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Dissertations / Theses on the topic 'Pathology, Division of Chemical Pathology'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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1

Delport, Darnielle. "The development and application of a polymerase chain reaction (PCR) based assay to determine the impact of genetic variation in South African patients diagnosed with depression." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86564.

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Thesis (MPath)--Stellenbosch University, 2014.<br>ENGLISH ABSTRACT: Major Depressive Disorder (MDD) is a severe debilitating medical condition that may lead to suicide. Due to a poor understanding of the biological mechanisms underlying the disease process therapeutic decisions are usually taken using a ‘trial and error’ approach. This is not ideal since many treatments do not work as expected for all individuals. Studies have shown that only half of MDD patients receive the appropriate treatment, whereas many patients have adverse response to anti-depressants. These may include weight gain an

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2

Patel, Soyab Ahmed. "Chemical imaging for clinical pathology and bioanalysis." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.488821.

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3

Pawson, Adam James. "A mutational analysis of the roles of cytoplasmic domains of the gonadotropin-releasing hormone receptor in coupling and internalization." Doctoral thesis, University of Cape Town, 1999. http://hdl.handle.net/11427/26779.

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The G protein-coupled receptor (GPCR) family is the largest group of homologous proteins in the human genome. GPCRs are of prime physiological and medical importance as the actions of a wide range of hormones and drugs are mediated by these receptors. The gonadotropin-releasing hormone (GnRH) receptor is a member of the GPCR family, and plays a central role in the reproductive system. GnRH analogues are used therapeutically in a number of human disorders. All GPCRs contain 7 largely α-helical transmembrane domains. An arginine residue located at the cytosolic boundary of the third transmembran

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4

Rapulana, Antony Morwamoche. "Dried spot cards to analyse biologic fluids for diagnostic investigation of patients." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29858.

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Background: Collection of biologic fluid for laboratory analysis requires relatively large samples, often with additives, and transport in fragile tubes. The analytes or matrices may be unstable so testing needs to be carried out quickly. Collection of these biologic fluids and drying them on filter paper can lower the cost of transporting the sample to the laboratory, avoid instability of the matrix, and degradation of the analytes. Aim: The aim of this project was to develop an inexpensive, convenient, comprehensive and reproducible patient sample collection system which ensures integrity

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5

De, Wet Heidi. "The nucleotide binding domains of multidrug resistance-p-glycoproteins." Doctoral thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/2690.

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6

Cunningham, Jessica. "A molecular perspective on the family Testudinidae batsch, 1788." Doctoral thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/2689.

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Bibliography: leaves 228-252.<br>The Family Testudinidae have a diverse distribution and, although limited to tropical and subtropical latitudes, are present on all continents with the exception of Australia and Antartica. Their evolutionary history dates back to the late Paleogene at least, and periods of diversification and expansion appear to be closely associated with global climate change, particularly at the border of the Oligocen-Miocene transition.

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7

Marakalala, Mohlopheni Jackson. "Inhibition of a Mycothiol biosynthetic enzyme and a detoxification enzyme as anti-tubercular drug targets." Doctoral thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/2694.

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8

Troskie, Brigitte Elise. "Cloning and characterisation of gonadotropin-releasing hormone receptors from species in non-mammalian vertebrate classes : amphibia and osteichthyes." Doctoral thesis, University of Cape Town, 1998. http://hdl.handle.net/11427/26916.

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Two or more forms of gonadotropin-releasing hormone (GnRH) have been isolated from most vertebrate species. In most species, GnRH variants have been shown to occur in distinct areas of the peripheral and central nervous systems, the gonads and other peripheral organs. Although GnRH is a primary regulator of gonadotropin secretion, it has been shown to have additional roles such as the regulation of growth hormone secretion in goldfish and the inhibition of a potassium current (M-current) in amphibian sympathetic ganglia. This raises the possibility of the occurrence of multiple GnRH receptor s

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9

Ott, Thomas Ruthard. "Receptor activation in GNRH receptors." Doctoral thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/2700.

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10

Mbewe, Boniface. "Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)." Doctoral thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/2696.

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Includes bibliographical references.<br>The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of contaminating proteins by anion exchange chromatography (HGXPRT does not bind under the conditions used), followed by Reactive Red 120 agarose affinity chromatography.

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11

Phehane, Vuyisile Ntosi. "The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT)." Doctoral thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/2701.

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Bibliography: leaves 231-243.<br>We have expressed and purified human, two forms of P. falciparum, and Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HPRT) in E. coli using the pET expression system. The cDNA encoding the ORF of HPRT was amplified by PCR and transformed into E. coli cells using standard methods. Expression was induced by IPTG and reached about 13% of the total cell protein for all four proteins. The HPRTs were purified by nickel affinity chromatography most of the expressed protein could be isolated from the crude supernatant fraction in a soluble form. Huma

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12

Seekoe, Tshepo W. "Mechanism of sarcoplasmic reticulum Ca-ATPase and multidrug resistance P-glycoprotein probed by Pi-HOH oxygen exchange." Doctoral thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/2702.

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13

Ismail, Wan Iryani Wan. "Molecular Basis of insulin resistance induced by antiretroviral drugs." Doctoral thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/4398.

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14

Swarts, Liezel Catharine. "DNA analysis of Ornithine Transcarbamylase (OTC) deficiency in South African patients." Master's thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/26752.

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Hyperammonaemia is not an infrequent presentation in the newborn or neonatal period. While the majority are transitory in nature and due to infective processes or liver pathology/immaturity, a significant number are due to defects in enzymes of the urea cycle. This cycle has evolved to cope with waste nitrogen disposal and the de novo synthesis of arginine. There are five distinct enzymatic steps in the urea cycle, and defects in each, result in a biochemically distinct disease. Four of these diseases, deficiencies of carbamyl phosphate synthetase (CPS), ornithine transcarbamylase (OTC), argin

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15

Ndlovu, Mbali. "Validation of an adjusted calcium formula using the Roche calcium (NMBATPA) and albumin (BCG) methods at Groote Schuur Hospital." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/25381.

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ABSTRACT: Validation of an adjusted calcium formula using the Roche calcium (NMBATPA) and albumin (BCG) methods at Groote Schuur Hospital Introduction: Most laboratories continue to adjust serum total calcium (tCa) concentration for serum albumin as a surrogate marker of calcium status, despite the availability of ionised calcium (iCa) measurement. Current recommendations by the Association for Clinical Biochemistry and Laboratory Medicine (ACB) advocate that laboratories should use formulae specific for their tCa and albumin methods and analytical platforms. The National Health Laboratory Ser

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16

Stanfliet, John Christian. "Evaluation of serum prolidase activity as a marker for liver fibrosis in suspected liver disease." Master's thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/10805.

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Includes bibliographical references (leaves 67-72).<br>Liver dysfunction is common, often unrecognised and likely to increase in incidence in the population in parallel with the obesity and attendant type 2 diabetes mellitus epidemics. Liver fibrosis is a significant finding in liver pathology as it imparts important clinical staging and prognostic information, is a risk marker of adverse clinical outcome yet, even if advanced, is capable of reversal. Histological examination of liver biopsy material is the reference standard in the assessment of liver fibrosis, but it is impractical to biopsy

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17

De, Wet Barend J. M. "Investigation of cystathionine β-synthase as a cause of mild hyperhomocysteinaemia in patients with peripheral vascular disease". Master's thesis, University of Cape Town, 1999. http://hdl.handle.net/11427/25800.

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Hyperhomocysteinaemia is a recently established risk factor for the development of vascular disease and is caused by a variety of defects in the metabolism of methionine as well as dietary deficiencies of the vitamin cofactors (B6, B12 and folate) of the enzymes involved in methionine metabolism. Cystathionine β-synthase (CBS) is the most common genetic cause of homocystinuria, the severe form of the disease. The incidence of CBS deficiency in a group of 12 young patients of varied ethnic origin, who had peripheral vascular disease (PVD) that could not be ascribed to any of the conventional ri

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18

Seekoe, Tshepo W. "Characterisation of the reaction of 1,4-phenylenebismaleimide with Ca²⁺-ATPase and elucidation of the intramolecular crosslink site." Master's thesis, University of Cape Town, 1997. http://hdl.handle.net/11427/26972.

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The SR Ca²⁺-ATPase is an ATP driven pump that removes calcium from the sarcoplasm and myofibrils to allow muscle relaxation. The sulfhydryl crosslinker, 1,4- phenylenebismaleimide, reacts with Ca²⁺-ATPase (110 kD) to form a species with an apparent molecular weight of 125 kD, as well as dimers and high order oligomers, on SDS-P AGE. During the course of this study we have optimised and characterised the reaction of 1,4-phenylenebismaleimide with SR Ca²⁺-ATPase to produce the 125 kD species that is reminiscent of an E 125 species formed by intramolecular crosslink with glutaraldehyde. The gluta

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19

Omar, Feirdoz. "Investigation of High Molecular weight adiponectin in HIV -Infected patients on antiretroviral therapy." Master's thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/2699.

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This project aims to investigate the role of multimeric (high molecular weight) adiponectin in the development of metabolic disease resulting from anti-retroviral therapy. Specifically, the aim is to quantify the circulating levels of both total and high molecular weight (HMW) adiponectin and to establish whether a link exists between HMW adiponectin levels and susceptibility to HIV-induced lipodystrophy. Although total adiponectin levels have been shown to be significantly reduced in patients with HIVinduced lipodystrophy, there is no information on whether HMW adiponectin, which appears to b

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20

van, der Watt George Frederick. "Whole Blood Mitochondrial DNA Depletion in Human Immunodeficiency Virus-Infected Children." Master's thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/2705.

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Background: Nucleoside reverse transcriptase inhibitors (NRTIs) interfere with mitochondrial DNA polymerase gamma causing significant toxic effects, including fatal lactic acidosis. Little is known about mitochondrial DNA (mtDNA) in human immunodeficiency virus (HIV) infected children who face a lifetime exposure to these agents. We performed a cross sectional observation of mtDNA levels in whole blood in a pediatric population to ascertain the relationship between mtDNA, NRTI regimens and parameters of HIV-infection severity. Methods: Whole blood mt:nDNA ratios were determined by real-time PC

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21

Haarburger, David Richard. "Serum Proteome Changes Following HIV Infection." Master's thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/2691.

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22

Henshall-Howard, Marie-Paule Alix Emilie. "An investigation of the role of N102 of the GnRH receptor in determining the potency of C-terminally modified agonist analogs." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/2692.

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Bibliography : leaves 84-104.<br>Three GnRH receptors with the mutations N102A, N102D and N102K, which were derived from the human GnRH receptor by site directed mutagenesis, were expressed in COS-1 cells. The effects of these mutations on the potency of seven C-terminally modified GnRH agonist analogs for the stimulation of IP production were determined.

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23

Hutchinson, Emerentia. "The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction." Master's thesis, University of Cape Town, 1998. http://hdl.handle.net/11427/26968.

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Gonadotropin-releasing hormone (GnRH), a central regulator of reproductive function in all vertebrates, exerts its effects via binding to the GnRH receptor (GnRHR) in the pituitary gonadotrophs. The GnRHR is a member of the G-protein coupled receptor (GPCR) superfarnily. A second form of the GnRHR (type II), other than the pituitary gonadotrope GnRHR (type I) has been proposed to exist and to play a role other than the classical endocrine role of the pituitary GnRHR. Elucidation of amino acid residues of the GnRHR that are crucial for ligand binding, activation of the receptor, and coupling to

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24

Coutts, Natalie June. "Comparative molecular genetics of the German Shepherd dog." Master's thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/8622.

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Includes bibliographical references (leaves 105-111).<br>Microsatellite markers were used to measure genetic diversity and population differentiation within and between domestic dog breeds. The German Shepherd Dog was compared with typical outbred mongrel dogs, Dachshunds, Staffordshire Bull Terriers and a cohort of other pedigreed dogs representing 30 recognised breeds. Although archaeological records report that grey wolves (Canis lupus) were domesticated approximately 14 000 years ago, mtDNA analysis suggests that domestic dogs (Canis familiaris) and grey wolves diverged in multiple events

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25

Benjamin, Ryan. "Design and prototype of an external quality assurance program for urine bicarbonate." Master's thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/10080.

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This dissertation validates a Beckman-Coulter DxC(R) assay for total bicarbonate in urine and then proceeds to design, prototype and cost an inter-laboratory comparison (ILC) program for the above urine bicarbonate based on the validation. Furthermore, this work serves as a case study for how to establish proficiency testing - and thereby achieve accreditation - for tests without external quality assurance because of analyte instability.

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26

Kelso, Janet. "Quantification of genetic variation on Island-breeding populations of Procellariiformes : an assessment of the impact of the longline fishing industry on seabirds." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/2693.

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Bibliography: p. 83-94.<br>The number of albatrosses that are killed on longlines in the Southern ocean is conservatively estimated to be 44 000 birds per annum. These numbers are biologically significant since albatrosses are a prime example of an extreme K-selected species. Ongoing long line fishing in the Southern ocean could lead to a decrease in the size of breeding colonies, and is a cause for major concern as it may impact the long-term survival of these birds. Quantifying genetic variation in threatened populations is a valuable application of molecular biology in conservation. In this

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27

Olufadi, Rasaq. "Screening for thiopurine s-methyltransferase (TPMT) gene mutations in South Africa." Master's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/2698.

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Includes bibliographical references.<br>Several studies have shown that patients with low TPMT activity are at risk for severe and potentially fatal haematopoietic toxicity when treated with conventional doses of thiopurine drugs. Genetic polymorphism in the TPMT gene is an important determinant of mercaptopurine toxicity. Patients with mutations in the TPMT gene have a less efficient methylation process, and are therefore, predisposed to severe myelosuppression.

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28

Varhol, Richard Joseph. "The molecular systematics of Southern African Testudinidae." Master's thesis, University of Cape Town, 1998. http://hdl.handle.net/11427/26918.

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Sixteen of the world's 42 species of land tortoises occur in Africa, 10 of which are endemic to southern Africa. South Africa itself, which occupies 0.8% of the earth's total land mass, has the highest tortoise biodiversity in the world, with 13 species. This is the first study to use molecular techniques to investigate the evolutionary history of this group, which displays an unusually high level of speciation on the continent. Four hundred and fifty base pairs of mtDNA cytochrome b sequence were obtained, using direct PCR-based sequencing, from 32 individual tortoise blood samples, comprisin

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29

Matshikiza, Maia Thandi. "Antioxidant roles of uric acid and tyrosine in mammalian erythrocytes." Master's thesis, University of Cape Town, 2003. http://hdl.handle.net/11427/2695.

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30

Rusch, Jody Alan. "Using urinary MCP-1 and TWEAK to assess disease activity in a cohort of South African patients with lupus nephritis." Master's thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33899.

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Background: Renal involvement is common in systemic lupus erythematosus (SLE) and can lead to chronic kidney disease (CKD). Diagnosis of lupus nephritis (LN) is dependent on renal biopsy. Due to its invasiveness, repeat renal biopsy for monitoring disease activity is not recommended, thus creating a need for noninvasive and accurate biomarkers. Monocyte chemoattractant protein-1 (MCP-1) and tumour necrosis factor-like weak inducer of apoptosis (TWEAK) have been implicated in the pathogenesis of LN and are thus potential biomarkers for disease activity monitoring. Methods: In this study urinary

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31

Saint-Preux, Carlos. "Economic Feasibility of Chemical Spray Programs and the Effects of Nanoparticle Bactericides on the Management of Tomato Bacterial Spot." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1593677600328804.

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32

Nadeem, Athar 1955. "Induction of mild strains of pepper mottle virus by chemical mutagenesis and their efficacy in cross protection." Thesis, The University of Arizona, 1992. http://hdl.handle.net/10150/278085.

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Crude sap of pepper Capsicum frutescens L. 'NM 6-4' infected with pepper mottle virus (PeMV) was treated with nitrous acid to induce mutants. Twelve isolates were selected from two distinct types of local lesions on Capsicum frutescens L. 'tabasco'. Of the twelve isolates, four produced mild symptoms on both pepper foliage and fruit. When these mild isolates were inoculated to pepper plants and subsequently challenged with wild type PeMV, they protected the plants from the severe effect of the wild type isolate. Marketable pepper fruit from these protected plants was not significantly differen

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33

Stewart, Rachel M. "The prevalence of eating disorder pathology and inadequate diets among Division I female collegiate athletes." Morgantown, W. Va. : [West Virginia University Libraries], 2002. http://etd.wvu.edu/templates/showETD.cfm?recnum=2436.

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Thesis (M.S.)--West Virginia University, 2002.<br>Title from document title page. Document formatted into pages; contains v, 46 p. : ill. Includes abstract. Includes bibliographical references (p. 44-46).

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Jalali, Sefid Dashti Mahjoubeh. "Development of a pathology-supported genetic test for improved clinical management of patients diagnosed with multiple sclerosis." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5407.

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Thesis (MScMedSc (Pathology. Chemical Pathology))--University of Stellenbosch, 2010.<br>Bibliography<br>ENGLISH ABSTRACT: The aetiology of multiple sclerosis (MS) remains largely unknown, due to its multifactorial nature with environmental and genetic factors contributing to the risk. Several investigations highlighted the important role of the genetic component influencing disease susceptibility and progression. In the present study genetic variations in the MTHFR (1298 A>C and 677 C>T) and HFE (845 G>A) genes previously, shown to affect folate and iron metabolism respectively, were studied

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Li, Weihua 1965. "Glandular and gastrointestinal (GI) amyloidosis and the chemical nature of GI amyloid in alveolar hydatid cyst infected mice." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22760.

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Patients with systemic amyloidosis frequently show gastrointestinal (GI) tract and adrenal gland involvement with amyloid. However, the evolution of amyloid-related pathological changes in humans is unknown. Conversely, although GI amyloidosis has been described in casein-stimulated mice, the chemical nature of the GI amyloid have not been carried out. Here we report studies on these and related aspects using alveolar hydatid cyst (AHC)-infected mouse model of reactive amyloidosis. Paraffin sections from the adrenal gland, stomach, small and large intestine were obtained from AHC-mice at diffe

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36

Germishuys, Jurie J. "Free light chains in patients with HIV: establishing local reference ranges and their association with stage of disease, chronic antigen stimulation and the effect of Haart." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/20173.

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Thesis (MMedSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Background: Serum free light chains (FLC) are associated with imbalances in heavy and light chain production. Abnormal FLC ratios have been associated with risk of progression in certain diseases. Automated assays are available for their determination and they are used in the followup and management of patients with monoclonal gammopathies. Acceptable imprecision, specificity, accuracy and reproducibility between reagent batches is required to prevent under- or overestimation. Method validation is a standard process in

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37

Rutter, Allison. "An NMR study of human brain tumours: Phosphorus chemical shift imaging in vivo and high resolution proton spectroscopy of biopsy samples." Thesis, University of Ottawa (Canada), 1992. http://hdl.handle.net/10393/7681.

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Human brain tumours have been investigated using proton and phosphorus nuclear magnetic resonance (NMR). The localization technique, chemical shift imaging has been used to obtain phosphorus spectra of tumours in vivo. High resolution proton spectroscopy has been performed on biopsy samples and extracts of human brain tumours. To obtain the localized phosphorus spectra, one dimensional chemical shift imaging with a surface coil was adapted to a 1.5 T Siemens Magnetom imager. The ratios of areas, PDE/ATP and PME/ATP were found to be higher in glioblastomas and astrocytomas than in normal brain.

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Fernandez, Mercedes 1963. "EEG measures of subjects with idiopathic chemical sensitivity: A test of the sensitization model." Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/282608.

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This study tested the sensitization model proposed by Bell, Miller & Schwartz (1992) to study multiple chemical sensitivity (MCS). The sensitization models indicates that a traumatic event which elicits a strong response can sensitize limbic and/or mesolimbic pathways and future less intense trauma or stimuli, in the same or different modality, can elicit an amplified response. Three groups of subjects were tested: (1) women who reported chemical sensitivity; (2) sexually abused women without chemical sensitivity; and (3) healthy woman without chemical sensitivity nor sexual abuse history. All

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Seleka, Mpho Maria. "Development of selective real-time PCR (SPCR) asays for the detection of K103N resistance mutation in minor HIV-1 populations." Thesis, Stellenbosch : Stellenbosch University, 2011. http://hdl.handle.net/10019.1/18028.

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Thesis (MScMedSc)--Stellenbosch University, 2011.<br>ENGLISH ABSTRACT: Background: The conventional sequence analysis is the most common method used for the detection of drug-resistant mutants. Due to its sensitivity limitations, it is unable to detect these mutants when comprising less than 20% (minor populations) of the total virus population in a sample. However, real-time PCR-based assays offer a rapid, sensitive, specific and easy detection and quantification of such mutants. The HIV-1 variants harbouring the K103N mutation are associated with resistance to nevirapine (NVP) and efavirenz

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Mak, Miu, and 麥苗. "Chemical pathology analysis of inborn errors of metabolism for expanded newborn screening in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48540924.

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Inborn errors of metabolism (IEM) are under international spotlight because of the recent tremendous development in expanded newborn screening (NBS) and molecular genetics. IEM is a difficult subject involving more than 1,000 different disorders with protean clinical presentations and complicated diagnostic pathways. Cumulative incidence of IEM was reported up to 1 in 800. Patients can be affected in any ages. High clinical suspicion alone is not sufficient to reduce morbidities and mortalities. Notably, some IEM are amenable to treatment with promising outcome. Local data regarding the dise

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Marouelli, Waldir Aparecido 1958. "Improving chemigation efficacy by controlling droplet size distribution of oil-based pesticides." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282265.

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For chemigation of nonsoluble pesticides, small oil-pesticides droplets (dmax tend to wash-off from foliage while large droplets tend to stick. Large droplets (dmax are buoyant, tend to rise in the irrigation pipeline and exit at the beginning of the pipeline; thus, uniformity and efficacy are poor. For this research, a new chemigation system was proposed. The system removes water from the irrigation pipeline, injects the oil-pesticide into the water stream, increases dispersion velocity in successively smaller tubing diameters, and finally injects the dispersion back into the irrigation pipel

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Al-Asmari, Abdulrahman Khazim. "Production and assessment of ovine antivenoms for the treatment of snake envenoming in Saudi Arabia." Thesis, Queen Mary, University of London, 1996. http://qmro.qmul.ac.uk/xmlui/handle/123456789/24950.

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Venoms from the most poisonous snakes found in Saudi Arabia were assessed for their physical and chemical characteristics and for their enzymatic and biological activities. Venom from Atractaspis microlepidota was the most lethal in mice followed by the elapids Naja haje arabica and Walterinnesia aegyptia. Among the vipers, Cerastes cerastes venom was the most lethal whereas the remainder (Echis pyramidum, Echis coloratus and Bitfis arietans) showed similar but lower lethality. Antivenoms were raised in sheep by immunising with a low dose of venom (0.5mg) which was then doubled every four week

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43

Flanagan, Colleen A. "Gonadotropin releasing hormone receptor ligand interactions." Doctoral thesis, University of Cape Town, 1995. http://hdl.handle.net/11427/27029.

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The decapeptide, gonadotropin releasing hormone (GnRH), is the central regulator of reproductive function. It binds to receptors on the gonadotrope cells of the pituitary and stimulates release of luteinizing hormone (LH) and follicle stimulating hormone (FSH). Eleven different structural forms of GnRH have now been identified in various animal species. Chimaeric analogues of some of the variant forms of GnRH were synthesized in order to study the functional significance of the most common amino acid substitutions, which occur in positions 5, 7 and 8. Peptide binding affinities for sheep and r

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44

James, Adrian. "The influence of intelligence-led policing models on investigative policy and practice in mainstream policing 1993-2007 : division, resistance and investigative orthodoxy." Thesis, London School of Economics and Political Science (University of London), 2011. http://etheses.lse.ac.uk/221/.

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This research addressed the question ‘What is the National Intelligence Model (NIM), why did it emerge and how has it influenced police organisational structures and investigative practice’? The NIM embodied the apotheosis of intelligence-led policing (ILP) policy in Britain. Allied to the pre-existing ‘intelligence cycle’, it represented an eclectic ‘pick n’ mix’ of strategies that aimed to deliver effectiveness and ‘best value’ in policing. Sir David Phillips, sponsor of the model and President of the Association of Chief Police Officers (ACPO), during a key period in its development, wanted

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Pithey, Anne Louise. "Autocrine regulation of gonadotropin-releasing hormone in immortalized hypothalamic GT1-7 neurons." Master's thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/27030.

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The existence of an ultrashort feedback mechanism regulating GnRH secretion has been supported from in vivo and in vitro studies. However, the complex synaptic connections of GnRH neurons with other neural elements made it difficult to determine whether the regulation was mediated by direct actions on the GnRH neurons or through actions on other interneurons. The recent development of the GnRH-secreting neuronal cell line, GT1, provided a model system for the study of neural regulation of a pure population of GnRH neurons. The present studies utilized GT1 -7 cells to investigate whether GnRH (

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Harrison, Kenneth Louis. "Development and assessment of avian and ovine antivenoms for European viper venoms." Thesis, Queen Mary, University of London, 2004. http://qmro.qmul.ac.uk/xmlui/handle/123456789/28570.

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This research was undertaken in order to design techniques and processes that enable the manufacture of effective antivenoms. To prepare a broad specificity anti venom for European vipers from chicken yolk it was first necessary to develop a simple effective method to extract avian immunoglobulin (lgY). A specific fluoroimmunoassay was developed to monitor IgY recovery and serum IgY levels in immunised hens. The most promising extraction methods from the literature were compared using a triglyceride kit to monitor lipoprotein removed and SDS-PAGE and ELISA to monitor purity and activity respec

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Shires, Karen Lesley. "Citrulline metabolism in cultured fibroblasts : citrullinemia analysis and nitric oxide production." Master's thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/27031.

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A citrullinemic fibroblast cell line was used in this study to investigate two biochemical pathways involving citrulline. In the first section, the genetic mutation responsible for the argininosuccinate synthetase (-ASS) deficiency (1-5% activity) in this cell line was investigated. PCR analysis of the ASS cDNA revealed that the mRNA coding region (1236bp) was intact, showing no signs of major rearrangements. The ASS cDNA (1307bp) was cloned and sequenced and showed the presence of a single base mutation at position 1045bp, which represented a G->A transition. This mutation resulted in a glyci

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Pheiffer, Carmen P. "The role of steroidogenic factor-1 (SF-1) in gonadotropin-releasing hormone (GnRH) receptor gene regulation." Master's thesis, University of Cape Town, 1998. http://hdl.handle.net/11427/26970.

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Gonadotropin releasing hormone (GnRH) is a key reproductive hormone in vertebrates and exerts its effects via the GnRH receptor (GnRHR) to result in the synthesis and release of the gonadotropin hormones in the pituitary gonadotrope cells. GnRHR expression is likely to be regulated in a tissue- and cell- specific manner. A variety of hormones, including GnRH itself, estrogen, progesterone, inhibin, and testosterone have been shown to regulate GnRHR expression. Steroidogenic Factor-1 (SF-1), a member of the orphan nuclear receptor transcription factor family, regulates the expression of both th

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Ohland, Derek Paul. "Systematics of cetaceans using restriction site mapping of mitochondrial DNA." Master's thesis, University of Cape Town, 1992. http://hdl.handle.net/11427/27119.

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A phylogenetic study of eleven cetaceans was undertaken using Restriction Endonuclease Maps (RSM) of mitochondrial DNA (mtDNA). One species from the suborder mysticeti (baleen whales) was sampled, and of the ten odontocetes (toothed whales) sampled two were from the family Ziphiidae (beaked whales) and eight were from the family Delphinidae (dolphins) (each representing a different genus). The primarily opportunistically obtained (i.e. from strandings or accidental death in commercial trawl nets) heart tissue generally yielded high quantities of mtDNA which is needed for double digest fragment

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Tiemensma, Marianne. "Sudden and unexpected deaths in adults : an investigation of cases reported to Tygerberg Forensic Pathology Services from January 2001 - December 2005." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5179.

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Thesis (MMed (Pathology. Forensic Medicine))--University of Stellenbosch, 2010.<br>Bibliography<br>ENGLISH ABSTRACT: Background - The workload of the forensic pathologist and Forensic Pathology Services staff is increased by the referral of potentially unnecessary natural cases to the Forensic Pathology Services. The primary aims of the medico-legal autopsy are limited to establishing a cause of death in presumed unnatural cases, and to exclude criminality or negligence. Objective – To determine the final outcomes of forensic post-mortem examinations in “sudden and unexpected” adult deat

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