Relevant bibliographies by topics / PCR detekce

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'PCR detekce'

Author: Grafiati
Published: 4 June 2021
Last updated: 3 February 2022

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Journal articles on the topic "PCR detekce"

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Lee, Si Won, Yong-Gil Shin, Jin-Young Lee, Young-Suk Kim, Mi Hee Yang, and In-Cheol Choi. "Development of a diagnostic system to detect potato virus T using RT-PCR and nested PCR." CNU Journal of Agricultural Science 42, no. 2 (June 30, 2015): 99–103. http://dx.doi.org/10.7744/cnujas.2015.42.2.099.

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Li, Xiaoying, Lu Fu, Changlong Chen, Wangwang Sun, Yu Tian, and Hua Xie. "Characteristics and Rapid Diagnosis of Pectobacterium carotovorum ssp. Associated With Bacterial Soft Rot of Vegetables in China." Plant Disease 104, no. 4 (April 2020): 1158–66. http://dx.doi.org/10.1094/pdis-05-19-1033-re.

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Pectobacterium carotovorum, a causal agent of vegetable soft rot, contains three valid subspecies: P. carotovorum subsp. carotovorum (Pcc), P. carotovorum subsp. brasiliensis (Pcb), and P. carotovorum subsp. odoriferum (Pco). Using 16S rDNA sequencing and genus-specific PCR, we identified 72 P. carotovorum strains from Chinese cabbage, bok choy, and celery and assessed their pathogenicity on Chinese cabbage petioles and potato tubers. Based on phylogenetic analysis of pmrA sequences and confirmation by subspecies-specific PCR, the strains were divided into 18 Pcc, 29 Pco, and 25 Pcb. Several characteristic features were also assessed and supported the distinctiveness of the Pco strains. All P. carotovorum strains caused soft rot symptoms on Chinese cabbage and potato, but the Pco strains exhibited the greatest severity. We developed a conventional PCR and a quantitative PCR (qPCR) assay for the identification of Pco based on its specific srlE gene encoding sorbitol-specific phosphotransferase. These two methods could specifically amplify the expected products of 674 and 108 bp, respectively, from all of the Pco strains. The assays demonstrated high sensitivity and could detect as little as 1 and 100 pg/µl of bacterial genomic DNA, respectively. Both assays could also detect the pathogens directly from plant tissues infected with as little as 2.5 × 10−2 CFU/mg of Pco, even before external symptoms appeared. These assays constitute effective tools for disease diagnosis and the rapid identification of soft rot pathogens.
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Wąsowicz, Barbara, and Marianna Soroka. "Technika LAMP jako potencjalne narzędzie w analizach kryminalistycznych." Kosmos 67, no. 3 (October 24, 2018): 565–73. http://dx.doi.org/10.36921/kos.2018_2442.

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Amplifikacja w warunkach izotermicznych (technika LAMP) jest idealną odpowiedzią na rosnące zapotrzebowanie ze strony laboratoriów molekularnych, diagnostycznych, a także kryminalistycznych. Technika LAMP jest atrakcyjną alternatywą dla klasycznej reakcji PCR, a jej użycie pozwala na skrócenie czasu analizy nawet trzykrotnie. Polimerazy wykorzystywane do tego typu amplifikacji mają zdolność wypierania nici DNA, co eliminuje konieczność denaturacji matrycy. Dzięki użyciu kilku par starterów w reakcji LAMP, przeprowadzona amplifikacja jest bardziej specyficzna, a także w porównaniu do reakcji PCR pozwala na otrzymanie większej ilości produktu i jego detekcję bez użycia dodatkowychprocedur i sprzętu. Technika LAMP zyskuje znaczenie w takich dziedzinach jak: przestępstwa gospodarcze, diagnostyka ludzkich i zwierzęcych chorób zakaźnych, detekcja patogenów roślinnych o szerokim znaczeniu gospodarczym czy identyfikacja roślin GMO.
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Savva, D., and R. E. Holliman. "PCR to detect toxoplasma." Lancet 336, no. 8726 (November 1990): 1325. http://dx.doi.org/10.1016/0140-6736(90)93013-f.

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Sumíková, T., and A. Hanzalová. "Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat." Czech Journal of Genetics and Plant Breeding 46, No. 2 (June 29, 2010): 85–89. http://dx.doi.org/10.17221/32/2010-cjgpb.

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Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered in the Czech Republic. Gene Lr37 was detected in four tested cultivars (Bakfis, Biscay, Nicol, Mulan), gene Lr26 occurred only in one cultivar (Etela) and three cultivars (Clarus, Orlando and Rapsodia) were found to carry both these genes. Data obtained by PCR markers were compared with results of greenhouse and field tests. Seedling reactions of cultivars possessing Lr26 to seven different leaf rust isolates conformed to the results obtained by the marker analysis, however, there were found some discrepancies in the detections of Lr37, which could be detected in greenhouse seedling tests only with difficulties.
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Pulogu, Siska Irhamnawati, Kikin Hamzah Mutaqin, and Giyanto Giyanto. "Cara Preservasi Fitoplasma dari Jaringan Kacang Tanah Bergejala Sapu untuk Deteksi DNA dengan Teknik PCR." Jurnal Fitopatologi Indonesia 13, no. 2 (March 30, 2017): 43–50. http://dx.doi.org/10.14692/jfi.13.2.43.

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Yuliantini, Anne. "DETEKSI TESPONG (Oenanthe javanica) PADA BAHAN BAKU DAUN ASHITABA (Angelica keiskei) MENGGUNAKAN METODE FTIR YANG DIKOMBINASIKAN DENGAN PCA." INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL 5, no. 2 (October 30, 2020): 114–23. http://dx.doi.org/10.52447/inspj.v5i2.4230.

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Ashitaba (Angelica keiskei) merupakan salah satu tanaman yang digunakan sebagai bahan baku obat tradisional dan tespong (Oenanthe javanica) diketahui sebagai tanaman yang satu famili dengan ashitaba. Karena ketersediaan ashitaba yang sedikit ditambah dengan harganya yang relative mahal, dapat menjadi alasan ditambahkannya bahan lain dalam bahan baku ashitaba. Tujuan dari penelitian ini adalah untuk mengidentifikasi adanya tespong pada serbuk daun ashitaba menggunakan metode FTIR yang dikombinasikan dengan PCA. Penelitian ini terdiri dari 4 tahapan utama, yaitu determinasi tanaman, maserasi dengan etanol, pengukuran spectrum IR, dan analisis PCA. Hasil analisis PCA menunjukkan nilai PC1 dan PC2 berturut-turut sebesar 71 dan 22% dengan nilai eigen value lebih dari 1 dan plot PCA menggambarkan keterpisahan daerah antara ashitaba dan tespong. Dari ketiga sampel yang diproyeksikan terhadap plot PCA, terdapat sampel yang diduga mengandung tespong dan bahan campuran lain. Dari hasil penelitian ini disimpulkan bahwa metode FTIR yang dikombinasikan dengan PCA dapat menjadi metode alternative dalam mendeteksi tespong dalam bahan baku ashitaba.Kata kunci: Ashitaba; FTIR; PCA; tespong
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Jolobe, O. M. "PCR to detect M tuberculosis." Journal of Clinical Pathology 52, no. 5 (May 1, 1999): 399. http://dx.doi.org/10.1136/jcp.52.5.399.

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&NA;. "PCR can detect poor debrisoquine metabolism." Inpharma Weekly &NA;, no. 754 (September 1990): 16. http://dx.doi.org/10.2165/00128413-199007540-00041.

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Kontanis, Elias J., and Floyd A. Reed. "Evaluation of Real-Time PCR Amplification Efficiencies to Detect PCR Inhibitors." Journal of Forensic Sciences 51, no. 4 (July 2006): 795–804. http://dx.doi.org/10.1111/j.1556-4029.2006.00182.x.

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Dissertations / Theses on the topic "PCR detekce"

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Šurková, Alice. "Optimalizace izolace DNA jogurtových kultur a její detekce pomocí RT-PCR." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376848.

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The thesis has optimized DNA isolation from pure yoghurt cultures and yoghurt products. The isolated DNA was than subjected to RT-PCR analysis. In the first part of the thesis, DNA isolation from pure yoghurt cultures using a commercial kit was evaluated as more effective than isolation by phenol extraction and magnetic microparticles. To assess the quality and quantity of DNA obtained the spectrophotometric determination of concentration and purity and qPCR were used. DNA of a total of ten pure yoghurt cultures in a quality suitable for PCR was obtained using the commercial kit. In the second part of the thesis, bacterial DNA was isolated from yoghurt products using the same commercial kit with a previous sample washing by lysation solution. DNA of six yoghurt products was isolated this way. Furthermore, two packages of homemade yoghurt were mad of each product, of which DNA was isolated in the same way. DNA obtained from yoghurts was subjected to RT-PCR using six pairs of primers (V3_F a V3_R, V6_F a V6_R, V1_F a V1_R, GroHRM_F a GroHRM_R, UPF a UPR, P1V1 a P2V1) and using the pure cultures DNA as a positive controls. The results confirmed the presence of cultures declared in each yoghurt and their ability to multiply after inoculation into a new medium (milk).
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Plášková, Anna. "Stanovení autenticity potravin rostlinného původu pomocí molekulárních metod." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2020. http://www.nusl.cz/ntk/nusl-433058.

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The aim of presented diploma thesis was to determination of authenticity of fruit baby foods for early infant feeding using molecular methods. In the experimental part, isolation kit was used for isolation of plant DNA from fruits (strawberry, apricot, raspberry, apple) and from six commercial fruit products for children. Isolated DNA was characterized and verified using PCR methods with primers specific for plant rDNA (ITS2). Specific primer pairs were designed to amplify DNA for the detection of one fruit species. Primer specificity was assessed with four fruit species. A mixture of fruit puree from the two fruits was used to determine the sensitivity of the multiplex PCR assay. Six commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. The methodology of molecular detection of fruit DNA by qPCR and multiplex qPCR (duplex) includes approaches, which enable to detect two fruits (strawberry-raspberry, apricot-apple) in one reaction and thus reduces time and money requirements.
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Varga, Tomáš. "Detekce objektů na desce pracovního stolu." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-234888.

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This work describes the issue of tabletop object detection in point cloud. Point cloud is recorded with Kinect sensor. Designed solution uses algorithm RANSAC for plane detection, algorithm Euclidean clustering for segmentation and ICP algorithm for object detection. Algorithm ICP is modified and mainly it can detect rotational symetric objects and objects without any transformation against it's models. The final package is build on platform ROS. The achieved results with own dataset are good despite of the limited functionality of the detector.
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Vacek, Michal. "Detekce poznávací značky v obraze." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2009. http://www.nusl.cz/ntk/nusl-235496.

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In first part thesis contains known methods of license plate detection. Preprocessing-based methods, AdaBoost-based methods and extremal region detection methods are described.Finally, there is a described and implemented own access using local detectors to creating visual vocabulary, which is used to plate recognition. All measurements are summarized on the end.
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Kratochvíl, Jiří Jaroslav. "Detekce a vizualizace specifických rysů v mračnu bodů." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-385286.

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The point cloud is an unorganized set of points with 3D coordinates (x, y, z) which represents a real object. These point clouds are acquired by the technology called 3D scanning. This scanning technique can be done by various methods, such as LIDAR (Light Detection And Ranging) or by utilizing recently developed 3D scanners. Point clouds can be therefore used in various applications, such as mechanical or reverse engineering, rapid prototyping, biology, nuclear physics or virtual reality. Therefore in this doctoral Ph.D. thesis, I focus on feature detection and visualization in a point cloud. These features represent parts of the object that can be described by the well--known mathematical model (lines, planes, helices etc.). The points on the sharp edges are especialy problematic for commonly used methods. Therefore, I focus on detection of these problematic points. This doctoral Ph.D. thesis presents a new algorithm for precise detection of these problematic points. Visualization of these points is done by a modified curve fitting algoritm with a new weight function that leads to better results. Each of the proposed methods were tested on real data sets and compared with contemporary published methods.
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Pospíšil, Aleš. "Detekce a sledování polohy hlavy v obraze." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218928.

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Diplomová práce je zaměřena na problematiku detekce a sledování polohy hlavy v obraze jako jednu s možností jak zlepšit možnosti interakce mezi počítačem a člověkem. Hlavním přínosem diplomové práce je využití inovativních hardwarových a softwarových technologií jakými jsou Microsoft Kinect, Point Cloud Library a CImg Library. Na úvod je představeno shrnutí předchozích prací na podobné téma. Následuje charakteristika a popis databáze, která byla vytvořena pro účely diplomové práce. Vyvinutý systém pro detekci a sledování polohy hlavy je založený na akvizici 3D obrazových dat a registračním algoritmu Iterative Closest Point. V závěru diplomové práce je nabídnuto hodnocení vzniklého systému a jsou navrženy možnosti jeho budoucího zlepšení.
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Davis, Shane Brian. "A New qPCR Assay to Detect Geosmin-Producing Cyanobacteria." BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/7756.

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Taste-and-odor (T&O) compounds are frequently produced by cyanobacterial blooms in bodies of water. Geosmin, perhaps the most common T&O compound produced by these blooms, is not effectively removed by conventional water treatment processes and frequently causes the tap water to have an off flavor. Although geosmin is not harmful when ingested, it damages the consumers' confidence in the cleanliness of their water. There are treatment options for geosmin removal, but the most common methods are often not implemented until complaints are made by consumers.There has been an increasing amount of research on the use of polymerase chain reaction (PCR)-based methods that can detect the presence of the geosmin synthase gene which is responsible for the production of geosmin. If the geosmin synthase gene is found to be present in an emerging cyanobacterial bloom, water treatment facilities can prepare in advance to treat for geosmin. In this study, we developed a qPCR (quantitative polymerase chain reaction) assay that can detect the presence of the geosmin synthase gene in several species of cyanobacteria within the Anabaena genus. We tested our assay, as well as PCR assays designed by Giglio et al. (2008) and Suurnäkki et al. (2015) on extracted Anabaena flos-aquae DNA, biosynthesized Anabaena ucrainica DNA and DNA extracted from environmental samples of Deer Creek Reservoir, Strawberry Reservoir, and Utah Lake. It is important to note that the geosmin gene was not confirmed to be present in any of the environmental samples nor in the Anabaena flos-aquae DNA and our assay did not test positive on these samples. Our qPCR assay was very successful when used with the biosynthesized Anabaena ucrainica DNA. We used the results to estimate a DNA standard curve that can be used to estimate the starting concentration of the geosmin synthase gene. Because our assay was not successfully used with any extracted DNA, further testing and calibration may be necessary to produce a DNA standard curve that is representative of DNA that is extracted. Further calibration of the DNA standard curve was not done because there were no geosmin events during the course of the research.Development of PCR-based methods of detecting geosmin-producing cyanobacteria requires genetic sequencing information of the target-organisms. Thus, further development of PCR-based methods requires that the local geosmin-producers be identified and sequenced. Our assay as well as the assay designed by Moore (2019) can assist with the identification of these species by classifying their genus.
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Marko, Peter. "Detekce objektů v laserových skenech pomocí konvolučních neuronových sítí." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2021. http://www.nusl.cz/ntk/nusl-445509.

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This thesis is aimed at detection of lines of horizontal road markings from a point cloud, which was obtained using mobile laser mapping. The system works interactively in cooperation with user, which marks the beginning of the traffic line. The program gradually detects the remaining parts of the traffic line and creates its vector representation. Initially, a point cloud is projected into a horizontal plane, crating a 2D image that is segmented by a U-Net convolutional neural network. Segmentation marks one traffic line. Segmentation is converted to a polyline, which can be used in a geo-information system. During testing, the U-Net achieved a segmentation accuracy of 98.8\%, a specificity of 99.5\% and a sensitivity of 72.9\%. The estimated polyline reached an average deviation of 1.8cm.
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Nätterkvist, Ylva. "Development of a PCR method to detect HLA-B27 in ankylosing spondylitis." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-183934.

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The aim of the project was to develop a PCR method to detect HLA-B27 at the Immunology Department of St. James hospital in Dublin. The HLA-B27 gene is common among patients with ankylosing spondylitis (AS). Ninety percent of patients with AS have the HLA-B27 gene and it is therefore counted as a risk factor and could be used as part of the diagnosis. Twenty-two frozen blood samples from patients with AS or suspected AS were donated from the rheumatology department at St. James hospital. PCR is a well known and common technique, many hospital laboratories have a PCR machine and therefore PCR is a good choice for detection of the HLA-B27 gene. A multiplex PCR was developed where a PCR control, primers to the β-globin gene, was used in the same tube as the HLA-B27 primers, to secure that the PCR worked in every tube. Finally a blind test was performed to test the specificity of the PCR. The result shows that the specificity was 100%. Of all patient samples, sixteen was HLA-B27 positive and six were HLA-B27 negative. In addition, optimal conditions for the PCR and the way to extract DNA from frozen blood were successfully established. For future diagnosis, the described PCR can be used to detect the HLA-B27 gene in patients and it can be considered as a start for further development of a real-time PCR for detection of the HLA-B27.
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Hagardson, Karin. "Comparison of DNA isolation methods to detect Leishmania parasites in blood samples." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7014.

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Leishmaniasis is a disease affecting more than 12 million people worldwide. It is caused by the protozoan parasite Leishmania, which is transmitted to humans and dog hosts through bites of infected sand flies belonging to genus Phlebotomine. Several studies have shown Polymerase Chain Reaction (PCR) to be effective for the diagnosis of VL in clinical samples compared to the classical methods. The aims of this study were first to compare four different sample preparation methods for the PCR diagnosis of visceral leishmaniasis (VL) using peripheral blood samples and furthermore to find a method that is sensitive, rapid, cost benefit, simple and easy to perform. Two preparation methods were compared for the isolation of leukocytes (with Ficoll and Tris –EDTA buffer) and two DNA isolation methods (with Proteinase K and QIAgen kit). From the methods that were compared, lysis of erythrocytes with TE and the QIAgen kit seems to be the most suitable to use.

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Books on the topic "PCR detekce"

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Hajia, Massoud. Development of a multiplex PCR to detect chlamydia, mycoplasma and legionella. Manchester: University of Manchester, 1996.

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Ba-Bair, Yasser Hassan Saleh. Development of in situ PCR to detect herpes simplex virus DNA in cornea. Manchester: University of Manchester, 1996.

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Deteksi Mycobacterium tuberculosis menggunakan teknik PCR (Polymerase Chain Reaction): Laporan penelitian. Bandung: Fakultas Kedokteran, Universitas Padjadjaran, 2000.

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Isaac, Alistair M. C., and Will Bridewell. White Lies on Silver Tongues. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190652951.003.0011.

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It is easy to see that social robots will need the ability to detect and evaluate deceptive speech; otherwise they will be vulnerable to manipulation by malevolent humans. More surprisingly, we argue that effective social robots must also be able to produce deceptive speech. Many forms of technically deceptive speech perform a positive pro-social function, and the social integration of artificial agents will be possible only if they participate in this market of constructive deceit. We demonstrate that a crucial condition for detecting and producing deceptive speech is possession of a theory of mind. Furthermore, strategic reasoning about deception requires identifying a type of goal distinguished by its priority over the norms of conversation, which we call an ulterior motive. We argue that this goal is the appropriate target for ethical evaluation, not the veridicality of speech per se. Consequently, deception-capable robots are compatible with the most prominent programs to ensure that robots behave ethically.
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Lee, Amie Y., and Bonnie N. Joe. Post-Lumpectomy/Post-Radiation Breast. Edited by Christoph I. Lee, Constance D. Lehman, and Lawrence W. Bassett. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780190270261.003.0062.

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Mammography is currently the primary imaging modality for post-operative evaluation and surveillance of the conservatively treated breast. Tumor recurrence has been shown to occur at a rate of approximately 1–2% per year, and the goal of imaging surveillance is to detect recurrent and new cancers at the earliest stages while avoiding unnecessary biopsies for characteristically benign findings. The radiologist should be familiar with the expected mammographic appearance and evolution of benign post-lumpectomy/post-radiation change, while also recognizing findings suspicious for residual and recurrent disease. This chapter, appearing in the section on intervention and surgical changes, reviews the key imaging and clinical features, imaging protocols and pitfalls, and clinical recommendations for the post-lumpectomy and post-radiation breast. Topics discussed include the evolution of benign post-surgical/post-radiation findings and the detection of suspicious lesions. The primary emphasis will be on mammographic surveillance. The role of ultrasound and MRI will also be discussed.
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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Environmental DNA. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.001.0001.

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Environmental DNA (eDNA), i.e. DNA released in the environment by any living form, represents a formidable opportunity to gather high-throughput and standard information on the distribution or feeding habits of species. It has therefore great potential for applications in ecology and biodiversity management. However, this research field is fast-moving, involves different areas of expertise and currently lacks standard approaches, which calls for an up-to-date and comprehensive synthesis. Environmental DNA for biodiversity research and monitoring covers current methods based on eDNA, with a particular focus on “eDNA metabarcoding”. Intended for scientists and managers, it provides the background information to allow the design of sound experiments. It revisits all steps necessary to produce high-quality metabarcoding data such as sampling, metabarcode design, optimization of PCR and sequencing protocols, as well as analysis of large sequencing datasets. All these different steps are presented by discussing the potential and current challenges of eDNA-based approaches to infer parameters on biodiversity or ecological processes. The last chapters of this book review how DNA metabarcoding has been used so far to unravel novel patterns of diversity in space and time, to detect particular species, and to answer new ecological questions in various ecosystems and for various organisms. Environmental DNA for biodiversity research and monitoring constitutes an essential reading for all graduate students, researchers and practitioners who do not have a strong background in molecular genetics and who are willing to use eDNA approaches in ecology and biomonitoring.
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Book chapters on the topic "PCR detekce"

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McDonagh, Susan. "Ultrasensitive Quantitative PCR to Detect RNA Viruses." In PCR Protocols, 197–203. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_32.

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Moter, Sabine E., Michael D. Kramer, Markus M. Simon, Ullrich E. Schaible, R. Kinzelbach, and Reinhard Wallich. "The Polymerase Chain Reaction (PCR) to Detect Gene Sequences of Borrelia Burgdorferi, the Etiologic Agent of Lyme Disease." In PCR Topics, 206–8. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-75924-6_37.

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Bu, Fan, Li-Feng Sun, Xi-Feng Ding, Yin-Jun Miao, and Shi-Qiang Yang. "Detect and Recognize Clock Time in Sports Video." In Advances in Multimedia Information Processing - PCM 2008, 306–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-89796-5_32.

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Zheng, Qing-Fang, Ming-Ji Zhang, and Wei-Qiang Wang. "A Hybrid Approach to Detect Adult Web Images." In Advances in Multimedia Information Processing - PCM 2004, 609–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-540-30542-2_75.

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Angiulli, Fabrizio, Stefano Basta, Stefano Lodi, and Claudio Sartori. "A Distributed Approach to Detect Outliers in Very Large Data Sets." In Euro-Par 2010 - Parallel Processing, 329–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-15277-1_32.

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Nabizadeh-Ardekani, Feridon, Birger Koopmann, and Klaus Rudolph. "The Use of PCR to Detect Pseudomonas syringae pv. tomato in Planta." In Developments in Plant Pathology, 470–74. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5472-7_84.

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Delvecchio, Vito G., and Rajendra Redkar. "Use of Taqman, Light Cycler, and Confocal Microscropy to Detect Specific PCR." In Rapid Methods for Analysis of Biological Materials in the Environment, 231–37. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-015-9534-6_18.

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Levy, Scott, and Kurt B. Ferreira. "Space-Efficient Reed-Solomon Encoding to Detect and Correct Pointer Corruption." In Euro-Par 2019: Parallel Processing Workshops, 657–68. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-48340-1_50.

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Dahinden, Isabelle, Andreas Zimmermann, Marianne Liniger, and Urs Pauli. "Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products (RRS, Bt176, Bt11, Mon810, T25, Lectin, Invertase)." In Rapid Cycle Real-Time PCR — Methods and Applications, 251–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-48351-6_25.

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Irvine, Ryan A., Cindy Okitsu, and Chih-Lin Hsieh. "Q-PCR in Combination with ChIP Assays to Detect Changes in Chromatin Acetylation." In Methods in Molecular Biology, 213–23. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-316-5_16.

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Conference papers on the topic "PCR detekce"

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YANG, XIAOLIN. "LUMINESCENT PCR-ELISA ASSAY TO DETECT TB." In Proceedings of the 11th International Symposium. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811158_0090.

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Mainda, Geoffrey, Qi Webao, and Luo Manlin. "Quick PCR to detect M. tuberculosis and M. bovis in swine blood samples." In Sixth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2005. http://dx.doi.org/10.31274/safepork-180809-776.

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Davies, H., Christine E. R. Dodd, S. Tötermeyer, J. Wiseman, and Ken H. Mellits. "A rapid, sensitive enrichment PCR to detect Salmonella and ETEC infections in pigs." In Eighth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2009. http://dx.doi.org/10.31274/safepork-180809-825.

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Figallo, Cristina E., Liz Bayes, Mariana Lanata, and Veronica Etinger. "A Diagnostic Dilemma: PCR or Serology to Detect Mycoplasma Pneumoniae Pneumonia in Children." In Selection of Abstracts From NCE 2015. American Academy of Pediatrics, 2017. http://dx.doi.org/10.1542/peds.140.1_meetingabstract.39.

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Yoon, Jihye, jae jin Choi, Woohyung Choi, and Heekyung Park. "Abstract 4225: PNA mediated real-time PCR clamping assay to detect IDH1 mutations ." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4225.

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Carvajal, M. M. García, A. Rodríguez, F. Núñez, J. Delgado Perón, M. A. Asensio, and E. Bermúdez Polo. "Development of a rapid procedure of real-time PCR to detect Listeria monocytogenes in cheese." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0049.

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Nuradji, H., RM A. Adjid, and N. Nirmalasanti. "Evaluasi Tiga Prosedur Penyiapan Sampel Daging untuk Deteksi Penyakit Mulut dan Kuku dengan Uji RT-PCR." In Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Pusat Penelitian dan Pengembangan Peternakan, 2017. http://dx.doi.org/10.14334/pros.semnas.tpv-2017-p.159-166.

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Kim, Hyunsun, Jae-Jin Choi, and Heekyung Park. "Abstract 3069: One-step sensitive PNA mediated real-time PCR clamping assay to detect PIK3CA mutations." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-3069.

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"Using PCA to detect head motion from PET list mode data." In 2013 IEEE Nuclear Science Symposium and Medical Imaging Conference (2013 NSS/MIC). IEEE, 2013. http://dx.doi.org/10.1109/nssmic.2013.6829254.

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Yoon, Yeo-Sun, Donghyun Kim, Jungwon Yoon, and Soonho Choi. "Iterative Robust PCA method to detect landmines in Ground Penetrating Radar." In 2019 IEEE Radar Conference (RadarConf19). IEEE, 2019. http://dx.doi.org/10.1109/radar.2019.8835768.

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Reports on the topic "PCR detekce"

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Ham, Y., and S. Sitaraman. Development of a Safeguards Verification Method and Instrument to Detect Pin Diversion from Pressurized Water Reactor (PWR) Spent Fuel Assemblies Phase I Study. Office of Scientific and Technical Information (OSTI), December 2008. http://dx.doi.org/10.2172/946251.

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Ivanova, Iryna, and Elena Afanasieva. MODEL OF INTERACTION BETWEEN ADVERTISING, PR AND JOURNALISM. Ivan Franko National University of Lviv, February 2021. http://dx.doi.org/10.30970/vjo.2021.49.11060.

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The article is an overview of the journalism – PR – advertising relationship at the terminological, empirical-analytical and practical levels. It traces the state of the discussion of these correlations in the post-soviet media such as Ukraine. The study describes that domesticating the importance of the appropriate partnership between the three communication technologies. The thesis is that journalism, advertising and PR create a mutual connection that takes place in an atmosphere of PR and advertising permissiveness and deepens with the development of digitalization, Social network development. The present research is based on a comprehensive approach. The inductive and deductive methods are adopted to discuss theoretical materials, and the interdisciplinary research method is used to detect PR-specific features as a philosophy of a new journalism project. The interpretive approach, usually employed to analyze media text as a complex synthetic structure, was also taken into consideration. The analytical method application identified the modern means of substantiating the ideological, esthetical and informative value of brand journalism and spin doctor. The innovative character of modern media as a behavioral strategy in the advertising and PR industry consists in the fact that it is a form of creative production and behavior rather than adapting a specific communication situation. The article examines the main directions of contemporary interactions between PR, advertising and journalism as a media content creation. In this context, it is asserted that advertising, journalism and PR activities can contribute to the creation of media content. At some point, good media content is achieved not only as a result of this competition but also from the correlation between PR, advertising and journalism.
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Zilberman, Mark. Methods to Test the “Dimming Effect” Produced by a Decrease in the Number of Photons Received from Receding Light Sources. Intellectual Archive, November 2020. http://dx.doi.org/10.32370/iaj.2437.

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The hypothetical “Dimming Effect” describes the change of the number of photons arriving from a moving light source per unit of time. In non-relativistic systems, the “Dimming effect” may occur due to the growing distance of light sources moving away from the receiver. This means that due to the growing distance, the photons continuously require more time to reach the receiver, which reduces the number of received photons per time unit compared to the number of emitted photons. Understandably, the proposed “Dimming effect” must be tested (confirmed or rejected) through observations. a. This article provides the formula for the calculation of “Dimming effect” values using the redshift parameter Z widely used in astronomy. b. The “Dimming effect” can possibly be detected utilizing the orbital movement of the Earth around the Sun. In accordance to the “Dimming effect”, observers on Earth will view 1.0001 more photons per time unit emitted by stars located near the ecliptic plane in the direction of the Earth orbiting the Sun. And, in contrast, observers will view only 0.9999 photons per time unit emitted by stars located near the ecliptic plane in the direction opposite to the Earth orbiting the Sun. Calculating precise measurements of the same stars within a 6-month period can possibly detect this difference. These changes in brightness are not only for specific stars, as the change in brightness takes place for all stars near the ecliptic in the direction of the Earth’s orbit around the Sun and in the opposite direction. c. The “Dimming effect” can possibly be detected in a physics laboratory using a moving light source (or mirror) and photon counters located in the direction of travel and in the opposite direction. d. In theory, Dilation of time can also be used for testing the existence of the “Dimming effect.” However, in experiments on Earth this effect appears in only the 14th digit after the decimal point and testing does not appear to be feasible. e. Why is it important to test the “Dimming effect?” If confirmed, it would allow astronomers to adjust values of "Standard Candles" used in astronomy. Since “Standard Candles” are critical in various cosmological models, the “Dimming effect” can correct models and/or reveal and support new models. If it is proved that the “Dimming effect” does not exist, it will mean that the number of photons arriving per unit of time does not depend on the speed of the light source and observer, which is not so apparent.
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Jorgensen, Frieda, Andre Charlett, Craig Swift, Anais Painset, and Nicolae Corcionivoschi. A survey of the levels of Campylobacter spp. contamination and prevalence of selected antimicrobial resistance determinants in fresh whole UK-produced chilled chickens at retail sale (non-major retailers). Food Standards Agency, June 2021. http://dx.doi.org/10.46756/sci.fsa.xls618.

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Campylobacter spp. are the most common bacterial cause of foodborne illness in the UK, with chicken considered to be the most important vehicle for this organism. The UK Food Standards Agency (FSA) agreed with industry to reduce Campylobacter spp. contamination in raw chicken and issued a target to reduce the prevalence of the most contaminated chickens (those with more than 1000 cfu per g chicken neck skin) to below 10 % at the end of the slaughter process, initially by 2016. To help monitor progress, a series of UK-wide surveys were undertaken to determine the levels of Campylobacter spp. on whole UK-produced, fresh chicken at retail sale in the UK. The data obtained for the first four years was reported in FSA projects FS241044 (2014/15) and FS102121 (2015 to 2018). The FSA has indicated that the retail proxy target for the percentage of highly contaminated raw whole retail chickens should be less than 7% and while continued monitoring has demonstrated a sustained decline for chickens from major retailer stores, chicken on sale in other stores have yet to meet this target. This report presents results from testing chickens from non-major retailer stores (only) in a fifth survey year from 2018 to 2019. In line with previous practise, samples were collected from stores distributed throughout the UK (in proportion to the population size of each country). Testing was performed by two laboratories - a Public Health England (PHE) laboratory or the Agri-Food & Biosciences Institute (AFBI), Belfast. Enumeration of Campylobacter spp. was performed using the ISO 10272-2 standard enumeration method applied with a detection limit of 10 colony forming units (cfu) per gram (g) of neck skin. Antimicrobial resistance (AMR) to selected antimicrobials in accordance with those advised in the EU harmonised monitoring protocol was predicted from genome sequence data in Campylobacter jejuni and Campylobacter coli isolates The percentage (10.8%) of fresh, whole chicken at retail sale in stores of smaller chains (for example, Iceland, McColl’s, Budgens, Nisa, Costcutter, One Stop), independents and butchers (collectively referred to as non-major retailer stores in this report) in the UK that are highly contaminated (at more than 1000 cfu per g) with Campylobacter spp. has decreased since the previous survey year but is still higher than that found in samples from major retailers. 8 whole fresh raw chickens from non-major retailer stores were collected from August 2018 to July 2019 (n = 1009). Campylobacter spp. were detected in 55.8% of the chicken skin samples obtained from non-major retailer shops, and 10.8% of the samples had counts above 1000 cfu per g chicken skin. Comparison among production plant approval codes showed significant differences of the percentages of chicken samples with more than 1000 cfu per g, ranging from 0% to 28.1%. The percentage of samples with more than 1000 cfu of Campylobacter spp. per g was significantly higher in the period May, June and July than in the period November to April. The percentage of highly contaminated samples was significantly higher for samples taken from larger compared to smaller chickens. There was no statistical difference in the percentage of highly contaminated samples between those obtained from chicken reared with access to range (for example, free-range and organic birds) and those reared under standard regime (for example, no access to range) but the small sample size for organic and to a lesser extent free-range chickens, may have limited the ability to detect important differences should they exist. Campylobacter species was determined for isolates from 93.4% of the positive samples. C. jejuni was isolated from the majority (72.6%) of samples while C. coli was identified in 22.1% of samples. A combination of both species was found in 5.3% of samples. C. coli was more frequently isolated from samples obtained from chicken reared with access to range in comparison to those reared as standard birds. C. jejuni was less prevalent during the summer months of June, July and August compared to the remaining months of the year. Resistance to ciprofloxacin (fluoroquinolone), erythromycin (macrolide), tetracycline, (tetracyclines), gentamicin and streptomycin (aminoglycosides) was predicted from WGS data by the detection of known antimicrobial resistance determinants. Resistance to ciprofloxacin was detected in 185 (51.7%) isolates of C. jejuni and 49 (42.1%) isolates of C. coli; while 220 (61.1%) isolates of C. jejuni and 73 (62.9%) isolates of C. coli isolates were resistant to tetracycline. Three C. coli (2.6%) but none of the C. jejuni isolates harboured 23S mutations predicting reduced susceptibility to erythromycin. Multidrug resistance (MDR), defined as harbouring genetic determinants for resistance to at least three unrelated antimicrobial classes, was found in 10 (8.6%) C. coli isolates but not in any C. jejuni isolates. Co-resistance to ciprofloxacin and erythromycin was predicted in 1.7% of C. coli isolates. 9 Overall, the percentages of isolates with genetic AMR determinants found in this study were similar to those reported in the previous survey year (August 2016 to July 2017) where testing was based on phenotypic break-point testing. Multi-drug resistance was similar to that found in the previous survey years. It is recommended that trends in AMR in Campylobacter spp. isolates from retail chickens continue to be monitored to realise any increasing resistance of concern, particulary to erythromycin (macrolide). Considering that the percentage of fresh, whole chicken from non-major retailer stores in the UK that are highly contaminated (at more than 1000 cfu per g) with Campylobacter spp. continues to be above that in samples from major retailers more action including consideration of interventions such as improved biosecurity and slaughterhouse measures is needed to achieve better control of Campylobacter spp. for this section of the industry. The FSA has indicated that the retail proxy target for the percentage of highly contaminated retail chickens should be less than 7% and while continued monitoring has demonstrated a sustained decline for chickens from major retailer stores, chicken on sale in other stores have yet to meet this target.
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Holland, Darren, and Nazmina Mahmoudzadeh. Foodborne Disease Estimates for the United Kingdom in 2018. Food Standards Agency, January 2020. http://dx.doi.org/10.46756/sci.fsa.squ824.

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In February 2020 the FSA published two reports which produced new estimates of foodborne norovirus cases. These were the ‘Norovirus Attribution Study’ (NoVAS study) (O’Brien et al., 2020) and the accompanying internal FSA technical review ‘Technical Report: Review of Quantitative Risk Assessment of foodborne norovirus transmission’ (NoVAS model review), (Food Standards Agency, 2020). The NoVAS study produced a Quantitative Microbiological Risk Assessment model (QMRA) to estimate foodborne norovirus. The NoVAS model review considered the impact of using alternative assumptions and other data sources on these estimates. From these two pieces of work, a revised estimate of foodborne norovirus was produced. The FSA has therefore updated its estimates of annual foodborne disease to include these new results and also to take account of more recent data related to other pathogens. The estimates produced include: •Estimates of GP presentations and hospital admissions for foodbornenorovirus based on the new estimates of cases. The NoVAS study onlyproduced estimates for cases. •Estimates of foodborne cases, GP presentations and hospital admissions for12 other pathogens •Estimates of unattributed cases of foodborne disease •Estimates of total foodborne disease from all pathogens Previous estimates An FSA funded research project ‘The second study of infectious intestinal disease in the community’, published in 2012 and referred to as the IID2 study (Tam et al., 2012), estimated that there were 17 million cases of infectious intestinal disease (IID) in 2009. These include illness caused by all sources, not just food. Of these 17 million cases, around 40% (around 7 million) could be attributed to 13 known pathogens. These pathogens included norovirus. The remaining 60% of cases (equivalent to 10 million cases) were unattributed cases. These are cases where the causal pathogen is unknown. Reasons for this include the causal pathogen was not tested for, the test was not sensitive enough to detect the causal pathogen or the pathogen is unknown to science. A second project ‘Costed extension to the second study of infectious intestinal disease in the community’, published in 2014 and known as IID2 extension (Tam, Larose and O’Brien, 2014), estimated that there were 566,000 cases of foodborne disease per year caused by the same 13 known pathogens. Although a proportion of the unattributed cases would also be due to food, no estimate was provided for this in the IID2 extension. New estimates We estimate that there were 2.4 million cases of foodborne disease in the UK in 2018 (95% credible intervals 1.8 million to 3.1 million), with 222,000 GP presentations (95% Cred. Int. 150,000 to 322,000) and 16,400 hospital admissions (95% Cred. Int. 11,200 to 26,000). Of the estimated 2.4 million cases, 0.9 million (95% Cred. Int. 0.7 million to 1.2 million) were from the 13 known pathogens included in the IID2 extension and 1.4 million1 (95% Cred. Int. 1.0 million to 2.0 million) for unattributed cases. Norovirus was the pathogen with the largest estimate with 383,000 cases a year. However, this estimate is within the 95% credible interval for Campylobacter of 127,000 to 571,000. The pathogen with the next highest number of cases was Clostridium perfringens with 85,000 (95% Cred. Int. 32,000 to 225,000). While the methodology used in the NoVAS study does not lend itself to producing credible intervals for cases of norovirus, this does not mean that there is no uncertainty in these estimates. There were a number of parameters used in the NoVAS study which, while based on the best science currently available, were acknowledged to have uncertain values. Sensitivity analysis undertaken as part of the study showed that changes to the values of these parameters could make big differences to the overall estimates. Campylobacter was estimated to have the most GP presentations with 43,000 (95% Cred. Int. 19,000 to 76,000) followed by norovirus with 17,000 (95% Cred. Int. 11,000 to 26,000) and Clostridium perfringens with 13,000 (95% Cred. Int. 6,000 to 29,000). For hospital admissions Campylobacter was estimated to have 3,500 (95% Cred. Int. 1,400 to 7,600), followed by norovirus 2,200 (95% Cred. Int. 1,500 to 3,100) and Salmonella with 2,100 admissions (95% Cred. Int. 400 to 9,900). As many of these credible intervals overlap, any ranking needs to be undertaken with caution. While the estimates provided in this report are for 2018 the methodology described can be applied to future years.
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