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1

Richards, Gary P., Michael A. Watson, David S. Needleman, Karlee M. Church, and Claudia C. Häse. "Mortalities of Eastern and Pacific Oyster Larvae Caused by the Pathogens Vibrio coralliilyticus and Vibrio tubiashii." Applied and Environmental Microbiology 81, no. 1 (2014): 292–97. http://dx.doi.org/10.1128/aem.02930-14.

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ABSTRACTVibrio tubiashiiis reported to be a bacterial pathogen of larval Eastern oysters (Crassostrea virginica) and Pacific oysters (Crassostrea gigas) and has been associated with major hatchery crashes, causing shortages in seed oysters for commercial shellfish producers. Another bacterium,Vibrio coralliilyticus, a well-known coral pathogen, has recently been shown to elicit mortality in fish and shellfish. Several strains ofV. coralliilyticus, such as ATCC 19105 and Pacific isolates RE22 and RE98, were misidentified asV. tubiashiiuntil recently. We compared the mortalities caused by twoV.
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2

SU, YI-CHENG, QIANRU YANG, and CLAUDIA HÄSE. "Refrigerated Seawater Depuration for Reducing Vibrio parahaemolyticus Contamination in Pacific Oyster (Crassostrea gigas)." Journal of Food Protection 73, no. 6 (2010): 1111–15. http://dx.doi.org/10.4315/0362-028x-73.6.1111.

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The efficacy of refrigerated-seawater depuration for reducing Vibrio parahaemolyticus levels in Pacific oyster (Crassostrea gigas) was investigated. Raw Pacific oysters were inoculated with a mixed culture of five clinical strains of V. parahaemolyticus (105 to 106 most probable number [MPN] per g) and depurated with refrigerated seawater (5°C) in a laboratory-scale recirculation system equipped with a 15-W gamma UV sterilizer. Depuration with refrigerated seawater for 96 h reduced V. parahaemolyticus populations by >3.0 log MPN/g in oysters harvested in the winter. However, 144 h of de
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3

Markert, Alexandra. "How dense is dense? Toward a harmonized approach to characterizing reefs of non-native Pacific oysters – with consideration of native mussels." NeoBiota 57 (May 29, 2020): 7–52. http://dx.doi.org/10.3897/neobiota.57.49196.

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Pacific oysters Crassostrea (Magallana) gigas have been successfully invading ecosystems worldwide. As an ecosystem engineer, they have the potential to substantially impact on other species and on functional processes of invaded ecosystems. Engineering strength depends on oyster density in space and time. Density has not yet been studied on the extent of reef structural dynamics. This study assessed abundance of naturalized Pacific oysters by shell length (SL) of live individuals and post-mortem shells at six sites over six consecutive years during post-establishment. Individual biomass, i.e.
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4

Qurani, Risma, Fredinan Yulianda, and Agustinus Mangaratua Samosir. "Spatial Distribution of Pacific Oyster (Crassostrea gigas) Population Related Environment Factor in Coastal Water of Pabean Ilir, Indramayu." Jurnal Moluska Indonesia 4, no. 1 (2020): 38–47. http://dx.doi.org/10.54115/jmi.v4i1.12.

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Pacific oysters (Crassostrea gigas, Thunberg, 1793) is a benthic organisme that tend to live and settle in the bottom. One of the pacific oyster habitat is Coastal Water of Pabean Ilir, Indramayu. The purpose of this study was to map spatial condition of the population related habitat of the oyster (Crassostrea gigas). The mapping were done with laptop, using Arc GIS. There were 15 points of sampling. The oyster population in Pabean Ilir can be categorized into three categories: low, medium, and high density. Based on the similarity of environmental characteristics the habitat were divided int
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5

Boullot, Floriane, Caroline Fabioux, Hélène Hégaret, Pierre Boudry, Philippe Soudant, and Evelyne Benoit. "Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin." Marine Drugs 19, no. 7 (2021): 380. http://dx.doi.org/10.3390/md19070380.

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Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to analyse the PST-sensitivity of nerves of Pacific oysters in relation with toxin bio-accumulation. The results show that C. gigas nerves have micromolar range of saxitoxin (STX) sensitivity, thus providing intermediate STX sensitivity compared to other bivalve species. However, theses nerves were much less sensitive to tetr
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6

Li, Xing-Xia, Wen-Chao Yu, Zhong-Qiang Cai, et al. "Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific OysterCrassostrea gigas." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/5986519.

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The shell of the pearl oyster (Pinctada fucata) mainly comprises aragonite whereas that of the Pacific oyster (Crassostrea gigas) is mainly calcite, thereby suggesting the different mechanisms of shell formation between above two mollusks. Calmodulin (CaM) is an important gene for regulating the uptake, transport, and secretion of calcium during the process of shell formation in pearl oyster. It is interesting to characterize the CaM in oysters, which could facilitate the understanding of the different shell formation mechanisms among mollusks. We cloned the full-length cDNA of Pacific oyster
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7

Agnew, M. Victoria, Carolyn S. Friedman, Christopher Langdon, et al. "Differential Mortality and High Viral Load in Naive Pacific Oyster Families Exposed to OsHV-1 Suggests Tolerance Rather than Resistance to Infection." Pathogens 9, no. 12 (2020): 1057. http://dx.doi.org/10.3390/pathogens9121057.

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Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant,
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8

Lapègue, Sylvie, Serge Heurtebise, Florence Cornette, Erwan Guichoux, and Pierre-Alexandre Gagnaire. "Genetic Characterization of Cupped Oyster Resources in Europe Using Informative Single Nucleotide Polymorphism (SNP) Panels." Genes 11, no. 4 (2020): 451. http://dx.doi.org/10.3390/genes11040451.

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The Pacific oyster, Crassostrea gigas, was voluntarily introduced from Japan and British Columbia into Europe in the early 1970s, mainly to replace the Portuguese oyster, Crassostrea angulata, in the French shellfish industry, following a severe disease outbreak. Since then, the two species have been in contact in southern Europe and, therefore, have the potential to exchange genes. Recent evolutionary genomic works have provided empirical evidence that C. gigas and C. angulata exhibit partial reproductive isolation. Although hybridization occurs in nature, the rate of interspecific gene flow
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9

FERNANDEZ-PIQUER, JUDITH, JOHN P. BOWMAN, TOM ROSS, SILVIA ESTRADA-FLORES, and MARK L. TAMPLIN. "Preliminary Stochastic Model for Managing Vibrio parahaemolyticus and Total Viable Bacterial Counts in a Pacific Oyster (Crassostrea gigas) Supply Chain." Journal of Food Protection 76, no. 7 (2013): 1168–78. http://dx.doi.org/10.4315/0362-028x.jfp-12-401.

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Vibrio parahaemolyticus can accumulate and grow in oysters stored without refrigeration, representing a potential food safety risk. High temperatures during oyster storage can lead to an increase in total viable bacteria counts, decreasing product shelf life. Therefore, a predictive tool that allows the estimation of both V. parahaemolyticus populations and total viable bacteria counts in parallel is needed. A stochastic model was developed to quantitatively assess the populations of V. parahaemolyticus and total viable bacteria in Pacific oysters for six different supply chain scenarios. The
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10

Renault, Tristan, Anne Lise Bouquet, Julien-Thomas Maurice, Coralie Lupo, and Philippe Blachier. "Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality." Applied and Environmental Microbiology 80, no. 17 (2014): 5419–26. http://dx.doi.org/10.1128/aem.00484-14.

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ABSTRACTA number of bivalve species worldwide, including the Pacific oyster,Crassostrea gigas, have been affected by mass mortality events associated with herpesviruses, resulting in significant losses. A particular herpesvirus was purified from naturally infected larval Pacific oysters, and its genome was completely sequenced. This virus has been classified asOstreid herpesvirus 1(OsHV-1) within the familyMalacoherpesviridae. Since 2008, mass mortality outbreaks amongC. gigasin Europe have been related to the detection of a variant of OsHV-1 called μVar. Additional data are necessary to bette
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11

TAKAHASHI, MICHIKO, YUMIKO OKAKURA, HAJIME TAKAHASHI, et al. "Evaluation of Inactivation of Murine Norovirus in Inoculated Shell Oysters by High Hydrostatic Pressure Treatment." Journal of Food Protection 82, no. 12 (2019): 2169–73. http://dx.doi.org/10.4315/0362-028x.jfp-19-186.

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ABSTRACT One of the major foods causing norovirus gastroenteritis is bivalve shellfish, such as oysters. Depuration and relaying methods have been used to control norovirus. However, these methods may be inadequate to control norovirus gastroenteritis. The present study aimed to investigate the effectiveness of high hydrostatic pressure (HHP) treatment in controlling norovirus in shelled oysters, by evaluating the inactivating effect of HHP on murine norovirus strain 1 (MNV-1) inoculated into a buffer, oyster homogenate, and shelled oysters. First, MNV-1 was inoculated (infectivity of 4.5 log
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12

Potts, Robert W. A., Alejandro P. Gutierrez, Yennifer Cortés-Araya, Ross D. Houston, and Tim P. Bean. "Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas." PeerJ 8 (June 1, 2020): e9180. http://dx.doi.org/10.7717/peerj.9180.

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Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture
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13

Dhanji-Rapkova, Monika, Andrew D. Turner, Craig Baker-Austin, Jim F. Huggett, and Jennifer M. Ritchie. "Distribution of Tetrodotoxin in Pacific Oysters (Crassostrea gigas)." Marine Drugs 19, no. 2 (2021): 84. http://dx.doi.org/10.3390/md19020084.

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A potent and heat-stable tetrodotoxin (TTX) has been found to accumulate in various marine bivalve species, including Pacific oysters (Crassostrea gigas), raising a food safety concern. While several studies on geographical occurrence of TTX have been conducted, there is a lack of knowledge about the distribution of the toxin within and between bivalves. We, therefore, measured TTX in the whole flesh, mantle, gills, labial palps, digestive gland, adductor muscle and intravalvular fluid of C. gigas using liquid chromatography-tandem mass spectrometry. Weekly monitoring during summer months reve
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14

Aagesen, Alisha M., Sureerat Phuvasate, Yi-Cheng Su, and Claudia C. Häse. "Persistence of Vibrio parahaemolyticus in the Pacific Oyster, Crassostrea gigas, Is a Multifactorial Process Involving Pili and Flagella but Not Type III Secretion Systems or Phase Variation." Applied and Environmental Microbiology 79, no. 10 (2013): 3303–5. http://dx.doi.org/10.1128/aem.00314-13.

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ABSTRACTVibrio parahaemolyticuscan resist oyster depuration, suggesting that it possesses specific factors for persistence. We show that type I pili, type IV pili, and both flagellar systems contribute toV. parahaemolyticuspersistence in Pacific oysters whereas type III secretion systems and phase variation do not.
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15

ABEYTA, CARLOS, FRANK G. DEETER, CHARLES A. KAYSNER, ROBERT F. STOTT, and MARLEEN M. WEKELL. "Campylobacter jejuni in a Washington State Shellfish Growing Bed Associated With Illness." Journal of Food Protection 56, no. 4 (1993): 323–25. http://dx.doi.org/10.4315/0362-028x-56.4.323.

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Consumption of raw Pacific oysters (Crassotea gigas) harvested from a Washington State recreational shellfish bed were associated with illness. Illness occurred within 2 d of ingestion of a half-dozen shellstock oysters. Each oyster consist of approximately 20 g of meat. The duration of illness lasted 2 d. Routinely, Campylobacter species have been found in several shellfish beds in the Puget Sound Bay. Its presence in the marine environment appears to be incidental and primarily, comes from wild birds, farm runoff, and sewage bypasses. This paper describes the first reported case of Campyloba
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16

Vyalova, O. Yu. "Growth and terms of obtaining marketable triploid oysters in Donuzlav Liman (Black Sea, Crimea)." Marine Biological Journal 4, no. 1 (2019): 24–32. http://dx.doi.org/10.21072/mbj.2019.04.1.03.

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The rate of linear and weight growth of triploid spat of oyster Crassostrea gigas in Donuzlav Liman (Black Sea, Crimea) was investigated. A quantitative relationship between the mass (W, g) of the whole oyster and the height of the shell (H, mm) is given (it is described by the equation W = 7 · 10-5 · Н3.1, R² = 0.78), as well as a quantitative relationship between the mass (W, g) of the whole oyster and the length of the shell (L, mm) (W = 3 · 10-4 · L3.12, R² = 0.65). The maximum rates of linear growth of triploid oysters T20 were registered in April (1.15 mm per day), of weight growth – in
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17

Cruz, C. D., D. Hedderley, and G. C. Fletcher. "Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish." Applied and Environmental Microbiology 81, no. 7 (2015): 2320–27. http://dx.doi.org/10.1128/aem.04020-14.

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ABSTRACTThe food-borne pathogenVibrio parahaemolyticushas been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence ofV. parahaemolyticusin NZ oysters and Greenshell mussels and the prevalence ofV. parahaemolyticustdhandtrhstrains. Pacific (235) and dredge (21) oyster samples and mussel samples (55) were obtained from commercial shellfish-growing areas between December 2009 and June 2012. TotalV. parahaemolyticusnumbers and the presence of pathoge
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18

TIAN, PENG, ANNA L. ENGELBREKTSON, and ROBERT E. MANDRELL. "Seasonal Tracking of Histo-Blood Group Antigen Expression and Norovirus Binding in Oyster Gastrointestinal Cells." Journal of Food Protection 71, no. 8 (2008): 1696–700. http://dx.doi.org/10.4315/0362-028x-71.8.1696.

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Noroviruses (NORs) are the most common cause of viral gastroenteritis outbreaks. Outbreaks are often associated with the consumption of contaminated oysters and generally occur between the months of November and March, when oysters produce the highest levels of glycogen. Oyster glycogen has been proposed as playing a role in NOR accumulation. Recent research indicates that histo-blood group antigens (HBGAs) function as viral receptors on human gastrointestinal cells. In this study, oyster glycogen was tested to determine whether it contains HBGA-like molecules and whether it plays a role in NO
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Batley, GE, C. Fuhua, CI Brockbank, and KJ Flegg. "Accumulation of Tributyltin by the Sydney Rock Oyster, Saccostrea commercialis." Marine and Freshwater Research 40, no. 1 (1989): 49. http://dx.doi.org/10.1071/mf9890049.

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Tributyltin (TBT) concentrations have been measured in the tissue of the Sydney rock oyster Saccostrea commercialis sampled from estuaries in New South Wales, Australia. Background TBT levels of below 2 ng Sn g-1 contrasted with values between 80 and 130 ng Sn g-1 in oysters exposed to high boat densities or poor tidal flushing. Shell deformities and reduced tissue weights were associated with all samples displaying elevated TBT levels. Specimens of the Pacific oyster, Crassostrea gigas, growing on the same racks displayed 2-3 times the TBT concentrations of S. commercialis.
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Sakamaki, T., K. Hayashi, Y. Zheng, M. Fujibayashi, and O. Nishimura. "Effects of oyster age on selective suspension-feeding and the chemical composition of biodeposits: insights from fatty acid analysis." Marine Ecology Progress Series 644 (June 25, 2020): 75–89. http://dx.doi.org/10.3354/meps13359.

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The study objective was to clarify how the growth stages of the Pacific oyster Crassostrea gigas affect selective suspension-feeding of particulate organic matter (POM) and the composition of biodeposits. A day-long (22 h), continuous-flow mesocosm experiment was conducted with 3, 15, and 27 mo old oysters. The suspended particulate matter (PM), settled PM (mostly biodeposits in the oyster mesocosms), and oyster soft tissues were analysed to determine the content of fatty acids, organic carbon, and nitrogen, as well as the carbon and nitrogen stable isotope ratios to trace compositional change
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21

Li, Yan, Jian G. Qin, Catherine A. Abbott, Xiaoxu Li, and Kirsten Benkendorff. "Synergistic impacts of heat shock and spawning on the physiology and immune health ofCrassostrea gigas: an explanation for summer mortality in Pacific oysters." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 293, no. 6 (2007): R2353—R2362. http://dx.doi.org/10.1152/ajpregu.00463.2007.

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Mass mortality is often observed in cultured oysters during the period following spawning in the summer season. To examine the underlying causes leading to this phenomenon, thermotolerance of the Pacific oyster Crassostrea gigas was assessed using pre- and postspawning oysters that were sequentially treated with sublethal (37°C) and lethal heat shocks (44°C). The effects were examined on a range of immune and metabolic parameters in addition to mortality rate. A preventative 37°C significantly reduced oyster mortality after exposure to a second heat shock of 44°C, but in postspawning oysters m
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Rupnik, Agnieszka, William Doré, Leon Devilly, et al. "Evaluation of Norovirus Reduction in Environmentally Contaminated Pacific Oysters During Laboratory Controlled and Commercial Depuration." Food and Environmental Virology 13, no. 2 (2021): 229–40. http://dx.doi.org/10.1007/s12560-021-09464-2.

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AbstractNorovirus contamination of oysters is the lead cause of non-bacterial gastroenteritis and a significant food safety concern for the oyster industry. Here, norovirus reduction from Pacific oysters (Crassostrea gigas), contaminated in the marine environment, was studied in laboratory depuration trials and in two commercial settings. Norovirus concentrations were measured in oyster digestive tissue before, during and post-depuration using the ISO 15216-1 quantitative real-time RT-PCR method. Results of the laboratory-based studies demonstrate that statistically significant reductions of u
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Pernet, Fabrice, Sonia Gachelin, Jean-Yves Stanisière, Bruno Petton, Elodie Fleury, and Joseph Mazurié. "Farmer monitoring reveals the effect of tidal height on mortality risk of oysters during a herpesvirus outbreak." ICES Journal of Marine Science 76, no. 6 (2019): 1816–24. http://dx.doi.org/10.1093/icesjms/fsz074.

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Abstract The intertidal zone is characterized by a sharp vertical gradient of environmental stress, which structures species distribution and their interactions. Few studies, however, have examined the influence of tidal height on host–pathogen interactions. Here, we investigated how the tidal height influence outbreak of the Ostreid herpesvirus type 1 (OsHV-1) affecting the Pacific oyster. A volunteer network composed of 20 oyster growers monitored the survival of 28 batches of oysters during an epizootic event in Southern Brittany, France. Oysters were spat from wild collection or hatchery p
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Grano-Maldonado, M. I., F. Rubalcava-Ramirez, A. Rodriguez-Santiago, F. Garcia-Vargas, A. Medina-Jasso, and M. Nieves-Soto. "First record of Stephanostomum sp. Looss, 1899 (Digenea: Acanthocolpidae) metacercariae parasitising the pleasure oyster Crassostrea corteziensis (Hertlein) from the Mexican Pacific coast." Helminthologia 56, no. 3 (2019): 211–18. http://dx.doi.org/10.2478/helm-2019-0019.

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SummaryThe aim of this investigation was to identify the parasites present in the largely understudied pleasure oyster Crassostrea corteziensis in Sinaloa state in the northwestern Mexican Pacific coast. Inspection of twenty-eight oysters collected on “Ceuta” lagoon revealed the presence of the digenean Stephanostomum sp. (Digenea: Acanthocolpidae) cysts. Metacercariae were found encapsulated and embedded in the digestive gland and mantle tissue of oysters. The prevalence of infection revealed that 84.6 % were infected, the abundance was 13.62, with a mean intensity of 16.09 per host. The memb
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Steele, S., and M. F. Mulcahy. "Gametogenesis of the oyster Crassostrea gigas in southern Ireland." Journal of the Marine Biological Association of the United Kingdom 79, no. 4 (1999): 673–86. http://dx.doi.org/10.1017/s0025315498000836.

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The Pacific oyster Crassostrea gigas (Mollusca: Bivalvia) was introduced to Ireland in 1965 and is farmed at many sites around the coast. The reproductive biology of 1377 oysters from two sites on the south coast of Ireland was examined from April 1996 until December 1997 for variations in maturation rate and condition indices. Qualitative data were compiled by staging gonadal development using histological sections. Environmental parameters of temperature, dissolved oxygen and chlorophyll-a levels, as well as parasites and pathology were monitored. Unusually high sea temperatures led to oyste
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Bishop, Melanie J., Fredrick R. Krassoi, Ross G. McPherson, et al. "Change in wild-oyster assemblages of Port Stephens, NSW, Australia, since commencement of non-native Pacific oyster (Crassostrea gigas) aquaculture." Marine and Freshwater Research 61, no. 6 (2010): 714. http://dx.doi.org/10.1071/mf09177.

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Proliferation of species introduced for aquaculture can threaten the ecological and economic integrity of ecosystems. We assessed whether the non-native Pacific oyster, Crassostrea gigas, has proliferated, spread and overgrown native Sydney rock oysters, Saccostrea glomerata, in Port Stephens, New South Wales (NSW), Australia, following the 1991 decision to permit its aquaculture within this estuary. Sampling of seven rocky-shore and four mangrove sites immediately before (1990), immediately after (1991–1992) and nearly two decades after (2008) the commencement of C. gigas aquaculture did not
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SU, LAIJIN, LIPING MA, HUI LIU, FENG ZHAO, ZHIWEI SU, and DEQING ZHOU. "Presence and Distribution of Histo-Blood Group Antigens in Pacific Oysters and the Effects of Exposure to Noroviruses GI.3 and GII.4 on Their Expression." Journal of Food Protection 81, no. 11 (2018): 1783–90. http://dx.doi.org/10.4315/0362-028x.jfp-18-074.

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ABSTRACT Noroviruses (NoVs) are one of the most important foodborne viral pathogens worldwide. Oysters are common carriers of NoVs and are responsible for their transmission. NoVs recognize human histo-blood group antigens (HBGAs) as receptors. Recent studies indicate that HBGA-like molecules also exist in oyster tissues and that they may play a key role in the binding of NoVs. However, the mechanism by which different genotypes of NoV accumulate in different oyster tissues is unknown. In this study, the presence and distribution of different types of HBGA-like molecules were evaluated in 240
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Mason, CJ, and JA Nell. "Condition index and chemical composition of meats of Sydney rock oysters (Saccostrea commercialis) and Pacific oysters (Crassostrea gigas) at four sites in Port Stephens, NSW." Marine and Freshwater Research 46, no. 5 (1995): 873. http://dx.doi.org/10.1071/mf9950873.

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Adult Sydney rock oysters (Saccostrea commercialis) and Pacific oysters (Crassostrea gigas) were kept on commercial oyster leases at three intertidal sites in Port Stephens, New South Wales, and subtidally under an experimental raft at a fourth site between July 1988 and September 1989. Oysters were sampled from each site at approximately monthly intervals for chemical and histological analysis. Condition index and percentage glycogen of Pacific oysters were higher than those of Sydney rock oysters during winter and spring but tended to be lower during summer and autumn. Gonads of Pacific oyst
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Humphrey, T. J., and K. Martin. "Bacteriophage as models for virus removal from Pacific oysters (Crassostrea gigas) during re-laying." Epidemiology and Infection 111, no. 2 (1993): 325–35. http://dx.doi.org/10.1017/s0950268800057034.

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SummaryA study was undertaken to examine the feasibility of using naturally-occurring bacteriophages to assess the impact of re-laying on levels of viral contamination inCrassostrea gigas, the Pacific oyster. Two phages were chosen. One, male-specific (F+), was enumerated usingSalmonella typhimurium. The other, a somatic phage, was detected using an, as yet, uncharacterizedEscherichia coli. Investigations, using a variety of re-laying sites, demonstrated that numbers of F+ phage in oyster tissue declined more rapidly than those of somatic phage. For example, in oysters placed in commercially-u
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Suquet, Marc, Goulwen de Kermoysan, Ricardo Gonzalez Araya, et al. "Anesthesia in Pacific oyster,Crassostrea gigas." Aquatic Living Resources 22, no. 1 (2009): 29–34. http://dx.doi.org/10.1051/alr/2009006.

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Wilkie, Emma M., and Melanie J. Bishop. "Differences in shell strength of native and non-native oysters do not extend to size classes that are susceptible to a generalist predator." Marine and Freshwater Research 63, no. 12 (2012): 1201. http://dx.doi.org/10.1071/mf12078.

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Whether non-native species proliferate to pest status can depend on top-down control from native predators. Among epifaunal molluscs, the shell serves as a main line of anti-predator defence and shell strength may determine the ease with which generalist predators can penetrate prey. We assessed whether, in eastern Australia, the faster growth of the non-native Pacific oyster, Crassostrea gigas, than that of the native Saccostrea glomerata comes at the cost of reduced investment in shell thickening, rendering the non-native oyster more susceptible to a generalist predator, the muricid gastropo
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Kovalyova, M. A., and O. Yu Vyalova. "The first record of rock-boring mollusc Petricola lithophaga (Retzius, 1788) inside the valves of oysters Crassostrea gigas (Thunberg, 1793), cultivated in Crimea (the Donuzlav Bay, the Black Sea)." Marine Biological Journal 6, no. 1 (2021): 34–40. http://dx.doi.org/10.21072/mbj.2021.06.1.03.

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The number of mollusc farms off the coast of Crimea and the Caucasus has increased significantly in recent years. The cultivation of the Pacific oyster Crassostrea gigas (Thunberg, 1793) requires monitoring of mollusc health and parasitological control of mariculture farms. The aim of this work was to study species composition of epibionts and endobionts, associated with shells of cultivated oyster C. gigas, as well as to identify species, damaging shells. Commercial oysters with visual shell damage were collected on a mariculture farm in the Donuzlav Bay (Crimea, the Black Sea) and brought to
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Bouilly, Karine, Alexandra Leitão, Raquel Chaves, Henrique Guedes-Pinto, Pierre Boudry, and Sylvie Lapègue. "Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas." Genome 48, no. 1 (2005): 177–80. http://dx.doi.org/10.1139/g04-087.

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Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we use
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Launey, Sophie, and Dennis Hedgecock. "High Genetic Load in the Pacific Oyster Crassostrea gigas." Genetics 159, no. 1 (2001): 255–65. http://dx.doi.org/10.1093/genetics/159.1.255.

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Abstract The causes of inbreeding depression and the converse phenomenon of heterosis or hybrid vigor remain poorly understood despite their scientific and agricultural importance. In bivalve molluscs, related phenomena, marker-associated heterosis and distortion of marker segregation ratios, have been widely reported over the past 25 years. A large load of deleterious recessive mutations could explain both phenomena, according to the dominance hypothesis of heterosis. Using inbred lines derived from a natural population of Pacific oysters and classical crossbreeding experiments, we compare th
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LYNCH, S. A., E. ABOLLO, A. RAMILO, A. CAO, S. C. CULLOTY, and A. VILLALBA. "Observations raise the question if the Pacific oyster, Crassostrea gigas, can act as either a carrier or a reservoir for Bonamia ostreae or Bonamia exitiosa." Parasitology 137, no. 10 (2010): 1515–26. http://dx.doi.org/10.1017/s0031182010000326.

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SUMMARYThis study investigated the ability of the Pacific oyster, Crassostrea gigas, to act as a carrier or reservoir of the protistan Bonamia ostreae. Studies were carried out independently in Ireland and in Spain. Naïve C. gigas were exposed to B. ostreae both in the field and in the laboratory via natural exposure or experimental injection. Naïve flat oysters, Ostrea edulis, were placed in tanks with previously exposed C. gigas. Oysters were screened for B. ostreae by examination of ventricular heart smears and by polymerase chain reaction (PCR) screening of tissue samples (gill and/or hear
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Hwang, Dukhyun, Min-jae Kang, Mi Jo, Yong Seo, Nam Park та Gun-Do Kim. "Anti-Inflammatory Activity of β-thymosin Peptide Derived from Pacific Oyster (Crassostrea gigas) on NO and PGE2 Production by Down-Regulating NF-κB in LPS-Induced RAW264.7 Macrophage Cells". Marine Drugs 17, № 2 (2019): 129. http://dx.doi.org/10.3390/md17020129.

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β-thymosin is known for having 43 amino acids, being water-soluble, having a light molecular weight and ubiquitous polypeptide. The biological activities of β-thymosin are diverse and include the promotion of wound healing, reduction of inflammation, differentiation of T cells and inhibition of apoptosis. Our previous studies showed that oyster β-thymosin originated from the mantle of the Pacific oyster, Crassostrea gigas and had antimicrobial activity. In this study, we investigated the anti-inflammatory effects of oyster β-thymosin in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell
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Gray, Matthew W., and Chris J. Langdon. "Ecophysiology of the Olympia Oyster, Ostrea lurida, and Pacific Oyster, Crassostrea gigas." Estuaries and Coasts 41, no. 2 (2017): 521–35. http://dx.doi.org/10.1007/s12237-017-0273-7.

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Douillet, P. "Bacterivory in Pacific oyster Crassostrea gigas larvae." Marine Ecology Progress Series 98 (1993): 123–34. http://dx.doi.org/10.3354/meps098123.

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Park, Mi Seon, Byung Hwa Min, Jung Jun Park, Hyun Jeong Lim, Jeong-In Myeong, and Min Hwan Jeong. "Cryopreservation of Pacific Oyster, Crassostrea gigas Sperm." Korean Journal of Malacology 29, no. 3 (2013): 251–58. http://dx.doi.org/10.9710/kjm.2013.29.3.251.

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Friedman, Carolyn S., Deborah F. Cloney, Donald Manzer, and Ronald P. Hedrick. "Haplosporidiosis of the Pacific oyster, Crassostrea gigas." Journal of Invertebrate Pathology 58, no. 3 (1991): 367–72. http://dx.doi.org/10.1016/0022-2011(91)90182-p.

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EZGETA BALIĆ, DARIA, IVANA RADONIĆ, DUBRAVKA BOJANIĆ VAREZIĆ, et al. "Reproductive cycle of a non-native oyster, Crassostrea gigas, in the Adriatic Sea." Mediterranean Marine Science 21, no. 1 (2020): 146. http://dx.doi.org/10.12681/mms.21304.

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The Pacific oyster Crassostrea gigas was introduced for the aquaculture purposes in many different parts of the world. However, the species has never been officially introduced for commercial farming in the Croatian part of the Adriatic Sea. Interestingly, in the 1970s, the Pacific oyster has been reported in the natural habitats of the Lim Bay, in northern Adriatic Sea. Although the species was recorded there, there is a lack of knowledge about its biology and ecology in this region, including a reproductive cycle. Information on the reproductive biology of non-indigenous species in new areas
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Fu, Huiru, Zexin Jiao, Yongjing Li, et al. "Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata." International Journal of Molecular Sciences 22, no. 6 (2021): 3222. http://dx.doi.org/10.3390/ijms22063222.

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Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster (Crassostrea gigas) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas, which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generat
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Gomes, Renata Bezerra, Patrick Rafael DYBAS, Francisco Carlos SILVA, Simone SÜHNEL, Marcos Caivano Pedroso ALBUQUERQUE, and Claudio Manoel Rodrigues MELO. "Estimates of genetic parameters for growth and survival in pacific oyster (Crassostrea gigas)." Boletim do Instituto de Pesca 44, no. 2 (2018): 337. http://dx.doi.org/10.20950/1678-2305.2018.337.

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Cilenti, Lucrezia, Tommaso Scirocco, Antonietta Specchiulli, et al. "Quality aspects of Crassostrea gigas (Thunberg, 1793) reared in the Varano Lagoon (southern Italy) in relation to marketability." Journal of the Marine Biological Association of the United Kingdom 98, no. 1 (2017): 71–79. http://dx.doi.org/10.1017/s0025315417001692.

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Shellfish culture, based on ecological and market assessment, is considered a driving force for socio-economic change in ecologically complex coastal systems such as lagoons throughout the Mediterranean area. To diversify fish production, the Pacific oyster Crassostrea gigas was cultured at commercial farms in the Varano Lagoon (SE Italy). The aims of this study were to evaluate through four condition indices (CI, CICG, CIE and AFNOR index), the Polydora index (PI), lipid content, quality and market aspects of oysters reared at two different sites (FO and LA) of the Varano Lagoon, which are ch
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Copes, Ray, Nina Annika Clark, Karen Rideout, Jan Palaty, and Kay Teschke. "Uptake of cadmium from Pacific oysters (Crassostrea gigas) in British Columbia oyster growers." Environmental Research 107, no. 2 (2008): 160–69. http://dx.doi.org/10.1016/j.envres.2008.01.014.

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Li, Yongjing, Huiru Fu, Fuqiang Zhang, et al. "Insulin-Like Peptide Receptor-Mediated Signaling Pathways Orchestrate Regulation of Growth in the Pacific Oyster (Crassostrea gigas), as Revealed by Gene Expression Profiles." International Journal of Molecular Sciences 22, no. 10 (2021): 5259. http://dx.doi.org/10.3390/ijms22105259.

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The involvement of insulin/insulin-like growth factor signaling (IIS) pathways in the growth regulation of marine invertebrates remains largely unexplored. In this study, we used a fast-growing Pacific oyster (Crassostrea gigas) variety “Haida No.1” as the material with which to unravel the role of IIS systems in growth regulation in oysters. Systematic bioinformatics analyses allowed us to identify major components of the IIS signaling pathway and insulin-like peptide receptor (ILPR)-mediated signaling pathways, including PI3K-AKT, RAS-MAPK, and TOR, in C. gigas. The expression levels of the
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Chen, Yuan Yuan, Shun Sheng Chen, Wei Qiang Qiu, and Qiao Lu Li. "Identification of Different Varieties of Oyster Juice Based on the Comparison of Free Amino Acids." Advanced Materials Research 781-784 (September 2013): 1534–39. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.1534.

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To identify different varieties of oyster juice, a study was conducted to compare the free amino acids (FAAs) determined by automatic amino-acid analyzer in Southern oyster juice, Pacific oyster juice and Pearl oyster juice. In 18 amino acids, glutamic acids, glycine, alanine were considered to be the main flavor amino acids in oyster juice according to the method of Taste Activity Value (TAV). Serine, glycine, alanine, arginine, threonine and histidine proved to be the characteristic amino acids in three oyster juice with different Brix degrees. The standard characteristic amino acids content
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Gutierrez, Alejandro P., Frances Turner, Karim Gharbi, et al. "Development of a Medium Density Combined-Species SNP Array for Pacific and European Oysters (Crassostrea gigas and Ostrea edulis)." G3 Genes|Genomes|Genetics 7, no. 7 (2017): 2209–18. http://dx.doi.org/10.1534/g3.117.041780.

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Abstract SNP arrays are enabling tools for high-resolution studies of the genetic basis of complex traits in farmed and wild animals. Oysters are of critical importance in many regions from both an ecological and economic perspective, and oyster aquaculture forms a key component of global food security. The aim of our study was to design a combined-species, medium density SNP array for Pacific oyster (Crassostrea gigas) and European flat oyster (Ostrea edulis), and to test the performance of this array on farmed and wild populations from multiple locations, with a focus on European populations
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Huvet, A., P. Boudry, M. Ohresser, C. Delsert, and F. Bonhomme. "Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species." Animal Genetics 31, no. 1 (2000): 71–72. http://dx.doi.org/10.1111/j.1365-2052.2000.579-5.x.

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COOK, DAVID W., PAUL O'LEARY, JEFF C. HUNSUCKER, et al. "Vibrio vulnificus and Vibrio parahaemolyticus in U.S. Retail Shell Oysters: A National Survey from June 1998 to July 1999." Journal of Food Protection 65, no. 1 (2002): 79–87. http://dx.doi.org/10.4315/0362-028x-65.1.79.

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From June 1998 to July 1999, 370 lots of oysters in the shell were sampled at 275 different establishments (71%, restaurants or oyster bars; 27%, retail seafood markets; and 2%, wholesale seafood markets) in coastal and inland markets throughout the United States. The oysters were harvested from the Gulf (49%), Pacific (14%), Mid-Atlantic (18%), and North Atlantic (11%) Coasts of the United States and from Canada (8%). Densities of Vibrio vulnificus and Vibrio parahaemolyticus were determined using a modification of the most probable number (MPN) techniques described in the Food and Drug Admin
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