Relevant bibliographies by topics / Pathovar

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Academic literature on the topic 'Pathovar'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "Pathovar"

1

Kant, Pragya, Mario Fruzangohar, Rachel Mann, Brendan Rodoni, Grant Hollaway, and Garry Rosewarne. "Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Pseudomonas syringae Pathovars pisi and syringae." Agriculture 11, no. 9 (September 13, 2021): 875. http://dx.doi.org/10.3390/agriculture11090875.

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Pseudomonas syringae causes bacterial blight (BB) disease worldwide on economically important fruit and vegetable crops including field pea (Pisum sativum L.). The two pathovars responsible for BB in field pea are Pseudomonas syringae pathovar pisi (Psp) and syringae (Pss). In the field, both pathovars cause indistinguishable symptoms on field pea and require laboratory diagnosis to determine the causal pathovar. To aid in-field and laboratory diagnosis, accurate, and robust loop-mediated isothermal amplification (LAMP) assays for Psp and Pss were developed. The assays were able to detect Psp or Pss on live or heat-killed bacterial cells, plant exudates, seeds, and DNA extracts with no inhibitory effects. The two specific LAMP assays developed detected Psp and Pss accurately in less than 20 min and no cross-reaction was observed with 18 strains of closely related species of Pseudomonas syringae. Compared to the conventional PCR assays, the two LAMP assays were equally specific but have advantages of producing quicker and visual live results, enabling early detection and differentiation of Psp and Pss. Our results suggested a potential use of LAMP assays for laboratory testing and can be applied for in-field surveys.
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Weingart, H., B. Völksch, and M. S. Ullrich. "Comparison of Ethylene Production by Pseudomonas syringae and Ralstonia solanacearum." Phytopathology® 89, no. 5 (May 1999): 360–65. http://dx.doi.org/10.1094/phyto.1999.89.5.360.

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Strains of Pseudomonas syringae pv. pisi and Ralstonia solanacearum produced ethylene at rates 20- and 200-fold lower, respectively, than strains of P. syringae pvs. cannabina, glycinea, phaseolicola, and sesami. In the current study, we investigated which ethylene biosynthetic pathways were used by P. syringae pv. pisi and R. solanacearum. Neither the activity of an ethylene-forming enzyme nor a corresponding efe gene homolog could be detected in R. solanacearum, suggesting synthesis of ethylene via 2-keto-4-methyl-thiobutyric acid. In contrast, 2-oxoglutarate-dependent ethylene formation was observed with P. syringae pv. pisi, and Southern blot hybridization revealed the presence of an efe homolog in this pathovar. The efe genes from P. syringae pvs. cannabina, glycinea, phaseolicola, pisi, and sesami were sequenced. Nucleotide sequence comparisons indicated that the efe gene in pv. pisi was not as highly conserved as it was in other P. syringae pathovars. The pv. pisi efe homolog showed numerous nucleotide substitutions and a deletion of 13 amino acids at the C-terminus of the predicted gene product. These sequence alterations might account for the lower rate of ethylene production by this pathovar. All ethylene-producing P. syringae pathovars were virulent on bush bean plants. The overlapping host range of these pathovars suggests that horizontal transfer of the efe gene may have occurred among bacteria inhabiting the same host.
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Colhoun, K. M., R. C. Butler, and M. V. Marroni. "Pruning date affects bacterial canker of sweet cherry." New Zealand Plant Protection 68 (January 8, 2015): 448. http://dx.doi.org/10.30843/nzpp.2015.68.5858.

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The effect of pruning date on the development of canker lesions caused by Pseudomonas syringae pv syringae (Pss) and Ps pv morsprunorum (Psm) was investigated in a field trial using three sweet cherry cultivars with or without Pss and Psm inoculation Four weeks after treatment the percentage of twigs showing bacterial canker lesions and the size of lesions were recorded Overall the percentage of twigs showing lesions did not vary between cultivars but did vary with the pruning date and between pathovars Uninoculated branches pruned in February 2014 did not develop canker lesions A moderate proportion (40) showed lesions when inoculated with Psm and 100 of twigs showed necrosis when inoculated with Pss However for later pruning dates (April and July 2014) the percentage of twigs showing lesions declined progressively for twigs inoculated with both pathovars up to the September pruning Thereafter a sharp increase was observed with nearly 100 of twigs showing necrosis after the final January 2015 pruning For branches that showed necrosis lesion size varied strongly with cultivar pruning time and pathovar Lesion size tended to be smaller with later pruning but the pattern varied considerably with pathovar and cultivar The significance of these findings in relation to bacterial canker management is discussed
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Donahoo, R. S., J. B. Jones, G. H. Lacy, V. K. Stromberg, and D. J. Norman. "Genetic Analyses of Xanthomonas axonopodis pv. dieffenbachiae Strains Reveal Distinct Phylogenetic Groups." Phytopathology® 103, no. 3 (March 2013): 237–44. http://dx.doi.org/10.1094/phyto-08-12-0191-r.

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A comprehensive analysis of 175 Xanthomonas axonopodis pv. dieffenbachiae strains isolated from 10 Araceae hosts was done to identify pathogen variation. The strains were subjected to repetitive extragenic palindromic sequence polymerase chain reaction and four major phylogenetic clusters were generated. A subset of 40 strains isolated from Anthurium, Dieffenbachia, and Syngonium was further defined by amplified fragment length polymorphism and fatty acid methyl ester analysis and the same four phylogenetic clusters were observed. Comparison of representative strains in the first three clusters using DNA-DNA hybridization and multilocus sequence analysis supports the previous reclassification of strains in cluster I, including the X. axonopodis pv. dieffenbachiae pathovar reference strain (LMG695), to X. citri. Our research findings indicate that strains in cluster I, isolated primarily from anthurium, probably represent an undescribed pathovar. Other phylogenetic subclusters consisting primarily of strains isolated from xanthosoma and philodendron in clusters III and IV, respectively, may yet represent other undescribed species or pathovars of Xanthomonas.
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Schaad, N. W., A. K. Vidaver, G. H. Lacy, K. Rudolph, and J. B. Jones. "Evaluation of Proposed Amended Names of Several Pseudomonads and Xanthomonads and Recommendations." Phytopathology® 90, no. 3 (March 2000): 208–13. http://dx.doi.org/10.1094/phyto.2000.90.3.208.

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In 1980, over 90% of all plant-pathogenic pseudomonads and xanthomonads were lumped into Pseudomonas syringae and Xanthomonas campestris, respectively, as pathovars. The term “pathovar” was created to preserve the name of plant pathogens, but has no official standing in nomenclature. Proposals to elevate and rename several pathovars of the genera Pseudomonas and Xanthomonas to the rank of species has caused great confusion in the literature. We believe the following changes have merit and expect to adopt them for publication in a future American Phytopathological Society Laboratory Guide for Identification of Plant Pathogenic Bacteria. Upon review of published data and the Rules of The International Code of Nomenclature of Bacteria, we make the following recommendations. We reject the proposal to change the name of P. syringae pvs. phaseolicola and glycinea to P. savastanoi pvs. phaseolicola and glycinea, respectively, because both pathogens are easily differentiated phenotypically from pv. savastanoi and convincing genetic data to support such a change are lacking. We accept the elevation of P. syringae pv. savastanoi to the rank of species. We accept the reinstatement of X. oryzae to the rank of species with the inclusion of X. oryzicola as a pathovar of X. oryzae and we accept the species X. populi. We agree with the elevation of the pvs. cassavae, cucurbitae, hyacinthi, pisi, and translucens to the rank of species but not pvs. melonis, theicola, and vesicatoria type B. We recommend that all type A X. vesicatoria be retained as X. campestris pv. vesicatoria and all type B X. vesicatoria be named X. exitiosa. We reject the newly proposed epithets arboricola, bromi, codiaei (poinsettiicola type B), hortorum, sacchari, and vasicola and the transfer of many pathovars of X. campestris to X. axonopodis. The proposed pathovars of X. axonopodis should be retained as pathovars of X. campestris.
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Zhao, Youfu, John P. Damicone, David H. Demezas, and Carol L. Bender. "Bacterial Leaf Spot Diseases of Leafy Crucifers in Oklahoma Caused by Pathovars of Xanthomonas campestris." Plant Disease 84, no. 9 (September 2000): 1008–14. http://dx.doi.org/10.1094/pdis.2000.84.9.1008.

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Fields of kale, spinach mustard, and turnip were severely damaged by bacterial leaf spots during 1994 to 1996. Symptoms included circular to angular necrotic lesions with yellow halos and water-soaking on the abaxial leaf surface. Yellow, mucoid strains isolated from leaf spots were identified as Xanthomonas campestris using Biolog. Four strains caused black lesions on stems of cabbage seedlings in an excised cotyledon assay, leaf spots and sunken dark lesions on petioles of spray-inoculated crucifers, and leaf spots on spray-inoculated tomato. These strains were classified as X. campestris pv. armoraciae. Most other strains from leafy crucifers and all strains from a cabbage field caused black rot in the cotyledon assay and in spray-inoculations. Many of these strains also caused leaf spots on collard and kale but not stem and petiole lesions. The strains causing black rot were classified as X. campestris pv. campestris. Cluster analysis of Biolog profiles yielded a small group that contained local strains of both pathovars, and a large group comprised of reference and local strains of each pathovar, and some local, nonpathogenic strains. Five fingerprint groups were identified by rep-polymerase chain reaction using the BOXA1R primer. Local and reference strains of each pathovar occurred in two of the groups. Two pathovars of X. campestris are involved in the leaf spot diseases. Both pathovars were recovered within several fields, and also were recovered along with Pseudomonas syringae pv. maculicola. This is the first report of Xanthomonas leaf spot caused by X. campestris pv. armoraciae in Oklahoma.
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Rasko, David A., M. J. Rosovitz, Garry S. A. Myers, Emmanuel F. Mongodin, W. Florian Fricke, Pawel Gajer, Jonathan Crabtree, et al. "The Pangenome Structure of Escherichia coli: Comparative Genomic Analysis of E. coli Commensal and Pathogenic Isolates." Journal of Bacteriology 190, no. 20 (August 1, 2008): 6881–93. http://dx.doi.org/10.1128/jb.00619-08.

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ABSTRACT Whole-genome sequencing has been skewed toward bacterial pathogens as a consequence of the prioritization of medical and veterinary diseases. However, it is becoming clear that in order to accurately measure genetic variation within and between pathogenic groups, multiple isolates, as well as commensal species, must be sequenced. This study examined the pangenomic content of Escherichia coli. Six distinct E. coli pathovars can be distinguished using molecular or phenotypic markers, but only two of the six pathovars have been subjected to any genome sequencing previously. Thus, this report provides a seminal description of the genomic contents and unique features of three unsequenced pathovars, enterotoxigenic E. coli, enteropathogenic E. coli, and enteroaggregative E. coli. We also determined the first genome sequence of a human commensal E. coli isolate, E. coli HS, which will undoubtedly provide a new baseline from which workers can examine the evolution of pathogenic E. coli. Comparison of 17 E. coli genomes, 8 of which are new, resulted in identification of ∼2,200 genes conserved in all isolates. We were also able to identify genes that were isolate and pathovar specific. Fewer pathovar-specific genes were identified than anticipated, suggesting that each isolate may have independently developed virulence capabilities. Pangenome calculations indicate that E. coli genomic diversity represents an open pangenome model containing a reservoir of more than 13,000 genes, many of which may be uncharacterized but important virulence factors. This comparative study of the species E. coli, while descriptive, should provide the basis for future functional work on this important group of pathogens.
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Chen, Yong-Fang, Yan-Ni Yin, Xiao-Mei Zhang, and Jian-Hua Guo. "Curtobacterium flaccumfaciens pv. beticola, A New Pathovar of Pathogens in Sugar Beet." Plant Disease 91, no. 6 (June 2007): 677–84. http://dx.doi.org/10.1094/pdis-91-6-0677.

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Bacterial leaf spot of sugar beet was first discovered in 1995 in Inner Mongolia of China. The pathogen was shown to be a bacterium with properties of gram-positive bacteria: small irregular rods, lateral flagella, aerobic, and catalase-positive. The colonies of sugar beet strains produced a pale-yellow pigment. The optimum temperature for the bacteria to grow was 24 to 27°C. The bacteria could utilize a wide range of organic compounds, including hydrolyzed casein, starch, esculin and Tween 80, and released H2S from cysteine, cystine, and Na2S2O3·5H2O, but could not produce urease, oxidase, or indole. The cell wall peptidoglycan was based on ornithine (type B2β). The predominant menaquinone was MK-9. Polar lipids contained several glycosyldiacyl-glycerols. The DNA G+C content of a type strain of the new pathovar, T30T, was 67.5%. DNA-DNA homology between T30T and Curtobacterium flaccumfaciens pv. flaccumfaciens (International Collection of Micro-Organisms from Plants, New Zealand [ICMP] 2584) was 70.1%. The new pathovar and C. flaccumfaciens pv. flaccumfaciens had 99.9% identity in DNA sequence of 16S rRNA. Close genetic relatedness was observed for the representatives of the species Curtobacterium flaccumfaciens, but a low level of similarity between the different pathovars was found. Based on these physiological, biochemical, chemotaxonomic, phylogenetic, and genetic characteristics, we demonstrate that the pathogen represents a new pathovar of C. flaccumfaciens, for which we propose the name Curtobacterium flaccumfaciens pv. beticola pv. nov. The type strain is T30T (=ATCC BAA-144T).
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Pastoschuk, A., M. Kovalenko, and L. Skivka. "Antioxidant reactions in winter wheat seedlings of different cultivars exposed to the Pseudomonas syringae and its lipopolysaccharides in vitro." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 84, no. 1 (2021): 61–66. http://dx.doi.org/10.17721/1728_2748.2021.84.61-66.

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Pseudomonas syringae is the most common phytopathogenic bacterium with a wide range of target plants, which include important cereals such as wheat. One of the main pathogens of bacterial diseases of wheat is Pseudomonas syringae pv. atrofaciens. In some countries, wheat yield losses caused by this phytopathogenic bacterium reach 50%. Currently, the taxonomy of P. syringae includes more than 50 pathovars with varying degrees of adaptation to wheat lesions. One of them is Pseudomonas syringae pv. сoronafaciens. P. syringae pv. Coronafaciens is non-host pathogen for wheat. However, the infectionsof a wide range of crops, including wheat, with this pathogen attracts the attention of both researchers and specialiss of the agro-industrial complex. The study of the mechanisms of wheat resistance to host and non-host pathovars of P. syringae is of great interest, both in terms of in-depth study of the pathogen and in the perspective of selection of bacterial disease-resistant varieties of this strategically important grain crop for Ukraine. The aim of the study was to compare the antioxidant reactions of wheat seedlings of different winter wheat varieties under the grain exposition to P. syringae of different pathovars and their lipopolysaccharides (LPS). It was found that reactive oxygen species generation, as a mechanism of plant immune protection against phytopathogenic pseudomonads, is equally activated in the case of exposure to both host and nonhost pathovars and to a lesser extent in the case of the exposure with LPS of both pathovars. In grains of Favoritka variety (most sensitive to phytopathogenic pseudomonads) exposed to host pathovar, significant activation of antioxidant enzymes was observed. Exposure to the non-host pathovar causes sharp proline accumulation. Thus, the sensitivity of wheat seedlings to phytopathogenic host and non-host pathovars of phytopathogenic pseudomonads largely depends on the balanced functioning of the antioxidant defense system. Taken together, these data indicate the wheat cell oxidative metabolism as a target for selection of varieties resistant to phytopathogenic bacteria.
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Morris, Cindy E., Catherine Glaux, Xavier Latour, Louis Gardan, Régine Samson, and Michel Pitrat. "The Relationship of Host Range, Physiology, and Genotype to Virulence on Cantaloupe in Pseudomonas syringae from Cantaloupe Blight Epidemics in France." Phytopathology® 90, no. 6 (June 2000): 636–46. http://dx.doi.org/10.1094/phyto.2000.90.6.636.

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In 1993, a bacterial blight caused important losses of cantaloupe (Cucumis melo var. cantalupensis) in southwestern France and has now been reported in all cantaloupe-growing regions of France. The causal agent of this blight is Pseudomonas syringae, although on a worldwide basis this bacterium has not been a major pathogen of melon for over 50 years. To identify the pathovar of the cantaloupe pathogen, we employed biochemical tests, plasmid and chromosomal profiling, and host range studies for 23 strains from cantaloupe and 47 reference strains of 14 pathovars of P. syringae. Numerical analysis of 119 traits, serological typing, syringomycin production, and BOX-polymerase chain reaction profiles did not allow us to differentiate among pathovars related to P. syringae pv. syringae. Host range studies of cantaloupe and references strains on 18 plant species showed that virulence to sugar beet was a common feature of strains virulent on cantaloupe, but was not common to strains avirulent on cantaloupe. Virulence to other species of plants varied among strains, but the overall extent of the host range was proportional to aggressiveness to cantaloupe. We propose that the strains attacking cantaloupe in France be considered P. syringae pv. aptata and that adequate host range testing may reveal that this pathovar is the cause of cantaloupe blight reported in other parts of the world.
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Dissertations / Theses on the topic "Pathovar"

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Malik, A. N. "Genetic studies with Pseudomonas syringae pathovar pisi." Thesis, University of Greenwich, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354390.

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BERTHIER, YVETTE. "Caracterisation de xanthomonas campestris pathovar dieffenbachiae : etude serologique et moleculaire." Paris 11, 1993. http://www.theses.fr/1993PA112289.

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Xanthomonas campestris pv dieffenbachiae, (x. C. D. ) pathogene de plantes de la famille des aracees est un facteur limitant de la production d'anthurium dans de nombreuses regions tropicales. Il appartient a l'espece campestris qui regroupe plus de 140 pathovars distincts par leur hote d'origine. Afin de clarifier la position taxonomique de ce groupe et d'etudier sa variabilite interne nous avons utilise deux sondes: la sonde rarn 16+23s d'e. Coli marquee a l'acetylaminofluorene et une sonde repetee isolee du genome de x. Campestris pv. Dieffenbachiae, is 1051. Par ribotypage cinquante-six souches de x. Campestris pv. Dieffenbachiae representatives de la variabilite de ce pathovar ont ete analysees. Huit profils ont ete obtenus: 4 correspondent aux souches d'anthurium, les 4 autres representent des souches d'autres aracees. Le profil majoritaire regroupe 34 des 43 souches d'anthurium analysees. Ces profils ont ete compares a ceux de 4 souches de pseudomonas, 3 souches de xylophilus ampelinus, 28 souches de differentes especes de xanthomonas, et 156 souches appartenant a 14 pathovars de l'espece campestris. Des profils majoritaires se degagent de l'analyse des souches de x. Albilineans, x. Campestris pv begoniae, malvacearum et manihotis. Une analyse de ces profils fait apparaitre le regroupement des pathovars de l'espece campestris. L'etude de la variabilite genetique a ete approfondie avec la sonde is 1051. Cette sonde est une sequence d'insertion de la famille des is 5. C'est un element de 1158 pb borne par 2 sequences repetees inversees de 15 pb. Les profils rflp obtenus permettent de distinguer les origines geographiques et les plantes hotes. L'is 1051 est un element genetique assez conserve au sein des xanthomonas. Des profils d'hybridation ont ete obtenus avec des souches d'autres especes (x. Fragariae, x. Axonopolis, x. Oryzae) et avec des souches appartenant a 11 pathovars de l'espece campestris. La detection de la bacterie a ete etudiee par voies serologique et moleculaire. Un serum polyclonal a ete produit. Il detecte en elisa les souches de x. Campestris dieffenbachiae d'anthurium avec une sensibilite de 10#4b/ml. Des oligonucleotides internes a is 1051 ont ete testes pour la detection par pcr. Le couple y5 y9 amplifie 1 a 2 ng d'adn de souches isolees d'anthurium provenant d'origines geographiques differentes et representatives des differents profils d'hybridation
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Atherton, G. T. "The genetics of host specificity and pathogenicity of Pseudomonas syringae pathovar pisi." Thesis, University of the West of England, Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379473.

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Smith, Samantha Gillian. "An investigation of aspects of oxidative stress in Xanthomonas campestris pathovar campestris." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296866.

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Mur, Luis Alejandro Jose. "The molecular genetics of race 2 specificity in Pseudomonas syringae pathovar pisi." Thesis, University of the West of England, Bristol, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303616.

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Santos, Kelly. "Caracterização bioquimica de aminopeptidases de Xylella fastidiosa e Xanthomonas axonopodis pathovar citri." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314240.

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Orientador: Francisco Javier Medrano Martin
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-08T07:29:55Z (GMT). No. of bitstreams: 1 Santos_Kelly_D.pdf: 4767617 bytes, checksum: 886f6b5d543544a3913da5d9e5dae0bb (MD5) Previous issue date: 2006
Resumo: Aminopeptidases realizam a clivagem de resíduos de aminoácidos de peptídios e proteínas. Elas estão presentes em todos os organismos e desempenham importantes papéis em processamento de alimentos, maturação de proteínas pela eliminação do resíduo de metionina Nterminal, patogenicidade e muitos outros processos celulares. Neste trabalho foi realizada a clonagem, expressão, purificação e caracterização de uma prolina iminopeptidase de Xylella fastidiosa(Xf1510) e de uma aminopeptidase de Xanthomonasaxonopodispv. citri (Xac2987). Estes dois genes foram anotados como prováveis prolina iminopeptidases. Ambos foram clonados em vetor de expressão pET15b, expressados em Escherichiacoli, e purificados na fração solúvel em um passo por cromatografia a metal imobilizado (IMAC). Ensaios de atividade enzimática confirmaram que a Xf1510 é uma prolina iminopeptidase, e que a Xac2987 é uma aminopeptidase de amplo espectro e não uma prolina iminopeptidase como foi anotada no banco de dados, sendo capaz de catalisar a remoção de diferentes aminoácidos de substratos sintéticos. Os espectros de dicroísmo circular das enzimas mostram que ambas podem ser incluídas na família das a/ß a/ß hidrolases e que estão enoveladas. A proteína Xf1510 apresenta maior atividade na faixa de pH entre 7,5 e 8,5. A temperatura ótima para hidrólise de prolina foi 45ºC. O pH ótimo para a atividade enzimática da proteína Xac2987 foi encontrado na faixa de pH de 6,5 e 7,5; sendo o pH ótimo 6,6. Neste pH a temperatura ótima para a hidrólise de alanina foi encontrada à 40ºC. Estudos estruturais com relação ao pH e estabilidade térmica das proteínas foram acompanhados por dicroísmo circular. Estudos de desnaturação térmica e química indicam que as proteínas Xf1510 e Xac2987 apresentam estados intermediários antes de atingirem o desenovelamento máximo
Abstract: Aminopeptidases release the Nterminal amino acid residue from polypeptides and proteins. They are present in all organisms and play several important roles in food processing, maturation of proteins by elimination of the Nterminal methionine, pathogenicity and many other cellular processes. We report here, the cloning, expression, purification and characterization of a proline iminopeptidase from X.fastidiosa(Xf1510) and a broad specificity aminopeptidase from X.axonopodispv. citri(Xac2987). These two genes have been annotated as putative proline iminopeptidase. Both genes were cloned into the pET15b expression vector, expressed in Escherichia coli, and purified to apparent homogeneity in one step by IMAC. The protein was expressed in the soluble fraction and could be purified in one step by IMAC. Enzymatic assays confirmed Xf1510 as a PIP, and Xac2987 as a broad spectrum aminopeptidase, being able to catalyze the removal of different synthetic substrates. The circular dichroism spectrum allowed us to classify the proteins as part of the a/ß hydrolyses family. Structural studies with pH dependence and thermal stability were preformatted by circular dichroism. The Xf1510 protein presents greater activity in the range of pH between 7,5 and 8,5. The optimum temperature for prolina hydrolysis was 45ºC. The pH optimum for the enzymatic activity of the Xac2987 protein was found in the range of pH of 6,5 and 7,5; being pH optimum 6,6. In this pH the temperature for alanine hydrolysis was found to 40ºC. Structural studies with regard to pH and thermal stability of proteins had been followed by circular dichroism. Studies of thermal and chemical denaturation indicate that the proteins Xf1510 and Xac2987 present intermediate states before reaching the maximum unfolding
Doutorado
Bioquimica
Doutor em Biologia Funcional e Molecular
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Cunty, Amandine. "Caractérisation de Pseudomonas syringae pv. actinidiae l’agent responsable de l’émergence d’une épidémie de chancre bactérien du kiwi en France et description de Pseudomonas syringae pv. actinidifoliorum, agent causal de taches foliaires sur kiwi." Thesis, Angers, 2015. http://www.theses.fr/2015ANGE0018/document.

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La bactérie responsable de chancre sur bois, Pseudomonas syringae pv. actinidiae (Psa), a causé trois épidémies depuis les années 1980 et se décline en trois biovars. La plus récente et dévastatrice (causée par Psa biovar 3), a été détectée pour la première fois en 2008 en Italie et s’est rapidement répandue dans la majorité des pays producteurs de kiwi, dont en France en 2010. Nous avons analysé la diversité de 280 souches de P. syringae isolées de kiwi en France. La caractérisation biologique et l’analyse phylogénétique des souches par MLSA ont révélé que les biovars 1, 2 et 3 appartenaient à une même lignée génétique, groupant également P. s. pv. theae. Les souches de biovar 4 constituent un ensemble de 4 lignées génétiques distinctes qui ont été rassemblées au sein d’un nouveau pathovar (Pseudomonas syringae pv. actinidifoliorum (Psaf)). Ces souches sont caractérisées par une pathogénie réduite (taches foliaires mais pas de chancre). Cette nouvelle classification permet une meilleure gestion des épidémies de chancre bactérien du kiwi. Le développement d’un schéma MLVA composé de 11 VNTRs a permis d’étudier la structuration génétique de populations de Psa biovar 3, de révéler de la diversité au sein de ce pathovar et d’identifier l’origine italienne de l’épidémie en France. Le séquençage du génome de cinq souches de Psaf et la comparaison de ces séquences avec celles d’autres génomes de Psa et Psaf, disponibles sur NCBI, a permis le développement d’un nouvel outil de détection par PCR temps réel, plus spécifique de chaque biovar de Psa et de Psaf. La MLVA et la PCR temps réel développées ici contribueront à l’amélioration de la surveillance de Psa dans le monde
The causal agent of bacterial canker, Pseudomonas syringae pv. actinidiae (Psa),has been responsible ofthree epidemics since 1980’s.Psa is divided in three biovars. The most recent and severe outbreak (causedby Psa biovar 3) was detected for the first time in Italy in 2008. It has spread very quickly in the main kiwifruit producing countries, as in France in 2010. We analyzed the diversity of 280 strains of P. syringae isolated fromkiwifruit in France. The biological characterization and the phylogenetic analysis of the strains by MLSArevealed that the biovars 1, 2 and 3 belong to the same genetic lineage, which include P. s. pv. theae, as well.The biovar 4 strains, which are structured in 4 distinct genetic lineages, have been grouped in a new pathovar(Pseudomonas syringae pv. actinidifoliorum (Psaf)). These strains are characterized by a low virulence (onlyspots on leaves and no canker on wood). This new classification help with the management of the bacterialkiwifruit canker outbreaks. The development of an MLVA scheme composed of 11 VNTRs allowed to studythe genetic structuration of Psa biovar 3 populations, to reveal the diversity within this pathovar and to identifythe Italian origin of the epidemic in France. The genome sequencing of five Psaf strains and the comparisonbetween these sequences and those of Psa and Psaf genomes already available on NCBI, allowed thedevelopment of a new detection tool by real-time PCR, specific of each Psa biovar and of Psaf. The MLVA andthe real-time PCR based detection technique developed here will contribute to the improvement of the monitoringof kiwifruit bacterial canker around the world
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Bavage, Adrian Daryl. "The plasmids of Pseudomonas syringae pathovar pisi : involvement in pathogenicity and race-specificity." Thesis, University of the West of England, Bristol, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278054.

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Orvis, Julie Dawn. "An investigation into the specificity of race 3 of Pseudomonas syringae pathovar pisi." Thesis, University of the West of England, Bristol, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315474.

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Gibbon, Marjorie Jane. "Molecular characterisation of an avirulence gene from race 2 of Pseudomonas syringae pathovar pisi." Thesis, University of the West of England, Bristol, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385396.

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Books on the topic "Pathovar"

1

Briggs, Georgett C. The identification and characterization of putative quorum sensing system in the phytopathogen pseudomonas syringae pathovar tomato DC3000. Ottawa: National Library of Canada, 2002.

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Orser, Cindy Sue. Cloning and expression of Pseudomonas syringae: Pathovar syringae and Erwinia herbicola ice nucleation genes in Escherichia coli. Ann Arbor: University Microfilms, 1986.

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Rudolph, K., T. J. Burr, J. W. Mansfield, D. Stead, A. Vivian, and J. von Kietzell, eds. Pseudomonas Syringae Pathovars and Related Pathogens. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7.

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Robinson, John W. Specificity in pathovars of Xanthomanas campestris. Birmingham: University of Birmingham, 1987.

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Fatmi, M'Barek. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Dordrecht: Springer Science + Business Media, B.V, 2008.

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Fatmi, M'Barek, Alan Collmer, Nicola Sante Iacobellis, John W. Mansfield, Jesus Murillo, Norman W. Schaad, and Matthias Ullrich, eds. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6901-7.

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Chao, Yü-chʻi. Molecular genetic characterization of a pathogenicity-attenuated mutant of Pseudomonas syringae pathovar syringae. 1991.

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Briggs, Georgette C. Identification and characterization of a putatative quorum sensing system in the phytopathogen pseudomonas syringae pathovar tomato DC3000. 2002, 2002.

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Rudolph, K., T. J. Burr, John W. Mansfield, David E. Stead, A. Vivian, and J. von Kietzell. Pseudomonas Syringae Pathovars and Related Pathogens. Kai Rudolph, 2012.

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1947-, Rudolph Klaus-Peter, ed. Pseudomonas syringae pathovars and related pathogens. Dordrecht: Kluwer Academic Publishers, 1997.

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Book chapters on the topic "Pathovar"

1

Schaad, N. W. "Problems with the Pathovar Concept." In Plant Pathogenic Bacteria, 783–85. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_171.

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Ercolani, G. L. "Practical Problems with the Pathovar Scheme in Plant Quarantine." In Plant Pathogenic Bacteria, 786–94. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_172.

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Vasinauskiene, Milda. "Occurrence and Control of a Pseudomonas Syringae Pathovar Causing Bacterial Lupine Blotch." In Developments in Plant Pathology, 623–28. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7_113.

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Arsenijevic, Momcilo, and Aleksa Obradovic. "A Pathovar of Pseudomonas syringae Causal Agent of Bacterial Leaf Spot and Blight of Pepper Transplants." In Developments in Plant Pathology, 61–66. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7_12.

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Sutra, L., M. Menard, J. Luisetti, J. P. Prunier, and L. Gardan. "Bacterial Canker of Wild Cherry Tree in France Caused by a new Pathovar of Pseudomonas syringae pv. avii (pv. nov.)." In Pseudomonas syringae and related pathogens, 675–79. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_74.

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Pruvost, O., L. Gagnevin, V. Glories, K. Mete, L. Gout, and A. Couteau. "Usefulness of IS1595 as a Molecular Tool for Epidemiological Typing of the Xanthomonas Pathovar mangiferaeindicae, Causal Agent of Mango Bacterial Black Spot." In Plant Pathogenic Bacteria, 138–40. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0003-1_28.

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Claflin, L. E. "Control of Pseudomonas syringae Pathovars." In Pseudomonas syringae and related pathogens, 423–30. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_46.

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El-Shouny, Wagih, Abd El-Raheem El-Shanshoury, El-Sayed A. Mostafa, Kerstin Wydra, and Klaus Rudolph. "Exopolysaccharides Produced by Pseudomonas syringae Pathovars." In Developments in Plant Pathology, 282–86. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7_52.

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Weingart, Helge, and Beate Völksch. "Ethylene Production by Pseudomonas syringae Pathovars." In Developments in Plant Pathology, 317–22. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7_59.

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Kucheryava, Nataliya, and Svitlana Votselko. "The Lectin Activity of Pseudomonas syringae Pathovars." In Developments in Plant Pathology, 358–63. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7_66.

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Conference papers on the topic "Pathovar"

1

Liu, Jiemin, and Laiquan Han. "QoS-Satisfied Pathover Scheme in FMIPv6 Environment." In 2008 International Conference on Computer Science and Software Engineering. IEEE, 2008. http://dx.doi.org/10.1109/csse.2008.1253.

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Jiemin Liu, Jingxin Dou, Hongxing Zou, and Yuan Gao. "Implementing aggressive Pathover with aware mSCTP extension in FMIPv6 networks." In IET 2nd International Conference on Wireless, Mobile and Multimedia Networks (ICWMMN 2008). IEE, 2008. http://dx.doi.org/10.1049/cp:20080992.

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Corvo, Alberto, Marc A. van Driel, and Michel A. Westenberg. "PathoVA: A visual analytics tool for pathology diagnosis and reporting." In 2017 IEEE Workshop on Visual Analytics in Healthcare (VAHC). IEEE, 2017. http://dx.doi.org/10.1109/vahc.2017.8387544.

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Khan, Md Kamrul Alam, and Shuva Paul. "A analytical study on Electrochemistry for PKL (Pathor Kuchi Leaf) electricity generation system." In 2013 IEEE Energytech. IEEE, 2013. http://dx.doi.org/10.1109/energytech.2013.6645296.

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Khan, K. A., Shahinul Islam, M. A. Saime, S. R. Rasel, and Sazzad Hossain. "A NEW AND SUSTAINABLE PKL ELECTRICITY." In Topics in Intelligent Computing and Industry Design. Volkson Press, 2021. http://dx.doi.org/10.26480/etit.02.2020.173.178.

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A new method of electricity generation based on Pathor Kuchi Leaf (Genus: Kalanchoe, Section: Bryophyllum) has been developed at the Department of Physics, Jagannath University, Dhaka- 1100, Bangladesh. This electricity generation method has several advantages for smart grid over the conventional electricity production. This sustainable method is likely to generate the employment at particularly in the rural areas of where grid electricity is absent. This research work reports an invention made on Pathor Kuchi Leaf (PKL) electric power plant to enhance the PKL electricity production. The efficiency of the PKl electricity production device, Short Circuit Current ( Isc ), Open circuit Voltage ( Voc ), Temperature effect of the PKL malt, pH of the PKL malt, Titratable acidity of the PKL malt, Generation of PKL electricity, Storage system of the PKL electricity, Particular utilization of PKL electricity, I-V characteristics of the PKL, Classification of PKL, Longevity of PKL malt for PKL electricity generation, Preparation of PKL electric unit cell, module, panel, arrays and the constituent elements of the PKL, Voltage regulation, Internal resistance of the cell and efficiency of the cell have been studied. The chemical reactions of the PKL electrochemical cell have also been studied. In experimental study, it is shown that the maximum efficiency of the PKL electricity production device is ≈ 34%, the pH of the PKL malt is ≈ 4.6(without water), pH of the PKL malt is ≈ 4.8 (with 10% solution), the titratable acidity of the PKL malt is ≈ 0.88%. Most of the results have been tabulated and graphically discussed.
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Khan, Md K. A., Akhlaqur Rahman, Shuva Paul, MD Siddikur Rahman, Md Tanvir Ahad, and Md Al Mamun. "An Investigation of Cell Efficiency of Pathor Kuchi Leaf (PKL) Cell for Electricity Generation." In 2019 International Symposium on Advanced Electrical and Communication Technologies (ISAECT). IEEE, 2019. http://dx.doi.org/10.1109/isaect47714.2019.9069676.

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Ohiduzzaman, Md, Rajia Sultana, Rajada Khatun, Shirin Akter, and K. A. Khan. "PORTABLE PKL POWERED LANTERN." In Topics in Intelligent Computing and Industry Design. Volkson Press, 2021. http://dx.doi.org/10.26480/etit.02.2020.179.183.

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It has been designed and developed the portable PKL (Pathor Kuchi Leaf) lantern for practical utilization for smart grid where grid electricity is absent. The PKL converter has been made with an insulated box and electrodes. The electrolytes were prepared by PKL extract. It was found that the portable lanterns were appropriate for PKL electric system. It is found that PKL Powered Lantern has been working well. The novelty of this research work shows that this type of work is very new one in the world. It can be used instead of solar PV sometimes. There are some advantages of this PKL systems compare to SPV (Solar Photo Voltaic) System. This PKL system can be used equally both for the rainy and night time. Whereas SPV system is not possible to use at the rainy and night time.
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Khan, Md Kamrul Alam, Shuva Paul, Md Siddikur Rahman, Ripon Kumar Kundu, Md Mahmudul Hasan, Mohammad Moniruzzaman, and Mohammad Al Mamun. "A study of performance analysis of PKL electricity generation parameters: (An experimental analysis on voltage regulation, capacity and energy efficiency of pathor kuchi leaf (PKL) electricity cell)." In 2016 IEEE 7th Power India International Conference (PIICON). IEEE, 2016. http://dx.doi.org/10.1109/poweri.2016.8077199.

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Khan, Kamrul Alam, Akhlaqur Rahman, Md Siddikur Rahman, Aniqa Tahsin, Kazi Md Jubyer, and Shuva Paul. "Performance analysis of electrical parameters of PKL electricity (An experimental analysis on discharge rates, capacity & discharge time, pulse performance and cycle life & deep discharge of Pathor Kuchi Leaf (PKL) electricity cell)." In 2016 IEEE Innovative Smart Grid Technologies - Asia (ISGT-Asia). IEEE, 2016. http://dx.doi.org/10.1109/isgt-asia.2016.7796442.

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