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Journal articles on the topic 'P. Aeruginosa Genotypic Characteristics'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Ghorbani, Ghasem, Ebrahim Rahimi, and Amir Shakerian. "Antibiotic resistance’s Genotypic and Phenotypic Characteristics and the Frequency of Virulence Factors in P. aeruginosa Isolates Isolated from Water Samples in Iran." BioMed Research International 2022 (September 30, 2022): 1–7. http://dx.doi.org/10.1155/2022/7076433.

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Pseudomonas aeruginosa is a pathogenic bacterium that can contaminate water. In this study, 430 water samples were evaluated for P. aeruginosa, antibiotic resistance, and the abundance of virulence factors. P. aeruginosa was isolated from 28 (6.51%) water samples. Among the types of water, well and spring water showed the highest P. aeruginosa with, respectively, 20 (15.6%) and 5 (8.06%) positive samples per type of samples. Drinking water and mineral water showed minor contamination with P. aeruginosa. The prevalence of antibiotic resistance against meropenem, imipenem, erythromycin, gentamic

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Koutsogiannou, M., E. Drougka, A. Liakopoulos, et al. "Comparative epidemiological study of Pseudomonas aeruginosa isolates during two time periods in a tertiary care hospital." ACTA MICROBIOLOGICA HELLENICA 59, no. 4 (2014): 43–56. https://doi.org/10.5281/zenodo.10017365.

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Pseudomonas aeruginosa is a nosocomial pathogen of clinical importance carrying virulence and antibiotic resistance determinants. In the present study, phenotypic and genotypic characteristics of P. aeruginosa isolates collected at the University General Hospital of Patras (UGHP) in two time periods were investigated.Two groups of P. aeruginosa clinical isolates were compared collected with one year interval, in the UGHP. Group A consisted of 120 isolates recovered during one year (2004), whereas, group B included 240 recovered during a two year period (2006-2007). P. aeruginosa isolates were

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M. Ismail, Yasser, Sahar M. Fayed, Fatma M. Elesawy, Nora Z Abd El-Halim, and Ola S. El-Shimi. "Phenotypic and Molecular Characteristics of Pseudomonas Aeruginosa Isolated from Burn Unit." EJMM-Volume 30-Issue 1 30, no. 1 (2021): 19–28. http://dx.doi.org/10.51429/ejmm30103.

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Background: The biggest concern for a burn team is a nosocomial infection in burn patients, which is a significant health issue. Pseudomonas aeruginosa is an extremely troublesome drug-resistant bacterium in the world today. We are now faced with rising P. aeruginosa pan-drug-resistant clones in hospital settings. Objectives: To evaluate the distribution of different virulence factors generated by P. aeruginosa isolated from burn wound infections, together with its antimicrobial susceptibility. Methodology: The isolates reported as P. aeruginosa were further tested for the presence of various

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Bahniuk, N., M. Faustova, K. Riesbeck, et al. "THE CORRESPONDENCE OF THE CARBAPENEMASE GENOTYPE AND PHENOTYPIC ANTIMICROBIAL PROFILES OF PSEUDOMONAS AERUGINOSA." Medical and Ecological Problems 27, no. 5-6 (2023): 45–50. http://dx.doi.org/10.31718/mep.2023.27.5-6.06.

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The aim of the study was to determine the correspondence between the carbapenemase genotype and the phenotypic antimicrobial profiles of P. aeruginosa. Materials and methods. The study included 51 clinical isolates of P. aeruginosa, isolated from the patients with post-operative complications of the respiratory organs. The final identification of the obtained isolates was performed in the Riesbeck laboratory using MALDI-ToF (Bruker), followed by the determination of their sensitivity to antimicrobial drugs at the EUCAST Development Laboratory (Växjö, Sweden). Determination of the resistance ge

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Bobai, Mathew, Lawal Danjuma, Nura Muhammad Sani, Joshua Istifanus Anekoson, and Yusuf Nuhu. "Correlation Between Patients with Wounds and Isolated Pseudomonas aeruginosa and Staphylococcus aureus at Barau Dikko Teaching Hospital, Kaduna, Nigeria." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 2 (2022): 35–41. http://dx.doi.org/10.54987/jobimb.v10i2.755.

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This study was carried out to determine the correlation between patients with wounds and isolated Pseudomonas aeruginosa and Staphylococcus aureus in clinics at Barau Dikko Teaching Hospital, Kaduna, Nigeria. The Socio-demographic, medical and drug histories and characteristics of the wounds from each consented patient were taken using a questionnaire along with sixty samples of the patient’s wound swab samples. Isolation of Pseudomonas aeruginosa and Staphylococcus aureus from the wound swab samples was carried out using standard phenotypic and genotypic procedures. Out of the 60 samples coll

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McMullen, Allison R., Melanie L. Yarbrough, Meghan A. Wallace, Angela Shupe, and Carey-Ann D. Burnham. "Evaluation of Genotypic and Phenotypic Methods to Detect Carbapenemase Production in Gram-Negative Bacilli." Clinical Chemistry 63, no. 3 (2017): 723–30. http://dx.doi.org/10.1373/clinchem.2016.264804.

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Abstract BACKGROUND Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS We examined 189 carbapenem-resistant GNB(CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation me

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Borisova, Dayana, Tanya Strateva, Svetoslav G. Dimov, et al. "Diversification of Pseudomonas aeruginosa After Inhaled Tobramycin Therapy of Cystic Fibrosis Patients: Genotypic and Phenotypic Characteristics of Paired Pre- and Post-Treatment Isolates." Microorganisms 13, no. 4 (2025): 730. https://doi.org/10.3390/microorganisms13040730.

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This study examines the impact of inhaled tobramycin therapy on the within-host changes in P. aeruginosa strains isolated from Bulgarian patients with CF prior to and post treatment. Genotypic comparison by RAPD-PCR indicated that most of the pre-treatment isolates had a high similarity and were genetically comparatively close to strains from other countries with known increased morbidity or treatment requirements. Most of the post-treatment isolates were, however, genetically distant from their pre-treatment counterparts, showing genotypic diversification after the treatment. Phenotypic compa

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Lukyanenko, N. V., K. I. Sursyakova, T. V. Safyanova, V. V. Prokopyev, and A. A. Russkikh. "Comparative Aspects of Phenotypic and Genotypic Characteristics of Strains of ISMP Pathogens Isolated from Patients of a Large Multidisciplinary Hospital." Medicina 13, no. 1 (2025): 33–46. https://doi.org/10.29234/2308-9113-2024-13-1-33-46.

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Introduction: In recent decades, Russia, like the rest of the world, has seen a rapid increase in antimicrobial resistance (AMR) among pathogens causing infectious diseases. The development of drug resistance leads to microorganisms' ability to survive despite the use of etiotropic therapy. Alongside multidrug resistance (MDR), hypervirulence – associated with the acquisition of additional genetic material and the emergence of new genetic lineages – is becoming increasingly significant. The purpose of the study: To conduct a comparative phenotypic and genotypic assessment of the main pathogens

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Dave, Alpana, Apurwa Samarth, Roshni Karolia, et al. "Characterization of Ocular Clinical Isolates of Pseudomonas aeruginosa from Non-Contact Lens Related Keratitis Patients from South India." Microorganisms 8, no. 2 (2020): 260. http://dx.doi.org/10.3390/microorganisms8020260.

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P. aeruginosa is the most common Gram-negative organism causing bacterial keratitis. Pseudomonas utilizes various virulence mechanisms to adhere and colonize in the host tissue. In the present study, we examined virulence factors associated with thirty-four clinical P. aeruginosa isolates collected from keratitis patients seeking care at L V Prasad Eye Institute, Hyderabad. The virulence-associated genes in all the isolates were genotyped and characteristics such as antibiotic susceptibility, biofilm formation, swarming motility, pyoverdine production and cell cytotoxicity were analyzed. All t

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Milojković, Marko, Željka Nenadović, Slaviša Stanković, et al. "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia." Archives of Industrial Hygiene and Toxicology 71, no. 3 (2020): 231–50. http://dx.doi.org/10.2478/aiht-2020-71-3418.

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AbstractDrug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of iso

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Barbosa, Thaís Alves, Maria Regina Bentlin, Lígia Maria Suppo de Souza Rugolo, et al. "Molecular Characterization of Gram-Negative Bacilli Isolated from a Neonatal Intensive Care Unit and Phenotypic and Molecular Detection of ESBL and Carbapenemase." Antibiotics 14, no. 4 (2025): 342. https://doi.org/10.3390/antibiotics14040342.

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Introduction: The increase in the rates of multidrug-resistant bacteria in healthcare environments has been recognized as a global public health problem. In view of the scarcity of data on the neonatal population, this study aimed to provide information on the genotypic and epidemiological characteristics of Gram-negative microorganisms isolated from colonization and infection sites in neonates admitted to a tertiary university center of high complexity. Methods: Enterobacterales and non-fermenting Gram-negative bacilli previously collected in a prospective cohort study were submitted to genot

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Gill, Christian M., Tomefa E. Asempa, and David P. Nicolau. "656. Development and Application of a Pragmatic Algorithm for the Detection of Carbapenemase-Producing Pseudomonas aeruginosa (CP-PA)." Open Forum Infectious Diseases 7, Supplement_1 (2020): S384—S385. http://dx.doi.org/10.1093/ofid/ofaa439.849.

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Abstract Background Historically, carbapenem-resistance in P. aeruginosa (PA) has been mediated by inducible AmpC, drug efflux, and porin loss; however, carbapenemase production is an increasingly recognized entity. Of these mechanisms, carbapenemases can drastically reduce treatment options and rapidly disseminate. Since broad applications of phenotypic (mCIM/eCIM) and PCR-based detection can be labor intensive and costly, we developed an MIC derived algorithm to streamline use of these definitive carbapenemase detection methodologies. Methods To develop the testing criteria, a challenge set

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DANJUMA, LAWAL, MUSLIM ISMAIL, and NURA MUHAMMAD SANI. "SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED FROM Ocimum gratissimum LEAF AGAINST SOME ANTIBIOTIC-RESISTANT CLINICAL ISOLATES." AFRICAN JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT 16, no. 3 (2024): 48–63. https://doi.org/10.59493/ajopred/2024.3.7.

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The rise of antibiotic resistance necessitates exploring alternative antimicrobial agents. Nanomedicine offers promising solutions, by enhancing the antimicrobial potencies of the plant extracts. The present research aimed at synthesizing, characterizing and determining the antibacterial activity of O. gratissimum leaf synthesized Silver Nanoparticles (AgNPs) against some antibiotic-resistant clinical isolates. The test bacteria were collected from Dutse General Hospital and Rasheed Shekoni Teaching Hospital and confirmed using standard phenotypic and genotypic techniques. The AgNPs were prepa

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Köse Çetinkaya, Aslıhan, Deniz Doğru, Güzin Cinel, et al. "Clinical and laboratory findings of patients with cystic fibrosis: a single center experience from Türkiye." Turkish Journal of Pediatric Disease 19, no. 3 (2025): 92–98. https://doi.org/10.12956/tchd.1562280.

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Objective: The objective of this study was to evaluate the clinical characteristics of patients with cystic fibrosis (CF) and to determine whether there is a relationship between nutritional status and pulmonary functions. Additionally, the study aimed to investigate the relationship between the CFTR genotype and the occurrence of cystic fibrosis-related diabetes (CFRD), as well as the impact of CFTR mutations on the severity of CF pulmonary disease. Material and Methods: The data of 300 CF patients were retrospectively analyzed. Clinical and laboratory characteristics were obtained from unit

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Garza-González, Elvira, Paola Bocanegra-Ibarias, Miriam Bobadilla-del-Valle, et al. "Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network." PLOS ONE 16, no. 3 (2021): e0248614. http://dx.doi.org/10.1371/journal.pone.0248614.

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Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the W

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Girolamini, Luna, Ada Dormi, Tiziana Pellati, et al. "Advances in Legionella Control by a New Formulation of Hydrogen Peroxide and Silver Salts in a Hospital Hot Water Network." Pathogens 8, no. 4 (2019): 209. http://dx.doi.org/10.3390/pathogens8040209.

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Legionella surveillance is an important issue in public health, linked to the severity of disease and the difficulty associated with eradicating this bacterium from the water environment. Different treatments are suggested to reduce Legionella risk, however long-term studies of their efficiency are lacking. This study focused on the activity of a new formulation of hydrogen peroxide and silver salts, WTP828, in the hospital hot water network (HWN) to contain Legionella contamination during two years of treatment. The effectiveness of WTP828 was tested measuring physical-chemical and microbiolo

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Heiba, Samy A. A., Ibthal S. El-Demerdash, and Shimaa E. Rashad. "Evaluation of Biological Control of Sorghum Strains Using Bacillus Thuringiensis and Pseudomonas Aeruginosa Under Drought Stress." Journal of Advanced Zoology 44, no. 4 (2023): 8–22. http://dx.doi.org/10.17762/jaz.v44i4.1321.

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Background: Sorghum is an economically significant staple food crop for more than half a billion people in developing nations, especially in arid and semi-arid locations where drought stress is a significant limiting factor. Despite usually being regarded as tolerant, sorghum suffers severely from drought stress, which lowers its productivity and nutritional quality throughout its principal cultivation areas. Objective: Improvements in DNA fingerprinting by ISSRs, SSRs, and RAPD markers have also been employed in sorghum genetic modification (GMOs) to enhance the economic characteristics of th

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Hamasaeed, Payman Akram. "Resistance to bacterial infection, complication occurring after cardiac surgery." Cellular and Molecular Biology 66, no. 8 (2020): 20–25. http://dx.doi.org/10.14715/cmb/2020.66.8.4.

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To analyze the occurrence of resistant bacterial infection in patients undergoing cardiac surgery hospitalized in the surgical specialty hospital, in Erbil city, Iraq. A prospective study was done on a total of 138 patients operated and hospitalized in an intensive care unit and surgical wards. Bacterial isolates identification was done according to cultural characteristics, microscopic examination, some biochemical tests, analytic Profile Index 20E& API Staph, confirmed with VITEK® 2 compact system (BioMérieux). Antimicrobial susceptibility for disc diffusion tested to 17 antimicrobial ag

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Eswani, Mohamed, Ashraf Naass, Nagib A. Elmarzugi, Ahmed M. Elmarghani, Mohammed Elfgee, and Mohammed O. Elbasir. "APPLICATION OF PHENOTYPIC AND GENOTYPIC STRATEGIES TO IDENTIFY PSEUDOMONAS AERUGINOSA." EPH - International Journal of Applied Science 7, no. 3 (2021): 11–15. http://dx.doi.org/10.53555/eijas.v7i3.52.

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A case study of patient presented with developed wound infection with Pseudomonas aeruginosa (leukocytosis). The strains of P. aeruginosa isolated from Sheffield General Northern Hospital (UK) and studied for phenotypic and genotypic identifications. This present study were conducted in Sheffield Hallam University (UK) and was aimed to evaluate the application of Phenotypic and Genotypic techniques to identify P. aeruginosa. Generally, the results of Real time PCR (RTPCR) revealed that the diagnosis viability was confirmed for P. aeruginosa. However, identification on the basis of phenotype by

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Lizzy C Egwim, Ugochukwu M Okezie, Nancy A Mbachu, and Malachy C Ugwu. "Phenotypic and genotypic detection of antibiotic resistance among Pseudomonas aeruginosa isolates from orthopedic wounds from Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (Coouth), Awka Anambra Nigeria." Magna Scientia Advanced Research and Reviews 9, no. 1 (2023): 044–51. http://dx.doi.org/10.30574/msarr.2023.9.1.0125.

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Pseudomonas aeruginosa is well known orthopedic wounds-associated bacterial pathogen, responsible for prolonged wound treatment, due to its resistance, mediated by different types of extended-spectrum beta-lactamases (ESBL). The present study was undertaken to find the prevalence of extended-spectrum β lactamase (ESBL), Metallo-β lactamase (MBL), and AmpC β lactamase in P. aeruginosa isolated from orthopedic wound samples. Wound swabs were processed and P. aeruginosa was identified by standard protocols. The Kirby-Bauer disc diffusion method was used in ascertaining the antibiotic sensitivity

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Cigana, Cristina, Paola Melotti, Rossella Baldan, et al. "Genotypic and phenotypic relatedness of Pseudomonas aeruginosa isolates among the major cystic fibrosis patient cohort in Italy." BMC Microbiology 16, no. 1 (2016): 142. https://doi.org/10.1186/s12866-016-0760-1.

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<strong>Background: </strong> <i>Pseudomonas aeruginosa</i> is the predominant pathogen associated with the decline of pulmonary function in cystic fibrosis (CF) patients. Both environment-to-host acquisition and patient-to-patient transmission have been described for <i>P. aeruginosa</i> infection. Epidemic clones and bacterial phenotypic adaptation to the CF lung have been recognised as independent risk factors for disease progression. So far, there is no established link between genotypic prevalence and phenotypic traits. Here, we look at the major CF patient cohort in Italy to identify sha

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Islam, Nazrul, Dilruba Ahmed, Nazmul Ahsan, Chowdhury R. Ahsan, and Mahmuda Yasmin. "Phenotypic-genotypic Features of MDR Pseudomonas Aeruginosa and Acinetobacter Baumannii From Dhaka, Bangladesh." Bioresearch Communications 9, no. 2 (2023): 1276–84. http://dx.doi.org/10.3329/brc.v9i2.67079.

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Pseudomonas aeruginosa and Acinetobacter baumannii are one of the most common causes of MBL mediated morbidity and mortality throughout the world. Day-by-day these isolates are showing increasing resistance trends to different antimicrobial agents. But there are few data in Bangladesh, the study was designed to observe the pattern of antimicrobial resistance, prevalence of MBL, AmpC and finally phylogenetic distributions. A total of 200 isolates were analyzed in this study, comprising of 100 MDR-carbapenem resistant P. aeruginosa and 100 MDR-carbapenem resistant A. baumonnii. Isolates were tes

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Aksoy, M. Duygu, and H. Murat Tuğrul. "Investigation of Metallo-Beta-Lactamases in Carbapenem Resistant Pseudomonas aeruginosa Strains by Phenotypic and Genotypic Methods." Flora the Journal of Infectious Diseases and Clinical Microbiology 25, no. 3 (2020): 301–7. http://dx.doi.org/10.5578/flora.68921.

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Introduction: Carbapenem resistant Pseudomonas aeruginosa strains cause serious problems in treatment. A large number of identified metallo-beta-lactamase (MBL) enzymes produced by P. aeruginosa are one of the most important mechanisms in resistance to carbapenems. MBL genes are located on the chromosome or plasmid, and they can easily spread between different bacterial strains. The activities of these enzymes are zinc-dependent, and they are inhibited by ethylenediaminetetraacetic acid (EDTA). Therefore, this advantage is used in MBL identification tests. In this study, it was aimed to determ

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Winstanley, Craig, Stephen B. Kaye, Timothy J. Neal, Helen J. Chilton, Silvia Miksch, and C. Anthony Hart. "Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis." Journal of Medical Microbiology 54, no. 6 (2005): 519–26. http://dx.doi.org/10.1099/jmm.0.46005-0.

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Gholampour Matin, Milad, Reza Shapouri, Mohammadreza Nahaei, Mojtaba Mohammadi Roknabadi, and Rasoul Shokri. "Genotypic Investigation of Antibiotic Resistant blaOXA-4 Gene in Clinical Isolates of Pseudomonas aeruginosa." Avicenna Journal of Clinical Microbiology and Infection 10, no. 3 (2023): 95–99. http://dx.doi.org/10.34172/ajcmi.3471.

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Background: Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is responsible for 10-15% of hospital infections worldwide. The acquisition of resistance genes is one of the important mechanisms that causes the spread of resistance in this bacterium. This study aimed to conduct a phenotypic and genotypic investigation of the blaOXA-4 resistance gene in P. aeruginosa isolated from clinical samples. Methods: In this study, 110 P. aeruginosa strains were isolated from various clinical samples. The disk diffusion method was applied to reveal the resistance pattern in the isolates. Mo

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Upadhyay, Deepa, and Anshu Sharma. "Detection of Metallo-Beta-Lactamase Encoding Genes by Multiplex RT-PCR among Clinical Isolates of Pseudomonas Aeruginosa." International Journal of Health Sciences and Research 14, no. 9 (2024): 256–59. http://dx.doi.org/10.52403/ijhsr.20240933.

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Introduction: Metallo-beta-lactamase producing Pseudomonas aeruginosa is an important emerging nosocomial pathogen, due to its ability to acquired drug resistance. Aim & objectives: The aim of this study was to detect MBL encoding genes by genotypic method among clinical isolates of Pseudomonas aeruginosa. Material & Method: A total of 50 non-repetitive isolates of P. aeruginosa recovered from various clinical samples were included in this study. All these isolates were screened for MBL production by RT-PCR irrespective of imipenem sensitivity and the presence of blaVIM, blaIMP & b

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Pappa, O., G. Mandilara, A. Vatopoulos, and A. Mavridou. "Typing of Pseudomonas aeruginosa strains isolated from Greek water samples by three typing methods: serotyping, Random Amplified Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE)." Water Science and Technology 67, no. 6 (2013): 1380–88. http://dx.doi.org/10.2166/wst.2013.678.

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The study of various types of Pseudomonas aeruginosa strains isolated from water environments is of paramount importance from a public health point of view, due to their ubiquity and pathogenicity. Molecular (Random Amplified Polymorphic DNA and Pulsed Field Gel Electrophoresis) and phenotypical (serotyping) typing methods were applied to environmental P. aeruginosa strains. The typeability and discriminatory power of the methods were studied and compared. The two molecular methods managed to type a number of P. aeruginosa strains which were non-serotypeable due to their rough phenotypes. Acco

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Hamza, Dalia, and Hala M. Zaher. "Carriage of Rifampicin- and Multidrug-Resistant Pseudomonas aeruginosa in Apparently Healthy Camels: A View Through a Zoonosis Lens." Microbiology Research 16, no. 6 (2025): 107. https://doi.org/10.3390/microbiolres16060107.

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Pseudomonas aeruginosa poses a significant global concern in human and veterinary medicine due to its resistance to multiple antimicrobials. Limited research has been carried out on rifampicin-resistant P. aeruginosa, particularly in food-producing animals such as camels. Therefore, the purpose of this study was to investigate the occurrence of rifampicin- and multidrug-resistant P. aeruginosa in apparently healthy camels. Nasal swabs and tissue samples were collected from one hundred apparently healthy slaughtered camels, and they were subjected to bacteriological isolation and identification

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Ventero, Maria Paz, Jose M. Haro-Moreno, Carmen Molina-Pardines, et al. "Role of Relebactam in the Antibiotic Resistance Acquisition in Pseudomonas aeruginosa: In Vitro Study." Antibiotics 12, no. 11 (2023): 1619. http://dx.doi.org/10.3390/antibiotics12111619.

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Background: Pseudomonas aeruginosa shows resistance to several antibiotics and often develops such resistance during patient treatment. Objective: Develop an in vitro model, using clinical isolates of P. aeruginosa, to compare the ability of the imipenem and imipenem/relebactam to generate resistant mutants to imipenem and to other antibiotics. Perform a genotypic analysis to detect how the selective pressure changes their genomes. Methods: The antibiotics resistance was studied by microdilution assays and e-test, and the genotypic study was performed by NGS. Results: The isolates acquired res

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Elshazely, Rowyda, Ibrahim Amer, Salah Aal, and Asmaa Tahoun. "Antibacterial effect of Moringa oleifera on Staphylococcus aureus and Pseudomonas aeruginosa isolated from raw milk and some dairy products with special reference to biofilm gene expression." Open Veterinary Journal 14, (1) (Zagazig Veterinary Confer (2024): 164. http://dx.doi.org/10.5455/ovj.2024.v14.i1.15.

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Background: Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are well defined as food poisoning pathogens which are highly resistant and need continuous studies. Aim: The purpose of the work was to examine phenotypic and genotypic characters of both P. aeruginosa and S. aureus, and treatment trial with medicinal plant. Methods: Samples were examined for isolation of P. aeruginosa and S. aureus on selective media followed by biochemical confirmation, biofilm formation, genes detection, and expression of P. aeruginosa pslA biofilm gene was performed by qRT-PCR after t

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Pourmohammadi, Naser, Reza Shapouri, Mohammadreza Nahaei, and Mojtaba Mohammadi Roknabadi. "Genotypic Investigation of Antibiotic Resistance: Presence of BlaOXA-48 Gene in Clinical Isolates of Pseudomonas aeruginosa." Avicenna Journal of Clinical Microbiology and Infection 11, no. 2 (2024): 62–67. http://dx.doi.org/10.34172/ajcmi.3508.

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Background: Identifying antimicrobial resistance patterns and antibiotic resistance genes in clinical isolates of Pseudomonas aeruginosa is an important strategy for effectively combating infections. This study aimed to identify the antimicrobial resistance patterns and the presence of blaOXA-48 beta-lactamase gene in P. aeruginosa isolated from clinical samples. Methods: We collected 100 isolates of P. aeruginosa from different clinical samples, including urine, tracheal tube, blood, and burn wounds. The antimicrobial resistance patterns of the isolates were investigated using the disk diffus

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A. Ali, Aysar, and Enass G. Sweedan. "Role of higB-higA Novel Genes in Antibiotics Resistance of Pseudomonas aeruginosa." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 13, no. 02 (2022): 605–9. http://dx.doi.org/10.25258/ijddt.13.2.22.

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Background: Pseudomonas aeruginosa is a devious pathogen with the tendency to prompt many acute and serious chronic diseases. This study aims to detect novel genes (Toxins-Antitoxins II system), especially; higB and higA encoded from P. aeruginosa by PCR technique and the relation between these genes and antibiotic resistance of P. aeruginosa. Methods: This study detected 50 isolates of P. aeruginosa from distinct clinical sources. The most common origin of isolates was (44%) burn swabs, (22%) urine culture, (12%) wound swabs, (14%) sputum, and (8%) ear swabs. The bacteria were isolated using

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Díaz-Ríos, Catalina, Marta Hernández, David Abad, et al. "New Sequence Type ST3449 in Multidrug-Resistant Pseudomonas aeruginosa Isolates from a Cystic Fibrosis Patient." Antibiotics 10, no. 5 (2021): 491. http://dx.doi.org/10.3390/antibiotics10050491.

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Pseudomonas aeruginosa is one of the most critical bacterial pathogens associated with chronic infections in cystic fibrosis patients. Here we show the phenotypic and genotypic characterization of five consecutive multidrug-resistant isolates of P. aeruginosa collected during a month from a CF patient with end-stage lung disease and fatal outcome. The isolates exhibited distinct colony morphologies and pigmentation and differences in their capacity to produce biofilm and virulence potential evaluated in larvae of Galleria mellonella. Whole genome-sequencing showed that isolates belonged to a n

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Abaza, Amani F., Soraya A. El Shazly, Heba S. A. Selim, and Gehan S. A. Aly. "Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt." Polish Journal of Microbiology 66, no. 3 (2017): 297–308. http://dx.doi.org/10.5604/01.3001.0010.4855.

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Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. This cross sectional study included 1583 clinical samples that were collected from patients admitted to Alexandria University Students’ Hospital. P. aeruginosa isolates were identified using standard microbiological methods and were tested for their antimicrobial susceptibility pat

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Al-Salami, Mohammed Abbas Farman, and Nabil Salim Saaid Tuwaij. "Genotypic investigation of drugs resistance and biofilm genes among ciprofloxacin-resistant Pseudomonas aeruginosa isolated from burn patients." BIO Web of Conferences 84 (2024): 03015. http://dx.doi.org/10.1051/bioconf/20248403015.

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Burn patients are the serious targets of hospital-acquired infection occurred by Pseudomonas aeruginosa ( P. aeruginosa) , which is a main cause of burn patients morbidity and mortality, so this study aimed to molecular investigation of genes associated with antibiotic resistance among ciprofloxacin-resistant Pseudomonas aeruginosa. Current study involved 168 burn patients involved 107(63.69%) and 61 (36.3%) were females and male respectively, the results showed 132(78.57%) gram negative bacteria and 27(16.0%) gram positive bacteria while 9(5.3%) no bacterial growth. The results of Vitek-2 com

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Othman, Hemin E., Eric L. Miller, Jaladet Ms Jubrael, and Ian S. Roberts. "Genotypic characterization and novel multilocus sequence types of exoU+ Pseudomonas aeruginosa from different clinical infections and environments." Innovaciencia Facultad de Ciencias Exactas, Físicas y Naturales 6, no. 1 (2018): 1–14. http://dx.doi.org/10.15649/2346075x.458.

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Introduction: The exoU gene, a marker for highly virulent strains of Pseudomonas aeruginosa, is the major contributor to a wide variety of healthcare-associated infections. Methods: In this study, the antibiotic susceptibility profile, prevalence and genotyping of exoU+ P. aeruginosa were demonstrated. A total of 101 isolates of P. aeruginosa were analysed from different clinical and environmental sources. Results: The antibiotic susceptibility profile classified these isolates as extensively drug resistant (35.6%), multidrug resistant (40.5%) and non-multidrug resistant (23.7%). The prevalenc

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Balko, O. B., L. B. Zelena, O. I. Balko, N. A. Bobyr, V. G. Voitsekhovsky, and L. V. Avdeeva. "Phenotypic and Genotypic Criteria for the Screening of Highly Active S-Type Pyocins Pseudomonas aeruginosa Producers." Mikrobiolohichnyi Zhurnal 86, no. 1 (2024): 39–50. http://dx.doi.org/10.15407/microbiolj86.01.039.

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Bacteriocins of Pseudomonas aeruginosa, especially S-type pyocins, show high efficiency as analogs of antimicrobial drugs. Various screening methods can be used to identify producers of highly active pyocins, but there are no clear criteria for selecting perspective strains. The aim of this work was to determine criteria that can be used during phenotypic and genotypic screening for the selection of perspective highly active S-type pyocin P. aeruginosa producers. Methods. The objects of investigation were 40 P. aeruginosa strains. Pyocins were obtained from each culture, relative coefficients

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Saputra, I. Wayan Agus Gede Manik, Ni Made Mertaniasih, and Ni Nengah Dwi Fatmawati. "Positivity of ExoU Gene of Type III Secretion System and Fluoroquinolone Resistance of Psedomonas aeruginosa from Sputum of Nosocomial Pneumonia Patients in Sanglah Hospital, Bali." Folia Medica Indonesiana 54, no. 2 (2018): 129. http://dx.doi.org/10.20473/fmi.v54i2.8863.

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Pseudomonas aeruginosa is one of the Gram-negative rods bacteria that frequently cause nosocomial pneumonia. One of the main virulent effector proteins on Type III secretion system (TTSS) of P. aeruginosa is Exoenzyme U ( ExoU). ExoU works as a phospholipase A2 activity and exhibits lung tissue injury effect in pneumonia. As an antibiotic that has activity against P. aeruginosa, fluoroquinolone resistance has increased as many as three fold since the last decade. Infections caused by P. aeruginosa that are fluoroquinolone resistant and positive for ExoU gene show worse clinical outcome. The ai

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Chilam, Jeremiah, Silvia Argimon, Marilyn Limas, et al. "Genomic surveillance of Pseudomonas aeruginosa in the Philippines, 2013–2014." Western Pacific Surveillance and Response Journal 12, no. 2 (2021): 4–18. http://dx.doi.org/10.5365/wpsar.2020.11.1.006.

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Pseudomonas aeruginosa is an opportunistic pathogen that often causes nosocomial infections resistant to treatment. Rates of antimicrobial resistance (AMR) are increasing, as are rates of multidrug-resistant (MDR) and possible extensively drug-resistant (XDR) infections. Our objective was to characterize the molecular epidemiology and AMR mechanisms of this pathogen. We sequenced the whole genome for each of 176 P. aeruginosa isolates collected in the Philippines in 2013–2014; derived the multilocus sequence type (MLST), presence of AMR determinants and relatedness between isolates; and determ

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Putignani, L., R. Sessa, A. Petrucca, et al. "Genotyping of Different Pseudomonas Aeruginosa Morphotypes Arising from the Lower Respiratory Tract of a Patient Taken to an Intensive Care Unit." International Journal of Immunopathology and Pharmacology 21, no. 4 (2008): 941–47. http://dx.doi.org/10.1177/039463200802100418.

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Pseudomonas aeruginosa is an opportunistic pathogen and an ubiquitous environmental bacterium. Fifty-seven days after hospitalization, we isolated three distinct P. aeruginosa morphotypes (smooth, rough and mucoid) from the lower respiratory tract of a patient admitted to a Cardiology Intensive Care Unit (ICU). Moreover, a group of nine colony variants, arising from the three P. aeruginosa isolates growing in laboratory growth media, were also isolated. The resulting 12 isolates were characterised for antibiotic resistance profile and subjected to genotypic analysis by fluorescent-Amplified Fr

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Winstanley, C., J. L. Fothergill, S. Panagea, C. H. M. Smart, M. J. Walshaw, and C. A. Hart. "71 Phenotypic and genotypic characteristics of a Cystic Fibrosis epidemic strain of Pseudomonas aeruginosa." Journal of Cystic Fibrosis 5 (2006): S16. http://dx.doi.org/10.1016/s1569-1993(06)80062-7.

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Highsmith, Anita K., Phuong Nhan Le, Rima F. Khabbaz, and Van P. Munn. "Characteristics of Pseudomonas aeruginosa Isolated From Whirlpools and Bathers." Infection Control 6, no. 10 (1985): 407–12. http://dx.doi.org/10.1017/s0195941700063499.

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AbstractPseudomonas aeruginosa is the most frequently isolated microorganism from whirlpool water and lesions associated with outbreaks of dermatitis and folliculitis related to whirlpool exposure. Strains were selected from 19 outbreaks of P. aeruginosa infections (1977 to 1983) associated with whirlpool use; they were examined to determine if the strains possessed unique virulence factors or characteristics that might aid in their selection in the environment.P. aeruginosa, 011, was the predominant serotype isolated from whirlpool water as well as from bathers with dermatitis or folliculitis

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Abdelatti, Mohamed A. I., Norhan K. Abd El-Aziz, El-sayed Y. M. El-Naenaeey, et al. "Antibacterial and Anti-Efflux Activities of Cinnamon Essential Oil against Pan and Extensive Drug-Resistant Pseudomonas aeruginosa Isolated from Human and Animal Sources." Antibiotics 12, no. 10 (2023): 1514. http://dx.doi.org/10.3390/antibiotics12101514.

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Pseudomonas aeruginosa is notorious for its ability to develop a high level of resistance to antimicrobial agents. Resistance-nodulation-division (RND) efflux pumps could mediate drug resistance in P. aeruginosa. The present study aimed to evaluate the antibacterial and anti-efflux activities of cinnamon essential oil either alone or combined with ciprofloxacin against drug resistant P. aeruginosa originated from human and animal sources. The results revealed that 73.91% of the examined samples were positive for P. aeruginosa; among them, 77.78% were of human source and 72.73% were recovered f

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Pajaro-Castro, Nerlis, Erick Diaz-Morales, Kenia Hoyos, and Cristhian Ibañez-Bersinger. "Whole-Genome Sequencing of Resistance, Virulence and Regulation Genes in Extremely Resistant Strains of Pseudomonas aeruginosa." Medical Sciences 13, no. 1 (2025): 6. https://doi.org/10.3390/medsci13010006.

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Background/Objectives: Pseudomonas aeruginosa is a clinically significant opportunistic pathogen, renowned for its ability to acquire and develop diverse mechanisms of antibiotic resistance. This study examines the resistance, virulence, and regulatory mechanisms in extensively drug-resistant clinical strains of P. aeruginosa. Methods: Antibiotic susceptibility was assessed using the Minimum Inhibitory Concentration (MIC) method, and whole-genome sequencing (WGS) was performed on the Illumina NovaSeq platform. Results: The analysis demonstrated a higher prevalence of virulence genes compared t

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Tyumentseva, Marina, Yulia Mikhaylova, Anna Prelovskaya, et al. "CRISPR Element Patterns vs. Pathoadaptability of Clinical Pseudomonas aeruginosa Isolates from a Medical Center in Moscow, Russia." Antibiotics 10, no. 11 (2021): 1301. http://dx.doi.org/10.3390/antibiotics10111301.

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Pseudomonas aeruginosa is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical P. aeruginosa isolates based on whole-genome sequencing data. The isolate collection studied was characterized by a variety of clonal lineages with a domination of high-risk epidemic clones and different CRISPR/Cas element patterns. This is the first report on the coexistence of

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Tadjrobehkar, Omid, and Atefeh Kamali. "Evaluation of Antibiotic Resistance Pattern and Extended-Spectrum Beta-lactamases in Pseudomonas aeruginosa Isolates Obtained from Clinical Samples by Phenotypic and Genotypic Methods in Zabol, Iran." Journal of Kerman University of Medical Sciences 29, no. 6 (2022): 529–35. http://dx.doi.org/10.34172/jkmu.2022.64.

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Background: Pseudomonas aeruginosa is a human opportunistic pathogen that is known to be responsible for various diseases. However, its antibiotic-resistant isolates often cause serious infections. Methods: This study for the first time investigated a total of 80 P. aeruginosa isolates collected from patients admitted to Amir Al-Momenin hospital. The isolates were identified by biochemical assays. The combination disc test method was used to measure antibiotic susceptibility and confirm the presence of extended spectrum-beta lactamases-producing enzymes. Also, the presence of enzyme-producing

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Matroș, Luminița, Tibor Ludovic Krausz, Stanca Lucia Pandrea, et al. "Phenotypic and genotypic study of carbapenem-resistant Pseudomonas aeruginosa strains isolated from hospitalized patients." Revista Romana de Medicina de Laborator 24, no. 2 (2016): 201–11. http://dx.doi.org/10.1515/rrlm-2016-0021.

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Abstract Introduction: Nosocomial infections caused by Pseudomonas aeruginosa producing carbapenemases represent an important cause of morbidity and mortality among immunosuppressed patients. The aim of our study was to detect the production of metallo-carbapenemases (MBLs) by phenotypic methods and to detect the presence of the MBLs encoding genes (blaIMP and blaVIM) by PCR in P. aeruginosa strains isolated from hospitalized patients to the Regional Institute of Gastroenterology and Hepatology, Cluj-Napoca. Material and methods: Between September 2014-February 2015, we tested thirty-eight P.

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, and Gopal Nayak. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Omics Publishing Group, October 7, 2015. https://doi.org/10.5281/zenodo.813958.

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Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi's biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using a

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Iwańska, Agnieszka, Elżbieta Anna Trafny, Michał Czopowicz, and Ewa Augustynowicz-Kopeć. "Phenotypic and genotypic characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients with chronic infections." Scientific Reports 13, no. 1 (2023). http://dx.doi.org/10.1038/s41598-023-39005-9.

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AbstractPatients with cystic fibrosis are predisposed to chronic respiratory tract infections caused by Pseudomonas aeruginosa. As the disease progresses, the microorganism diversifies into genotypically and phenotypically different strains which may coexist in the patient's airways for years. Adaptation of the microorganism to the airways of patients with cystic fibrosis probably occurs in response to the host's airway environment, the elements of the immune system and antibiotic therapy. Due to the chronic persistence of the microorganism in the airways, a comprehensive molecular analysis wa

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Journal of Tropical Diseases 4, no. 1 (2015). https://doi.org/10.5281/zenodo.166324.

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<strong>Introduction:</strong> Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. <strong>Methods:</strong> P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemic

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