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1
Brady, C. L., S. N. Venter, I. Cleenwerck, et al. "Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov." INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 59, no. 9 (2009): 2339–45. http://dx.doi.org/10.1099/ijs.0.009241-0.
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Brady, Carrie L., Ilse Cleenwerck, Lorinda van der Westhuizen, Stephanus N. Venter, Teresa A. Coutinho, and Paul De Vos. "Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus." International Journal of Systematic and Evolutionary Microbiology 62, Pt_7 (2012): 1457–64. http://dx.doi.org/10.1099/ijs.0.032615-0.
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Several Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from Eucalyptus seedlings showing symptoms of bacterial blight and dieback in Colombia, Rwanda and South Africa. Partial 16S rRNA gene sequencing, together with partial gyrB sequencing, placed the isolates in the genus Pantoea and indicated that they constituted three novel species. Multilocus sequence analysis (MLSA) based on partial sequences of gyrB, rpoB, infB and atpD revealed Pantoea dispersa , Pantoea eucrina and Pantoea cypripedii as their closest phylogenetic relatives. DNA–DNA hybridization studies confirmed the classification of the new isolates as three novel species and phenotypic tests allowed them to be differentiated from their closest phylogenetic neighbours. The names Pantoea rodasii sp. nov. [type strain LMG 26273T = BD 943T (deposited with the Plant Pathogenic and Plant Protecting Bacteria Collection, South Africa) = BCC 581T (deposited with the Bacterial Culture Collection, Forestry and Agricultural Institute, South Africa)], Pantoea rwandensis sp. nov. (type strain LMG 26275T = BD 944T = BCC 571T) and Pantoea wallisii sp. nov. (type strain LMG 26277T = BD 946T = BCC 682T) are proposed.
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Kageyama, B., M. Nakae, S. Yagi, and T. Sonoyama. "Pantoea punctata sp. nov., Pantoea citrea sp. nov., and Pantoea terrea sp. nov. Isolated from Fruit and Soil Samples." International Journal of Systematic Bacteriology 42, no. 2 (1992): 203–10. http://dx.doi.org/10.1099/00207713-42-2-203.
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Popp, Alexandra, Ilse Cleenwerck, Carol Iversen, Paul De Vos, and Roger Stephan. "Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment." International Journal of Systematic and Evolutionary Microbiology 60, no. 12 (2010): 2786–92. http://dx.doi.org/10.1099/ijs.0.019430-0.
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Five Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. Biochemical tests and partial rpoB gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family Enterobacteriaceae, closely related to several species of the genera Pantoea and Erwinia, which indicated a phylogenetic position within the genus Pantoea or the genus Erwinia. Multilocus sequence analysis of concatenated partial atpD, gyrB, infB and rpoB gene sequences of two of the isolates suggested that they represented two novel species of the genus Pantoea, phylogenetically related most closely to Pantoea septica. The five isolates had general characteristics consistent with those of the genus Pantoea, and DNA–DNA hybridizations between two representatives and the type strains of their phylogenetically closest relatives based on comparative 16S rRNA gene sequence analysis showed that the isolates represented two novel genospecies. These two genospecies could be differentiated from each other based on fermentation of galacturonate, sorbitol and potassium 5-ketogluconate. They could be differentiated from phylogenetically related Pantoea species based on their ability to ferment lactose and to utilize β-gentiobiose and raffinose, their inability to ferment or utilize d-arabitol, and their inability to produce indole. On the basis of the results obtained, the five isolates are considered to represent two novel species of the genus Pantoea, for which the names Pantoea gaviniae sp. nov. (type strain A18/07T =LMG 25382T =DSM 22758T) and Pantoea calida sp. nov. (type strain 1400/07T =LMG 25383T =DSM 22759T) are proposed.
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Rijavec, Tomaž, Aleš Lapanje, Marina Dermastia, and Maja Rupnik. "Isolation of bacterial endophytes from germinated maize kernels." Canadian Journal of Microbiology 53, no. 6 (2007): 802–8. http://dx.doi.org/10.1139/w07-048.
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The germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. Bacterial strains identified by partial 16S rRNA gene sequencing as Pantoea sp., Microbacterium sp., Frigoribacterium sp., Bacillus sp., Paenibacillus sp., and Sphingomonas sp. were isolated from kernels of 4 different maize cultivars. Genus Pantoea was associated with a specific maize cultivar. The kernels of this cultivar were often overgrown with the fungus Lecanicillium aphanocladii ; however, those exhibiting Pantoea growth were never colonized with it. Furthermore, the isolated bacterium strain inhibited fungal growth in vitro.
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Kato Tanaka, Yuko, Nobuhiro Horie, Kaoru Mochida, Yoshihiro Yoshida, Eri Okugawa, and Fumio Nanjo. "Pantoea theicola sp. nov., isolated from black tea." International Journal of Systematic and Evolutionary Microbiology 65, Pt_10 (2015): 3313–19. http://dx.doi.org/10.1099/ijsem.0.000412.
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A Gram-negative, facultatively anaerobic strain was isolated from black tea. On the basis of 16S rRNA gene sequence similarity comparisons, strain QC88-366T was grouped into the genus Pantoea, being related most closely to the type strains of Pantoea gaviniae (98.5 %) and Pantoea calida (98.3 %); sequence similarities were ≤ 97.0 % to the type strains of other species of the genus Pantoea. Multilocus sequence analysis based on partial sequences of the gyrB, rpoB, infB and atpD genes also revealed P. gaviniae and P. calida as the closest phylogenetic relatives. The fatty acid profile showed the major fatty acids of strain QC88-366T were C16 : 0, C16 : 1 and C18 : 1, the same as those of its closest related species. However, the ratio of C16 : 1, C17 : 0 cyclo, C18 : 1 and C18 : 2 differed slightly compared with those of the related neighbours. In addition, the results of physiological and biochemical tests also allowed the phenotypic differentiation of strain QC88-366T from its closest phylogenetic neighbours. The G+C content of the DNA was 57.2 mol%. Strain QC88-366T therefore represents a novel species of the genus Pantoea, for which the name Pantoea theicola sp. nov. is proposed. The type strain is QC88-366T ( = DSM 29212T = NBRC 110557T).
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Porotikova, Elena, Natalia Brusnova, Andrei Sushchenko, Galina Kolganikhina, and Svetlana Vinogradova. "Bacteria Pseudomonas sp. and Pantoea sp. Are the New Etiological Agents of Diseases on Forest Trees." Plants 14, no. 4 (2025): 563. https://doi.org/10.3390/plants14040563.
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Forest trees significantly affect human life. The spread of pathogens, including bacterial ones, poses a serious threat to their health. Despite this, however, the species composition and distribution of pathogenic bacteria, as well as the etiology of common diseases affecting forest trees, remain virtually unstudied. In this study, we, for the first time, describe different species of Pseudomonas and Pantoea as new etiological agents associated with the symptoms of leaf spotting and wood darkening on Acer tataricum L., Fraxinus pennsylvanica L., Ulmus minor Mill. Ulmus laevis Pallas. and Populus tremula L. For the identification of bacteria species, we used an integrated approach based on the characterization of their morphology, biochemistry, physiology and genetics. Phylogenetic analysis was performed using multilocus typing for five genes for Pseudomonas and six genes for Pantoea. Leaf spotting on A. tataricum, F. pennsylvanica, U. minor and U. laevis was shown to be caused by Pseudomonas cerasi, Pseudomonas congelans, Pseudomonas graminis, Pseudomonas syringae and Pantoea agglomerans both in monoinfection and coinfection. Wood darkening in U. minor U. laevis and P. tremula was found to be associated with the presence of Pantoea sp. and P. agglomerans. The coinfection of forest trees with bacteria of the genera Pseudomonas and Pantoea indicates a complex mechanism of interaction between the two populations, which will be the subject of future studies.
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Brady, Carrie L., Ilse Cleenwerck, Stephanus N. Venter, Katrien Engelbeen, Paul De Vos, and Teresa A. Coutinho. "Emended description of the genus Pantoea, description of four species from human clinical samples, Pantoea septica sp. nov., Pantoea eucrina sp. nov., Pantoea brenneri sp. nov. and Pantoea conspicua sp. nov., and transfer of Pectobacterium cypripedii (Hori 1911) Brenner et al. 1973 emend. Hauben et al. 1998 to the genus as Pantoea cypripedii comb. nov." International Journal of Systematic and Evolutionary Microbiology 60, no. 10 (2010): 2430–40. http://dx.doi.org/10.1099/ijs.0.017301-0.
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Bacterial strains belonging to DNA hybridization groups (HG) II, IV and V, in the Erwinia herbicola–Enterobacter agglomerans complex, of Brenner et al. [Int J Syst Bacteriol 34 (1984), 45–55] were suggested previously to belong to the genus Pantoea, but have never been formally described and classified. Additionally, it has been shown in several studies that Pectobacterium cypripedii is more closely related to species of Pantoea than to those of Pectobacterium. In this study, the phylogenetic positions of Brenner's DNA HG II, IV and V and Pectobacterium cypripedii were re-examined by both 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) based on the gyrB, rpoB, atpD and infB genes. The analyses revealed that DNA HG II, IV and V and Pectobacterium cypripedii form five separate branches within the genus Pantoea (strains from HG V were split into two branches). DNA–DNA hybridization data further confirmed that DNA HG II, IV and V constitute four separate species. Pectobacterium cypripedii was shown to be a close phylogenetic relative of Pantoea dispersa and DNA HG IV by both 16S rRNA gene sequence and MLSA analyses. Biochemical analyses performed on strains from DNA HG II, IV and V and Pectobacterium cypripedii confirmed their taxonomic position within the genus Pantoea and revealed phenotypic characteristics that allow the differentiation of these species from each other and from their closest phylogenetic neighbours. It is proposed to emend the description of the genus Pantoea and to describe Pantoea septica sp. nov. for DNA HG II (type strain LMG 5345T =BD 874T =CDC 3123-70T), Pantoea eucrina sp. nov. for DNA HG IV (type strain LMG 2781T =BD 872T =CDC 1741-71T =LMG 5346T), Pantoea brenneri sp. nov. for strains of DNA HG V excluding LMG 24534 (type strain LMG 5343T =BD 873T =CDC 3482-71T) and Pantoea conspicua sp. nov. for the remaining strain of DNA HG V (type strain LMG 24534T =BD 805T =CDC 3527-71T) and to transfer Pectobacterium cypripedii to the genus as Pantoea cypripedii comb. nov. (type strain LMG 2657T =ATCC 29267T =DSM 3873T =LMG 2655T).
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Matsui, Masayoshi, Takanori Tanino, Misato Ito, et al. "Growth Properties and Sensitivities to Various Bactericidal Methods of Cold-Tolerant Microorganisms Isolated from Packed Tofu." Agronomy 12, no. 2 (2022): 233. http://dx.doi.org/10.3390/agronomy12020233.
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Disinfection and sterilization against cold-tolerant microorganisms are very important for enhancing food safety and hygiene management under refrigeration conditions. We isolated Exiguobacterium sp. and Pantoea sp. from an immersion solution of packed tofu and evaluated their growth properties and sensitivities to various bactericidal methods. These microorganisms were classified as psychrotrophic bacteria, according to their ability to grow at 4 °C, with an optimum growth temperature of 30 °C. Exiguobacterium sp. and Pantoea sp. were more sensitive to heat and sodium hypochlorite than Escherichia coli (E. coli). Exiguobacterium sp. was sensitive to acetic acid and citric acid, while Pantoea sp. was relatively insensitive and E. coli was insensitive to these organic acids. All microorganisms used in this study were resistant to sodium hydroxide solution (pH 8.00). Exiguobacterium sp. showed resistance to ozonated water and UV; therefore, particular attention should be paid to the contamination of this microorganism in food factories. Both Exiguobacterium sp. and Pantoea sp. were inactivated by pulsed electric field (PEF) treatment that did not cause lethal damage to E. coli. The results obtained in this study suggest that the bactericidal methods targeting the fragile cell membrane with high permeability are effective for the inactivation of cold-tolerant microorganisms.
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Jasso-Arreola, Yesenia, J. Antonio Ibarra, Flor de Fátima Rosas-Cárdenas, and Paulina Estrada-de los Santos. "Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of Coffea arabica L." Plants 14, no. 7 (2025): 1084. https://doi.org/10.3390/plants14071084.
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Given the challenges of climate change, effective adaptation strategies for crops like coffee are crucial. This study evaluated twelve 1-aminocyclopropane-1-carboxylate deaminase-producing bacterial strains selectively isolated from the rhizosphere of Coffea arabica L. cv. Costa Rica 95 in a plantation located in Veracruz, Mexico, focusing on their potential to enhance drought resistance. The strains, representing seven genera from the Gamma-proteobacteria and Bacteroidota groups, were characterized for growth-promoting traits, including ACC deaminase activity, indole-3-acetic acid (IAA) synthesis, phosphates solubilization, siderophore production, and nitrogen fixation. Strains of the genus Pantoea exhibited higher ACC deaminase activity, phosphate solubilization, and IAA synthesis, while others, such as Sphingobacterium and Chryseobacterium, showed limited plant growth-promoting traits. A pot experiment was conducted with coffee plants subjected to either full irrigation (soil with 85% volumetric water content) or drought (soil with 55% volumetric water content) conditions, along with inoculation with the isolated strains. Plants inoculated with Pantoea sp. RCa62 demonstrated improved growth metrics and physiological traits under drought, including higher leaf area, relative water content (RWC), biomass, and root development compared to uninoculated controls. Similar results were observed with Serratia sp. RCa28 and Pantoea sp. RCa31 under full irrigation conditions. Pantoea sp. RCa62 exhibited superior root development under stress, contributing to overall plant development. Proline accumulation was significantly higher in drought-stressed, non-inoculated plants compared to those inoculated with Pantoea sp. RCa62. This research highlights the potential of Pantoea sp. RCa62 to enhance coffee plant resilience to drought and underscores the need for field application and further validation of these bioinoculants in sustainable agricultural practices.
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Brady, Carrie L., Teresa Goszczynska, Stephanus N. Venter, et al. "Pantoea allii sp. nov., isolated from onion plants and seed." International Journal of Systematic and Evolutionary Microbiology 61, no. 4 (2011): 932–37. http://dx.doi.org/10.1099/ijs.0.022921-0.
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Eight yellow-pigmented, Gram-negative, rod-shaped, oxidase-negative, motile, facultatively anaerobic bacteria were isolated from onion seed in South Africa and from an onion plant exhibiting centre rot symptoms in the USA. The isolates were assigned to the genus Pantoea on the basis of phenotypic and biochemical tests. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA), based on gyrB, rpoB, infB and atpD sequences, confirmed the allocation of the isolates to the genus Pantoea. MLSA further indicated that the isolates represented a novel species, which was phylogenetically most closely related to Pantoea ananatis and Pantoea stewartii. Amplified fragment length polymorphism analysis also placed the isolates into a cluster separate from P. ananatis and P. stewartii. Compared with type strains of species of the genus Pantoea that showed >97 % 16S rRNA gene sequence similarity with strain BD 390T, the isolates exhibited 11–55 % whole-genome DNA–DNA relatedness, which confirmed the classification of the isolates in a novel species. The most useful phenotypic characteristics for the differentiation of the isolates from their closest phylogenetic neighbours are production of acid from amygdalin and utilization of adonitol and sorbitol. A novel species, Pantoea allii sp. nov., is proposed, with type strain BD 390T ( = LMG 24248T).
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Zakry, FAA, P. Ammal, M. Malahubban, A. R. Faridah, and A. H. M. Umar. "Selecting the most effective plant growth-promoting bacteria from oil palm (Elaeis guineensis Jacq) roots." Journal of the Bangladesh Agricultural University 17, no. 3 (2019): 344–48. http://dx.doi.org/10.3329/jbau.v17i3.43208.
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A total of 30 bacterial isolates were isolated from soil, rhizoplane, and internal tissue of oil palm roots. The isolates were qualitatively tested for their potential to fix N2, solubilize inorganic P and K, and produce phytohormone indole-3-acetic acid. Of the 30 isolates, six isolates were able to exhibit multiple beneficial traits. All six isolates were then identified based on fatty acid methyl esters profile as Escherichia coli strain EX2, Serratia sp. strain EN1, Pantoea ananatis strain EN3, Bacillus sp. strain EN5, Pantoea ananatis strain EN8 and Pantoea sp. strain EN9. Subsequently, all shortlisted isolates were evaluated for plant growth-promoting potential by using shallot as a test plant. The plant test showed no significant difference (p>0.05) between inoculated and uninoculated plants except for Pantoea sp. strain EN9 inoculation which increased significantly (p<0.05) total root length over uninoculated control. Host specificity and IAA capacity of the isolates may be among the important factors affecting their effectiveness in plant growth promotion.
 J Bangladesh Agril Univ 17(3): 344–348, 2019
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Itkina, Daria, and Aliya Suleimanova. "Phytate-hydrolyzing rhizobacteria: abiotic stress tolerance and antimicrobial activity." E3S Web of Conferences 222 (2020): 02055. http://dx.doi.org/10.1051/e3sconf/202022202055.
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Phytate-hydrolyzing bacteria Pantoea sp. 3.1, 3.2, 3.5.2, 3.6.1 and Bacillus ginsengihumi M2.11 were previously isolated from the soil samples of the Republic of Tatarstan. The effect of cultivation conditions on the growth dynamics as well as antimicrobial activity was determined. All four Pantoea strains showed optimum growth at 26 ºC and 28 ºC and pH 6.0-7.0. The optimum conditions for the growth of B. ginsengihumi M2.11 strain was determined to be 26 ºC, 28 ºC and 37 ºC and alkaline pH 7 and 8. Salt concentration in the range of 0 to 1000 mM did not significantly affect the growth of the strains. Antagonistic activity of Pantoea sp. 3.5.2 was studied against phytopathogenic micromycetes, identified as Alternaria alternata and Bipolaris sorokiniana. In the presence of bacterial isolate growth of A. alternata was inhibited by 57% and growth of B. sorokiniana – by 85%. Minor growth inhibition by Pantoea sp. 3.5.2 of gram-negative bacteria from Enterobacteriaceae family was observed. The presence of fungicidal activity in the Pantoea strain together with its ability to hydrolyze soil phytates and overcome abiotic stress factors in soil can possibly serve as the basis for the new fungicide of microbial origin.
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Tín, Nguyễn Chánh, Nguyễn Vĩnh Thái, Trương Thị Anh Thư, Trần Võ Khánh Toàn, and Trần Thị Giang. "EFFICIENCY OF NITROGEN-FIXING BACTERIA BRADYRHIZOBIUM SP. AND PHOSPHATE-SOLUBILIZING BACTERIA PSEUDOMONAS SP., PANTOEA SP. ON THE GROWTH OF MAIZE [ZEA MAYS L.]." TNU Journal of Science and Technology 228, no. 05 (2023): 455–62. http://dx.doi.org/10.34238/tnu-jst.7220.
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Nghiên cứu được thực hiện nhằm khảo sát tác động của các dòng vi khuẩn cố định đạm Bradyrhizobium sp. TTB2.1 và hòa tan lân Pseudomonas sp. BKĐ2.1, Pseudomonas sp. TTB4.4, Pantoea sp. TTB4.1 lên sự sinh trưởng, phát triển của cây bắp trong điều kiện ngoài đồng. Kết quả cho thấy năng suất ở các nghiệm thức tưới vi khuẩn hòa tan lân Pantoea sp. TTB4.1 đạt 25,64 tấn/ha (vụ 1) và 27,22 tấn/ha (vụ 2), Pseudomonas sp. BKĐ2.1 cho kết quả đạt 25,71 tấn/ha (vụ 1) và 26,98 tấn/ha (vụ 2). Các chỉ tiêu về chiều cao cây, chiều dài trái, chiều dài lá, số trái trên cây và hàm lượng chlorophyll trong lá ở các nghiệm thức tưới vi khuẩn cao hơn đối chứng âm. Các chỉ tiêu về hàm lượng đạm, lân hòa tan, mật số vi sinh vật trong đất có sự thay đổi giữa trước khi gieo hạt và sau thu hoạch. Hai dòng vi khuẩn hòa tan lân Pseudomonas sp. BKĐ2.1 và Pantoea sp. TTB4.1 có tác động lên sự sinh trưởng và phát triển của cây bắp tốt nhất.
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Hernández, Ionel, Cecilia Taulé, Reneé Pérez-Pérez, et al. "Endophytic Seed-Associated Bacteria as Plant Growth Promoters of Cuban Rice (Oryza sativa L.)." Microorganisms 11, no. 9 (2023): 2317. http://dx.doi.org/10.3390/microorganisms11092317.
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Cuban rice cultivars INCA LP-5 and INCA LP-7 are widely distributed in Cuba and Caribbean countries. Although there are studies about rhizospheric bacteria associated with these cultivars, there are no reports about their seed-associated bacteria. This study aimed to isolate endophytic bacteria from rice seeds and select those with the greatest plant growth-promoting traits. A total of nineteen bacterial strains from the genera Pantoea, Bacillus, Paenibacillus, and Pseudomonas were isolated from the husk and endosperm of rice seeds. The strains Pantoea sp. S5-1, Pseudomonas sp. S5-38, and Pseudomonas sp. S7-1 were classified as the most promissory to increase rice growth as they demonstrated the presence of multiple plant growth-promoting traits such as the production of auxins, phosphate, and potassium solubilization, the production of siderophores, and the inhibition of the phytopathogen Pyricularia oryzae. The inoculation of strains of Pantoea sp. and Pseudomonas spp. in rice improves the height, root length, fresh weight, and dry weight of the shoot and root after 21 days post-inoculation in hydroponic assays. This study constitutes the first report on Cuban rice cultivars about the presence of endophytes in seeds and their potential to promote seedling growth. Pantoea sp. S5-1, Pseudomonas sp. S5-38, and Pseudomonas sp. S7-1 were selected as the more promising strains for the development of bio-stimulators or bio-inoculants for Cuban rice crops.
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Mayanglambam, Thoibi, Pushpendra Kumar Sharma, D. K. Singh, et al. "Biodegradation of quinalphos by gram negative bacteria Pantoea agglomerans and Acinetobacter sp. dcm5A." Environment Conservation Journal 24, no. 2 (2023): 373–79. http://dx.doi.org/10.36953/ecj.24132635.
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Five bacterial strains for biodegradation study were initially isolated from quinalphos exposed soil from cotton field of Indian Agricultural Research Institute, India by Serial dilution and enrichment technique. Amongst them, two strains Pantoea agglomerans and Acinetobacter sp. dcm5A were exposed to different concentrations (50-250 ppm, parts per million) of quinalphos to evaluate their tolerance and the optimum concentration of quinalphos supported growth. Growth kinetics of the isolates was studied by means of optical density of the culture media (150 rpm, rotation per minute) at 30oC for 15 days. The optimal concentration of quinalphos for the growth of Pantoea agglomerans was 100 ppm, while for Acinetobacter sp. dcm5A was 200 ppm. Bacterial strains Pantoea agglomerans and Acinetobacter sp. dcm5A degraded quinalphos 60% and 79.7% respectively in 7 days incubation and 100% degradation by both strains was observed after 13 days of incubation.
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Prakash, Om, Yogesh Nimonkar, Ankita Vaishampayan, et al. "Pantoea intestinalis sp. nov., isolated from the human gut." International Journal of Systematic and Evolutionary Microbiology 65, Pt_10 (2015): 3352–58. http://dx.doi.org/10.1099/ijsem.0.000419.
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A novel bacterial strain, 29Y89BT, was isolated from a faecal sample of a healthy human subject. Cells were Gram-stain-negative, motile, non-spore-forming and rod-shaped. Strain 29Y89BT formed cream-coloured colonies 2 mm in diameter on trypticase soy agar and showed optimum growth at 35 °C. Strain 29Y89BT showed highest 16S rRNA gene sequence similarity to Pantoea gaviniae A18/07T (98.4 %) followed by Pantoea calida 1400/07T (97.2 %). Multi-locus sequence analysis using atpD (ATP synthase β subunit), gyrB (DNA gyrase), infB (initiation translation factor 2) and rpoB (RNA polymerase β subunit) genes also supported the result of 16S rRNA gene sequence based phylogeny. Strain 29Y89BT showed 62 and 40.7 % DNA–DNA relatedness with P. calida DSM 22759T and P. gaviniae DSM 22758T. Strain 29Y89BT contained C17 : 0 cyclo, C19 : 0 cyclo ω8c, C16 : 0, C14 : 0 and C12 : 0 as predominant fatty acids. In addition, strain 29Y89BT showed physiological and phenotypic differences from its closest relatives P. gaviniae DSM 22758T and P. calida DSM 22759T. The polar lipid profile mainly comprised phospholipids. The DNA G+C content was 59.1 mol%. Thus, based on the findings of the current study, strain 29Y89BT showed clear delineations from its closest relatives P. gaviniae DSM 22758T and P. calida DSM 22759T, and is thus considered to represent a novel species of the genus Pantoea, for which the name Pantoea intestinalis sp. nov. is proposed. The type strain is 29Y89BT ( = DSM 28113T = MCC 2554T).
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Nam, Young Ho, Ahyoung Choi, and Eu Jin Chung. "Antibacterial Activities of Strain Pantoea sp. S3W-11 and Optimization of Culture Conditions." KSBB Journal 35, no. 1 (2020): 64–71. http://dx.doi.org/10.7841/ksbbj.2020.35.1.64.
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Yao, Bo, Rong Huang, Zhenfen Zhang, and Shangli Shi. "Diverse Virulence Attributes of Pantoea alfalfae sp. nov. CQ10 Responsible for Bacterial Leaf Blight in Alfalfa Revealed by Genomic Analysis." International Journal of Molecular Sciences 24, no. 9 (2023): 8138. http://dx.doi.org/10.3390/ijms24098138.
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Alfalfa is widely grown worldwide for its excellent nutritional value. Pantoea species living in alfalfa seeds can easily spread over great distances with frequent trade. However, the pathogenic properties of this dangerous hitchhiker on alfalfa have not been evaluated. Here, we identified the taxonomic status of Pantoea strain CQ10 isolated from the interior of alfalfa seeds based on the whole genome sequence. The diverse virulence attributes of strain CQ10 during host infection were characterized through pathogenicity assays and functional and genomic analyses. We report that strain CQ10 belongs to a novel species in the genus Pantoea, which was phylogenetically close to Pantoea vagans and Pantoea agglomerans. Strain CQ10 caused bacterial leaf blight of alfalfa after inoculation from the roots. We found that strain CQ10 possesses a large number of pathogenic genes involved in shaping the virulence properties during bacteria–host interactions, including motility, biofilm, type VI secretion system, and nutrient acquisition. Compared with P. vagans and P. agglomerans, the unique virulence factors of strain CQ10 were mainly involved in motility and biofilm, which were confirmed by in vitro experiments. Taken together, our results suggest that strain CQ10 is the first Pantoea species to infect alfalfa, and it possesses diverse virulence attributes among which motility and biofilm may be the best weapons.
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Gueule, Dominique, Gérard Fourny, Elisabeth Ageron, et al. "Pantoea coffeiphila sp. nov., cause of the ‘potato taste’ of Arabica coffee from the African Great Lakes region." International Journal of Systematic and Evolutionary Microbiology 65, Pt_1 (2015): 23–29. http://dx.doi.org/10.1099/ijs.0.063545-0.
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Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea . By DNA–DNA hybridization, the isolates constituted a genomic species with less than 17 % relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea . Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp.nov. and its type strain is Ca04T ( = CIP 110718T = DSM 28482T).
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Epova, Ekaterina, Svetlana Petrova, Elena Trubnikova, and Maria Danilova. "Epiphytic bet-mannanase producing bacterial strains." BIO Web of Conferences 37 (2021): 00125. http://dx.doi.org/10.1051/bioconf/20213700125.
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Dry remains of the herbal species of the plantain (Plantago major), the wormwood (Artemisia vulgaris) and the reed grass (Calamagrostis acutiflora) were used as a natural source for isolation of β- mannanase producing strains. They were isolated by using the carob gum as a single source of carbon and energy. Each chosen plant species was found to be colonized with a single dominant epiphytic group of microorganism, although the plants had been collected in the same location. Bacillus circulans was only found in P. major, Bacillus subtilis on A. vulgaris, whereas Pantoea sp. was found in C. acutiflora. Identification of the taxonomy affiliation of the isolated β-mannanase producers allowed using the formerly proposed primers for PCR cloning of β-mannanase genes previously occurred in the respective bacterial species. This approach let us cloning 330 bp fragment of β-mannanase genes from B. circulans and B. subtilis and 1000 bp fragment of β-mannanase gene from Pantoea sp. Testing the enzymatic activity of the isolated strains by staining the carob gum hydrolysis zones on the plates with Congo Red was carried out. As a result, the maximum activity was found in Pantoea sp.
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Bible, Amber N., Mang Chang, and Jennifer L. Morrell-Falvey. "Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343." PLOS ONE 16, no. 7 (2021): e0248607. http://dx.doi.org/10.1371/journal.pone.0248607.
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Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere of Populus deltoides, forms robust biofilms along the root surfaces of Populus and possesses plant growth-promoting characteristics. In this work, we identified three diguanylate cyclases in the plant-associated microbe Pantoea sp. YR343 that are expressed in the presence of plant roots. One of these diguanylate cyclases, DGC2884, localizes to discrete sites in the cells and its overexpression results in reduced motility and increased EPS production and biofilm formation. We performed a genetic screen by expressing this diguanylate cyclase from an inducible promoter in order to identify candidate gene products that may be involved in root colonization by Pantoea sp. YR343. Further, we demonstrate the importance of other domains in DGC2884 to its activity, which in combination with the genes identified by transposon mutagenesis, may yield insights into the mechanisms of plant association as well as the activity and regulation of homologous enzymes in medically and agriculturally relevant microbes.
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Kim, M. K., J. S. Ryu, Y. H. Lee, and H. D. Yun. "First Report of Pantoea sp. Induced Soft Rot Disease of Pleurotus eryngii in Korea." Plant Disease 91, no. 1 (2007): 109. http://dx.doi.org/10.1094/pd-91-0109a.
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The king oyster mushroom, Pleurotus eryngii, has become a popular crop because of its unique flavor and texture and is cultivated in many areas in Korea. In 2003, symptoms of water-soaked lesions and soft rot in the stipes and pileus of cultivated P. eryngii was observed in Jinju, Korea. Diseased tissue was plated on nutrient media. Dominate colonies were yellow, convex, circular with smooth margins, and had a shiny texture. Computer analysis of the data gathered, using the API kit (50CHE, bioMérieux, Marcy-l'Etoile, France), showed that the strain belongs to the Enterobacteriaceae. Although the API system did not give an exact identification, the metabolic profile of the bacterial strain closely resembled the database profile of Pantoea sp. (positive for acid production from the fermentation of d-fructose, d-galactose, d-glucose, d-trehalose, and d-ribose and negative for oxidase, urease, pectate, and thiosulfate). The 16S rDNA sequence of the bacterium was determined (GenBank Accession No. AY530796). When compared with those in GenBank, the bacterium was determined to belong to the Enterobacteriaceae family of the Gammaproteobacteria, and the highest degree of sequence similarity was found to be with Pantoea ananatis strain BD 588 (97.4%) and Pantoea ananatis strain Pna 97-1 (97.3%). In the phylogenetic tree, the bacterium clearly related to the Pantoea lineage, as evidenced by the high bootstrap value. A BLAST search with 16S rDNA sequence of the bacterium supported the API results that the isolate belongs to a species of Pantoea. Pathogenicity tests of this new Pantoea isolate were carried out with bacterial suspensions (approximately 1 × 106 CFU/ml) that were grown for 24 h in Luria-Bertani broth cultures. These were used to inoculate directly on the mycelia of P. eryngii that had been cultivated for 35 days in a plastic bottle. The water and broth were also inoculated to another set of bottles as a control experiment. Inoculated bottles were incubated in a cultivation room at 16 to 17°C with relative humidity between 80 and 95%. Early symptoms of the disease included a dark brown water drop that developed on hypha and primordium of the mushrooms after 5 to 7 days. After 13 days, water-soaked lesions developed on the stipes and pileus, and the normal growth of the mushrooms was inhibited. An offensive odor then developed along with a severe soft rot that was similar to the disease symptoms observed under natural conditions. Mushrooms in control bottles did not develop symptoms. Koch's postulates were fulfilled by isolating bacteria from typical lesions from inoculated mushrooms that were identical to the inoculated strain in colony morphology and biochemical characteristics. Pantoea ananatis was first reported as a pathogen of pineapple fruit causing brown rot (3). Several bacterial diseases, such as brown blotch on cultivated mushrooms by Pseudomonas tolaasii (2) and bacterial soft rot on winter mushroom by Erwinia carotovora subsp. Carotovora, causing severe damage to mushrooms are known (1). However, no Pantoea sp. induced disease of edible mushroom has been previously reported. To our knowledge, this is the first report of soft rot disease on P. eryngii caused by Pantoea sp. References: (1) H. Okamoto et al. Ann. Phytopathol. Soc. Jpn. 65:460. 1999. (2) S. G. Paine. Ann. Appl. Biol. 5:206. 1919. (3) F. B. Serrano. Philipp. J. Sci. 36:271, 1928.
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Akhter, Adnan, Waqas Bin Nisar, Muhammad Taqqi Abbas, et al. "CHARACTERIZATION AND EVALUATION OF ANTIFUNGAL POTENTIAL OF BACTERIAL ENDOPHYTES OF RICE AGAINST FUSARIUM MONILIFORME AND RHIZOCTONIA SOLANI." Agricultural Sciences Journal 4, no. 2 (2022): 13–27. http://dx.doi.org/10.56520/asj.v4i2.149.
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Rice (Oryza sativa L.) is one of the most important staple food crop of Pakistan as well as in the whole world. A number of phyto-pathogens including bacteria, fungi, nematodes and viruses along with abiotic stresses such as drought, salinity, nutrient deficiency and etc. have a detrimental effect on rice yield. In order to overcome the attack of pathogenic microbes, use of endophytes as bio-control agent is an attractive way to minimize the use of toxic agrochemicals. In this study, we screened seven strains of bacterial endophytes from the rhizosphere of rice plants. A colony PCR was performed using universal primers to characterize the isolated bacterial endophytes followed by sequence analysis. On the basis of molecular characterization, endophytes were identified as Pantoea sp., Burkholderiasp Bacillus megaterium, Pseudomonas flourescens, Lysinibacillus fusiformis, Delftia sp., and Acinetobacter baumnii. Additionally, endophytes were assayed for In vitro effects against Fusarium moniliforme and Rhizoctonia solani causing bakanae and sheath blight disease of rice, respectively. Pantoea sp., Burkholderia sp., Bacillus megaterium and Delftiasp moderately suppressed Fusarium moniliforme but Pseudomonas flourescens, Lysinibacillus fusiformis both showcased a strong inhibiting activity against Fusarium moniliforme. On the other hand, Pantoea sp., Burkholderia sp., Bacillus megaterium, Pseudomonas flourescens and Lysinibacillus fusiformis had a strong inhibitory effect against Rhizoctonia solani. The isolated endophytic bacteria were also found to be good producers of phyto-hormones such as hydrogen cyanide (HCN) and catalase. On the basis of our results, we conclude that the endophytic bacteria from rice rhizosphere possess antifungal activity against economic important pathogenic fungi.
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Jaber, Amany Shakeir, and Faez Khalaf Abdulmuhsen. "Detection of some virulence factors of Pantoea species isolated from urinary tract infections in the Iraqi population." Journal of Applied and Natural Science 15, no. 4 (2023): 1720–26. http://dx.doi.org/10.31018/jans.v15i4.4991.
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Pantoea is an opportunistic bacterium primarily involved in nosocomial infections, impacting newborns and immunocompromised patients. The study aimed to investigate the virulence factor of Pantoea in urinary tract infections in hospitals in Al-Nasiriyah City, Iraq. The outcome appeared that 22 isolates out of 100 urine samples had been confirmed as Pantoea species by VITEK- 2. RcsA and hrpA genes were detected using conventional Polymerase Chain Reactions (PCR) and specific primers. The distribution of rcsA and hrpA was 40.9% and 59.1% respectively. The Pantoea species were distributed according to age, gender, and ABO blood groups. The percentage was 72.3% for ages 40–70, and females recorded 54.5% more than males. Individuals with the A blood group had a higher percentage (50%) than other blood groups; there were two types of variations (Transversion and Transition) of the16S rRNA gene. It was determined through the sequence alignment, and the symmetry was 90% with the Italian isolate of Pantoea species LL92 (ID: KF202812.1). These variations in the Pantoea local Iraqi isolate were registered in NCBI (ID: OM971851).This bacterial genus recently descended from the species of Enterobacter sp.. The isolation of Pantoea sp.was from UTIs, while previously, in most studies, they were isolated from the surrounding environment.
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TURAEVA, B. "MICROFLORA AND PLANT PATHOGENIC FUNGI AFFECTING BACTERIA IN GRAPE PLANTATIONS IN UZBEKISTAN." SABRAO Journal of Breeding and Genetics 55, no. 6 (2023): 2037–51. http://dx.doi.org/10.54910/sabrao2023.55.6.17.
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In the presented study, isolation of bacterial strains, viz., Pantoea agglomerans, Priestia megaterium and phytopathogenic micromycetes that cause damage and eventually death of grape crops, came from a 10 to 15-year-old vine plantation. A Pantoea agglomerans gram-negative bacillus facultative and anaerobic bacterium strain achieved isolation from grape plants, with its morphological characteristics studied. Bacterial strains with antifungal activities against phytopathogenic micromycetes succeeded in their identification. Bacterial isolates collected from the vines underwent screening for their growth properties. It was apparent that Pantoea agglomerans actively grew wheat coleoptiles by 2.6 mm and maize coleoptiles by 2.3 mm compared with the control. Observable evidence also showed that sorghum coleoptile actively grew by 1.7 mm compared with the control treatment by 2.9 mm. The 26 Aspergillus sp., 23 Penicillium sp., 25 Fusarium sp., 30 Alternaria sp., and five Curvularia sp. phytopathogenic micromycetes belonging to the genus were notable. Bacterial strains isolated from the vine showed the highest antifungal activity against micromycetes belonging to the genus Penicillium and reduced the radius of phytopathogenic growth to 47–54 mm. Compared with micromycetes belonging to the genus Fusarium, it was also apparent that the pathogen reduced the growth radius to 27–35 mm. Isolation of phytopathogenic micromycetes from the vine allows early detection and prevention of grape diseases. Based on these studies, the identification of antifungal activity of the bacterial strains isolated from the vine and the presence of phytohormones in the culture fluid indicated that it is an essential and environmentally friendly biological tool in the cultivation of grapes for human consumption.
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Sugiani, Desy, Uni Urwaningsih, Septyan Andriyanto, and Angela Mariana Lusiastuti. "bakteri pada ikan gabus Channa striata, semah Tor spp., dan baung Hemibagrus sp.: identifikasi, virulensi, dan kerentanan terhadap beberapa antibiotik." Jurnal Riset Akuakultur 13, no. 4 (2019): 347. http://dx.doi.org/10.15578/jra.13.4.2018.347-356.
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Identifikasi mikroflora ikan air tawar adalah alat penting untuk evaluasi kualitas dan keamanan ikan untuk dipelihara dalam sistem budidaya intensif. Penelitian ini bertujuan untuk mendeteksi bakteri ikan air tawar terutama dari ikan gabus, semah, dan baung hasil tangkapan dan budidaya pada kolam. Bakteri yang terisolasi dari jaringan ginjal, hati, luka, dan otak ikan gabus (Channa striata), semah (Tor spp.), dan baung (Hemibagrus sp.) diidentifikasi dengan uji fisik dan biokimia, dan dilanjutkan dengan menggunakan API 20 E dan API 20 Strep. Uji virulensi untuk melihat kemampuan patogenisitas dari masing-masing isolat bakteri dilakukan dengan injeksi intra muskular suspensi bakteri 106 CFU mL-1 per ekor ikan. Ikan gabus dapat terinfeksi bakteri Pasteurella pneumotropica dan Aeromonas hydrophila, ikan semah rentan terhadap Enterococcus faecium, Pantoea spp., dan A. hydrophila, sedangkan ikan baung rentan terhadap infeksi Citrobacter freundii dan A. hydrophila. Bakteri Enterococcus faecium, Pantoea sp., dan A. hydrophila memiliki tingkat virulensi yang rendah antara 3,3%-23,4%; sedangkan C. freundii dan P. pneumotropica tidak virulen pada ikan gabus, semah, dan baung. Batas dosis atas untuk antibiotik berdasarkan petunjuk penggunaan obat untuk enrofloksasin adalah 10 mg/L; eritromisin 3,75 mg/L; dan oksitetrasiklin 250 mg/L. Hasil uji kerentanan dengan metode difusi zona hambat menunjukkan bahwa telah terjadi resistensi pada bakteri Pantoea spp., E. faecium, dan A. hydrophila terhadap antibiotik enrofloksasin dan eritromisin, dan resistensi pada bakteri E. faecium terhadap oksitetrasiklin.Identification of microflora in freshwater fish is an important tool to evaluate the health quality of fish cultured in intensive aquaculture systems. This study aimed to investigate bacteria in freshwater fish, determine their virulence and test their susceptibility to antibiotics. The freshwater fish species of interest in this study were Channa striata spp., and Hemibagrus sp. originated from wild catch and fish farming ponds. Isolated bacteria from kidney, liver, skin wound, and brain tissue were identified using physical and biochemical tests followed by API 20 E and API 20 Strep KIT tests. Pathogenicity test of each bacterial isolate was carried out by intramuscular injection of 106 CFU mL-1 of bacterial suspension per fish. Snakehead fish (Channa striata) was susceptible to Pasteurella pneumotropica and Aeromonas hydrophila infections, Cyprinidae (Tor spp.) was susceptible to Enterococcus faecium, Pantoea spp., and A. hydrophila, while Catfish (Hemibagrus sp.) was susceptible to Citrobacter freundii and A. hydrophila infections. Bacteria E. faecium, Pantoea sp. and A. hydrophila have a low virulence level of 3.3%-23.4%, whereas C. freundii and P. pneumotropica were both non-virulent to snakehead, cyprinidae, and catfish. The highest recommended dosages for enrofloxacin, erythromycin, and oxytetracycline were 10 mg/L, 3.75 mg/L, and 250 mg/L, respectively. The results of antibiotics sensitivity test showed that Pantoea spp., E. faecium, and A. hydrophila bacteria were resistant to enrofloxacin and erythromycin, while E. faecium bacteria was resistant to oxytetracycline.
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Bulmakova, D. S., G. I. Shagieva, D. L. Itkinaa, O. A. Leninа, M. R. Sharipova, and A. D. Suleimanova. "Antagonistic Strains of <i>Pantoea brenneri</i> as Plant Protectors." Микология и фитопатология 57, no. 5 (2023): 352–61. http://dx.doi.org/10.31857/s0026364823050033.
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The antagonistic activity of Pantoea brenneri strains against a wide range of phytopathogenic threats was studied. It has been established that the strains are characterized by fungicidal activity against the micromycetes Fusarium sambucinum, F. oxysporum, F. solani, Rhizoctonia solani, Alternaria sp., Ascochyta kamchatica, Colletotrichum coccodes as well as antibacterial activity against the phytopathogen Erwinia amylovora, which causes bacterial burn of fruit trees. It has been shown that the cell suspension and supernatant of the culture liquid of Pantoea brenneri strains suppress Fusarium on potato tubers during storage. Pantoea brenneri strains have been found to be safe for model animals. A conclusion was made about the prospects of using P. brenneri strains as objects for the creation of environmentally friendly plant protection products against phytopathogens.
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Thanh, Bùi Thị Hoài, Nguyễn Ngọc Thiên Hương, Lê Quốc Việt, Trần Văn Bé Năm, and Trần Thị Giang. "EVALUATION OF THE ABILITY OF IAA BIOSYNTHESIS AND THE EFFECT TO GERMINATION ON MAIZE KERNELS OF HALOTOLERANT PHOSPHATE-SOLUBILIZING BACTERIA Pantoea sp. TTB4.1." TNU Journal of Science and Technology 230, no. 01 (2024): 91–97. https://doi.org/10.34238/tnu-jst.10727.
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Vi khuẩn thúc đẩy sinh trưởng thực vật (PGPR) là nhóm vi khuẩn có lợi cho cây trồng trong đó nhóm vi khuẩn hòa tan lân (PSB) được quan tâm bởi khả năng hỗ trợ cây trồng chống chịu với stress mặn và sản sinh chất kích thích sinh trưởng thực vật. Nghiên cứu này nhằm khảo sát khả năng tổng hợp chất kích thích sinh trưởng thực vật indole acetic acid (IAA) của chủng vi khuẩn hòa tan lân chịu mặn Pantoea sp. TTB4.1 và khảo sát ảnh hưởng đến khả năng nảy mầm của hạt ngô khi ngâm trong dịch huyền phù vi khuẩn. Từ kết quả nghiên cứu, trong 10 ngày nuôi cấy chủng vi khuẩn Pantoea sp. TTB4.1 có khả năng tổng hợp IAA với hàm lượng từ 1,163 - 3,916 µg/mL, trong đó hàm lượng IAA đạt cao nhất vào ngày 4. Đồng thời, nghiên cứu này cũng đánh giá tác động của chủng vi khuẩn hòa tan lân chịu mặn Pantoea sp. TTB4.1 đến khả năng nảy mầm của hạt ngô bằng các nghiệm thức ngâm trong dịch huyền phù vi khuẩn trong 30, 60, 90 phút và các kết quả chiều dài rễ, chiều dài mầm, số rễ và tỷ lệ nảy mầm của hạt ngô ghi nhận đều cao hơn khi so sánh với nghiệm thức đối chứng (không ngâm vi khuẩn).
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Morales Ramos, Jorge Guillermo, Leydy Mekinley Fernández Tarrillo, Anghelly Xiomara Guevara Bravo, et al. "Efficiency of Microorganisms and Effectiveness of Biodegradation Techniques on LDPE Plastics: A Systematic Review." F1000Research 13 (September 2, 2024): 745. http://dx.doi.org/10.12688/f1000research.151338.2.
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Introduction The aim of the research was to demonstrate the efficiency of microorganisms and the effectiveness of biodegradation techniques on Low-density polyethylene (LDPE) plastics. The research question was: What is the efficiency of LDPE-degrading microorganisms and the effectiveness of biodegradation techniques? Methods The systematic review was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Articles were obtained from Scopus, Web of Science (WOS), Embase, and Google Scholar. The DeCS/Mesh search terms were: Low-density polyethylene, efficiency, biodegradation, microbial consortia, fungi, bacteria. Inclusion criteria were: scientific articles that included bacteria, fungi, and microbial consortia reported as LDPE degraders that report the percentage of weight loss; articles published from January 2010 to October 2022, and publications in Spanish and English with open access. Exclusion criteria were: studies that do not report gravimetry, the biodegradation time of LDPE, and the genus or species of the polyethylene-degrading microorganism. Results Out of 483 studies found, 50 were included in this Systematic Review (SR). The most frequent study techniques were scanning electron microscopy (SEM), gravimetry, and fourier transform infrared spectroscopy (FTIR), and in the case of microorganisms, the most studied belonged to the genus Pseudomonas, Bacillus, and Aspergillus. Regarding the isolation place, the most frequent mentioned in the reviewed articles were landfill soil and sanitary landfill soil. The efficiency of LDPE-degrading microorganisms was higher in bacteria such as Enterobacter spp., Pantoea spp., Pseudomonas spp., Escherichia coli, and Bacillus spp., which obtained a range of DE of 9.00-70.00%, 24.00-64%, 1.15 – 61.00%, 45.00%, and 1.5-40% with DT of 4-150, 120, 4-150, 30, and 30-120 days, respectively; in the case of fungi, the main microorganisms are Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii with efficiencies of 54.34, 48.78, and 46.34%, in 90 days, respectively; and the most efficient microbial consortia were from Enterobacter spp. and Pantoea sp. with 38.00 – 81.00%, in 120 days; and, Pseudomonas protegens, Stenotrophomonas sp., B. vallismortis and Paenibacillus sp. with 55. 00 – 75.00% in 120 days. Conclusions The most efficient microorganisms in LDPE degradation are Enterobacter spp., Pantoea spp., Pseudomonas spp., Escherichia coli, and Bacillus spp.; in fungi Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii; and in microbial consortia, those formed by Enterobacter spp. and Pantoea sp., and that of P. protegens, Stenotrophomonas sp., B. vallismortis and Paenibacillus sp.; and the most effective techniques used in LDPE biodegradation are SEM, gravimetry, and FTIR.
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Morales Ramos, Jorge Guillermo, Leydy Mekinley Fernández Tarrillo, Anghelly Xiomara Guevara Bravo, et al. "Efficiency of Microorganisms and Effectiveness of Biodegradation Techniques on LDPE Plastics: A Systematic Review." F1000Research 13 (July 4, 2024): 745. http://dx.doi.org/10.12688/f1000research.151338.1.
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Introduction The aim of the research was to demonstrate the efficiency of microorganisms and the effectiveness of biodegradation techniques on Low-density polyethylene (LDPE) plastics. The research question was: What is the efficiency of LDPE-degrading microorganisms and the effectiveness of biodegradation techniques? Methods The systematic review was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Articles were obtained from Scopus, Web of Science (WOS), Embase, and Google Scholar. The DeCS/Mesh search terms were: Low-density polyethylene, efficiency, biodegradation, microbial consortia, fungi, bacteria. Inclusion criteria were: scientific articles that included bacteria, fungi, and microbial consortia reported as LDPE degraders that report the percentage of weight loss; articles published from January 2010 to October 2022, and publications in Spanish and English with open access. Exclusion criteria were: studies that do not report gravimetry, the biodegradation time of LDPE, and the genus or species of the polyethylene-degrading microorganism. Results Out of 483 studies found, 50 were included in this Systematic Review (SR). The most frequent study techniques were scanning electron microscopy (SEM), gravimetry, and fourier transform infrared spectroscopy (FTIR), and in the case of microorganisms, the most studied belonged to the genus Pseudomonas, Bacillus, and Aspergillus. Regarding the isolation place, the most frequent mentioned in the reviewed articles were landfill soil and sanitary landfill soil. The efficiency of LDPE-degrading microorganisms was higher in bacteria such as Enterobacter spp., Pantoea spp., Pseudomonas spp., Escherichia coli, and Bacillus spp., which obtained a range of DE of 9.00-70.00%, 24.00-64%, 1.15 – 61.00%, 45.00%, and 1.5-40% with DT of 4-150, 120, 4-150, 30, and 30-120 days, respectively; in the case of fungi, the main microorganisms are Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii with efficiencies of 54.34, 48.78, and 46.34%, in 90 days, respectively; and the most efficient microbial consortia were from Enterobacter spp. and Pantoea sp. with 38.00 – 81.00%, in 120 days; and, Pseudomonas protegens, Stenotrophomonas sp., B. vallismortis and Paenibacillus sp. with 55. 00 – 75.00% in 120 days. Conclusions The most efficient microorganisms in LDPE degradation are Enterobacter spp., Pantoea spp., Pseudomonas spp., Escherichia coli, and Bacillus spp.; in fungi Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii; and in microbial consortia, those formed by Enterobacter spp. and Pantoea sp., and that of P. protegens, Stenotrophomonas sp., B. vallismortis and Paenibacillus sp.; and the most effective techniques used in LDPE biodegradation are SEM, gravimetry, and FTIR.
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Paliwoda, Dominika, Grzegorz Mikiciuk, Małgorzata Mikiciuk, et al. "The Use of Plant Growth Promoting Rhizobacteria to Reduce Greenhouse Gases in Strawberry Cultivation under Different Soil Moisture Conditions." Agronomy 13, no. 3 (2023): 754. http://dx.doi.org/10.3390/agronomy13030754.
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One of the main causes of climate change is the emission of GHGs, and one of the sources for the generation of such gasses is agriculture via plant production. Considering the foregoing, a study was conducted to assess PGPRs in strawberry cultivation which were able to limit GHG emissions. The first experimental factor was the inoculation of plant roots with the Bacillus sp. strains DLGB3, DKB26, DKB58, and DKB 84; the Pantoea sp. strains DKB63, DKB64, DKB65, and DKB68; Azotobacter sp. AJ 1.2; and Pseudomonas sp. PJ 1.1. The second experimental factor constituted the different moisture levels of the growth substrate. In the experiment, emissions of NH3, CO2, N2O, and CH4 were measured. In light of the conducted research, five strains were selected (Azotobacter sp. AJ 1.2; Pantoea sp. DKB64, DKB63, and DKB68; and Pseudomonas sp. strain PJ 1.1) that showed the greatest potential for reducing GHG emissions depending on the prevailing environmental conditions. The application of the tested bacterial strains under different moisture conditions in the substrate either reduced or did not affect GWP. This research on PGPR, which was conducted to select strains of rhizosphere bacteria that would be able to reduce GHG emissions, may form the basis for creating an inoculum and can be employed as an effective strategy for mitigating certain abiotic stresses.
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Bolonhez, Jaqueline Forestieri, Ana Cristina Medeiros Gurgel, Maria Gabriela Lopes, Eduardo Fenili Oliveira, Beatriz Medeiros Gurgel, and Luiz Felipe Blanco. "SEPSE NEONATAL TARDIA POR PANTOEA SP ‐ RELATO DE CASO." Brazilian Journal of Infectious Diseases 25 (January 2021): 101461. http://dx.doi.org/10.1016/j.bjid.2020.101461.
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Bình, Trần An, Trương Thị Anh Thư, Nguyễn Vĩnh Thái та ін. "TỐI ƯU HÓA ĐIỀU KIỆN NUÔI CẤY DÒNG VI KHUẨN CHỊU MẶN CÓ KHẢ NĂNG HÒA TAN LÂN Pantoea sp. TTB4.1". Tạp chí Khoa học và Công nghệ Lâm nghiệp, № 1 (2023): 3–11. http://dx.doi.org/10.55250/jo.vnuf.2023.1.003-011.
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Mục tiêu của nghiên cứu này là chọn lọc được nguồn carbon và nitrogen trong môi trường nuôi cấy dòng vi khuẩn chịu mặn có khả năng hòa tan lân Pantoea sp. TTB4.1 cho hàm lượng lân hòa tan cao nhất. Đồng thời nghiên cứu này cũng thực hiện tối ưu hóa các yếu tố ảnh hưởng đến khả năng hòa tan lân của dòng vi khuẩn Pantoea sp. TTB4.1 theo mô hình RSM-CCD (Response surface methodology-Central composite design). Bốn yếu tố được tối ưu bao gồm nồng độ carbon (5-15 g/L), nồng độ nitrogen (0,05-0,15 g/L), pH ban đầu (6,0-8,0), và mật số giống chủng (7,7-11,7 log(CFU/mL)). Kết quả cho thấy rằng glucose và (NH4)2SO4 lần lượt là nguồn carbon và nitrogen thích hợp để dòng vi khuẩn Pantoea sp. TTB4.1 hòa tan lân cao nhất. Bên cạnh đó, kết quả tối ưu hóa theo mô hình RSM-CCD xác định nồng độ glucose từ 11,52-12,49 g/L, nồng độ (NH4)2SO4 từ 0,112-0,118 g/L, pH ban đầu từ 7,4-7,5 và mật số giống chủng từ 10,4-10,7 log(CFU/mL) là điều kiện tối ưu để thu được lượng lân hòa tan tốt nhất từ 1.369,99-1.372,62 mg/L, đạt hiệu suất khoảng 44,8% so với lượng Ca3(PO4)2 (5 g/L) được bổ sung ban đầu.
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Polisetti, Sneha, Amber N. Bible, Jennifer L. Morrell-Falvey, and Paul W. Bohn. "Raman chemical imaging of the rhizosphere bacterium Pantoea sp. YR343 and its co-culture with Arabidopsis thaliana." Analyst 141, no. 7 (2016): 2175–82. http://dx.doi.org/10.1039/c6an00080k.
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Raman micro-spectroscopy and confocal Raman imaging are used to study the rhizosphere bacterial isolate, Pantoea sp. YR343, and its co-culture with model plant Arabidopsis thaliana by combining enhanced Raman spectroscopies with electron microscopy and principal component analysis.
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de Siqueira, Kátia Aparecida, Rhavena Graziela Liotti, Tiago Antônio de Oliveira Mendes, and Marcos Antônio Soares. "Draft Genome Sequences of Pseudomonas sp. Strain 382 and Pantoea coffeiphila 342, Endophytic Bacteria Isolated from Brazilian Guarana [Paullinia cupana (Mart.) Ducke]." Genome Announcements 6, no. 16 (2018): e00287-18. http://dx.doi.org/10.1128/genomea.00287-18.
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ABSTRACT Pseudomonas sp. strain 382 and Pantoea coffeiphila 342 are two endophytic bacterial strains isolated from Paullinia cupana (guarana) seeds. Their draft genome sizes were 5.96 and 6.38 Mbp, with 315 and 266 scaffolds and 52% and 62% GC content, respectively.
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Touré, Seindé, Sandy Desrat, Léonie Pellissier, et al. "Characterization, Diversity, and Structure-Activity Relationship Study of Lipoamino Acids from Pantoea sp. and Synthetic Analogues." International Journal of Molecular Sciences 20, no. 5 (2019): 1083. http://dx.doi.org/10.3390/ijms20051083.
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A biological evaluation of a library of extracts from entomopathogen strains showed that Pantoea sp. extract has significant antimicrobial and insecticidal activities. Three hydroxyacyl-phenylalanine derivatives were isolated from this strain. Their structures were elucidated by a comprehensive analysis of their NMR and MS spectroscopic data. The antimicrobial and insecticidal potencies of these compounds were evaluated, and compound 3 showed 67% mortality against Aedes aegypti larvae at a concentration of 100 ppm, and a minimum inhibitory concentration (MIC) of 16 µg/mL against methicillin-resistant Staphylococcus aureus. Subsequently, hydroxyacyl-phenylalanine analogues were synthesized to better understand the structure-activity relationships within this class of compounds. Bioassays highlighted the antimicrobial potential of analogues containing saturated medium-chain fatty acids (12 or 14 carbons), whereas an unsaturated long-chain fatty acid (16 carbons) imparted larvicidal activity. Finally, using a molecular networking-based approach, several close analogues of the isolated and newly synthesized lipoamino acids were discovered in the Pantoea sp. extract.
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Araújo, Welington L., Walter Maccheroni Jr., Carlos I. Aguilar-Vildoso, Paulo AV Barroso, Halha O. Saridakis, and João Lúcio Azevedo. "Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks." Canadian Journal of Microbiology 47, no. 3 (2001): 229–36. http://dx.doi.org/10.1139/w00-146.
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Fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. The principal bacterial species isolated were Alcaligenes sp., Bacillus spp. (including B. cereus, B. lentus, B. megaterium, B. pumilus, and B. subtilis), Burkholderia cepacia, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium extorquens, and Pantoea agglomerans, with P. agglomerans and B. pumilus being the most frequently isolated species. The most abundant fungal species were Colletotrichum gloeosporioides, Guignardia citricarpa, and Cladosporium sp. Genetic variability between 36 endophytic bacterial isolates was analysed by the random amplified polymorphic DNA (RAPD) technique, which indicated that B. pumilus isolates were more diverse than P. agglomerans isolates, although genetic diversity was not related to the host plants. In vitro interaction studies between G. citricarpa isolates and the most frequently isolated endophytic bacteria showed that metabolites secreted by G. citricarpa have an inhibitory growth effect on some Bacillus species, and a stimulatory growth effect on P. agglomerans.Key words: endophytes, citrus, fungal-bacterial interaction, RAPD, diversity, Pantoea agglomerans, Bacillus pumilus, Guignardia citricarpa.
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Brady, Carrie L., Stephanus N. Venter, Ilse Cleenwerck, Katrien Vandemeulebroecke, Paul De Vos, and Teresa A. Coutinho. "Transfer of Pantoea citrea, Pantoea punctata and Pantoea terrea to the genus Tatumella emend. as Tatumella citrea comb. nov., Tatumella punctata comb. nov. and Tatumella terrea comb. nov. and description of Tatumella morbirosei sp. nov." International Journal of Systematic and Evolutionary Microbiology 60, no. 3 (2010): 484–94. http://dx.doi.org/10.1099/ijs.0.012070-0.
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Pantoea citrea, Pantoea punctata and Pantoea terrea were described for strains isolated from fruit and soil originating in Japan. These three ‘Japanese’ species have been shown to be phylogenetically distant from other species of the genus Pantoea. It has been observed previously that, using multilocus sequence analysis (MLSA), the ‘Japanese’ species consistently formed a distinct clade with an extended branch length, casting doubt on the inclusion of these species within the genus Pantoea. Furthermore, the ‘Japanese’ species are closely related to Tatumella ptyseos, strains of which originate from human clinical specimens. DNA–DNA hybridization and phenotypic tests confirmed the observed phylogenetic distance of P. citrea, P. punctata and P. terrea from the genus Pantoea and the affiliation of these species with Tatumella. In addition, strains causing pink disease of pineapple, identified previously as P. citrea, were shown to represent a separate species by using 16S rRNA gene sequence analysis, and MLSA and DNA–DNA hybridization data. The name Tatumella morbirosei sp. nov. with the type strain LMG 23360T (=BD 878T=NCPPB 4036T=CMC6T) is proposed to accommodate these strains. The new combinations Tatumella citrea (Kageyama et al. 1992) comb. nov. (type strain, SHS 2003T=ATCC 31623T=BD 875T=CCUG 30156T=CIP 105599T=DSM 13699T=JCM 8882T=LMG 22049T), Tatumella punctata (Kageyama et al. 1992) comb. nov. (type strain, SHS 2006T=ATCC 31626T=BD 876T=CCUG 30159T=CIP 105598T=DSM 13700T=JCM 8885T=LMG 22050T) and Tatumella terrea (Kageyama et al. 1992) comb. nov. (type strain, SHS 2008T=ATCC 31628T=BD 877T=CCUG 30161T=CIP 105600T=DSM 13701T=JCM 8887T=LMG 22051T) are proposed for P. citrea, P. punctata and P. terrea, respectively.
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Elmore, Matthew T., James F. White, Kathryn L. Kingsley, Katherine H. Diehl, and Satish K. Verma. "Pantoea spp. Associated with Smooth Crabgrass (Digitaria ischaemum) Seed Inhibit Competitor Plant Species." Microorganisms 7, no. 5 (2019): 143. http://dx.doi.org/10.3390/microorganisms7050143.
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Digitaria ischaemum (Schreb.) Schreb. ex Muhl. and Poa annua L. are competitive, early successional species which are usually considered weeds in agricultural and turfgrass systems. Bacteria and fungi associated with D. ischaemum and P. annua seed may contribute to their competitiveness by antagonizing competitor forbs, and were studied in axenic culture. Pantoea spp. were the most common bacterial isolate of D. ischaemum seed, while Epicoccum and Curvularia spp. were common fungal isolates. A variety of species were collected from non-surface sterilized P. annua. Certain Pantoea spp. isolates were antagonistic to competitor forbs Taraxacum officinale, Trifolium repens. All bacterial isolates that affected T. officinale mortality were isolated from D. ischaemum seed while none of the P. annua isolates affected mortality. Two selected bacterial isolates identified as Pantoea ananatis were evaluated further on D. ischaemum, T. repens (a competitor forb) and P. annua (a competitor grass) alone and in combination with a Curvularia sp. fungus. These bacteria alone caused >65% T. repens seedling mortality but did not affect P. annua seedling mortality. These experiments demonstrate that Pantoea ananatis associated with D. ischaemum seeds is antagonistic to competitor forbs in axenic culture. The weedy character of D. ischaemum could at least in part stem from the possession of bacteria that are antagonistic to competitor species.
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Ong, Zhi-Zhang, Yeak-Heng Chong, and Wey-Lim Wong. "Transient or Permanent Inhabitants: Cultured Internal Microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp." Sains Malaysiana 51, no. 8 (2021): 2339–49. http://dx.doi.org/10.17576/jsm-2022-5108-01.
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Monogeneans, a class of parasitic platyhelminthes, are usually found on the surface of fish. When it feeds on its host, it harbours bacteria, which can survive in its gut. Occasionally, the monogeneans may cause damages on fish tissue while feeding and may allow secondary infection. The present study aimed to isolate and identify culturable bacteria obtained within the gill monogeneans, Cichlidogyrus thurstonaeand Scutogyrus longicornis, and gill surface of the tilapia fish, Oreochromis sp. based on 16S ribosomal RNA gene sequencing. Monogeneans were isolated from the fish gill filaments and surface disinfected using 70% ethanol before squashed aseptically on Luria Bertani (LB) agar to isolate the internal microbiota. A total of five bacteria species, namely Burkholderia sp., Enterobacter hormaechei, Enterobacter sp., Ochrobactrum intermedium and Pantoea sp., were found within C. thurstonae, whilst a total of eight bacteria species, namely Burkholderia contaminans, Pantoea dispersa, Sphingomonas yabuuchiae, Rhizobium pusense, O. intermedium, Acinetobacter bereziniae, Escherichia hermannii, and Staphylococcus saprophyticus, were found within S. longicornis in which B. contaminans, P. dispersa, and S. yabuuchiaewere also found on the surface of the tilapia fish gill filaments. Enterobacter bugandensisand Acinetobacter pittiiwere found solely on the surface of the gill filaments. These bacteria are also found in the environment and some of them are believed to be pathogenic to fish. We suggest that monogeneans may serve as potential bacteria reservoirs, which facilitate the transmission of bacteria.
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Sherline, Sherline, Maharani Dian Permanasari, Dadang Sumardi, Sony Suhandono, and Fenny Martha Dwivany. "16S rRNA-based Metagenomic Analysis of Beeswax-coated Saba Banana (Musa × paradisiaca) Pseudostem." Pertanika Journal of Tropical Agricultural Science 47, no. 3 (2024): 969–83. http://dx.doi.org/10.47836/pjtas.47.3.23.
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Bananas are one of the most popular fruits, and their production generates significant agricultural waste. Banana pseudostems, a by-product of the banana industry, are being investigated as a renewable and biodegradable alternative to synthetic food packaging materials. However, these pseudostems have the potential to harbor harmful bacteria due to their natural fiber composition. Therefore, this study analyzes the effect of beeswax coating on the microbial communities in banana pseudostems. The microbial community is analyzed through a metagenomics approach that targets the 16S rRNA gene of the Saba banana (Musa × paradisiaca) pseudostem. Two experimental conditions were considered: pseudostem with beeswax coating and pseudostem without beeswax coating. The findings indicate that the microbial communities in all samples are primarily composed of the phyla Proteobacteria, Cyanobacteria, and Firmicutes. The dominant species found in uncoated banana pseudostem is Pantoea sp. At-9b, Escherichia coli, Synechococcus sp. JA-3-3-Ab, Pantoea vagans, and Klebsiella pneumoniae. The dominant species found in beeswax-coated banana pseudostem is Synechococcus sp. JA-3-3-Ab, Pseudanabaena sp. PCC 7367, Chroococcidiopsis thermalis, Priestia megaterium, and Ammonifex degensii. The Chao1, Shannon, Simpson, and Equitability indices indicate that the species richness, diversity, and evenness in the uncoated banana pseudostem are higher than in the beeswax-coated banana pseudostem. The degree of similarity between bacterial populations found in uncoated banana pseudostem and beeswax-coated banana pseudostem is around 53.9%.
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Chahed, Abdessalem, Andrea Nesler, Qassim Esmaeel, Essaid Ait Barka, and Michele Perazzolli. "The Amount of the Rare Sugar Tagatose on Tomato Leaves Decreases after Spray Application under Greenhouse Conditions." Plants 11, no. 20 (2022): 2781. http://dx.doi.org/10.3390/plants11202781.
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Tagatose is a rare sugar that suppresses plant diseases, such as late blight of tomato, caused by Phytophthora infestans. Tagatose can be metabolized by some microorganisms and no information is available on its persistence on tomato leaves. The aim of this study was to assess the persistence of tagatose on tomato leaves under commercial greenhouse conditions. The amount of tagatose on tomato leaves and the inhibitory activity against P. infestans decreased seven days after spray application in the absence of rain wash-off. Potential tagatose-degrading bacteria were isolated from tomato leaves, and they belonged to Acinetobacter sp., Bacillus sp., Comamonas sp., Enterobacter sp., Methylobacterium sp., Microbacterium sp., Pantoea sp., Plantibacter sp., Pseudomonas sp., Ralstonia sp., Rhodococcus sp., Sphingobium sp., and Sphingomonas sp. Thus, indigenous phyllosphere microorganisms could partially metabolize tagatose laid on plant leaves after spray application, reducing the persistence of this fungal inhibitor on tomato leaves.
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Lara Mantilla, Cecilia, and Jorge Luis Negrete Peñata. "Efecto de un bioinoculante a partir de consorcios microbianos nativos fosfato solubilizadores, en el desarrollo de pastos Angleton (Dichantium aristatum) Titulo en ingles: Effect of bio-inoculant from microbial consortia phosphate solu." Revista Colombiana de Biotecnología 17, no. 1 (2015): 122–30. http://dx.doi.org/10.15446/rev.colomb.biote.v17n1.50741.
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<strong>Título en ingles: Effect of bio-inoculant from microbial consortia phosphate solubilizing natives in development of pastures <em>(Dichantium aristatum)</em></strong><em></em><p><em></em><strong>Título corto:</strong> <strong>Efecto de un bioinoculante en pastos Angleton</strong></p><p><strong>Resumen</strong>: El objetivo fue evaluar la capacidad solubilizadora de fosfatos de consorcios formados por bacterias nativas de los géneros <em>Burkholderia cepacia, Pseudomonas </em>sp<em>, Pseudomonas luteola y Pantoea </em>sp, con el fin de encontrar el más eficiente. Se realizaron pruebas de antagonismo entre las cepas y se formaron consorcios probando todas las combinaciones posibles en las concentraciones de 10<sup>6</sup>, 10<sup>7</sup>, 10<sup>8 </sup>UFC/mL. Se realizaron evaluaciones cualitativas y cuantitativas de la solubilización de fosfatos y teniendo en cuenta éstos resultados, se preparó un bioinoculante el cual fue evaluado en semillas de plantas de pasto angleton (<em>Dichantium aristatum) </em>a escala de laboratorio, utilizando un diseño estadístico completamente al azar (DCA) con 3 tratamientos y 5 repeticiones: Tratamiento 1 semillas (control), Tratamiento 2, semillas tratadas con el consorcio de microorganismos seleccionado y Tratamiento 3, semillas tratadas con fertilizantes comerciales DAP y Urea. Se evaluaron las variables número de hojas, área foliar, longitud de la planta, longitud de la raíz y peso seco de todas las plantas. Los resultados de la prueba de antagonismo indicaron que no existe inhibición en el crecimiento de las cepas evaluadas, por lo tanto se formaron consorcios los cuales mostraron mayor eficiencia en la solubilización del fósforo, destacándose el consorcio formado por <em>Pantoea </em>sp<em> + Pseudomonas </em>sp<em> </em>a una concentración de 10<sup>8</sup> UFC/mL y<em> </em>con índices de solubilización de 5,3 y 842 ppm<em>.</em> En las plantas se evidenció un incremento significativo en los parámetros peso seco y área foliar usando el consorcio microbiano, indicando mayor beneficio en comparación con el control.<strong></strong></p><p><strong>Palabras clave: </strong><em>Burkholderia cepacia, Pseudomonas </em>sp,<em> Pseudomonas luteola, Pantoea </em>sp<em>, </em>bioinoculante. <em></em><strong></strong></p><p><strong>Abstract</strong>: The objective was to evaluate the ability of phosphate solubilizing consortium of native bacteria of the <em>genus</em> <em>Burkholderia cepacia</em>, <em>Pseudomonas </em>sp, <em>Pseudomonas luteola</em> and <em>Pantoea </em>sp, in order to find the most efficient. Antagonism tests were conducted between strains and consortia were formed using all possible combinations in the concentrations of 10<sup>6</sup>, 10<sup>7</sup>, 10<sup>8</sup> CFU / mL. Qualitative and quantitative determinations of the solubilization of phosphates were performed and considering these results, was prepared a bio-inoculant which was evaluated in plant seeds of grass angleton (Dichantium aristatum) laboratory scale, using a statistical completely randomized design (CRD) with 3 treatments and 5 repetitions: control Treatment 1 seeds; Treatment 2, seeds treated with the consortium of microorganisms selected and Treatment 3, seeds treated with commercial fertilizers DAP and Urea. The parameters, number of leaves, leaf area, plant length, root length and dry weights of all plants, were evaluated. The test results indicated that there is no antagonism inhibition in the growth of the strains tested thus formed consortia which showed greater efficiency phosphorus solubilization, highlighting the consortium of Pantoea sp + Pseudomonas sp at a concentration 10<sup>8</sup> CFU / mL and 5.3 solubilization rates and 842 ppm. In plants showed a significant increase in dry weight and leaf area parameters, indicating greater benefit with respect to the control treatment.</p><p><strong>Key words:</strong> <em>Burkholderia cepacia</em>, <em>Pseudomonas</em> sp, <em>Pseudomonas luteola</em>, <em>Pantoea</em> sp, bio-inoculant.<strong></strong></p><p><strong>Recibido: </strong>marzo 15 de 2014 <strong> Aprobado: </strong>abril 15 de 2015</p>
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RAJENDRA, PERSAUD, GILKES JOMEALA, CASEY AKEIM, DARSHANIE ANGELA PERSAUD, and CHARLES ELROY. "DISCOVERY OF CURVULARIA SP., PANTOEA SP., AND PSEUDOMONAS ORYZIHABITANS, ASSOCIATED WITH SYMPTOMS OF DIRTY DISCOLORED GRAINS OF PADDY AND PANICLE BLIGHT IN GUYANA." Greener Journal of Agricultural Sciences 14, no. 1 (2024): 15–22. https://doi.org/10.15580/gjas.2024.1.012324014.
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Rice (Oryza sativa L.) dirty grains and blighted panicles symptoms were observed on about 1-5% of plants within farmers’ fields in Essequibo (region#2,3), Demerara (reg.#4,5) and Berbice (reg.#6) in Guyana. Four samples at maturity stage with Dirty Discolored Grains of Paddy and Panicle Blight were harvested, labeled and place in paper bags in March 2022 from regions#2, 3, 4 and 5 for diagnosis and molecular confirmation. Initial identification to genus-level was done using morphological features and MALDI-ToF. Results of the morphological and molecular sequencing identifies fungal isolates Curvularia sp., with top matches of >99% for C. lunata, C. dactyloctenicola, and >98% for C. inaequalis. Similarly, sequencing analysis identified Pantoea sp. with matches of >99% to several closely related species [including P. stewartia P. allii, P. ananatis, P. agglomerans and P. pleuroti] and Pseudomonas oryzihabitans with top matches of >99% (European Molecular Biology Laboratory (EMBL) database via the European Bioinformatics Institute (EBI), (CABI, 2023). Pathogenicity tests were carried out following Koch’s postulate and confirmed that Curvularia sp., Pantoea sp., and Pseudomonas oryzihabitans were the pathogens responsible for dirty discolored grains of paddy and panicle blight symptoms observed, while the control plants remained healthy and symptomless. To our knowledge, this is the first report that identifies and confirm these 3 pathogens working together and causing the dirty discolored grains of paddy and blighted panicle symptoms in Guyana.
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GAVINI, F., J. MERGAERT, A. BEJI, et al. "Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and Description of Pantoea dispersa sp. nov." International Journal of Systematic Bacteriology 39, no. 3 (1989): 337–45. http://dx.doi.org/10.1099/00207713-39-3-337.
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Wiriya, Janjira, Chakrapong Rangjaroen, Neung Teaumroong, Rungroch Sungthong, and Saisamorn Lumyong. "Rhizobacteria and Arbuscular Mycorrhizal Fungi of Oil Crops (Physic Nut and Sacha Inchi): A Cultivable-Based Assessment for Abundance, Diversity, and Plant Growth-Promoting Potentials." Plants 9, no. 12 (2020): 1773. http://dx.doi.org/10.3390/plants9121773.
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Nowadays, oil crops are very attractive both for human consumption and biodiesel production; however, little is known about their commensal rhizosphere microbes. In this study, rhizosphere samples were collected from physic nut and sacha inchi plants grown in several areas of Thailand. Rhizobacteria, cultivable in nitrogen-free media, and arbuscular mycorrhizal (AM) fungi were isolated and examined for abundance, diversity, and plant growth-promoting activities (indole-3-acetic acid (IAA) and siderophore production, nitrogen fixation, and phosphate solubilization). Results showed that only the AM spore amount was affected by plant species and soil features. Considering rhizobacterial diversity, two classes—Alphaproteobacteria (Ensifer sp. and Agrobacterium sp.) and Gammaproteobacteria (Raoultella sp. and Pseudomonas spp.)—were identified in physic nut rhizosphere, and three classes; Actinobacteria (Microbacterium sp.), Betaproteobacteria (Burkholderia sp.) and Gammaproteobacteria (Pantoea sp.) were identified in the sacha inchi rhizosphere. Considering AM fungal diversity, four genera were identified (Acaulospora, Claroideoglomus, Glomus, and Funneliformis) in sacha inchi rhizospheres and two genera (Acaulospora and Glomus) in physic nut rhizospheres. The rhizobacteria with the highest IAA production and AM spores with the highest root-colonizing ability were identified, and the best ones (Ensifer sp. CM1-RB003 and Acaulospora sp. CM2-AMA3 for physic nut, and Pantoea sp. CR1-RB056 and Funneliformis sp. CR2-AMF1 for sacha inchi) were evaluated in pot experiments alone and in a consortium in comparison with a non-inoculated control. The microbial treatments increased the length and the diameter of stems and the chlorophyll content in both the crops. CM1-RB003 and CR1-RB056 also increased the number of leaves in sacha inchi. Interestingly, in physic nut, the consortium increased AM fungal root colonization and the numbers of offspring AM spores in comparison with those observed in sacha inchi. Our findings proved that AM fungal abundance and diversity likely rely on plant species and soil features. In addition, pot experiments showed that rhizosphere microorganisms were the key players in the development and growth of physic nut and sacha inchi.
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ELISYUTIKOVA, A. V., A. A. TABACHNIKOVA, and I. L. ASTAPCHUK. "STUDYING BACTERIAL COMMUNITIES OF THE GRAPES PHYLOSPHERE." Scientific Works of North Caucasian Federal Scientific Center of Horticulture Viticulture Wine-making, no. 38 (December 2024): 46–49. https://doi.org/10.30679/2587-9847-2024-38-46-49.
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As a result, of the conducted research, 20 bacterial strains were isolated from the phyllosphere of grapes selected by the Kuban State Agrarian University, 19 of which were identified by MALDI-TOF MS mass spectrometry as Bacillus sp., 1 as Pantoea agglomerans . As a result, of evaluating their antagonism towards phytopathogens during in vitro joint cultivation, 3 - strains were isolated from disease-resistant grape samples, 2 strains from unstable ones. Each variety had its own specific complex of microscopic bacteria. Keywords: MICROBIOME, PHYLLOSPHERE, GRAPEVINE, BACILLUS SP, MALDI-TOF MS, ANTAGONISM
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Suleimanova, Aliya D., Daria S. Bulmakova, Daria L. Itkina, and Margarita R. Sharipova. "Expression of Pantoea sp. 3.5.1 AgpP Phytase in Three Expression Systems." BioNanoScience 11, no. 2 (2021): 648–52. http://dx.doi.org/10.1007/s12668-021-00855-0.
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Mohmmed Lefta Atala. "The UV light effects on pectinase production of Pantoea sp. bacteria." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (2020): 4578–82. http://dx.doi.org/10.26452/ijrps.v11i3.2689.
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The highest pectinase producing isolate A3 out of forty isolates of Pantoea sp. bacteria was subjected to physical effects by using UV light to change in their ability for producing of pectinase, the results appeared increasing their activity and production in some of the mutants more than wild type bacteria, the increase was ranged between 14 to 20% in specific activity between bacterial mutants compared with wild type bacteria. Also, the two highest enzyme-producing isolates were tested for their susceptibility against a group of antibiotics (Rifampin 50mcg, Imipenem 10mcg, Vancomycin 30mcg, Amoxicillin 25mcg, Chloramphenicol 30mcg, Trimethoprim 5mcg, Nitrofurantoin 100mcg, Nalidixic acid 30mcg), results appeared different susceptibilities ranged between resist for amoxicillin,and Nalidixic acid, and sensitive for other antibiotics. The susceptibilities of studied bacteria to some antibiotics were different between resist and sensitive, which considered an indicator of dangerous to human life. The study was aimed to increase the ability of these isolate in producing this enzyme which plays an essential role in different industries in food, cosmetics manufacturing and scientific research, besides, their ability in resisting some of the common antibiotics to confirm the transferring of genetic materials between bacteria that isolated from soil samples, which is considered crucial in the microbial genetics. The study appeared there were apparent effects on the genes that responsible for enzyme production either for increase or decrease their activity and creation of an enzyme, which belong to using physical effects to improvement these feature in bacteria.