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Vincent, Olivier, Lynne Rainbow, Joan Tilburn, Herbert N. Arst,, and Miguel A. Peñalva. "YPXL/I Is a Protein Interaction Motif Recognized by Aspergillus PalA and Its Human Homologue, AIP1/Alix." Molecular and Cellular Biology 23, no. 5 (March 1, 2003): 1647–55. http://dx.doi.org/10.1128/mcb.23.5.1647-1655.2003.
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ABSTRACT The zinc finger transcription factor PacC undergoes two-step proteolytic activation in response to alkaline ambient pH. PalA is a component of the fungal ambient pH signal transduction pathway. Its mammalian homologue AIP1/Alix interacts with the apoptosis-linked protein ALG-2. We show that both PalA and AIP1/Alix recognize a protein-protein binding motif that we denote YPXL/I, where Tyr, Pro, and Leu/Ile are crucial for its interactive properties. Two such motifs flanking the signaling protease cleavage site mediate direct binding of PalA to PacC, required for the first and only pH-regulated cleavage of this transcription factor. PalA can bind the “closed” (i.e., wild-type full-length) conformer of PacC, suggesting that PalA binding constitutes the first stage in the two-step proteolytic cascade, recruiting or facilitating access of the signaling protease, presumably PalB. In addition to recognizing YPXL/I motifs, both PalA and AIP1/Alix interact with the Aspergillus class E Vps protein Vps32 homologue, a member of a protein complex involved in the early steps of the multivesicular body pathway, suggesting that this interaction is an additional feature of proteins of the PalA/AIP1/Alix family.
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Kropf, S. M., M. L. Putnam, M. Serdani, M. C. Twomey, J. L. Woods, and D. H. Gent. "Sclerotinia Wilt of Hop (Humulus lupulus) Caused by Sclerotinia sclerotiorum in the Pacific Northwest United States." Plant Disease 96, no. 4 (April 2012): 583. http://dx.doi.org/10.1094/pdis-12-11-1039.
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In June 2009, wilted hop bines were observed in a yard in Marion County, OR. The wilt was associated with a stem rot that occurred ~1 m from the ground near the point where bines are tied together for horticultural purposes. Samples of affected stems were submitted to the Oregon State University Plant Clinic. White hyphae and large, black sclerotia were present on the stems, with a clear delineation between healthy and diseased tissue. The pathogen was identified as Sclerotinia sclerotiorum based on morphological characters. In June 2011, bine wilting was observed on the same farm but in a different hop yard (cv. Nugget) ~10 km from the 2009 occurrence. Affected plants had upward curled leaves with necrotic margins or wilted bines that were severed at the soil line. Wilted bines tended to have smaller diameters than bines with foliar symptoms only. Of 100 plants examined, 75% displayed some foliar symptoms and 66% had at least one bine that was wilted. Yield loss was estimated at 10 to 20% due to bine wilting before cone development. Unlike the 2009 occurrence, wilted bines did not display aerial signs of S. sclerotiorum. Rather, water-soaked lesions covered in white, cottony mycelium were apparent on affected stems 2.5 to 5 cm below the soil surface, some bearing large, irregularly shaped sclerotia. Isolations made onto potato dextrose agar yielded isolates with rapid growth rates and morphological characters consistent with S. sclerotiorum (1). DNA was extracted (2) and pathogen identity was confirmed by PCR amplification and sequencing of the internal transcribed spacer regions from isolates SS001 and SS002 as described before (4). The amplicons were sequenced bidirectionally and consensus sequences were 100% similar to S. sclerotiorum (GenBank No. AAGT01000678.1). Two nucleotide polymorphisms were present that differentiated the sequences from those of 12 S. trifoliorum accessions in GenBank that could be aligned (2). Greenhouse assays utilizing a toothpick inoculation procedure (3) were conducted to fulfill Koch's postulates. Stems of five 4-week-old hop plants of cv. Agate were pierced with a toothpick colonized with S. sclerotiorum. Five control plants were similarly inoculated with toothpicks without the fungus. Inoculated plants developed symptoms similar to those observed in the field within 11 days; four of five plants inoculated with isolate SS001 and two of five plants inoculated with isolate SS002 completely wilted. S. sclerotiorum was reisolated from all inoculated plants but not the control plants. To our knowledge, this is the first report of Sclerotinia wilt on hop in Oregon or the Pacific Northwest (1), where nearly all commercial hop production occurs in the United States. The disease appears to be localized to a limited number of yards, although given the widespread distribution and host range of S. sclerotiorum, it is plausible that the disease may occur in other yards. Recurrent outbreaks and spread of the disease among yards on the affected farm suggests that Sclerotinia wilt has the potential to become a perennial problem on hop and efforts to limit the introduction of S. sclerotiorum into other yards are warranted. References: (1) D. H. Gent. Page 32 in: Compendium of Hop Diseases and Pests. The American Phytopathological Society, St. Paul, MN, 2009. (2) E. N. Njambere et al. Plant Dis. 92:917, 2008. (3) M. L. Putnam. Plant Pathol. 53:252, 2004. (4) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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Spapen, Jerrold, Stijn Lochy, and Vincent Michiels. "A shocking bike accident." Heart 104, no. 20 (July 20, 2018): 1706. http://dx.doi.org/10.1136/heartjnl-2018-313504.
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Clinical introductionA man in his late 40s presented with severe chest pain and progressive dyspnoea after hitting a tree at high speed during a bike ride in the woods. On admission, the patient appeared agitated, pale and sweaty. Core temperature was 35.5°C, respiratory rate 35/min and blood pressure 90/50 mm Hg with a regular pulse at 110 beats/min. Physical examination revealed multiple sternal bruises, distended jugular veins and muffled heart sounds (figure 1A). Lactate level was 4.4 mmol/L (normal <2.0 mmol/L) and high-sensitivity cardiac troponin T 0.142 mcg/L (normal <0.005 mcg/L). An ECG and a total body CT scan were performed (figure 1B,C).Figure 1(A) Multiple sternal bruises. (B) ECG on admission. (C) Thoracic CT.Question: What is the most likely diagnosis?Inferior myocardial infarction with right ventricular involvement.Cardiac contusion with tamponade.Commotio cordis.Aortic laceration.
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ZIEGLER, THOMAS, PATRICK DAVID, AURELIEN MIRALLES, DOAN VAN KIEN, and Nguyen Quang Truong. "A new species of the snake genus Fimbrios from Phong Nha-Ke Bang National Park, Truong Son, central Vietnam (Squamata: Xenodermatidae)." Zootaxa 1729, no. 1 (March 20, 2008): 37. http://dx.doi.org/10.11646/zootaxa.1729.1.4.
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A second species of the previously monotypic snake genus Fimbrios is described from the Truong Son Mountain Range, Vietnam. Fimbrios smithi sp. n. is included in this genus on the basis of the combination of the following characters: rostral, mental and first three to four labials with raised, erected edges; a horizontal ridge of tissues above the rostral; a very large loreal. It is distinguished from F. klossi by having the suture between the internasals longer than that between the prefrontals; two suboculars; 193 ventrals, and 72 unpaired subcaudal scales; total length of at least 440 mm in males (with a tail length of 94 mm, and a tail / total length ratio of 0.214); dorsum greyish brown, with a paler flank area, and pale blotches and stripes in the neck region. Fimbrios smithi sp. n. is the seventh snake species that has been described as new from the Phong Nha—Ke Bang National Park, Quang Binh Province, central Vietnam, in the last decade; it is the forty-fourth colubrid snake species known from that region, which now comprises 60 snake species in general.
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Wilcox, Melissa M. "Religio: The ties that bind." Critical Research on Religion 4, no. 3 (November 17, 2016): 286–91. http://dx.doi.org/10.1177/2050303216676526.
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Santana Suárez, Néstor. "¿Reprimarización en América Latina?: Efectos de la demanda china sobre el patrón exportador latinoamericano y las estructuras económicas internas (1995-2016)." Papeles de Europa 31, no. 2 (May 10, 2019): 149–74. http://dx.doi.org/10.5209/pade.63636.
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El objetivo de este trabajo es estudiar si la demanda china, dadas sus características propias, estaría incentivando una dinámica reprimarizadora de América Latina en el período 1995-2016. Para ello analizamos el patrón exportador de Argentina, Brasil, Chile, Cuba, Perú, Uruguay y Venezuela centrándonos en los productos primarios y las manufacturas basadas en recursos naturales. Distinguimos el comercio con destino a China y el del resto del mundo; para luego estudiar las consecuencias del cambio en el patrón exportador sobre las estructuras económica nacionales. Las principales conclusiones del trabajo serían que, si bien para la mayoría de los países estudiados, se aprecia un proceso de reprimarización de sus exportaciones; solo Brasil y Perú presentan indicios de una reciente dinámica reprimarizadora de su estructura interna, de aún muy corto recorrido.
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Bélanger, Gérard. "Qui paie a bien le droit de choisir." Service social 41, no. 2 (April 12, 2005): 71–85. http://dx.doi.org/10.7202/706570ar.
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Après avoir résumé les décisions gouvernementales prises depuis vingt ans pour limiter la croissance des dépenses pour les soins de santé, le texte rappelle les critiques de différents documents concernant l'excessive centralisation des décisions et les recommandations pour régionaliser le système de santé et de services sociaux et pour encourager la participation. En dernière partie, la question suivante est analysée : est-il réaliste de vouloir décentraliser dans un univers où le financement des unités régionales n'est pas autonome, mais provient totalement de l'autorité centrale ? Le fait demeure : « He who pays the piper calls the tune ».
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Esquivel, Gerardo. "La croissance de l'économie mexicaine sous les gouvernements du PAN et bien au-delà." Problèmes d'Amérique latine 89, no. 2 (2013): 13. http://dx.doi.org/10.3917/pal.089.0013.
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Nguyen, Dinh Tao, Anh Tuan Ho, Ngoc Thao Hoang, Hua Wu, and E. Zhang. "‘Henicorhynchus’ thaitui, a new species of cavefish from Central Vietnam (Teleostei, Cyprinidae)." ZooKeys 965 (September 3, 2020): 85–101. http://dx.doi.org/10.3897/zookeys.965.52751.
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‘Henicorhynchus’thaituisp. nov. is described from a subterranean stream in a karst cave in Phong Nha Ke Bang National Park, Quang Binh Province, Central Vietnam. It differs from all congeners in having a pale pink body in life, smaller eyes with diameter less than the maxillary barbel length, and two pairs of barbels, the maxillary barbel being much longer than the rostral barbel.
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Girolami, Kelli M., SungWoo Kahng, Kellie A. Hilker, and Peter A. Girolami. "Differential reinforcement of high rate behavior to increase the pace of self-feeding." Behavioral Interventions 24, no. 1 (February 2009): 17–22. http://dx.doi.org/10.1002/bin.273.
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Dong, Mai Duc, Phung Van Phach, Nguyen Trung Thanh, Duong Quoc Hung, Pham Quoc Hiep, Nguyen Van Diep, and Renat Shakirov. "ĐẶC ĐIỂM HỆ THỐNG SÔNG CỔ VÀ TÁC ĐỘNG NHÂN SINH DỰA TRÊN KẾT QUẢ MÔ HÌNH TIẾN HÓA TỈ LỆ LỚN KHU VỰC CHÂU THỔ SÔNG HỒNG." Tạp chí Khoa học và Công nghệ biển 19, no. 4 (March 27, 2020): 463–78. http://dx.doi.org/10.15625/1859-3097/19/4/12706.
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The Simclast model has been verified and applied effectively in simulating the delta development for some major deltas in the world. In this study, we applied the model Simclast for simulating the history of the Red river delta development in late Pleistocene-Holocene. Results of the model reveal that the mainland of study area had reduced rapidly during transgression period (10,000-8,000 BP). The morphology changed significantly in the paleo-Red and Day river systems, but slightly in the paleo Thai Binh river system. The paleo-river network had been active in upper part before 11,000 BP and then shifted seaward until 2,000 BP. The river-sea interaction causes erosion and accumulation; as a result the morphology changed remarkably. The paleo-Thai Binh river had been inactive until 5,500 BP and then it was active but the morphology had not varied remarkably. The recent coastline generated from Simclast is relatively in accordance with the present coastline.
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Reddy, Yanala Prashanth Kumar. "Design and Simulation of Electric Treadmill Bike." International Journal for Research in Applied Science and Engineering Technology 9, no. 9 (September 30, 2021): 648–53. http://dx.doi.org/10.22214/ijraset.2021.38003.
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Abstract: Electric treadmill The bicycle is a whole new way of moving. With the help of electricity, walking is no more strenuous than a "walk in the park". The power assistance combined with the equipment increases your walking pace to speed. When you walk on the treadmill bike, push the treadmill back with your feet. The movement of the treadmill gives the signal to the electronic device that drives the motor. The movement of walking. The brake motor slows down the speed. This Paper clearly explains the design and simulation of the electric treadmill bike Keywords: Electric Treadmill Bike, Treadmill Bike, Design, Analysis, Ansys, Electric Bike.
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Briñas Barba, Álvaro. "La reducción de la jornada laboral desde la perspectiva marxista: un análisis de la UE." Papeles de Europa 32, no. 2 (October 2, 2020): 145–61. http://dx.doi.org/10.5209/pade.68028.
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El aumento de la productividad acaecido desde la aparición del sistema capitalista ha permitido que los trabajadores sean capaces de producir la misma cantidad de mercancías en un cada vez menor tiempo necesario. Sin embargo, durante los siglos XX y XXI, este hecho no ha tenido consecuencias sobre el tiempo de trabajo dedicado por la clase asalariada, que, desde finales del siglo XIX, cuando se implantó la jornada laboral de ocho horas, no se ha modificado. El aumento de la productividad, por tanto, podría haber dado lugar a una reducción de la jornada laboral, de tal forma que los trabajadores disfrutaran de un mayor tiempo de ocio. No obstante, esto no ha sucedido debido a que la clase capitalista se ha apropiado de la mayor parte de las ganancias de productividad. En este trabajo, utilizando las bases de la teoría marxista, analizaremos las consecuencias que el aumento de la productividad ha causado sobre la clase capitalista y la clase asalariada, así como los efectos que una reducción de la jornada laboral tendría sobre ambos. Para contrastarlo de forma empírica, se analiza el comportamiento que en este sentido han experimentado cinco economías de la Unión Europea, si bien también se plantean los efectos que una reducción de la jornada laboral tendría en ellas.
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Redfern, Vicky, and Gerri Mortimore. "Right hypochondrial pain leading to diagnosis of cholestatic jaundice and cholecystitis: a review and case study." Gastrointestinal Nursing 17, no. 5 (June 2, 2019): 32–41. http://dx.doi.org/10.12968/gasn.2019.17.5.32.
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The gallbladder stores bile from the liver and releases it into the duodenum. Imbalance in bile components (typically, cholesterol) can lead to cholelithiasis, the crystallisation of choleliths (gallstones). Cholelithiasis is common, affecting a fifth of people in Western countries. The stones can become lodged in the biliary duct and obstruct bile flow. Bile obstruction affects levels of bilirubin, causing cholestatic jaundice. Associated symptoms include nausea, dark urine and pale stools. Gallstones can also cause cholecystitis, the inflammation of the gallbladder. They also often cause pain (biliary colic), especially sudden-onset, episodic, radiating right hypochondrial pain, and biliary pathology is the main cause of upper abdominal pain. Diagnosing these presentations requires a multispectral, holistic assessment comprising numerous investigations, including clinical history, liver function tests, Murphy's sign and abdominal ultrasound. Treatment is usually gallbladder removal surgery (laparoscopic cholecystectomy), with either bile duct exploration or endoscopic retrograde cholangio-pancreatography (ERCP). Good nurse–patient communication is essential to ensure quality of care. The case study presented here covers the assessment and biliary diagnosis of a female patient presenting with severe right hypochondrial pain. The review of existing evidence and the case study should help hepatobiliary nurses deliver quality care for patients presenting with symptoms of gallstones.
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López-Garrido, Javier, Nancy Cheng, Fátima García-Quintanilla, Francisco García-del Portillo, and Josep Casadesús. "Identification of the Salmonella enterica damX Gene Product, an Inner Membrane Protein Involved in Bile Resistance." Journal of Bacteriology 192, no. 3 (November 30, 2009): 893–95. http://dx.doi.org/10.1128/jb.01220-09.
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ABSTRACT The damX gene product of Salmonella enterica serovar Typhimurium is a protein located in the inner membrane. DamX migrates as a 70-kDa protein in SDS-PAGE even though the predicted protein size is 46 kDa. Synthesis of DamX protein occurs in both exponential- and stationary-phase cultures. Disruption of damX causes severe sensitivity to bile. Lack of the outer membrane protein AsmA suppresses bile sensitivity in Salmonella damX mutants.
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Todorov, Vladimir T., Michael Desch, Thomas Schubert, and Armin Kurtz. "The Pal3 Promoter Sequence Is Critical for the Regulation of Human Renin Gene Transcription by Peroxisome Proliferator-Activated Receptor-γ." Endocrinology 149, no. 9 (May 15, 2008): 4647–57. http://dx.doi.org/10.1210/en.2008-0127.
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We recently reported that human renin gene transcription is stimulated by the nuclear receptor peroxisome proliferator-activated receptor (PPAR)-γ in the renin-producing cell line Calu-6. The effect of PPARγ was mapped to two sequences in the renin promoter: a direct repeat hormone response element (HRE), which is related to the classical PPAR response element (PPRE) and a nonconsensus palindromic element with a 3-bp spacer (Pal3). We now find that PPARγ binds to the renin HRE. Neither the human renin HRE nor the consensus PPRE was sufficient to attain the maximal stimulation of renin promoter activity by the PPARγ agonist rosiglitazone. In contrast, the human renin Pal3 element mediates both the full PPARγ-dependent activation of transcription and the PPARγ-driven basal renin gene transcription. The human renin Pal3 sequence was found to selectively bind PPARγ and the retinoid X receptor-α from Calu-6 nuclear extracts. This is in contrast to the consensus PPRE, which can bind other nuclear proteins. PPARγ knockdown paradoxically did not attenuate the stimulation of the endogenous renin gene expression by rosiglitazone. Similarly, a deficiency of PPARγ did not attenuate the activation of the minimal human renin promoter, which contains the endogenous Pal3 motif. However, when the human renin Pal3 site was replaced by the consensus PPRE sequence, PPARγ knockdown abrogated the effect of rosiglitazone on renin promoter activity. Thus, the human renin Pal3 site appears to be critical for the PPARγ-dependent regulation of gene expression by mediating maximal transcription activation, particularly at the low cellular level of PPARγ.
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Shrestha, BR, M. R. Joshi, and T. P. Bohara. "Anaesthetic issues in Brugada electrocardiogram/syndrome undergoing laproscopic common bile duct exploration and laproscopic primary common bile duct closure." Journal of Kathmandu Medical College 2, no. 1 (June 4, 2014): 36–39. http://dx.doi.org/10.3126/jkmc.v2i1.10555.
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Here we report a rare case of Brugada Syndrome in a young Nepalese male undergoing laparoscopic common bile ductexploration and laparoscopic primary common bile duct closure. Recognition of the disease, preparedness to tacklethe developing arrhythmias, avoidance of precipitating drugs, adopting policy of anaesthetic interventions to balancethe autonomic system, maintenance of normothermia and electrolyte balance, avoidance of hypoxia, hypercarbia andacidosis are the key to management of this disease in anaesthetic pursuit.DOI: http://dx.doi.org/10.3126/jkmc.v2i1.10555Journal of Kathmandu Medical College, Vol. 2, No. 1, Issue 3, Jan.-Mar., 2013, Page: 36-39
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Šterbenk, Emil, Marijan Jedovnicki, Štefan Pražnikar, Branko Naveršnik, Vesna Metelko-Skutnik, and Alenka Rošer Drev. "Udejanjanje načel trajnostnega razvoja v malih, antropogeno preobremenjenih porečjih." Dela, no. 28 (December 1, 2007): 323–40. http://dx.doi.org/10.4312/dela.28.323-340.
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Sredi osemdesetih let dvajsetega stoletja so bile vode v Šaleški dolini močno onesnažene. Njihova degradiranost je botrovala nastanku sanacijskega programa.Metodologijo okoljske sanacije porečja Pake je mogoče prilagoditi in uporabiti tudi za druga podobna preobremenjena območja.
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Brucato, FH, and SV Pizzo. "Catabolism of streptokinase and polyethylene glycol-streptokinase: evidence for transport of intact forms through the biliary system in the mouse." Blood 76, no. 1 (July 1, 1990): 73–79. http://dx.doi.org/10.1182/blood.v76.1.73.73.
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Abstract The catabolism of streptokinase (SK) and polyethylene glycol derivatives of SK (PEG-SK) were studied in mice. The clearance and catabolism of SK:plasmin (SK:Pm) and PEG-SK:Pm activator complexes were also investigated. Native 125I-SK cleared rapidly (t1/2 = 15 minutes) from the circulation, with the majority of the ligand accumulating in the liver and gastrointestinal (GI) tract and a substantial fraction also localizing in the kidneys. SK, which was removed from the plasma by the liver, was secreted into bile and then the GI tract. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that 125I-SK recovered from liver and bile was homogeneous and of the same molecular weight (mol wt approximately 50,200) as native SK. PEG-125I-SK cleared slowly (t1/2 greater than 200 minutes), with more than 80% of the preparation localizing in liver and GI tract. The PEG-125I-SK secreted into the bile was also intact. The bile containing 125I-SK was incubated with stoichiometric amounts of plasminogen and electrophoresed under nondenaturing conditions. This study demonstrated that the secreted SK was able to form SK:Pg complexes. SDS-PAGE also showed activation of 125I-Pg that was incubated with recovered bile containing the SK. 125I-SK:Pm catabolism was also studied. In these experiments, the mol wt approximately 42,000 fragment obtained when SK is cleaved by plasmin was found in the bile. This fragment of 125I-SK was not recovered as part of a complex with plasmin, consistent with our previous observations that catabolism of SK:Pm involves transfer of the plasmin to plasma proteinase inhibitors while SK is catabolized independently. By contrast, when PEG-125I-SK:Pm was injected into mice, only intact PEG-125I-SK was found in the bile, consistent with our previous observations that the PEG derivatization blocks its degradation by plasmin.
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Brucato, FH, and SV Pizzo. "Catabolism of streptokinase and polyethylene glycol-streptokinase: evidence for transport of intact forms through the biliary system in the mouse." Blood 76, no. 1 (July 1, 1990): 73–79. http://dx.doi.org/10.1182/blood.v76.1.73.bloodjournal76173.
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The catabolism of streptokinase (SK) and polyethylene glycol derivatives of SK (PEG-SK) were studied in mice. The clearance and catabolism of SK:plasmin (SK:Pm) and PEG-SK:Pm activator complexes were also investigated. Native 125I-SK cleared rapidly (t1/2 = 15 minutes) from the circulation, with the majority of the ligand accumulating in the liver and gastrointestinal (GI) tract and a substantial fraction also localizing in the kidneys. SK, which was removed from the plasma by the liver, was secreted into bile and then the GI tract. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that 125I-SK recovered from liver and bile was homogeneous and of the same molecular weight (mol wt approximately 50,200) as native SK. PEG-125I-SK cleared slowly (t1/2 greater than 200 minutes), with more than 80% of the preparation localizing in liver and GI tract. The PEG-125I-SK secreted into the bile was also intact. The bile containing 125I-SK was incubated with stoichiometric amounts of plasminogen and electrophoresed under nondenaturing conditions. This study demonstrated that the secreted SK was able to form SK:Pg complexes. SDS-PAGE also showed activation of 125I-Pg that was incubated with recovered bile containing the SK. 125I-SK:Pm catabolism was also studied. In these experiments, the mol wt approximately 42,000 fragment obtained when SK is cleaved by plasmin was found in the bile. This fragment of 125I-SK was not recovered as part of a complex with plasmin, consistent with our previous observations that catabolism of SK:Pm involves transfer of the plasmin to plasma proteinase inhibitors while SK is catabolized independently. By contrast, when PEG-125I-SK:Pm was injected into mice, only intact PEG-125I-SK was found in the bile, consistent with our previous observations that the PEG derivatization blocks its degradation by plasmin.
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Athalye-Jape, Gayatri, Megan Nettleton, Ching-Tat Lai, Elizabeth Nathan, Donna Geddes, Karen Simmer, and Sanjay Patole. "Composition of Coloured Gastric Residuals in Extremely Preterm Infants-A Nested Prospective Observational Study." Nutrients 12, no. 9 (August 26, 2020): 2585. http://dx.doi.org/10.3390/nu12092585.
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Green gastric residuals (GR) are often considered as a sign of feed intolerance and discarded in preterm infants. Probiotics are known to enhance feed tolerance in preterm infants. To assess the composition (primary outcome) and volume of discarded green GRs, and feeding outcomes in extremely preterm (EP) infants in a probiotic trial, composition of pale and dark green GRs in the first two weeks of life from EP infants (<28 weeks) in a randomized controlled trial (RCT: SiMPro) of single vs. three-strain probiotics was assessed. Feeding outcomes included time to full feeds (TFF: 150 mL/kg/day) and duration of parenteral nutrition (PN). EP infants given placebo in our previous probiotic RCT served as the reference group. Analysis involved linear regression modelling with clustered standard errors for repeated measurements. GRs of 74/103 from 39 SiMPro infants (18: single-strain, 21: three-strain) were analyzed. Bile acid content was higher but statistically insignificant (825.79 vs. 338.1 µmol/L; p = 0.12) in dark vs. pale green GRs. Mean (95% confidence interval) fat, nitrogen, and carbohydrate loss in GRs over the study period was 0.02 g (0.01–0.03), 0.011 g (0.009–0.013), and 0.05 g (0.04–0.06), respectively. Overall, SiMPro infants had shorter median TFF (10 vs. 14 days, p = 0.02) and duration of PN (10 vs. 16 days, p = 0.022) compared with control group infants. Z scores for growth parameters at discharge were comparable. Discarding dark green GRs meant higher loss of bile acids during early enteral nutrition in EP infants. Probiotic supplementation was associated with reduced TFF and duration of PN.
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De Tommaso, Adriana Maria Alves, Agnes Sumi Kawasaki, and Gabriel Hessel. "Paucity of intrahepatic bile ducts in infancy: experience of a tertiary center." Arquivos de Gastroenterologia 41, no. 3 (September 2004): 190–92. http://dx.doi.org/10.1590/s0004-28032004000300010.
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BACKGROUND: Intrahepatic cholestasis secondary to paucity of bile duct is an alteration of the anatomic integrity of the biliary tract. Can be defined only histologically and, clinically, two categories are recognized: syndromic and non-syndromic, where the prognosis is generally more severe. AIM: To evaluate the history, clinical and biochemical characteristics, etiology and improvement of children who have paucity of intrahepatic bile duct followed at tertiary center. PATIENTS AND METHODS: Eleven children with paucity of intrahepatic bile duct, followed at the Pediatric Hepatology Service of the University Hospital, Campinas, SP, Brazil, were evaluated in the period from 1986 to 2001. RESULTS: Among the patients, three presented the syndromic and eight the non-syndromic form (two with alpha-1-antitrypsin deficiency, one with lues, one secondary to sepsis, three with probable etiology by cytomegalovirus and one without a definite etiology). Referral ranged from 31 to 1185 days. Birth weights ranged from 1920 g to 3590 g. Most of the patients presented pale stools. The median bile duct/portal tract ratio was 0.14. The majority of the children presented a favorable follow-up, regardless of the form of presentation. CONCLUSION: Paucity of intrahepatic bile ducts should be considered in children with cholestasis and its differentiation from extrahepatic causes of neonatal cholestasis is important in order to avoid surgery. Diagnosis of non-syndromic form should not be regarded as unfavorable prognosis, as the evolution is probably related to the etiology in this form of presentation.
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Xu, Bing, Haoyu Lu, Donglei Zhao, Wei Wang, and Hong Ji. "Diversity of macrofungi in Yushan, Jiangsu, China." Mycotaxon 134, no. 3 (October 2, 2019): 581. http://dx.doi.org/10.5248/134.581.
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Mycotaxon is pleased to add a new annotated species distribution list to our 133 previously posted free-access fungae. The 9-page "Diversity of macrofungi in Yushan, Jiangsu, China" by Bing Xu, Haoyu Lu, Donglei Zhao, Wei Wang, and Hong Ji may be downloaded from our website via http://www.mycotaxon.com/mycobiota/index.html
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Jha, Akhilesh Kumar, Rajesh Acharya, Kamal Subedi, Anuj Shrestha, Krishna Rauniyar, and Aurav Bhandari. "Gallbladder Ascariasis : A Rare Case Report." Med Phoenix 3, no. 1 (August 15, 2018): 95–97. http://dx.doi.org/10.3126/medphoenix.v3i1.20770.
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Ascariasis is the most prevalent helminthic infection to infest human beings caused by Ascaris lumbricoides. Rarely the worm migrates through ampulla of Vater and may enter common bile duct. This is a case report of live Ascaris lumbricodes in gallbladder, on USG. Med Phoenix. Vol. 3, Issue. 1, 2018, Page: 95-97
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Eni, Sri Pare, Margareta M. Sudarwani, and Galuh Widati. "Pemanfaatan Material Daur Ulang Untuk Pengembangan Karya Seni Dan Kerajinan Di Kelurahan Kebon Pala Kecamatan Makasar Jakarta Tim." JURNAL Comunità Servizio : Jurnal Terkait Kegiatan Pengabdian kepada Masyarakat, terkhusus bidang Teknologi, Kewirausahaan dan Sosial Kemasyarakatan 2, no. 1 (June 2, 2020): 301–11. http://dx.doi.org/10.33541/cs.v2i1.1510.
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Program pengabdian pada masyarakat dilaksanakan dalam berbagai bentuk dalam rangka pendampingan pembinaan kampung hijau. Pendampingan pembinaan kampung hijau di kelurahan Kebon Pala Jakarta Timur juga telah dilaksanakan sejak tahun 2015. Masyarakat di kelurahan Kebon Pala sudah lebih peduli terhadap kebersihan lingkungan nya sehingga pendampingan berupa penyuluhan dan pelatihan yang dilakukan di Kebon Pala bisa langsung dirasakan manfaatnya. Terbukti dengan pelatihan membuat kreativitas bambu untuk penghijauan, lukisan untuk penghijauan dapat segera dilaksanakan oleh masyarakat. Jenis pengabdian pada masyarakat yang akan dibahas di sini termasuk dalam kategori “pemberdayaan masyarakat” melalui pelatihan kreativitas membuat kerajinan dari bahan / material daur ulang sampah dan atau pemanfaatan material alami lokal menjadi sesuatu barang yang berguna, berharga dan mempunyai unsur seni. Dalam pembinaan kampung hijau di Kelurahan Kebon Pala, di bina juga unsur kemandirian masyarakat melalui pemberdayaan ibu ibu dalam berkreasi dengan bahan daur ulang, serta mengembangkan usaha kecil seperti budi daya tanaman Lidah buaya dan tanaman obat untuk jamu dan berbagai makanan kecil. Generasi muda, karang taruna dan anak anak di kelurahan Kebon Pala sangat aktif dengan berbagai kegiatan, oleh sebab itu sangat positip jika sedari kecil anak anak sudah diperkenalkan budaya cinta lingkungan, pengelolaan limbah lingkungan dengan baik bahkan memanfaatkannya menjadi benda bernilailainnya.
Kata Kunci: karya seni dan kerajinan, Kebon Pala, material daur ulang, pemanfaatan
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Bucur, Pálma, Ibolya Fülöp, and Md Croitoru. "Romanian young people’s drinking habits." Acta Medica Marisiensis 60, no. 5 (October 1, 2014): 186–90. http://dx.doi.org/10.2478/amma-2014-0039.
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Abstract Objective: Alcohol use can cause social problems. Beside alcoholism the “binge drinking” and the “pre-drinking” could be a harmful form of alcohol use based on scientific literature data. In this study the alcohol use behaviors and associated problems were evaluated among young people aged between 14-30 years. Methods: The study was carried out using a self reporting questionnaire, containing the Alcohol Use Disorders Identification Test (AUDIT) developed by World Health Organization and 10 more questions. This questionnaire was created in two languages (Romanian, Hungarian) and uploaded to the on-line survey page (www.kwiksurveys.com). The link was shared at different Facebook groups. Results: The questionnaire was completed by 933 young people. There was a predominance of females (66.99%) and the participant’s average age was 22.12 years (±2.78). The results show that males’ relative risk (RR) for alcoholism is 7.18 (CI95%, 3.47-14.85). Majority (539; 57.77%) of the participants had at least one time binge drinking, from this 36.66% were occasionally binge drinkers and 21.11% were regular binge drinkers. More males binge drinkers were found then females (RR: 1.72; CI95%, 1.55-1.90). The people who participate in pre-drinking have RR of 2.14 (CI95%, 1.89-2.41) for binge drinking. The binge drinkers have RR of 2.58 (CI95%, 1.78-3.37) for drunk driving. Conclusions: Binge drinking is a really common habit among young people. Measures to reduce the risk or prevent the harmful consequences of this habit should be employed.
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Sippel, C. J., M. Ananthanarayanan, and F. J. Suchy. "Isolation and characterization of the canalicular membrane bile acid transport protein of rat liver." American Journal of Physiology-Gastrointestinal and Liver Physiology 258, no. 5 (May 1, 1990): G728—G737. http://dx.doi.org/10.1152/ajpgi.1990.258.5.g728.
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The aim of this study was to isolate the Na(+)-independent bile acid transporter from rat canalicular plasma membranes by affinity chromatography. The affinity matrix used consisted of lysylcholic acid covalently linked to CH-Sepharose 4B, resulting in an anionic ligand essentially identical to glycocholic acid. The protein fraction, adsorbed and eluted from the affinity column, was markedly enriched in a 100-kDa band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) compared with the total membrane and membrane extract. The 100-kDa band, further purified by preparative SDS-PAGE, was electroeluted from excised gel fragments and used as an immunogen for antibody production in rabbits. The immune serum, but not preimmune serum, specifically recognized a single, 100-kDa polypeptide on one- and two-dimensional immunoblots of canalicular membranes. In contrast, no reactivity was observed with proteins in liver basolateral or ileal brush-border membranes. The 125I-labeled protein was immunoprecipitated from membrane extracts solubilized in NP-40 and was found to migrate with a pI of 5.3 on two-dimensional electrophoresis. The apparent molecular weight of the protein was reduced by 50% after deglycosylation with N-glycanase. The 100-kDa protein was localized specifically and exclusively by immunocytochemical methods to the bile canalicular domain of the hepatocyte plasma membrane. Moreover, the immunoglobin G fraction prepared from the antiserum significantly inhibited taurocholate transport by canalicular membrane vesicles and decreased the covalent labeling of the 100-kDa protein by the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. Thus the isolation of a single 100-kDa protein by bile acid-affinity chromatography, as well as the inhibitory effects of antibodies directed against this polypeptide, provide further support for its role in the canalicular transport of bile acids.
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OKUNO, Masaaki, Emi ARIMOTO, Yukiko IKENOBU, Tsutomu NISHIHARA, and Masayoshi IMAGAWA. "Dual DNA-binding specificity of peroxisome-proliferator-activated receptor γ controlled by heterodimer formation with retinoid X receptor α." Biochemical Journal 353, no. 2 (January 8, 2001): 193–98. http://dx.doi.org/10.1042/bj3530193.
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The peroxisome-proliferator-activated receptor γ (PPARγ) is a member of the steroid/thyroid nuclear receptor superfamily of ligand-activated transcription factors. PPARγ forms a heterodimer with the retinoid X receptor α (RXRα) and binds to a common consensus response element consisting of a direct repeat of two hexanucleotides spaced by one nucleotide (DR1 motif). However, other hexamer configurations for binding of PPARγ have not been considered. By using PCR-mediated random site selection, the DNA sequence preferences for PPARγ binding were examined. In this study, we have demonstrated that PPARγ has dual DNA-binding specificity; binding to both the DR1 motif and a palindromic sequence with three bases as spacers (Pal3 motif). The consensus sequence selected by equimolar amounts of PPARγ and RXRα was a perfect DR1 motif, whereas a relatively large population of Pal3 was observed when a 30-fold molar excess of PPARγ over RXRα was used. Gel-shift analysis revealed that the PPARγ homodimer could bind to Pal3 and that the affinity constant of the PPARγ homodimer for Pal3 was nearly the same as that of the PPARγ/RXRα heterodimer for DR1. The addition of RXRα decreased the binding affinity of PPARγ for Pal3, indicating that the DNA-binding specificity of PPARγ could be altered by heterodimer formation with RXRα.
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Koirala, Ashok, Dipendra Thakur, Sunit Agrawal, and Abhilasha Sharma. "Bacteriological analysis of bile in laparoscopic cholecystectomy patients." Journal of Nobel Medical College 7, no. 1 (August 22, 2018): 41–44. http://dx.doi.org/10.3126/jonmc.v7i1.20846.
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Background: Laparoscopic cholecystectomy is commonly performed operation for symptomatic gall stone disease. The presence of stones within the biliary system is associated with the bacterial colonization of the bile. The aim of this study is to evaluate the bacteriological profile of the bile and to determine appropriate antibiotics for preoperative prophylaxis in laparoscopic cholecystectomy patients. Material & Methods: A prospective study was carried out in NMCTH, Biratnagar from June2017- May 2018. A total of 100 patients admitted through OPD of our hospital for laparoscopic cholecystectomy were studied. About 5ml of bile aspirated from gall bladder was transported to laboratory in sterile syringe for culture and sensitivity. All age groups and both sex were included. Results: Bile culture was positive in 16 patients. The most common organisms isolated from bile was Escherichia coli (50%) followed by Klebsiella species (25%). Histopathological report of all 16 cases revealed chronic cholecystitis. Wound infection was seen in 5% cases and all were bile culture positive. Most sensitive drug was found to be aminoglycoside group followed by piperacilin and tazobactam. Conclusion: Most common organism isolated from bile culture was Escherichia coli. Aminoglycoside group of drugs was found to be more promising compared to other group of drugs. It can be considered as a first line drug for preoperative prophylaxis for patients undergoing laparoscopic cholecystectomy for symptomatic cholelithiasis. Journal of Nobel Medical College Volume 7, Number 1, Issue 12, January-June 2018, Page: 41-44
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Kirk, Cheryl M., David Lewin, and John Lazarchick. "Primary Hepatic B-Cell Lymphoma of Mucosa-Associated Lymphoid Tissue." Archives of Pathology & Laboratory Medicine 123, no. 8 (August 1, 1999): 716–19. http://dx.doi.org/10.5858/1999-123-0716-phbclo.
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Abstract Mucosa-associated lymphoid tissue (MALT) lymphomas are low-grade B-cell lymphomas that occur in a variety of extranodal sites but rarely as a primary hepatic lymphoma. We describe the histological findings, immunophenotype, and immunohistochemistry of one such lymphoma found incidentally in a 69-year-old woman. The lymphoid infiltrate invaded the liver in a serpiginous configuration with entrapment of nodules of normal liver. Reactive follicles were surrounded by intermediate-sized lymphoid cells with slightly irregular nuclei and pale cytoplasm. Only a few scattered lymphoepithelial lesions were identified since most of the bile ducts were destroyed. The immunophenotype determined by flow cytometry identified the lymphoid cells as being CD19, CD20 positive and exhibiting λ light chain restriction. CD5, CD10, and CD23 were negative. Immunohistochemistry showed the neoplastic cells to be positive for CD20 (L-26) and bcl-2. The reactive follicles were negative for bcl-2. CD3 showed only a few scattered T cells. Cyclin D1 did not stain the neoplastic cells. Cytokeratin (AE1/AE3) highlighted the lymphoepithelial lesions and residual bile ducts. MALT lymphomas need to be recognized and distinguished from other B-cell lymphomas, particularly mantle cell lymphomas, because of the difference in behavior and treatment.
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ZAVAGLIA, ANDREA GÓMEZ, GUILLERMO KOCIUBINSKI, PABLO PÉREZ, and GRACIELA DE ANTONI. "Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation." Journal of Food Protection 61, no. 7 (July 1, 1998): 865–73. http://dx.doi.org/10.4315/0362-028x-61.7.865.
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Twenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized numerical analysis enabled strains to be grouped in two main clusters. Strains of Bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 similarity. The predominant species among isolated strains from infant feces were B. bifidum, B. longum, and B. breve. Probiotic and technological indicators such as surface properties, inhibitory capacity, resistance to bile and low pH, and ability to grow under aerobic conditions were studied. Not all desirable characteristics were present in a single strain. In general, adherent and inhibitory strains were not resistant to bile, low pH, and aerobic conditions. Only 10 of 40 strains were resistant to 0.5% bile.
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Bukhor, Saiful i. "Analisis Perbandingan Fitur Search Engine." INFORMAL: Informatics Journal 3, no. 1 (February 25, 2019): 17. http://dx.doi.org/10.19184/isj.v3i1.9850.
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Search engines are used in the web as a tool for information retrieval. Web Server is a large warehouse of heterogeneous and unstructured data so that to filter out relevant information from people, a search engine is needed. Search engines usually consist of page repositories, indexing modules, query modules and ranking modules. Search engines do not work alone, besides that there is a web browser that supports the work of this search engine to be more optimal. A browser is software that is run on a user's computer (user) that displays web documents or information taken from a web server [1]. A browser is the type of intermediary the user uses most often. This paper aims to analyze three search engines namely Google, Yahoo, and Bing based on existing features. These features include web search, image search, video search, news search, route search, book search, change search settings, display number of views, shopping, language translator. Google stands as the best search engine among all search engines, which works using the Page Rank algorithm. Page Rank is a numerical value that determines the importance of a web page by calculating the number of backlinks.
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Knotek, Zdeněk, Gerry M. Dorrestein, Anna Hrdá, Aleš Tomek, Pavel Proks, Zora Knotková, Vladimír Jekl, and William Lewis. "Hepatocellular carcinoma in a green iguana – a case study." Acta Veterinaria Brno 80, no. 3 (2011): 243–47. http://dx.doi.org/10.2754/avb201180030243.
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A 3.10 kg, six-year old male green iguana (Iguana iguana) suffered several weeks of decreased activity, loss of body weight, anorexia and change of skin colour. Physical examination revealed a cachectic depressed lizard, with marked pale mucous membranes. The complete blood count revealed a leukocytosis (46.5 × 109/l), elevated heterophils (23.25 × 109/l) and azurophils (23.25 × 109/l), decreased haemoglobin concentration (29.00 g/l), low haematocrit (0.16 l/l) and erythropenia (0.35 × 1012/l). Abnormalities of the plasma chemistry panel included increased activity of aspartate aminotransferase (6.05 μkat/l) and creatinephosphokinase (217.91 μkat/l), increased concentrations of uric acid (321.58 μmol/l), phosphorus (4.04 mmol/l) and bile acids (120.21 μmol/l), as well as a decreased concentration of glucose (3.72 mmol/l), cholesterol (0.637 mmol/l) and triglycerides (0.09 mmol/l). A lateral radiograph revealed soft-tissue opacity present in the region of the liver. Magnetic resonance imaging showed a markedly enlarged left liver lobe distended caudally and displacing the other abdominal organs. The signal intensity of the liver parenchyma was slightly hyperintense, the ventro-medial part of the left lobe showed a hypointense signal. Endoscopic examination of the coelomic cavity revealed a greatly enlarged pale liver and the presence of a straw-coloured modified transudate. Based on histopathological examination of the liver hepatocellular carcinoma was diagnosed. Despite supportive treatment the patient died on the sixth day of hospitalisation.
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Svetlov, Stanislav I., Katherine M. Howard, Michael S. Debuysere, and Merle S. Olson. "Secretory PAF-acetylhydrolase of the rat hepatobiliary system: characterization and partial purification." American Journal of Physiology-Gastrointestinal and Liver Physiology 274, no. 5 (May 1, 1998): G891—G900. http://dx.doi.org/10.1152/ajpgi.1998.274.5.g891.
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Hepatocytes and Kupffer cells in primary culture both secrete plasma-type platelet-activating factor-acetylhydrolase (pPAF-AH) into serum-free culture medium. The rate of secretion of pPAF-AH by Kupffer cells was 20 to 25 times higher than from hepatocytes, and Kupffer cells expressed a higher level of pPAF-AH mRNA than did hepatocytes. Purified liver cell-secreted pPAF-AH exhibited a major protein band of 65–67 kDa on SDS-PAGE; this was the band predominantly labeled when the enzyme catalytic center was reacted with [3H]diisopropylfluorophosphate ([3H]DFP). Rat bile collected from cannulated bile ducts contained significant PAF-AH activity, and bile samples possessed a prominent band at 30–32 kDa, which was the exclusive target for [3H]DFP. Experiments using tunicamycin, an inhibitor of N-linked glycosylation, and endoglycosidase H suggested that pPAF-AH secreted constitutively by cultured hepatocytes and Kupffer cells is glycosylated. The present study supports the notion that hepatic secretion of pPAF-AH into the blood contributes to the regulation of PAF and oxidized phospholipid levels in the circulation, whereas secretion of PAF-AH into the bile may allow hepatic control of these phospholipid signaling molecules in the gastrointestinal tract.
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Tamura, Glen S., and Aphakorn Nittayajarn. "Group B Streptococci and Other Gram-Positive Cocci Bind to Cytokeratin 8." Infection and Immunity 68, no. 4 (April 1, 2000): 2129–34. http://dx.doi.org/10.1128/iai.68.4.2129-2134.2000.
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ABSTRACT Group B streptococci (GBS) adhere to surface receptors present on epithelial cells; these receptors include fibronectin and laminin. To identify other possible receptors, plasma membranes from A549 cells, a respiratory tract epithelial cell line, were prepared. These plasma membranes were tested in a protein blot analysis using radiolabeled GBS as a probe. GBS adhered to two species, with molecular masses of 50 kDa (p50) and 57 kDa (p57). We concluded that p50 and p57 correspond to two forms of cytokeratin 8 (CK8) on the basis of the following results: (i) protein blot results demonstrated that p50 and p57 exactly comigrated with two forms of CK8 after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE); (ii) p50 and p57 exactly comigrated with CK8 after separation by two-dimensional PAGE; (iii) CK8 in solution bound to GBS, as demonstrated by immunoblot analysis of proteins from A549 lysates that bound to GBS in a liquid-phase assay; and (iv) radiolabeled GBS bound to A549 lysate-derived CK8 that had been captured in anti-CK8-coated microtiter wells. CK8 bound to COH1-13, an acapsular mutant of COH1, demonstrating that adherence is not mediated by capsular polysaccharide. Trypsin-treated GBS did not bind to CK8, indicating that adherence is mediated via a protein on the surface of GBS. Soluble CK8 bound to six of six GBS strains tested. Soluble CK8 also bound toStaphylococcus aureus, Lactococcus lactis,Enterococcus faecalis, and Streptococcus pyogenes. We hypothesize that adherence of GBS to cytokeratin may be important for maintenance of colonization at sites of keratinized epithelium, such as the vagina, or for adherence of these bacteria to damaged epithelial cells at other sites.
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Ray, Chad A., Linda E. Gfell, Tiffany L. Buller, and Richard L. Gregory. "Interactions of Streptococcus mutansFimbria-Associated Surface Proteins with Salivary Components." Clinical Diagnostic Laboratory Immunology 6, no. 3 (May 1, 1999): 400–404. http://dx.doi.org/10.1128/cdli.6.3.400-404.1999.
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ABSTRACT Streptococcus mutans has been implicated as the major causative agent of human dental caries. S. mutans binds to saliva-coated tooth surfaces, and previous studies suggested that fimbriae may play a role in the initial bacterial adherence to salivary components. The objectives of this study were to establish the ability of an S. mutans fimbria preparation to bind to saliva-coated surfaces and determine the specific salivary components that facilitate binding with fimbriae. Enzyme-linked immunosorbent assay (ELISA) established that the S. mutans fimbria preparation bound to components of whole saliva. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot techniques were used to separate components of whole saliva and determine fimbria binding. SDS-PAGE separated 15 major protein bands from saliva samples, and Western blot analysis indicated significant binding of the S. mutans fimbria preparation to a 52-kDa salivary protein. The major fimbria-binding salivary protein was isolated by preparative electrophoresis. The ability of the S. mutans fimbria preparation to bind to the purified salivary protein was confirmed by Western blot analysis and ELISA. Incubation of the purified salivary protein with the S. mutans fimbria preparation significantly neutralized binding of the salivary protein-fimbria complex to saliva-coated surfaces. The salivary protein, whole saliva, and commercial amylase reacted similarly with antiamylase antibody in immunoblots. A purified 65-kDa fimbrial protein was demonstrated to bind to both saliva and amylase. These data indicated that the S. mutans fimbria preparation and a purified fimbrial protein bound to whole-saliva-coated surfaces and that amylase is the major salivary component involved in the binding.
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Takeuchi, Tetsuo, Kenji Niiya, Ichiro Kubonishi, and Isao Miyoshi. "A Plasminogen Activator Inhibitor-2 from a Promyelocytic Leukemia Cell Line, PL-21, Binds to the Carboxy-Terminal Chain of Plasminogen Activators." Thrombosis and Haemostasis 63, no. 02 (1990): 259–64. http://dx.doi.org/10.1055/s-0038-1645205.
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SummaryA promyelocytic leukemia cell line, PL-21, was found to produce an inhibitor of plasminogen activators (PAI). The PAI reacted to anti-PAl-2 but not anti-PAI-1 anti-serum and had an apparent molecular weight of 43 kDa on unreduced SDS-PAGE. The PAI inhibited not only urokinase-type plasminogen activator (u-PA) but single- and two-chain tissue-type plasminogen activators (t-PAs) on plasminogen-containing fibrin plate. It formed SDS-stable complexes with both t-PA and u-PA but not with prourokinase as demonstrated by both fibrin zymography and immunoblotting using anti-PA and anti-PAI-2 antisera after SDS-PAGE. These complexes were still present even after reduction with dithiothreitol. The PAI appears to bind to the carboxy-terminal chain of both PAs, because the part of the band corresponding to the carboxy-terminal chain of PAs moved to an upper position as a result of complex formation when two-chain form of PAs were incubated with the PAI and analyzed by SDS-PAGE followed by immunoblotting
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Phillips, DJ, MT Hearn, and DM de Kretser. "Inhibin, activin and follistatin bind preferentially to the transformed species of alpha 2-macroglobulin." Journal of Endocrinology 155, no. 1 (October 1, 1997): 65–71. http://dx.doi.org/10.1677/joe.0.1550065.
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alpha 2-Macroglobulin (alpha 2-M), a major serum glycoprotein, has been implicated as a low-affinity binding protein for inhibin and activin. In serum, alpha 2-M exists as two major species, a native form that is abundant and stable, and a transformed ('fast') species that is rapidly cleared from the circulation via alpha 2-M receptors. In this study inhibin, activin and their major binding protein follistatin were investigated for their ability to bind to the native or transformed species of purified human alpha 2-M. Using native PAGE and size exclusion chromatography, radiolabelled inhibin, activin and follistatin bound to the transformed alpha 2-M. Inhibin and follistatin did not bind significantly to native alpha 2-M, whereas activin was able to bind to the native species but with a lower capacity compared with that to transformed alpha 2-M. Under reducing conditions, binding of these hormones to alpha 2-M was abolished. These findings implicate alpha 2-M as a mechanism whereby inhibin, activin and follistatin may be removed from the circulation through alpha 2-M receptors, but also whereby activin can be maintained in the circulation through its ability to bind to native alpha 2-M.
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Font-Julian, Cristina I., José-Antonio Ontalba-Ruipérez, and Enrique Orduña-Malea. "Hit count estimate variability for website-specific queries in search engines." Aslib Journal of Information Management 70, no. 2 (March 19, 2018): 192–213. http://dx.doi.org/10.1108/ajim-10-2017-0226.
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Purpose The purpose of this paper is to determine the effect of the chosen search engine results page (SERP) on the website-specific hit count estimation indicator. Design/methodology/approach A sample of 100 Spanish rare disease association websites is analysed, obtaining the website-specific hit count estimation for the first and last SERPs in two search engines (Google and Bing) at two different periods in time (2016 and 2017). Findings It has been empirically demonstrated that there are differences between the number of hits returned on the first and last SERP in both Google and Bing. These differences are significant when they exceed a threshold value on the first SERP. Research limitations/implications Future studies considering other samples, more SERPs and generating different queries other than website page count (<site>) would be desirable to draw more general conclusions on the nature of quantitative data provided by general search engines. Practical implications Selecting a wrong SERP to calculate some metrics (in this case, website-specific hit count estimation) might provide misleading results, comparisons and performance rankings. The empirical data suggest that the first SERP captures the differences between websites better because it has a greater discriminating power and is more appropriate for webometric longitudinal studies. Social implications The findings allow improving future quantitative webometric analyses based on website-specific hit count estimation metrics in general search engines. Originality/value The website-specific hit count estimation variability between SERPs has been empirically analysed, considering two different search engines (Google and Bing), a set of 100 websites focussed on a similar market (Spanish rare diseases associations), and two annual samples, making this study the most exhaustive on this issue to date.
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Reason, D. C. "A murine hybridoma with large cytoplasmic inclusions of kappa light chains." Journal of Experimental Medicine 165, no. 2 (February 1, 1987): 578–83. http://dx.doi.org/10.1084/jem.165.2.578.
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A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.
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Larrea Melgarejo, Felipe. "HUME POR DELEUZE. EL EMPIRISMO COMO GÉNESIS DE LA SUBJETIVIDAD." Síntesis. Revista de Filosofía 1, no. 1 (October 5, 2018): 23. http://dx.doi.org/10.15691/0718-5448vol1iss1a233.
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<div class="page" title="Page 1"><div class="layoutArea"><div class="column"><div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p>En 1953 Deleuze publica su primer libro, llamado Empirisme et subjetivité. Essai sur la nature humain selon Hume. Deleuze señala en primer lugar una afirmación distinta con respecto al empirismo de Hume, es decir, señalar que el empirismo es definible a partir de una relación intrínseca con los sentidos, y que el conocimiento, en primera instancia, proviene de ellos. Lo que advertirá Deleuze es que el empirismo debe ser definido, antes que todo, en relación con el problema de la subjetividad. El empirismo constituirá una doctrina que plantea que la subjetividad no está dada, sino que, más bien, debe ser producida o constituida (a esto le llamaremos una génesis de la subjetividad). ¿De qué manera se produce la génesis? A través de la acción de ciertos principios que son extensivos a la naturaleza humana: la pasión y la asociación. El Hume de Deleuze versará sobre dicho movimiento, y le permitirá al autor francés elaborar un primer índice de su metafísica, o lo que llamará, unos años después, empirismo trascendental.</p></div></div></div></div></div></div>
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Corbí Sáez, María I. "La relación literaria de Ramón Gómez de la Serna y de Valery Larbaud. Contribución a un nuevo acercamiento según los epistolarios de algunos de los «Potassons»." Çédille 7 (April 1, 2011): 75. http://dx.doi.org/10.21071/ced.v7i.5456.
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Il est fort bien connu que Ramón Gómez de la Serna a atteint sa diffusion internationale grâce aux efforts de son premier critique et traducteur français. Valery Larbaud, avec un grand enthousiasme et un grand dévouement, aidé par sa vision européiste et internationale de la littérature, guidé par une perspicacité sans égale, sut alors dénicher dans les Greguerías une qualité littéraire et des aspects novateurs qu’il voulut partager avec ses amis écrivains et intellectuels, de même qu’avec un lectorat français, impulsant donc en France une campagne publicitaire et de diffusion de l’œuvre de l’écrivain madrilène. Voici une page de la littérature comparée qui a été parfois interprétée de façon partielle ou même erronée, puisque bien souvent on n’a bien voulu voir que le froid intérêt de Ramón Gómez de la Serna envers son mécène français. Du fait que la correspondance de Valery Larbaud à l’amphytrion du Pombo a été égarée et que l’on ne dispose que des lettres de l’auteur espagnol, notre article prétend contribuer à offrir de nouvelles leurs sur la relation littéraire de ces deux hommes à partir de la correspondance de certains « Potassons »…
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Pischon, Hannah, Anne Petrick, Matthias Müller, Nils Köster, Jörg Pietsch, and Lars Mundhenk. "Grayanotoxin I Intoxication in Pet Pigs." Veterinary Pathology 55, no. 6 (August 2, 2018): 896–99. http://dx.doi.org/10.1177/0300985818789482.
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Contaminated honey is a common cause of grayanotoxin intoxication in humans. Intoxication of animals, especially cattle, is usually due to ingestion of plants of the Ericaceae family, such as Rhododendron. Here, we report the ingestion of Pieris japonica as the cause of grayanotoxin I intoxication in 2 miniature pigs that were kept as pets. The pigs showed sudden onset of pale oral mucosa, tachycardia, tachypnea, hypersalivation, tremor, and ataxia that progressed to lateral recumbency. The pathological examination of one pig revealed no specific indications for intoxication except for the finding of plant material of Pieris japonica in the intestine. Grayanotoxin I was identified in the ingested plant, gastric content, blood, liver, bile, kidney, urine, lung, and skeletal muscle via HPLC-MS/MS. Grayanotoxin I should be considered as a differential etiological diagnosis in pigs with unspecific signs and discovery of ingested plant material as the only indication in the pathologic examination.
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TAMS, Jeppe W., Anders H. JOHNSEN, and Jan FAHRENKRUG. "Identification of pituitary adenylate cyclase-activating polypeptide1-38-binding factor in human plasma, as ceruloplasmin." Biochemical Journal 341, no. 2 (July 8, 1999): 271–76. http://dx.doi.org/10.1042/bj3410271.
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125I-Pituitary adenylate cyclase-activating polypeptide (PACAP) 1-38 is able to bind a factor in human plasma, which can be displaced by unlabelled PACAP 1-38 and PACAP 28-38 but not by the other biologically active form, PACAP 1-27. Likewise, 125I-PACAP 28-38 binds this plasma factor, whereas 125I-PACAP 1-27 does not. Apparent Kd values were measured to be 12.0±1.3 and 3.4±1.5 nM for PACAP 1-38 and PACAP 28-38, respectively, using a competition assay with 125I-PACAP 28-38. Purification of the PACAP 1-38-binding factor from human blood was made by ethanol precipitation of serum followed by Ni2+-chelating and anion-exchange chromatography. A 120-kDa band on SDS/PAGE, as well as some proteolytic products, was blotted on to PVDF membrane and their N-terminal amino acid sequences determined. In combination with a mass-spectrometric fingerprinting of a tryptic digest of the 120-kDa band, this PACAP 1-38-binding factor was identified as ceruloplasmin. Purified commercial ceruloplasmin shows identical mobility on SDS/PAGE to the PACAP 1-38-binding factor and the same binding characteristics to PACAP 1-38, 1-27 and 28-38, using the same amount of ceruloplasmin as was expected to be found in the human plasma. Furthermore, the ability of plasma to bind 125I-PACAP 1-38 or 28-38 disappeared when ceruloplasmin was immunoprecipitated from plasma with rabbit anti-human ceruloplasmin Ig.
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Aprianto, Dian, Christine Wulandari, and Niskan Walid Masruri. "Karbon Tersimpan Pada Kawasan Sistem Agroforestry Di Register 39 Datar Setuju Kphl Batutegi Kabupaten Tanggamus." Jurnal Sylva Lestari 4, no. 1 (April 7, 2016): 21. http://dx.doi.org/10.23960/jsl1421-30.
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Datar Setuju Resort is the one of six resort located in Register 39 KPHL Batutegi. This area is managed by implementing agroforestry system Research aims to determine amount of stock carbon applied by publics in Register 39 Datar Setuju, held on March 2015 at Register 39 Datar Setuju KPHL Batutegi Tanggamus Regency. Beside to knowing amount of the stock carbon, this research also conducted to determine the dominant vegetation. Reseachers used Summed Dominance Ratio (SDR) and Importance Value Index (IVI) methods to determine the most dominant vegetations and used allometric equation to find out amount biomass and necromass of trees. Research results show that the most dominant vegetation on tree phase was cemara (Casuarina junghuniana) IVI large was 109,91%, for pale phase was dadap (Erythrina sp.) IVI large was 300%, for stake phase was kopi (Coffea arabica) IVI large was 300%, and for seedling phase was rumput teki (Cyperus rotundus) IVI large was 200%. Stock carbons at agroforestry system area Register 39 Datar Setuju KPHL Batutegi on HKm Sinar Harapan amount of 123,33 ton/ha, on HKm Bina Wana Jaya 1 amount of 265,20 ton/ha, and of HKm Bina Wana Jaya 2 amount of 146,20 ton/ha, average of stock carbon value at Register 39 Datar Setuju amount of 178,24 ton/ha. Key words: stock carbon, agroforestry
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Tarte, Kendall B. "Prefatory Strategies in La Puce de Madame des-Roches: From the Salon to the Page." Renaissance and Reformation 40, no. 1 (January 1, 2004): 35–45. http://dx.doi.org/10.33137/rr.v40i1.8944.
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Oeuvre collective des habitués de Madeleine et Catherine Des Roches lors des Grands Jours de Poitiers en 1579, l'anthologie La Puce de Madame des-Roches (1582) illustre la dynamique qui caractérise leur salon. Cet article propose une considération du livre imprimé, et en particulier des paratextes qui éclairent les stratégies textuelles et éditoriales. Ces paratextes — tels la page de titre, une préface du compilateur Jacques de Sourdrai, une deuxième préface qui remanie une lettre d’Estienne Pasquier — montrent la collaboration et l’échange qui ont contribué à la composition aussi bien qu'à la publication de La Puce.
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Faioni, EM, CT Esmon, NL Esmon, and PM Mannucci. "Isolation of an abnormal protein C molecule from the plasma of a patient with thrombotic diathesis." Blood 71, no. 4 (April 1, 1988): 940–46. http://dx.doi.org/10.1182/blood.v71.4.940.bloodjournal714940.
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Protein C has been purified from the plasma of a patient with thrombotic diathesis. Both before and after isolation, the protein showed reduced capacity to hydrolyze synthetic substrates and to anticoagulate plasma. Proteolysis with the soluble thrombin- thrombomodulin complex proceeded normally and to completion as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Approximately one-third of the protein is functional, indicating a heterozygous defect. Indirect studies suggest that the abnormal component can bind to protein S and phospholipids. Both forms of activated protein C can also incorporate radiolabeled diisopropylfluorophosphate.
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Abbiss, Chris R., Megan L. R. Ross, Laura A. Garvican, Neil Ross, Torben Pottgiesser, John Gregory, and David T. Martin. "The distribution of pace adopted by cyclists during a cross-country mountain bike World Championships." Journal of Sports Sciences 31, no. 7 (April 2013): 787–94. http://dx.doi.org/10.1080/02640414.2012.751118.
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Bresciani, Alberto, Ottavia Cecchetti, Antonino Missineo, Pier Giorgio Pacifici, Licia Tomei, and Steven Rodems. "Development of a Broadly Applicable Assay for Measurement of Glycan-Directed Enzymatic Activity." SLAS DISCOVERY: Advancing the Science of Drug Discovery 23, no. 9 (June 22, 2018): 941–50. http://dx.doi.org/10.1177/2472555218782625.
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Glycosylation is a key posttranslational modification that tags protein to membranes, organelles, secretory pathways, and degradation. Aberrant protein glycosylation is present both in acquired diseases, such as cancer and neurodegeneration, and in congenital disorders of glycosylation (CDGs). Consequently, the ability to interrogate the activity of enzymes that can modify protein glycan moieties is key for drug discovery projects aimed at finding modulators of these enzymes. To date, low-throughput technologies such as SDS-PAGE and mass spectrometry have been used, which are not suitable for compound screening in drug discovery. In the present work, a broadly applicable time-resolved fluorescence resonance energy transfer (TR-FRET) assay was developed that can determine the activity of endoglycosidase enzymes in high-throughput formats. The assay was validated using PNGaseF and EndoH as tool deglycosylases. Even though the current setup is based on the recognition of glycans that bind concanavalin A (ConA), the assay concept can be adapted to glycans that bind other lectins.
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Bumbudsanpharoke, Nattinee, Jungwook Choi, Insik Park, and Seonghyuk Ko. "Facile Biosynthesis and Antioxidant Property of Nanogold-Cellulose Fiber Composite." Journal of Nanomaterials 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/146460.
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Direct synthesis of gold nanoparticles (AuNPs) on cellulose fiber has been successfully performed via facile green approach using lignin-containing unbleached kraft softwood pulp. The resulting AuNPs composited fibers showed apparent color change from pale yellow to purplish-dark brown by varying the amount of gold ions (Au3+) due to the surface plasmon resonance of nanogold. Further confirmation of AuNP formation on the fiber surface was conducted by UV-Vis diffuse reflectance spectroscopy (DRS), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM). X-ray photoelectron spectroscopy (XPS) analysis revealed that gold nanoparticles formed on the fiber are well-defined pure metallic gold, indicating that Au3+ions are efficiently bioreduced into Au0and bind to the fiber surface. Antioxidant activity was evaluated by decomposition of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) in dark and light condition. As-prepared unbleached kraft fiber-AuNP composite showed significantly enhanced antioxidant activity and its DPPH scavenging rate reached about 86.05%.