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Journal articles on the topic 'P fluorescens rdna sequencing'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Fu, Yan-Mei, Hong-Bin Liu, and Xiao-Qin Wu. "Diversity and Function of Endo-Bacteria in Bursaphelenchus xylophilus from Pinus massoniana Lamb. in Different Regions." Forests 11, no. 5 (2020): 487. http://dx.doi.org/10.3390/f11050487.

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The pine wood nematode (PWN) Bursaphelenchus xylophilus is the pathogen that causes pine wilt disease (PWD), a devastating forest disease. PWN-associated bacteria may play a role in PWD. However, little is known about the endo-bacteria in PWN. We analyzed the diversity of endo-bacteria in nine isolates of PWNs from Pinus massoniana Lamb. in nine epidemic areas from three Chinese provinces by high-throughput sequencing of 16S rDNA and isolated and identified culturable endo-bacteria through construction of a 16S rDNA phylogenetic tree and Biolog microbial identification. We also examined the ef

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Said, Layla Abdulhamed, Sawsan Saeed Hasan, and Saad L. Hamed. "Identification of Clinical Pseudomonas spp. by VITEK 2 Compact System and Species-specific Polymerase Chain Reaction Assay for Identification of Pseudomonas aeruginosa." International Journal of Drug Delivery Technology 10, no. 03 (2020): 383–88. http://dx.doi.org/10.25258/ijddt.10.3.14.

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The objective of this study was to isolate, identify, and diagnose Pseudomonas spp. from different clinical sources in Baghdad, Iraq. VITEK 2 compact system identification gram-negative bacteria (ID gNB) cards were used to confirm the identification. Polymerase chain reaction (PCR) technique and sequencing were used for recognition of the 16S rDNA gene, by two pairs of primers, universal primers (930 bp fragments) for recognition of Pseudomonas spp., and Pseudomonas aeruginosa specific species (PASS) primers (956 bp fragments) for differentiation of P. aeruginosa from other species. Amplified

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Joshi, Bhavesh M., Mrugesh M. Patel, Rudra B. Parmar, N. K. Singh, and P. V. Tapre. "Molecular Characterization of Plant Growth Promoting Pseudomonas from Rhizosphere of Euphorbia caducifolia Haines." International Journal of Bio-resource and Stress Management 13, no. 11 (2022): 1240–51. http://dx.doi.org/10.23910/1.2022.3262.

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A study was conducted during kharif 2020 at the Department of Microbiology, Chimanbhai Patel College of Agriculture, S. D. Agricultural University for molecular characterization of plant growth promoting Pseudomonas isolates from rhizosphere of Euphorbia caducifolia. Twenty Pseudomonas isolates (designated as PsEc1 to PsEc20) were axenically isolated from rhizospheric soil sample of Euphorbia caducifolia using Pseudomonas Isolation Agar Base (a selective medium). These isolates were tested gram negative, rod shaped, and oxidase positive. All 20 isolates were positive for utilization of maltose

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Hassanshahian, Mehdi. "Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils." Iranian Journal of Toxicology 10, no. 3 (2016): 13–20. http://dx.doi.org/10.32598/ijt.10.4.357.1.

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Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA. Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environm

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Milijasevic-Marcic, Svetlana, Biljana Todorovic, Milos Stepanovic, et al. "Monitoring of bacterial diseases of Agaricus bisporus in Serbia." Pesticidi i fitomedicina 31, no. 1-2 (2016): 29–35. http://dx.doi.org/10.2298/pif1602029m.

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Monitoring of button mushroom bacterial diseases was conducted to estimate the presence and identity of mycopathogenic bacteria and to determine the predominant bacterial pathogen in Serbia. Samples were collected from mushroom farms during 2006- 2010 and also from fresh markets during 2014-2015. The collected samples showed either symptoms of brown blotch or different degrees of brown discoloration on caps and stalks of Agaricus bisporus resembling bacterial infection. The presence of bacterial droplets on gills was not recorded. The isolated bacteria were Gram-negative and fluorescent on Kin

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Marques, Eder, Rafaela CF Borges, and Carlos H. Uesugi. "Identification and pathogenicity of Pseudomonas cichorii associated with a bacterial blight of gerbera in the Federal District." Horticultura Brasileira 34, no. 2 (2016): 244–48. http://dx.doi.org/10.1590/s0102-053620160000200015.

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ABSTRACT: In 2013, leaf samples of gerbera plants showing symptoms of bacterial blight were collected in cut-flower high tunnels, in the settlement of núcleo rural alexandre gusmão, located in brazlândia, distrito federal, brazil. seven isolates obtained were subjected to phenotypic and molecular characterization, including pathogenicity tests, lopat, and partial sequencing of the 16s rdna gene. all isolates were gram-negative, aerobic, oxidase-positive, produced fluorescent pigment, induced hypersensitivity in tobacco leaves, used sorbitol and glutamate and were pathogenic to 24 different pla

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Li, Binbin, Shuji Liu, Xiaoting Chen, et al. "Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage." Foods 12, no. 21 (2023): 3994. http://dx.doi.org/10.3390/foods12213994.

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The quality changes, dynamic changes in microbial composition, and diversity changes in large yellow croaker (Larimichthys crocea) during 4 °C refrigeration were studied using 16S rDNA high-throughput sequencing technology, and the total viable count (TVC), total volatile basic nitrogen (TVB-N), and thiobarbituric acid-reactive substances (TBARS) were determined. The results revealed a consistent increase in TVC, TVB-N, and TBARS levels over time. On the 9th day, TVC reached 7.43 lg/(CFU/g), while on the 15th day, TVB-N exceeded the upper limit for acceptable quality, reaching 42.56 mg/100 g.

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Sharma, Garima, Shweta Dang, Sanjay Gupta, and Reema Gabrani. "IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BACTERIA HAVING ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 10 (2016): 111. http://dx.doi.org/10.22159/ijpps.2016v8i10.12338.

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<p><strong>Objective:</strong> The aim of the current study was to isolate and identify the bacteriocinogenic strain exhibiting broad range antimicrobial activity and antibiofilm activity from soil of animal farms.</p><p><strong>Methods:</strong> In the current study, bacterial strains were isolated from soil of twelve different regions of animal farm all over India and screened for antimicrobial activity against<em> Staphylococcus epidermidis</em>, <em>Micrococcus luteus, Pseudomonas fluorescence</em> and <em>Escherichia

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9

Koike, Steven T., Jeri D. Barak, Diana M. Henderson, and Robert L. Gilbertson. "Bacterial Blight of Leek: A New Disease in California Caused by Pseudomonas syringae." Plant Disease 83, no. 2 (1999): 165–70. http://dx.doi.org/10.1094/pdis.1999.83.2.165.

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During 1996 and 1997, a new and damaging disease of leek (Allium porrum) was observed on greenhouse-produced transplants and field-grown plants in California. Symptoms were water-soaked lesions at leaf tips, which eventually expanded down the length of the leaf and resulted in brown, elongated, stripe-like lesions with yellow margins. Diseased leaves eventually wilted. A blue fluorescent pseudomonad was consistently recovered from lesions, and biochemical and physiological tests indicated that it was Pseudomonas syringae. Pathogenicity tests established that representative strains of this P. s

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Akhtar, Khalid Pervaiz, Muhammad Yussouf Saleem, Sumaira Yousaf, Najeeb Ullah, Ghulam Rasool, and Nighat Sarwar. "Occurrence, identification and transmission of the phytoplasma associated with tomato big bud disease and identification of its vector and weed host in Pakistan." Archives of Phytopathology and Plant Protection 51, no. 7 (2018): 387–98. http://dx.doi.org/10.1080/03235408.2018.1485827.

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Tomato (Solanum lycopersicum L.) plants showing stunting, big bud, leaves yellowing or reddening and witches’-broom symptoms were observed since 2009 in Pakistan. A weed Parthenium hysterophorus grown in and around tomato fields also exhibited witches’-broom like symptoms. Fluorescence light microscopy of hand-cut stem stalk sections treated with Dienes’ stain showed blue areas in the phloem region of both tomato and P. hysterophorus symptomatic plants that indicated the association of phytoplasma with the complex. Amplification of 1.2 kb 16S rDNA fragment in nested PCR confirmed that the symp

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Faltin, Franziska, Jana Lottmann, Rita Grosch, and Gabriele Berg. "Strategy to select and assess antagonistic bacteria for biological control ofRhizoctonia solaniKühn." Canadian Journal of Microbiology 50, no. 10 (2004): 811–20. http://dx.doi.org/10.1139/w04-063.

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A screening strategy was developed to assess the potential of plant-associated bacteria to control diseases caused by Rhizoctonia solani Kühn. About 434 already characterized antagonistic bacterial strains isolated from diverse plant species and microenvironments were evaluated for biocontrol and plant growth promotion by a hierarchical combination of assays. Analyzing in vitro antagonism towards different Rhizoctonia isolates resulted in a selection of 20 potential biocontrol agents. The strains were characterized by their antagonistic mechanisms in vitro as well as their production of the pl

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Terlizzi, F., A. R. Babini, C. Lanzoni та ін. "First Report of a γ 3-Proteobacterium Associated with Diseased Strawberries in Italy". Plant Disease 91, № 12 (2007): 1688. http://dx.doi.org/10.1094/pdis-91-12-1688b.

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During the fall seasons of 2005 and 2006, diseased strawberry plants (Fragaria × ananassa Duch.) were observed in nurseries and production fields in Ferrara, Forli-Cesena, and Ravenna provinces (Emilia-Romagna region, northern Italy). Symptoms consisted of a conspicuous plant stunting with a poor root system. Older leaves rolled upward and displayed a marked premature purplish discoloration, while young leaves were cupped, chlorotic, generally reduced in size, and had shortened petioles. This strawberry disorder was similar to “marginal chlorosis”, an infectious disease occurring in France tha

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Gumtow, R. L., A. A. Khan, A. M. Bocsanczy, J. M. F. Yuen, A. J. Palmateer, and D. J. Norman. "First Report of a Leaf Spot Disease of Golden Dewdrop (Duranta erecta) Caused by Pseudomonas cichorii and a Xanthomonas Species in Florida." Plant Disease 97, no. 6 (2013): 836. http://dx.doi.org/10.1094/pdis-12-12-1117-pdn.

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Duranta erecta (Verbenaceae) is used extensively in southern states as an ornamental shrub and has replaced boxwood as the most common short hedge accenting flower beds. Over the past 2 years, during warm wet periods, dark necrotic leaf spots have been observed on golden dewdrop plants in Florida. Isolations from these spots on Difco nutrient agar (NA) consistently yielded two types of bacterial colonies that were not always simultaneously present: 1) round butyrous, bright yellow and 2) flat cream-colored. Both were 2 mm in size after 48 h, gram-negative, and produced a hypersensitivity react

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P. Bhosle, Nilesh. "Biodegradation of Carbendazim (CRD) by Microorganisms Isolated from Pesticides Contaminated Soil." Journal of Plant Science Research 38, no. 2 (2023): 861–74. http://dx.doi.org/10.32381/jpsr.2022.38.02.40.

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Excessive use of pesticides has been known to create hazardous to the environment, including affecting soil fertility may also impart toxicity in living organisms. Presently, there have been biological approaches implicated to reduce or eradicate pesticides through physical and chemical methods are inefficient. Microorganisms have a potential to mineralize carbendazim with the help of nutrient minimal mineral salt (MMS) medium and soil. Microorganisms were developed resistant against carbendazim MMS medium and they were identified based on its colony morphological characters and 16s rDNA gene

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15

Mølbak, Lars, Tine Rask Licht, Thomas Kvist, Niels Kroer, and Sigrid Rita Andersen. "Plasmid Transfer from Pseudomonas putida to the Indigenous Bacteria on Alfalfa Sprouts: Characterization, Direct Quantification, and In Situ Location of Transconjugant Cells." Applied and Environmental Microbiology 69, no. 9 (2003): 5536–42. http://dx.doi.org/10.1128/aem.69.9.5536-5542.2003.

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ABSTRACT The transfer of the plasmids pJKJ5 and TOL (pWWO) from Pseudomonas putida to the indigenous bacterial community on alfalfa sprouts was studied. Tagging with fluorescent protein markers allowed direct quantification of the introduced donor bacteria and of indigenous bacteria that had received the plasmids. The sprouts were observed for 9 days; during this time alfalfa seeds, inoculated with donor bacteria, developed to edible and subsequently decaying sprouts. The first transconjugants were detected on day 6 after donor inoculation and occurred at frequencies of 3.4 × 10−4 and 2.0 × 10

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Fu, Pei P., Fan Xiong, Shan G. Wu, et al. "Effects of Schyzocotyle acheilognathi (Yamaguti, 1934) infection on the intestinal microbiota, growth and immune reactions of grass carp (Ctenopharyngodon idella)." PLOS ONE 17, no. 4 (2022): e0266766. http://dx.doi.org/10.1371/journal.pone.0266766.

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Our understanding of interactions among intestinal helminths, gut microbiota and host is still in its infancy in fish. In this study, the effects of Schyzocotyle acheilognathi infection on the intestinal microbiota, growth and immune reactions of grass carp were explored under laboratory conditions. 16S rDNA amplification sequencing results showed that S. acheilognathi infection altered the composition of intestinal microbiota only at the genus level, with a significant increase in the relative abundance of Turicibacter and Ruminococcus (P < 0.05) and a significant decrease in the relative

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17

Zhou, L. H., Y. Han, G. H. Ji, Z. S. Wang, and F. Liu. "First Report of Bacterial Leaf Spot Disease Caused by Pseudomonas syringae pv. syringae on Panax notoginseng." Plant Disease 97, no. 5 (2013): 685. http://dx.doi.org/10.1094/pdis-11-12-1047-pdn.

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Panax notoginseng is a species that produces a rare type of Chinese herbal medicine and is cultivated primarily in Yunnan Province. P. notoginseng has a 3-year-long crop cycle before harvest. A new bacterial disease was observed on P. notoginseng plants in the Wenshan Mountain area of Yunnan in 2012. The disease affected primarily leaves. Symptoms were circular or irregular brown leaf spots, surrounded by a yellow halo, located on the edges of the leaves. Eight creamy white pigmented, rounded strains were isolated consistently from leaf spots on Luria-Bertani agar (LB) medium, incubated at 28°

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18

Reider, S., W. Posch, R. Koch, et al. "P917 Intestinal complement interacts with commensal microbiota and provides pattern recognition in Inflammatory Bowel Disease." Journal of Crohn's and Colitis 17, Supplement_1 (2023): i1027. http://dx.doi.org/10.1093/ecco-jcc/jjac190.1047.

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Abstract Background The complement system is a central humoral part of innate immunity and provides essential pattern recognition capabilities as well as effector functions such as opsonisation and target cell lysis. Its prominent role in infection and immunity is undisputed but its involvement in the pathogenesis of inflammatory bowel diseases remains less clear. Published data point to a role of complement within the intestinal lumen supporting clearance of pathogens1. Methods Expression of complement proteins within the intestinal epithelium was quantified by real-time qPCR on lesional and

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Zepp Falz, K., C. Holliger, R. Großkopf, et al. "Vertical Distribution of Methanogens in the Anoxic Sediment of Rotsee (Switzerland)." Applied and Environmental Microbiology 65, no. 6 (1999): 2402–8. http://dx.doi.org/10.1128/aem.65.6.2402-2408.1999.

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ABSTRACT Anoxic sediments from Rotsee (Switzerland) were analyzed for the presence and diversity of methanogens by using molecular tools and for methanogenic activity by using radiotracer techniques, in addition to the measurement of chemical profiles. After PCR-assisted sequence retrieval of the 16S rRNA genes (16S rDNA) from the anoxic sediment of Rotsee, cloning, and sequencing, a phylogenetic analysis identified two clusters of sequences and four separated clones. The sequences in cluster 1 grouped with those of Methanosaeta spp., whereas the sequences in cluster 2 comprised the methanogen

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Balaž, J., R. Iličić, S. Maširević, D. Jošić, and S. Kojić. "First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia." Plant Disease 98, no. 5 (2014): 684. http://dx.doi.org/10.1094/pdis-07-13-0714-pdn.

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Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only in the north (Vojvodina Province), up to 1,500 ha. In June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent and greasy spots with yellow margins) were observed for the first time, cultivated at the experimental fields near Backi Petrovac. Since then, bacterial spots were regularly observed on oil pumpkin in the beginning of the growing seasons and during rainy weather, with disease incidence ranging from 5 to 20%. Bacteria isolated

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Balestra, G. M., A. Mazzaglia, and A. Rossetti. "Outbreak of Bacterial Blossom Blight Caused by Pseudomonas viridiflava on Actinidia chinensis Kiwifruit Plants in Italy." Plant Disease 92, no. 12 (2008): 1707. http://dx.doi.org/10.1094/pdis-92-12-1707a.

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During the springs from 2006 to 2008, a new disease was observed on 4- to 5-year-old Actinidia chinensis (cv. Jin Tao) trees in different commercial kiwifruit-production areas in northern Italy (Lombardy). Initially, disease occurrence was sporadic but later became widespread. Symptoms on flowers appeared as a dark brown rot of anthers, filaments, sepals, and whole buds. Blossoms abscised prematurely from buds. Symptoms appeared as confluent brown spots often present on rolled margins. Bacteria were isolated from symptomatic tissue on nutrient agar medium supplemented with 5% sucrose. The isol

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Teshome, Befekadu, Eleni Belay, Barkelegne Mengesha, Zerihun Tsegaye, Edwin K. Akley, and Awuku Frederick. "Selection and identification of Pseudomonas and Bacillus Rhizobacteria with Bioinoculant potential for Sorghum." International Journal of Science, Technology, Engineering & Mathematics 5, no. 2 (2025): 38–59. https://doi.org/10.53378/ijstem.353191.

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This study aimed to select and identify elite strains of plant growth-promoting rhizobacteria (PGPR) among phosphate-solubilizing bacteria (PSB) conserved at the Ethiopian Biodiversity Institute. In-vitro screening for plant growth-promoting traits and abiotic stress tolerance identified 12 promising PGPR isolates. These strains exhibited phosphate solubilization (index 1.8–3.2), auxin production, nitrogen fixation, and ammonia production. All isolates showed drought tolerance, and some exhibited salt tolerance (up to 10% NaCl). 16S rDNA sequencing identified Pseudomonas frederiksbergensis, Ps

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Lou, Haibo, Xiaobing Wang, Jun Chen, Bozhi Wang, and Wei Wang. "Transcriptomic response ofRalstonia solanacearumto antimicrobialPseudomonas fluorescensSN15-2 metabolites." Canadian Journal of Microbiology 64, no. 11 (2018): 816–25. http://dx.doi.org/10.1139/cjm-2018-0094.

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To develop efficient biocontrol agents, it is essential to investigate the response of soil-borne plant pathogens to such agents. For example, the response of Ralstonia solanacearum, the tomato wilt pathogen, to antimicrobial metabolites of Pseudomonas fluorescens is unknown. Thus, we assessed the effects of P. fluorescens SN15-2 fermentation broth on R. solanacearum by transmission electron microscopy and transcriptome technology. RNA sequencing identified 109 and 155 genes that are significantly upregulated and downregulated, respectively, in response to P. fluorescens metabolites, many of w

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Hurtado-Martinez, Miguel, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maximino Manzanera, and Jesus Gonzalez-Lopez. "Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors." Water 13, no. 8 (2021): 1119. http://dx.doi.org/10.3390/w13081119.

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Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denitrificans Z1, and P. fluorescens PSC26, previously reported as denitrifying microorganisms carrying the nosZ gene. Pseudomonas denitrificans Z1 produced fluffy, low-density granules, with a decantation speed below 10 m h−1. However, P. fluorescens PSC

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Abo-Elyousr, Kamal A. M., Maged M. Saad, Adel D. Al-Qurashi, Omer H. M. Ibrahim, and Magdi A. A. Mousa. "Management of Cumin Wilt Caused by Fusarium oxysporum Using Native Endophytic Bacteria." Agronomy 12, no. 10 (2022): 2510. http://dx.doi.org/10.3390/agronomy12102510.

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Eleven endophytic bacteria were isolated from the roots of cumin, a medicinal plant grown in Assiut Province, Egypt. The strains KAU2025 and KAU2022 exhibited the highest inhibition rate against Fusarium oxysporum (FOC7) (64%) in dual culture test and were selected for further studies. KAU2022 and KAU2025 were identified as Brevibacillus parabrevis and Pseudomonas fluorescens, respectively, based on 16S rDNA sequence analysis. The isolates increased the seed germination of cumin by 46.6% compared to that in the control infected with the pathogen F. oxysporum (FOC7). Additionally, the biocontro

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Karnwal, Arun, and Aradhana Dohroo. "Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan." F1000Research 7 (January 25, 2018): 112. http://dx.doi.org/10.12688/f1000research.13644.1.

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Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1)

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Jami Al-Ahmadi, Majid, Abbas Mohammadi, and Esmaeil Salehi Kohabadi. "Characterization of Bacteria Isolated from the Saffron (Crocus sativus L.) Rhizosphere." Journal of Horticultural Research 25, no. 2 (2017): 5–14. http://dx.doi.org/10.1515/johr-2017-0017.

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Abstract One purpose of assessing the soil alive and active community is the identification of beneficial bacteria to use them as biological fertilizers, replacing or supplementing synthetic fertilizers. Such biofertilizers are predicted for the sustainability of agricultural production, especially for low input systems such as saffron fields. The aim of this work was to isolate and identify saffron rhizobacteria and to evaluate their possible effects on saffron growth. During 2013/14, some bacteria were isolated from the rhizosphere of the saffron plantations of different age in Gol village,

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Lawton, Samantha J., Allison M. Weis, Barbara A. Byrne, et al. "Comparative analysis of Campylobacter isolates from wild birds and chickens using MALDI-TOF MS, biochemical testing, and DNA sequencing." Journal of Veterinary Diagnostic Investigation 30, no. 3 (2018): 354–61. http://dx.doi.org/10.1177/1040638718762562.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1). The test results for all 27 isolates were in 100% agreement between MALDI

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Benlioglu, K., Ü. Özyilmaz, and D. Ertan. "First Report of Bacterial Blight Caused by Pseudomonas syringae pv. pisi on Pea in Turkey." Plant Disease 94, no. 7 (2010): 923. http://dx.doi.org/10.1094/pdis-94-7-0923a.

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In April of 2009, leaf blight symptoms were observed on field peas (Pisum sativum L.) grown in Söke, Torbali, and Ödemis counties in the Aegean Region of Turkey. Field inspections revealed disease incidence as high as 45% and the disease was found in 13 commercial fields. Initial symptoms consisted of small, dark green, water-soaked lesions on leaves, stipules, and stems near ground level. Lesions often enlarged and coalesced and turned chocolate brown with a water-soaked margin. Stem infections usually coalesced and girdled the stem spreading upward to stipules and leaflets forming a fan-like

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Morimoto, Yuh, Mari Tohya, Zulipiya Aibibula, Tadashi Baba, Hiroyuki Daida, and Teruo Kirikae. "Re-identification of strains deposited as Pseudomonas aeruginosa, Pseudomonas fluorescens and Pseudomonas putida in GenBank based on whole genome sequences." International Journal of Systematic and Evolutionary Microbiology 70, no. 11 (2020): 5958–63. http://dx.doi.org/10.1099/ijsem.0.004468.

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The taxonomic classification of Pseudomonas species has been revised and updated several times. This study utilized average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) cutoff values of 95 and 70 %, respectively, to re-identify the species of strains deposited in GenBank as P. aeruginosa , P. fluorescens and P. putida . Of the 264 deposited P. aeruginosa strains, 259 were correctly identified as P. aeruginosa , but the remaining five were not. All 28 deposited P. fluorescens strains had been incorrectly identified as P. fluorescens . Four of these strains were re-identifi

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Lee, Spencer, and Robert Jonas. "Genomic Evolution in Pseudomonas fluorescens as a Result of Gradual Temperature Changes." Fine Focus 9, no. 1 (2023): 84–96. http://dx.doi.org/10.33043/ff.9.1.84-96.

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As climate change continues to affect global temperatures, organisms will need to not only adapt but evolve to survive the changing climate conditions. Temperature selection experiments were performed on Pseudomonas fluorescens to select for growth at lower temperatures. The P. fluorescens temperature selection experiment selected for cells that can grow at a new minimum temperature which is over 20˚C lower than the optimal growth temperature (25-30˚C). Previous experiments established the low end of P. fluorescens’s growth temperature as 4˚C. The genomes of the newly selected and reference st

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E. H., Achbani, S. Sadik, R. El Kahkahi, A. Benbouazza, and H. Mazouz. "First Report on Pseudomonas marginalis Bacterium Causing Soft Rot of Onion in Morocco." Atlas Journal of Biology 3, no. 2 (2017): 218–23. http://dx.doi.org/10.5147/ajb.v3i2.33.

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Pseudomonas marginalis is an important postharvest pathogen causing soft rot in a wide variety of harvested fruits and vegetables. Isolated strains from rotten onion bulbs based on morphological characteristics, were tested for pathogenecity on tobacco. Pathogenic strains underwent a biochemical test which detected the presence of P. marginalis. Symptoms were reproduced by inoculating this species to onion leaves and bulbs. Based on ARN16S sequencing, reported associated species (Pantoea agglomerans, Pseudomonas fluorescens, Klebsiella oxytoca) were also confirmed. This study reports for the f

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Ahmed, Ahmed, and Muntaha Hassan. "Monitoring the prevalence of Pseudomonas fluorescens as a spoilage indicator in cow raw milk, teat surfaces and milk tanks." Open Veterinary Journal 14, no. 8 (2024): 1983. http://dx.doi.org/10.5455/ovj.2024.v14.i8.27.

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Background: Milk and its products are very sensitive to spoilage if they are kept under unsuitable conditions which may provide favorable circumstances for the growth of specific spoilage organisms, Pseudomonas fluorescens accounted as the most dominant indicator for milk spoilage. Aim: This study highlights to monitor the prevalence of P. fluorescens as a spoilage indicator organism in cow raw milk and its contact surfaces represented by teat surfaces and milk tanks in Nineveh province. Methods: A total of 150 samples from cows' raw milk, teat surfaces and milk tank swabs were collected from

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Bodilis, Josselin, and Sylvie Barray. "Molecular evolution of the major outer-membrane protein gene (oprF) of Pseudomonas." Microbiology 152, no. 4 (2006): 1075–88. http://dx.doi.org/10.1099/mic.0.28656-0.

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The major outer-membrane protein of Pseudomonas, OprF, is multifunctional. It is a non-specific porin that plays a role in maintenance of cell shape, in growth in a low-osmolarity environment, and in adhesion to various supports or molecules. OprF has been studied extensively for its utility as a vaccine component, its role in antimicrobial drug resistance, and its porin function. The authors have previously shown important differences between the OprF and 16S rDNA phylogenies: Pseudomonas fluorescens isolates split into two quite separate clusters, probably according to their ecological niche

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Drancourt, Michel, Claude Bollet, Antoine Carlioz, Rolland Martelin, Jean-Pierre Gayral, and Didier Raoult. "16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates." Journal of Clinical Microbiology 38, no. 10 (2000): 3623–30. http://dx.doi.org/10.1128/jcm.38.10.3623-3630.2000.

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Some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16S ribosomal DNA (rDNA)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. However, as yet, the usefulness of 16S rDNA sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. In this study, we evaluated the utility of 16S rDNA sequencing as a means to identify a collection of 177 such isolates obtained from environmen

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Park, Ji Eun, Young Ree Kim, Sung Ha Kang, Sun Hyung Kim, Jae-Wang Kim, and Keun Hwa Lee. "Subcutaneous Mycetoma of Pseudallescheria boydii complexes Revealed by 18S rDNA sequencing." Journal of Medicine and Life Science 10, no. 1 (2013): 50–53. http://dx.doi.org/10.22730/jmls.2013.10.1.50.

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Pseudallescheria ellipsoidea, one of the P. boydii complexes, is a ubiquitously isolated saprophytic fungus from soil, poultry,cattle, sewage, and polluted water. It is known to cause various diseases such as subcutaneous mycetoma in immunocompetentindividuals and sepsis in immunocompromized patients. We experienced a case of cutaneous P. ellipsoidea infection in a 79-year-old man. He was taking medication for diabetes mellitus and heart disease and visited our dermatologic outpatient clinicwith violet-colored, nontender, fluctuating, keratotic, conglomerated plaques on the right forearm. Skin

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Huang, Danqiong, Guiping Yan, Neil Gudmestad, et al. "Molecular Characterization and Identification of Stubby Root Nematode Species From Multiple States in the United States." Plant Disease 102, no. 11 (2018): 2101–11. http://dx.doi.org/10.1094/pdis-10-17-1668-re.

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Stubby root nematodes (SRN) are important plant parasites infecting many crops and widely distributed in many regions of the United States. SRN transmit Tobacco rattle virus, which causes potato corky ringspot disease, thereby having a significant economic impact on the potato industry. In 2015 to 2017, 184 soil samples and 16 nematode suspensions from North Dakota, Minnesota, Idaho, Oregon, Washington, South Carolina, North Carolina, and Florida were assayed for the presence of SRN. SRN were found in 106 soil samples with population densities of 10 to 320 SRN per 200 g of soil and in eight of

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Vidigal, Pedro Marcus Pereira, João Mattos Brum, Maryoris Elisa Soto Lopez, Hilário Cuquetto Mantovani, and Humberto Moreira Hungaro. "Pijolavirus UFJF_PfSW6 Infection in Pseudomonas fluorescens Induces a Prophage Belonging to a Novel Genus in Peduoviridae Family." DNA 4, no. 4 (2024): 519–29. https://doi.org/10.3390/dna4040035.

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Background/Objectives: This study explores the genome sequencing data from the infection of Pseudomonas fluorescens UFV 041 by the bacteriophage Pijolavirus UFJF_PfSW6, aiming to identify and characterize prophages induced in the host bacterium during the infection. Methods: Scaffolds from sequencing data were analyzed, and reads were mapped to identify potential prophages using phage-to-host coverage metrics. The putative prophage scaffold was annotated, taxonomically classified, and its integration in the host bacterium was verified by PCR amplification of two target genes. We also tested wh

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de Bruijn, I., M. J. D. de Kock, P. de Waard, T. A. van Beek, and J. M. Raaijmakers. "Massetolide A Biosynthesis in Pseudomonas fluorescens." Journal of Bacteriology 190, no. 8 (2007): 2777–89. http://dx.doi.org/10.1128/jb.01563-07.

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ABSTRACT Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes, designated massA, massB, and massC, spanning approximately 30 kb. Prediction of the nature and configuration of the amino acids by in silico analysis of adenylation and condensation domains of the NRPSs was consistent with the chemically determined structure of the pepti

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Deyett, Elizabeth, M. Caroline Roper, Paul Ruegger, Jiue-In Yang, James Borneman, and Philippe E. Rolshausen. "Microbial Landscape of the Grapevine Endosphere in the Context of Pierce’s Disease." Phytobiomes Journal 1, no. 3 (2017): 138–49. http://dx.doi.org/10.1094/pbiomes-08-17-0033-r.

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Microbial community structure and composition in the plant vascular endosphere has not been studied extensively especially in the context of vascular diseases. Pierce’s disease (PD) of grapevine is caused by Xylella fastidiosa, a xylem-limited bacterium. In PD-impacted vineyards, there are observations of vines that remain asymptomatic despite being under high disease pressure. Because these vines are clonal, we hypothesized that the microbial community inhabiting the grapevine vascular endosphere is a major contributor to the disease escape phenotype. We used a next-generation sequencing Illu

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Khan, Mohd Salman, and Mohammad Saghir Khan. "Effect of Plant Beneficial Rhizobacteria on Growth and Yield of Wheat (Triticum aestivum L.) Grown in a Sandy Clay Loam Soil under Pot Experiment." Journal of Experimental Agriculture International 46, no. 12 (2024): 719–31. https://doi.org/10.9734/jeai/2024/v46i123181.

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Excessive and injudicious application of agrochemicals to optimize crop production has shown detrimental effects on microbial composition and function, soil-plant ecosystems, and human health via food chain. The microbiological strategies (phosphorus solubilization and ammonium production etc.), reduce the chemical dependency and mitigate environmental and human health risks. This study aimed to isolate and characterize plant beneficial bacteria from maize rhizospheres and asses their impact on wheat performance in pot trials. Cultures were characterized by morphological and biochemical assays

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Ahn, Jung Hoon, Jae Gu Pan, and Joon Shick Rhee. "Identification of the tliDEF ABC Transporter Specific for Lipase in Pseudomonas fluorescensSIK W1." Journal of Bacteriology 181, no. 6 (1999): 1847–52. http://dx.doi.org/10.1128/jb.181.6.1847-1852.1999.

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ABSTRACT Pseudomonas fluorescens, a gram-negative psychrotrophic bacterium, secretes a thermostable lipase into the extracellular medium. In our previous study, the lipase of P. fluorescensSIK W1 was cloned and expressed in Escherichia coli, but it accumulated as inactive inclusion bodies. Amino acid sequence analysis of the lipase revealed a potential C-terminal targeting sequence recognized by the ATP-binding cassette (ABC) transporter. The genetic loci around the lipase gene were searched, and a secretory gene was identified. Nucleotide sequencing of an 8.5-kb DNA fragment revealed three co

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De Vos, Daniel, Christiane Bouton, Alain Sarniguet, Paul De Vos, Marc Vauterin, and Pierre Cornelis. "Sequence Diversity of the oprI Gene, Coding for Major Outer Membrane Lipoprotein I, among rRNA Group I Pseudomonads." Journal of Bacteriology 180, no. 24 (1998): 6551–56. http://dx.doi.org/10.1128/jb.180.24.6551-6556.1998.

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ABSTRACT The sequence of oprI, the gene coding for the major outer membrane lipoprotein I, was determined by PCR sequencing for representatives of 17 species of rRNA group I pseudomonads, with a special emphasis on Pseudomonas aeruginosa andPseudomonas fluorescens. Within the P. aeruginosa species, oprI sequences for 25 independent isolates were found to be identical, except for one silent substitution at position 96. The oprI sequences diverged more for the other rRNA group I pseudomonads (85 to 91% similarity with P. aeruginosa oprI). An accumulation of silent and also (but to a much lesser

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Dinh Duy, Vu, Tran Thi Viet Thanh, Phan Ke Loc, et al. "Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An." Vietnam Journal of Biotechnology 18, no. 1 (2020): 75–85. http://dx.doi.org/10.15625/1811-4989/18/1/15267.

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DNA barcoding is a useful tool for species identification using standardized genomic DNA fragments. We used DNA barcodes (ITS-rDNA and matK gene) to explore Panax (32 samples collected from Phu Xai Lai Leng mountain and 19 samples collected from medicinal nursery of TH), and to investigate the phylogenetic taxonomy of Panax. In this study, the PCR success rate for ITS-rDNA and matK region was 100%. The success rate of bidirectional sequencing of PCR product was 100% of ITS-rDNA and matK region with length of 616 bp, 1433 bp, respectively. All 32 samples (Panax TB) of Phu Xai Lai Leng have a cl

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Zhou, Cheng, Zhongyou Ma, Xiaoming Lu, Lin Zhu, and Congsheng Yan. "Pseudomonas fluorescens MZ05 Enhances Resistance against Setosphaeria turcica by Mediating Benzoxazinoid Metabolism in the Maize Inbred Line Anke35." Agriculture 10, no. 2 (2020): 32. http://dx.doi.org/10.3390/agriculture10020032.

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Beneficial rhizobacteria can inhibit foliar pathogen infection by activation of defense responses, yet it the mechanisms of rhizobacteria-induced disease resistance remain largely unknown. Here, inoculation of susceptible maize plants with Pseudomonas fluorescens MZ05 significantly reduced disease occurrence caused by the leaf pathogen Setosphaeria turcica. Gene expression profiles of MZ05-inoculated plants were investigated by RNA-sequencing analyses, showing that several differentially expressed genes were positively associated with the metabolic processes of benzoxazinoids. Accordantly, the

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Landa, Blanca B., Dmitri M. Mavrodi, Linda S. Thomashow, and David M. Weller. "Interactions Between Strains of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens in the Rhizosphere of Wheat." Phytopathology® 93, no. 8 (2003): 982–94. http://dx.doi.org/10.1094/phyto.2003.93.8.982.

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Strains of fluorescent Pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. The genotypic diversity that exists among 2,4-DAPG producers can be exploited to improve rhizosphere competence and biocontrol activity. Knowing that D-genotype 2,4-DAPG-producing strains are enriched in some take-all decline soils and that P. fluorescens Q8r1-96, a representative D-genotype strain, as defined by whole-cell repetitive sequence-based polymerase chain reaction (rep-PCR) with the BOX

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Tynkevich, Y. O., L. V. Kozub, and R. A. Volkov. "Organization and polymorphysm of 5S rDNA intergenic spacer of blackthorn (Prunus spinosa L.)." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 19, no. 1-2 (2021): 40–46. http://dx.doi.org/10.7124/visnyk.utgis.19.1-2.1439.

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Aim. The 5S rDNA repeated units consist of conserved regions encoding 5S rRNA and variable intergenic spacers (IGS). The IGS sequences are commonly used as molecular markers for low-ranking phylogenetic, phylogeographical and microevolutionary studies. However, this genomic region still remains undescribed for the waist majority of genera in the Rosaceae family. Here we present the first report of the IGS molecular organization and polymorphism for the widespread member of the Rosaceae family, Prunus spinosa. Methods. PCR amplification, cloning and sequencing of 5S rDNA, bioinformatics analysi

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Liu, Zerui, Yu Guo, Chuanxin Qin, Xiaohui Mu, and Jia Zhang. "High-Throughput Sequencing Analysis Revealed a Preference for Animal-Based Food in Purple Sea Urchins." Biology 13, no. 8 (2024): 623. http://dx.doi.org/10.3390/biology13080623.

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Sea urchins play an important role in marine ecosystems. Owing to limitations in previous research methods, there has been insufficient understanding of the food sources and ecological functional value of purple sea urchins, leading to considerable controversy regarding their functional positioning. We focused on Daya Bay as the research area, utilizing stable isotope technology and high-throughput sequencing of 16S rDNA and 18S rDNA to analyze sea urchins and their potential food sources in stone and algae areas. The results showed that the δ13C range of purple sea urchins in the stone area i

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Upadhyaya, Narayana M., Rohit Mago, Brian J. Staskawicz, Michael A. Ayliffe, Jeffrey G. Ellis, and Peter N. Dodds. "A Bacterial Type III Secretion Assay for Delivery of Fungal Effector Proteins into Wheat." Molecular Plant-Microbe Interactions® 27, no. 3 (2014): 255–64. http://dx.doi.org/10.1094/mpmi-07-13-0187-fi.

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Large numbers of candidate effectors from fungal pathogens are being identified through whole-genome sequencing and in planta expression studies. Although Agrobacterium-mediated transient expression has enabled high-throughput functional analysis of effectors in dicot plants, this assay is not effective in cereal leaves. Here, we show that a nonpathogenic Pseudomonas fluorescens engineered to express the type III secretion system (T3SS) of P. syringae and the wheat pathogen Xanthomonas translucens can deliver fusion proteins containing T3SS signals from P. syringae (AvrRpm1) and X. campestris

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Roquigny, Roxane, Amy Novinscak, Geneviève Léger, Nathan Marcoux, David L. Joly, and Martin Filion. "Deciphering the Rhizosphere and Geocaulosphere Microbiomes of Potato Following Inoculation with the Biocontrol Agent Pseudomonas fluorescens Strain LBUM223." Phytobiomes Journal 2, no. 2 (2018): 92–99. http://dx.doi.org/10.1094/pbiomes-03-18-0013-r.

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The phenazine-1-carboxylic acid (PCA)-producing Pseudomonas fluorescens strain LBUM223 shows biocontrol potential against Streptomyces scabies, which causes common scab of potato. To better characterize the impact of inoculating this specific biocontrol agent under field conditions, the microbiomes of the rhizosphere and the geocaulosphere of potato plants were characterized using next-generation sequencing. A single initial application or biweekly applications of LBUM223 were performed up to 11 weeks after planting. Rhizosphere and geocaulosphere soils (when potato tubers were produced) were

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