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Journal articles on the topic 'P. aeruginosa'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Gasink, Leanne B., Neil O. Fishman, Irving Nachamkin, Warren B. Bilker, and Ebbing Lautenbach. "Risk Factors for and Impact of Infection or Colonization With Aztreonam-Resistant Pseudomonas aeruginosa." Infection Control & Hospital Epidemiology 28, no. 10 (2007): 1175–80. http://dx.doi.org/10.1086/520740.

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Objective.To identify risk factors for infection or colonization with aztreonam-resistant Pseudomonas aeruginosa and examine the impact of this organism on mortality.Design.A case-control study was performed to identify risk factors for infection or colonization with aztreonam-resistant P. aeruginosa. A cohort study was subsequently performed to examine the impact of aztreonam resistance on outcomes.Setting.A tertiary referral center in southeastern Pennsylvania.Participants.Inpatients with a clinical culture positive for P. aeruginosa between January 1, 1999, and December 31, 2000.Results.Of

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Aguilera-Herce, Julia, Meritxell García-Quintanilla, Rocío Romero-Flores, Michael J. McConnell, and Francisco Ramos-Morales. "A Live Salmonella Vaccine Delivering PcrV through the Type III Secretion System Protects against Pseudomonas aeruginosa." mSphere 4, no. 2 (2019): e00116-19. https://doi.org/10.1128/mSphere.00116-19.

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<em>Pseudomonas aeruginosa</em> is a common Gram-negative opportunistic pathogen that is intrinsically resistant to a wide range of antibiotics. The development of a broadly protective vaccine against <em>P. aeruginosa</em> remains a major challenge. Here, we used an attenuated strain of <em>Salmonella enterica</em> serovar Typhimurium as a vehicle to express <em>P. aeruginosa</em> antigens. A fusion between the <em>S. enterica</em> type III secretion effector protein SseJ and the <em>P. aeruginosa</em> antigen PcrV expressed under the control of the <em>sseA</em> promoter was translocated by

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3

Ahmed, Faraz, Zulfiqar Ali Mirani, Ayaz Ahmed, et al. "Nanotubes Formation in P. aeruginosa." Cells 11, no. 21 (2022): 3374. http://dx.doi.org/10.3390/cells11213374.

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The present study discusses a biofilm-positive P. aeruginosa isolate that survives at pH levels ranging from 4.0 to 9.0. The biofilm consortia were colonized with different phenotypes i.e., planktonic, slow-growing and metabolically inactive small colony variants (SCVs). The lower base of the consortia was occupied by SCVs. These cells were strongly attached to solid surfaces and interconnected through a network of nanotubes. Nanotubes were observed at the stationary phase of biofilm indwellers and were more prominent after applying weight to the consortia. The scanning electron micrographs in

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4

Mohmed, Al-shahrani, Elhedmi Abdulkhaleg, Elkaib Hossam, Jrad Samira, and Alnaqib Shaymaa. "Determination and Study of Main Biological Features of Bacteriophages Active Against Bacteria Pseudomonas fluorescens and Pseudomonas aeruginosa." Alq J Med App Sci 6, no. 2 (2023): 507–10. https://doi.org/10.5281/zenodo.8280466.

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<strong>Background and aims</strong>. One of the significant areas of modern microbiology and biotechnology is the study of bacteriophages. This is due to the growing interest in terms of their practical application in various branches of medicine, agriculture, and the food industry. The purpose of our work was to isolate bacteriophages active against bacteria of the genus Pseudomonas and to study their biological properties. <strong>Methods</strong>. Bacteriophages isolated from spoiled poultry, beef and carp samples were the subjects of the study. 20 g of the weighed sample were inoculated w

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5

Worgall, Stefan, Toshiaki Kikuchi, Ravi Singh, Katherine Martushova, Leah Lande, and Ronald G. Crystal. "Protection against Pulmonary Infection with Pseudomonas aeruginosa following Immunization with P. aeruginosa-Pulsed Dendritic Cells." Infection and Immunity 69, no. 7 (2001): 4521–27. http://dx.doi.org/10.1128/iai.69.7.4521-4527.2001.

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ABSTRACT To develop a Pseudomonas aeruginosa vaccine that allows the host immune system to select the antigens, we hypothesized that dendritic cells (DC) pulsed with P. aeruginosa would induce protective immunity against pulmonary infections with P. aeruginosa. Incubation of murine bone marrow-derived DC with P. aeruginosa in vitro led to uptake of P. aeruginosa and activation of the DC. Spleen-derived CD4+ cells from mice immunized withP. aeruginosa-pulsed DC showed increased proliferation, demonstrating that DC pulsed with P. aeruginosa were capable of eliciting a P. aeruginosa-specific immu

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6

De Muynck, Benedicte, Anke Van Herck, Annelore Sacreas, et al. "Successful Pseudomonas aeruginosa eradication improves outcomes after lung transplantation: a retrospective cohort analysis." European Respiratory Journal 56, no. 4 (2020): 2001720. http://dx.doi.org/10.1183/13993003.01720-2020.

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Long-term survival after lung transplantation (LTx) is hampered by development of chronic lung allograft dysfunction (CLAD). Pseudomonas aeruginosa is an established risk factor for CLAD. Therefore, we investigated the effect of P. aeruginosa eradication on CLAD-free and graft survival.Patients who underwent first LTx between July, 1991, and February, 2016, and were free from CLAD, were retrospectively classified according to P. aeruginosa presence in respiratory samples between September, 2011, and September, 2016. P. aeruginosa-positive patients were subsequently stratified according to succ

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7

Aidah Abd Al-doori, Awatef Saber Jasem, and Adnan F. AL-Azzawie. "Effects of Nd:Yag Laser on some virulence factor genes of Pseudomonas aeruginosa bacteria." Tikrit Journal of Pure Science 25, no. 2 (2020): 86–92. http://dx.doi.org/10.25130/tjps.v25i2.240.

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The aim of this study was to assess effects of the 532nm Nd-yag laser on the genes of Tox A, Exo S, and Opr L, of Pseudomonas aeruginosa (P. aeruginosa) bacteria isolated from clinical (wounds, burns, otitis media) and environmental (water, soil) samples. Clinical samples were collected from patients coming to Saladdin General Hospital from wound, burns and middle ear infections while environmental samples were extracted from water and soil for Saladdin General Hospital . Bacterial samples irradiated by Nd-Yag laser with wavelength of 532 nm using energies (300mj,500mj) with (15 and 25 sec) an

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8

Chakraborty, Tamalika, Tushar Gupta, Nayana Verma, Zarin Parwez, Kaustav Deb, and Tamoghana Chakraborty. "PREVALENCE OF ANTIBIOTIC RESISTANCE IN PSEUDOMONAS AERUGINOSA." International Journal of Advanced Research 12, no. 01 (2024): 1249–60. http://dx.doi.org/10.21474/ijar01/18243.

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One of the main bacteria responsible for hospital-acquired illnesses is Pseudomonas aeruginosa. Through chromosomal changes or the horizontal acquisition of resistant determinants, antibiotic resistance can be easily developed it. High-risk clones, like ST175, are spreading together with the rising frequency of extensively-drug-resistant (XDR) or multi-drug-resistant (MDR) P. aeruginosa isolates. MDR/XDR infections should be taken seriously since they can make it difficult to choose the best empirical and conclusive antimicrobial therapies. New avenues for the treatment of MDR/XDR P. aeruginos

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9

Al-Hashimi, Omar, Ibrahim Omar Saeed, and Safaa Abed Lateef Al Meani. "Evaluating the qualitative characteristics and heavy elements of hospital water and their relationship with bioresistance in P. aeruginosa." Technium BioChemMed 8 (May 1, 2024): 64–75. http://dx.doi.org/10.47577/biochemmed.v8i.10934.

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ABSTRACT: Bacteria use the elements present in the environment to develop their vital defenses, trying to acquire genes from other strains or absorb heavy metals in order to adapt to them and increase their tolerance against high concentrations of heavy elements. Pseudomonas aeruginosa is an opportunistic bacterial pathogen that causes infections in hospitals and communities, including in humans and animals. P. aeruginosa's adaptability and endurance in therapeutic settings are cause for concern. Emerging pathogenic strains pose a global threat and cause significant concern. Biocides are commo

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10

Higgins, Gerard, Coral Fustero Torre, Jean Tyrrell, Paul McNally, Brian J. Harvey, and Valerie Urbach. "Lipoxin A4prevents tight junction disruption and delays the colonization of cystic fibrosis bronchial epithelial cells byPseudomonas aeruginosa." American Journal of Physiology-Lung Cellular and Molecular Physiology 310, no. 11 (2016): L1053—L1061. http://dx.doi.org/10.1152/ajplung.00368.2015.

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The specialized proresolution lipid mediator lipoxin A4(LXA4) is abnormally produced in cystic fibrosis (CF) airways. LXA4increases the CF airway surface liquid height and stimulates airway epithelial repair and tight junction formation. We report here a protective effect of LXA4(1 nM) against tight junction disruption caused by Pseudomonas aeruginosa bacterial challenge together with a delaying action against bacterial invasion in CF airway epithelial cells from patients with CF and immortalized cell lines. Bacterial invasion and tight junction integrity were measured by gentamicin exclusion

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11

Harrigan, James, Ebbing Lautenbach, Emily Reesey, et al. "Impact of Diagnosed and Undiagnosed Respiratory Pseudomonas on VAP and VAE During Long-Term Acute Care." Infection Control & Hospital Epidemiology 41, S1 (2020): s258—s259. http://dx.doi.org/10.1017/ice.2020.823.

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Background: Clinically diagnosed ventilator-associated pneumonia (VAP) is common in the long-term acute-care hospital (LTACH) setting and may contribute to adverse ventilator-associated events (VAEs). Pseudomonas aeruginosa is a common causative organism of VAP. We evaluated the impact of respiratory P. aeruginosa colonization and bacterial community dominance, both diagnosed and undiagnosed, on subsequent P. aeruginosa VAP and VAE events during long-term acute care. Methods: We enrolled 83 patients on LTACH admission for ventilator weaning, performed longitudinal sampling of endotracheal aspi

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Gonzaga, Zennia Jean C., Christina Merakou, Antonio DiGiandomenico, Gregory P. Priebe, and Bernd H. A. Rehm. "A Pseudomonas aeruginosa-Derived Particulate Vaccine Protects against P. aeruginosa Infection." Vaccines 9, no. 7 (2021): 803. http://dx.doi.org/10.3390/vaccines9070803.

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Despite numerous efforts to develop an effective vaccine against Pseudomonas aeruginosa, no vaccine has yet been approved for human use. This study investigates the utility of the P. aeruginosa inherently produced polyhydroxyalkanaote (PHA) inclusions and associated host–cell proteins (HCP) as a particulate vaccine platform. We further engineered PHA inclusions to display epitopes derived from the outer membrane proteins OprF/OprI/AlgE (Ag) or the type III secretion system translocator PopB. PHA and engineered PHA beads induced antigen-specific humoral, cell-mediated immune responses, anti-HCP

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13

Kaye, Keith S., Zeina A. Kanafani, Ashley E. Dodds, John J. Engemann, Stephen G. Weber, and Yehuda Carmeli. "Differential Effects of Levofloxacin and Ciprofloxacin on the Risk for Isolation of Quinolone-Resistant Pseudomonas aeruginosa." Antimicrobial Agents and Chemotherapy 50, no. 6 (2006): 2192–96. http://dx.doi.org/10.1128/aac.00060-06.

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ABSTRACT Due to the greater in vitro activity of ciprofloxacin than that of levofloxacin against Pseudomonas aeruginosa, the likelihood of isolating a clinical strain of quinolone-resistant (QR) P. aeruginosa might be greater after exposure to levofloxacin than ciprofloxacin. We examined the risk of isolating QR P. aeruginosa in association with prior levofloxacin or ciprofloxacin exposure. A case-case-control study was conducted. Two groups of cases, one with nosocomial QR P. aeruginosa infections and one with nosocomial quinolone-susceptible (QS) P. aeruginosa infections, were compared to a

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14

Al-doori1, Aidah Abd, Awatef Saber Jasem1, and Adnan F. AL-Azzawie2. "Effects of Nd:Yag Laser on some virulence factor genes of Pseudomonas aeruginosa bacteria." Tikrit Journal of Pure Science 25, no. 2 (2020): 86. http://dx.doi.org/10.25130/j.v25i2.962.

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The aim of this study was to assess effects of the 532nm Nd-yag laser on the genes of Tox A, Exo S, and Opr L, of Pseudomonas aeruginosa (P. aeruginosa) bacteria isolated from clinical (wounds, burns, otitis media) and environmental (water, soil) samples. Clinical samples were collected from patients coming to Saladdin General Hospital from wound, burns and middle ear infections while environmental samples were extracted from water and soil for Saladdin General Hospital . Bacterial samples irradiated by Nd-Yag laser with wavelength of 532 nm using energies (300mj,500mj) with (15 and 25 sec) an

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15

Кишилова, С. А., В. А. Леонова, И. В. Рожкова та О. Ю. Фоменко. "Сравнительная оценка ферментативной активности штаммов Pseudomonas aeruginosa, выделенных на молочных производствах". Food processing industry, № 4 (3 квітня 2025): 140–45. https://doi.org/10.52653/ppi.2025.4.4.026.

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В настоящее время микробиота, ответственная за порчу молока, представлена в основном психротрофными микроорганизмами, среди которых доминируют представители псевдомонад. Способность псевдомонад к росту в охлажденном молоке с одновременным продуцированием термостабильных ферментов порчи, не разрушающихся при технологических температурных воздействиях, является серьезной проблемой для молочной промышленности. Из представителей псевдомонад наиболее эпидемически значимой является условно-патогенная бактерия Pseudomonas aeruginosa, обладающая многофакторными механизмами адаптации к разнообразным ст

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Mustafa, Sameer Midhat, and Maher Abed Suha. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. https://doi.org/10.5281/zenodo.7924953.

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The bacteria <em>Pseudomonas aeruginosa</em> were isolated and diagnosed and compared with <em>Pseudomonas fluorescens</em> for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and <em>Pseudomonas fluorescens</em> and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitativ

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Mikucionyte, Greta, Asta Dambrauskiene, Erika Skrodeniene, and Astra Vitkauskiene. "Biofilm formation and serum susceptibility in Pseudomonas aeruginosa." Open Medicine 9, no. 2 (2014): 187–92. http://dx.doi.org/10.2478/s11536-013-0241-y.

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AbstractPseudomonas aeruginosa (P. aeruginosa) is one of the most important opportunistic pathogens. The pathogenicity of P. aeruginosa has been associated with multiple bacterial virulence factors. The aim of this study was to evaluate the association between P. aeruginosa strains obtained from various clinical samples and resistance to antibiotics and pathogenicity factors, such as resistance to serum bactericidal activity and biofilm formation. This study included 121 P. aeruginosa strains isolated from clinical samples; 65 of the isolated P. aeruginosa strains were carbapenem-resistant, an

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Young, Heather, Bryan Knepper, Whitney Hernandez, et al. "Pseudomonas aeruginosa." Journal of the American Podiatric Medical Association 105, no. 2 (2015): 125–29. http://dx.doi.org/10.7547/0003-0538-105.2.125.

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Background Pseudomonas aeruginosa has traditionally been considered a common pathogen in diabetic foot infection (DFI), yet the 2012 Infectious Diseases Society of America guideline for DFI states that “empiric therapy directed at P aeruginosa is usually unnecessary.” The objective of this study was to evaluate the frequency of P aeruginosa isolated from bone or tissue cultures from patients with DFI. Methods This study is a cross-sectional survey of diabetic patients presenting with a foot infection to an urban county hospital between July 1, 2012, and December 31, 2013. All of the patients h

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Salter, Susannah J. "Keeping an eye on P. aeruginosa." Nature Reviews Microbiology 13, no. 2 (2014): 69. http://dx.doi.org/10.1038/nrmicro3422.

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Wagner, Victoria E., and Barbara H. Iglewski. "P. aeruginosa Biofilms in CF Infection." Clinical Reviews in Allergy & Immunology 35, no. 3 (2008): 124–34. http://dx.doi.org/10.1007/s12016-008-8079-9.

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Nishiyama, Yuri, Asuka Mizutani, Masato Kobayashi, et al. "SPECT Imaging of P. aeruginosa Infection in Mice Using 123I-BMIPP." Pharmaceutics 16, no. 5 (2024): 656. http://dx.doi.org/10.3390/pharmaceutics16050656.

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Pseudomonas aeruginosa infection is an infectious disease that must be controlled because it becomes chronic and difficult to treat, owing to its unique system of toxin production/injection and elimination of other bacteria. Here, we noninvasively monitored P. aeruginosa using single-photon emission computed tomography (SPECT) imaging. Determining the amount and localization of the P. aeruginosa will enable making faster clinical diagnoses and selecting the most appropriate therapeutic agents and methods. Nonclinically, this information can be used for imaging in combination with biofilms and

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Paliy, G. K., О. А. Nazarchuk, V. І. Nagaychuk, І. М. Vovk та G. G. Nazarchuk. "ОБГРУНТУВАННЯ ДОЦІЛЬНОСТІ ЗАСТОСУВАННЯ ДЕКАМЕТОКСИНУ ПРИ АНТИБІОТИКО– ТА ФАГОРЕЗИСТЕНТНОСТІ ПСЕВДОМОНАДНОЇ ХІРУРГІЧНОЇ ІНФЕКЦІЇ". Klinicheskaia khirurgiia, № 9 (29 липня 2017): 64. http://dx.doi.org/10.26779/2522-1396.2017.09.64.

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Вступ. Pseudomonas aeruginosa (P. aeruginosa) є провідним збудником інфекційних ускладнень у пацієнтів комбустіологічних віддалень. Мета. Обгрунтувати доцільність використання декаметоксину (ДМ) при резистентності P. aeruginosa до цефалоспоринів. Матеріали і методи. Вивчено ефективність використання ДМ в подоланні резистентності P. aeruginosa до цефалоспоринів,псевдомонадного бактеріофагу на 50 клінічних штамах, виділених у постраждалих з опіками. Результати. Встановлено, що штами P. aeruginosa, що спричиняли інфекційні ускладнення у постраждалих з опіками, мали виражену рези

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Murad, Khairiyah, Sharaniza Ab-Rahim, and Hassanain Al-Talib. "Antimicrobial effect of Tetraspanin CD9 Peptides on Pseudomonas aeruginosa." Journal of Pure and Applied Microbiology 17, no. 3 (2023): 1764–75. http://dx.doi.org/10.22207/jpam.17.3.41.

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It is critical to find an alternative therapeutic approach to combat Pseudomonas aeruginosa (P. aeruginosa) that can simultaneously reduce the occurrence of bacterial resistance. The tetraspanin CD9, a highly expressed membrane protein in melanocytes was chosen for this study because it is highly expressed in keratinocytes and has been implicated in the pathogenesis of bacterial infections in a previous study. The antimicrobial activity of CD9 peptides against the standard strain P. aeruginosa (ATCC 27853) and a clinical multidrug-resistant P. aeruginosa (MDR- P. aeruginosa) was studied using

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Ishida, Lilian Konno, Katsuhisa Ikeda, Noriko Tanno, Tomonori Takasaka, Kiyo Nishioka, and Yasuo Tanno. "Erythromycin Inhibits Adhesion of Pseudomonas Aeruginosa and Branhamella Catarrhalis to Human Nasal Epithelial Cells." American Journal of Rhinology 9, no. 1 (1995): 53–56. http://dx.doi.org/10.2500/105065895781874097.

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Ciliated epithelial cells were obtained from nasal polyps. Bacterial adherence to these cells was compared for the ability to bind Hemophilus influenzae, Pseudomonas aeruginosa, and Branhamella catarrhalis in the presence of 10–5 M erythromycin, which was comparable with a physiologically attainable concentration in the nasal secretion and the maxillary sinus mucosa. Quantification of bacterial adherence showed the strongest ability of P. aeruginosa to the cells. Erythromycin has an inhibitory effect on adherence of P. aeruginose and B. catarrhalis to the nasal epithelial cell. Our findings su

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Ikarashi, Kei, Ryo Kutsuna, Junko Tomida, Yoshiaki Kawamura, and Yuji Morita. "Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa." Antibiotics 10, no. 6 (2021): 658. http://dx.doi.org/10.3390/antibiotics10060658.

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Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly recognized as a primary determinant of aminoglycoside resistance in P. aeruginosa. MexXY overexpression is often observed in drug-resistant P. aeruginosa clinical isolates. MexXY deficiency increased pyoverdine production in all four P. aeruginosa strains we tested. MexXY-overproducing multidrug-re

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Lautenbach, Ebbing, Mark G. Weiner, Irving Nachamkin, Warren B. Bilker, Angela Sheridan, and Neil O. Fishman. "Imipenem Resistance Among Pseudomonas aeruginosa Isolates Risk Factors for Infection and Impact of Resistance on Clinical and Economic Outcomes." Infection Control & Hospital Epidemiology 27, no. 9 (2006): 893–900. http://dx.doi.org/10.1086/507274.

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Objectives.To identify risk factors for infection with imipenem-resistant Pseudomonas aeruginosa and determine the impact of imipenem resistance on clinical and economic outcomes among patients infected with P. aeruginosa.Designs.An ecologic study, a case-control study, and a retrospective cohort study.Setting.A 625-bed tertiary care medical center.Patients.All patients who had an inpatient clinical culture positive for P. aeruginosa between January 1, 1999, and December 31, 2000.Results.From 1991 through 2000, the annual prevalence of imipenem resistance among P. aeruginosa isolates increased

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Hamza, Dalia, and Hala M. Zaher. "Carriage of Rifampicin- and Multidrug-Resistant Pseudomonas aeruginosa in Apparently Healthy Camels: A View Through a Zoonosis Lens." Microbiology Research 16, no. 6 (2025): 107. https://doi.org/10.3390/microbiolres16060107.

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Pseudomonas aeruginosa poses a significant global concern in human and veterinary medicine due to its resistance to multiple antimicrobials. Limited research has been carried out on rifampicin-resistant P. aeruginosa, particularly in food-producing animals such as camels. Therefore, the purpose of this study was to investigate the occurrence of rifampicin- and multidrug-resistant P. aeruginosa in apparently healthy camels. Nasal swabs and tissue samples were collected from one hundred apparently healthy slaughtered camels, and they were subjected to bacteriological isolation and identification

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Chaudhary, Jitendra Kumar. "A Clinical-Bacteriological Profile of Multidrug Resistant Pseudomonas aeruginosa at Tertiary Care Hospital Shahjahanpur, Uttar Pradesh." International Journal of Current Microbiology and Applied Sciences 10, no. 9 (2021): 301–13. http://dx.doi.org/10.20546/ijcmas.2021.1009.035.

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The Pseudomonas aeruginosa is predominant agent causing nosocomial infections. In recent time, it develops resistance continuously to the antibiotics becomes Multidrug-resistant P. aeruginosa. So, in cystic fibrosis patents it difficult to eradicate P. aeruginosa infections with antimicrobial treatment. Therefore, focus on alternative mechanisms for treating P. aeruginosa infections. On the basis of growth, morphological and biochemical characteristics, P. aeruginosa strains were isolated from the clinical samples in this work. After that the antibiotic sensitivity was performed and the Multid

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Vitkauskienė, Astra, Erika Skrodenienė, Daiva Jomantienė, Andrius Macas, and Raimundas Sakalauskas. "Changes in the Dependence of Pseudomonas aeruginosa O Serogroup Strains and Their Resistance to Antibiotics in a University Hospital During a 5-year Period." Medicina 47, no. 7 (2011): 361. http://dx.doi.org/10.3390/medicina47070051.

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The aim of our study was to determine the changes in antibiotic resistance and O serogroup dependence of P. aeruginosa strains isolated from lower respiratory tract specimens of patients in 2003 and 2008; the patients were treated in intensive care units of the biggest treatment facility in Lithuania (Hospital of Lithuanian University of Health Sciences, HLUHS, former Hospital of Kaunas University of Medicine) Material and Methods. The study included 90 P. aeruginosa strains serotyped in 2003 and 101 P. aeruginosa strains serotyped in 2008, which were randomly selected. The resistance of P. ae

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Zaidi, Tanweer S., Jeffrey Lyczak, Michael Preston, and Gerald B. Pier. "Cystic Fibrosis Transmembrane Conductance Regulator-Mediated Corneal Epithelial Cell Ingestion of Pseudomonas aeruginosaIs a Key Component in the Pathogenesis of Experimental Murine Keratitis." Infection and Immunity 67, no. 3 (1999): 1481–92. http://dx.doi.org/10.1128/iai.67.3.1481-1492.1999.

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ABSTRACT Previous findings indicate that the cystic fibrosis transmembrane conductance regulator (CFTR) is a ligand for Pseudomonas aeruginosa ingestion into respiratory epithelial cells. In experimental murine keratitis, P. aeruginosa enters corneal epithelial cells. We determined the importance of CFTR-mediated uptake of P. aeruginosa by corneal cells in experimental eye infections. Entry of noncytotoxic (exoU) P. aeruginosa into human and rabbit corneal cell cultures was inhibited with monoclonal antibodies and peptides specific to CFTR amino acids 108 to 117. Immunofluorescence microscopy

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Han, Lu, Qinmei Ma, Jialin Yu, et al. "Autophagy plays a protective role during Pseudomonas aeruginosa-induced apoptosis via ROS–MAPK pathway." Innate Immunity 26, no. 7 (2020): 580–91. http://dx.doi.org/10.1177/1753425920952156.

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Pseudomonas aeruginosa infection can induce alveolar macrophage apoptosis and autophagy, which play a vital role in eliminating pathogens. These two processes are usually not independent. Recently, autophagy has been found to interact with apoptosis during pathogen infections. Nevertheless, the role of autophagy in P. aeruginosa-infected cell apoptosis is unclear. In this study, we explored the impact of P. aeruginosa infection on autophagy and apoptosis in RAW264.7 cells. The autophagy activator rapamycin was used to stimulate autophagy and explore the role of autophagy on apoptosis in P. aer

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Tutun, Soner, and Özen Yurdakul. "Importance of Pseudomonas aeruginosa in Food Safety and Public Health." Turkish Journal of Agriculture - Food Science and Technology 11, no. 10 (2023): 2016–26. http://dx.doi.org/10.24925/turjaf.v11i10.2016-2026.6155.

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Pseudomonas aeruginosa (P. aeruginosa), the most pathogenic species among the pseudomonas species, is a bacterium that causes opportunistic infections resulting in significant damage to host tissues. P. aeruginosa, which is resistant to antibiotics, also causes fatal infection in human and animals. Infections caused by P. aeruginosa are difficult to treat due to its rapid proliferation in the environment and its ability to form biofilms that confer resistance to antibiotics. One of the main virulence factors of P. aeruginosa is its direct damage to host tissues, which disrupts the host’s defen

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McCracken, Melissa G., Heather J. Adam, Joseph M. Blondeau, et al. "Characterization of carbapenem-resistant and XDR Pseudomonas aeruginosa in Canada: results of the CANWARD 2007–16 study." Journal of Antimicrobial Chemotherapy 74, Supplement_4 (2019): iv32—iv38. http://dx.doi.org/10.1093/jac/dkz285.

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Abstract Objectives Carbapenem-resistant Pseudomonas aeruginosa are emerging worldwide with increasing reports of carbapenemase-producing isolates. Carbapenem-resistant isolates may also be XDR. This study characterized carbapenem-resistant and XDR P. aeruginosa isolated from patients receiving care at Canadian hospitals from 2007 to 2016. Methods Antimicrobial susceptibility testing was performed using CLSI broth microdilution methods. PCR was used to detect carbapenemases (GES, KPC, NDM, IMP, VIM, OXA-48) and other resistance markers; specific carbapenemase gene variants were identified by D

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Jones, Leandra B., Sanjay Kumar, Courtnee’ R. Bell, et al. "Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition." Pathogens 8, no. 4 (2019): 297. http://dx.doi.org/10.3390/pathogens8040297.

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The packaging of molecular constituents inside extracellular vesicles (EVs) allows them to participate in intercellular communication and the transfer of biological molecules, however the role of EVs during bacterial infection is poorly understood. The goal of this study was to examine the effects of Pseudomonas aeruginosa (P. aeruginosa) infection on the biogenesis and composition of EVs derived from the mouse microglia cell line, BV-2. BV-2 cells were cultured in exosome-free media and infected with 0, 1.3 × 104, or 2.6 × 104 colony forming units per milliliter P. aeruginosa for 72 h. The re

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Tran, Nguyen Bao Vy, Quang Minh Truong, Lam Que Anh Nguyen, et al. "Prevalence and Virulence of Commensal Pseudomonas Aeruginosa Isolates from Healthy Individuals in Southern Vietnam (2018–2020)." Biomedicines 11, no. 1 (2022): 54. http://dx.doi.org/10.3390/biomedicines11010054.

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Understanding the colonization of Pseudomonas aeruginosa (P. aeruginosa) in healthy humans is useful for future prevention and treatment of P. aeruginosa infection. This study aimed to investigate the prevalence and risk factors of of P. aeruginosa colonization in healthy humans. At the same time, the virulence of the isolated P. aeruginosa was also studied. In the study, 609 Vietnamese volunteers (310 females and 299 males, age range of 2 to 73 years), who had no acute infection or disease symptoms participated at the time of sample collection. Samples were taken from the throat, nostrils, an

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TUMBARELLO, M., E. REPETTO, E. M. TRECARICHI, et al. "Multidrug-resistant Pseudomonas aeruginosa bloodstream infections: risk factors and mortality." Epidemiology and Infection 139, no. 11 (2011): 1740–49. http://dx.doi.org/10.1017/s0950268810003055.

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SUMMARYWe retrospectively studied patients diagnosed with P. aeruginosa bloodstream infections (BSIs) in two Italian university hospitals. Risk factors for the isolation of multidrug-resistant (MDR) or non-MDR P. aeruginosa in blood cultures were identified by a case-case-control study, and a cohort study evaluated the clinical outcomes of such infections. We identified 106 patients with P. aeruginosa BSI over the 2-year study period; 40 cases with MDR P. aeruginosa and 66 cases with non-MDR P. aeruginosa were compared to 212 controls. Independent risk factors for the isolation of MDR P. aerug

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Harris, Anthony D., Eli Perencevich, Mary-Claire Roghmann, Glenn Morris, Keith S. Kaye, and Judith A. Johnson. "Risk Factors for Piperacillin-Tazobactam-Resistant Pseudomonas aeruginosa among Hospitalized Patients." Antimicrobial Agents and Chemotherapy 46, no. 3 (2002): 854–58. http://dx.doi.org/10.1128/aac.46.3.854-858.2002.

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ABSTRACT Antimicrobial resistance is an emerging problem with Pseudomonas aeruginosa. This study determined risk factors for the recovery of piperacillin-tazobactam-resistant P. aeruginosa from clinical cultures from hospitalized patients. A case-control study design was used to compare two groups of case patients with control patients. The first group of case patients was defined by nosocomial isolation of piperacillin-tazobactam-resistant P. aeruginosa, and the second group of cases yielded piperacillin-tazobactam-susceptible P. aeruginosa. Controls were selected in a 6:1 ratio from the same

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Jameel, Zainab Hafidh, Mourouge Saadi Alwash, and Anwar A. Abdulla. "Molecular Detection, Antibiotic Resistance, and Biofilm Formation of Clinical Pseudomonas aeruginosa Isolates from Patients in Al-Hillah, Babylon Province, Iraq." Medical Journal of Babylon 21, no. 2 (2024): 292–97. http://dx.doi.org/10.4103/mjbl.mjbl_388_23.

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Abstract Background: Pseudomonas aeruginosa is known as one of the main causes of nosocomial infections and is repeatedly related to opportunistic infections among hospitalized patients worldwide. Antibiotic resistance (AR) and biofilm-forming ability (BFA) are two major virulence factors of P. aeruginosa isolates that are involved in the continuation of infections. Objectives: This study aimed to isolate and identify P. aeruginosa isolates from patients and to elucidate the correlation between AR and BFA among isolates. Materials and Methods: A total of 130 clinical P. aeruginosa isolates wer

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Monteiro, Rosana, Andreia Patrícia Magalhães, Maria Olivia Pereira, and Ana Margarida Sousa. "Long-term coexistence of Pseudomonas aeruginosa and Staphylococcus aureus using an in vitro cystic fibrosis model." Future Microbiology 16, no. 12 (2021): 879–93. http://dx.doi.org/10.2217/fmb-2021-0025.

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Aim: To investigate the role of pre-established Staphylococcus aureus on Pseudomonas aeruginosa adaptation and antibiotic tolerance. Materials & methods: Bacteria were cultured mimicking the sequential pattern of lung colonization and exposure to ciprofloxacin. Results: In the absence of ciprofloxacin exposure, S. aureus and P. aeruginosa coexisted supported by the physicochemical characteristics of the artificial sputum medium. S. aureus had no role in P. aeruginosa tolerance against ciprofloxacin and did not select P. aeruginosa small-colony variants during antibiotic treatment. rhlR and

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ERAKY, R. D., W. A. ABD EL-GHANY, and K. M. SOLIMAN. "Studies on Pseudomonas aeruginosa Infection in Hatcheries and Chicken." Journal of the Hellenic Veterinary Medical Society 71, no. 1 (2020): 1953. http://dx.doi.org/10.12681/jhvms.22937.

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The aim of this work was to spot light on the presence of Pseudomonas aeruginosa (P. aeruginosa) strains in hatcheries and dead in shell embryos. A total of 406 samples representing 200 and 206 swabs from hatcheries environment and yolk sacs of dead in shell embryos were collected from Damietta governorate, Egypt. P. aeruginosa was isolated and identified. Some virulent genes (toxA, psIA and fliC) of P. aeruginosa were detected using polymerase chain reaction (PCR). The antimicrobial susceptibility of P. aeruginosa was tested in vitro. Day and 11 days old broiler chicks were challenged with P.

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Nguyen, Thi Phuong Truc, Nhu Ngoc Nguyen, Dinh Ky Lim, Thuc Quyen Huynh, and Thi Thu Hoai Nguyen. "Development of a loop-mediated amplification (Lamp) assay for detection of environmental <i>Pseudomonas aeruginosa</i>." Vietnam Journal of Biotechnology 22, no. 4 (2024): 616–29. https://doi.org/10.15625/vjbt-21649.

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Pseudomonas aeruginosa (P. aeruginosa) is a common environmental bacterium found in various habitats, including water and soil. Rapid detection of this microorganism is essential for monitoring environmental contamination and assessing its potential impact on ecosystems and public health. This study aimed to develop a high-efficiency loop-mediated isothermal amplification (LAMP) assay targeting a P. aeruginosa-specific gene encoding a hypothetical protein (GenBank ID: 882161). The study involved two main parts: 1) Isolation and identification of P. aeruginosa from environmental samples, and 2)

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Carles, Michel, Mathieu Lafargue, Arnaud Goolaerts, et al. "Critical Role of the Small GTPase RhoA in the Development of Pulmonary Edema Induced by Pseudomonas aeruginosa in Mice." Anesthesiology 113, no. 5 (2010): 1134–43. http://dx.doi.org/10.1097/aln.0b013e3181f4171b.

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Background Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe pneumonia in critically ill patients. We have reported previously that P. aeruginosa exotoxins S and T mediate in vitro the increase in protein permeability across lung endothelial cell monolayers via a RhoA-dependent mechanism. However, whether inhibition of RhoA would significantly attenuate P. aeruginosa-mediated lung injury in mice is unknown. Methods P. aeruginosa-induced paracellular protein permeability was measured across bovine lung endothelial and rat alveolar epithelial type II cell monolayers with

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Thi, Minh Tam Tran, David Wibowo, and Bernd H. A. Rehm. "Pseudomonas aeruginosa Biofilms." International Journal of Molecular Sciences 21, no. 22 (2020): 8671. http://dx.doi.org/10.3390/ijms21228671.

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Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Its highly notorious persistence in clinical settings is attributed to its ability to form antibiotic-resistant biofilms. Biofilm is an architecture built mostly by autogenic extracellular polymeric substances which function as a scaffold to encase the bacteria together on surfaces, and to protect them from environmental stresses, impedes phagocytosis and thereby conferring the capacity for colonization and long-term persistence. Here we rev

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Song, Jae-Jun, Byung Don Lee, Koen Hyeong Lee, Jong Dae Lee, Young Joo Park, and Moo Kyun Park. "Changes in Antibiotic Resistance in Recurrent Pseudomonas Aeruginosa Infections of Chronic Suppurative Otitis Media." Ear, Nose & Throat Journal 95, no. 10-11 (2016): 446–51. http://dx.doi.org/10.1177/0145561316095010-1107.

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This study investigated the changes in antibiotic resistance in recurrent Pseudomonas aeruginosa infections in chronic suppurative otitis media (CSOM). Its aim was to provide a treatment strategy for P aeruginosa infections in CSOM for the prevention of multidrug resistance. A case-control study was conducted in tertiary teaching hospitals in Korea. The experimental group included patients with recurrent P aeruginosa infection who had relapsed within 2 months after the successful control of a previous P aeruginosa infection. The control group consisted of patients with a P aeruginosa infection

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Cavalcante, Luzia Angélica Alves, Jéssica Martins de Andrade, Maria Goretti Varejão da Silva, et al. "Pseudomonas aeruginosa biofilm formers in drinking water." Núcleo do Conhecimento 01, no. 09 (2023): 17–29. https://doi.org/10.32749/nucleodoconhecimento.com.br/veterinaria-en/pseudomonas-aeruginosa.

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Currently there has been an increase in demand for bottled water, this is due to the population's dissatisfaction with the quality of water provided by public agencies. The present study sought to evaluate the presence of biofilm-forming P. aeruginosa in bottled drinking water. Thirty-five typical bottled water samples were selected to identify the presence of P. aeruginosa and its ability to form bacterial biofilm. The methodology used to verify the presence of P. aeruginosa followed the multiple tube technique and the evaluation of biofilm forming capacity followed the microdilution techniqu

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Hernández-Moreno, Lida V., Ludy C. Pabón-Baquero, Juliet A. Prieto-Rodriguez, and Oscar J. Patiño-Ladino. "Bioactive Compounds from P. pertomentellum That Regulate QS, Biofilm Formation and Virulence Factor Production of P. aeruginosa." Molecules 28, no. 17 (2023): 6181. http://dx.doi.org/10.3390/molecules28176181.

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Pseudomonas aeruginosa is an opportunistic pathogen responsible for many nosocomial infections. This bacterium uses Quorum Sensing (QS) to generate antimicrobial resistance (AMR) so its disruption is considered a novel approach. The current study describes the antibiofilm and QS inhibitory potential of extract and chemical components from Piper pertomentellum. The methodo- logy included the phytochemical study on the aerial part of the species, the determination of QS inhibition efficacy on Chromobacterium violaceum and the evaluation of the effect on biofilm formation and virulence factors on

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Lee, Alfred, Dar Chow, Brian Haus, et al. "Airway epithelial tight junctions and binding and cytotoxicity ofPseudomonas aeruginosa." American Journal of Physiology-Lung Cellular and Molecular Physiology 277, no. 1 (1999): L204—L217. http://dx.doi.org/10.1152/ajplung.1999.277.1.l204.

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The role of tight junctions in the binding and cytoxicity of Pseudomonas aeruginosato apical or basolateral membranes of lung airway epithelial cells was tested with fluorescence microscopy on living cells. Binding of noncytotoxic P. aeruginosa strain O1 was assessed with P. aeruginosa that expressed green fluorescent protein. Binding of cytotoxic P. aeruginosa strain 6206 was assessed with FITC-labeled P. aeruginosa; cytotoxicity was determined from nuclear uptake of the impermeant dye propidium iodide. The role of direct contact of P. aeruginosa to epithelial cells was tested with filters wi

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Mahboub, Radia, and Faiza Memmou. "Antimicrobial Properties of 6-Bromoeugenol and Eugenol." International Letters of Natural Sciences 53 (April 1, 2016): 57–64. http://dx.doi.org/10.56431/p-4u2geq.

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We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains: Pseudomonas aeruginosa (S1), Escherichia coli (S2) and Staphylococcus aureus (S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with the Pseudomonas aeruginosa (S1) at all concentrations and w

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Duplantier, Marine, Elodie Lohou, and Pascal Sonnet. "Quorum Sensing Inhibitors to Quench P. aeruginosa Pathogenicity." Pharmaceuticals 14, no. 12 (2021): 1262. http://dx.doi.org/10.3390/ph14121262.

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The emergence and the dissemination of multidrug-resistant bacteria constitute a major public health issue. Among incriminated Gram-negative bacteria, Pseudomonas aeruginosa has been designated by the WHO as a critical priority threat. During the infection process, this pathogen secretes various virulence factors in order to adhere and colonize host tissues. Furthermore, P. aeruginosa has the capacity to establish biofilms that reinforce its virulence and intrinsic drug resistance. The regulation of biofilm and virulence factor production of this micro-organism is controlled by a specific bact

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Mueller-Ortiz, Stacey L., Travis J. Hollmann, David L. Haviland, and Rick A. Wetsel. "Ablation of the complement C3a anaphylatoxin receptor causes enhanced killing ofPseudomonas aeruginosain a mouse model of pneumonia." American Journal of Physiology-Lung Cellular and Molecular Physiology 291, no. 2 (2006): L157—L165. http://dx.doi.org/10.1152/ajplung.00358.2005.

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The C3a anaphylatoxin is a 77-amino acid peptide that is generated by enzymatic cleavage of C3 during activation of the complement system. C3a mediates numerous biological functions on binding its receptor (C3aR), which is present on both myeloid and nonmyeloid cells. To investigate the biological impact of C3a-mediated effects during acute pneumonia caused by Pseudomonas aeruginosa, we subjected C3aR-deficient mice and matched wild-type (WT) mice to P. aeruginosa pulmonary infection. C3aR-deficient mice exhibited increased killing of P. aeruginosa in the lungs, less dissemination of bacteria

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