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1

Rachmawati, Laili, Emiliana Anggriyani, and Nur Mutia Rosiati. "Technology of Free Chrome Tanning Process: Optimal Level of Formaldehyde as Tanning Agent for Mondol Stingray (Himantura gerrardi)." Leather and Footwear Journal 20, no. 3 (2020): 277–86. http://dx.doi.org/10.24264/lfj.20.3.6.

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Chrome (VI) has a high level of toxicity. Formaldehyde is potential to be developed as a substitute of chrome tanning agent. The aims of this study are to explain the amount of formaldehyde bound, shrinkage temperature, tensile strength, and tear strength on Mondol stingray (Himantura gerrardi) leather. Pickled mondol stingray has been used as raw material. The study was divided into 4 treatments, that is P.I (formaldehyde 2%), P.II (formaldehyde 4%), P.III (formaldehyde 6%), and P.IV (formaldehyde 8%). The method used is drum tanning. Shrinkage temperatures, tensile strength and tear strength
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2

van Putten, Jos P. M., Thomas D. Duensing, and John Carlson. "Gonococcal Invasion of Epithelial Cells Driven by P.IA, a Bacterial Ion Channel with GTP Binding Properties." Journal of Experimental Medicine 188, no. 5 (1998): 941–52. http://dx.doi.org/10.1084/jem.188.5.941.

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The neisserial porin P.I is a GTP binding protein that forms a voltage-gated channel that translocates into mammalian cell membranes and modulates host cell signaling events. Here, we report that P.I confers invasion of the bacterial pathogen Neisseria gonorrhoeae into Chang epithelial cells and that this event is controlled by GTP, as well as other phosphorus-containing compounds. Bacterial invasion was observed only for strains carrying the P.IA subtype of porin, which is typically associated with the development of disseminated neisserial disease, and did not require opacity outer membrane
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3

Shmeleva, A. V., and D. V. Barteneva. "PETR IVANOVICH BARTENEV AS A FOUNDER OF PUSHKINISTIK. The 190th anniversary of the birth of PI. Bartenev." Язык и текст 5, no. 4 (2018): 32–41. http://dx.doi.org/10.17759/langt.2018050405.

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In 2019, 220 years since the birth of the great Russian national poet A.S. Pushkin and 190 years since the birth of P.I. Bartenev. The right to immortality is justified by people's memory and love. And A.S. Pushkin, and PI Bartenev belonged to the oldest noble families. P.I. Bartenev, following A.S. Pushkin, "a proud ancient man of his kind" and zealously serving the Fatherland, preserves rare documents of the era, spreading them accessible to the Russian public. Interest in the preservation of Pushkin's heritage was not accidental. As a student at Moscow University P.I. Bartenev could get car
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4

Königová, A., G. Hrčková, S. Velebný, M. Dolinská, L. Molnár, and M. Várady. "Effect of albendazole therapy on susceptible and resistant Haemonchus contortus larvae in Mongolian gerbils (Meriones unguiculatus) and distribution of inflammatory cells in the stomach wall." Helminthologia 49, no. 4 (2012): 211–20. http://dx.doi.org/10.2478/s11687-012-0040-z.

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AbstractThe effect of albendazole therapy on the reduction of drugsusceptible and drug-resistant strains of Haemonchus contortus larvae on day 10 post infection (p.i.), distribution and the relative numbers of innate immunity cells — eosinophils/neutrophils and mast cells in the stomach wall of immunosupressed Mongolian gerbils on days 4/1, 7/4, 10/7 and 14/11 post infection/post therapy (p.i./p.t.) were investigated in the present study. The efficacy of albendazole was significantly lower on benzimidazole (BZ) resistant larvae (L3 and L4 stages) (58.92 %) than the efficacy on susceptible stra
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5

Söder, Birgitta, Håkan Källmén, Tülay Yucel-Lindberg, and Jukka H. Meurman. "Periodontal microorganisms and diagnosis of malignancy: A cross-sectional study." Tumor Biology 43, no. 1 (2021): 1–9. http://dx.doi.org/10.3233/tub-200066.

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BACKGROUND: Oral infections associate statistically with cancer. OBJECTIVE: We hypothesized that certain periodontal microorganisms might specifically link to malignancies in general and set out to investigate this in our ongoing cohort study. METHODS: A sample of 99 clinically examined patients from our cohort of 1676 subjects was used to statistically investigate the associations between harboring periodontal microorganisms Aggregatibacter actinomycetemcomitans (A.a), Porphyromonas gingivalis (P.g), Prevotella intermedia (P.i), Tannerella forsythia (T.f) and Treponema denticola (T.d). We use
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6

Sommerfelt, I. E., G. Santillán, G. Mira, M. Ribicich, A. Betti, and R. De Torres. "Toxocara canis infections in a pig model: immunological, haematological and blood biochemistry responses." Journal of Helminthology 80, no. 1 (2006): 73–77. http://dx.doi.org/10.1079/joh2005324.

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AbstractThe immunological, haematological and enzymatic responses to the inoculation in pigs of 100,000 embryonated eggs of Toxocara canis were studied. Fifteen females were inoculated and three remained as controls. Haematological values were analysed from day 7 p.i. until day 126 p.i. In the inoculated group, white blood cells were raised on day 14 p.i. and eosinophil values on days 7, 14, 21, 35 and 49 p.i. showing significant differences compared with controls (P<0.05). Absolute eosinophil counts (per ml) presented two rises, the first on days 7, 14 and 21 p.i. and the second on days 35
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7

Pavlic, Verica, Dejan Peric, Ivana Stosovic Kalezic, et al. "Identification of Periopathogens in Atheromatous Plaques Obtained from Carotid and Coronary Arteries." BioMed Research International 2021 (June 17, 2021): 1–7. http://dx.doi.org/10.1155/2021/9986375.

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Increasing attention has been paid to the possible link between periodontal disease and atherosclerosis over the past decade. The aim of this study is to investigate the presence of five periopathogens: Porphyromonas gingivalis (P.g.), Aggregatibacter actinomycetemcomitans (A.a.), Tannerella forsythia (T.f.), Treponema denticola (T.d.), and Prevotella intermedia (P.i.) in atheromatous plaques obtained from the carotid and coronary arteries in patients who underwent coronary artery bypass graft surgery and carotid endarterectomy. Group I (carotid arteries) consisted of 30 patients (mean age: 54
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8

Hong, S. J., J. H. Ahn, and H. C. Woo. "Plagiorchis muris: recovery, growth and development in albino rats." Journal of Helminthology 72, no. 3 (1998): 251–56. http://dx.doi.org/10.1017/s0022149x00016527.

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AbstractMetacercariae of Plagiorchis muris, obtained from naturally infected dragonflies, Sympetrum eroticum, successfully established in 4-week-old albino rats up to 14 days post-infection (p.i.) but by day 28 p.i. the recovery rate had significantly decreased. The genital primordia in excysted metacercariae were differentiated into those of metraterm, Mehlis' gland, ovary and cirrus pouch, with the primordial testes appearing on day 1 p.i. The vitellaria and eggs in the uterus were present in flukes on days 2 and 4 p.i., respectively. Mature flukes were established in the lower part of the s
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9

Hrádková, K., and P. Horák. "Neurotropic behaviour of Trichobilharzia regenti in ducks and mice." Journal of Helminthology 76, no. 2 (2002): 137–41. http://dx.doi.org/10.1079/joh2002113.

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AbstractThe bird nasal schistosome Trichobilharzia regenti is a new agent of cercarial dermatitis. Cercariae are able to penetrate the skin of birds and mammals including man. The parasite then attacks the central nervous system. The present study has shown that schistosomula avoid penetration of blood capillaries and enter the peripheral nerves of the legs of mice and ducks as early as 1 day post-infection (p.i.) and 1.5 days p.i., respectively. These peripheral nerves are used as a route to the spinal cord. In the specific host (duck) schistosomula were found in the spinal cord from 2 days p
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10

Rodriguez Diego, J., and T. Jiménez. "Phase parasitaire d' <em>Anocentor nitens</em> (<em> Acarina : Ixodidae</em>) chez les bovins." Revue d’élevage et de médecine vétérinaire des pays tropicaux 42, no. 2 (1989): 231–32. http://dx.doi.org/10.19182/remvt.8843.

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La phase parasitaire d'Anocentor nitens (Neumann) chez les bovins a été étudiée. Le stade larvaire est présent jusqu'à 15 jours après l'infestation (post-infestation : p.i.) en même temps que les nymphes à partir du 8ème jour p.i. Des nymphes ont été observées jusqu'au 23ème jour après l'infestation et des adultes ont été vus à partir du 15ème jour p.i. Les tiques mâles se sont montrées actives à partir du 17ème jour p.i. et le détachement des femelles gorgées a commencé entre le 22ème jour et le 24ème jour p.i. Un détachement massif est apparu à partir du 28ème jour p.i. Les sites préférentie
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11

Labarque, Geoffrey G., Hans J. Nauwynck, Kristien Van Reeth, and Maurice B. Pensaert. "Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs." Microbiology 81, no. 5 (2000): 1327–34. http://dx.doi.org/10.1099/0022-1317-81-5-1327.

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Twenty-two 4- to 5-week-old gnotobiotic pigs were intranasally inoculated with 106·0 TCID50 of porcine reproductive and respiratory syndrome virus (PRRSV) (Lelystad) and euthanized at different time intervals post-inoculation (p.i.). Bronchoalveolar lavage (BAL) cell populations were characterized, together with the pattern of virus replication and appearance of antibodies in the lungs. Total BAL cell numbers increased from 140×106 at 5 days p.i. to 948×106 at 25 days p.i. and remained at high levels until the end of the experiment. The number of monocytes/macrophages, as identified by monoclo
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12

Wright, Simon. "P.I. BLUES." Police Journal: Theory, Practice and Principles 72, no. 4 (1999): 347–51. http://dx.doi.org/10.1177/0032258x9907200410.

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13

Adams, Colin. "Mangum, P.I." Mathematical Intelligencer 26, no. 4 (2004): 22–24. http://dx.doi.org/10.1007/bf02985415.

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14

Berele, Allan. "Magnum P.I." Israel Journal of Mathematics 51, no. 1-2 (1985): 13–19. http://dx.doi.org/10.1007/bf02772954.

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15

MARSCHALL, LAURENCE A. "Maggot P.I." Sciences 40, no. 4 (2000): 47–48. http://dx.doi.org/10.1002/j.2326-1951.2000.tb03509.x.

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16

Tikhomirova, E. A., I. V. Zudina, E. S. Slazhneva, et al. "Study of the correlation between the clinical manifestations of the inflammatory periodontal diseases and the microbiome of periodontal pathogenic microflora in young representatives of a mixed population of the European region." Proceedings of the National Academy of Sciences of Belarus, Medical series 18, no. 4 (2021): 433–44. http://dx.doi.org/10.29235/1814-6023-2021-18-4-433-444.

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The age of inflammatory periodontal disease (PD) manifestations has tended to decrease over the past decades. The study of the range of periodontal pathogens in young people and their influence on the PD manifestation contributes to the predictor identification for the early prevention of this pathology.The aim was to study the correlation between the range of periodontal pathogens in the dentoalveolar sulcus/periodontal pocket (DS/PC) contents and the clinical PD manifestations in young people.We examined 28 patients (23.1 ± 0.93 years) with dental biofilm-induced gingivitis (BG), 24 patients
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17

Alvarez, Rene, Kevin S. Harrod, Wun-Ju Shieh, Sherif Zaki, and Ralph A. Tripp. "Human Metapneumovirus Persists in BALB/c Mice despite the Presence of Neutralizing Antibodies." Journal of Virology 78, no. 24 (2004): 14003–11. http://dx.doi.org/10.1128/jvi.78.24.14003-14011.2004.

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ABSTRACT Human metapneumovirus (HMPV) has emerged as an important human respiratory pathogen causing upper and lower respiratory tract infections in young children and older adults. Recent epidemiological evidence indicates that HMPV may cocirculate with respiratory syncytial virus, and HMPV infection has been associated with other respiratory diseases. In this study, we show that BALB/c mice are susceptible to HMPV infection, the virus replicates in the lungs with biphasic growth kinetics in which peak titers occur at days 7 and 14 postinfection (p.i.), and infectious HMPV can be recovered fr
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18

SATO, H., K. ISHITA, A. OSANAI, M. YAGISAWA, H. KAMIYA, and M. ITO. "T cell-dependent elimination of dividingTrypanosoma grosifrom the bloodstream of Mongolian jirds." Parasitology 128, no. 3 (2004): 295–304. http://dx.doi.org/10.1017/s0031182003004463.

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Mongolian jirds,Meriones unguiculatus, are susceptible to infection withTrypanosoma grosi, which naturally parasitizesApodemusspp. The present study investigated T cell dependence of elimination ofT. grosifrom the bloodstream of jirds byin vivoT cell depletion using a monoclonal antibody (HUSM-M.g.15). In T cell-depleted jirds, elimination ofT. grosi, particularly the dividing forms, from the bloodstream was significantly delayed, occurring at around week 3 p.i. The kinetics of serum levels of IgM and IgG specific to trypanosomes in T cell-depleted and control immunocompetent jirds were differ
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19

ROEPSTORFF, A., L. ERIKSEN, H. C. SLOTVED, and P. NANSEN. "Experimental Ascaris suum infection in the pig: worm population kinetics following single inoculations with three doses of infective eggs." Parasitology 115, no. 4 (1997): 443–52. http://dx.doi.org/10.1017/s0031182097001480.

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To study population kinetics during primary Ascaris suum infections, 3 groups of 52 pigs each were inoculated with 100, 1000, or 10000 infective eggs. In all groups, the majority of larvae was found in the liver on day 3 post inoculation (p.i.) and in the lungs on day 7 p.i. Liver white spots, caused by migrating larvae, were most numerous at day 7 p.i., whereafter they gradually healed, and only low numbers of granulation-tissue type white spots and lymphonodular white spots persisted at days 21–56 p.i. Independent of dose level, 47–58% of the inoculated eggs were recovered as larvae in the s
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20

SHINTOKU, Y., H. TAKAGI, T. KADOSAKA, et al. "Strongyloides ratti: transplantation of adults recovered from the small intestine at different days after infection into the colon of naive and infection-primed Wistar rats, and the effect of antioxidant treatment on large intestinal parasitism." Parasitology 138, no. 8 (2011): 1053–60. http://dx.doi.org/10.1017/s0031182011000631.

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SUMMARYStrongyloides ratti (Nagoya strain) is unique in that a portion of adults parasitizing the small intestine withstands ‘worm expulsion’, which starts at around day 8 post-infection (p.i.) by host immunity, and establishes in the large intestine after day 19 p.i. To investigate the mechanism, adults obtained from the small intestine at day 7 or 19 p.i. were transplanted into the colon of infection-primed immune rats. Adults obtained at day 7 p.i. were rejected quickly, whereas those obtained at day 19 p.i. could establish infection. Moreover, the body length and the number of intrauterine
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21

Sharp, G. J. E., A. W. Pike, and C. J. Secombes. "Sequential development of the immune response in rainbow trout [Oncorhynchus mykiss (Walbaum, 1792)] to experimental plerocercoid infections of Diphyllobothrium dendriticum (Nitzsch, 1824)." Parasitology 104, no. 1 (1992): 169–78. http://dx.doi.org/10.1017/s0031182000060911.

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SUMMARYDevelopment of the inflammatory response of rainbow trout to experimental infections with Diphyllobothrium dendriticum plerocercoids is described using light and electron microscopy. The cellular response to plerocercoids occurred within 2 weeks post-infection (p.i.). This was followed by an increase in leucocyte numbers during weeks 3–6 p.i., with full encapsulation of plerocercoids by week 6 p.i. Neutrophils were the first leucocytes to engage the developing plerocercoid, followed by large influxes of macrophages which transformed into epithelioid cells. With longer times p.i. the acc
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22

Theodorides, Y., S. Frydas, T. Rallis, et al. "MCP-1 and MIP-2 levels during Echinococcus granulosus infections in mice." Journal of Helminthology 75, no. 2 (2001): 205–8. http://dx.doi.org/10.1079/joh200139.

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AbstractTen BALB/c mice were infected with the cestode Echinococcus granulosus. After the infection, serum was collected at different periods of time and monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) were determined. The level of MCP-1 increased from 290±12 pg ml-1 (mean±SD) at day 20 post infection (p.i.), to a maximum of 820±23 pg ml-1 on day 60 p.i., then decreased to 460±12.6 pg ml-1 on day 130 p.i. A second peak was observed at day 150 p.i. In addition, MIP-2 was detectable in serum as late as day 100 p.i. The highest level (100±11 pg ml-1) was obser
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23

Dvorožňáková, E., Z. Hurníková, and M. Kołodziej-Sobocińska. "Kinetics of specific humoral immune response of mice infected with low doses of Trichinella spiralis, T. britovi, and T. pseudospiralis larvae." Helminthologia 47, no. 3 (2010): 152–57. http://dx.doi.org/10.2478/s11687-010-0023-x.

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Abstract The specific humoral immune response of the host (mouse) to the infection with low doses of larvae of encapsulating (Trichinella spiralis, T. britovi) and non-encapsulating (T. pseudospiralis) species was studied. Mice were experimentally infected with 10 muscle larvae of the parasite to simulate natural conditions of the infection in rodents, important reservoirs of trichinellosis. The low infective dose of T. spiralis and T. britovi did not evoke an increased specific IgM response, which is typical for the acute infection. Only T. pseudospiralis induced a higher specific IgM level i
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24

Díaz, I., L. Darwich, G. Pappaterra, J. Pujols, and E. Mateu. "Immune responses of pigs after experimental infection with a European strain of Porcine reproductive and respiratory syndrome virus." Journal of General Virology 86, no. 7 (2005): 1943–51. http://dx.doi.org/10.1099/vir.0.80959-0.

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The purpose of this experiment was to study the immune response of pigs during an experimental infection with a European strain of Porcine reproductive and respiratory syndrome virus (PRRSV). Five pigs were challenged intranasally with PRRSV strain VP21 and another five were kept as controls. Clinical course and humoral and cell-mediated responses were monitored for 70 days post-infection (p.i.). Infected pigs developed mild signs at 24 h p.i. Viraemia was detectable by nested RT-PCR until day 14 p.i. Earliest seroconversions (ELISA) were seen by day 7 p.i. (three of five animals) and, by day
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25

ANDRUSHCHENKO, N. "FACTORS THE FORMATION OF NATIONAL SELF-CONSCIOUSNESS AND EDUCATION IN BELARUS (second half of XIX – beginning of XX century)." Herald of Polotsk State University. Series E. Pedagogical sciences, no. 1 (June 3, 2024): 2–5. http://dx.doi.org/10.52928/2070-1640-2024-41-1-2-5.

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The article defines the concept of «national self-consciousness». The works of famous teachers, ethnographers, folklorists Y.F. Krachkovski, N.Y. Nikiforovski, I.I. Nosovich, E.R. Romanov, P.V. Shein, P.I. Shpilevsky, I.A. Serbov, A.K. Serzhputovski, who researched and collected the oral folk art of Belarusians (proverbs, sayings, rites, songs, traditions) are presented. Examples of ritual songs are given, which were reflected in P.M. Shpilevsky's book «Belarus in Rites and Fairy Tales». The factors of formation of national consciousness and enlightenment in Belarus in the second half of the X
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26

Graef, Josefine, Carolin Senger, Christoph Wetz, et al. "Shortened Tracer Uptake Time in GA-68-DOTATOC-PET of Meningiomas Does Not Impair Diagnostic Accuracy and PET Volume Definition." Diagnostics 10, no. 12 (2020): 1084. http://dx.doi.org/10.3390/diagnostics10121084.

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Ga-68-DOTATOC-PET/MRI can affect the planning target volume (PTV) definition of meningiomas before radiosurgery. A shorter tracer uptake time before image acquisition could allow the examination of more patients. The aim of this study was to investigate if shortening uptake time is possible without compromising diagnostic accuracy and PET volume. Fifteen patients (f = 12; mean age 52 years (34–80 years)) with meningiomas were prospectively examined with dynamic [68Ga]Ga-68-labeled [DOTA0-Phe1-Tyr3] octreotide (Ga-68-DOTATOC)-PET/MRI over 70 min before radiosurgery planning. Meningiomas were de
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27

Miranda-Saksena, Monica, Patricia Armati, Ross A. Boadle, David J. Holland, and Anthony L. Cunningham. "Anterograde Transport of Herpes Simplex Virus Type 1 in Cultured, Dissociated Human and Rat Dorsal Root Ganglion Neurons." Journal of Virology 74, no. 4 (2000): 1827–39. http://dx.doi.org/10.1128/jvi.74.4.1827-1839.2000.

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ABSTRACT The mechanism of anterograde transport of herpes simplex virus was studied in cultured dissociated human and rat dorsal root ganglion neurons. The neurons were infected with HSV-1 to examine the distribution of capsid (VP5), tegument (VP16), and glycoproteins (gC and gB) at 2, 6, 10, 13, 17, and 24 h postinfection (p.i.) with or without nocodazole (a microtubule depolymerizer) or brefeldin A (a Golgi inhibitor). Retrogradely transported VP5 was detected in the cytoplasm of the cell body up to the nuclear membrane at 2 h p.i. It was first detected de novo in the nucleus and cytoplasm a
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28

MATSUMOTO, J., K. YAGI, N. NONAKA, Y. OKU, and M. KAMIYA. "Time-course of antibody response in mice against oral infection with eggs of Echinococcus multilocularis." Parasitology 116, no. 5 (1998): 463–69. http://dx.doi.org/10.1017/s0031182098002443.

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The kinetics of serum antibody response against infection with Echinococcus multilocularis eggs was evaluated in AKR mice. The animals were infected by oral inoculation with 300 parasite eggs, and necropsied at 1, 2, 4, 6, 9, 12 and 16 weeks post-infection (p.i.), respectively. The parasite formed the laminated layer at 4 weeks p.i., the brood capsule with a massive proliferation of germinal cells at 9 weeks p.i. and protoscoleces at 16 weeks p.i. Serum antibody responses of the mice to antigen preparations from metacestodes of different stages and protoscoleces were evaluated by ELISA, immuno
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29

Fais, Theofanis, Nektarios Giadinis, Elias Papadopoulos, et al. "Effect of Toxoplasma gondii on Ram Sperm Quality after Experimental Infection." Pathogens 9, no. 12 (2020): 1004. http://dx.doi.org/10.3390/pathogens9121004.

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The aim of this study was to investigate the effect of experimental Toxoplasma gondii infection on ram sperm quality. Five months old, pre-pubertal, rams were divided into four groups (n = 8 per group). Group A was the control group; the remaining animals received per os (p.o.) 5000 oocysts per ram. Group B did not receive treatment post-infection (p.i.). Group C received sulphadimidine (intermuscular injection (i.m.) 33 mg/kg for eight days; every 48 h) two months p.i. and Group D received the same drug twice (24 h p.i. and two months later). Blood samples were collected every 15 days to dete
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30

Robertson, Lucy J. "Fluctuations in rat liver alanine-amino-transferase activity during experimental nippostrongylosis." Parasitology 98, no. 2 (1989): 301–6. http://dx.doi.org/10.1017/s0031182000062223.

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SUMMARYThe activity of the gluconeogenic enzyme, alanine-amino-transferase (ALT), in a preparation from the liver of rats was studied by means of an in vitro assay throughout the course of a primary infection of Nippostrongylus brasiliensis, established by a subcutaneous injection of approximately 4000 3rd-stage larvae. The activity was measured on days 1–14 p.i. in both uninfected and infected rats and a marked pattern in the enzyme's activity was observed. In infected rats, the activity increased from 1·46±0·19 U/g liver on day 1 p.i. to a peak on day 4 p.i. of 10·75±1·62 U/g liver, then dec
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31

ALWAN, M. J. "EXPERIMENTAL STUDY OF NOCARDIOSIS IN GUINEA PIGS." Iraqi Journal of Veterinary Medicine 24, no. 2 (2000): 251–68. http://dx.doi.org/10.30539/ijvm.v24i2.1180.

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The pathology of local isolated strain of Nocardia asteroides was study in pigs . 60 animals were divided into two groups. The 1st group (40 animals) was given a single intrapulmonary of 3.3X10'CFU of N. astoroides. The 2 nd group (20 animals) served as control . Eight animals died during the first 3 weeks post – infection while the remaining were sacrificed at 6, 12, 24, 48 hrs and 4, 8, 16, 24 and 62 days P.I. Early pathological examination revealed acute suppurative changes which seen turned into disseminated chronic form. Pyogranul matous and epithelioid granuloma have represented the main
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32

Okano, Keiju, Victor S. Mikhailov, and Susumu Maeda. "Colocalization of Baculovirus IE-1 and Two DNA-Binding Proteins, DBP and LEF-3, to Viral Replication Factories." Journal of Virology 73, no. 1 (1999): 110–19. http://dx.doi.org/10.1128/jvi.73.1.110-119.1999.

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ABSTRACT We have recently identified a DNA-binding protein (DBP) from the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) which can destabilize double-stranded DNA (V. S. Mikhailov, A. L. Mikhailova, M. Iwanaga, S. Gomi, and S. Maeda, J. Virol. 72:3107–3116, 1998). DBP was found to be an early gene product that was not present in budded or occlusion-derived virions. In order to characterize the localization of DBP during viral replication, BmNPV-infected BmN cells were examined by immunostaining and confocal microscopy with DBP antibodies. DBP first appeared as diffuse nuclear staining at
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33

MacKinnon, Barbara M., and D. L. Lee. "Age-related changes in Heligmosomoides polygyrus (Nematoda): neutral lipid content in developing oocytes." Canadian Journal of Zoology 66, no. 12 (1988): 2791–96. http://dx.doi.org/10.1139/z88-407.

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Changes in neutral lipid content of developing female gametes in Heligmosomoides polygyrus at 8, 12, 20, 40, 80, and 140 days postinfection (p.i.) were investigated and correlated with egg production by the worms over the same period. Egg production increased to day 20 p.i. when the average egg output for one female reached approximately 700 eggs/day. A decline in egg production occurred from 80 days p.i. until the end of the experiment (140 days p.i.). Neutral lipid content was low in oogonia from worms of all ages. Developing oocytes contained the highest levels of neutral lipid. There was a
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34

NARGIS, M., M. M. CHISTY, Y. IHAMA, H. SATO, T. INABA, and H. KAMIYA. "Kinetics of Trypanosoma cruzi infection in guinea-pigs, with special reference to the involvement of epidermal Langerhans' cells in the induction of immunity." Parasitology 123, no. 4 (2001): 373–80. http://dx.doi.org/10.1017/s0031182001008551.

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Several studies have confirmed that epidermal Langerhans' cells (LC) play a central role in the induction of skin-related immunological events. In order to assess the role of LC in Chagas' disease, guinea-pigs were infected intradermally with Trypanosoma cruzi, sacrificed at different time-points, and their tissues were processed for routine histology, electron microscopy and immunohistochemistry. Parasitaemia was observed earliest at day 6 p.i. with 2 peaks at days 9 and 28, and disappeared on day 56 p.i. Parasite-specific serum IgG and IgM were first detected on day 12 p.i. The level of IgG
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35

Stommel, John R., and Kathleen G. Haynes. "Inheritance of Resistance to Anthracnose Caused by Colletotrichum coccodes in Tomato (Lycopersicon esculentum)." HortScience 32, no. 3 (1997): 449C—449. http://dx.doi.org/10.21273/hortsci.32.3.449c.

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Anthracnose, caused by Colletotrichum coccodes, is a serious ripe tomato fruit rot disease. Genetic resistance to anthracnose is not available in commercial tomato cultivars, but has been reported in small-fruited Plant Introductions (P.I.), and with lesser intensity in a number of breeding lines. Transfer of high levels of resistance from these breeding lines or P.I.s to elite materials has proven difficult. Inheritance of resistance has been described as complex with at least six loci influencing resistance reactions. Segregating populations originating from a cross between a susceptible tom
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36

BARBOSA, CLEBER F., ALEXANDRE A. TONIN, ALEKSANDRO S. DA SILVA, et al. "Diphenyl diselenide and sodium selenite associated with chemotherapy in experimental toxoplasmosis: influence on oxidant/antioxidant biomarkers and cytokine modulation." Parasitology 141, no. 13 (2014): 1761–68. http://dx.doi.org/10.1017/s0031182014001073.

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SUMMARYThe aim of this study was to assess the effect of sulfamethoxazole/trimethoprim (ST) supplemented with diphenyl diselenide and sodium selenite in experimental toxoplasmosis, on oxidant/antioxidant biomarkers and cytokine levels. Eighty-four BALB/c mice were divided in seven groups: group A (negative control), and groups B to G (infected). Blood and liver samples were collected on days 4 and 20 post infection (p.i.). Levels of thiobarbituric acid (TBA) reactive substances and advanced oxidation protein products (AOPP) were assessed in liver samples. Both biomarkers were significantly inc
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37

Ogunleye, A. O., A. T. P. Ajuwape, and A. I. Adetosoye. "Modifications hématologiques chez des poulettes infectées par Salmonella Paratyphi A." Revue d’élevage et de médecine vétérinaire des pays tropicaux 62, no. 1 (2009): 23. http://dx.doi.org/10.19182/remvt.10090.

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Une analyse hématologique a été effectuée sur 25 poulettes infectées expérimentalement par voie orale avec 0,5 mL de 1 x 107 CFU/mL de Salmonella Paratyphi A. Quatre jours après l’infection (p.i.), les animaux ont présenté de l’apathie, un plumage ébouriffé, de la somnolence, des diarrhées vert jaunâtre, une baisse de la prise d’eau et d’aliments. Les paramètres hématologiques des volailles ont été enregistrés aux jours 7, 14 et 21 p.i. Les valeurs ont été analysées avec le test de comparaisons multiples de Duncan, avec P &lt; 0,05. L’hématocrite a été significativement plus élevé au jour 7 qu
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38

Audebert, F., H. Hoste, and M. C. Durette-Desset. "Life cycle ofTrichostrongylus retortaeformisin its natural host, the rabbit (Oryctolagus cuniculus)." Journal of Helminthology 76, no. 3 (2002): 189–92. http://dx.doi.org/10.1079/joh2002126.

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AbstractThe chronology of the life cycle ofTrichostrongylus retortaeformis(Zeder, 1800) (Nematoda, Trichostrongyloidea) is studied in its natural hostOryctolagus cuniculus. The free living period lasted 5 days at 24°C. Worm-free rabbits were each infectedper oswithT. retortaeformislarvae. Rabbits were killed at 12 h post-infection (p.i.) and every day from one day to 13 days p.i. By 12 h p.i., all the larvae were exsheathed and in the small intestine. The third moult occurred between 3 and 5 days p.i. and the last moult between 4 and 7 days p.i. The prepatent period lasted 12 to 13 days. The p
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39

NUÑEZ, G. G., S. N. COSTANTINO, and S. M. VENTURIELLO. "Immunoparasitological parameters of the intestinal phase of trichinellosis in rats." Parasitology 126, no. 4 (2003): 321–25. http://dx.doi.org/10.1017/s0031182003003007.

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Enzyme-linked immunosorbent assays (ELISAs) were developed in order to detect coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes (FIC) in rats experimentally infected with Trichinella spiralis. The usefulness of these assays was compared to that of a conventional ELISA for detection of serum antibodies (Abs) to muscle larvae excretory–secretory products (ML–ESP). The ELISA for CAgs was the first parameter to give a positive result but the detection was limited only to day 2 p.i. CAbs against ML–ESP and adult worm excretory–secretory products (AW–ESP) was first positive on
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40

Kirk, R. S., and J. W. Lewis. "Histopathology of Sanguinicola inermis infection in carp, Cyprinus carpio." Journal of Helminthology 72, no. 1 (1998): 33–38. http://dx.doi.org/10.1017/s0022149x00000948.

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AbstractThe histopathological response of carp to Sanguinicola inermis was investigated by serial sectioning laboratory infected fish up to 90 days post infection (d p.i.). Juvenile flukes and adults caused mechanical damage to tissues during invasion and migration up to 28 d p.i. Adults partially occluded blood vessels and may have reduced blood circulation. In the initial phase of egg production (28–42 d p.i.), eggs and emigrating miracidia in gill tissue caused breakdown of vascular integrity, necrosis, hyperplasia, haemorrhage and eosinophilic infiltration of epithelial tissue. After 42 d
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41

Henzel, Andréia, Diego G. Diel, Sandra Arenhart, Fernanda S. Flores Vogel, Rudi Weiblen, and Eduardo F. Flores. "Aspectos virológicos e clínico-patológicos da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras infectadas experimentalmente." Pesquisa Veterinária Brasileira 28, no. 3 (2008): 140–48. http://dx.doi.org/10.1590/s0100-736x2008000300003.

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A infecção genital de vacas pelo herpesvírus bovino tipo 1.2 (BoHV-1.2) pode resultar em vulvovaginite e infertilidade temporária. Após a infecção aguda, o BoHV-1 estabelece infecção latente, que pode cursar com episódios periódicos de reativação. O presente trabalho descreve os aspectos virológicos e clínico-patológicos da vulvovaginite aguda e infecção latente resultantes da inoculação de bezerras com uma amostra de BoHV-1.2 isolada de casos de balanopostite em touros. A inoculação do vírus em quatro bezerras pela via genital (10(8.1)TCID50/animal) resultou em replicação viral na mucosa geni
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42

Kirk, R. S., and J. W. Lewis. "Migration and development of the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae) in carp, Cyprinus carpio L." Parasitology 113, no. 3 (1996): 279–85. http://dx.doi.org/10.1017/s0031182000082044.

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SUMMARYThe migration and development of Sanguinicola inermis in carp fingerlings were investigated by quantitative analysis of serial sections of fish and examination of flukes from hosts up to 90 days p.i. Cercariae penetrated through the epidermis and migrated through connective tissue and muscle to establish in the blood system and commence egg production within 28 days p.i. Most flukes established in the ventral blood system and released eggs which accumulated in gill epithelial tissue. A minority of flukes inhabited dorsal blood vessels and shed eggs which lodged in connective tissue and
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43

Kotnik, T., and M. Černe. "Clinical and Histopathological Evaluation of Terbinafine Treatment in Cats Experimentally Infected with Microsporum canis." Acta Veterinaria Brno 75, no. 4 (2006): 541–47. http://dx.doi.org/10.2754/avb200675040541.

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The efficacy of terbinafine hydrochloride (Lamisil©, Novartis) in the treatment of 27 M. canis infected cats was followed. Treatment was started on the 17th day post inoculation (p.i.), when successful experimental infection was proved. Nine cats were treated with low-dose terbinafine 10-20 mg/kg QD (LD group), nine cats were treated with high-dose terbinafine 30-40 mg/kg QD (HD group) and nine were left untreated as a control group (C group). The efficacy of the treatment was evaluated using Wood's lamp examination, fungal culture and histopathology. All cats were positive 14 days p.i. Sixty
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44

von Knoblauch, Tammo, Annette B. Jensen, Christoph K. W. Mülling, Anton Heusinger, Heike Aupperle-Lellbach, and Elke Genersch. "Stonebrood Disease—Histomorphological Changes in Honey Bee Larvae (Apis mellifera) Experimentally Infected with Aspergillus flavus." Veterinary Sciences 12, no. 2 (2025): 124. https://doi.org/10.3390/vetsci12020124.

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Stonebrood (Aspergillus sp.) is a rare, poorly described disease of the Western honey bee (Apis mellifera) that can affect adult bees and brood. This study describes the pathogenesis using artificially reared pathogen-free Apis mellifera larvae, experimentally infected (5 × 102 spores/larva) with Aspergillus flavus. Between days 1 and 5 p.i. (larval age 4 until 8 days), five uninfected control larvae, up to five infected living larvae, and up to five infected dead larvae were examined macroscopically. Subsequently, the larvae were photographed, fixed (4% formaldehyde), and processed for histol
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45

Nagano, I., Z. Wu, T. Nakada, T. Boonmars, and Y. Takahashi. "Molecular cloning and characterization of a novel protein of Trichinella pseudospiralis excretory–secretory products." Journal of Helminthology 76, no. 2 (2002): 165–70. http://dx.doi.org/10.1079/joh2001107.

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AbstractA novel excretory–secretory (ES) protein of Trichinella pseudospiralis was produced. A cDNA library was constructed from mRNA of muscle larvae at 30 days post infection (p.i.) and immunoscreened with the antibody against ES products. A clone, designated Tp22-3, contained a cDNA transcript of 815 bp in length with a single open reading frame which encoded 244-amino acids (28407 Da in the estimated molecular mass). A database search revealed that no sequences had a homology to this predicted protein. The recombinant protein was produced in an Escherichia coli expression system. Stage spe
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46

BAHRNDORFF, S., A. B. GARCIA, H. VIGRE, et al. "Intestinal colonization of broiler chickens byCampylobacterspp. in an experimental infection study." Epidemiology and Infection 143, no. 11 (2014): 2381–89. http://dx.doi.org/10.1017/s0950268814003239.

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SUMMARYConsumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data onCampylobacterspp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens byCampylobacter jejuniover time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large di
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47

MUÑOZ, P., A. SITJÀ-BOBADILLA, and P. ÁLVAREZ-PELLITERO. "Cellular and humoral immune response of European sea bass (Dicentrarchus labrax L.) (Teleostei: Serranidae) immunized with Sphaerospora dicentrarchi (Myxosporea: Bivalvulida)." Parasitology 120, no. 5 (2000): 465–77. http://dx.doi.org/10.1017/s0031182099005855.

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The immune response of European sea bass after intracaelomic immunization with Sphaerospora dicentrarchi was studied. Fish were injected with S. dicentrarchi spores (DIC), with spores plus adjuvant (DIC+FCA), with adjuvant alone (FCA) or with PBS. Several parameters of the immune response were measured. Serum lysozyme increased significantly in DIC fish 1 week after immunization (p.i.) and it remained significantly higher in DIC+FCA fish 4 weeks p.i., and in DIC fish 8 weeks p.i. than in PBS-injected fish. The number of nitroblue tetrazolium-positive blood cells was significantly higher in DIC
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48

Brozos, C., G. Tsousis, E. Kiossis, I. Tsakmakidis, I. Karagiannis, and C. Boscos. "Effect of PRID administration post-insemination on the establishment of pregnancy of dairy cows under commercial farm conditions." Journal of the Hellenic Veterinary Medical Society 69, no. 4 (2019): 1235. http://dx.doi.org/10.12681/jhvms.16492.

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The aim of this study was to test the efficiency of progesterone supplementation post insemination (p.i.) in the fertility of dairy cows under commercial farm conditions. At initiation of the study, 405 cows from three farms -irrespective of their open days- were bred after showing heat or after synchronization. Subsequently, the cows were randomly allocated to receive a progesterone-releasing intra-vaginal device (PRID®) between D5 and D17 p.i (P4+, n=213), or to remain untreated (P4-, n=192). Pregnancy per artificial insemination (P/AI) did not differ between P4+ (42.3%) and P4- (41.2%, P =
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49

Husáková, Eva, Viera Spišáková, Róbert Herich, et al. "Expression of cytokines in chicken peripheral mononuclear blood cells (PMBCs) exposed to probiotic strains and Salmonella Enteritidis." Acta Veterinaria Brno 84, no. 1 (2015): 29–35. http://dx.doi.org/10.2754/avb201585010029.

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The mRNA expression of interleukin (IL)-1β, LITAF, iNOS, macrophage inflammatory protein (MIP1-ß), and K60 were examined in peripheral blood mononuclear cells (PMBCs). The PMBCs were isolated from the chicken blood and in vitro exposed to the probiotic strains E. faecium AL41, E. faecium H31, L. fermentum AD1, and infected with Salmonella enterica serovar Enteritidis (SE147). The PMBCs were evaluated for mRNA expression levels at 24 h and 48 h post infection (p.i.) using the reverse transcriptase polymerase chain reaction (RT-PCR). The level of expression of IL-1ß and MIP1-ß was upregulated (P
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50

Duffy, Simon P., Elisa M. Becker, Beatrixe H. Whittome, Christopher J. Lucarotti, and David B. Levin. "In vivo replication kinetics and transcription patterns of the nucleopolyhedrovirus (NeabNPV) of the balsam fir sawfly, Neodiprion abietis." Journal of General Virology 88, no. 7 (2007): 1945–51. http://dx.doi.org/10.1099/vir.0.82763-0.

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DNA replication and transcription of NeabNPV, the nucleopolyhedrovirus (NPV) of the balsam fir sawfly, Neodiprion abietis (Hymenoptera: Diprionidae), in host larvae were investigated. NPV DNA replication kinetics and gene-expression patterns have been resolved only in lepidopteran cell-culture systems and in limited in vivo experiments with lepidopteran larvae. Furthermore, there are significant differences in pathologies caused by lepidopteran NPVs, which replicate in many tissues, and hymenopteran NPVs, known to replicate in midgut epithelium only. Despite the differences in host specificity
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