRV RNAs were expressed specifically in the LE and developing GE from postnatal

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MUSA, S. I., K. O. YAHAYA, and A. YAHAYA. "COMPUTATIONAL ANALYSIS OF FUNCTIONAL EFFECTS OF SINGLE NUCLEOTIDE POLYMORPHISM ON OVINE INTERFERON TAU." FUDMA Journal of Agriculture and Agricultural Technology 8, no. 1 (September 15, 2022): 26–29. http://dx.doi.org/10.33003/jaat.2022.0801.071.

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The objective of this study was to evaluate the functional effects of Single Nucleotide Polymorphism(SNP) in ovine interferon tau using computational methods. Data on ovine interferon was retrieved from data base of National Center for Biotechnology Information ((GenBank). Functional effects of substitution of Leucin by Proline at position 26 of interferon protein chain was predicted using Protein Variation Effect Analyser, Panther server and Poly phen 2 (Polymorphism phenotyping). Provean score of - 5.828 was obtained, indicating a deleterious effect of the amino acid substitution. Panther se

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Song, Gwonhwa, Jo-Ann G. W. Fleming, Jinyoung Kim, Thomas E. Spencer та Fuller W. Bazer. "Pregnancy and interferon τ regulate DDX58 and PLSCR1 in the ovine uterus during the peri-implantation period". REPRODUCTION 141, № 1 (січень 2011): 127–38. http://dx.doi.org/10.1530/rep-10-0348.

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Interferon τ (IFNT), the pregnancy recognition signal in ruminants, abrogates the luteolytic mechanism for maintenance of the corpus luteum for production of progesterone (P4). This study examined the expression of DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) and phospholipid scramblase 1 (PLSCR1) mRNAs in the ovine uterus as these genes were increased most in 2fTGH (STAT1 positive) cells by IFNT. The results of this study indicated that IFNT regulates expression ofDDX58andPLSCR1mRNAs in the ovine uterus, which confirmed the results of thein vitrotranscriptional profiling experiment with

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Salamonsen, LA, and JK Findlay. "Regulation of endometrial prostaglandins during the menstrual cycle and in early pregnancy." Reproduction, Fertility and Development 2, no. 5 (1990): 443. http://dx.doi.org/10.1071/rd9900443.

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Prostaglandins are important regulators of endometrial function. In turn, their secretion is controlled by endocrine and paracrine mediators. Cyclical effects of ovarian oestrogen and progesterone throughout the menstrual or oestrous cycle result in overall higher levels of prostaglandin release during the secretory or luteal phase of the cycle than during the proliferative phase. Potential paracrine regulators of endometrial origin include cytokines, growth factors and histamine, some of which may arise from infiltrating cells. Embryo-derived factors can regulate endometrial prostaglandin rel

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Quillen, E. W., and B. S. Nuwayhid. "Steady-state arterial pressure-urinary output relationships during ovine pregnancy." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 263, no. 5 (November 1, 1992): R1141—R1146. http://dx.doi.org/10.1152/ajpregu.1992.263.5.r1141.

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To determine the effects of long-term changes in sodium intake on mean arterial pressure (MAP) regulation during pregnancy, nonpregnant (n = 16) and 110- to 140-day pregnant (n = 13) ewes received total daily sodium intakes of 10, 30, 100, 400, and 1,200 mmol for 7 days. The sheep were housed in metabolism cages and MAP was monitored 24 h/day. Urinary sodium excretion (UNaV) followed changes in sodium intake, with steady-state levels being achieved with similar degrees of rapidity (2-3 days) in nonpregnant and pregnant sheep. At 10 mmol/day sodium intake, MAP was lower (79 +/- 1 vs. 82 +/- 2 m

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Wang, Yujiao, Xu Han, Leying Zhang, Nan Cao, Lidong Cao, and Ling Yang. "Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen." Animals 9, no. 11 (October 30, 2019): 882. http://dx.doi.org/10.3390/ani9110882.

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Interferon-tau is a maternal recognition factor in ruminant species, and spleen plays an essential role in regulating innate and adaptive immune responses. However, it is not fully understood that early pregnancy induces expression of interferon stimulated genes (ISGs) in the spleen during early pregnancy in ewes. In this study, spleens were collected from ewes at day 16 of the estrous cycle, and on days 13, 16, and 25 of gestation (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to detect the expression of signal transducer and activator of transcr

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Romero, Jared J., Alfredo Q. Antoniazzi, Natalia P. Smirnova, Brett T. Webb, Fang Yu, John S. Davis, and Thomas R. Hansen. "Pregnancy-associated genes contribute to antiluteolytic mechanisms in ovine corpus luteum." Physiological Genomics 45, no. 22 (November 15, 2013): 1095–108. http://dx.doi.org/10.1152/physiolgenomics.00082.2013.

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The hypothesis that ovine luteal gene expression differs due to pregnancy status and day of estrous cycle was tested. RNA was isolated from corpora lutea (CL) on days 12 and 14 of the estrous cycle (NP) or pregnancy (P) and analyzed with the Affymetrix bovine microarray. RNA also was isolated from luteal cells on day 10 of estrous cycle that were cultured for 24 h with luteolytic hormones (OXT and PGF) and secretory products of the conceptus (IFNT and PGE2). Differential gene expression (>1.5-fold, P < 0.05) was confirmed using semiquantitative real-time PCR. Serum progesterone concentra

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Galio, Laurent, Stéphanie Droineau, Patrick Yeboah, Hania Boudiaf, Stephan Bouet, Sandrine Truchet, and Eve Devinoy. "MicroRNA in the ovine mammary gland during early pregnancy: spatial and temporal expression of miR-21, miR-205, and miR-200." Physiological Genomics 45, no. 4 (February 15, 2013): 151–61. http://dx.doi.org/10.1152/physiolgenomics.00091.2012.

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The mammary gland undergoes extensive remodeling between the beginning of pregnancy and lactation; this involves cellular processes including cell proliferation, differentiation, and apoptosis, all of which are under the control of numerous regulators. To unravel the role played by miRNA, we describe here 47 new ovine miRNA cloned from mammary gland in early pregnancy displaying strong similarities with those already identified in the cow, human, or mouse. A microarray study of miRNA variations in the adult ovine mammary gland during pregnancy and lactation showed that 100 miRNA are regulated

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Gray, C. Allison, Kathrin A. Dunlap, Robert C. Burghardt, and Thomas E. Spencer. "Galectin-15 in ovine uteroplacental tissues." Reproduction 130, no. 2 (August 2005): 231–40. http://dx.doi.org/10.1530/rep.1.00637.

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Galectin-15 is the newest member of a secreted β-galactoside-binding lectin family. The galectin-15 gene is expressed specifically by the endometrial luminal epithelium (LE) and superficial ductal glandular epithelium (sGE) of the ovine uterus. The proposed extracellular role of secreted galec7tin-15 is to regulate implantation and placentation by functioning as a heterophilic cell adhesion molecule between the conceptus trophectoderm and endometrial LE, while that of intracellular galectin-15 is to regulate cell survival, differentiation and function. The present study determined galectin-15

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Wong, M. H., and G. E. Rice. "Characterisation of acyltransferase activity in ovine placental tissue during pregnancy and at the time of labour." Journal of Endocrinology 148, no. 2 (February 1996): 241–47. http://dx.doi.org/10.1677/joe.0.1480241.

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Abstract Although it is well established that the formation of eicosanoids by ovine intrauterine tissues increases during pregnancy and at the time of labour, the biochemical mechanisms involved remain to be clearly established. In this study, we tested the hypothesis that the gestational and labour-associated increases in eicosanoid formation are associated with a reduction in the activity of the reacylating enzyme, acyl Coenzyme A lysophosphatide acyltransferase (LAT). To evaluate this proposal, in vitro LAT activity was quantified in ovine placenta (cotyledons) obtained during pregnancy (85

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