Academic literature on the topic 'Paramecium'

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Journal articles on the topic "Paramecium"

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Potekhin, Alexey, and Rosaura Mayén-Estrada. "Paramecium Diversity and a New Member of the Paramecium aurelia Species Complex Described from Mexico." Diversity 12, no. 5 (May 15, 2020): 197. http://dx.doi.org/10.3390/d12050197.

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Paramecium (Ciliophora) is an ideal model organism to study the biogeography of protists. However, many regions of the world, such as Central America, are still neglected in understanding Paramecium diversity. We combined morphological and molecular approaches to identify paramecia isolated from more than 130 samples collected from different waterbodies in several states of Mexico. We found representatives of six Paramecium morphospecies, including the rare species Paramecium jenningsi, and Paramecium putrinum, which is the first report of this species in tropical regions. We also retrieved five species of the Paramecium aurelia complex, and describe one new member of the complex, Paramecium quindecaurelia n. sp., which appears to be a sister species of Paramecium biaurelia. We discuss criteria currently applied for differentiating between sibling species in Paramecium. Additionally, we detected diverse bacterial symbionts in some of the collected ciliates.
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Bator, Tomasz. "A New Laboratory Cultivation of Paramecium bursaria Using Non-Pathogenic Bacteria Strains." Zeitschrift für Naturforschung C 65, no. 7-8 (August 1, 2010): 479–82. http://dx.doi.org/10.1515/znc-2010-7-810.

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In most studies dealing with the laboratory cultivation of paramecia (Paramecium bursaria), Klebsiella pneumoniae bacteria are used to inoculate the medium. However, Klebsiella pneumoniae is a typical pathogen, and its use is always associated with a risk of infection. The aim of the present research was to examine non-pathogenic bacteria strains as components of the medium for Paramecium bursaria. The paramecia were incubated on lettuce infusions bacterized with different bacteria strains: Bacillus subtilis DSM 10, Bacillus megaterium DSM 32, Escherichia coli DSM 498, Micrococcus luteus DSM 348. A strain derived from the natural habitat of Paramecium bursaria was used as the control one. Experiments were conducted under constant light and in the dark. Paramecia cells were counted under a stereomicroscope on consecutive days of incubation. The obtained results show that the most intensive growth of Paramecium bursaria occurs in the presence of Escherichia coli DSM 498. The use of this strain as a component of the medium allows one to obtain a high number of ciliates regardless of the light conditions. It can be concluded that the Paramecium bursaria cultivation procedure can be modified by using the non-pathogenic bacteria strain Escherichia coli DSM 498 instead of Klebsiella pneumoniae.
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Kawano, Tomonori, Takashi Kadono, Toshikazu Kosaka, and Hiroshi Hosoya. "Green Paramecia as an Evolutionary Winner of Oxidative Symbiosis: A Hypothesis and Supportive Data." Zeitschrift für Naturforschung C 59, no. 7-8 (August 1, 2004): 538–42. http://dx.doi.org/10.1515/znc-2004-7-816.

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AbstractA single cell of the green paramecia (Paramecium bursaria) harbors several hundreds of endo-symbiotic Chlorella-like algae in its cytoplasm. Removal of algae from the host organism and re-association of ex-symbiotic host paramecia with ex-symbiotic algae can be experimentally demonstrated in the laboratory. However, the mechanism precisely governing the alga-protozoan association is not fully understood, and the origin of symbiosis in the evolutionary view has not been given. Here, we propose the possible biochemical models (models 1 and 2) explaining the co-evolution between Paramecium species and algal symbionts by pointing out that algal photosynthesis in the host paramecia plays a dual role providing the energy source and the risk of oxidative damage to the host. Model 1 lays stress on the correlation between the (re)greening ability of the paramecia and the tolerance to oxidative stress whereas model 2 emphasizes the cause of evolutionary selection leading to the emergence of Paramecium species tolerant against reactive oxygen species.
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Otsuka, Kohei, Sayaka Maruta, Atsuko Noriyasu, Kohji Nakazawa, and Tomonori Kawano. "Single Cell Traffic of Swimming Green Paramecia on Microchips with Micro-Flow Channels Fabricated by Micro-Casting." Advanced Materials Research 875-877 (February 2014): 2224–28. http://dx.doi.org/10.4028/www.scientific.net/amr.875-877.2224.

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Members of Paramecium species are often referred to as “swimming neurons or sensory cells” applicable to micro-biorobotics or BioMEMS (biological micro-electro-mechanical systems). Paramecium bursaria known as green paramecia is an unicellular organism that lives widely in fresh water environments such as rivers and ponds. Recent studies have suggested that in vivo cellular robotics using the living cells of green paramecia as micro-machines controllable under electrical, optical and magnetic signals, has a variety of engineering applications such as transportation of micro-sized particles (ingested within the cells) in the capillary systems. In the present study, we aimed to test if the swimming environment of green paramecia can be implementable on microchips. For this purpose, the series of microchips were prepared for cellular swimming platform for green paramecia through fabrication of poly(methyl methacrylate) master plates using the programmable micro-milling system followed by polydimethylsiloxane-based micro-casting. Finally, microchips equipped with optimally sized micro-flow channels for allowing the single cell traffic by swimming green paramecia were successfully prepared, and thus further studies for application of green paramecium cells in BioMEMS are encouraged.
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Bator, Tomasz, and Ryszard Pado. "The Infl uence of Hypergravity on the Paramecium bursaria- Chlorella sp. Symbiotic Association." Zeitschrift für Naturforschung C 64, no. 9-10 (October 1, 2009): 743–46. http://dx.doi.org/10.1515/znc-2009-9-1021.

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The aim of the research was to determine the influence of strong hypergravity on the Paramecium bursaria-Chlorella sp. symbiotic association, which is considered to be a model example of symbiosis between a heterotroph and an autotroph. The paramecia cells were exposed to 1073 × g, 4293 × g, and 9658 × g hypergravity for 15 min. Then they were incubated for 21 d on a standard lettuce medium. The experiments were conducted in parallel under constant white light and in the dark. The changes in the number of paramecia cells during incubation were determined. Measurements of the number of Chlorella sp. endosymbionts inside host cells were also made. The results showed that a 15-min exposure to hypergravity attenuates the subsequent growth of Paramecium bursaria in the dark, but it may stimulate the growth of paramecia under constant light. Moreover, it causes an increase in the number of algae inside the paramecia cells. Presumably, the influence of hypergravity on the studied symbiotic complex is connected with its effect on the endosymbiotic Chlorella sp. cells. This subject requires further research, focused on the influence of hypergravity on the physiology and growth of the Chlorella sp. endosymbionts living inside the Paramecium bursaria cells
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Elices, Irene, Anirudh Kulkarni, Nicolas Escoubet, Léa-Laetitia Pontani, Alexis Michel Prevost, and Romain Brette. "An electrophysiological and kinematic model of Paramecium, the “swimming neuron”." PLOS Computational Biology 19, no. 2 (February 9, 2023): e1010899. http://dx.doi.org/10.1371/journal.pcbi.1010899.

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Paramecium is a large unicellular organism that swims in fresh water using cilia. When stimulated by various means (mechanically, chemically, optically, thermally), it often swims backward then turns and swims forward again in a new direction: this is called the avoiding reaction. This reaction is triggered by a calcium-based action potential. For this reason, several authors have called Paramecium the “swimming neuron”. Here we present an empirically constrained model of its action potential based on electrophysiology experiments on live immobilized paramecia, together with simultaneous measurement of ciliary beating using particle image velocimetry. Using these measurements and additional behavioral measurements of free swimming, we extend the electrophysiological model by coupling calcium concentration to kinematic parameters, turning it into a swimming model. In this way, we obtain a model of autonomously behaving Paramecium. Finally, we demonstrate how the modeled organism interacts with an environment, can follow gradients and display collective behavior. This work provides a modeling basis for investigating the physiological basis of autonomous behavior of Paramecium in ecological environments.
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Aonuma, Miki, Takashi Kadono, and Tomonori Kawano. "Inhibition of Anodic Galvanotaxis of Green Paramecia by T-Type Calcium Channel Inhibitors." Zeitschrift für Naturforschung C 62, no. 1-2 (February 1, 2007): 93–102. http://dx.doi.org/10.1515/znc-2007-1-217.

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Calcium ion (Ca2+) is one of the key regulatory elements for ciliary movements in the Paramecium species. It has long been known that members of Paramecium species including green paramecia (Paramecium bursaria) exhibit galvanotaxis which is the directed movement of cells toward the anode by swimming induced in response to an applied voltage. However, our knowledge on the mode of Ca2+ action during green paramecia anodic galvanotactic response is still largely limited. In the present study, quantification of anodic galvanotaxis was carried out in the presence and absence of various inhibitors of calcium signaling and calcium channels. Interestingly, galvanotactic movement of the cells was completely inhibited by a variety of Ca2+-related inhibitors. Such inhibitors include a Ca2+ chelator (EGTA), general calcium channel blockers (such as lanthanides), inhibitors of intracellular Ca2+ release (such as ruthenium red and neomycin), and inhibitors of T-type calcium channels (such as NNC 55-0396, 1-octanol and Ni2+). However, L-type calcium channel inhibitors such as nimodipine, nifedipine, verapamil, diltiazem and Cd2+ showed no inhibitory action. This may be the first implication for the involvement of T-type calcium channels in protozoan cellular movements.
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Yakovleva, Yulia, Elena Nassonova, Natalia Lebedeva, Olivia Lanzoni, Giulio Petroni, Alexey Potekhin, and Elena Sabaneyeva. "The first case of microsporidiosis in Paramecium." Parasitology 147, no. 9 (April 27, 2020): 957–71. http://dx.doi.org/10.1017/s0031182020000633.

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AbstractA new microsporidian species, Globosporidium paramecii gen. nov., sp. nov., from Paramecium primaurelia is described on the basis of morphology, fine structure, and SSU rRNA gene sequence. This is the first case of microsporidiosis in Paramecium reported so far. All observed stages of the life cycle are monokaryotic. The parasites develop in the cytoplasm, at least some part of the population in endoplasmic reticulum and its derivates. Meronts divide by binary fission. Sporogonial plasmodium divides by rosette-like budding. Early sporoblasts demonstrate a well-developed exospore forming blister-like structures. Spores with distinctive spherical shape are dimorphic in size (3.7 ± 0.2 and 1.9 ± 0.2 μm). Both types of spores are characterized by a thin endospore, a short isofilar polar tube making one incomplete coil, a bipartite polaroplast, and a large posterior vacuole. Experimental infection was successful for 5 of 10 tested strains of the Paramecium aurelia species complex. All susceptible strains belong to closely related P. primaurelia and P. pentaurelia species. Phylogenetic analysis placed the new species in the Clade 4 of Microsporidia and revealed its close relationship to Euplotespora binucleata (a microsporidium from the ciliate Euplotes woodruffi), to Helmichia lacustris and Mrazekia macrocyclopis, microsporidia from aquatic invertebrates.
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Otter, T., and E. D. Salmon. "Pressure-induced changes in Ca2+-channel excitability in Paramecium." Journal of Experimental Biology 117, no. 1 (July 1, 1985): 29–43. http://dx.doi.org/10.1242/jeb.117.1.29.

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The behaviour of swimming Paramecium is markedly affected by hydrostatic pressure (50–200 atm, 1 atm = 101 325 Pa). To investigate whether pressure might alter behaviour by acting directly on specific ion channels that mediate the behavioural responses, we examined the effects of K+, Na+ and Ba2+ ions on swimming speed and the reversal response during pressurization and decompression. If pressure acted on the channels that transport these ions, then the pressure-induced responses of swimming Paramecium should be exaggerated or diminished, according to which ions were present in the experimental buffer. Pressurization to 100 atm in standard buffer inhibited the brief reversal of swimming direction that occurred at atmospheric pressure when a paramecium encountered the wall of the pressure chamber. To determine whether pressure impaired mechanoreceptor function or directly blocked the Ca2+-channels that control ciliary reversal, we added Ba2+ or Na+ to standard buffer to induce multiple spontaneous reversals. Pressurization blocked these reversals, suggesting that channel opening is directly inhibited by pressure. Decompression in standard buffer elicited momentary ciliary reversal and backward swimming. Buffers with a high ratio of K+ to Ca2+ suppressed this response, and the decompression-induced reversal was exaggerated in the presence of Ba2+ or Na+, consistent with the effects that these ions are known to have on Paramecium's reversal response. These data imply that, upon decompression, the Ca2+-channels that mediate ciliary reversal open transiently. In addition to blocking the reversal response, pressurization slowed forward swimming. By examining the response to pressurization of Paramecium immobilized by Ni2+, we found that hydrostatic pressure apparently slows swimming by reorientating the direction of ciliary beat.
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Melekhin, Maksim, Yulia Yakovleva, Natalia Lebedeva, Irina Nekrasova, Liubov Nikitashina, Michele Castelli, Rosaura Mayén-Estrada, Anna E. Romanovich, Giulio Petroni, and Alexey Potekhin. "Cryptic Diversity in Paramecium multimicronucleatum Revealed with a Polyphasic Approach." Microorganisms 10, no. 5 (May 5, 2022): 974. http://dx.doi.org/10.3390/microorganisms10050974.

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Paramecium (Ciliophora) systematics is well studied, and about twenty morphological species have been described. The morphological species may include several genetic species. However, molecular phylogenetic analyses revealed that the species diversity within Paramecium could be even higher and has raised a problem of cryptic species whose statuses remain uncertain. In the present study, we provide the morphological and molecular characterization of two novel Paramecium species. While Paramecium lynni n. sp., although morphologically similar to P. multimicronucleatum, is phylogenetically well separated from all other Paramecium species, Paramecium fokini n. sp. appears to be a cryptic sister species to P. multimicronucleatum. The latter two species can be distinguished only by molecular methods. The number and structure of micronuclei, traditionally utilized to discriminate species in Paramecium, vary not only between but also within each of the three studied species and, thus, cannot be considered a reliable feature for species identification. The geographic distribution of the P. multimicronucleatum and P. fokini n. sp. strains do not show defined patterns, still leaving space for a role of the geographic factor in initial speciation in Paramecium. Future findings of new Paramecium species can be predicted from the molecular data, while morphological characteristics appear to be unstable and overlapping at least in some species.
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Dissertations / Theses on the topic "Paramecium"

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Gurney, Rebecca L. "Stimulus Generalization to Different levels of Illumination in Paramecium caudatum." Connect to full text in OhioLINK ETD Center, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1228768700.

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Rahemtullah, Shamsa. "Commitment to autogamy in Paramecium blocks mating reactivity : implications for regulation of the sexual pathway and the breeding system." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29779.

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The ciliate Paramecium tetraurelia exhibits two major developmental pathways - the vegetative cell cycle and the sexual pathway. The latter manifests itself in two forms -autogamy (self- fertilization) and conjugation (cross-fertilization) between cells of complementary mating types. In the normal life history young cells are immature for autogamy, but enter conjugation readily. Autogamy first occurs normally at about 20 fissions of age and conjugation disappears by 25. This study documents and analyzes the two major phenomena underlying this unusual life history. It shows how their interaction produces the observed pattern of immaturity, maturity, and senescence (Fig. 1). There are two principal findings of this study. First that commitment to autogamy leads to rapid loss of mating reactivity and second, that there are different starvation thresholds for initiation of mating reactivity and autogamy. The starvation threshold for initiation of mating reactivity is constant, while that for initiation of autogamy decreases progressively as clonal age increases. During the immature period for autogamy the lag between onset of mating reactivity and induction of autogamy decreases with increasing clonal age. The progressive decrease in the starvation threshold required for induction of autogamy brings about the end of the mature period for conjugation. As autogamy is initiated at progressively earlier stages in the growth of a culture, fewer and fewer cells reach the level of starvation required for initiation of mating reactivity prior to induction of autogamy. When the threshold for induction of autogamy becomes so low that no cells develop mating reactivity prior to entering autogamy, the period of maturity for conjugation is over and autogamy becomes the sole sexual process for the remainder of the life history.
Science, Faculty of
Zoology, Department of
Graduate
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Hambach, Kristina. "Klonierung einer Adenylatcyclase aus Paramecium tetraurelia." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964955636.

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Farmakis, Georges. "Recherches sur les ATPases de Paramecium tetraurelia." Grenoble : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb37593820m.

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Watzke, Daniela. "Experimentelle Beeinflussung der gravisensorischen Transduktion bei Paramecium caudatum." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=95898882X.

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Zabolitzky, Andreas. "Bildung und Struktur der intranucleären Mikronucleusspindel des Ciliaten Paramecium bursaria (Focke)." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960695052.

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Schwarz, Steffen. "Untersuchungen zu invasionsspezifischen Proteinen von Holospora obtusa, einem Bakterium aus dem Makronukleus von Paramecium caudatum /." [S.l. : s.n.], 2002. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB9966613.

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Mohamed, Ihab Kamal. "Cell and molecular biological and Fluorochrome analysis of the mechanism involved in the Calcium dynamics during exocytosis in Paramecium cells /." [S.l. : s.n.], 2001. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB9771243.

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Jana, Saikat. "Effect of boundaries on swimming of Paramecium multimicronucleatum." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/51558.

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Microorganisms swimming in their natural habitat interact with debris and boundaries, which can modify their swimming characteristics. However, the boundary effect on swimming microorganisms have not been completely understood yet, and is one of most active areas of research. Amongst microorganisms, unicellular ciliates are the fastest swimmers and also respond to a variety of external cues. We choose Paramecium multimicronucleatum as a model system to understand the locomotion of ciliates. First, we explore the effects of boundaries on swimming modes of Paramecium multimicronu- cleatum by introducing them in 2D films and 1D channels. The geometric confinements cause the Paramecia to transition between: a directed, a meandering and a self-bending behaviors. During the self-bending mode the cell body exerts forces on the walls; which is quantified by using a beam bending analogy and measuring the elasticity of the cell body. The first inves- tigation reveals the complicated swimming patterns of Paramecium caused by boundaries. In the second study we investigate the directed swimming of Paramecium in cylindrical capillaries, which mimics the swimming of ciliates in the pores of soil. A finite-sized cell lo- comoting in extreme confinements creates a pressure gradient across its ends. By developing a modified envelop model incorporating the confinements and pressure gradient effects, we are able to predict the swimming speed of the organisms in confined channels. Finally we study how Paramecium can swim and feed efficiently by stirring the fluid around its body. We experimentally employ "-Particle Image Velocimetry to characterize flows around the freely swimming Parameicum and numerically use Boundary Element Method to quantify the effect of body shapes on the swimming and feeding process. Results show that the body shape of Paramecium (slender anterior and bulky posterior) is hydrodynamically optimized to swim as well as feed efficiently. The dissertation makes significant advances in both experimentally characterizing and the- oretically understanding the flow field and locomotion patterns of ciliates near solid bound- aries.
Ph. D.
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Fisch, Cathy. "Functional analysis of B9-domain proteins in paramecium." Paris 11, 2009. http://www.theses.fr/2009PA112219.

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Les cils sont des organites eucaryotes dont la structure et les fonctions mobiles et sensorielles sont ou ont conservées des organismes unicellulaires à l’homme. L’importance de ces organites s’est manifestée en découvrant que différents syndromes génétiques comme les polykystoses rénales, les syndromes de Bardet-Biedl, de Meckel-Gruber ou de Joubert (pathologies caractérisées par des défauts développementaux du rein, du cerveau et des membres) sont causés par la perte des fonctions ciliaires. Par la suite, l’analyse protéomique du complexe corps basal/cil et la caractérisation génétique des ciliopathies humaines a permis l’identification de nombreuses nouvelles protéines dont les fonctions cellulaires restent à élucider. En utilisant Paramécium tetraurelia, modèle eucaryote unicellulaire cilié, nous avons caractérisé une famille de protéines à trois membres (MKS1, EPPB9 , et ICIS) qui est impliquée dans les ciliopathies. La localisation de ces protéines au niveau du cil est typique de protéines de la ciliogenèse. Nos analyses d’ARN interférence ont montré que ces protéines étaient essentielles à la survie cellulaire et à la stabilité ciliaire. La suppression de ces protéines interfère avec l’activité sensorielle et la stabilité structurale du cil. L’analyse ultrastructurale montre également de graves défauts de maturation des vésicules de transport, vésicules alimentant le cil en protéines. L’ensemble de nos résultats démontre un rôle fondamental de ces protéines dans le transport vésiculaire vers la membranes ciliaire
Cilia are eukaryotic organelles whose structure and functions in motility and sensation are conserved from unicellular organisms to human. The importance of those organelles has recently come into focus with the discovery that various human genetic syndromes, such as polycystic kidney disease, Bardet-Biedl, Meckel of Joubert syndrome, which are charaterized by the association of developmental defects in the central nervous system and limbs, are caused by the loss of ciliary funtions. Proteomic analysis of the basal body/cilium complex and the genetic characterization of human syndromes identified numerous new proteins that are evolutionary conserved but whose cellular functions remain to be elucidated. Taking advantage of a eukaryotic unicellular ciliated model organism, Paramecium tetraurelia, we characterised a protein family of three members (MKS1, EPPB9 and ICIS) which are implicated in ciliopathies. Localisation of these proteins at the cilium supported their potential role in ciliogenesis. RNA interference analysis showed that these proteins are essential for cell survival and ciliary stability. Their absence caused severe sensorial dysfunction followed by the progressive loss of cilia. Ultrastructural analysis showed that the maturation process of exocytotic vesicles was inhibited. Taken together these results suggest a fundamental role for proteins in the vesicular traffic to the apical cell membrane and targeting to the ciliary membrane
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Books on the topic "Paramecium"

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Görtz, Hans-Dieter, ed. Paramecium. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73086-3.

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Beale, Geoffrey. Paramecium. London: Taylor and Francis, 2008.

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Fujishima, Masahiro, ed. Endosymbionts in Paramecium. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-92677-1.

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service), SpringerLink (Online, ed. Endosymbionts in Paramecium. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2009.

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Wichterman, Ralph. The Biology of Paramecium. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4757-0372-6.

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Beale, Geoffrey. Paramecium: Genetics and epigenetics. Boca Raton: CRC Press, 2008.

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Lüthe, Norbert. Glykoproteinnachweis bei Paramecium tetraurelia. Konstanz: Hartung-Gorre, 1987.

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1918-, Preer John R., ed. Paramecium: Genetics and epigenetics. Boca Raton: CRC Press, 2008.

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I, Rybakova Z., and Dubinin Nikolaĭ Petrovich 1907-, eds. Genetika infuzoriĭ: Tetrahymena i Paramecium. Moskva: Izd-vo "Nauka", 1986.

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Takagi, Yoshiomi. Yūsei seishokuron: "sei" to "shi" wa naze umareta no ka. Tōkyō-to Shibuya-ku: NHK Shuppan, 2014.

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Book chapters on the topic "Paramecium"

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Görtz, Hans-Dieter. "Introduction." In Paramecium, 1–3. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73086-3_1.

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Freiburg, Manfred. "Organization and Expression of the Nuclear Genome." In Paramecium, 141–54. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73086-3_10.

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Schmidt, Helmut J. "Immobilization Antigens." In Paramecium, 155–66. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73086-3_11.

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Sainsard-Chanet, Annie, and Donald Cummings. "Mitochondria." In Paramecium, 167–84. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73086-3_12.

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Machemer, Hans. "Electrophysiology." In Paramecium, 185–215. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-73086-3_13.

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Machemer, Hans. "Motor Control of Cilia." In Paramecium, 216–35. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-73086-3_14.

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Ramanathan, Rajeev, Yoshiro Saimi, Robert Hinrichsen, Anthony Burgess-Cassler, and Ching Kung. "A Genetic Dissection of Ion-Channel Functions." In Paramecium, 236–53. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-73086-3_15.

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Schultz, Joachim E., and Susanne Klumpp. "Biochemistry of Cilia." In Paramecium, 254–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-73086-3_16.

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Takahashi, Mihoko. "Behavioral Genetics in P. caudatum." In Paramecium, 271–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-73086-3_17.

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Van Houten, Judith, and Robin R. Preston. "Chemokinesis." In Paramecium, 282–300. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-73086-3_18.

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Conference papers on the topic "Paramecium"

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Hayasaka, Nozomi, Takumi Kikuchi, and Akitoshi Itoh. "Transportation Work of Gel Object Done by a Paramecium in the Vertical Plane Pool." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-71301.

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We have been investigating how to use microorganisms for bio-micromachines. In this paper, we investigated the motion control property of Paramecium in the vertical plane to prepare the real 3-dimensional motion control. First, we developed a motion control pool for the vertical set up. Basically, the controllability of Paramecium in the vertical plane is not so different to the controllability in the horizontal plane. We can control paramecium very stably for over 100 laps along the star-shaped target route by using this newly made experimental pool. The controllability was improved with the progression of making a circuit. It may relate to the dropping of the swimming speed. The swimming trace, however, showed the peculiarity that related to the vertical movements. The swimming speed of the downward direction is higher than that of the upward direction. The overrun on the downward route was larger than that on the upward route in the vertical plane. It was caused by the difference of the swimming speed on each of routes. Therefore, we developed a new motion control algorithm to decrease this overrun. In our former algorithm, the change timing of the target point was decided by the previous change timing and the previous turning point. In the new algorithm, we change this adjusting method to refer the same target point of the past laps using smoothing value calculated by the integral of the equal ratio attenuation. By using this adjustment method, we succeeded to decrease the overrun. We also investigated the transportability of the object by using motion controlled paramecium in the vertical pool. We found that paramecia often cause their avoiding reaction when they hit object made of hard material. In the case of the object made of soft material, paramecia can push more often and more easily. Therefore, we decided to change the target object from hard plastic to soft gel. We succeeded to transport and drop a gel oval sphere to the target place by manually controlled paramecium in the vertical plane pool.
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Du, Qing, Shifang Li, Edward S. Fry, and Karl Aufderheide. "Laser tweezer manipulation of micronuclei in Paramecium." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1991. http://dx.doi.org/10.1364/oam.1991.wg5.

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Optical tweezers are used in Paramecium to control the positions of nuclei that play an important role during sexual reorganization. The beam from a Nd:YAG laser (λ = 1060 nm) was directed through a beam expanding telescope, through the microscope, and onto the specimen. With a 100× planachromatic phase contrast objective, ~100 mW could be focused into a 2-3-μm spot. First, the living Paramecium was immobilized in a rotocompressor. As the result of radiation pressure at the diffraction-limited focus of the laser beam, organelles in these immobilized Paramecium could be easily trapped and manipulated. The structures that could be most easily trapped and moved were the crystals. On the other hand, with available power levels we were not able to directly trap the micro- and macronuclei because of their small relative index of refraction. However, trapped crystals could be moved virtually anywhere in the cell at the observer’s discretion, and it was demonstrated that they could be used to push the micronuclei around the cell at will. Moreover, the growth and reproduction of Paramecia that had undergone laser irradiation at our available power levels showed no deleterious effects and showed no differences with non-irradiated control specimens.
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Bernal-Martínez, Juan. "L-glutamate Receptor In Paramecium." In MEDICAL PHYSICS: Eighth Mexican Symposium on Medical Physics. AIP, 2004. http://dx.doi.org/10.1063/1.1811853.

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Toderas, Ion, Aurelian Gulea, Valentin Gudumac, Elena Roscov, and Olga Garbuz. "Metodă nouă de apreciere a toxicităţii substanţelor chimice." In International symposium ”Functional ecology of animals” dedicated to the 70th anniversary from the birth of academician Ion Toderas. Institute of Zoology, Republic of Moldova, 2019. http://dx.doi.org/10.53937/9789975315975.81.

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We developed a new invention that relates to method for assessing the toxicity of chemical substances.The method of the invention includes the preparation of the culture of Paramecium caudatum, adding to the samples investigated to test chemicals in various concentrations, incubating the sample to be studied and control the addition of colourant 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, incubation with subsequent addition of solution of formalin, centrifuging the sample, removing the supernatant, adding the solution of hydroxide of sodium, determine the absorbance using a spectrophotometer, followed by calculating the percentage of paramecii viability and determine the lethal concentration (LC50) at the same time as the value of the concentration LC50 is small, the toxicity of the tested substance is higher.
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Strilets, O., and L. Strelnikov. "VERWENDUNG VON PARAMECIUM CAUDATUM BEI BIOTESTING." In TENDENZE ATTUALI DELLA MODERNA RICERCA SCIENTIFICA. European Scientific Platform, 2020. http://dx.doi.org/10.36074/05.06.2020.v2.51.

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Yamaguchi, Yuuki. "Large volume 3D EDS Mapping of Paramecium." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.751.

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Bernal-Martínez, Juan. "Pb2+ Modulates Ca2+ Membrane Permeability In Paramecium." In MEDICAL PHYSICS: Eighth Mexican Symposium on Medical Physics. AIP, 2004. http://dx.doi.org/10.1063/1.1811852.

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Grishacheva, T. G., D. R. Faizullina, A. R. Muslimov, L. V. Chistyakova, E. A. Korneva, and N. N. Petrishchev. "Different photosensitizers distribution in Paramecium caudatum Ehrenberg, 1838." In Clinical and Translational Biophotonics. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/translational.2020.jtu3a.27.

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Hirano, Akira, Toshio Tsuji, Noboru Takiguchi, and Hisao Ohtake. "An electrophysiological model of chemotactic response in Paramecium." In 2006 IEEE International Conference on Systems, Man and Cybernetics. IEEE, 2006. http://dx.doi.org/10.1109/icsmc.2006.384690.

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MELEKHIN, M., L. NIKITASHINA, N. LEBEDEVA, G. PETRONI, O. LANZONI, I. NEKRASOVA, S. I. FOKIN, and A. POTEKHIN. "IS PHYLOGENY BLIND WITHOUT MORPHOLOGY? THE CASE OF PARAMECIUM." In 5TH MOSCOW INTERNATIONAL CONFERENCE "MOLECULAR PHYLOGENETICSAND BIODIVERSITY BIOBANKING". TORUS PRESS, 2018. http://dx.doi.org/10.30826/molphy2018-58.

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