Relevant bibliographies by topics / Patch de la membrane

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Academic literature on the topic 'Patch de la membrane'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 February 2022

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Journal articles on the topic "Patch de la membrane"

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Garten, Matthias, Lars D. Mosgaard, Thomas Bornschlögl, Stéphane Dieudonné, Patricia Bassereau, and Gilman E. S. Toombes. "Whole-GUV patch-clamping." Proceedings of the National Academy of Sciences 114, no. 2 (December 21, 2016): 328–33. http://dx.doi.org/10.1073/pnas.1609142114.

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Studying how the membrane modulates ion channel and transporter activity is challenging because cells actively regulate membrane properties, whereas existing in vitro systems have limitations, such as residual solvent and unphysiologically high membrane tension. Cell-sized giant unilamellar vesicles (GUVs) would be ideal for in vitro electrophysiology, but efforts to measure the membrane current of intact GUVs have been unsuccessful. In this work, two challenges for obtaining the “whole-GUV” patch-clamp configuration were identified and resolved. First, unless the patch pipette and GUV pressures are precisely matched in the GUV-attached configuration, breaking the patch membrane also ruptures the GUV. Second, GUVs shrink irreversibly because the membrane/glass adhesion creating the high-resistance seal (>1 GΩ) continuously pulls membrane into the pipette. In contrast, for cell-derived giant plasma membrane vesicles (GPMVs), breaking the patch membrane allows the GPMV contents to passivate the pipette surface, thereby dynamically blocking membrane spreading in the whole-GMPV mode. To mimic this dynamic passivation mechanism, beta-casein was encapsulated into GUVs, yielding a stable, high-resistance, whole-GUV configuration for a range of membrane compositions. Specific membrane capacitance measurements confirmed that the membranes were truly solvent-free and that membrane tension could be controlled over a physiological range. Finally, the potential for ion transport studies was tested using the model ion channel, gramicidin, and voltage-clamp fluorometry measurements were performed with a voltage-dependent fluorophore/quencher pair. Whole-GUV patch-clamping allows ion transport and other voltage-dependent processes to be studied while controlling membrane composition, tension, and shape.
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Sokabe, M., and F. Sachs. "The structure and dynamics of patch-clamped membranes: a study using differential interference contrast light microscopy." Journal of Cell Biology 111, no. 2 (August 1, 1990): 599–606. http://dx.doi.org/10.1083/jcb.111.2.599.

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We have developed techniques for micromanipulation under high power video microscopy. We have used these to study the structure and motion of patch-clamped membranes when driven by pressure steps. Patch-clamped membranes do not consist of just a membrane, but rather a plug of membrane-covered cytoplasm. There are organelles and vesicles within the cytoplasm in the pipette tip of both cell-attached and excised patches. The cytoplasm is capable of active contraction normal to the plane of the membrane. With suction applied before seal formation, vesicles may be swept from the cell surface by shear stress generated from the flow of saline over the cell surface. In this case, patch recordings are made from membrane that was not originally present under the tip. The vesicles may break, or fuse and break, to form the gigasealed patch. Patch membranes adhere strongly to the wall of the pipette so that at zero transmural pressure the membranes tend to be normal to the wall. With transmural pressure gradients, the membranes generally become spherical; the radius of curvature decreasing with increasing pressure. Some patches have nonuniform curvature demonstrating that forces normal to the membrane may be significant. Membranes often do not respond quickly to changes in pipette pressure, probably because viscoelastic cytoplasm reduces the rate of flow through the tip of the pipette. Inside-out patches may be peeled from the walls of the pipette, and even everted (with positive pressure), without losing the seal. This suggests that the gigaseal is a distributed property of the membrane-glass interface.
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McNeil, P. L., S. S. Vogel, K. Miyake, and M. Terasaki. "Patching plasma membrane disruptions with cytoplasmic membrane." Journal of Cell Science 113, no. 11 (June 1, 2000): 1891–902. http://dx.doi.org/10.1242/jcs.113.11.1891.

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Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca(2+) can rapidly erect large membrane boundaries. These might be used as a ‘patch’ for resealing plasma membrane disruptions. Three central predictions of this ‘patch’ hypothesis are here established in sea urchin eggs. First, we show that surface markers for plasma membrane protein and lipid are initially absent over disruption sites after resealing is complete. Second, we demonstrate that resealing capacity is strongly dependent upon local availability of fusion competent cytoplasmic organelles, specifically the reserve or yolk granule. Lastly, we demonstrate that the reserve granule is capable of rapid (t(1/2) <1 second), Ca(2+)-regulated (high threshold) fusion capable of erecting large (>1000 μm(2)), continuous membrane boundaries. Production of patch vesicles for resealing may proceed by an ‘emergency’ fusion mechanism distinct from that utilized for the much slower, highly regulated, cytosol-requiring organelle-organelle fusion events typical of constitutive membrane trafficking pathways.
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Tedeschi, Henry, Carmen A. Mannella, and Charles L. Bowman. "Patch clamping the outer mitochondrial membrane." Journal of Membrane Biology 97, no. 1 (February 1987): 21–29. http://dx.doi.org/10.1007/bf01869611.

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Minami, Kunihiro, and Yoshihiro Iwao. "The Formulation Design of Transdermal β1–blocker Patch （“Bisono®Tape”）." membrane 41, no. 2 (2016): 87–92. http://dx.doi.org/10.5360/membrane.41.87.

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Engbretson, B. G., K. W. Beyenbach, and L. C. Stoner. "The everted renal tubule: a methodology for direct assessment of apical membrane function." American Journal of Physiology-Renal Physiology 255, no. 6 (December 1, 1988): F1276—F1280. http://dx.doi.org/10.1152/ajprenal.1988.255.6.f1276.

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A technique is described whereby it is possible to evert a 0.5- to 1.0-mm segment of an amphibian renal tubule and perfuse it in vitro. Consequently, the apical membranes of an intact nephron fragment are directly accessible for electrophysiological study. Viability of the cells of everted diluting segments taken from Ambystoma kidney was indicated by 1) failure of the cells to take up trypan blue and 2) the existence of an apical membrane voltage (average 66 mV, cell negative), which decreased predictably in the presence of either 5 mM barium or elevated potassium in the luminal bathing solution. The utility of the everted tubule to patch clamp studies was tested. A large conductance channel that appeared to be selective for potassium could be demonstrated in a cell-attached patch of the apical membrane of an everted initial collecting tubule. The everted tubule preparation not only provides large quantities of apical membrane for patch clamp studies but, more importantly, allows the investigator to control the solutions bathing each membrane surface independently. The application of patch clamp techniques to perfused, everted tubules may then serve to more completely describe the role of the apical membrane in transcellular ion transport.
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Waqar, Tariq, Yasir Khan, and Muhammad Usman Riaz. "SUBAORTIC MEMBRANE;." Professional Medical Journal 24, no. 12 (November 29, 2017): 1801–5. http://dx.doi.org/10.29309/tpmj/2017.24.12.612.

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Objectives: In this study, we presented our results regarding outcomes ofsurgical correction of sub-aortic membrane. Study Design: Retrospective observational study.Period: June 2012 to June 2017. Setting: CPEIC Multan, Pakistan. Methods: 51 patientsoperated for resection of sub aortic membrane. The resection of sub aortic membrane wasdone through the aorta. Evaluation of the aortic valve done in all patients. The aortic valve waseither replaced or repaired in cases of severe aortic regurgitation. Associated lesions such asventricular septal defects (VSD’s) were repaired with a dacron patch through the right atriumwhile ASD’s were repaired with a pericardial patch. Post-operative echocardiography was donebefore discharge and post-op LVOT gradients and aortic insufficiency were recorded for allthe patients. Results: There were 36 males and 15 females whose mean ages were 16.29years. On post-op echocardiography there was no residual significant LVOT gradient in anypatient. Three (3) patients developed mild to moderate aortic regurgitation post operativelybut none of them warrant any surgical intervention. There was only 1 death in the series whichwas due to VSD patch dehiscence. None of the patients developed conduction problems postoperatively needing any permanent pace maker. Mean pre-op LVOT gradient was 94.7 mmHgwhile it reduced to 20.7 post operatively (p-value <0.001). Conclusion: We concluded thatearly resection of sub aortic membrane can be safely accomplished with good results andsignificant drop in the mean LVOT pressure gradients post operatively.
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Hair, Philip I., Gillian M. Keating, and Kate McKeage. "Transdermal Matrix Fentanyl Membrane Patch (Matrifen®)." Drugs 68, no. 14 (2008): 2001–9. http://dx.doi.org/10.2165/00003495-200868140-00005.

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Glover, Louise, and Robert H. Brown. "Dysferlin in Membrane Trafficking and Patch Repair." Traffic 8, no. 7 (June 5, 2007): 785–94. http://dx.doi.org/10.1111/j.1600-0854.2007.00573.x.

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Ruknudin, A., M. J. Song, and F. Sachs. "The ultrastructure of patch-clamped membranes: a study using high voltage electron microscopy." Journal of Cell Biology 112, no. 1 (January 1, 1991): 125–34. http://dx.doi.org/10.1083/jcb.112.1.125.

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We have developed techniques for studying patch-clamped membranes inside glass pipettes using high voltage electron microscopy (HVEM). To preserve the patch structure with the least possible distortion, we rapidly froze and freeze dried the pipette tip. The pipette is transparent for more than 50 microns from the tip. HVEM images of patches confirm light microscopy observations that the patch is not a bare bilayer, but a membrane-covered bleb of cytoplasm that may include organelles and cytoskeleton. The membrane that spans the pipette is commonly tens of micrometers from the tip of the pipette and occasionally as far as 100 microns. The structure of patches taken from a single cell type is variable but there are consistent differences between patches made from different cell types. With suction applied to the pipette before seal formation, we have seen in the light microscope vesicles swept from the plasmalemma up the pipette. These vesicles are visible in electron micrographs, particularly those made from chick cardiac muscle. Colloidal gold labeling of the patch permitted identification of lectin-binding sites and acetylcholine receptors. In young cultures of Xenopus myocytes, the receptors were diffuse. In 1-wk-old cultures, the receptors formed densely packed arrays. The patch pipette can serve, not only as a recording device, but as a tool for sampling discrete regions of the cell surface. Because the pipette has a constant path length for axial rotation, it is a unique specimen holder for microtomography. We have made preliminary tomographic reconstructions of a patch from Xenopus oocyte.
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Dissertations / Theses on the topic "Patch de la membrane"

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Squire-Pollard, Laura G. "A patch-clamp study of membrane ion channels in exocrine acinar cells." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316552.

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Marsh, Katherine M., Alice S. Ferng, Tia Pilikian, Ankit A. Desai, Ryan Avery, Mark Friedman, Isabel Oliva, Clint Jokerst, David Schipper, and Zain Khalpey. "Anti-inflammatory properties of amniotic membrane patch following pericardiectomy for constrictive pericarditis." BIOMED CENTRAL LTD, 2017. http://hdl.handle.net/10150/622952.

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Background: Since constrictive pericarditis is most often idiopathic and the pathophysiology remains largely unknown, both the diagnosis and the treatment can be challenging. However, by definition, inflammatory processes are central to this disease process. Amniotic membrane patches have been shown to possess anti-inflammatory properties and are believed to be immune privileged. Due to these properties, amniotic membrane patches were applied intraoperatively in a complicated patient presenting with constrictive pericarditis. Case presentation: A patient with a history of multiple cardiac surgeries presented with marked fatigue, worsening dyspnea and sinus tachycardia. He was found to have constrictive physiology during cardiac catheterization, with cardiac MRI demonstrating hepatic vein dilatation, atrial enlargement and ventricular narrowing. After amniotic membrane patch treatment and pericardiectomy, post-operative cardiac MRI failed to demonstrate any appreciable pericardial effusion or inflammation, with no increased T2 signal that would suggest edema. Conclusions: Given the positive results seen in this complex patient, we suggest continued research into the beneficial properties of amniotic membrane patches in cardiac surgery.
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Williams, Robert Brett. "Localized Effects of Piezopolymer Devices on the Dynamics of Inflatable Space-Based Structures." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/34542.

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Inflatable space-based devices have become popular over the past three decades, as they offer minimized launch-mass and launch-volume. Since some satellites have mirror sections over fifty feet in diameter and struts with lengths over ninety feet, inflation while in orbit has become a necessary procedure. Once inflated, these space structures are subject to two types of vibrations: those induced mechanically by guidance systems and space debris and those induced thermally from variable amounts of direct sunlight as they orbit about earth. Controlling vibrations of spaced-based structures is critical to ensuring optimal performance. The focus of this research is derived from an Air Force program to develop and model an active control system using smart materials to suppress the vibrations of inflatable communication satellites. When small piezoceramic devices are attached to an aluminum or steel structure, the effects of the piezo on the dynamic properties of the host are typically ignored. However, the inflatable satellites of interest to this project are manufactured from Kapton®, a thin, light polyimide film. Therefore, even a piezopolymer film actuator, such as PVDF, could greatly change the mass and stiffness values in the area under and around the patch, altering the dynamic behavior of the satellite.

Thin-walled pressure vessel theory was employed to assess the state of stress at any location on an inflated torus. A flat, rectangular coupon was selected at a general point on the structure and modeled as a membrane. The equation of motion for this membrane with clamped edges was derived and a closed-form solution for the natural frequencies and mode shapes was presented. The Rayleigh-Ritz and finite element methods were then seen to numerically approximate the natural frequencies and mode shapes for the bare membrane with a high degree of accuracy. A passive PVDF patch was then attached to the base membrane and the equation of motion derived using an energy approach. Since a closed-form solution was not readily available, the Rayleigh-Ritz and finite element methods were again employed to obtain approximate results that agreed remarkably well. Trends in natural frequencies for various patch areas and thicknesses were explored. It was shown, that membrane theory represented the added mass of the patch but was unable to account for the added stiffness of the PVDF attachment. Traditional membrane theory was also unable to model an active PVDF patch as a sensor for out of plane vibrations, but the ability of the patch to alter the tension in the base layer was predicted.
Master of Science

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Pilsudski, Richard. "Contribution à l'étude des conductances potassiques de la membrane cardiaque." Lyon 1, 1989. http://www.theses.fr/1989LYO10137.

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Les canaux potassiques participent au controle du potentiel d'action membranaire et de l'excitabilite de la cellule musculaire cardiaque. Afin de mieux comprendre les mecanismes de la regulation de ces canaux nous avons realise des experiences de patch-clamp dans la configuration cellule entiere sur des cellules isolees d'oreillette et de ventricule de grenouille. Nous avons montre que l'application d'acetylcholine (ach) sur la face externe de la membrane plasmique ou l'application de gtp gamma s sur la face interne de cette membrane induisent l'activation d'un courant potassique i#k#(#a#c#h#) qui pourrait etre different du courant potassique de repos i#k#1. L'application d'adenosine n'entraine aucune modification des conductances potassiques. Sur les cellules cardiaques un courant potassique i#k(atp) s'active lorsque le contenu en adenosine triphosphate est faible. Nous avons montre que nous pouvions activer un courant semblable sur les cellules auriculaires de grenouille par dialyse avec un milieu intracellulaire contenant 40 m de guanosine diphosphate ou par perfusion par un milieu extra-cellulaire exempt de substrats energetiques tels que le glucose. Ce courant i#k#(#a#t#p) peut aussi etre active sur notre preparation par l'application externe de cromakalim. Cette activation a lieu malgre une forte teneur en atp intracellulaire. Nos differents resultats indiquent que la cellule musculaire isolee de cur de grenouille est un outil ideal d'etude des conductances potassiques cardiaques et de leur modes multiples de regulation
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Vaheb, Yaser. "Assembly of molecular nanomagnets into nanogap electrodes by dielectrophoresis. Realization of bioelectronic devices for electrical measurement of ionic current through membrane protein channels." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112320.

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Cette thèse se compose de deux parties qui peuvent être considérées comme deux aspects différents de l'électronique moléculaire avec pour point commun les moyens de nanofabrication mis en jeu pour réaliser des dispositifs de mesures électriques à bas courant. La première partie de la thèse concerne l'assemblage de nano-aimants entre électrodes à nanogap. Le besoin croissant de processeurs toujours plus performants et celui d’une densité de stockage toujours plus grande ont poussé la technologie CMOS couramment utilisée dans l'industrie à ses limites physiques vis-à-vis de sa miniaturisation. L'électronique moléculaire et la spintronique moléculaire se révèlent être des alternatives prometteuses à cette technologie pour les futurs dispositifs nanoélectroniques. Mes principaux travaux dans ce domaine ont porté sur l'assemblage entre des électrodes à nanogap, de nano-aimants moléculaires à base de bleu de Prusse ou de son analogue Cs–Co–Cr. Le but était ainsi de faire les premiers pas vers la construction de dispositifs en spintronique moléculaire. Des nanogaps de ~ 7 à 50 nm ont été fabriqués en palladium ou en or sur un substrat Si/SiO₂ par lithographie électronique et lift-off. Les nano-aimants ont été placés dans le gap par diélectrophorèse à courant alternatif (AC DEP). À température ambiante, un courant négligeable a été mesuré sur les jonctions utilisant des nanoparticules de Cs–Co–Cr alors qu’un courant de ~ 30 pA a été mesuré sur celles avec les nanoparticules en bleu de Prusse pour une tension de ~ 1 V. J’ai montré qu‘en fait, l’eau piégée dans les nanogaps altérait sérieusement les mesures de courants et nécessitait un recuit préalable. Pour optimiser la localisation des nanoparticules entre les électrodes, j’ai proposé un programme de simulation de la DEP ne tenant pas compte du mouvement brownien et de la dynamique des fluides. La deuxième partie de la thèse concerne la fabrication de dispositifs de type nanopatch-clamp planaire pour l'enregistrement de courants ioniques à travers les canaux ioniques des protéines membranaires. Les canaux de ces protéines incorporées dans les membranes cellulaires sont des composantes essentielles de toutes les cellules vivantes et sont à la base de divers processus physiologiques tels que ceux dans la communication nerveuse, la contraction musculaire, la sensation tactile, etc. Les mesures de transport d'ions sont maintenant utilisées dans diverses applications telles que le criblage de médicaments dans l'industrie pharmaceutique et les biocapteurs médicaux. La méthode classique pour effectuer des mesures de transport d'ions consiste à utiliser un système patch-clamp. Cependant, cette méthode nécessite d’importantes compétences, des équipements lourds et coûteux et présente une faible efficacité de mesure. Pour pallier ces inconvénients, une solution est de développer des patch-clamps planaires, qui sont modulables, automatisés et faciles d’utilisation. La fabrication du dispositif a consisté en la réalisation d’une piste conductrice constituée d’un empilement de couches Au/Ag sur un substrat de silicium oxydé. Cette piste a été passivée et isolée électriquement par une couche de Si₃N₄/SiO₂ dans laquelle j’ai gravé des micro-trous et j’ai ensuite converti la couche d’Ag en AgCl pour les mesures électriques. Afin de valider le fonctionnement du dispositif sans la membrane, j’ai procédé à des mesures de courant en fonction du temps pour diverses tensions, ce qui m’a ensuite permis de proposer un schéma équivalent électrique
This thesis consists of two parts. The two parts correspond to two different subjects but with a common feature which is the fabrication of nanometer scale devices for low current measurements. The first part investigated the assembly of Prussian blue and Cs–Co–Cr Prussian blue analogue molecular nanomagnets into nano-patterned electrodes. The ever growing need for higher performance processors and higher storage densities has pushed the CMOS technology commonly used in industry to its physical limitations toward its miniaturization. Molecular electronics and molecular spintronics prove to be promising alternatives for the CMOS in future nanoelectronic devices. Pd or Au gaps with ~ 7–50 nm width were fabricated on a Si/SiO₂ substrate using standard electron beam lithography, metal deposition and lift-off. Nanomagnets were positioned between the gaps via AC dielectrophoresis (DEP). At room temperature, the Cs–Co–Cr Prussian blue analogue nanoparticles exhibited negligible current whereas junction with Prussian blue nanoparticles exhibited ~ 30 pA at ~ 1 V. Water trapped in nanogaps was found to seriously alter current measurements. This problem was solved by heating samples prior to measurements. A simplified DEP simulation program using Delphi was developed, which neglected Brownian motion and fluid dynamics but allowed us to better understand the DEP process. The second part of the thesis investigated the fabrication of devices for measuring electrical currents through membrane protein channels. Membrane-embedded protein channels are the basis of various physiological processes like nervous communication, muscular contraction, tactile sensation, and so on. Electrical measurements are used in different applications such as drug screening in pharmaceutical industry and biosensors. The standard method to perform such measurements is the use of patch-clamp. However, this method requires intense skill and heavy equipment while it exhibits low measurement efficiency. A solution to these drawbacks is the development of planar patch clamps, which are scalable, automated and easier to use. The first device fabrication step was the patterning of Au/Ag electrodes on thermally oxidized Si substrate by optical lithography, metallization and lift-off. Secondly, a passivation layer of Si₃N₄/SiO₂ was deposited on top of electrodes by PECVD. Then micro-holes were formed inside the Si₃N₄/SiO₂ passivation layer stack using Raith-150 e-beam lithography and reactive ion etching. Finally, Ag layer was converted to AgCl using bleach. The test of electrical current was done using Axopatch patch-clamp amplifier. Current versus time measurements for different voltages were recorded without membrane covering the holes, and an electrical model has been developed for the fabricated devices
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Chen, Chih-chen. "Microfluidic elastomeric platforms for probing single cells /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/8029.

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Lourdel, Stéphane. "Etude des canaux potassium et chlorure de la membrane basolatérale du tubule distal de souris." Paris 7, 2003. http://www.theses.fr/2003PA077070.

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Lapaix, Franck. "Le parasite de la malaria (paludisme) active des canaux ioniques endogènes de la membrane des globules rouges." Rennes 1, 2004. http://www.theses.fr/2004REN1S155.

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Le développement rapide de la résistance aux anti-paludiques et l'extension rapide des zones de prévalence de la malaria imposent de mettre au point de nouvelles approches thérapeutiques. Le pouvoir de résolution des techniques de l'électrophysiologie permet aujourd'hui d'envisager une stratégie nouvelle qui consiste à identifier les canaux ioniques utilisés par le parasite dans la membrane de la cellule hôte (globule rouge), pour l'acquisition des nutriments essentiels pour sa multiplication et le rejet de ses déchets métaboliques pendant la phase intraérythrocytaire de son cycle vital. Ces voies constituent des cibles potentielles pour une inhibition sélective ou, au contraire pour permettre le passage de nouvelles molécules thérapeutiques. Cet axe de recherche est développé dans le but de fournir de nouveaux agents pour la chimiothérapie de la malaria. Les résultats de cette thèse montrent pour la première fois l'existence de canaux anioniques dans la membrane des éryhtrocytes humains et leur rôle physiologique restera à déterminer. Ils apportent également des informations importantes concernant les modifications induites par le parasite dans la membrane de la cellule hôte pour y permettre sa survie. Il est évident désormais que le parasite détourne à son profit des canaux natifs de la membrane érythrocytaire.
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Bouyer, Guillaume. "Caractérisation des canaux anioniques dans la membrane du globule rouge humain infecté par Plasmodium falciparum." Rennes 1, 2009. http://hal.upmc.fr/tel-01105244v1.

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La perméabilité membranaire de l’érythrocyte humain est fortement modifiée au cours de l’infection par Plasmodium falciparum, et de nouvelles voies de perméabilités (NPPs) ayant des propriétés de canaux anioniques apparaissent. Des canaux sont actifs dans la membrane de l’érythrocyte infecté, néanmoins les données de la littérature divergent sur le nombre et la nature de ces canaux. Les premiers résultats de cette thèse montrent l’activité de deux types de canaux endogènes, l’IRC et l’ORC, lors de l’infection par Plasmodium falciparum. Un troisième type de canal, le SCC, a également pu être caractérisé. Par la suite, une caractérisation de l’activité de ces canaux a montré que le SCC était semblable au canal PSAC décrit dans des conditions supra-physiologiques; et qu’il s’agissait vraisemblablement d’un canal endogène de type ClC-2. Par ailleurs, si l’activité spontanée de l’ORC ne semble pas modifiée lors de l’infection, l’IRC est activé par le parasite et son comportement au niveau unitaire est modifié en la présence de sérum, expliquant les modifications observées sur la conductance globale. Enfin, dans la dernière partie de cette étude, des données suggérant l’implication d’un complexe endogène de type PBR (Peripheral Benzodiazepine Receptor) dans le phénomène des NPPs ont été accumulées. Dans cette hypothèse, l’IRC ne serait alors qu’un sous-état d’un canal anionique de conductance plus importante. Ce travail permet donc de mieux comprendre les données électrophysiologiques sur les canaux anioniques dans la membrane de l’érythrocyte infecté, et propose une nouvelle hypothèse quant à la nature des voies de perméabilité impliquées dans les NPPs
Plasmodium falciparum alters the permeability of its host erythrocyte plasma membrane, inducing the formation of New Permeation Pathways (NPPs). These NPPs have been shown to have anion channel properties, and anion channels are active in the membrane of infected cells. Nevertheless conflicting data exist, regarding the number, nature and origin of these anion channels. In the present study, we could show the activity of two types of endogenous channels, IRC and ORC, in the infected red blood cell membrane. A third new type of channel, SCC, was also active in these cells. Further characterization of the channels activity demonstrated that SCC is the correlate of PSAC, a channel described in supra-physiological conditions, and that this channel could be an endogenous ClC-2 channel. Moreover, if ORC activity does not seem to be modified during infection, IRC is activated by the parasite and its behaviour is modified in presence of serum. Thus this could potentially explain the alteration of global conductance seen in the presence of serum on infected cell membrane. In the last part of this study, data suggesting the implication in the NPPs of the components of an endogenous PBR complex (Peripheral Benzodiazepine Receptor) were accumulated. This hypothesis could imply that the IRC seen in the membrane of infected cells is a substate of a large anion channel with multiple possible conductances. To summarize, this work brings some new elements for the comprehension of electrophysiological data obtained on the membrane of Plasmodium-infected erythrocytes, and proposes a new hypothesis regarding the nature of the pathways involved in the NPPs
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Nissant, Antoine. "Caractérisation des canaux chlorures de la membrane basolatérale du néphron distal de souris." Paris 6, 2005. http://www.theses.fr/2005PA066450.

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Books on the topic "Patch de la membrane"

1

ill, Papp Lisa, ed. Patch. New York: Scholastic, 2006.

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Duey, Kathleen. Patch. Carlsbad, CA: Big Guy Books, 2002.

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Flatres, Peter. Patch. St. Martin's, Guernsey: Burbridge, 1998.

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Gunn, Thom. Patch work. [Chicago: Modern Poetry Association, 1987.

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Cox, Maria Victoria Aguirre. The patch. San Antonio, Tex: Victoria's Ink, 2005.

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Headley, Justina Chen. The patch. Watertown, MA: Charlesbridge, 2006.

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Murnane, Gerald. Barley patch. Champaign: Dalkey Archive Press, 2011.

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ill, Riopelle Jennifer, ed. Priscilla's patch. Ann Arbor, Mich: Proctor Publications, 1997.

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Sole, Linda. Briar patch. Surrey, England: Severn House, 2011.

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Crawford, Jack. Jack's patch. Birmingham: British Gas, 1991.

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Book chapters on the topic "Patch de la membrane"

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Hilgemann, Donald W. "The Giant Membrane Patch." In Single-Channel Recording, 307–27. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4419-1229-9_13.

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Church, Timothy W., and Matthew G. Gold. "Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method." In Patch Clamp Electrophysiology, 243–57. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_12.

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López-Barneo, José. "Voltage-Dependent Ionic Channels: “Whole-Cell” Recording by Patch-Clamp Techniques." In Cell Membrane Transport, 215–37. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4757-9601-8_12.

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Petersen, Ole. "Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels Via Internal Messengers." In Cell Membrane Transport, 437–50. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4757-9601-8_22.

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Hedrich, R., H. Stoeckel, and K. Takeda. "Electrophysiology of the Plasma Membrane of Higher Plant Cells: New Insights from Patch-Clamp Studies." In The Plant Plasma Membrane, 182–202. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74522-5_8.

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Vassilev, Peter M., and H. Ti Tien. "Reconstitution of Membrane Molecular Mechanisms in Bilayer Lipid Membranes and Patch-Clamp Bilayers." In Subcellular Biochemistry, 97–143. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-9362-7_3.

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Gerhardt, Matthias, Michael Walz, and Carsten Beta. "Fluorescence Readout of a Patch Clamped Membrane by Laser Scanning Microscopy." In Methods in Molecular Biology, 325–39. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3480-5_23.

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Vredenberg, W. J., J. H. A. Dassen, and J. F. H. Snel. "Patch Clamping the Photosynthetic Membrane: A Sensitive Tool to Study Chloroplast Bioenergetics." In Photosynthesis: Mechanisms and Effects, 4271–76. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-3953-3_987.

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Schanne, O. F., M. Le Floch, R. Sauvé, and M. D. Payet. "Membrane Potential of Neonatal Rat Myocytes: A Microelectrode and Patch Clamp Study." In Developments in Cardiovascular Medicine, 156–63. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2621-2_10.

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Wang, Zheng, Dirk L. Ypey, and Rutgeris J. Van den Berg. "Membrane Potential and Action Potential Measurements in Whole Cell and Perforated Patch Configurations." In Signal Transduction — Single Cell Techniques, 187–206. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80368-0_15.

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Conference papers on the topic "Patch de la membrane"

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Harle, Lee, and John Papapolymerou. "3D printed membrane microstrip patch antenna." In 2017 IEEE International Symposium on Antennas and Propagation & USNC/URSI National Radio Science Meeting. IEEE, 2017. http://dx.doi.org/10.1109/apusncursinrsm.2017.8073361.

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Huang, J., and A. Moussessian. "Thin-membrane aperture-coupled L-band patch antenna." In IEEE Antennas and Propagation Society Symposium, 2004. IEEE, 2004. http://dx.doi.org/10.1109/aps.2004.1331853.

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Ayorinde, Emmanuel, Mohammad Al-Zubi, Fouad Mohammad, and Mehmet Akif Dundar. "Investigation of Some Circular Membrane-Patch Acoustic Meta-Materials." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-66720.

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A great need exists for vibration and noise control with thinner and smaller devices, especially at lower frequencies than those at which the usual magnetic and electrical metamaterials operate. Acoustic metamaterials have been under development for such purposes. Some of the present authors have experimentally and numerically investigated circular acoustic metamaterials made of lexan plate and silicon membranes. In this work, the authors extend the investigation to the use of some other materials and relative see if and how well such a construction may be utilized for meta-material applications.
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Baron, Samuel, Benoit Guiffard, and Ala Sharaiha. "Frequency tunable patch antenna with electrically actuated supported polymer membrane." In 2014 44th European Microwave Conference (EuMC). IEEE, 2014. http://dx.doi.org/10.1109/eumc.2014.6986364.

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Jang Ho Kim, Joon-Ho Bae, Jong Hoon Chung, Yun-Hoon Choung, Ki Taek Lim, Seong Jun Choi, Eung Tae Lee, and Ae Lee Im. "Development of Artificial Eardrum Patch to Repair Tympanic Membrane Perforations." In 2008 Providence, Rhode Island, June 29 - July 2, 2008. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2008. http://dx.doi.org/10.13031/2013.24820.

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Hayashida, Yuki, Tomohiro Kinoshita, and Tamami Motomura. "Interfacing neurons through the patch membrane pierced with single-walled carbon nanotubes." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6610547.

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Wilson, Jim R., and Neil A. Duncan. "Modelling the Ion Channel Behaviour of Articular Chondrocytes." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-32661.

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All cells have a membrane potential; this voltage difference arises from the different intracellular and extracellular ion concentrations. In excitable tissue the cell membranes contain ion channels which control the movement of ions and hence control the cell’s membrane potential. Extensive measurements of the electrophysiology of excitable cells has allowed considerable understanding of the ion channels. The Hodgkin-Huxley model [1] was developed from measurements on a squid nerve axon, and it quantifies the changes in membrane conductance due to the opening and closing of specific ion channels. This model has been very successful in describing the electrical behaviour of neurons. Ion channels also exist in non-excitable tissue cells. Patch clamp experiments have demonstrated that ion channels in chondrocytes influence cell’s membrane potential [2]; controls the influx of Ca2+ [3] and may regulate cell proliferation [2]. The objective of this research was to develop a model of ion channel behaviour for connective tissue cells based on the Hodgkin-Huxley model, and to apply this model to reported patch clamp measurements of articular chondrocytes.
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Tamaddoni, Nima, and Andy Sarles. "Characterizing the Sources of Current Generated by a Membrane-Based Hair Cell Sensor." In ASME 2013 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/smasis2013-3141.

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Recently, researchers have developed a method to construct a membrane-based hair cell sensor that generates a measurable current in response to physical disturbance of the hair. Representing the cell membrane, a phospholipid bilayer is formed at the interface of two lipid-encased hydrophilic volumes and a hair is located in a center of one of the volumes act a shaking element. In this work, we study the current generated by free vibration of the hair in a revised hair cell embodiment that uses a hair that is physically supported by the surrounding substrate. The current generated by the sensor is measured by a patch clamp amplifier, and the net charge displaced across the membrane during motion of the hair is computed. Experiments performed with a complete hair cell sensor and various control cases that lack a bilayer indicate that the current measured at 0mV applied across the membrane is due to vibration of the positive electrode that changes the local electromagnetic field. Experiments conducted with both geland liquid-supported membranes indicate that gel-supported membranes have a higher sensitivity of (0.066 pC/mV) than liquid-supported membranes (0.015 pC/mV) as the applied voltage increases. Lastly, the motion of the tip of the hair is imaged using a high-speed camera. This test shows that the hair oscillates at the same frequency observed in the measured current traces, which indicates that transverse bending of the bilayer is the cause for the time rate of change in capacitance in the membrane that produces a voltage-dependent current.
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Thai, Trang T., Gerald R. DeJean, and Manos M. Tentzeris. "Novel ultrasensitive millimeter-wave pressure transducer utilizing a Si membrane and a stacked-patch configuration." In 2009 IEEE Antennas and Propagation Society International Symposium (APSURSI). IEEE, 2009. http://dx.doi.org/10.1109/aps.2009.5171876.

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Nguyen, Mary-Anne, Nima Tamaddoni, and Stephen A. Sarles. "Interrogation of Bilayers in a Multi-Droplet Cluster for Membrane-Based Sensing." In ASME 2015 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/smasis2015-8970.

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The long-term vision of our work is to create a new class of smart material that utilizes networks of active, synthetic cell membranes for sensing, actuation, and energy harvesting. Having multiple membrane structures is specifically targeted because a higher density of functional membranes is expected to enable amplification and collective utility, similar to how living tissues and organisms utilize networks of highly connected cells to accomplish large tasks. While there are several known methods for assembling droplet-based networks of synthetic lipid bilayers, there has been much less effort to develop methods for electrically characterizing each interface in a multi-bilayer-droplet network. This paper specifically focuses on a strategy for using electrical measurements to independently record transmembrane currents occurring at each bilayer in multi-bilayer networks where the number of bilayers present is equal to or greater than the number of droplets in the system. Using a multichannel patch clamp amplifier, we develop a measurement technique for sequentially assigning sensing electrodes to apply a non-zero voltage or function as virtual ground (V=0). Experimental studies on a three-droplet cluster containing three bilayers confirm the validity of the proposed approach for independently interrogating each membrane, and the results allow extension of the method to networks with 4–7 droplets. Furthermore, alamethicin peptide gating is monitored using the measurement cycle in order to interrogate all interfaces. Due to high total membrane area, highly packed systems can provide an increase in the magnitude of sensing current generated by a stimulus. Such amplification could feasibly be employed in droplet-based hair cell sensing applications in which airflow or vibration acts as the perturbation source, and the proposed approach and challenges for interrogating the transduction response in a multi-membrane hair cell sensor are discussed herein.
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Reports on the topic "Patch de la membrane"

1

Hoffman, P., and J. Snell. JSON Merge Patch. RFC Editor, October 2014. http://dx.doi.org/10.17487/rfc7386.

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Hoffman, P., and J. Snell. JSON Merge Patch. RFC Editor, October 2014. http://dx.doi.org/10.17487/rfc7396.

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Dusseault, L., and J. Snell. PATCH Method for HTTP. RFC Editor, March 2010. http://dx.doi.org/10.17487/rfc5789.

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Perry, Anna. Sunrise over Rainbow Patch. Ames: Iowa State University, Digital Repository, November 2016. http://dx.doi.org/10.31274/itaa_proceedings-180814-1627.

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Donaghay, Percy L. Plankton Patch Feasibility Experiments. Fort Belvoir, VA: Defense Technical Information Center, September 1997. http://dx.doi.org/10.21236/ada627832.

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Donaghay, Percy L. Plankton Patch Feasibility Experiments. Fort Belvoir, VA: Defense Technical Information Center, September 2001. http://dx.doi.org/10.21236/ada628051.

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Donaghay, Percy L. Plankton Patch Feasibility Experiments. Fort Belvoir, VA: Defense Technical Information Center, September 1999. http://dx.doi.org/10.21236/ada629834.

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Bierman, A., M. Bjorklund, and K. Watsen. YANG Patch Media Type. RFC Editor, February 2017. http://dx.doi.org/10.17487/rfc8072.

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Donaghay, Percy L. Plankton Patch Feasibility Experiments. Fort Belvoir, VA: Defense Technical Information Center, September 2000. http://dx.doi.org/10.21236/ada609785.

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Hosmer, Chester. Feasibility of Software Patch Verification. Fort Belvoir, VA: Defense Technical Information Center, June 2004. http://dx.doi.org/10.21236/ada425345.

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