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Academic literature on the topic 'P. spinosa (blackthorn)'
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Journal articles on the topic "P. spinosa (blackthorn)"
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Murati, Teuta, Marina Miletić, Josipa Kolarić, et al. "Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells." Archives of Industrial Hygiene and Toxicology 70, no. 4 (2019): 303–9. http://dx.doi.org/10.2478/aiht-2019-70-3322.
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AbstractPrunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10-200 µg/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24, 48, and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 µg/mL (p<0.001–0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma.
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Tynkevich, Y. O., L. V. Kozub, and R. A. Volkov. "Organization and polymorphysm of 5S rDNA intergenic spacer of blackthorn (Prunus spinosa L.)." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 19, no. 1-2 (2021): 40–46. http://dx.doi.org/10.7124/visnyk.utgis.19.1-2.1439.
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Aim. The 5S rDNA repeated units consist of conserved regions encoding 5S rRNA and variable intergenic spacers (IGS). The IGS sequences are commonly used as molecular markers for low-ranking phylogenetic, phylogeographical and microevolutionary studies. However, this genomic region still remains undescribed for the waist majority of genera in the Rosaceae family. Here we present the first report of the IGS molecular organization and polymorphism for the widespread member of the Rosaceae family, Prunus spinosa. Methods. PCR amplification, cloning and sequencing of 5S rDNA, bioinformatics analysis. Results. The 5SrDNA IGS of P. spinosa was cloned sequenced and compared with the IGS of three other representatives of the genus Prunus. It was found that the IGS variants present in the genome of P. spinosa differ by the number of subrepeats located at the 5’end. Each of these subrepeats contain RNA Pol III transcription termination signal. The IGS sequences of Prunus species evolved mainly by accumulation of nucleotide substitutions. The level of intragenomic similarity of P. spinosa IGS is 96.5-100%, while the similarity between the IGS of different Prunus species ranges from 73.0 to 87.3%. Conclusions. The IGS of 5S rDNA represents a useful marker for phylogenetic and taxonomic studies in the genus Prunus.Keywords: 5S rDNA, molecular evolution, Prunus spinosa.
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Katanić Stanković, Jelena S., Nenad Mićanović, Nadja Grozdanić, et al. "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia." Horticulturae 8, no. 11 (2022): 1053. http://dx.doi.org/10.3390/horticulturae8111053.
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Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.
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Katanić, Stanković Jelena S., Nenad Mićanović, Nađa Grozdanić, et al. "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia." Horticulturae 8, no. 11 (2022): 1053. https://doi.org/10.3390/horticulturae8111053.
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Abstract Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH<sup>·</sup> and ABTS<sup>·+</sup> scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC<sub>50</sub> values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.
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Miranda, Carlos, Luis G. Santesteban, and José B. Royo. "Variability in the Relationship between Frost Temperature and Injury Level for Some Cultivated Prunus Species." HortScience 40, no. 2 (2005): 357–61. http://dx.doi.org/10.21273/hortsci.40.2.357.
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The influence of the species in spring frost sensibility was determined for the Prunus species peach (P. persica (L.) Batsch), sweet cherry (P. avium L.), almond (P. dulcis (Mill.) Webb/P. amygdalus Batsch), japanese plum (P. salicina Lindl.), and blackthorn (P. spinosa L.). The confidence intervals for lethal temperatures of 10% (LT10) and 90% (LT90) bud injury were also determined. In 2000 and 2001, seven frost treatments were made for each one of the phenological stages comprised between B (first swell) and I (jacket split) in two cultivars per each species. The relationships between frost temperature and the proportion of frost damaged buds for each cultivar, year, and phenological stage were adjusted to linear regression models. The 95% confidence intervals were also calculated. The spring frost hardiness order of the species, from the least to most hardy, was as follows: sweet cherry, almond, peach, japanese plum, and blackthorn. Despite the highly homogeneous nature of the frost and bud characteristics, the temperature range for a given injury degree was quite broad, since the confidence interval's breadth for LT10 was as high as about 3 °C and as high as about 6 °C for LT90. Consequently, when critical temperatures are used in making decisions as to when to begin active frost protection, a prudent measure would be to take the temperature references from the upper limits in the confidence intervals.
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Kırca, Levent, and Kerem Mertoğlu. "Morphological and Chemical Characteristics of Prunus spinosa L. Genotypes from Denizli (Çivril)." Türk Tarım ve Doğa Bilimleri Dergisi 12, no. 2 (2025): 379–89. https://doi.org/10.30910/turkjans.1608381.
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This study was conducted to characterize the physico-chemical properties of naturally occurring blackthorn (Prunus spinosa L.) genotypes in Çivril, Denizli, Türkiye. In this context, 13 different blackthorn genotypes originating from seeds were examined in terms of color parameters, physical, and chemical properties. Physical characteristics of the fruits revealed that fruit width ranged from 7.95 mm to 14.12 mm, fruit length from 10.49 mm to 14.63 mm, and fruit weight varied between 1.82 g and 2.71 g. Notably, genotypes G3 and G5 stood out with fruit weights of 2.71 g and 2.58 g, respectively. Regarding the fruit flesh-to-seed ratio, genotype G3 exhibited the highest value of 7.10. In terms of color parameters, the L* value of the fruit skin ranged from 16.75 to 20.59, while the L* value of the fruit flesh varied between 17.48 and 20.45. Biochemical characteristics showed that the soluble solids content ranged widely from 12.00% to 23.40%, while pH values remained stable between 3.60 and 3.90. PCA analysis revealed that the first two components explained 67.13% of the total variation in the examined traits. Correlation analysis found a strong positive relation between fruit flesh color L* and b* values (r=0.95), as well as between fruit length and shape index (r=0.89). Based on the biplot results, it can be inferred that the studied P. spinosa genotypes have the potential to be evaluated in different ways. In conclusion, it can be stated that this species has high adaptability to local ecosystems and that, in the short term, more efficient genotypes can be obtained through selection, and in the long term, through hybridization.
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Athanasiadis, Vassilis, Theodoros Chatzimitakos, Konstantina Kotsou, Dimitrios Kalompatsios, Eleni Bozinou, and Stavros I. Lalas. "Utilization of Blackthorn Plums (Prunus spinosa) and Sweet Cherry (Prunus avium) Kernel Oil: Assessment of Chemical Composition, Antioxidant Activity, and Oxidative Stability." Biomass 4, no. 1 (2024): 49–64. http://dx.doi.org/10.3390/biomass4010003.
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Prunus avium L. and Prunus spinosa L. are valuable fruit-bearing trees known for their bioactive compounds and medicinal properties. However, limited research exists regarding their kernel oils. This study aimed to compare the chemical composition, quality parameters, and bioactive potential of the kernel oils extracted from Prunus avium L. and Prunus spinosa L. The kernel oils’ fatty acid and tocopherol profiles were characterized, and the presence of bioactive compounds were identified and quantified. Total polyphenol content (TPC) and antioxidant activity (AAC) were also measured, indicating the presence of bioactive compounds in both oils. Additionally, the main quality parameters, including oxidative status, were evaluated. The fatty acid analysis revealed a higher proportion of polyunsaturated fatty acids compared to monounsaturated fatty acids in both kernel oil samples. Linoleic acid (57–64%) and oleic acid (18–29%) were the major fatty acids in both Prunus avium L. and Prunus spinosa L. kernel oils. α-Eleostearic acid (11.87%) was quantified only in Prunus avium kernel oil. Furthermore, the α-, β-, γ-, and δ-tocopherol content were determined, and it was found that both kernel oils contained γ-tocopherol as the major tocopherol (~204–237 mg/Kg). TPC in Prunus avium L. kernel oil was measured at 9.5 mg gallic acid equivalents (GAE)/Kg and recorded as ~316% higher TPC than Prunus spinosa L. kernel oil. However, the recorded AAC were 11.87 and 14.22 μmol Trolox equivalent (TE)/Kg oil, respectively. Both oils recorded low peroxide values (~1.50 mmol H2O2/Kg), and low TBARS value (~0.4 mmol malondialdehyde equivalents, MDAE/Kg oil), but high p-anisidine value (23–32). The results indicated that both Prunus avium L. and Prunus spinosa L. kernel oils exhibited unique chemical compositions.
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Kavaz Yuksel, Arzu. "The Effects of Blackthorn (P runus Spinosa L.) Addition on Certain Quality Characteristics of Ice Cream." Journal of Food Quality 38, no. 6 (2015): 413–21. http://dx.doi.org/10.1111/jfq.12170.
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Kotsou, Konstantina, Magdalena Stoikou, Vassilis Athanasiadis, et al. "Enhancing Antioxidant Properties of Prunus spinosa Fruit Extracts via Extraction Optimization." Horticulturae 9, no. 8 (2023): 942. http://dx.doi.org/10.3390/horticulturae9080942.
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Prunus spinosa fruit, commonly known as blackthorn or sloe, possesses a wide range of health-promoting properties, including antioxidant and antibacterial activities. In this study, we investigated the effects of various extraction parameters, such as temperature, time, and solvent composition, on the extraction of bioactive compounds from P. spinosa fruit. Response surface methodology was employed to optimize these parameters and evaluate their impact on the antioxidant properties of the extracts. Furthermore, techniques such as ultrasound (US) and pulsed electric field (PEF) were applied, both individually and in combination, to explore their potential effects on the extraction process. The optimal extraction technique proved to be the combination of PEF and US, followed by stirring. The most suitable solvent was 75% ethanol, whereas the optimum extraction time and temperature were 30 min and 80 °C, respectively. Our findings revealed that under the optimum extraction parameters, a significant improvement in the extraction efficiency of bioactive compounds from P. spinosa fruit was achieved. More specifically, the optimal conditions, according to partial least squares (PLS) analysis, were a combination of all three extraction modes (PEF‒US‒ST), the shorter extraction time of the present study (30 min), and the corresponding higher temperature (80 °C). As expected, the presence of ethanol was considered necessary, even in an amount of 25%. The total polyphenol content was found to be 30.74 mg gallic acid equivalent (GAE)/g, the total flavonoids content 3.23 mg rutin equivalents (RtE)/g and the total anthocyanins 125.2 μg cyanidin-3-O-glucoside equivalents (CyE)/g. HPLC-DAD analysis showed that neochlorogenic acid was the polyphenol with the highest concentration (4.13 mg GAE/g) in P. spinosa fruit. The antioxidant activity of the optimized, according to PLS analysis, extract was evaluated and found to be 146.09 μmol ascorbic acid equivalent (AAE)/g determined by ferric reducing antioxidant power (FRAP) assay, and by the radical scavenging activity (DPPH) assay was 18.56 μmol AAE/g. Additionally, the ascorbic acid was determined to be 119.4 mg/100 g. Overall, this study contributes valuable insights into the extraction optimization process and the potential applications of P. spinosa fruit in the development of functional foods and pharmaceutical products.
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Eremin, G. V. "The role of Prunus cerasifera Ehrh. in the origin, evolution and improvement of stone fruit cultivars." Proceedings on applied botany, genetics and breeding 181, no. 4 (2021): 136–43. http://dx.doi.org/10.30901/2227-8834-2020-4-136-143.
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At Krymsk Experiment Breeding Station of VIR, as a result of collecting, studying, and selective use of myrobalan plum genetic diversity, the intraspecific taxonomy of the species Prunus cerasifera Ehrh. was clarified. Donors of traits significant for breeding were identified and, with their use, adaptable cultivars of myrobalan plum, Russian plum and clonal rootstocks of stone fruit plants were developed.Intraspecific classification of P. cerasifera is proposed, with the identified subspecies:subsp. cerasifera (typical myrobalan plum);subsp. orientalis (M. Pop.) Erem. et Garcov. (Oriental myrobalan plum);and subsp. macrocarpa Erem. et Garcov. (large-fruited myrobalan plum).Within subsp. P. cerasifera subsp. macrocarpa, varieties were identified:var. macrocarpa (typical, or Krymsk);var. georgica Erem. et Garcov. (Georgian);var. iranica (Koval.) Erem. et Garcov. (Iranian);var. nairica (Koval.) Erem. et Garcov. (Armenian);var. pissardii Carr. (Pissard);and var. taurica (Kost.) Erem. et Garcov. (Taurida).The participation of myrobalan plum in the origin of spp. P. brigantiaca Vill., P. cocomilia Tem., and P. spinosa L. was traced. Involvement of large-fruited myrobalan plum in hybridization with blackthorn contributed to the manifestation of an exceptional polymorphism among the varieties of the hybridogenic species P. domestica L. (P. cerasifera × P. spinosa). As a result of hybridization between myrobalan plum and Chinese plum varieties, a new cultigen species emerged – Russian plum (× P. rossica Erem.). By now, 127 cultivars of this species and 7 clonal rootstocks for stone fruit crops have been developed. These cultivars and clonal rootstocks are zoned for Russia and a number of other countries.