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Dissertations / Theses on the topic 'Pathogenic bacteria Identification'

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1

Foo, Chuen-hing, and 符傳興. "Bacteremia due to Elizabethkingia and related species." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208519.

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Elizabethkingia spp. is a gram-negative, non-fermenting rod bacterium that is frequently implicated in hospital outbreaks. Elizabethkingia has a high rate of resistance to antibiotics and a shortage of effective parenteral antibiotics usually occurs in intensive care units. Infection includes neonatal sepsis and meningitis. Recently, a new species of Elizabethkingia, which is closely related to E. meningoseptica ATCC 13253 and E. miricola GTC862, was reported as a human pathogen in Central Africa and named E. anophelis. Our investigation involved 27 Elizabethkingia clinical isolates, which wer
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2

O'Hara, Heather Marie. "Comparison of the different spectra of some selected bacteria." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/27161.

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3

Kondacs, Laszlo. "Novel substrates for the improved detection and identification of pathogenic bacteria." Thesis, University of Sunderland, 2018. http://sure.sunderland.ac.uk/10222/.

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Many diseases are caused by pathogenic bacteria. A key example of this is sepsis, which is mostly caused by staphylococci and Gram-negative bacteria. In addition, the highly resistant ESKAPE pathogens are responsible for the majority of hospital acquired infections. In order to treat bacterial infections effectively, and to avoid promoting bacterial resistance against antibacterial drugs, the correct agents must be used, for which in turn the detection and identification of pathogenic strains is essential. This research aims to develop selective chromogenic culture media, by introducing new an
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4

Li, Kwan-hing. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31971982.

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5

Li, Kwan-hing, and 李群卿. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31971982.

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6

Yeung, Shiu-yan, and 楊兆恩. "Update and evaluation of 16SpathDB, an automated comprehensive database for identification of medically important bacteria by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193552.

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Identification of pathogens is one of the important duties of clinical microbiology laboratory. Traditionally, phenotypic tests are used to identify the bacteria. However, due to some limitations of the phenotypic tests, the bacteria may not be identified sometimes and cannot be identified promptly. 16S rRNA gene sequencing is a rapid and accurate method to achieve this target. It is especially useful for identify rare or slow growing bacteria. However, the interpretation of the 16S rRNA gene sequencing result is one of the challenging duties to laboratory technicians and microbiologists. Apar
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7

Male, Abigail. "Identification of inhibitors of protein-protein interactions essential for virulence in pathogenic bacteria." Thesis, University of Southampton, 2014. https://eprints.soton.ac.uk/369351/.

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There is a continous requirement for broad-spectrum post-exposure antibiotic therapeutics. Meeting this challenge relies on the production of compounds that successfully disrupt bacterial systems identified as both conserved and essential. Here, inhibitors of protein-protein interactions involved in the Phage shock protein response and toxin internalisation, within Burkholderia pseudomallei and Bacillus anthracis, respectively have been identified. This was achieved using a high-throughput screen that combines a bacterial reverse two-hybrid system and an intein-mediated method for the generati
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8

Kaittanis, Charalambos. "Magnetic nanosensors for multiplexed bacterial pathogenesis identification." Doctoral diss., University of Central Florida, 2010. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/4610.

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Developing diagnostic modalities that utilize nanomaterials and miniaturized detectors can have an impact in point-of-care diagnostics. Diagnostic systems that (i) are sensitive, robust, and portable, (ii) allow detection in clinical samples, (iii) require minimal sample preparation yielding results quickly, and (iv) can simultaneously quantify multiple targets, would have a great potential in biomedical research and public healthcare. Bacterial infections still cause pathogenesis throughout the world (Chapter I). The emergence of multi-drug resistant strains, the potential appearance of bacte
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9

Pierce, Carrie. "High throughput mass spectrometry for microbial identification." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/43741.

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Bacteria cause significant morbidity and mortality throughout the world, including deadly diseases such as tuberculosis, meningitis, cholera, and pneumonia. Timely and accurate bacterial identification is critical in areas such as clinical diagnostics, environmental monitoring, food safety, water and air quality assessment, and identification of biological threat agents. At present, there is an established need for high throughput, sensitive, selective, and rapid methods for the detection of pathogenic bacteria, as existing methods, while nominally effective, have failed to sufficiently redu
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10

Gamieldien, Junaid. "Novel genomic approaches for the identification of virulence genes and drug targets in pathogenic bacteria." Thesis, University of the Western Cape, 2001. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4400_1185438906.

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<p>While the many completely sequenced genomes of bacterial pathogens contain all the determinants of the host-pathogen interaction, and also every possible drug target and recombinant vaccine candidate, computational tools for selecting suitable candidates for further experimental analyses are limited to date. The overall objective of my PhD project was to attempt to design reusable systems that employ the two most important features of bacterial evolution, horizontal gene transfer and adaptive mutation, for the identification of potentially novel virulence-associated factors and possible dru
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11

Boucher, David. "Identification and characterisation of in vivo expressed genes of Pasteurella multocida." Monash University, Dept. of Microbiology, 2004. http://arrow.monash.edu.au/hdl/1959.1/9657.

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12

Pollard, Stephanie Kay. "Identification of food safety risks at Virginia farmers' markets and development of a food safety plan to help farmers market managers." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/78196.

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The growing popularity of farmers' markets coupled with a high percentage of produce-related foodborne outbreaks highlights the need for an emphasis on food safety within these markets to protect farmers, patrons and local economies. The number of farmers' markets registered in the United States has almost tripled in the last 15 years. Fresh produce constitutes the majority of food sold at farmers'markets. Between 1998 and 2008, raw produce accounted for almost half of the 4,589 foodborne illness outbreaks linked to a specific commodity. This research was conducted to identify practices at
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13

Nejad, Pajand. "Pathogenic and ice-nucleation active (INA) bacteria causing dieback of willows in short rotation forestry /." Uppsala : Dept. of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/200524.pdf.

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14

Woo, Chiu-yat Patrick, and 胡釗逸. "Defining novel clinical syndromes and emerging pathogens." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B27497343.

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15

Beshr, Ghamdan [Verfasser], and Alexander [Akademischer Betreuer] Titz. "Biochemical characterization and ligand identification of Pseudomonas aeruginosa lectins and their orthologs in other pathogenic bacteria / Ghamdan Beshr ; Betreuer: Alexander Titz." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2018. http://d-nb.info/1167770781/34.

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16

Golanowska, Malgorzata. "Characterization of Dickeya solani strains and identification of bacterial and plant signals involved in induction of virulence." Thesis, Lyon, INSA, 2015. http://www.theses.fr/2015ISAL0087/document.

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Les bactéries pectinolytiques des genres Pectobacterium (ancien nom Erwinia carotovora) et Dickeya (ancien nom Erwinia chrysanthemi) sont les agents des maladies de la jambe noire et de la pourriture molle. Ils provoquent des dommages aux cultures et des pertes économiques élevées. Les pertes causées par les bactéries pectinolytiques sont évaluées à environ 2 à 10% du rendement de pommes de terre, en fonction de l'année. En 2009, les pertes en pommes de terre en Europe ont été estimées à 250 millions d'euros. Au cours des dernières années, des souches de Dickeya ont été de plus en plus souvent
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17

Panji, Sumir. "Identification of bacterial pathogenic gene classes subject to diversifying selection." Thesis, University of the Western Cape, 2009. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_5842_1297942831.

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<p>Availability of genome sequences for numerous bacterial species comprising of different bacterial strains allows elucidation of species and strain specific adaptations that facilitate their survival in widely fluctuating micro-environments and enhance their pathogenic potential. Different bacterial species use different strategies in their pathogenesis and the pathogenic potential of a bacterial species is dependent on its genomic complement of virulence factors. A bacterial virulence factor, within the context of this study, is defined as any endogenous protein product encoded by a gene th
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18

Tam, Man-wah. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/hkuto/record/B31970783.

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19

譚文華 and Man-wah Tam. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31970783.

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20

Chambers, Jacob Richard. "Identification and Characterization of the Hfq protein and small RNAs in Francisella novicida." OpenSIUC, 2011. https://opensiuc.lib.siu.edu/dissertations/370.

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Francisella tularensis is the causative agent of the disease tularemia and a potential bioterrorism agent. Few regulators have been identified in this organism and little is known about its genetic regulatory networks. In this dissertation project, culture-based and molecular methods were used to both determine the role of the RNA chaperone protein Hfq and identify potential novel small RNAs in F. tularensis subsp. novicida strain U112. The Hfq protein is recognized as an important regulatory factor in a variety of cellular processes, including stress resistance and pathogenesis, and has been
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21

Wiltshire, Michael David. "The identification of genes important to the growth of Staphylococcus aureus in in vitro models mimicking infection." Thesis, University of Sheffield, 2001. http://etheses.whiterose.ac.uk/14812/.

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Staphylococcus aureus is a major pathogen, which causes a wide range of infections. Despite its obvious clinical importance, little is known about the mechanisms of pathogenesis. An in vitro model mimicking infection was developed in order to identify putative virulence determinants. The model involves the growth of S. aureus in serum under microaerobic conditions. All known virulence factors tested were shown not to be required for growth, or preferentially expressed, in serum. Tn917 transposon libraries of S. aureus were screened to identify genes preferentially expressed in serum, compared
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22

Zhang, Xueying. "Identification, properties, and application of enterocins produced by enterococcal isolates from foods." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1206069582.

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23

Leung, Ka-ming, and 梁家銘. "Isolation, identification and establishment of bacterial culture collection of fish pathogens in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/207649.

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The importance of fish culture has been increasing since 1990’s. The steady growth of fish culture helps to ensure a stable supply of fish for human consumption. However, when compared with capture fisheries, production from fish culture is greatly influenced by fish diseases. Outbreaks of fish diseases have caused great economic loss to fish culture. Research has been conducted to understand and reduce the occurrence of fish diseases in fish culture. In Hong Kong, bacterial infection is the most common cause of fish diseases. This project is therefore directed to isolate and identify the caus
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24

Wright, Lynda J. "Identification and characterisation of components expressed by gram-positive bacterial pathogens during human infection." Thesis, University of Sheffield, 2008. http://etheses.whiterose.ac.uk/10312/.

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Gram-positive pathogens are responsible for a wide range of global diseases, including nosocomial infections. The increasing incidence of antibiotic-resistant strains warrants the development of novel therapeutic strategies to combat these organisms.
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25

Lau, Ken Wan Keung. "The identification of novel marine bacteria, and the construction of single chain fragment variable antibodies for the control of a viral pathogen /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?AMCE%202006%20LAU.

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26

Richter, Lisa [Verfasser]. "Identification of immunological properties of natural products for the treatment of resistant tumors and bacterial pathogens / Lisa Richter." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2020. http://d-nb.info/1202603734/34.

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27

Zhou, Binbin. "Identification and characterization of target genes of RRS1-R, a protein conferring resistance in Arabidopsis thaliana to the pathogenic bacterium Ralstonia solanacearum." Toulouse 3, 2014. http://thesesups.ups-tlse.fr/2604/.

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Ralstonia solanacearum, agent du flétrissement bactérien, affecte près de 200 espèces végétales. Les gènes RRS1-R confèrent à l'écotype d'A. Thaliana Nd-1 une résistance à différentes souches de R. Solanacearum. RRS1-R code une protéine de structure modulaire associant les domaines typiques de nombreuses protéines de résistance TIR-NBS-LRR et un domaine signature de facteurs de transcription WRKY. Dans l'écotype sensible Col-0, le gène RRS1-S code pour une protéine qui présente une structure très semblable. Au cours de ce travail, nous avons montré que les gènes RRS1-R et RRS1-S s'expriment es
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28

Al_griw, Huda Hm. "Molecular detection of bloodstream pathogens in critical illness." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/molecular-detection-of-bloodstream-pathogens-in-critical-illness(5f143a31-3694-454c-8940-5ae434f1eb31).html.

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Background: Critically ill patients are at particular risk of developing bloodstream infection. Such infections are associated with the development of sepsis, leading to a marked increase in mortality rate. Early detection of the causative organism and appropriate antibiotic treatment are therefore critical for optimum outcome of patients with nosocomial infection. Current infection diagnosis is based on standard blood culture techniques. However, microbiological culture has a number of limitations, not least that it takes several days to confirm infection and is therefore not useful in direct
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29

Rezende, de Castro Moretti Fernanda. "Identification of candidate resistance metabolites to Leifsonia xyli subsp. xyli in sugarcane through metabolomic profiling." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1512478087354334.

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30

Tallent, Sandra McKenzie. "Identification and Characterization of Helper Phage Gene Products Involved in Mobilization of Staphylococcal Pathogenicity Island SaPI1." VCU Scholars Compass, 2007. http://scholarscompass.vcu.edu/etd/1223.

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Staphylococcal pathogenicity island SaPI1 is excised from genomic DNA and extrachromosomal copies are amplified during the vegetative growth of staphylococcal phage 80α. The amplified genetic element is subsequently encapsidated and transduced at very high frequency. Previous studies have demonstrated that the transducing particles have virions with tails that appear identical to those of helper phage 80α but have smaller capsids, commensurate with the smaller genome of the SaPI (Lindsay et.al., 1998). The morphology of the transducing particles, coupled with the observation that the genomic s
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31

Appel, Maryke. "Cloning and identification of genes involved in the interaction between the bacterial stone fruit pathogen Pseudomonas syringae pv. syringae strain NV and plum trees." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52165.

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Thesis (PhD)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: Bacterial canker of stone fruit, caused by Pseudomonas syringae pv. syringae, is one of the most destructive crop diseases in South Africa. Chemical control has failed completely and effective long-term management strategies will have to rely on the breeding of resistant host trees. To assist in such breeding programmes, investigations into the molecular basis of the interaction between P. s. pv. syringae and stone fruit trees have been undertaken in collaboration with the ARC-Fruit, Wine and Vine Research Institute in St
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32

Cossé, Mathilde. "Identification et caractérisation d'un nouvel effecteur précoce de Chlamydia trachomatis." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066083/document.

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C. trachomatis est une bactérie Gram-négative intracellulaire obligatoire et un pathogène humain. Première cause de maladie sexuellement transmissible d'origine bactérienne, elle est également responsable, dans les pays en développement, d'infections oculaires pouvant conduire à la cécité (trachome). Son cycle de développement bi-phasique a lieu au sein d'un compartiment appelé inclusion. Grâce à un système de sécrétion de type 3 (SST3), Chlamydia sécrète des protéines dans le cytosol de la cellule afin de promouvoir sa survie et sa multiplication. Ces protéines sont désignées sous le terme d'
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33

Kühbacher, Andreas. "Identification of new molecular effectors involved in the internalization of the bacterial pathogen Listeria monocytogenes in host cells using high-throughput RNAi screening and targeted approaches." Paris 7, 2013. http://www.theses.fr/2013PA077193.

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La bactérie pathogène Listeria monocytogenes infecte les cellules non-phagocytaires, s'échappe de la vacuole et se réplique dans le cytoplasme de la cellule-hôte. L'internalisation implique l'interaction de récepteurs de la cellule avec des protéines de surface de la bactérie de la famille des internalines. Notamment, InlA interagit avec E-cadherin et In1B interagit avec le récepteur de facteur de croissance hépatocytaire Met, ce qui provoque l'activation de la cascade de signalisation, qui déclenche l'internalisation de la bactérie, dépendant de l'actine et de la clathrine. Afin de mieux comp
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34

Goad, David Wesley. "Methods for detection and identification of pathogenic bacteria." 2008. http://digital.library.okstate.edu/etd/umi-okstate-2826.pdf.

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35

Zaraee, Negin. "Interferometric imaging for pathogenic bacteria identification and antibiotic susceptibility testing." Thesis, 2021. https://hdl.handle.net/2144/42614.

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Pathogenic bacterial infections are a serious threat to public health, claiming millions of lives every year. In order to contain the spread of infectious diseases sensitive and timely diagnosis of pathogenic bacteria is of significant importance. The rapid detection of low abundance analytes is still challenging in the most common bacteria detection techniques including, culture and colony counting, Enzyme-linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR). Conventional bacteria detection techniques suffer from limitations such as low sensitivity, cost, long procedural tim
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36

Hsieh, Pei-Chi, and 謝沛旂. "Identification of pathogenic bacteria in body fluids of canine and feline by 16S rRNA." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/us58bv.

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碩士<br>國立臺灣大學<br>臨床動物醫學研究所<br>105<br>Regarding to urinary tract and bloodstream infections in dogs and cats, bacterial culture is the gold standard to confirm the diagnosis. As the duration of culture and strain identification need 72 hours at least, the clinicians often use antibiotics before the cultural results; consequently the problems of antibiotic abuse and resistance increase. Thus the less time and higher sensitivity diagnostic methods were indeed needed. The 16S rRNA gene is used for phylogenetic studies as it is highly conserved between different species of bacteria. In addition to h
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Lall, Namrita. "Isolation and identification of Naphthoquinones from Euclea natalensis with activity against Mycobacterium tubercolosis, other pathogenic bacteria and Herpes simplex virus." Thesis, 2001. http://hdl.handle.net/2263/25097.

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The antimycobacterial activity of twenty South African medicinal plants were investigated using two methods commonly used; the conventional agar plate method and the BACTEC radiometric method. Fourteen of the twenty acetone extracts of medicinal plants used to treat pulmonary diseases showed inhibitory activity at a concentration of 0.5 mg/ml against a sensitive strain of Mycobacterium tuberculosis using the conventional agar plate method. These fourteen extracts were also tested against M tuberculosis by the BACTEC radiometric method against a sensitive as well as a strain resistant to the dr
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HsiYang and 楊晞. "Identification and characterization of mobilizable plasmids that benefit bacterial pathogens during infections." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/853445.

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39

Almeida, Maria Teresa da Conceição Malheiro Pinto de. "Identification of new host signaling pathways hijacked by bacterial pathogens during infection." Tese, 2015. https://repositorio-aberto.up.pt/handle/10216/79924.

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Almeida, Maria Teresa da Conceição Malheiro Pinto de. "Identification of new host signaling pathways hijacked by bacterial pathogens during infection." Doctoral thesis, 2015. https://repositorio-aberto.up.pt/handle/10216/79924.

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41

Yang, Wen-Shin, and 楊雯馨. "Identification and detection of bacterial pathogens infecting papaya in central and southern Taiwan." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/63216427490919062936.

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碩士<br>中興大學<br>植物病理學系所<br>95<br>A bacterial disease with symptoms as that of black rot on papaya, seriously occurred in central and southern Taiwan in recent years. Based on physiological, biochemical and pathogenicity tests, there were 36 strains of plant pathogenic bacteria isolated from diseased papayas from Pingtung and Changhua, including 21 strains of Erwinia sp., 5 strains of Pectobacterium carotovorum subsp. carotovorum and 10 strains of Pseudomonas aeruginosa. Physiological and biochemical assays revealed that the characteristics of the Erwinia sp. strains were similar to that of Pect
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Chu, Chun-Yu, and 朱俊育. "Development of an Oligonucleotide Array for Identification of Bacterial Pathogens of Bovine Mastitis." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/57444483394659710774.

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碩士<br>國立屏東科技大學<br>食品科學系所<br>98<br>Cow mastitis is caused by a wide variety of microbial pathogens. In this study, an oligonucleotide array was developed to identify the pathogens causing mastitis. These pathogens include Corynebacterium bovis, Enterococcus faecalis, Escherichia coli, Listeria monocytogenes, Mycoplasma bovis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hyicus, Staphylococcus intermedius, Staphylococcus simulans, Staphylococcus xylosus, Streptococcus agalactiae, Streptococcus d
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Lin, Jiunn-Yow, and 林俊佑. "Rapid Identification of Bacterial Food Poisoning Pathogens by High-Resolution Melting Curve Analysis." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/24203145926023478068.

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碩士<br>輔仁大學<br>生命科學系碩士班<br>101<br>Foodborne illness is one of the most common infectious diseases worldwide. Among them, food poisoning syndromes caused by bacterial pathogens are known to be very diversified and complicated. Therefore, how to clarify food poisoning pathogens as soon as possible is an important issue. The conventional methods used for the diagnosis of bacterial food poisoning pathogens are very time-consuming. However, with the advance of cross-cutting diagnostic technology, many new tools are available for an accurate and rapid bacterial detection and diagnosis. Among them, nu
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Πρωτόπαπα, Μαρία. "Μοριακή ανίχνευση παθογόνων βακτηρίων που ενέχονται στην περιοδοντίτιδα". Thesis, 2010. http://nemertes.lis.upatras.gr/jspui/handle/10889/3683.

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Τα Gram αρνητικά βακτήρια είναι οι κύριοι αιτιολογικοί παράγοντες της περιοδοντικής νόσου, όπως τα βακτήρια Porphyromonas gingivalis, Actinοbacillus actinomycetemcomitans και Bacteroides forsythus. Σκοπός της εργασίας είναι η μοριακή ανίχνευση και ταυτοποίηση των βακτηρίων αυτών, σε δείγματα ασθενών με περιοδοντίτιδα, μέσω εντοπισμού ειδικών αλληλουχιών του DNA του γονότυπου κάθε παθογόνου. Τα δείγματα συλλέγονται σε PBS και DNA παρασκευάζεται με το QIAmp Tissue kit (Qiagen). Η μοριακή ανάλυση γίνεται με PCR και multiplex PCR, με ένα εκκινητή ειδικό για κάθε βακτήριο και ένα κοινό για τα τρί
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Wen, Bing-Cheng, and 溫秉丞. "Detection and identification of the pathogen of bacterial meningitis by Surface Enhanced Raman Scattering (SERS)." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/69350776873346999616.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>98<br>Before the discovery of antibiotic, meningitis was a fatal disease. At the beginning of the infection, the symptoms of meningitis are hard to differentiate from other illnesses. Besides, it takes about 2~3 days to culture and diagnosis the pathogen after acquisition of the Cerebrospinal fluid(CSF) from patient. It is fatal for the patients if appropriate antibiotics are not prescribes soon after infection, therefore, it is of utmost important to identify the pathogen as quickly as possible. Raman Scattering was discovered in 1928, it has been used to analyze
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Chen, Yi-Jeng, and 陳以錚. "Bacterial wilt of vegetable sweet potato in Taiwan - pathogen identification, inoculum sources and management strategy." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/60053076402405567788.

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博士<br>國立中興大學<br>植物病理學系所<br>103<br>Abstract Sweet potato [Ipomoea batatas L. (Lam)], the dicotyledonous plant of the family Convolvulaceae, is the seventh most important food crop in the world after wheat, rice, maize, potato, barley and cassava. Besides food supply, sweet potato has diverse uses in green source, ornamental, feed, starch and liquor manufacture, human consumption, biofuel and bioplastic production, etc. Many cultivars have been currently developed for different uses through the world. Among these cultivars, vegetable sweet potato (VSP) has been bred for edible leafy vegetable a
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Hwang, Chung-Hsing, and 黃中興. "Rapid Detection and Identification of Foodd-Borne Bacterial Pathogens by Multiplex PCR and Restriction Endonuclease Digestion." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/16304518326196407602.

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碩士<br>國立中山大學<br>生物科學系研究所<br>89<br>英文摘要 Multiplex PCR amplification of 16S rRNA gene、virA、tpl、and H1d genes was developed enabling simultaneous detection in Escherichia coli,an indicator of fecal contamination and food-borne microbial pathogens,Shigella flexneri、Citrobacter freundii、Salmonella typhi、Vibrio cholerae、Vibrio parahaemolyticus、and Staphylococcus aureus。Each of the nine pairs of oligonucleotide primers was found to support PCR amplifications of only its targeted gene。The optimized multiplex PCR reaction utilized a primer annealing temperature of 59 ℃and used agarose gel electroph
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