To see the other types of publications on this topic, follow the link: P. freudenreichii.
Journal articles on the topic 'P. freudenreichii'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'P. freudenreichii.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Dherbécourt, J., H. Falentin, J. Jardin, et al. "Identification of a Secreted Lipolytic Esterase in Propionibacterium freudenreichii, a Ripening Process Bacterium Involved in Emmental Cheese Lipolysis." Applied and Environmental Microbiology 76, no. 4 (2009): 1181–88. http://dx.doi.org/10.1128/aem.02453-09.
Full textAbstract:
ABSTRACT Lipolysis plays an important role in the formation of cheese flavor. In Emmental cheese, the main part of lipolysis has been associated with the presence of Propionibacterium freudenreichii, a species used as a ripening culture. Our aim was to identify the most probable lipolytic esterase(s) involved in cheese lipolysis by P. freudenreichii. Since cheese lipolysis mainly occurs during P. freudenreichii growth, we hypothesized that P. freudenreichii possesses secreted lipolytic esterase(s). For 12 putative esterase genes previously identified from the genome of P. freudenreichii CIRM1, the level of expression was quantified by real-time reverse transcriptase (RT)-PCR, and the subcellular localization of esterases was predicted in silico. The esterase activity in extracellular and intracellular extracts of P. freudenreichii was characterized by zymography, and the extracellular esterases were identified by mass spectrometry. Finally, the best candidate was overexpressed in the same strain. All of the 12 genes encoding putative esterases were expressed. Esterase PF#279 was predicted to be secreted in the medium, PF#774 to be surface exposed, and the 10 remaining putative esterases to be intracellular. Zymography revealed that esterase activities in culture supernatant differed from the ones detected in intracellular extracts. PF#279 was identified as the sole esterase present in culture supernatant. Transformed P. freudenreichii CIRM1 clones overexpressing PF#279 showed 5 to 8 times more lipolytic activity on milk fat than the wild-type strain. Combining in silico, biochemical, and genetic approaches, we showed that PF#279 is the sole secreted esterase in P. freudenreichii and is active on milk fat. Therefore, it is likely a key component in cheese lipolysis by P. freudenreichii.
2
Hervé, C., A. Coste, A. Rouault, J. M. Fraslin, and M. Gautier. "First Evidence of Lysogeny inPropionibacterium freudenreichii subsp.shermanii." Applied and Environmental Microbiology 67, no. 1 (2001): 231–38. http://dx.doi.org/10.1128/aem.67.1.231-238.2001.
Full textAbstract:
ABSTRACT Dairy propionic acid bacteria, particularly the speciesPropionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lysogeny of P. freudenreichii was determined by isometric bacteriophage type analysis. The genomic DNA of 76 strains were hybridized with the DNA of nine bacteriophages isolated from Swiss type cheeses, and the DNA of 25 strains exhibited strong hybridization. Three of these strains released bacteriophage particules following UV irradiation (254 nm) or treatment with low concentrations of mitomycin C. A prophage-cured derivative of P. freudenreichii was readily isolated and subsequently relysogenized. Lysogeny was therefore formally demonstrated in P. freudenreichii.
3
Brede, Dag Anders, Sheba Lothe, Zhian Salehian, Therese Faye, and Ingolf F. Nes. "Identification of the Propionicin F Bacteriocin Immunity Gene (pcfI) and Development of a Food-Grade Cloning System for Propionibacterium freudenreichii." Applied and Environmental Microbiology 73, no. 23 (2007): 7542–47. http://dx.doi.org/10.1128/aem.01023-07.
Full textAbstract:
ABSTRACT This report describes the first functional analysis of a bacteriocin immunity gene from Propionibacterium freudenreichii and its use as a selection marker for food-grade cloning. Cloning of the pcfI gene (previously orf5 [located as part of the pcfABC propionicin F operon]) rendered the sensitive host 1,000-fold more tolerant to the propionicin F bacteriocin. The physiochemical properties of the 127-residue large PcfI protein resemble those of membrane-bound immunity proteins from bacteriocin systems found in lactic acid bacteria. The high level of immunity conferred by pcfI allowed its use as a selection marker for plasmid transformation in P. freudenreichii. Electroporation of P. freudenreichii IFO12426 by use of the pcfI expression plasmid pSL102 and propionicin F selection (200 bacteriocin units/ml) yielded 107 transformants/μg DNA. The 2.7-kb P. freudenreichii food-grade cloning vector pSL104 consists of the pLME108 replicon, a multiple cloning site, and pcfI expressed from the constitutive PpampS promoter for selection. The pSL104 vector efficiently facilitated cloning of the propionicin T1 bacteriocin in P. freudenreichii. High-level propionicin T1 production (640 BU/ml) was obtained with the IFO12426 strain, and the food-grade propionicin T1 expression plasmid pSL106 was maintained by ∼91% of the cells over 25 generations in the absence of selection. To the best of our knowledge this is the first report of an efficient cloning system that facilitates the generation of food-grade recombinant P. freudenreichii strains.
4
Brede, Dag Anders, Therese Faye, Melanie Patricia Stierli, et al. "Heterologous Production of Antimicrobial Peptides in Propionibacterium freudenreichii." Applied and Environmental Microbiology 71, no. 12 (2005): 8077–84. http://dx.doi.org/10.1128/aem.71.12.8077-8084.2005.
Full textAbstract:
ABSTRACT Heterologous bacteriocin production in Propionibacterium freudenreichii is described. We developed an efficient system for DNA shuttling between Escherichia coli and P. freudenreichii using vector pAMT1. It is based on the P. freudenreichii rolling-circle replicating plasmid pLME108 and carries the cml(A)/cmx(A) chloramphenicol resistance marker. Introduction of the propionicin T1 structural gene (pctA) into pAMT1 under the control of the constitutive promoter (P4) yielded bacteriocin in amounts equal to those of the wild-type producer Propionibacterium thoenii 419. The P. freudenreichii clone showed propionicin T1 activity in coculture, killing 90% of sensitive bacteria within 48 h. The pamA gene from P. thoenii 419 encoding the protease-activated antimicrobial peptide (PAMP) was cloned and expressed in P. freudenreichii, resulting in secretion of the pro-PAMP protein. Like in the wild type, PAMP activation was dependent on externally added protease. Secretion of the antimicrobial peptide was obtained from a clone in which the pamA signal peptide and PAMP were fused in frame. The promoter region of pamA was identified by fusion of putative promoter fragments to the coding sequence of the pctA gene. The P4 and Ppamp promoters directed constitutive gene expression, and activity of both promoters was enhanced by elements upstream of the promoter core region.
5
Gaucher, Floriane, Valérie Gagnaire, Houem Rabah, et al. "Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii." Microorganisms 7, no. 10 (2019): 477. http://dx.doi.org/10.3390/microorganisms7100477.
Full textAbstract:
Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viability within powders. Compared to freeze-drying, spray drying constitutes the most productive and efficient, yet the most stressful process, imposing severe oxidative and thermal constraints. The aim of our study was to provide the tools in order to optimize the industrial production of dry P. freudenreichii. Bacterial adaptation is a well-known protective mechanism and may be used to improve bacterial tolerance towards technological stresses. However, the choice of bacterial adaptation type must consider industrial constraints. In this study, we combined (i) modulation of the growth medium composition, (ii) heat-adaptation, and (iii) osmoadaptation, in order to increase P. freudenreichii tolerance towards technological stresses, including thermal and oxidative constraints, using an experimental design. We further investigated optimal growth and adaptation conditions, by monitoring intracellular compatible solutes accumulation. Glucose addition, coupled to heat-adaptation, triggered accumulation of trehalose and of glycine betaine, which further provided high tolerance towards spray drying and storage. This work opens new perspectives for high quality and fast production of live propionibacteria at the industrial scale.
6
Lan, Annaïg, Aurélia Bruneau, Martine Bensaada, et al. "Increased induction of apoptosis by Propionibacterium freudenreichii TL133 in colonic mucosal crypts of human microbiota-associated rats treated with 1,2-dimethylhydrazine." British Journal of Nutrition 100, no. 6 (2008): 1251–59. http://dx.doi.org/10.1017/s0007114508978284.
Full textAbstract:
Propionibacterium freudenreichii, a food-grade bacterium able to kill colon cancer cell lines in vitro by apoptosis, may exert an anticarcinogenic effect in vivo. To assess this hypothesis, we administered daily 2 × 1010 colony-forming units (CFU) of P. freudenreichii TL133 to human microbiota-associated (HMA) rats for 18 d. Either saline or 1,2-dimethylhydrazine (DMH) was also administered on days 13 and 17 and rats were killed on day 19. The levels of apoptosis and proliferation in the mid and distal colon were assessed by terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) and proliferating cell nuclear antigen (PCNA) immunolabelling, respectively. The administration of P. freudenreichii TL133 significantly increased the number of apoptotic cells in DMH-treated rats compared to those given DMH only (P < 0·01). Furthermore, propionibacteria were able to decrease the proliferation index in the distal colon after treatment with DMH (P < 0·01). Conversely, propionibacteria alone did not exert such an effect on healthy colonic mucosa. P. freudenreichii TL133 thus facilitated the elimination of damaged cells by apoptosis in the rat colon after genotoxic insult and may play a protective role against colon cancer.
7
Deutsch, Stéphanie-Marie, Pierre Le Bivic, Christophe Hervé, et al. "Correlation of the Capsular Phenotype in Propionibacterium freudenreichii with the Level of Expression of gtf, a Unique Polysaccharide Synthase-Encoding Gene." Applied and Environmental Microbiology 76, no. 9 (2010): 2740–46. http://dx.doi.org/10.1128/aem.02591-09.
Full textAbstract:
ABSTRACT Many food-grade bacteria produce exopolysaccharides (EPS) that affect the texture of fermented food products and that may be involved in probiotic properties. Propionibacterium freudenreichii is a Gram-positive food-grade bacterium with reported probiotic capabilities that is widely used as starter in Swiss-type cheese. In this study, 68 strains of P. freudenreichii were screened for the β-glucan capsular phenotype by immunoagglutination with a specific antibody and for the presence of the gtf gene coding for polysaccharide synthase. All strains were positive for PCR amplification with gtf gene-specific primers, but the presence of β-glucan capsular EPS was detected for only 35% of the strains studied. Disruption of gtf in P. freudenreichii revealed that gtf is a unique gene involved in β-glucan capsular EPS production in P. freudenreichii. The gtf gene was transferred into and expressed in Lactococcus lactis, in which it conferred an agglutination-positive phenotype. Expression of the gtf gene was measured by performing quantitative reverse transcription-PCR assays with RNA from four capsular and three noncapsular strains. A positive correlation was found between the β-glucan capsular phenotype and gtf gene expression. Sequencing of the region upstream of the gtf open reading frame revealed the presence of an insertion element (IS element) in this upstream region in the four strains with the β-glucan capsular phenotype. The role of the IS element in the expression of neighboring genes and its impact on interstrain variability of the P. freudenreichii capsule phenotype remain to be elucidated.
8
Faye, Therese, Anita Åsebø, Zhian Salehian, Thor Langsrud, Ingolf F. Nes, and Dag Anders Brede. "Construction of a Reporter Vector System for In Vivo Analysis of Promoter Activity in Propionibacterium freudenreichii." Applied and Environmental Microbiology 74, no. 11 (2008): 3615–17. http://dx.doi.org/10.1128/aem.02870-07.
Full textAbstract:
ABSTRACT A β-galactosidase reporter system for the analysis of promoter elements in Propionibacterium freudenreichii was designed. The pTD210 in vivo reporter vector was constructed using a promoterless lacZ gene from Bifidobacterium longum cloned into the pAMT1 plasmid. The utility of the pTD210 reporter vector was demonstrated by an investigation of six predicted promoters in P. freudenreichii. The system produced accurate and reproducible measurements that facilitated both promoter identification and the quantification of promoter activities.
9
Rabah, Houem, Fillipe Luiz Rosa do Carmo, Rodrigo Dias de Oliveira Carvalho, et al. "Beneficial Propionibacteria within a Probiotic Emmental Cheese: Impact on Dextran Sodium Sulphate-Induced Colitis in Mice." Microorganisms 8, no. 3 (2020): 380. http://dx.doi.org/10.3390/microorganisms8030380.
Full textAbstract:
Backgrounds and Aims. Inflammatory Bowel Diseases (IBD), including Ulcerative Colitis (UC), coincide with alterations in the gut microbiota. Consumption of immunomodulatory strains of probiotic bacteria may induce or prolong remission in UC patients. Fermented foods, including cheeses, constitute major vectors for bacteria consumption. New evidences revealed anti-inflammatory effects in selected strains of Propionibacterium freudenreichii. We thus hypothesized that consumption of a functional cheese, fermented by such a strain, may exert a positive effect on IBD. Methods. We investigated the impact of cheese fermented by P. freudenreichii on gut inflammation. We developed an experimental single-strain cheese solely fermented by a selected immunomodulatory strain of P. freudenreichii, CIRM-BIA 129. We moreover produced, in industrial conditions, an Emmental cheese using the same strain, in combination with Lactobacillus delbrueckii CNRZ327 and Streptococcus thermophilus LMD-9, as starters. Consumption of both cheeses was investigated with respect to prevention of Dextran Sodium Sulphate (DSS)-induced colitis in mice. Results. Consumption of the single-strain experimental cheese, or of the industrial Emmental, both fermented by P. freudenreichii CIRM-BIA 129, reduced severity of subsequent DSS-induced colitis, weight loss, disease activity index and histological score. Both treatments, in a preventive way, reduced small bowel Immunoglobulin A (IgA) secretion, restored occludin gene expression and prevented induction of Tumor Necrosis Factor α (TNFα), Interferon γ (IFNγ) and Interleukin-17 (IL-17). Conclusions. A combination of immunomodulatory strains of starter bacteria can be used to manufacture an anti-inflammatory cheese, as revealed in an animal model of colitis. This opens new perspectives for personalised nutrition in the context of IBD.
10
Jore, J. P. M., N. van Luijk, R. G. M. Luiten, M. J. van der Werf, and P. H. Pouwels. "Efficient Transformation System forPropionibacterium freudenreichii Based on a Novel Vector." Applied and Environmental Microbiology 67, no. 2 (2001): 499–503. http://dx.doi.org/10.1128/aem.67.2.499-503.2001.
Full textAbstract:
ABSTRACT A 3.6-kb endogenous plasmid was isolated from aPropionibacterium freudenreichii strain and sequenced completely. Based on homologies with plasmids from other bacteria, notably a plasmid from Mycobacterium, a region harboring putative replicative functions was defined. Outside this region two restriction enzyme recognition sites were used for insertion of anEscherichia coli-specific replicon and an erythromycin resistance gene for selection in Propionibacterium. Hybrid vectors obtained in this way replicated in both E. coli andP. freudenreichii. Whereas electroporation of P. freudenreichii with vector DNA isolated from an E. coli transformant yielded 10 to 30 colonies per μg of DNA, use of vector DNA reisolated from a Propionibacteriumtransformant dramatically increased the efficiency of transformation (≥108 colonies per μg of DNA). It could be shown that restriction-modification was responsible for this effect. The high efficiency of the system described here permitted successful transformation of Propionibacterium with DNA ligation mixtures.
11
Ding, S., S. J. Meale, A. Y. Alazzeh, et al. "Effect of Propionibacterium freudenreichii in diets containing rapeseed or flaxseed oil on in vitro ruminal fermentation, methane production and fatty acid biohydrogenation." Animal Production Science 57, no. 10 (2017): 2051. http://dx.doi.org/10.1071/an15878.
Full textAbstract:
The objectives of the present study were to determine the effect of inoculating Propionibacterium freudenreichii subsp. shermanii ATCC 8262 (1 × 109 colony-forming units per vial) in a barley silage-based diet supplemented with flaxseed oil or rapeseed oil (60 g/kg DM), on in vitro proportions and yield of volatile fatty acids, methane production and fatty acid (FA) biohydrogenation. Total volatile fatty acid production (mM) and proportions of individual FAs were not affected (P ≥ 0.10) by P. freudenreichii. Similarly, propionibacteria had little impact on FA biohydrogenation, resulting only in an increased accumulation (P < 0.01) of C18:1 cis-15 (g/kg total FA) at 6 h of incubation, compared with the control (CON). Compared with the CON, an increased (P < 0.01) accumulation of vaccenic acid was observed at 48 h in all oil-containing treatments, regardless of the oil type. Similarly, the apparent biohydrogenation of flaxseed oil resulted in an increased (P ≤ 0.04) accumulation of conjugated linoleic acid cis-9, trans-11, compared with all other treatments. Additionally, flaxseed oil produced a greater (P ≤ 0.01) accumulation of beneficial biohydrogenation intermediates (C18:2 trans-11, cis-15; C18:1 cis-15 and vaccenic acid), reflecting its ability to produce a more desirable FA profile than that of rapeseed oil or CON. The inability of P. freudenreichii subsp. shermanii ATCC 8262 to alter ruminal fermentation in a manner that lowered methane production, along with only minor effects on FA profiles through biohydrogenation, suggests that the biological activity of this strain was not realised under in vitro batch-culture conditions.
12
Zahed, Omid, Kianoush Khosravi-Darani, S. Amir Mortazavian Farsani, and Abdoreza Mohammadi. "Bacterial conjugated linoleic acid bio-fortification of synbiotic yogurts using Propionibacterium freudenreichii as adjunct culture." Italian Journal of Food Science 33, SP1 (2021): 1–11. http://dx.doi.org/10.15586/ijfs.v33isp1.1961.
Full textAbstract:
In this study, Propionibacterium freudenreichii was used for in situ production of conjugated linoleic acid (CLA) in yogurt. Firstly, effects of process variables, including strain type, percentage of milk fat, percentage of inoculum, quantity of sunflower oil, concentration of inulin, temperature of fermentation and time of storage at 4°C, on production of CLA by Propionibacterium freudenreichii were investigated using screening method of the Plackett–Burman design. Then optimisation of CLA production process was conducted using three major factors of milk fat percentage, inulin concentration and storage time at 4°C using central composite design. Analysis of variance established that the models were highly significant (P ? 0.05). The model demonstrated that the production of CLA was affected by these three factors. Optimised CLA production by Propionibacterium freudenreichii ssp. shermanii in yogurts was achieved after 17 days of storage at 4°C in skim-milk containing 1.75% (w/w) fat and 2.25% (w/v) inulin as prebiotic. Reconfirmation test established that at the highlighted optimum conditions, the highest concentration of produced CLA was 6.4 mg g–1 lipid in yogurt, which is a 256% increase in total CLA production, compared with control samples. Results demonstrated that Propionibacterium freudenreichii ssp. shermanii not only leads to production of synbiotic yogurts containing inulin but also increases CLA production in yogurts.
13
Zahed, Omid, Kianoush Khosravi-Darani, S. Amir Mortazavian Farsani, and Abdoreza Mohammadi. "Bacterial conjugated linoleic acid bio-fortification of synbiotic yogurts using Propionibacterium freudenreichii as adjunct culture." Italian Journal of Food Science 33, SP1 (2021): 1–11. http://dx.doi.org/10.15586/ijfs.v33isp1.1961.
Full textAbstract:
In this study, Propionibacterium freudenreichii was used for in situ production of conjugated linoleic acid (CLA) in yogurt. Firstly, effects of process variables, including strain type, percentage of milk fat, percentage of inoculum, quantity of sunflower oil, concentration of inulin, temperature of fermentation and time of storage at 4°C, on production of CLA by Propionibacterium freudenreichii were investigated using screening method of the Plackett–Burman design. Then optimisation of CLA production process was conducted using three major factors of milk fat percentage, inulin concentration and storage time at 4°C using central composite design. Analysis of variance established that the models were highly significant (P ? 0.05). The model demonstrated that the production of CLA was affected by these three factors. Optimised CLA production by Propionibacterium freudenreichii ssp. shermanii in yogurts was achieved after 17 days of storage at 4°C in skim-milk containing 1.75% (w/w) fat and 2.25% (w/v) inulin as prebiotic. Reconfirmation test established that at the highlighted optimum conditions, the highest concentration of produced CLA was 6.4 mg g–1 lipid in yogurt, which is a 256% increase in total CLA production, compared with control samples. Results demonstrated that Propionibacterium freudenreichii ssp. shermanii not only leads to production of synbiotic yogurts containing inulin but also increases CLA production in yogurts.
14
Kiatpapan, Pornpimon, Yoshiteru Hashimoto, Hisako Nakamura, et al. "Characterization of pRGO1, a Plasmid from Propionibacterium acidipropionici, and Its Use for Development of a Host-Vector System in Propionibacteria." Applied and Environmental Microbiology 66, no. 11 (2000): 4688–95. http://dx.doi.org/10.1128/aem.66.11.4688-4695.2000.
Full textAbstract:
ABSTRACT The complete nucleotide sequence of pRGO1, a cryptic plasmid fromPropionibacterium acidipropionici E214, was determined. pRGO1 is 6,868 bp long, and its G+C content is 65.0%. Frame analysis of the sequence revealed six open reading frames, which were designated Orf1 to Orf6. The deduced amino acid sequences of Orf1 and Orf2 showed extensive similarities to an initiator of plasmid replication, the Rep protein, of various plasmids of gram-positive bacteria. The amino acid sequence of the putative translation product of orf3 exhibited a high degree of similarity to the amino acid sequences of DNA invertase in several bacteria. For the putative translation products of orf4,orf5, and orf6, on the other hand, no homologous sequences were found. The function of these open reading frames was studied by deletion analysis. A shuttle vector, pPK705, was constructed for shuttling between Escherichia coli and a Propionibacterium strain containingorf1 (repA), orf2(repB), orf5, and orf6 from pRGO1, pUC18, and the hygromycin B-resistant gene as a drug marker. Shuttle vector pPK705 successfully transformed Propionibacterium freudenreichii subsp. shermanii IFO12426 by electroporation at an efficiency of 8 × 106 CFU/μg of DNA under optimized conditions. Transformation of various species of propionibacteria with pPK705 was also performed at efficiencies of about 104 to 107 CFU/μg of DNA. The vector was stably maintained in strains of P. freudenreichiisubsp. shermanii, P. freudenreichii, P. pentosaceum, and P. freudenreichii subsp.freudenreichii grown under nonselective conditions. Successful manipulation of a host-vector system in propionibacteria should facilitate genetic studies and lead to creation of genes that are useful industrially.
15
Šalaková, A., J. Bártová, J. Drbohlav, and P. Roubal. "Contribution to the immunomodulatory characteristics of probiotic bacteria." Czech Journal of Food Sciences 29, Special Issue (2012): S36—S41. http://dx.doi.org/10.17221/303/2011-cjfs.
Full textAbstract:
We monitored the impact of selected probiotic strains on the human immune system. 13 strains of lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus helveticus, Enterococcus faecium, Streptococcus thermophilus, and Propionibacterium freudenreichii subsp. shermanii), used in the production of functional foods, were tested from the point of view of their ability to stimulate a range of cytokines. The selected cytokines have regulatory properties; they affect the progression of inflammation and the inhibition of inflammatory response, and they play a role in humoral immunity, allergies, and cell-mediated immunity. The tested strains showed a specific interaction with the immune system. It was found that the P. freudenreichii strain showed significant levels of IFN gamma cytokine.
16
Meale, S. J., S. Ding, M. L. He, et al. "Effect ofPropionibacterium freudenreichiion ruminal fermentation patterns, methane production and lipid biohydrogenation of beef finishing diets containing flaxseed oil in a rumen simulation technique." Canadian Journal of Animal Science 94, no. 4 (2014): 685–95. http://dx.doi.org/10.4141/cjas-2014-051.
Full textAbstract:
Meale, S. J., Ding, S., He, M. L., Dugan, M. E. R., Ribeiro Jr. G. O., Alazzeh, A. Y., Holo, H., Harstad, O. M., McAllister, T. A. and Chaves, A. V. 2014. Effect of Propionibacterium freudenreichii on ruminal fermentation patterns, methane production and lipid biohydrogenation of beef finishing diets containing flaxseed oil in a rumen simulation technique. Can. J. Anim. Sci. 94: 685–695. The objectives of this study were to examine the effects of Propionibacterium freudenreichii (strain T54; PB) and flaxseed oil (FO) in a total mixed ration on ruminal fermentation, CH4production and fatty acid biohydrogenation in two artificial rumens (RUSITEC). The experiment consisted of 8 d of adaptation and 12 d of sample collection with four replicate fermenters per treatment. Treatments were: (1) CON; (2) PB; (3) FO (60 g kg−1DM with autoclaved PB); (4) FOPB (60 g kg−1DM with PB). Disappearance of DM (g kg−1DM) and gas production (mL g−1DM) were not affected by treatment (P>0.05). Inclusion of FOPB increased (P=0.01) total volatile fatty acid (VFA) production (mmol d−1), compared with CON and PB. The acetate:propionate ratio was reduced (P<0.001) in all treatments, compared with CON. Methane production (mL g−1DM or mL g−1DMD) was lowest (P<0.001) with PB (27.1%); however, FO (14.3%) and FOPB (19.3%) also reduced CH4compared with CON. Fatty acid profiles for PB were similar (P>0.05) to CON for most fatty acids. Concentrations of 18:3n-3 were greater (P<0.001) in FO and FOPB in both digesta and effluent, compared with CON. Propionibacterium freudenreichii had very little effect on ruminal biohydrogenation, but reduced CH4production under the current conditions as a result of increasing propionate production.
17
DARILMAZ, DERYA ONAL, and YESIM GUMUSTEKIN. "Research on Some Factors Influencing Acid and Exopolysaccharide Produced by Dairy Propionibacterium Strains Isolated from Traditional Homemade Turkish Cheeses." Journal of Food Protection 75, no. 5 (2012): 918–26. http://dx.doi.org/10.4315/0362-028x.jfp-11-510.
Full textAbstract:
In this study, a total of 32 isolated strains and 5 reference strains of dairy propionibacteria were analyzed for acid and exopolysaccharide (EPS) production in skim milk and yeast extract–lactate broth (YEL) media in order to investigate the physiological background and preservative role of acid and EPS. The effects of final culture pH and optical density on acid and EPS production were also determined. On average, all strains produced more acid and reached lower final pH values in skim milk than in YEL medium. While the correlations obtained between the acid produced by propionibacterium strains and their final culture pH in skim milk medium were significant (P &lt; 0.01), no correlations were found between optical density, final pH, and produced acid in YEL medium. Sixteen isolated and five reference strains of propionibacteria were tested further for the ability to produce propionic and acetic acids. On average, Propionibacterium freudenreichii subsp. shermanii and P. freudenreichii subsp. freudenreichii strains produced higher amounts of propionic and acetic acids than did Propionibacterium jensenii in YEL medium. The acid produced by these strains may be used as a preservative in the food industry for replacement or reduction of the increasing use of chemical additives. The EPS production by propionibacterium strains during growth in YEL medium was 72 to 168 mg/liter, while in skim milk it was 94 to 359 mg/liter. The monomer compositions of the EPSs formed by the six selected dairy propionibacteria strains were analyzed. The EPSs may have applications as food grade additives and viscosity-stabilizing agents.
18
Vyas, D., A. Alazzeh, S. M. McGinn, et al. "Enteric methane emissions in response to ruminal inoculation of Propionibacterium strains in beef cattle fed a mixed diet." Animal Production Science 56, no. 7 (2016): 1035. http://dx.doi.org/10.1071/an14801.
Full textAbstract:
The objective of this study was to test the efficacy of Propionibacterium strains to mitigate enteric methane (CH4) emissions in beef heifers fed a mixed diet. An experiment was conducted with 16 ruminally cannulated beef heifers fed a basal diet consisting of 60 : 40 barley silage : barley grain (DM basis). Treatments included: (1) Control, (2) Propionibacterium freudenreichii T114, (3) P. thoenii T159, and (4) P. freudenreichii T54. Strains (1 × 1011 colony forming units) were administered daily directly into the rumen before feeding. No treatment effects were observed for DM intake (P = 0.90), mean ruminal pH (P = 0.50) and total volatile fatty acids (P = 0.44). However, compared with the Control, proportions of individual volatile fatty acids changed with acetate being less with Propionibacterium T159 (P = 0.02), whereas ruminal isobutyrate (P < 0.01) and acetate : propionate ratio (P = 0.04) were greater with Propionibacterium T114. Total daily enteric CH4 production averaged 188 g/day and was not affected by Propionbacterium strains (P = 0.51). Methane yield averaged 22 g/kg of DMI intake and tended to be greater with Propionibacterium strains (P = 0.08). The relative abundance of total Propionibacteria was greater with the inoculation of Propionibacterium T159 relative to the Control heifers (P = 0.04). In conclusion, inoculation of Propionibacterium T159 decreased ruminal acetate proportion and Propionibacterium T114 increased acetate : propionate ratio. However, inoculated strains failed to lower total CH4 emissions possibly due to the inability of Propionibacterium strains to elevate ruminal propionate concentrations.
19
YOUNTS-DAHL, S. M., M. L. GALYEAN, G. H. LONERAGAN, N. A. ELAM, and M. M. BRASHEARS. "Dietary Supplementation with Lactobacillus- and Propionibacterium-Based Direct-Fed Microbials and Prevalence of Escherichia coli O157 in Beef Feedlot Cattle and on Hides at Harvest." Journal of Food Protection 67, no. 5 (2004): 889–93. http://dx.doi.org/10.4315/0362-028x-67.5.889.
Full textAbstract:
The objective of this study was to describe the prevalence of Escherichia coli O157 in the feces and on the hides of finishing beef cattle fed a standard diet and those fed diets supplemented with direct-fed microbials. Two hundred forty steers received one of four treatments throughout the feeding period: (i) control: no added microbials; (ii) HNP51: high dose of Lactobacillus acidophilus strain NP 51 (109 CFU per steer daily) and Propionibacterium freudenreichii (109 CFU per steer daily); (iii) HNP51+45: high dose of NP 51 (109 CFU per steer daily), P. freudenreichii (109 CFU per steer daily), and L. acidophilus NP 45 (106 CFU per steer daily); or (iv) LNP51+ 45: low dose of NP 51 (106 CFU per steer daily), P. freudenreichii (109 CFU per steer daily), and NP 45 (106 CFU per steer daily). Samples were collected from each animal and analyzed for the presence of E. coli O157 using immunomagnetic separation methods on day 0 (feces), 7 days before harvest (feces), and at harvest (feces and hide). At the end of the feeding period, cattle receiving HNP51 were 57% less likely to shed detectable E. coli O157 in their feces than were the controls (P &lt; 0.01). For animals receiving HNP51+ 45 and LNP51+ 45, fecal prevalence did not differ from that of the controls. The prevalence of positive hide samples was least among cattle receiving HNP51+ 45 (3.3%); these animals were 79% less likely (P &lt; 0.06) to have a positive hide sample than were the controls (prevalence = 13.8%). There was poor agreement of the culture results between fecal and hide samples collected from the same animal (κ = 0.08; confidence interval = −0.05 to 0.2). Cattle supplemented with a high dose of NP 51 had reduced E. coli O157 prevalence in both fecal and hide samples, indicating that this treatment may be an efficacious preharvest intervention strategy.
20
Myllyluoma, E., A. M. Ahonen, R. Korpela, H. Vapaatalo, and E. Kankuri. "Effects of Multispecies Probiotic Combination on Helicobacter pylori Infection In Vitro." Clinical and Vaccine Immunology 15, no. 9 (2008): 1472–82. http://dx.doi.org/10.1128/cvi.00080-08.
Full textAbstract:
ABSTRACT Probiotic bacteria alleviate many gastrointestinal symptoms, but the current trend of combining bacteria for additional benefit may make their effects more complex. We characterize four probiotics and their combination in terms of pathogen adhesion, barrier function, cell death, and inflammatory response in Helicobacter pylori-infected epithelial cells. H. pylori-infected Caco-2 cells were pretreated with Lactobacillus rhamnosus GG, Lactobacillus rhamnosus Lc705, Propionibacterium freudenreichii subsp. shermanii Js, Bifidobacterium breve Bb99, or all four organisms in combination. We evaluated the adhesion of H. pylori by in situ immunofluorescence; epithelial barrier function by measurement of transepithelial resistance; apoptosis by measurement of caspase 3 activation; cell membrane leakage by measurement of lactate dehydrogenase release; and inflammation by measurement of interleukin-8 (IL-8), IL-10, prostaglandin E2 (PGE2), and leukotriene B4 (LTB4) release. All probiotics inhibited H. pylori adhesion. L. rhamnosus GG, L. rhamnosus Lc705, P. freudenreichii subsp. shermanii Js, and the combination inhibited H. pylori-induced cell membrane leakage. L. rhamnosus GG, L. rhamnosus Lc705, and the combination initially improved epithelial barrier function but increased the H. pylori-induced barrier deterioration after incubation for 24 to 42 h. L. rhamnosus GG, L. rhamnosus Lc705, and P. freudenreichii subsp. shermanii Js inhibited H. pylori-induced IL-8 release, whereas L. rhamnosus GG, L. rhamnosus Lc705, and B. breve Bb99 suppressed PGE2 release. None of these anti-inflammatory effects persisted when the probiotics were used in combination. The combination thus increased the levels of IL-8, PGE2, and LTB4 released from H. pylori-infected epithelial cells. The proinflammatory actions of the individual components dominated the anti-inflammatory effects when the probiotic bacteria were used in combination. Our results stress that the therapeutic response can be optimized if probiotic strains are characterized before they are used in combination.
21
Brede, Dag Anders, Therese Faye, Ola Johnsborg, Inger Ødegård, Ingolf F. Nes, and Helge Holo. "Molecular and Genetic Characterization of Propionicin F, a Bacteriocin from Propionibacterium freudenreichii." Applied and Environmental Microbiology 70, no. 12 (2004): 7303–10. http://dx.doi.org/10.1128/aem.70.12.7303-7310.2004.
Full textAbstract:
ABSTRACT This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to bacteriocins. The mass of the purified bacteriocin was determined by mass spectrometry, and the N-terminal amino acid sequence was determined by Edman degradation. Sequencing of pcfA, the bacteriocin structural gene, revealed that propionicin F corresponds to a 43-amino-acid peptide in the central part of a 255-amino-acid open reading frame, suggesting that mature propionicin F is excised from the probacteriocin by N- and C-terminal proteolytic modifications. DNA sequencing and Northern blot hybridizations revealed that pcfA is cotranscribed with genes encoding a putative proline peptidase and a protein from the radical S-adenosylmethionine family. A gene encoding an ABC transporter was also identified in close proximity to the bacteriocin structural gene. The potential role of these genes in propionicin F maturation and secretion is discussed.
22
Massoud, Ramona, Kianoush Khosravi-Darani, Milad Golshahi, Sara Sohrabvandi, and Amir Mohammad Mortazavian. "Assessment of Process Variables on Vitamin B12 Production in Fermented Dairy Product Including Propionic Acid." Current Nutrition & Food Science 16, no. 2 (2020): 155–61. http://dx.doi.org/10.2174/1573401315666181204105601.
Full textAbstract:
The fermented dairy products produced by various microorganism’s activity provide valuable nutrients for human. Fermentation affects the physicochemical and organoleptic characteristics of foods as well as human health. In the present review, we describe the production of vitamin B12 in a fermented dairy product by Propionibacterium species. The effect of the process variables on vitamin B12 production in fermented dairy products e.g. pH, temperature, different carbon and nitrogen sources as well as the type and size of inoculum, the fermentation time and fermentation strategy, etc. are discussed. Finally, fermentation strategy, inoculum preparation of Propionibacterium freudenreichii subsp. Shermanii, methods of determining biomass and Vitamin B12 concentration are reviewed and their effects on vitamin B12 production are mentioned. Propionibacteria are so popular due to their unique characteristics such as being safe, convenient and economical. Among all the highest efficiency was observed by P. freudenreichii. To achieve this purpose, some appropriate situations need to be considered. The best carbon source for this bacterium to produce biomass is lactate, the suitable pH for growth of the Propionibacterium species is in the range of 6 to 7 and the fed-batch is most preferable for vitamin B12 production.
23
Benz, Marcus, Bernhard Schink, and Andreas Brune. "Humic Acid Reduction by Propionibacterium freudenreichii and Other Fermenting Bacteria." Applied and Environmental Microbiology 64, no. 11 (1998): 4507–12. http://dx.doi.org/10.1128/aem.64.11.4507-4512.1998.
Full textAbstract:
ABSTRACT Iron-reducing bacteria have been reported to reduce humic acids and low-molecular-weight quinones with electrons from acetate or hydrogen oxidation. Due to the rapid chemical reaction of amorphous ferric iron with the reduced reaction products, humic acids and low-molecular-weight redox mediators may play an important role in biological iron reduction. Since many anaerobic bacteria that are not able to reduce amorphous ferric iron directly are known to transfer electrons to other external acceptors, such as ferricyanide, 2,6-anthraquinone disulfonate (AQDS), or molecular oxygen, we tested several physiologically different species of fermenting bacteria to determine their abilities to reduce humic acids.Propionibacterium freudenreichii, Lactococcus lactis, and Enterococcus cecorum all shifted their fermentation patterns towards more oxidized products when humic acids were present; P. freudenreichii even oxidized propionate to acetate under these conditions. When amorphous ferric iron was added to reoxidize the electron acceptor, humic acids were found to be equally effective when they were added in substoichiometric amounts. These findings indicate that in addition to iron-reducing bacteria, fermenting bacteria are also capable of channeling electrons from anaerobic oxidations via humic acids towards iron reduction. This information needs to be considered in future studies of electron flow in soils and sediments.
24
Lee, Jeong-Hoon, Mi-Jung Choi, Koo-Chun Chung, and Si-Kyung Lee. "Rheological Properties of Dough and Quality Characteristics of Bread Containing Whey Ferment Cultured by L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227." Korean Journal for Food Science of Animal Resources 32, no. 6 (2012): 803–9. http://dx.doi.org/10.5851/kosfa.2012.32.6.803.
Full text
25
Lee, Jeong-Hoon, and Si-Kyung Lee. "Effect of Whey Ferment Cultured by L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227 on Rheological Properties of Bread Dough." Journal of the Korean Society of Food Science and Nutrition 38, no. 6 (2009): 795–800. http://dx.doi.org/10.3746/jkfn.2009.38.6.795.
Full text
26
CHAIA, ADRIANA PEREZ, AIDA PESCE de RUIZ HOLGADO, and GUILLERMO OLIVER. "Peptide Hydrolases of Propionibacteria: Effect of pH and Temperature." Journal of Food Protection 53, no. 3 (1990): 237–40. http://dx.doi.org/10.4315/0362-028x-53.3.237.
Full textAbstract:
A comparative study of the activity of peptidases belonging to the four classic propionibacteria species cultured in milk was carried out at different values of pH and temperature. Leucine aminopeptidase and proline iminopeptidase showed greater activity in Propionibacterium freudenreichii than in the other species studied. With the single exception of Propionibacterium jensenii, the propionibacteria peptidase tested exhibited greater affinity for proline than for leucine-p-nitroanilide. Optimum temperature and pH in relation to the activity of both substrates varied according to the species under consideration.
27
Tapiovaara, L., L. Lehtoranta, T. Poussa, H. Mäkivuokko, R. Korpela, and A. Pitkäranta. "Absence of adverse events in healthy individuals using probiotics – analysis of six randomised studies by one study group." Beneficial Microbes 7, no. 2 (2016): 161–69. http://dx.doi.org/10.3920/bm2015.0096.
Full textAbstract:
Consumption of live bacteria as probiotic supplements is increasing. There is, however, a lack of information on the safety of ingested probiotics. The main objective of this study was to investigate the adverse events (AEs) of specific probiotics (Lactobacillus rhamnosus GG (LGG) alone or LGG in combination with L. rhamnosus Lc705, Propionibacterium freudenreichii JS, Bifidobacterium lactis BB12, or Bifidobacterium breve 99) studied in six of our study groups’ clinical trials, by analysing individual participant data. A secondary objective was to study AEs associated with the consumed probiotic species and mixtures in three specific categories; ‘gastrointestinal disorders’, ‘respiratory, thoracic and mediastinal disorders’ and ‘infections and infestations’. Six randomised, double-blind, placebo-controlled clinical studies by our study group were included in this AE analysis (study population n=1,909). All AE data were classified according to the National Cancer Institute’s Common Terminology Criteria for Adverse Events (CTCAE) v4.0. From the 26 CTCAE System Organ Classes, we identified AEs in 20 classes among 1,909 subjects. Probiotic ingestion did not result in statistically significant differences in AEs in different groups, when compared to placebo. A subgroup analysis of gastrointestinal, respiratory, thoracic and mediastinal disorders, infections and infestations, found no differences between the intervention groups or for different probiotic combinations (risk ratio (RR) = 0.97, 95% confidence interval (CI): 0.93-1.02, P=0.30; RR=0.99, 95% CI: 0.97-1.01, P=0.35; RR=0.99, 95% CI: 0.93-1.06, P=0.62, respectively). As a conclusion, ingestion of probiotic supplementations containing LGG alone, or LGG in combination with L. rhamnosus Lc705, P. freudenreichii JS, B. breve 99, or B. lactis BB12 did not seem to cause AEs in young and elderly subjects in this analysis.
28
Vesterlund, Satu, Matti Karp, Seppo Salminen, and Arthur C. Ouwehand. "Staphylococcus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria." Microbiology 152, no. 6 (2006): 1819–26. http://dx.doi.org/10.1099/mic.0.28522-0.
Full textAbstract:
There is increasing evidence that Staphylococcus aureus may colonize the intestinal tract, especially among hospitalized patients. As Staph. aureus has been found to be associated with certain gastrointestinal diseases, it has become important to study whether this bacterium can colonize the intestinal tract and if so, whether it is possible to prevent colonization. Adhesion is the first step in colonization; this study shows that Staph. aureus adheres to mucus from resected human intestinal tissue. Certain lactic acid bacteria (LAB), mainly commercial probiotics, were able to reduce adhesion and viability of adherent Staph. aureus. In displacement assays the amount of adherent Staph. aureus in human intestinal mucus was reduced 39–44 % by Lactobacillus rhamnosus GG, Lactococcus lactis subsp. lactis and Propionibacterium freudenreichii subsp. shermanii. Moreover, adherent Lactobacillus reuteri, Lc. lactis and P. freudenreichii reduced viability of adherent Staph. aureus by 27–36 %, depending on the strain, after 2 h incubation. This was probably due to the production of organic acids and hydrogen peroxide and possibly in the case of L. reuteri to the production of reuterin. This study shows for the first time that Staph. aureus can adhere to human intestinal mucus and adherent bacteria can be displaced and killed by certain LAB strains via in situ production of antimicrobial substances.
29
Yeom, Jiah, Seongho Ma, and Young-Hee Lim. "Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio." Microorganisms 9, no. 4 (2021): 673. http://dx.doi.org/10.3390/microorganisms9040673.
Full textAbstract:
Osteoblast differentiation is important for the development of bone and the maintenance of bone density. Propionibacterium freudenreichii is a probiotic with an anti-inflammatory property. The aim of this study was to investigate the enhancement effect of P. freudenreichii MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real-time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast mineralization. Heat-killed MJ2 (hkMJ2)-treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt-related transcription factor 2. Additionally, oral administration of live or heat-killed MJ2 to ovariectomized rats inhibited osteoporosis-induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and mineralization. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.
30
Eroshin, A. I., I. I. Idiyatov, R. M. Potekhina, Yu M. Tremasov, and A. M. Tremasova. "EVALUATION OF BIOCOMPATIBILITY OF BACTERIAL ISOLATES-COMPONENTS OF BIOCONSERVANTS." Scientific Notes Kazan Bauman State Academy of Veterinary Medicine 246, no. 2 (2021): 77–80. http://dx.doi.org/10.31588/2413-4201-1883-246-2-77-80.
Full textAbstract:
The aim of this work was to study the relationship between the isolates of Bacillus subtilis EFS, Lactobacillus plantarum, Propionibacterium freudenreichii, Lactococcus lactis, and Strepto-coccus lactis, which have the potential for the development of a bacterial preparation that improves the safety of plant-based feed during harvesting and storage. The possibility of using the tested iso-lates as part of the association was identified by the drip method, which is high quality and most suitable for primary testing. The method involved direct joint cultivation of microorganisms on the surface of a dense nutrient medium and visual assessment of signs of suppression or enhancement of the growth of one culture by another. As a result of the study, the following types of interactions between microorganisms were identified: neutrality, contact progression, antagonism, and contact regression. For inclusion in the association, isolates that showed neutrality and contact progression to each other were selected. The corresponding types of relationships were characteristic of B. sub-tilis EFS, L. plantarum, P. freudenreichii, and L. lactis isolates. Based on this, six recipes were formed that are promising for further research in order to develop a biological preparation that helps to improve the safety of plant-based feed during harvesting and storage.
31
Piwowarek, Kamil, Edyta Lipińska, Elżbieta Hać-Szymańczuk, Anna Maria Kot, Marek Kieliszek, and Sylwia Bonin. "Use of Propionibacterium freudenreichii T82 Strain for Effective Biosynthesis of Propionic Acid and Trehalose in a Medium with Apple Pomace Extract and Potato Wastewater." Molecules 26, no. 13 (2021): 3965. http://dx.doi.org/10.3390/molecules26133965.
Full textAbstract:
Propionic acid bacteria are the source of many metabolites, e.g., propionic acid and trehalose. Compared to microbiological synthesis, the production of these metabolites by petrochemical means or enzymatic conversion is more profitable. The components of microbiological media account for a large part of the costs associated with propionic fermentation, due to the high nutritional requirements of Propionibacterium. This problem can be overcome by formulating a medium based on the by-products of technological processes, which can act as nutritional sources and at the same time replace expensive laboratory preparations (e.g., peptone and yeast extract). The metabolic activity of P. freudenreichii was investigated in two different breeding environments: in a medium containing peptone, yeast extract, and biotin, and in a waste-based medium consisting of only apple pomace and potato wastewater. The highest production of propionic acid amounting to 14.54 g/L was obtained in the medium containing apple pomace and pure laboratory supplements with a yield of 0.44 g/g. Importantly, the acid production parameters in the waste medium reached almost the same level (12.71 g/L, 0.42 g/g) as the medium containing pure supplements. Acetic acid synthesis was more efficient in the waste medium; it was also characterized by a higher level of accumulated trehalose (59.8 mg/g d.s.). Thus, the obtained results show that P. freudenreichii bacteria exhibited relatively high metabolic activity in an environment with apple pomace used as a carbon source and potato wastewater used as a nitrogen source. This method of propioniate production could be cheaper and more sustainable than the chemical manner.
32
Zhuge, Xin, Long Liu, Hyun-dong Shin, et al. "Development of a Propionibacterium-Escherichia coli Shuttle Vector for Metabolic Engineering of Propionibacterium jensenii, an Efficient Producer of Propionic Acid." Applied and Environmental Microbiology 79, no. 15 (2013): 4595–602. http://dx.doi.org/10.1128/aem.00737-13.
Full textAbstract:
ABSTRACTPropionic acid (PA) is an important chemical building block and is widely applied for organic synthesis, food, feedstuff, and pharmaceuticals. To date, the strains that can efficiently produce PA have includedPropionibacterium thoenii,P. freudenreichii, andP. acidipropionici. In this report, we show thatP. jenseniiATCC 4868 is also able to produce PA in much higher yields than the previously reported strains. To further improve the production capacity, aP. jensenii-Escherichia colishuttle vector was developed for the metabolic engineering ofP. jensenii. Specifically, a 6.9-kb endogenous plasmid, pZGX01, was isolated fromP. acidipropioniciATCC 4875 and sequenced. Since the sequencing analysis indicated that pZGX01 could encode 11 proteins, the transcriptional levels of the corresponding genes were also investigated. Then, aP. jensenii-Escherichia colishuttle vector was constructed using the pZGX01 plasmid, theE. colipUC18 plasmid, and a chloramphenicol resistance gene. Interestingly, not only could the developed shuttle vector be transformed intoP. jenseniiATCC 4868 and 4870, but it also could be transformed intofreudenreichiiATCC 6207 subspecies ofP. freudenreichii. Finally, the glycerol dehydrogenase gene (gldA) fromKlebsiella pneumoniaewas expressed inP. jenseniiATCC 4868 with the constructed shuttle vector. In a 3-liter batch culture, the PA production by the engineeredP. jenseniiATCC 4868 strain reached 28.23 ± 1.0 g/liter, which was 26.07% higher than that produced by the wild-type strain (22.06 ± 1.2 g/liter). This result indicated that the constructed vector can be used a useful tool for metabolic engineering ofP. jensenii.
33
Leverrier, Pauline, Diliana Dimova, Vianney Pichereau, Yanick Auffray, Patrick Boyaval, and Gwénaël Jan. "Susceptibility and Adaptive Response to Bile Salts in Propionibacterium freudenreichii: Physiological and Proteomic Analysis." Applied and Environmental Microbiology 69, no. 7 (2003): 3809–18. http://dx.doi.org/10.1128/aem.69.7.3809-3818.2003.
Full textAbstract:
ABSTRACT Tolerance to digestive stresses is one of the main factors limiting the use of microorganisms as live probiotic agents. Susceptibility to bile salts and tolerance acquisition in the probiotic strain Propionibacterium freudenreichii SI41 were characterized. We showed that pretreatment with a moderate concentration of bile salts (0.2 g/liter) greatly increased its survival during a subsequent lethal challenge (1.0 g/liter, 60 s). Bile salts challenge led to drastic morphological changes, consistent with intracellular material leakage, for nonadapted cells but not for preexposed ones. Moreover, the physiological state of the cells during lethal treatment played an important role in the response to bile salts, as stationary-phase bacteria appeared much less sensitive than exponentially growing cells. Either thermal or detergent pretreatment conferred significantly increased protection toward bile salts challenge. In contrast, some other heterologous pretreatments (hypothermic and hyperosmotic) had no effect on tolerance to bile salts, while acid pretreatment even might have sensitized the cells. Two-dimensional electrophoresis experiments revealed that at least 24 proteins were induced during bile salts adaptation. Identification of these polypeptides suggested that the bile salts stress response involves signal sensing and transduction, a general stress response (also triggered by thermal denaturation, oxidative toxicity, and DNA damage), and an alternative sigma factor. Taken together, our results provide new insights into the tolerance of P. freudenreichii to bile salts, which must be taken into consideration for the use of probiotic strains and the improvement of technological processes.
34
Holma, Reetta, Riina A. Kekkonen, Katja Hatakka та ін. "Consumption of Galactooligosaccharides together with Probiotics Stimulates the In Vitro Peripheral Blood Mononuclear Cell Proliferation and IFNγ Production in Healthy Men". ISRN Immunology 2011 (30 листопада 2011): 1–6. http://dx.doi.org/10.5402/2011/584682.
Full textAbstract:
Probiotics and prebiotics modify the intestinal environment and could have immunomodulatory effects. The proliferation of spontaneous and phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs) and their production of interleukin-4, interleukin-5, transforming growth factor-β1, and interferon-γ (IFNγ) were determined in eighteen men at the baseline and during a 2-week period of probiotics (mixture of Lactobacillus rhamnosus GG, Lactobacillus rhamnosus LC705, Propionibacterium freudenreichii ssp. shermanii JS, and Bifidobacterium breve Bb99) and galactooligosaccharides (GOSs) (3.8 g/day). The spontaneous and stimulated proliferation of PBMC increased from the baseline during probiotics+GOS (P<0.001). The secretion of IFNγ, but not other cytokines, by stimulated PBMC increased during the same period (P<0.05). In conclusion, the consumption of this probiotic mixture including GOS appears to increase the capacity of PBMC to proliferate and release IFNγ selectively in healthy men.
35
Hervé, Christophe, Marc Fondrevez, Angélique Chéron, Frédérique Barloy-Hubler, and Gwénaël Jan. "Transcarboxylase mRNA: A marker which evidences P. freudenreichii survival and metabolic activity during its transit in the human gut." International Journal of Food Microbiology 113, no. 3 (2007): 303–14. http://dx.doi.org/10.1016/j.ijfoodmicro.2006.08.013.
Full text
36
Takahashi, Eiji, Katsuhiko Nakamichi, and Masakatsu Furui. "R-(−)-mandelic acid production from racemic mandelic acids using Pseudomonas polycolor IFO 3918 and Micrococcus freudenreichii FERM-P 13221." Journal of Fermentation and Bioengineering 80, no. 3 (1995): 247–50. http://dx.doi.org/10.1016/0922-338x(95)90824-j.
Full text
37
Treimo, Janneke, Gerd Vegarud, Thor Langsrud, and Knut Rudi. "Use of DNA Quantification To Measure Growth and Autolysis of Lactococcus and Propionibacterium spp. in Mixed Populations." Applied and Environmental Microbiology 72, no. 9 (2006): 6174–82. http://dx.doi.org/10.1128/aem.00515-06.
Full textAbstract:
ABSTRACT Autolysis is self-degradation of the bacterial cell wall that results in the release of enzymes and DNA. Autolysis of starter bacteria, such as lactococci and propionibacteria, is essential for cheese ripening, but our understanding of this important process is limited. This is mainly because the current tools for measuring autolysis cannot readily be used for analysis of bacteria in mixed populations. We have now addressed this problem by species-specific detection and quantification of free DNA released during autolysis. This was done by use of 16S rRNA gene single-nucleotide extension probes in combination with competitive PCR. We analyzed pure and mixed populations of Lactococcus lactis subsp. lactis and three different species of Propionibacterium. Results showed that L. lactis subsp. lactis INF L2 autolyzed first, followed by Propionibacterium acidipropionici ATCC 4965, Propionibacterium freudenreichii ISU P59, and then Propionibacterium jensenii INF P303. We also investigated the autolytic effect of rennet (commonly used in cheese production). We found that the effect was highly strain specific, with all the strains responding differently. Finally, autolysis of L. lactis subsp. lactis INF L2 and P. freudenreichii ISU P59 was analyzed in a liquid cheese model. Autolysis was detected later in this cheese model system than in broth media. A challenge with DNA, however, is DNA degradation. We addressed this challenge by using a DNA degradation marker. We obtained a good correlation between the degradation of the marker and the target in a model experiment. We conclude that our DNA approach will be a valuable tool for use in future analyses and for understanding autolysis in mixed bacterial populations.
38
Thierry, Anne, Marie-Bernadette Maillard, and Mireille Yvon. "Conversion of l-Leucine to Isovaleric Acid by Propionibacterium freudenreichii TL 34 and ITGP23." Applied and Environmental Microbiology 68, no. 2 (2002): 608–15. http://dx.doi.org/10.1128/aem.68.2.608-615.2002.
Full textAbstract:
ABSTRACT Several branched-chain volatile compounds are involved in the flavor of Swiss cheese. These compounds are probably produced by enzymatic conversion of branched-chain amino acids, but the flora and the pathways involved remain hypothetical. Our aim was to determine the ability of Propionibacterium freudenreichii, which is one of the main components of the secondary flora of Swiss cheese, to produce flavor compounds during leucine catabolism. Cell extracts and resting cells of two strains were incubated in the presence of l-leucine, α-ketoglutaric acid, and cofactors, and the metabolites produced were determined by high-performance liquid chromatography and gas chromatography. The first step of leucine catabolism was a transamination that produced α-ketoisocaproic acid, which was enzymatically converted to isovaleric acid. Both reactions were faster at pH 8.0 than at acidic pHs. Cell extracts catalyzed only the transamination step under our experimental conditions. Small amounts of 3-methylbutanol were also produced by resting cells, but neither 3-methylbutanal norα-hydroxyisocaproic acid was detected. l-Isoleucine and l-valine were also converted to the corresponding acids and alcohols. Isovaleric acid was produced by both strains during growth in a complex medium, even under conditions simulating Swiss cheese conditions (2.1% NaCl, pH 5.4, 24�C). Our results show that P. frendenreichii could play a significant role in the formation of isovaleric acid during ripening.
39
Romero, Danitza Xiomara, Oscar VÃctor Cárdenas AlegrÃa, VÃctor Hugo Cavero Olguin, and MarÃa Teresa Ãlvarez Aliaga. "Detection of luxS gene expression under stressing factors for biofilm formation by Propionibacterium acidipropionici and Propionibacterium freudenreichii." JOURNAL OF ADVANCES IN BIOTECHNOLOGY 5, no. 2 (2015): 604–13. http://dx.doi.org/10.24297/jbt.v5i2.1558.
Full textAbstract:
Gene expression constitutes an important role in cellular communication, setting mechanisms for biofilm formation. Genes can be used as molecular markers to monitor viability, stability and maintenance of biofilm eg. in biofilm reactors, bioremediation and biotransformation frequently under stressing conditions to enhance or limit the biofilm formation. In the present study, no pathogenic microorganisms of industrial interest were used. Propionibacterium freudenreichiisubsp. shermanii DSM 4902T and P. acidipropionici DSM 4900Tstrains cannot produce biofilm in culture conditions previously reported. In this regard, chemical culture conditions were modified to stimulate biofilm formation in both strains and determine that under stressing conditions such as 0.6 M NaCl, 1.8 M glucose and 10 gL-1 yeast extract both Propionibacterium produce biofilm. Finally, luxS expression was identified in biofilm of both strains by modified fluorescent in situ hybridization expression (FISH expression).
40
EL-NEZAMI, HANI, HANNU MYKKÄNEN, PASI KANKAANPÄÄ, SEPPO SALMINEN, and JORMA AHOKAS. "Ability of Lactobacillus and Propionibacterium Strains to Remove Aflatoxin B1 from the Chicken Duodenum." Journal of Food Protection 63, no. 4 (2000): 549–52. http://dx.doi.org/10.4315/0362-028x-63.4.549.
Full textAbstract:
The ability of Lactobacillus rhamnosus strains GG and LC-705 to remove AFB1 from the intestinal luminal liquid medium has been tested in vivo using a chicken intestinal loop technique. In this study, the GG strain of L. rhamnosus decreased AFB1 concentration by 54% in the soluble fraction of the luminal fluid within 1 min. This strain was more efficient in binding AFB1 compared with L. rhamnosus strain LC-705 (P &lt; 0.05) that removed 44% of AFB1 under similar conditions. Accumulation of AFB1 into the intestinal tissue was also determined. There was a 74% reduction in the uptake of AFB1 by the intestinal tissue, in the presence of L. rhamnosus strain GG compared with 63% and 37% in the case of Propionibacterium freudenreichii ssp. shermanii JS and L. rhamnosus strain LC-705, respectively. The complexes formed in vitro between either L. rhamnosus strain GG or L. rhamnosus strain LC-705 and AFB1 were stable under the luminal conditions for a period of 1 h.
41
Deborde, Catherine, and Patrick Boyaval. "Interactions between Pyruvate and Lactate Metabolism in Propionibacterium freudenreichii subsp.shermanii: In Vivo 13C Nuclear Magnetic Resonance Studies." Applied and Environmental Microbiology 66, no. 5 (2000): 2012–20. http://dx.doi.org/10.1128/aem.66.5.2012-2020.2000.
Full textAbstract:
ABSTRACT In vivo 13C nuclear magnetic resonance spectroscopy was used to elucidate the pathways and the regulation of pyruvate metabolism and pyruvate-lactate cometabolism noninvasively in living-cell suspensions of Propionibacterium freudenreichiisubsp. shermanii. The most important result of this work concerns the modification of fluxes of pyruvate metabolism induced by the presence of lactate. Pyruvate was temporarily converted to lactate and alanine; the flux to acetate synthesis was maintained, but the flux to propionate synthesis was increased; and the reverse flux of the first part of the Wood-Werkman cycle, up to acetate synthesis, was decreased. Pyruvate was consumed at apparent initial rates of 148 and 90 μmol · min−1 · g−1 (cell dry weight) when it was the sole substrate or cometabolized with lactate, respectively. Lactate was consumed at an apparent initial rate of 157 μmol · min−1 · g−1when it was cometabolized with pyruvate. P. shermanii used several pathways, namely, the Wood-Werkman cycle, synthesis of acetate and CO2, succinate synthesis, gluconeogenesis, the tricarboxylic acid cycle, and alanine synthesis, to manage its pyruvate pool sharply. In both types of experiments, acetate synthesis and the Wood-Werkman cycle were the metabolic pathways used most.
42
ANNOUS, BASSAM A., and MICHAEL F. KOZEMPEL. "Influence of Growth Medium on Thermal Resistance of Pediococcus sp. NRRL B-2354 (Formerly Micrococcus freudenreichii) in Liquid Foods†." Journal of Food Protection 61, no. 5 (1998): 578–81. http://dx.doi.org/10.4315/0362-028x-61.5.578.
Full textAbstract:
Pediococcus sp. is a nonpathogenic heat-resistant spoilage organism that has been used as a test organism in milk pasteurization studies. These characteristics make this bacterium an attractive test organism to study the mode of bacterial thermal inactivation in a food pilot plant. We report here the effect of growth medium on the thermal D value of this organism in skim milk, whole liquid egg, 10% glucose solution, pineapple juice, apple juice, tomato juice, and water at 60°C. Thermal inactivation was done in a submerged coil; D values were calculated from the linear portion of the survival curves by linear regression analysis. The range of D values of stationary-phase cells grown at 28°C in tryptone glucose yeast extract (TGY) or tryptic soy broth (TSB) was 0.14 to 12.05 min in all heating menstrua tested. The TSB-grown cells exhibited the highest thermal resistance with skim milk and 10% glucose solution as the heating menstrua. Survival curves of the TGY-grown cells indicated the presence of a cell population heterogeneous in thermal resistance. The TSB-grown cells exhibited a cell population uniform in thermal resistance and with a lag time for thermal inactivation. When compared to TGY-grown cells, Pediococcus sp. grown in TSB showed a significant (P &lt; 0.05) increase in D values by up to eightfold in all heating menstrua. Results from this study suggested that thermal inactivation of Pediococcus sp. was dependent on the growth medium and on the heating menstruum with respect to both pH and composition.
43
STEPHENS, T. P., G. H. LONERAGAN, E. KARUNASENA, and M. M. BRASHEARS. "Reduction of Escherichia coli O157 and Salmonella in Feces and on Hides of Feedlot Cattle Using Various Doses of a Direct-Fed Microbial." Journal of Food Protection 70, no. 10 (2007): 2386–91. http://dx.doi.org/10.4315/0362-028x-70.10.2386.
Full textAbstract:
In this study, the effectiveness of direct-fed microbials at reducing Escherichia coli O157 and Salmonella in beef cattle was evaluated. Steers (n = 240) received one of the following four treatment concentrations: control = lactose carrier only; low = 1 × 107 CFU per steer daily Lactobacillus acidophilus NP51; medium = 5 × 108 CFU per steer daily L. acidophilus NP51; and high = 1 × 109 CFU per steer daily L. acidophilus NP51. Low, medium, and high diets also included 1 × 109 CFU per steer Propionibacterium freudenreichii NP24. Feces were collected from each animal at allocation of treatment and found to have no variation (P = 0.54) between cohorts concerning E. coli O157 recovery. Feces and hide swabs were collected at harvest and analyzed for the presence of E. coli O157 by immunomagnetic separation and Salmonella by PCR. No significant dosing effects were detected for E. coli O157 recovery from feces at the medium dose or from hides at the medium and high doses. E. coli O157 was 74% (P &lt; 0.01) and 69% (P &lt; 0.01) less likely to be recovered in feces from animals receiving the high and low diets, respectively, compared with controls. Compared with controls, E. coli O157 was 74% (P = 0.05) less likely to be isolated on hides of cattle receiving the low dose. No significant dosing effects were detected for Salmonella recovery from feces at the medium and low doses or from hides at any doses. Compared with controls, Salmonella was 48% (P = 0.09) less likely to be shed in feces of cattle receiving the high dose. No obvious dose-response of L. acidophilus NP51 on recovery of E. coli O157 or Salmonella was detected in our study.
44
Herring, Ellen, Jase Ball, Elizabeth Kegley, et al. "PSXIV-35 Evaluation of a direct fed microbial in newly received, high-risk beef calves and the subsequent impact on performance and health." Journal of Animal Science 97, Supplement_3 (2019): 432–33. http://dx.doi.org/10.1093/jas/skz258.857.
Full textAbstract:
Abstract Crossbred beef calves [n = 240, body weight (BW) = 257 ± 3.5 kg] were obtained on 3 dates (block, 8 pens/block) and were assigned randomly to 1 of 2 treatments: 1) CON = top-dress supplement (0.11 kg/d) with no direct-fed microbial, 2) BOV = top dress supplement (0.11 kg/d) that provided 113.5 mg/d of a direct-fed microbial (BOVAMINE DEFEND®, 2 g/d, Lactobacillus animalis, Propionibacterium freudenreichii, 1 × 109 CFU2/g). Study objective was to determine the effect of BOV on growth performance and health when included in the diet. From d 0 to 14, ADG was improved (P = 0.05) for BOV compared to CON as ADG were 0.89 and 0.74 kg, respectively. Overall ADG over the 43-d receiving period was not different (P = 0.65); numerically there was a 0.03 kg improvement in BOV (0.90) compared to CON (0.87 kg). The percentage of calves treated for clinical BRD with the first treatment antibiotic (florfenicol) was not affected (P = 0.40); however, was numerically reduced by 6.8% for BOV (61.2% morbidity) compared to CON (68.0% morbidity). There was a trend for the percentage of calves treated with a second antibiotic (enrofloxacin) to be reduced (P = 0.17) for calves supplemented with BOV (9.1%) compared to CON (15.2%). There was also a trend for the mean total number of antibiotics used to be reduced (P = 0.16) in BOV compared to CON. Overall antibiotic cost, was not affected (P = 0.23) by BOV supplementation; however, numerically there was a $3.27 reduction in antibiotic cost for BOV compared to CON. The supplementation of BOV in high-risk calves may improve growth performance and health, reduce clinical BRD in calves after first treatment and could potentially reduce the use of antibiotics in calves at a high risk for BRD during the receiving period.
45
Leversee, Jay A., and Bonita A. Glatz. "Detection of the Bacteriocin Propionicin PLG-1 with Polyvalent Anti-PLG-1 Antiserum." Applied and Environmental Microbiology 67, no. 5 (2001): 2235–39. http://dx.doi.org/10.1128/aem.67.5.2235-2239.2001.
Full textAbstract:
ABSTRACT Polyclonal antibodies against the bacteriocin propionicin PLG-1 were produced in rabbits at high titer (256,000 to 512,000, as determined by indirect enzyme-linked immunosorbent assay [ELISA]). Anti-PLG-1 antiserum neutralized the antimicrobial activity of PLG-1 preparations in a well diffusion assay. Cross-reacting protein was detected using an indirect ELISA of the culture supernatant from a fed-batch fermentation of the producer strainPropionibacterium thoenii P127 within the first 24 h of incubation, but bacteriocin activity was not detected in the same culture until 217 h of incubation. Culture supernatants from 156 strains of classical dairy propionibacteria were tested by indirect ELISA at 5 and 12 days of incubation for production of cross-reacting protein and by well diffusion assay for bacteriocin activity. Cross-reacting protein was detected in 52 strains: all of the tested strains of P. thoenii, most of the strains ofPropionibacterium jensenii, and a minority of the Propionibacterium acidipropionici andPropionibacterium freudenreichii strains. Of these 52 strains, only 4 strains of P. thoenii showed bacteriocin activity in a well diffusion assay. Eight bacteriocin-negative mutants of strain P127 were negative in both ELISA and well diffusion assays. Western blot analysis showed that three protein bands bound anti-PLG-1 antibodies in culture supernatants: a 9.1-kDa band that is assumed to be the PLG-1 monomer and 16.2- and 27.5-kDa bands that may be precursors, multimers, or complexes of PLG-1.
46
Pyrochta, Václav, Libor Kalhotka, and Petr Doležal. "The effect silage aditives supplementation on dynamic fermentation process, quality and aerobic stability of corn silage." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 56, no. 1 (2008): 157–64. http://dx.doi.org/10.11118/actaun200856010157.
Full textAbstract:
In the experiment, the effect of additives supplementation on the fermentation quality of corn silage was examined, compared with the untreated control (K). The aditive „A“ contained bacterial component of (Propionibactrium acidipropionici – MA126/4U 3*1010 and Lactobacillus plantarum – MA18/5U). The effective substances of bacterial inoculants „B“, selected were bacterial strains of (Lactobacillus casei ssp. rhamnosus LC – 705 DSM 7061 4*1011, Propionibacterium freudenreichii spp. shermanii JS DSM 6067 2-4*1011). There were used as effective substances of bacterial inoculants „C“ lactic bacteria and enzyme (Lactobacillus plantarum CCM 3769 1.67*1010, Lactococcus lactis CCM 4754 1.67*1010, Enterococcus faecium CCM 6226 1.67*1010, Pediococcus pentosaceus CCM 3770 1,67*1010, cellulase, hemicellulase, sodium benzoate). They were applied in the dose of prescript by producer. At conservations with all aditivum were statistically significant (P < 0.01) increase of lactic acid formation from 55.31±9.72 g/kg DM of control silage to 59.60±10.84 g/kg DM aditivum „A“, 59.36±10.04 g/ kg DM aditivum „B“ rather to 60.74±9.90 g/kg DM aditivum „C“. Aditives „A“ and „B“ were statistically significant (P < 0.01) increase propoinic acid and total fermentation acid content in silages occured. The fermentation characteristics in the microbial aditivum silages by us were more favourable. The date of fermentation was statistically significant (P < 0.01) increase the contents of acetic acid from 45.49±2.83 g/kg DM of 4st day to 63.07±4.25 g/kg DM of 32ndday rather to 67.70±2.94 g/kg DM of 64st day. There were statistically significant (P < 0.01) increase contents of acetic acid and total acid content. The date of fermentation was statistically significant (P < 0.01) degressive of pH.
47
Souza, Fabíola Suano, Renata Rodrigues Cocco, Roseli Oselka S. Sarni, Márcia Carvalho Mallozi, and Dirceu Solé. "Prebióticos, probióticos e simbióticos na prevenção e tratamento das doenças alérgicas." Revista Paulista de Pediatria 28, no. 1 (2010): 86–97. http://dx.doi.org/10.1590/s0103-05822010000100014.
Full textAbstract:
OBJETIVO: Avaliar o papel dos probióticos, prebióticos e simbióticos no equilíbrio do sistema imunológico do lactente, bem como seu efeito preventivo no desenvolvimento de doenças alérgicas na criança. FONTE DE DADOS: A partir do levantamento de todos os ensaios clínicos duplo-cegos e randômicos em seres humanos, publicados nos últimos cinco anos na base de dados Medline e que contivessem unitermos relacionados a prebióticos (oligossacarídeos), probióticos e simbióticos versus hipersensibilidade, analisou-se seu papel quanto à utilização em doenças alérgicas. SÍNTESE DE DADOS: Foram incluídos nesta revisão três trabalhos com prebióticos, os quais utilizaram a mistura GOS:FOS (9:1) em fórmulas infantis em lactentes nos primeiros meses de vida; 24 trabalhos com probióticos, sendo os micro-organismos utilizados na suplementação L. rhamnosus GG, B. lactis, L. casei, L. paracasei, L. reuteri, L. acidophilus, B. longum, B. breve e P. freudenreichii sp., e dois estudos com simbióticos. CONCLUSÕES: Apesar das evidências de benefícios da suplementação precoce de probióticos com algumas cepas específicas, prebióticos e simbióticos na prevenção da dermatite atópica, em crianças de alto risco para alergias, e do uso de probióticos no tratamento das dermatites atópicas moderadas e graves mediadas por IgE, há necessidade de ampliar os estudos quanto ao tempo de observação dos indivíduos suplementados, quanto à segurança e aos efeitos em longo prazo
48
Abdul Rahman, Norafizah, Mohd Ridzwan Abd Halim, Noraniza Mahawi, Hazira Hasnudin, Jameel R. Al-Obaidi, and Norhani Abdullah. "Determination of the Use ofLactobacillus plantarumandPropionibacterium freudenreichiiApplication on Fermentation Profile and Chemical Composition of Corn Silage." BioMed Research International 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/2038062.
Full textAbstract:
Corn was inoculated withLactobacillus plantarumandPropionibacterium freudenreichiisubsp.shermaniieither independently or as a mixture at ensiling, in order to determine the effect of bacterial additives on corn silage quality. Grain corn was harvested at 32–37% of dry matter and ensiled in a 4 L laboratory silo. Forage was treated as follows: bacterial types: B0 (without bacteria-control), B1(L. plantarum), B2 (P. freudenreichiisubsp.shermanii), and B3 (combination ofL. plantarumandP. freudenreichiisubsp.shermanii). Each 2 kg of chopped forage was treated with 10 mL of bacterial culture and allowed to ferment for 27 days. The first experiment determined the most suitable wavelength for detection of bacteria (490 nm and 419 nm for B1 and B2, resp.) and the preferable inoculation size (1 × 105 cfu/g). The second experiment analysed the effect of B1 and B2 applied singly or as a mixture on the fermentation characteristics and quality of corn silage.L. plantarumalone increased crude protein (CP) and reduced pH rapidly. In a mixture withP. freudenreichii, the final pH was the lowest compared to other treatments. As a mixture, inclusion of bacteria resulted in silage with lower digestibility than control. Corn silage treated withL. plantarumorP. freudenreichiieither alone or mixed together produced desirable silage properties; however, this was not significantly better than untreated silage.
49
Vidra, Aladár, András József Tóth, and Áron Németh. "Complex whey utilization: the propionic acid alternative." Waste Treatment and Recovery 2, no. 1 (2017): 9–12. http://dx.doi.org/10.1515/lwr-2017-0002.
Full textAbstract:
Abstract Whey is the complex waste of the dairy industry. Despite the fact, that it has numerous applications (like different form of food supplements), its major amount is still handled as waste. The carbohydrate, protein and lactic acid content, as well as the COD and BOD, are sufficiently high warranting disposal as waste resulting in high costs; however, their levels are insufficient for the cost-effective isolation and purification. Most of the numerous reports on whey utilisation focus on lactose utilization, while lactic acid removal is complex, but necessary, particularly in case of sour whey decontamination. According to our best knowledge among the microbial fermentation, the only lactic acid (as carbon source) utilization process is propionic acid fermentation. Propionic acid is an attractive product with a wide application range. In this study, two propionic acid producing microorganisms were investigated in terms of industrial applicability. The propionic acid producing bacteria are generally characterized by anaerobic metabolism (except the pathogenic P. acne); but, for application in a biorefinery, facultative anaerobe behavior is the most appropriate and cost-effective. In this study, the aero-tolerances of Propionibacterium freudenreichii subsp. shermanii and Propionibacterium acidipropionici were examined; their propionic acid-producing properties (yield, concentration, substrate preference, productivity) were compared.
50
ZÁRATE, GABRIELA, ADRIANA PÉREZ CHAIA, SILVIA GONZÁLEZ та GUILLERMO OLIVER. "Viability and β-Galactosidase Activity of Dairy Propionibacteria Subjected to Digestion by Artificial Gastric and Intestinal Fluids". Journal of Food Protection 63, № 9 (2000): 1214–21. http://dx.doi.org/10.4315/0362-028x-63.9.1214.
Full textAbstract:
An important criterion to consider in the selection of strains for dietary adjuncts is the ability of the microorganisms to survive the severe conditions of acidity and bile concentrations usually found in the gastrointestinal tract. In the present work, we report the effects of digestions by artificial gastric and intestinal fluids on β-galactosidase activity and survival of four strains of dairy propionibacteria previously selected by their bile tolerance and β-galactosidase activity. The strains were exposed to artificial gastric juice at pH values between 2 and 7 and then subjected to artificial intestinal digestion. Both viability and β-galactosidase activity were seriously affected at pH 2. Skim milk and Emmental cheese juice exerted a protective effect on the parameters tested. The trypsin present in the intestinal fluid inactivated the enzyme β-galactosidase in strains of Propionibacterium freudenreichii but not in Propionibacterium acidipropionici. Moreover, the presence of bile salts enhanced the β-galactosidase activity of these strains by permeabilization of the cells during the first hour of exposure. The intestinal transit rate confirmed the permanence of the bacteria in the intestine for long enough to be permeabilized. These results suggest that P. acidipropionici would be a good source of β-galactosidase activity in the intestine. We also propose a practical and effective in vitro method as a tool of screening and selection of potential probiotic bacteria.