Relevant bibliographies by topics / PAMP

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'PAMP'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 April 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'PAMP.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "PAMP"

1

Faye, Therese, Dag Anders Brede, Thor Langsrud, Ingolf F. Nes, and Helge Holo. "An Antimicrobial Peptide Is Produced by Extracellular Processing of a Protein from Propionibacterium jensenii." Journal of Bacteriology 184, no. 13 (July 1, 2002): 3649–56. http://dx.doi.org/10.1128/jb.184.13.3649-3656.2002.

Full text
Abstract:
ABSTRACT A protease-activated antimicrobial peptide (PAMP) and its inactive precursor were purified from the culture supernatant of Propionibacterium jensenii LMG 3032 and characterized at the molecular level. PAMP is a 64-amino-acid cationic peptide of 6,383 Da with physicochemical features similar to those of bacteriocins from gram-positive bacteria. This peptide displayed bactericidal activity against several propionibacteria and lactobacilli. DNA sequencing indicated that the PAMP-encoding gene (pamA) is translated as a proprotein of 198 amino acids with an N-terminal signal peptide of 27 amino acids and that PAMP constitutes the C-terminal part of this precursor. The amino acid sequence of pro-PAMP showed no similarity to those of other known proteins. By using activity tests and mass spectrometry, we showed that PAMP was formed upon protease treatment of the precursor protein. The propionibacteria produced the PAMP precursor constitutively during growth up to a level of ∼4 mg/liter, but the producing bacteria were unable to activate the precursor. The requirement for an external protease represents a novel strategy for generating antimicrobial peptides.
APA, Harvard, Vancouver, ISO, and other styles
2

Brede, Dag Anders, Therese Faye, Melanie Patricia Stierli, Gottfried Dasen, Anita Theiler, Ingolf F. Nes, Leo Meile, and Helge Holo. "Heterologous Production of Antimicrobial Peptides in Propionibacterium freudenreichii." Applied and Environmental Microbiology 71, no. 12 (December 2005): 8077–84. http://dx.doi.org/10.1128/aem.71.12.8077-8084.2005.

Full text
Abstract:
ABSTRACT Heterologous bacteriocin production in Propionibacterium freudenreichii is described. We developed an efficient system for DNA shuttling between Escherichia coli and P. freudenreichii using vector pAMT1. It is based on the P. freudenreichii rolling-circle replicating plasmid pLME108 and carries the cml(A)/cmx(A) chloramphenicol resistance marker. Introduction of the propionicin T1 structural gene (pctA) into pAMT1 under the control of the constitutive promoter (P4) yielded bacteriocin in amounts equal to those of the wild-type producer Propionibacterium thoenii 419. The P. freudenreichii clone showed propionicin T1 activity in coculture, killing 90% of sensitive bacteria within 48 h. The pamA gene from P. thoenii 419 encoding the protease-activated antimicrobial peptide (PAMP) was cloned and expressed in P. freudenreichii, resulting in secretion of the pro-PAMP protein. Like in the wild type, PAMP activation was dependent on externally added protease. Secretion of the antimicrobial peptide was obtained from a clone in which the pamA signal peptide and PAMP were fused in frame. The promoter region of pamA was identified by fusion of putative promoter fragments to the coding sequence of the pctA gene. The P4 and Ppamp promoters directed constitutive gene expression, and activity of both promoters was enhanced by elements upstream of the promoter core region.
APA, Harvard, Vancouver, ISO, and other styles
3

Veetil, Aneesh T., Junyi Zou, Katharine W. Henderson, Maulik S. Jani, Shabana M. Shaik, Sangram S. Sisodia, Melina E. Hale, and Yamuna Krishnan. "DNA-based fluorescent probes of NOS2 activity in live brains." Proceedings of the National Academy of Sciences 117, no. 26 (June 17, 2020): 14694–702. http://dx.doi.org/10.1073/pnas.2003034117.

Full text
Abstract:
Innate immune cells destroy pathogens within a transient organelle called the phagosome. When pathogen-associated molecular patterns (PAMPs) displayed on the pathogen are recognized by Toll-like receptors (TLRs) on the host cell, it activates inducible nitric oxide synthase (NOS2) which instantly fills the phagosome with nitric oxide (NO) to clear the pathogen. Selected pathogens avoid activating NOS2 by concealing key PAMPs from their cognate TLRs. Thus, the ability to map NOS2 activity triggered by PAMPs can reveal critical mechanisms underlying pathogen susceptibility. Here, we describe DNA-based probes that ratiometrically report phagosomal and endosomal NO, and can be molecularly programmed to display precise stoichiometries of any desired PAMP. By mapping phagosomal NO produced in microglia of live zebrafish brains, we found that single-stranded RNA of bacterial origin acts as a PAMP and activates NOS2 by engaging TLR-7. This technology can be applied to study PAMP−TLR interactions in diverse organisms.
APA, Harvard, Vancouver, ISO, and other styles
4

Lloyd, Simon R., Henk-jan Schoonbeek, Martin Trick, Cyril Zipfel, and Christopher J. Ridout. "Methods to Study PAMP-Triggered Immunity in Brassica Species." Molecular Plant-Microbe Interactions® 27, no. 3 (March 2014): 286–95. http://dx.doi.org/10.1094/mpmi-05-13-0154-fi.

Full text
Abstract:
The first layer of active defense in plants is based on the perception of pathogen-associated molecular patterns (PAMPs) leading to PAMP-triggered immunity (PTI). PTI is increasingly being investigated in crop plants, where it may have potential to provide durable disease resistance in the field. Limiting this work, however, is an absence of reliable bioassays to investigate PAMP responses in some species. Here, we present a series of methods to investigate PTI in Brassica napus. The assays allow measuring early responses such as the oxidative burst, mitogen-activated protein kinase phosphorylation, and PAMP-induced marker gene expression. Illumina-based RNA sequencing analysis produced a genome-wide survey of transcriptional changes upon PAMP treatment seen in both the A and C genomes of the allotetraploid B. napus. Later responses characterized include callose deposition and lignification at the cell wall, seedling growth inhibition, and PAMP-induced resistance to Pseudomonas syringae and Botrytis cinerea. Furthermore, using these assays, we demonstrated substantial variation in PAMP responses within a collection of diverse B. napus cultivars. The assays reported here could have widespread application in B. napus breeding and mapping programs to improve selection for broad-spectrum disease resistance.
APA, Harvard, Vancouver, ISO, and other styles
5

Yan, Yu, Dan Yao, and Xiaoyu Li. "Immunological Mechanism and Clinical Application of PAMP Adjuvants." Recent Patents on Anti-Cancer Drug Discovery 16, no. 1 (May 25, 2021): 30–43. http://dx.doi.org/10.2174/1574892816666210201114712.

Full text
Abstract:
Background: The host innate immune system can recognize Pathogen-Associated Molecular Patterns (PAMPs) through Pattern Recognition Receptors (PRRs), thereby initiating innate immune responses and subsequent adaptive immune responses. PAMPs can be developed as a vaccine adjuvant for modulating and optimizing antigen-specific immune responses, especially in combating viral infections and tumor therapy. Although several PAMP adjuvants have been successfully developed they are still lacking in general, and many of them are in the preclinical exploration stage. Objective: This review summarizes the research progress and development direction of PAMP adjuvants, focusing on their immune mechanisms and clinical applications. Methods: PubMed, Scopus, and Google Scholar were screened for this information. We highlight the immune mechanisms and clinical applications of PAMP adjuvants. Results: Because of the differences in receptor positions, specific immune cells targets, and signaling pathways, the detailed molecular mechanism and pharmacokinetic properties of one agonist cannot be fully generalized to another agonist, and each PAMP should be studied separately. In addition, combination therapy and effective integration of different adjuvants can increase the additional efficacy of innate and adaptive immune responses. Conclusion: The mechanisms by which PAMPs exert adjuvant functions are diverse. With continuous discovery in the future, constant adjustments should be made to build new understandings. At present, the goal of therapeutic vaccination is to induce T cells that can specifically recognize and eliminate tumor cells and establish long-term immune memory. Following immune checkpoint modulation therapy, cancer treatment vaccines may be an option worthy of clinical testing.
APA, Harvard, Vancouver, ISO, and other styles
6

Yan, Qing, Conner J. Rogan, and Jeffrey C. Anderson. "Development of a Pseudomonas syringae–Arabidopsis Suspension Cell Infection System for Investigating Host Metabolite-Dependent Regulation of Type III Secretion and Pattern-Triggered Immunity." Molecular Plant-Microbe Interactions® 32, no. 5 (May 2019): 527–39. http://dx.doi.org/10.1094/mpmi-10-18-0295-fi.

Full text
Abstract:
The importance of pattern-triggered immunity (PTI) in plant defense has been clearly established through genetic studies of mutants lacking functional pattern recognition receptors (PRRs) and signaling components downstream of PRR activation. Despite extensive knowledge of PRR-mediated signaling responses to pathogen-associated molecular patterns (PAMPs), little is known about which of these responses, if any, are directly responsible for limiting bacterial growth. In this work, we established a protocol for coculturing the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and Arabidopsis suspension cells. The system closely mirrors infection processes that occur in leaves, with bacteria relying on the type III secretion system (T3SS) for maximal growth and PAMP-induced plant defenses effectively limiting bacterial growth. To demonstrate the utility of this system, we investigated the molecular basis of PAMP-induced growth inhibition and discovered that T3SS-associated genes are inhibited when DC3000 is cocultured with PAMP-treated plant suspension cells. To determine the underlying mechanism of decreased T3SS gene expression, we performed metabolomics and biochemical analyses of suspension cell exudates and identified 14 metabolites that significantly increased or decreased following PAMP treatment. Citric acid, a known inducer of T3SS gene expression in DC3000, was among several organic acids decreased in exudates from PAMP-treated plant cells. Exogenous addition of citric acid increased T3SS gene expression and partially recovered growth of DC3000 in the presence of PAMP-treated cells, indicating that a portion of PAMP-induced defense in this system is decreased extracellular release of this metabolite. We envision that the well-defined infection conditions of this coculture system will be valuable for quantitative studies of type III effector delivery by P. syringae. Furthermore, this system provides a unique ‘top-down’ approach to unravel the molecular basis of PTI against P. syringae.
APA, Harvard, Vancouver, ISO, and other styles
7

Segers, Patrick, H. Alex Leather, Pascal Verdonck, Yuan-Yuan Sun, and Patrick F. Wouters. "Preload-adjusted maximal power of right ventricle: contribution of end-systolic P-V relation intercept." American Journal of Physiology-Heart and Circulatory Physiology 283, no. 4 (October 1, 2002): H1681—H1687. http://dx.doi.org/10.1152/ajpheart.00340.2002.

Full text
Abstract:
To assess whether preload-adjusted maximal power (PAMP), which is calculated asW˙max/V[Formula: see text] (whereW˙max is maximal power and Ved is end-diastolic volume with β = 2) is an index of right ventricular (RV) contractility, we measured RV pressure (P) and volume (V) and pulmonary artery pressure and flow in 10 dogs at baseline and after inotropic stimulation. PAMP was derived from steady-state data, whereas the slope ( E es) and intercept (Vd) of the end-systolic P-V relationship were derived from data obtained during vena caval occlusion. Inotropic stimulation increased E es (from 0.96 ± 0.25 to 1.62 ± 0.28 mmHg/ml; P < 0.001) and Vd (from −3.0 ± 17.2 to 12.4 ± 10.8 ml; P < 0.05) but not PAMP (from 0.24 ± 0.10 to 0.36 ± 0.22 mW/ml2; P = 0.09). We found a strong relationship between the optimal β-factor for preload adjustment and Vd. A corrected PAMP, PAMPc= W˙max/(Ved − Vd)2, which incorporated the Vddependency, was sensitive to the inotropic changes (from 0.23 ± 0.12 to 0.54 ± 0.17 mW/ml2; P < 0.001) with a good correlation with E es( r = 0.88; P < 0.001).
APA, Harvard, Vancouver, ISO, and other styles
8

Furukawa, Takehito, Hiroaki Inagaki, Ryota Takai, Hiroyuki Hirai, and Fang-Sik Che. "Two Distinct EF-Tu Epitopes Induce Immune Responses in Rice and Arabidopsis." Molecular Plant-Microbe Interactions® 27, no. 2 (February 2014): 113–24. http://dx.doi.org/10.1094/mpmi-10-13-0304-r.

Full text
Abstract:
Plants sense potential pathogens by recognizing conserved pathogen-associated molecular patterns (PAMPs) that cause PAMP-triggered immunity (PTI). We previously reported that rice recognizes flagellin from the rice-incompatible N1141 strain of Acidovorax avenae and subsequently induces immune responses. Cell extracts isolated from flagellin-deficient N1141 (Δfla1141) still induced PTI responses, suggesting that Δfla1141 possesses an additional PAMP distinct from flagellin. Here, we show that elongation factor Tu (EF-Tu), one of the most abundant bacterial proteins, acts as a PAMP in rice and causes several PTI responses. In Brassicaceae species, EF-Tu and an N-acetylated peptide comprising the first 18 amino acids of the N-terminus, termed elf18, are fully active as inducers of PTI responses. By contrast, elf18 did not cause any immune responses in rice, whereas an EF-Tu middle region comprising Lys176 to Gly225, termed EFa50, is fully active as a PAMP in rice. In the leaves of rice plants, EF-Tu induced H2O2 generation and callose deposition, and also triggered resistance to coinfection with pathogenic bacteria. Taken together, these data demonstrate that rice recognizes EFa50, which is distinct from elf18, and that this epitope induces PTI responses.
APA, Harvard, Vancouver, ISO, and other styles
9

Kanazawa, H., H. Kamoi, T. Kawaguchi, S. Shoji, T. Fujii, S. Kudoh, K. Hirata, and J. Yoshikawa. "PAMP is a novel inhibitor of the tachykinin release in the airway of guinea pigs." American Journal of Physiology-Lung Cellular and Molecular Physiology 272, no. 6 (June 1, 1997): L1066—L1069. http://dx.doi.org/10.1152/ajplung.1997.272.6.l1066.

Full text
Abstract:
Proadrenomedullin NH2-terminal 20 peptide (PAMP), a newly identified hypotensive peptide, may play physiological roles in airway and cardiovascular controls. This study was designed to determine the mechanism responsible for the bronchoprotective effects of PAMP on capsaicin-induced bron-choconstriction in anesthetized guinea pigs. PAMP (10(-8)-10(-6) M) significantly inhibited capsaicin-induced bronchoconstriction in a dose-dependent manner. The bronchoprotective effect of PAMP (10(-6) M) was as large as that of isoproterenol (10(-7) M) and lasted > 10 min. The concentration of immunoreactive substance P (SP) in bronchoalveolar lavage fluid after administration of capsaicin (4 x 10(-6) M) was 120 +/- 10 fmol/ml. PAMP significantly inhibited the release of immunoreactive SP in a dose-dependent manner (60 +/- 6 fmol/ml for (10(-6) M PAMP, P < 0.01; 84 +/- 6 fmol/ml for 10(-7) M PAMP, P < 0.01; and 95 +/- 6 fmol/ml for 10(-8) M PAMP, P < 0.05). PAMP (10(-6) M) did not significantly affect exogenous neurokinin A (NKA) or NKA + SP-induced bronchoconstriction, whereas isoproterenol (10(-7) M) significantly inhibited exogenous tachykinin-induced bronchoconstriction. These findings suggest that the bronchoprotective effects of PAMP are mainly due to inhibition of the release of tachykinins at airway C-fiber endings.
APA, Harvard, Vancouver, ISO, and other styles
10

Champion, H. C., W. A. Murphy, D. H. Coy, and P. J. Kadowitz. "Proadrenomedullin NH2-terminal 20 peptide has direct vasodilator activity in the cat." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 272, no. 4 (April 1, 1997): R1047—R1054. http://dx.doi.org/10.1152/ajpregu.1997.272.4.r1047.

Full text
Abstract:
The mechanism by which proadrenomedullin NH2-terminal 20 peptide (PAMP) decreases vascular resistance was investigated in the hindlimb vascular bed in the cat. Injections of PAMP, a shortened form of the peptide PAMP-(12-20), and adrenomedullin (ADM) into the hindlimb perfusion circuit elicit dose-related decreases in perfusion pressure. The order of potency was ADM > PAMP > PAMP-(12-20), and the calcitonin gene-related peptide (CGRP) receptor antagonist CGRP-(8-37) had no effect on vasodilator responses to PAMP or ADM. Vasodilator responses to PAMP were increased in duration by the adenosine 3',5'-cyclic monophosphate (cAMP) phosphodiesterase inhibitor Rolipram, whereas inhibitors of nitric oxide synthase and guanosine 3',5'-cyclic monophosphate phosphodiesterase had no effect. Vasodilator responses to PAMP were not altered by treatment with alpha-receptor or adrenergic nerve terminal blocking agents and were similar in innervated and denervated hindlimb preparations. Responses to PAMP were similar when vasoconstrictor tone was increased by stimulation of the sympathetic nerves or infusion of phenylephrine and were not altered by the passage of time. These data suggest that PAMP dilates the hindlimb vascular bed by a direct cAMP-dependent mechanism and that inhibition of norepinephrine release plays little if any role in mediating responses to the peptide in the regional vascular bed of the cat.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "PAMP"

1

Thomson, Laura Margaret. "Adrenomedullin, PAMP and adrenocortical function." Thesis, Queen Mary, University of London, 2001. http://qmro.qmul.ac.uk/xmlui/handle/123456789/25124.

Full text
Abstract:
Adrenomedullin (AM) and pro-adrenomedullin N-terminal 20-peptide (PAMP) are peptides recently identified from a rat pheochromocytoma. Both of these peptides are cleavage products of pre-pro-AM. Specific receptors for AM have been characterised in several species, including rat and human. The aim of this study was to investigate the role of PAMP and AM in the adrenal cortex. Using an intact rat capsule preparation PAMP was shown to cause a dosedependent increase in aldosterone secretion, which was accompanied by a dosedependent increase in cAMP release. The effects of PAMP were inhibited by HA1004, an inhibitor of protein kinase A. These results suggest that PAMP stimulates aldosterone secretion from the zona glomerulosa via cAMP. Ligandbinding studies were then used to demonstrate the presence of specific PAMP receptors. Two classes of receptor were shown in the rat zona glomerulosa (Kdi 1.9 nmol/l, Bmai, 53 fmol/mg protein; Kd2 10 nmol/l, Bmac,2 225 fmol/mg protein). At the latter receptor PAMP was displaced by AM. None of the other competitors tested displaced PAMP. Using the H295R cell line, both PAMP and AM were shown to increase aldosterones ecretion in a dose-dependenmt anner. In both casesa corresponding dose-dependent increase in cAMP was observed. Both PAMP and AM also effected a dose dependent increase in cortisol secretion. mRNA analysis showed that the gene encoding pre-pro-AM was expressed in these cells. Immunocytochemistry confirmed that these cells were producing both PAMP and AM. Immunocytochemistry and mRNA analysis also revealed that both of the candidate receptors for AM, L1 and CRLR, are expressed in this cell line. Taken together these findings demonstrate that both AM and PAMP are produced by adrenocortical cells and likely to have a role in regulating adrenal steroidogenesis. Furthermore, these studies suggest the presence of a specific PAMP receptor in the rat adrenal gland.
APA, Harvard, Vancouver, ISO, and other styles
2

Lloyd, Simon. "Mapping PAMP responses and disease resistance in brassicas." Thesis, University of East Anglia, 2014. https://ueaeprints.uea.ac.uk/49479/.

Full text
Abstract:
The first layer of active defence in plants is based on the perception of pathogen-associated molecular patterns (PAMPs) leading to PAMP-triggered immunity (PTI). PTI is increasingly being investigated in crop plants, where it may have potential to provide durable disease resistance in the field. Limiting this work, however, is an absence of reliable bioassays to investigate PAMP responses in some species. Presented here is a series of methods to investigate PTI in Brassica napus. The assays allow measuring early cell signalling responses, gene expression changes, cell wall reinforcement, metabolome changes and scoring PAMP-Induced resistance. Illumina-based RNA sequencing analysis produced a genome-wide survey of transcriptional changes upon PAMP treatment seen in both the A and C genomes of the allotetraploid B. napus. Using these assays substantial variation in PAMP-responsiveness was observed amongst elite varieties of B. napus. Taking this further, a genome wide association study (GWAS) of the flg22 and elf18 triggered oxidative bursts, resistance to Pseudomonas syringae and Botrytis cinerea was carried out in a population of B. napus. A substantial number of molecular markers, covering both sequence and expression variation, have been identified as having significant association with these four traits. QTL mapping of the flg22 triggered oxidative burst in the ADxGD double haploid cross identified a major quantitative trait loci (QTL) on C9 of B. oleracea. mRNA-seq of the parents led to a non-synonymous single nucleotide polymorphism (SNP) list and enabled fine mapping through the addition of KASPar markers to the original map. This produced a relatively small list of candidate genes including CYLIC NUCLEOTIDE GATED ION CHANNEL 4 (CNGC4) also known as DEFENCE NO DEATH 2 (DND2). An insertion in Arabidopsis thaliana DND2 showed phenotypic difference in the oxidative burst between the insertion line and Col-0, potentially indicating a role for the gene in regulating early PTI.
APA, Harvard, Vancouver, ISO, and other styles
3

Niebergall, Roda. "Characterisation of potential regulators of PAMP-triggered immunity." Thesis, University of East Anglia, 2012. https://ueaeprints.uea.ac.uk/42367/.

Full text
Abstract:
An essential part of plant innate immunity is the perception of pathogen-associated molecular patterns (PAMPs) through surface-localised pattern-recognition receptors (PRRs). EFR and FLS2 are well characterised PRRs, which recognise the bacterial elongation factor Tu and flagellin, respectively. A variety of downstream responses upon PAMP perception have been described. However, it is still poorly understood how EFR- and FLS2-dependent signalling is mediated. Here, I used two different approaches in order to identify novel components of both signalling pathways. First, I characterised seven candidate genes of a predicted flagellin-dependent gene expression network, which are predicted to regulate each other’s expression in a flagellin-dependent manner, and therefore, are potentially involved in the FLS2 signalling pathway. Further characterisation revealed that mutation of at least three of the seven candidate genes was affecting flg22-mediated signalling. In addition, I characterised a predicted protein phosphatase 2C (PP2C), which had been identified in a yeast two-hybrid screen with the EFR cytoplasmic domain and was therefore referred to as PIE (PP2C-interacting with EFR). Using Co-IP experiments, I confirmed that PIE associates with EFR in planta. Furthermore, PIE also associates with FLS2 and BIK1, a co-regulator of both PRRs, in planta. Interestingly, PIE dissociates from both the EFR and FLS2 complexes upon PAMP perception. PIE expressed in planta and in E. coli exhibits protein phosphatase activity and we showed that PIE dephosphorylates EFR, FLS2 and BIK1 in vitro. As expected from bio-informatic predictions, I confirmed that PIE is phosphorylated upon PAMP perception. Furthermore, I demonstrated that EFR and FLS2 kinase activities are partially required for PIE phosphorylation. Both PIE overexpression and pie knock-down lines display reduced PAMP-triggered responses, indicating that an optimal PIE expression level is required for proper induction of signalling. All together, these results imply that PIE is a novel regulator of the EFR and FLS2 signalling pathway.
APA, Harvard, Vancouver, ISO, and other styles
4

Masini, Laura. "Investigation of the molecular basis of PAMP-induced resistance." Thesis, University of East Anglia, 2014. https://ueaeprints.uea.ac.uk/58753/.

Full text
Abstract:
The recognition of conserved microbial features termed pathogen-associated molecular patterns (PAMPs) by surface-localized pattern-recognition receptors (PRRs) constitutes the first layer of plant innate immunity. Although details of early immune signaling events are starting to be unveiled, the molecular mechanisms leading to restriction of pathogen growth are still poorly understood. To gain more insight into this process, two different approaches were employed. I used reverse genetics to study the involvement of three different secondary metabolites, namely camalexin, glucosinolates and callose, in PAMP-triggered immunity (PTI) against the phytopathogenic bacterium Pseudomonas syringae pv. tomato (Pto) DC3000. These are well known active defences Arabidopsis employs to restrict fungi and oomycetes invasion. Results showed that these compounds are dispensable for antibacterial resistance triggered by the bacterial PAMP flagellin (flg22). In addition, as an unbiased approach, I performed a novel genetic screen aimed at identifying molecular components required for induced resistance to Pto DC3000. For this, I developed a high-throughput assay for bacterial infection in Arabidopsis seedlings that enabled to select mutants impaired in flg22-induced resistance to Pto DC3000. The pir (PAMP-induced resistance) screen identified four loci whose mutation leads to a reproducible reduction of flg22-induced resistance. These genes have not been previously characterized for their role in immunity, and therefore can be considered as novel components of PTI. By employing a combination of reverse genetics, metabolomics and chemistry approaches, I obtained preliminary data suggesting that flavonoids act as cellular buffers and/or are employed as active defenses against bacteria. In addition, interference with the mevalonic acid biosynthetic pathway impairs antibacterial defenses, suggesting a role in immunity. Additional tests are underway to better assess the contribution of these PIR genes to PTI. Therefore, through the pir screen, I have identified several novel loci required for plant immunity that will increase our knowledge of the plant immune system.
APA, Harvard, Vancouver, ISO, and other styles
5

Nitta, Yukino. "Regulation of plant defense responses downstream of PAMP receptors." Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/51596.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Nativel, Brice. "Pathologie inflammatoire : étude de la contribution des PAMP et DAMP." Thesis, La Réunion, 2017. http://www.theses.fr/2017LARE0065.

Full text
Abstract:
L’inflammation est le mécanisme de base du système immunitaire. Dans le cas de pathologie inflammatoire cette inflammation persiste et devient délétère pour l’organisme. Les causes de cette persistance peuvent être variées. L’une de ces causes est la présence de molécules induisant l’inflammation. Elles peuvent être d’origine exogène comme les PAMP (Pathogen-Associated Molecular Pattern). Ce sont des molécules issues des pathogènes (LPS, peptidoglycanes, ADN CpG) capables d’activer le système immunitaire. Ces molécules peuvent également être d’origine endogène comme les DAMP (Damage Associated Molecular Pattern). Ce sont des molécules libérées par les cellules en état de danger (HMGB1, HSP60) pour prévenir et activer le système immunitaire. La présence de récepteurs (TLR2, TLR4, RAGE) capable de reconnaitre ces PAMP et DAMP est également nécessaire pour pouvoir induire une inflammation. Mes travaux explorent les mécanismes moléculaires et cellulaires des PAMP et des DAMP, dans l’installation et le maintien de l’inflammation dans le cadre des maladies inflammatoires. Pour cela mon étude se focalise sur les mécanismes de reconnaissance et d’induction de l’inflammation par les PAMP et DAMP. Nous avons ainsi mis en évidence certains mécanismes cellulaires et moléculaires dans la réponse inflammatoire liés aux DAMP et PAMP. Nous nous sommes également intéressé aux récepteurs impliqués dans ces différents mécanismes et avons même mis en évidence un potentiel nouveau récepteur CD93. Nous émettons l’hypothèse que CD93 pourrait avoir un rôle dans les pathologies inflammatoires par cette capacité à lier les DAMP et PAMP
Inflammation is the basic mechanism of the immune system. In the case of inflammatory pathologies this inflammation persists and becomes deleterious to the organism. Many reasons can explain this persistance. One of these causes is the presence of inflammatory-inducing molecules. They may have exogenous origin such as PAMP (Pathogen Associated Molecular Pattern). They are derived from pathogens (LPS, peptidoglycans, CpG DNA ...), and are able to activate the immune system. These molecules can also have endogenous origin such as the DAMP (Damage Associated Molecular Pattern). They are released by stress cells (HMGB1, HSP60, S100 ...) to prevent and activate the immune system. The presence of receptors (TLR2, TLR4, RAGE ...) capable of recognizing these PAMPs and DAMPs is also necessary in order to elicit inflammation.My work explores the contribution of PAMPs and DAMPs to inflammatory diseases at molecular and cellular levels. To this end, my study focuses on recognition and induction of inflammation by PAMPs and DAMPs.We have thus demonstrated cellular and molecular mechanisms in the inflammatory response related to DAMP and PAMP. We were also interested in the receptors involved in these mechanisms and even showed a new potential receptor. We hypothesize that CD93 may have a role in inflammatory pathologies by his ability to bind DAMPs and PAMPs. Thus CD93, HMGB1, HSP60 and LPS could be potential therapeutic targets concerning inflammatory diseases
APA, Harvard, Vancouver, ISO, and other styles
7

Schwessinger, Benjamin. "Genetic analysis of signalling components of PAMP-triggered immunity (PTI) in plants." Thesis, University of East Anglia, 2010. https://ueaeprints.uea.ac.uk/25632/.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Rallapalli, Ghanasyam. "Sequencing based expression profiling to dissect transcriptional regulation during PAMP-triggered immunity." Thesis, University of East Anglia, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.539357.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Gäbler, Yvonne. "Identifizierung und Charakterisierung von PAMP-induzierbaren Genen in Petroselinum crispum und Arabidopsis thaliana." [S.l. : s.n.], 2007.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Doñate, Jimeno Carmen. "A transcriptomic approach toward understanding PAMP-driven macrophage activation and dietary immunostimulation in fish." Doctoral thesis, Universitat Autònoma de Barcelona, 2008. http://hdl.handle.net/10803/3816.

Full text
Abstract:
Los peces son claramente el grupo más exitoso y diverso de vertebrados, representando el 40% de todas las especies de vertebrados y mostrando un impresionante nivel de diversidad en distintos aspectos biológicos. Exhiben un gran número de particularidades genómicas únicas entre los vertebrados, que presentan a los peces como un modelo muy interesante para diversas disciplinas, en particular aquellas relacionas con la evolución. Por estas razones, algunas especies de peces han tenido un papel muy importante en los últimos años en el incremento del conocimiento de la especialización del genoma en vertebrados. Por otra parte, tienen una importancia vital como comida, siendo la acuicultura un sector productor alimentario esencial en todo el mundo. El objetivo del presente trabajo ha sido caracterizar ciertos aspectos moleculares y funcionales del sistema inmune de dos especies distantes en la evolución, Sparus aurata (dorada) y Oncorhynchus mykiss (trucha arco iris), con especial énfasis en sus respuestas transcriptómicas a diferentes estímulos relativos a patógenos. Con esta finalidad, análisis in vivo e in vitro fueron combinados para evaluar mecanismos inmunitarios globales de estos teleósteos.
Los Macrófagos representan un grupo importante de células que poseen un papel principal en la iniciación y coordinación de la respuesta inmune. Se desarrolló y caracterizó un cultivo primario de macrófagos diferenciados in vitro de dorada, investigando aspectos como morfología, capacidad fagocítica y respuesta a lipopolisacárido (LPS) de este tipo celular. En paralelo, CD83, una proteína de membrana utilizada como marcador estándar de células dendríticas en mamíferos fue clonada y analizada usando Q-PCR (PCR a tiempo real, cuantitativa). A continuación, los macrófagos diferenciados de dorada fueron comparados con los de trucha, evaluando sus diferencias en la activación de vías antivirales bajo la inducción de LPS y las implicaciones de la presencia de contaminantes en preparaciones comerciales de LPS. La expresión de varios genes antivirales fue cuantificada mediante Q-PCR. Para analizar más profundamente las respuestas inmunes de macrófagos, su regulación transcriptómica en respuesta a LPS bacteriano y Poly (I:C) viral fue estudiada utilizando una plataforma de microarray de cDNA enriquecida en genes con funciones inmunes, resultados que fueron posteriormente validados con Q-PCR, junto con el análisis mediante western blot de la liberación de la citoquina inflamatoria Factor de Necrosis Tumoral α (TNFα). Finalmente, la regulación de cortisol y la respuesta trancriptómica de los teleósteos a una modulación inmune fue evaluada a través de la administración de dietas inmunoestimulantes, que son comúnmente utilizadas en acuicultura. A través de diversos análisis, utilizando la plataforma de microarray, hibridaciones in situ y cuantificación de los niveles de cortisol en plasma por R.I.A., estudiamos respuestas específicas en tejidos (riñón anterior, bazo, intestino y branquias) en truchas alimentadas durante cuatro semanas con una dieta inmunoestimulante, en condicones basales y siguiendo una inducción con LPS. Los resultados obtenidos han sido presentados y discutidos en este trabajo.
Fish are by far the most successful and diverse group of vertebrates, representing 40% of all vertebrate species and displaying an amazing level of diversity in several biological aspects. They exhibit a number of genomic particularities unique among vertebrates that present fish as a very interesting model to gain an insight into a wide variety of disciplines, in particular those related to evolution. Therefore some fish species have played important roles in the latest years to increase the knowledge of vertebrate genome speciation. On the other hand, they are of tremendous importance as food for people, becoming the aquaculture industry an essential food-producing sector all around the world. The goal of the present study has been to characterize several molecular and functional aspects of the immune system of two evolutionary distant fish species, Sparus aurata (gilthead sea bream) and Oncorhynchus mykiss (rainbow trout), with specific emphasis on their transcriptomic responses to different pathogen-related challenges. To that end, in vivo and in vitro analyses were combined to evaluate global immune mechanisms of these teleosts.
The macrophage cell lineage represents an important group of cells which play a central role in the initiation and coordination of the immune response. A primary culture of in vitro differentiated macrophages of gilthead sea bream was developed and characterized; therefore aspects as morphology, phagocytic capacity and response to lipopolysaccharides (LPS) of these cells were investigated. In parallel, CD83, a cell surface membrane used as standard surface marker for dendritic cells in mammals, was cloned and then analyzed from the gilthead sea bream macrophages using Q-PCR (real-time quantitative-PCR). Once this in vitro model was characterized and validated, differentiated macrophages of gilthead sea bream were compared with those of rainbow trout to evaluate their differences in the activation of antiviral-related pathways upon LPS induction and the implications of the presence of contaminants in commercial LPS preparations when analyzing regulation of gene expression. Expression of antiviral genes in macrophages stimulated with different LPS preparations were quantified with Q-PCR. To further address rainbow trout macrophages immune responses, their transcriptomic regulation in response to bacterial LPS and viral Poly I:C was studied using a salmonid-specific cDNA microarray platform enriched in immune-related genes and validated with Q-PCR, together with the analysis of the release of the pro-inflammatory cytokine tumor necrosis factor α (TNFα) by western blot. Finally, the cortisol regulation and transcriptomic response of teleost fish to immuno-modulation were investigated via the administration of immunostimulant diets, which are commonly utilized in aquaculture. Using the salmonid-specific microarray platform, in situ hybridizations and quantification of plasma cortisol levels by radioimmunoassay, we studied tissue specific (head kidney, spleen, intestine and gills) responses in rainbow trout fed for four weeks with a commercial immunostimulant diet, in a basal situation and following a challenge with LPS. The results obtained are presented and discussed in this report.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "PAMP"

1

Vidhyasekaran, P. PAMP Signals in Plant Innate Immunity. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-7426-1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Lif, Anders. Direktörernas direktör: Sigfrid Edström - ASEA-chef, SAF-bas och OS-pamp. Stockholm: Atlantis, 2015.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Sastry, T. V. Venkatachala. Pampa. Navadehali: Sāhitya Akādemi, 1987.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

Endrigo, Edson. Aves do pampa =: Birds : pampa. [São Paulo, Brazil]: Aves & Fotos Editora, 2010.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Ibang chidae ŭi pam, pam, pam? Sŏul-si: Samwŏn Chʻulpʻansa, 1989.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Suvīr, Lī. Qariyadham̊ Khmaer: Dâkdaṅ niṅ qaksārsilpaḥ -paep Buddhniyam-paep Braṃniyam-paep Khmaerniyam. 2nd ed. [Phnom Penh: s.n.], 2007.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Schvartz, Claudia. Pampa argentino. Buenos Aires, R. Argentina: Ediciones Ultimo Reino, 1989.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Ayyappan, E. Māḷamillātta pāmp. Thiruvananthapuram: Focus Books, 1992.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Pampa: Roman. Paris: Seuil, 2007.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Theodorakis, M. Pou pame? Athens: Gnosis, 1989.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "PAMP"

1

Azimova, Shakhnoza S., and Anna I. Glushenkova. "Artemisia samoiedorum Pamp." In Lipids, Lipophilic Components and Essential Oils from Plant Sources, 90. London: Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_292.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Azimova, Shakhnoza S., and Anna I. Glushenkova. "Artemisia umbrosa (Bess.) Pamp." In Lipids, Lipophilic Components and Essential Oils from Plant Sources, 100. London: Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_323.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Vidhyasekaran, P. "PAMP Signaling in Plant Innate Immunity." In PAMP Signals in Plant Innate Immunity, 17–161. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-7426-1_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Azimova, Shakhnoza S., and Anna I. Glushenkova. "Artemisia santolinifolia (Pamp.) Turcz. ex Krasch." In Lipids, Lipophilic Components and Essential Oils from Plant Sources, 90. London: Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_293.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Vidhyasekaran, P. "Introduction." In PAMP Signals in Plant Innate Immunity, 1–16. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7426-1_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Vidhyasekaran, P. "Ubiquitin-Proteasome System-Mediated Protein Degradation in Defense Signaling." In PAMP Signals in Plant Innate Immunity, 409–30. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7426-1_10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Vidhyasekaran, P. "G-Proteins as Molecular Switches in Signal Transduction." In PAMP Signals in Plant Innate Immunity, 163–205. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7426-1_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Vidhyasekaran, P. "Calcium Ion Signaling System: Calcium Signatures and Sensors." In PAMP Signals in Plant Innate Immunity, 207–82. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7426-1_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Vidhyasekaran, P. "Reactive Oxygen Species and Cognate Redox Signaling System in Plant Innate Immunity." In PAMP Signals in Plant Innate Immunity, 283–306. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7426-1_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Vidhyasekaran, P. "Nitric Oxide Signaling System in Plant Innate Immunity." In PAMP Signals in Plant Innate Immunity, 307–29. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7426-1_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "PAMP"

1

Chih-Yung Chang, Kuei-Ping Shih, Hsu-Jui Chang, Shih-Chieh Lee, and Gwo-Jong Yu. "PAMP: a power-aware multicast protocol for Bluetooth radio systems." In 2004 International Conference on Communications, Circuits and Systems. IEEE, 2004. http://dx.doi.org/10.1109/icccas.2004.1346102.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

DASGUPTA, BHASKAR, JIN JUN, and ION I. MĂNDOIU. "PRIMER SELECTION METHODS FOR DETECTION OF GENOMIC INVERSIONS AND DELETIONS VIA PAMP." In The 6th Asia-Pacific Bioinformatics Conference. PUBLISHED BY IMPERIAL COLLEGE PRESS AND DISTRIBUTED BY WORLD SCIENTIFIC PUBLISHING CO., 2007. http://dx.doi.org/10.1142/9781848161092_0036.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Yang, Jin Seok, Kyungran Kang, Young-Jong Cho, and Sung Yoon Chae. "PAMP: Power-Aware Multi-Path Routing Protocol for a Wireless Ad Hoc Network." In 2008 IEEE Wireless Communications and Networking Conference. IEEE, 2008. http://dx.doi.org/10.1109/wcnc.2008.397.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Zhou, Yang, Min-Jong Kang, Robert H. Silverman, Chun G. Lee, and Jack A. Elias. "Role Of Rnasel In Virus/Viral PAMP And Cigarette Smoke-Induced Inflammation And Remodeling." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a2305.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Gentry, Michael F., and Norman M. Wereley. "Effects of Braid Angle on Pneumatic Artificial Muscle Actuator Performance." In ASME 2008 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2008. http://dx.doi.org/10.1115/smasis2008-539.

Full text
Abstract:
Pneumatic artificial muscles (PAMs) provide numerous advantages for use as actuators in a wide variety of mechanical systems. Our study focused on determining the effects of braid angle on the performance of PAMs. This paper discusses how we constructed a set of PAMs with varying braid angle, predicted their performance using analytical models, gathered empirical data characterizing the PAMs, and compared the analytical predictions with the experimental results. We constructed six PAMs of different braid angles between 38° and 73°. To predict PAM performance, we used an analysis based on the force equilibrium equations for a pressurized actuator. We first quantified the performance limits of each actuator in a series of static characterization tests. Then we subjected each PAM to cyclical displacement testing. Finally, a series of cyclical tests were performed with a pre-strain applied to the PAMs, to better approximate their typical use. Our results showed variation of braid angle causes significant differences in performance among the six PAMs tested; PAMs with larger braid angle generated higher blocked force and exhibited greater contraction. The empirical data matched the model predictions based on our estimates for the braid angle of a given PAM.
APA, Harvard, Vancouver, ISO, and other styles
6

Krishnan, Aravind. "A vel Assay to Quantitatively Detect Bacterial Endotoxin by Harnessing PAMP-Triggered Immunity of FRK1-LUC Arabidopsis thaliana." In ASPB PLANT BIOLOGY 2020. USA: ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1376629.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Tun, Aung M., Stephen Hefeneider, Sharon McCoy, Erin Danielson, and Jeffrey A. Gold. "Synthetic Peptides Derived From Vaccinia Virus Inhibit Pathogen Associated Molecular Patterns (PAMP) Induced Nf-kB Activation In Human Macrophages." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a2501.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Pillsbury, Thomas E., Ryan M. Robinson, and Norman M. Wereley. "Miniaturized Pneumatic Artificial Muscles Actuating a Bio-Inspired Robot Hand." In ASME 2013 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/smasis2013-3262.

Full text
Abstract:
Pneumatic artificial muscles (PAMs) are used in robotics applications for their light-weight design and superior static performance. Additional PAM benefits are high specific work, high force density, simple design, and long fatigue life. Previous use of PAMs in robotics research has focused on using “large,” full-scale PAMs as actuators. Large PAMs work well for applications with large working volumes that require high force and torque outputs, such as robotic arms. However, in the case of a compact robotic hand, a large number of degrees of freedom are required. A human hand has 35 muscles, so for similar functionality, a robot hand needs a similar number of actuators that must fit in a small volume. Therefore, using full scale PAMs to actuate a robot hand requires a large volume which for robotics and prosthetics applications is not feasible, and smaller actuators, such as miniature PAMs, must be used. In order to develop a miniature PAM capable of producing the forces and contractions needed in a robotic hand, different braid and bladder material combinations were characterized to determine the load stroke profiles. Through this characterization, miniature PAMs were shown to have comparably high force density with the benefit of reduced actuator volume when compared to full scale PAMs. Testing also showed that braid-bladder interactions have an important effect at this scale, which cannot be modeled sufficiently using existing methods without resorting to a higher-order constitutive relationship. Due to the model inaccuracies and the limited selection of commercially available materials at this scale, custom molded bladders were created. PAMs created with these thin, soft bladders exhibited greatly improved performance.
APA, Harvard, Vancouver, ISO, and other styles
9

Vocke, Robert D., Curt S. Kothera, and Norman M. Wereley. "Mechanism and Bias Considerations for Design of a Bi-Directional Artificial Muscle Actuator." In ASME 2012 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/smasis2012-8114.

Full text
Abstract:
Pneumatic artificial muscles (PAMs), or McKibben actuators, have received considerable attention for robotic manipulators and in aerospace applications due to their similarity to natural muscles. Like natural muscles, PAMs are a purely contractile actuator, so that, in order to produce bidirectional or rotational motion, they must be arranged in an agonist/antagonist pair, which inherently limits the deflection of the system due to the high parasitic stiffness of the antagonistic PAM. This study presents two methods for increasing the performance of an antagonistic PAM system by decreasing the passive parasitic moment, rather than increasing the active moment. The first involves selection of the kinematic mechanism geometry, and the second involves the introduction of bias into the system, both in terms of PAM contraction, and passive (antagonistic) PAM pressure. It was found with the proper selection of design parameters, including mechanism geometry, PAM geometry, and bias conditions, that an ideal actuator configuration can be found that maximizes deflection for a given arbitrary loading. When comparing a baseline design to an improved design for a simplified case, a nearly 50% increase in maximum deflection was predicted simply by optimizing mechanism geometry and bias contraction.
APA, Harvard, Vancouver, ISO, and other styles
10

Agler, Anne H., Brent S. Pedersen, Laura A. Warg, David A. Schwartz, and Ivana V. Yang. "Whole Genome Association Mapping To Identify Novel Innate Immunity Genes Using Macrophage Cytokine Response To Pathogen-Associated Molecular Patterns (PAMP)." In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a1063.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "PAMP"

1

John l. Loth, Gary J. Morris, George M. Palmer, Richard Guiler, and Deepak Mehra. PORTABLE ACOUSTIC MONITORING PACKAGE (PAMP). Office of Scientific and Technical Information (OSTI), July 2003. http://dx.doi.org/10.2172/822766.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

John L. Loth, Gary J. Morris, George M. Palmer, Richard Guiler, and Patrick Browning. PORTABLE ACOUSTIC MONITORING PACKAGE (PAMP). Office of Scientific and Technical Information (OSTI), July 2004. http://dx.doi.org/10.2172/828277.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Sperry, Jason L., Barbara Early, and Meghan Buck. PUPTH Prehospital Air Medical Plasma (PAMP) Trial. Fort Belvoir, VA: Defense Technical Information Center, July 2014. http://dx.doi.org/10.21236/ada612334.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Sperry, Jason L., Barbara Early, and Meghan Buck. PUPTH Prehospital Air Medical Plasma (PAMP) Trial. Fort Belvoir, VA: Defense Technical Information Center, July 2013. http://dx.doi.org/10.21236/ada612541.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

John L. Loth, Gary J. Morris, George M. Palmer, Richard Guiler, and Patrick Browning. OPERATING PROCEDURE FOR THE PORTABLE ACOUSTIC MONITORING PACKAGE (PAMP). Office of Scientific and Technical Information (OSTI), August 2004. http://dx.doi.org/10.2172/834111.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Sessa, Guido, and Gregory B. Martin. molecular link from PAMP perception to a MAPK cascade associated with tomato disease resistance. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597918.bard.

Full text
Abstract:
The research problem: The detection of pathogen-associated molecular patterns (PAMPs) by plant pattern recognition receptors (PRRs) is a key mechanism by which plants activate an effective immune response against pathogen attack. MAPK cascades are important signaling components downstream of PRRs that transduce the PAMP signal to activate various defense responses. Preliminary experiments suggested that the receptor-like cytoplasmickinase (RLCK) Mai5 plays a positive role in pattern-triggered immunity (PTI) and interacts with the MAPKKK M3Kε. We thus hypothesized that Mai5, as other RLCKs, functions as a component PRR complexes and acts as a molecular link between PAMP perception and activation of MAPK cascades. Original goals: The central goal of this research was to investigate the molecular mechanisms by which Mai5 and M3Kε regulate plant immunity. Specific objectives were to: 1. Determine the spectrum of PAMPs whose perception is transmitted by M3Kε; 2. Identify plant proteins that act downstream of M3Kε to mediate PTI; 3. Investigate how and where Mai5 interacts with M3Kε in the plant cell; 4. Examine the mechanism by which Mai5 contributes to PTI. Changes in research directions: We did not find convincing evidence for the involvement of M3Kε in PTI signaling and substituted objectives 1 and 3 with research activities aimed at the analysis of transcriptomic profiles of tomato plants during the onset of plant immunity, isolation of the novel tomato PRR FLS3, and investigation of the involvement of the RLCKBSKs in PTI. Main achievements during this research program are in the following major areas: 1. Functional characterization of Mai5. The function of Mai5 in PTI signaling was demonstrated by testing the effect of silencing the Mai5 gene by virus-induced gene silencing (VIGS) experiments and in cell death assays. Domains of Mai5 that interact with MAPKKKs and subcellular localization of Mai5 were analyzed in detail. 2. Analysis of transcriptional profiles during the tomato immune responses to Pseudomonas syringae (Pombo et al., 2014). We identified tomato genes whose expression is induced specifically in PTI or in effector-triggered immunity (ETI). Thirty ETI-specific genes were examined by VIGS for their involvement in immunity and the MAPKKK EPK1, was found to be required for ETI. 3. Dissection of MAP kinase cascades downstream of M3Kε (Oh et al., 2013; Teper et al., 2015). We identified genes that encode positive (SGT and EDS1) and negative (WRKY1 and WRKY2) regulators of the ETI-associated cell death mediated by M3Kε. In addition, the MKK2 MAPKK, which acts downstream of M3Kε, was found to interact with the MPK3 MAPK and specific MPK3 amino acids involved interaction were identified and found to be required for induction of cell death. We also identified 5 type III effectors of the bacterial pathogen Xanthomonaseuvesicatoria that inhibited cell death induced by components of ETI-associated MAP kinase cascades. 4. Isolation of the tomato PRR FLS3 (Hind et al., submitted). FLS3, a novel PRR of the LRR-RLK family that specifically recognizes the flagellinepitope flgII-28 was isolated. FLS3 was shown to bind flgII-28, to require kinase activity for function, to act in concert with BAK1, and to enhance disease resistance to Pseudomonas syringae. 5. Functional analysis of RLCKs of the brassinosteroid signaling kinase (BSK) family.Arabidopsis and tomato BSKs were found to interact with PRRs. In addition, certain ArabidospsisBSK mutants were found to be impaired in PAMP-induced resistance to Pseudomonas syringae. Scientific and agricultural significance: Our research activities discovered and characterized new molecular components of signaling pathways mediating recognition of invading pathogens and activation of immune responses against them. Increased understanding of molecular mechanisms of immunity will allow them to be manipulated by both molecular breeding and genetic engineering to produce plants with enhanced natural defense against disease.
APA, Harvard, Vancouver, ISO, and other styles
7

Sessa, Guido, and Gregory Martin. role of FLS3 and BSK830 in pattern-triggered immunity in tomato. United States Department of Agriculture, January 2016. http://dx.doi.org/10.32747/2016.7604270.bard.

Full text
Abstract:
Pattern-recognition receptors (PRRs) located on the plant cell surface initiate immune responses by perceiving conserved pathogen molecules known as pathogen-associated molecular patterns (PAMPs). PRRs typically function in multiprotein complexes that include transmembrane and cytoplasmickinases and contribute to the initiation and signaling of pattern-triggered immunity (PTI). An important challenge is to identify molecular components of PRR complexes and downstream signaling pathways, and to understand the molecular mechanisms that mediate their function. In research activities supported by BARD-4931, we studied the role of the FLAGELLIN SENSING 3 (FLS3) PRR in the response of tomato leaves to flagellin-derivedPAMPs and PTI. In addition, we investigated molecular properties of the tomato brassinosteroid signaling kinase 830 (BSK830) that physically interacts with FLS3 and is a candidate for acting in the FLS3 signaling pathway. Our investigation refers to the proposal original objectives that were to: 1) Investigate the role of FLS3 and its interacting proteins in PTI; 2) Investigate the role of BSK830 in PTI; 3) Examine molecular and phosphorylation dynamics of the FLS3-BSK830 interaction; 4) Examine the possible interaction of FLS3 and BSK830 with Pstand Xcveffectors. We used CRISPR/Cas9 techniques to develop plants carrying single or combined mutations in the FLS3 gene and in the paralogsFLS2.1 and FLS2.2 genes, which encode the receptor FLAGELLIN SENSING2 (FLS2), and analyzed their function in PTI. Domain swapping analysis of the FLS2 and FLS3 receptors revealed domains of the proteins responsible for PAMP detection and for the different ROS response initiated by flgII-28/FLS3 as compared to flg22/FLS2. In addition, in vitro kinase assays and point mutations analysis identified FLS2 and FLS3 domains required for kinase activity and ATP binding. In research activities on tomato BSK830, we found that it interacts with PRRs and with the co-receptor SERK3A and PAMP treatment affects part of these interactions. CRISPR/Cas9 bsk830 mutant plants displayed enhanced pathogen susceptibility and reduced ROS production upon PAMP treatment. In addition, BSK830 interacted with 8 Xanthomonastype III secreted effectors. Follow up analysis revealed that among these effectors XopAE is part of an operon, is translocated into plant cells, and displays E3 ubiquitinligase activity. Our investigation was also extended to other Arabidopsis and tomato BSK family members. Arabidopsis BSK5 localized to the plant cell periphery, interacted with receptor-like kinases, and it was phosphorylatedin vitro by the PEPR1 and EFRPRRs. bsk5 mutant plants displayed enhanced susceptibility to pathogens and were impaired in several, but not all, PAMP-induced responses. Conversely, BSK5 overexpression conferred enhanced disease resistance and caused stronger PTI responses. Genetic complementation suggested that proper localization, kinase activity, and phosphorylation by PRRs are critical for BSK5 function. BSK7 and BSK8 specifically interacted with the FLS2 PRR, their respective mutant plants were more susceptible to B. cinereaand displayed reduced flg22-induced responses. The tomato BSK Mai1 was found to interact with the M3KMAPKKK, which is involved in activation of cell death associated with effector-triggered immunity. Silencing of Mai1 in N. benthamianaplants compromised cell death induced by a specific class of immune receptors. In addition, co-expression of Mai1 and M3Kin leaves enhanced MAPKphosphorylation and cell death, suggesting that Mai1 acts as a molecular link between pathogen recognition and MAPK signaling. Finally, We identified the PP2C phosphatase Pic1 that acts as a negative regulator of PTI by interacting with and dephosphorylating the receptor-like cytoplasmickinase Pti1, which is a positive regulator of plant immunity. The results of this investigation shed new light on the molecular characteristics and interactions of components of the immune system of crop plants providing new knowledge and tools for development of novel strategies for disease control.
APA, Harvard, Vancouver, ISO, and other styles
8

Allen, Robert J., and Ali Sadeghi. PAP Flap. Touch Surgery Simulations, March 2015. http://dx.doi.org/10.18556/touchsurgery/2015.s0044.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Conner, M. L. PAMS photo image retrieval prototype alternatives analysis. Office of Scientific and Technical Information (OSTI), April 1996. http://dx.doi.org/10.2172/10154323.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

O'Leary, Timothy J. Automated Rescreening of Pap Smears. Fort Belvoir, VA: Defense Technical Information Center, January 1996. http://dx.doi.org/10.21236/ada332970.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'PAMP' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography