To see the other types of publications on this topic, follow the link: PCR detekce.
Dissertations / Theses on the topic 'PCR detekce'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'PCR detekce.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Šurková, Alice. "Optimalizace izolace DNA jogurtových kultur a její detekce pomocí RT-PCR." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376848.
Full textAbstract:
The thesis has optimized DNA isolation from pure yoghurt cultures and yoghurt products. The isolated DNA was than subjected to RT-PCR analysis. In the first part of the thesis, DNA isolation from pure yoghurt cultures using a commercial kit was evaluated as more effective than isolation by phenol extraction and magnetic microparticles. To assess the quality and quantity of DNA obtained the spectrophotometric determination of concentration and purity and qPCR were used. DNA of a total of ten pure yoghurt cultures in a quality suitable for PCR was obtained using the commercial kit. In the second part of the thesis, bacterial DNA was isolated from yoghurt products using the same commercial kit with a previous sample washing by lysation solution. DNA of six yoghurt products was isolated this way. Furthermore, two packages of homemade yoghurt were mad of each product, of which DNA was isolated in the same way. DNA obtained from yoghurts was subjected to RT-PCR using six pairs of primers (V3_F a V3_R, V6_F a V6_R, V1_F a V1_R, GroHRM_F a GroHRM_R, UPF a UPR, P1V1 a P2V1) and using the pure cultures DNA as a positive controls. The results confirmed the presence of cultures declared in each yoghurt and their ability to multiply after inoculation into a new medium (milk).
2
Plášková, Anna. "Stanovení autenticity potravin rostlinného původu pomocí molekulárních metod." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2020. http://www.nusl.cz/ntk/nusl-433058.
Full textAbstract:
The aim of presented diploma thesis was to determination of authenticity of fruit baby foods for early infant feeding using molecular methods. In the experimental part, isolation kit was used for isolation of plant DNA from fruits (strawberry, apricot, raspberry, apple) and from six commercial fruit products for children. Isolated DNA was characterized and verified using PCR methods with primers specific for plant rDNA (ITS2). Specific primer pairs were designed to amplify DNA for the detection of one fruit species. Primer specificity was assessed with four fruit species. A mixture of fruit puree from the two fruits was used to determine the sensitivity of the multiplex PCR assay. Six commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. The methodology of molecular detection of fruit DNA by qPCR and multiplex qPCR (duplex) includes approaches, which enable to detect two fruits (strawberry-raspberry, apricot-apple) in one reaction and thus reduces time and money requirements.
3
Varga, Tomáš. "Detekce objektů na desce pracovního stolu." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-234888.
Full textAbstract:
This work describes the issue of tabletop object detection in point cloud. Point cloud is recorded with Kinect sensor. Designed solution uses algorithm RANSAC for plane detection, algorithm Euclidean clustering for segmentation and ICP algorithm for object detection. Algorithm ICP is modified and mainly it can detect rotational symetric objects and objects without any transformation against it's models. The final package is build on platform ROS. The achieved results with own dataset are good despite of the limited functionality of the detector.
4
Vacek, Michal. "Detekce poznávací značky v obraze." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2009. http://www.nusl.cz/ntk/nusl-235496.
Full textAbstract:
In first part thesis contains known methods of license plate detection. Preprocessing-based methods, AdaBoost-based methods and extremal region detection methods are described.Finally, there is a described and implemented own access using local detectors to creating visual vocabulary, which is used to plate recognition. All measurements are summarized on the end.
5
Kratochvíl, Jiří Jaroslav. "Detekce a vizualizace specifických rysů v mračnu bodů." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-385286.
Full textAbstract:
The point cloud is an unorganized set of points with 3D coordinates (x, y, z) which represents a real object. These point clouds are acquired by the technology called 3D scanning. This scanning technique can be done by various methods, such as LIDAR (Light Detection And Ranging) or by utilizing recently developed 3D scanners. Point clouds can be therefore used in various applications, such as mechanical or reverse engineering, rapid prototyping, biology, nuclear physics or virtual reality. Therefore in this doctoral Ph.D. thesis, I focus on feature detection and visualization in a point cloud. These features represent parts of the object that can be described by the well--known mathematical model (lines, planes, helices etc.). The points on the sharp edges are especialy problematic for commonly used methods. Therefore, I focus on detection of these problematic points. This doctoral Ph.D. thesis presents a new algorithm for precise detection of these problematic points. Visualization of these points is done by a modified curve fitting algoritm with a new weight function that leads to better results. Each of the proposed methods were tested on real data sets and compared with contemporary published methods.
6
Pospíšil, Aleš. "Detekce a sledování polohy hlavy v obraze." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218928.
Full textAbstract:
Diplomová práce je zaměřena na problematiku detekce a sledování polohy hlavy v obraze jako jednu s možností jak zlepšit možnosti interakce mezi počítačem a člověkem. Hlavním přínosem diplomové práce je využití inovativních hardwarových a softwarových technologií jakými jsou Microsoft Kinect, Point Cloud Library a CImg Library. Na úvod je představeno shrnutí předchozích prací na podobné téma. Následuje charakteristika a popis databáze, která byla vytvořena pro účely diplomové práce. Vyvinutý systém pro detekci a sledování polohy hlavy je založený na akvizici 3D obrazových dat a registračním algoritmu Iterative Closest Point. V závěru diplomové práce je nabídnuto hodnocení vzniklého systému a jsou navrženy možnosti jeho budoucího zlepšení.
7
Davis, Shane Brian. "A New qPCR Assay to Detect Geosmin-Producing Cyanobacteria." BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/7756.
Full textAbstract:
Taste-and-odor (T&O) compounds are frequently produced by cyanobacterial blooms in bodies of water. Geosmin, perhaps the most common T&O compound produced by these blooms, is not effectively removed by conventional water treatment processes and frequently causes the tap water to have an off flavor. Although geosmin is not harmful when ingested, it damages the consumers' confidence in the cleanliness of their water. There are treatment options for geosmin removal, but the most common methods are often not implemented until complaints are made by consumers.There has been an increasing amount of research on the use of polymerase chain reaction (PCR)-based methods that can detect the presence of the geosmin synthase gene which is responsible for the production of geosmin. If the geosmin synthase gene is found to be present in an emerging cyanobacterial bloom, water treatment facilities can prepare in advance to treat for geosmin. In this study, we developed a qPCR (quantitative polymerase chain reaction) assay that can detect the presence of the geosmin synthase gene in several species of cyanobacteria within the Anabaena genus. We tested our assay, as well as PCR assays designed by Giglio et al. (2008) and Suurnäkki et al. (2015) on extracted Anabaena flos-aquae DNA, biosynthesized Anabaena ucrainica DNA and DNA extracted from environmental samples of Deer Creek Reservoir, Strawberry Reservoir, and Utah Lake. It is important to note that the geosmin gene was not confirmed to be present in any of the environmental samples nor in the Anabaena flos-aquae DNA and our assay did not test positive on these samples. Our qPCR assay was very successful when used with the biosynthesized Anabaena ucrainica DNA. We used the results to estimate a DNA standard curve that can be used to estimate the starting concentration of the geosmin synthase gene. Because our assay was not successfully used with any extracted DNA, further testing and calibration may be necessary to produce a DNA standard curve that is representative of DNA that is extracted. Further calibration of the DNA standard curve was not done because there were no geosmin events during the course of the research.Development of PCR-based methods of detecting geosmin-producing cyanobacteria requires genetic sequencing information of the target-organisms. Thus, further development of PCR-based methods requires that the local geosmin-producers be identified and sequenced. Our assay as well as the assay designed by Moore (2019) can assist with the identification of these species by classifying their genus.
8
Marko, Peter. "Detekce objektů v laserových skenech pomocí konvolučních neuronových sítí." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2021. http://www.nusl.cz/ntk/nusl-445509.
Full textAbstract:
This thesis is aimed at detection of lines of horizontal road markings from a point cloud, which was obtained using mobile laser mapping. The system works interactively in cooperation with user, which marks the beginning of the traffic line. The program gradually detects the remaining parts of the traffic line and creates its vector representation. Initially, a point cloud is projected into a horizontal plane, crating a 2D image that is segmented by a U-Net convolutional neural network. Segmentation marks one traffic line. Segmentation is converted to a polyline, which can be used in a geo-information system. During testing, the U-Net achieved a segmentation accuracy of 98.8\%, a specificity of 99.5\% and a sensitivity of 72.9\%. The estimated polyline reached an average deviation of 1.8cm.
9
Nätterkvist, Ylva. "Development of a PCR method to detect HLA-B27 in ankylosing spondylitis." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-183934.
Full textAbstract:
The aim of the project was to develop a PCR method to detect HLA-B27 at the Immunology Department of St. James hospital in Dublin. The HLA-B27 gene is common among patients with ankylosing spondylitis (AS). Ninety percent of patients with AS have the HLA-B27 gene and it is therefore counted as a risk factor and could be used as part of the diagnosis. Twenty-two frozen blood samples from patients with AS or suspected AS were donated from the rheumatology department at St. James hospital. PCR is a well known and common technique, many hospital laboratories have a PCR machine and therefore PCR is a good choice for detection of the HLA-B27 gene. A multiplex PCR was developed where a PCR control, primers to the β-globin gene, was used in the same tube as the HLA-B27 primers, to secure that the PCR worked in every tube. Finally a blind test was performed to test the specificity of the PCR. The result shows that the specificity was 100%. Of all patient samples, sixteen was HLA-B27 positive and six were HLA-B27 negative. In addition, optimal conditions for the PCR and the way to extract DNA from frozen blood were successfully established. For future diagnosis, the described PCR can be used to detect the HLA-B27 gene in patients and it can be considered as a start for further development of a real-time PCR for detection of the HLA-B27.
10
Hagardson, Karin. "Comparison of DNA isolation methods to detect Leishmania parasites in blood samples." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7014.
Full textAbstract:
Leishmaniasis is a disease affecting more than 12 million people worldwide. It is caused by the protozoan parasite Leishmania, which is transmitted to humans and dog hosts through bites of infected sand flies belonging to genus Phlebotomine. Several studies have shown Polymerase Chain Reaction (PCR) to be effective for the diagnosis of VL in clinical samples compared to the classical methods. The aims of this study were first to compare four different sample preparation methods for the PCR diagnosis of visceral leishmaniasis (VL) using peripheral blood samples and furthermore to find a method that is sensitive, rapid, cost benefit, simple and easy to perform. Two preparation methods were compared for the isolation of leukocytes (with Ficoll and Tris –EDTA buffer) and two DNA isolation methods (with Proteinase K and QIAgen kit). From the methods that were compared, lysis of erythrocytes with TE and the QIAgen kit seems to be the most suitable to use.
11
Chevalier, Dominique. "Contribution a l'etude de la congelation par detente haute pression." Nantes, 2000. http://www.theses.fr/2000NANT2061.
Full textAbstract:
Ce travail concerne l'etude du procede de congelation par detente haute pression (cdhp). Ce procede consiste a refroidir sous pression a temperature negative un aliment sans provoquer la transition de phase liquide-solide. Une detente quasi-adiabatique est alors realisee entrainant une surfusion dans l'ensemble du materiau suivi d'une nucleation homogene et rapide. L'objectif general de ce travail visait a etudier l'effet de la cdhp sur des produits biologiques. Dans ce but, ce procede a ete compare a des procedes classiques de congelation pour des produits de complexite croissante (gel modele de gelatine, produits de la mer). Dans le cas des gels cylindriques de gelatine (2% w/w), la congelation par detente haute pression a permis d'obtenir une reduction significative de la taille des cristaux de glace, cette taille etant sensiblement constante quelque soit la position dans le produit. Le taux de nucleation de cristaux de glace, qui etait fonction du niveau de pression, a ete reliee par une loi exponentielle a la surfusion produite dans le produit au moment de la detente. Une amelioration de la microstructure des produits de la mer a ete observee pour le procede de cdhp. En particulier, la section des cristaux de glace lors de la cdhp de filets de turbot (140 mpa, 14\c) a ete reduite par un facteur dix comparee a une congelation par air pulse. Ceci est associe a une reduction des pertes par exsudation mais aussi a des modifications de texture. La derniere partie de cette etude porte sur la realisation d'un calorimetre differentiel haute pression. Ce calorimetre, qui fonctionne de maniere isotherme, a ete valide a partir du changement d'etat eau-glace et a ete utilise pour mesure la chaleur latente de gels de gelatine.
12
Kazan, Lutfallah. "Naissance et developpement du milieu diphasique par detente dans un canal." Paris 6, 1988. http://www.theses.fr/1988PA066324.
Full textAbstract:
Description de l'autovaporisation d'un liquide en ecoulement par detente dans un canal. L'etude est basee sur des resultats experimentaux obtenus par diffusion de brillouin, anemometrie doppler, diffusion de lumiere dans la direction avant et la technique des cavites hyperfrequences
13
Rust, Annette. "Development and evaluation of a PCR protocol to detect Escherichia coli in drinking water samples /." Zürich : ETH, 2007. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17499.
Full text
14
Sousa, Tarcisio Tom?s Cabral de. "Detec??o de endobact?ria e morfologia do sistema digest?rio de Thaumastocoris peregrinus." UFVJM, 2016. http://acervo.ufvjm.edu.br/jspui/handle/1/1367.
Full textAbstract:
Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-06-12T18:57:15Z
No. of bitstreams: 2
tarcisio_tomas_cabral_sousa.pdf: 1757108 bytes, checksum: 835c7914569f767d1b2d01ecdb785268 (MD5)
license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-06-14T19:16:53Z (GMT) No. of bitstreams: 2 tarcisio_tomas_cabral_sousa.pdf: 1757108 bytes, checksum: 835c7914569f767d1b2d01ecdb785268 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)
Made available in DSpace on 2017-06-14T19:16:53Z (GMT). No. of bitstreams: 2 tarcisio_tomas_cabral_sousa.pdf: 1757108 bytes, checksum: 835c7914569f767d1b2d01ecdb785268 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)
Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG)
APERAM BioEnergia
VERACEL
GERDAU
A produ??o de eucalipto vem sofrendo grandes preju?zos com o ataque de uma nova praga, o Thaumastocoris peregrinus, seu nome popular ? percevejo bronzeado devido seu h?bito alimentar, succivoro. Essa alimenta??o causa danos diretos ?s ?rvores, deixando as folhas com aspecto bronzeado, diminuindo a fotoss?ntese, chegando a secar e cair, o que pode culminar com a morte da planta. Poucos s?o os estudos relacionados a essa praga, n?o existindo um controle eficaz. Com o intuito de conhecer sobre a morfologia do sistema digest?rio, bem como a intera??o deste com micro-organismos e visando fornecer informa??es ao controle dessa praga, foram realizadas a histologia do sistema digest?rio e sequenciamento de fragmentos de DNA obtidos de micro-organismos internos ao inseto. A histologia permitiu observar que o percevejo n?o tem os cecos g?stricos, parte do intestino m?dio que armazena micro-organismos que auxiliam na digest?o dos alimentos e que ? possuidor de um par de gl?ndulas salivares que s?o compostas de dois l?bulos cada. As an?lises moleculares possibilitaram verificar que, possivelmente, existem micro-organismos presentes no sistema digest?rio vivendo em associa??o com o mesmo.
Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016.
The Eucalyptus production has suffered great losses from the attack of a new pest, the Thaumastocoris peregrinus, popularly known as bronze bug to cause silvering on eucalyptus leaves. This symptom occurs because of your eating habits, because it sucks the sap of its host. This feeding causes direct damage to trees, because its leaves stay with tanned look, reducing photosynthesis, reaching dry and fall off, which can lead to the death of the plant. There are few studies relating to this pest, with no effective control. With intuited to know about the digestive morphology and the interaction of this with micro-organisms, targeting subsidies to the control of this pest were performed histology of the digestive system and sequencing of DNA fragments obtained from internal micro-organisms to the insect. The histology allowed to observe that the bronze bug does not have the gastric cecum of the midgut that stores micro-organism to assist in digestion of food, and their digestive system is divided into three parts, foregut, middle and posterior, which is possessor of a pair of salivary glands that are composed of two lobes each. Molecular analysis enabled us to verify that, possibly, there are micro-organisms present in the digestive system living in association with the same.
15
Charvát, Jaroslav. "Ovládání počítače pomocí gest." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2011. http://www.nusl.cz/ntk/nusl-236922.
Full textAbstract:
Master's thesis "Human-Machine Interface Based on Gestures" depicts the theoretical background of the computer vision and gesture recognition. It describes more in detail different methods that were used to create the application. Practical part of this thesis consists of the description of the developed program and its functionality. Using this application, user should be able to control computer by gestures of both right and left hands and also his head. The program is primarily based on the skin detection that is followed by the recognition of palms and head gestures. There were used two essential methods for these actions, AdaBoost and PCA.
16
Peyre, Julia. "Learning to detect visual relations." Thesis, Paris Sciences et Lettres (ComUE), 2019. http://www.theses.fr/2019PSLEE016.
Full textAbstract:
Nous étudions le problème de détection de relations visuelles de la forme (sujet, prédicat, objet) dans les images, qui sont des entités intermédiaires entre les objets et les scènes visuelles complexes. Cette thèse s’attaque à deux défis majeurs : (1) le problème d’annotations coûteuses pour l’entrainement de modèles fortement supervisés, (2) la variation d’apparence visuelle des relations. Nous proposons un premier modèle de détection de relations visuelles faiblement supervisé, n’utilisant que des annotations au niveau de l’image, qui, étant donné des détecteurs d’objets pré-entrainés, atteint une précision proche de celle de modèles fortement supervisés. Notre second modèle combine des représentations compositionnelles (sujet, objet, prédicat) et holistiques (triplet) afin de mieux modéliser les variations d’apparence visuelle et propose un module de raisonnement par analogie pour généraliser à de nouveaux triplets. Nous validons expérimentalement le bénéfice apporté par chacune de ces composantes sur des bases de données réellesIn this thesis, we study the problem of detection of visual relations of the form (subject, predicate, object) in images, which are intermediate level semantic units between objects and complex scenes. Our work addresses two main challenges in visual relation detection: (1) the difficulty of obtaining box-level annotations to train fully-supervised models, (2) the variability of appearance of visual relations. We first propose a weakly-supervised approach which, given pre-trained object detectors, enables us to learn relation detectors using image-level labels only, maintaining a performance close to fully-supervised models. Second, we propose a model that combines different granularities of embeddings (for subject, object, predicate and triplet) to better model appearance variation and introduce an analogical reasoning module to generalize to unseen triplets. Experimental results demonstrate the improvement of our hybrid model over a purely compositional model and validate the benefits of our transfer by analogy to retrieve unseen triplets
17
Talsma, Alex Jeanne. "Development of a Confirmatory PCR Assay to Detect Onchocerca volvulus in Pools of Vector Black Flies." Scholar Commons, 2013. http://scholarcommons.usf.edu/etd/4952.
Full textAbstract:
Onchocerciasis, or river blindness, has historically represented one of the significant neglected tropical diseases on the planet in terms of socio-economic impact. The discovery that ivermectin was a safe and effective treatment for onchocerciasis, together with the decision of the manufacturer to donate the drug for the treatment of this disease became the basis for several large international programs to control and eventually eliminate the infection. These programs have managed to virtually eliminate transmission of the parasite causing Onchocerca volvulus from many foci in Africa and the Americas. Verifying that transmission has been halted requires sensitive and specific assays to detect the presence of the parasite. The gold standard to accomplish this has been to employ a PCR assay targeting a specific repeated sequence family encoded in the genome of O. volvulus to screen for the presence of the parasite in pools of vector black flies. While this assay is highly sensitive, obtaining the high specificity required to document an absence of transmission requires an independent confirmatory assay. To meet this need, an independent PCR assay targeting the cytochrome B (cytB) gene of the O. volvulus mitochondrion was developed. This assay could detect O. volvulus mitochondrial DNA purified by magnetic bead capture using the primers for the cytB gene and from the nuclear encoded repeated sequence DNA targeted in the primary assay. These preliminary data suggest that the mitochondrial PCR assay may be employed as a confirmatory assay to detect O. volvulus in pools of vector flies.
18
Lepoutre, Alexandre. "Détection et poursuite en contexte Track-Before-Detect par filtrage particulaire." Thesis, Rennes 1, 2016. http://www.theses.fr/2016REN1S101/document.
Full textAbstract:
Cette thèse s'intéresse à l'étude et au développement de méthodes de pistage mono et multicible en contexte Track-Before-Detect (TBD) par filtrage particulaire. Contrairement à l'approche classique qui effectue un seuillage préalable sur les données avant le pistage, l'approche TBD considère directement les données brutes afin de réaliser conjointement la détection et le pistage des différentes cibles. Il existe plusieurs solutions à ce problème, néanmoins cette thèse se restreint au cadre bayésien des Modèles de Markov Cachés pour lesquels le problème TBD peut être résolu à l'aide d'approximations particulaires. Dans un premier temps, nous nous intéressons à des méthodes particulaires monocibles existantes pour lesquels nous proposons différentes lois instrumentales permettant l'amélioration des performances en détection et estimation. Puis nous proposons une approche alternative du problème monocible fondée sur les temps d'apparition et de disparition de la cible; cette approche permet notamment un gain significatif au niveau du temps de calcul. Dans un second temps, nous nous intéressons au calcul de la vraisemblance en TBD -- nécessaire au bon fonctionnement des filtres particulaires -- rendu difficile par la présence des paramètres d'amplitudes des cibles qui sont inconnus et fluctuants au cours du temps. En particulier, nous étendons les travaux de Rutten et al. pour le calcul de la vraisemblance au modèle de fluctuations Swerling et au cas multicible. Enfin, nous traitons le problème multicible en contexte TBD. Nous montrons qu'en tenant compte de la structure particulière de la vraisemblance quand les cibles sont éloignées, il est possible de développer une solution multicible permettant d'utiliser, dans cette situation, un seule filtre par cible. Nous développons également un filtre TBD multicible complet permettant l'apparition et la disparition des cibles ainsi que les croisementsThis thesis deals with the study and the development of mono and multitarget tracking methods in a Track-Before-Detect (TBD) context with particle filters. Contrary to the classic approach that performs before the tracking stage a pre-detection and extraction step, the TBD approach directly works on raw data in order to jointly perform detection and tracking. Several solutions to this problem exist, however this thesis is restricted to the particular Hidden Markov Models considered in the Bayesian framework for which the TBD problem can be solved using particle filter approximations.Initially, we consider existing monotarget particle solutions and we propose several instrumental densities that allow to improve the performance both in detection and in estimation. Then, we propose an alternative approach of the monotarget TBD problem based on the target appearance and disappearance times. This new approach, in particular, allows to gain in terms of computational resources. Secondly, we investigate the calculation of the measurement likelihood in a TBD context -- necessary for the derivation of the particle filters -- that is difficult due to the presence of the target amplitude parameters that are unknown and fluctuate over time. In particular, we extend the work of Rutten et al. for the likelihood calculation to several Swerling models and to the multitarget case. Lastly, we consider the multitarget TBD problem. By taking advantage of the specific structure of the likelihood when targets are far apart from each other, we show that it is possible to develop a particle solution that considers only a particle filter per target. Moreover, we develop a whole multitarget TBD solution able to manage the target appearances and disappearances and also the crossing between targets
19
Maňkoš, Richard. "Rozpoznávání obličeje." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-241994.
Full textAbstract:
This diploma thesis deals with face recognition in digital pictures. The first part describes biometry and, shortly, characterizes biometrical methods which are the most oftenly used. In the second part is described the approach of face recognition in a picture. Specifically, it is described the method for face detection - Viola-Jones and method for face recognition - PCA, which will be implemented in Matlab. The last part, which is practical, describes the scheme for video-sequence recording, implementation of the PCA method in Matlab and discussion of the achieved results.
20
Botsaris, George. "Development and evaluation of a rapid phage-PCR assay to detect mycobacterium avium subsp. paratuberculosis in dairy products." Thesis, University of Nottingham, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.537656.
Full text
21
Kaminska, Monika. "New activity-based probes to detect matrix metalloproteases." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS538/document.
Full textAbstract:
Les Métallo Protéases Matricielles (MMP) en tant qu'endopeptidases à zinc ont une large gamme de fonctions biologiques allant du remodelage tissulaire à la modulation de la réponse cellulaire. Une modification de leur activité protéolytique est souvent associée à de nombreux désordres biologiques. In vivo, ces protéases sont soumises à de nombreuses modifications post-traductionnelles. Elles sont sécrétées sous formes latentes à l'extérieur des cellules pour être ensuite transformées en forme fonctionnelles. Ces dernières sont ensuite inhibées par des inhibiteurs endogènes. En raison de leur sécrétion dans l’espace extra cellulaire, les MMP sous formes actives ont longtemps été considérées comme de simples ciseaux moléculaires capable de dégrader uniquement la matrice extracellulaire. Cependant, le remodelage tissulaire ne constitue pas la fonction unique et encore moins la fonction principale de ces enzymes. Elles peuvent en effet cliver une grande variété de substrats non matriciels et à ce titre sont impliquées dans la progression tumorale, l'immunité et l'inflammation. Pour ajouter une complexité supplémentaire à la biologie des MMP, il a été récemment montré que certaines MMP ont une localisation intracellulaire associée à des fonctions non protéolytiques. Ces observations, mais aussi celles montrant que ces protease participent à la progression de la maladie alors que d'autres ont une fonction protectrice, soulignent la nécessité de mieux documenter leur activation spatiale et temporelle dans divers contextes biologiques.Le profilage protéique basé sur l'activité vise à analyser l'état fonctionnel des protéines dans des échantillons biologiques complexes. À cette fin, des sondes basées sur l'activité (ABP), qui réagissent avec les enzymes en s’appuyant sur leur mécanisme catalytique, ont été développées pour la détection d’enzymes sous formes actives, notamment dans le cas des protéases à sérine et à cystéine. Une sonde basée sur l’activité (ABP) est classiquement composée : i) d’un groupement réactif conduisant à la modification covalente de résidus au sein du site actif de l’enzyme, ii) d’un motif de liaison imposant la sélectivité au groupement réactif et iii) d’un groupement rapporteur permettant la détection des enzymes ciblées. Cette approche ne s’applique toutefois pas aux MMP, pour lesquelles il n’existe pas de résidus nucléophiles conservés au sein du site actif. À cet égard, tous les ABP ciblant les MMP comportent un groupement photo activable qui, sous irradiation UV, favorise la formation du complexe covalent. De telles sondes photo sensibles ont permis de détecter les MMP sous leurs formes actives dans des tissus et des fluides, mais pas chez les animaux vivants au sein desquels l’étape de photo-activation ne peut être réalisé.Dans ce contexte, en nous appuyant sur un contexte structural favorable et en exploitant la chimie de l'acyl imidazole (LDAI) dirigée par un ligand, nous avons identifié une nouvelle série de sondes capables de modifier de manière covalente les MMP sans recourir à la photo-activation. Nous avons ainsi validé la capacité de ces sondes à marquer de manière sélective et efficace la MMP12 humaine in vitro et dans des protéomes complexes. Dans ce dernier cas, jusqu’à 50ng de hMMP12 correspondant à 0,05% du protéome total peuvent être détectés. Nous avons également déterminé l'identité de l’unique résidu modifié de façon covalente au sein du site actif de la hMMP-12 et vérifié que cette modification avait peu d'impact sur l’activité protéolytique de cette dernière. Nous avons démontré que cette approche permettait de détecter des MMP endogènes. Enfin, nous avons étendu cette stratégie de marquage à un panel plus large de MMP.En développant la première stratégie de marquage des formes actives de MMP «sans photo-activation», il semble maintenant possible d’envisager la détection de ces enzymes à la fois dans les protéomes complexes et in vivoMatrix MetalloProteases (MMPs) as zinc endopeptidases have a wide range of biological functions, and changes in their proteolytic activity underlie many biological disorders. Since their proteolytic activity has to be tightly controlled to prevent tissue destruction, theses proteases are subjected to numerous posttranslational modifications in vivo. They are secreted under latent forms outside of the cells, and are subsequently processed into their functional form that can be further inhibited by endogenous inhibitors. Due to their delineated area of activation, MMP active forms have long been considered for their unique ability to degrade extracellular substrates. However, turnover and breakdown of the extracellular matrix are neither the sole nor the main function of MMPs. These enzymes can indeed process a wide variety of non-matrix substrates and are involved in the regulation of multiple aspects of tumor progression, immunity and inflammation. To add further complexity to MMPs biology, some members within the family were recently reported to have intracellular localization associated to non-proteolytic functions. These observations but also those evidencing that some MMPs participate in disease progression while others have a protective function, stress the need to better document their spatial and temporal activation in various biological contexts.Activity-based protein profiling (ABPP) aims to analyze the functional state of proteins within complex biological samples. To this purpose, activity-based probes (ABPs) that react with enzymes in a mechanism-based manner have been successfully developed for the profiling of several enzymes including serine and cysteine proteases. A typical Activity-Based probe (ABP) is composed of i) a reactive warhead, which reacts in a covalent manner with enzyme active site residues, ii) a targeting moiety that imposes selectivity upon the reactive group and iii) a detectable group for subsequent analyses. This approach is not applicable to MMPs, which lack a targetable nucleophile involved in the catalysis. In this respect, all ABPs directed to MMPs are affinity-based probes (AfBPs) containing within their structure a photo cross-linking group that promotes the formation of a covalent complex upon UV-irradiation. Such photoactivatable probes have been successfully developed for the detection of MMPs under their active forms in fluids and tissue extracts, but not in living animals where the photo-activation step is not feasible.By relying on a favorable structural context and by exploiting the ligand-directed acyl imidazole (LDAI) chemistry, we have identified a novel series of AfBPs capable of covalently modifying matrix metalloproteases without making use of photo-activation. These active-site-directed probes whose structure was derived from that of a MMP12 selective inhibitor harbored a reactive acyl imidazole in their P3' position. They demonstrated their labelling specificity in vitro by covalently modifying a single Lysine residue within the MMP-12 S3' region. We also showed that these probes only targeted functional states of hMMP-12 and spared forms whose active site was occluded either by a synthetic or a natural inhibitor. We have validated the ability of these chemical probes to efficiently label human MMP12 in complex proteomes. In this case, down to 50 ng of hMMP12 corresponding to 0.05% of the whole proteome can be labelled and detected by in-gel fluorescence analysis. We demonstrated that this approach also allowed detecting endogenous MMPs secreted by stimulated-macrophages. In addition, by modifying the nature of the targeting moiety, we have extended this affinity-labeling approach to six other MMPs.By developing the first “photo activation-free” strategy to covalently modify active forms of MMPs, the unresolved proteomic profiling of native MMPs should be now accessible both in complex proteomes and in preclinical model in which MMPs are potential relevant targets
22
Freyberg, Stefanie. "The international dimension of the SPD and the PCI : Europe, the Cold War and Detente." Thesis, Queen Mary, University of London, 2009. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1632.
Full textAbstract:
This thesis compares the foreign policy course undertaken by the German Social Democratic Party (SPD) and the Italian Communist Party (PC I) in the 1960s and 1970s. The thesis analyses where the foreign policy of the two parties converges and diverges, especially with regard to international detente, security policies, the Eastern bloc and European integration. The choice of a broad time frame is justified by the diverse timing and junctures of the SPD's and the PCI's revisionist course. Departing from the assumption that both SPD and PCI were characterised by their national roots and ambitions, the thesis seeks to arrive at party overlapping trends and conclusions as to how political parties address and overcome national and international constraints in spite of ideological divergences. One aim of the comparison is to examine policy revisionism and analyse its ongIns and motivations. Revisionism has its origins in a number of interrelated factors which are often not mutually exclusive. Policy shifts are every so often caused by ideological reconsiderations or a rethinking of, and adaptation to political, economic and social circumstances. The comparative method allows one to make general assumptions and draw parallels about the origins of revisionism, and relate them, where possible, to wider sections of the Western European Left. This process which occurred in the wider context of de-ideologisation was not distinctive to Italian communism or German social democracy but can be observed by examining Western European parties of the Left in general.
23
Matuszek, Martin. "Měření pulzu z videa." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2014. http://www.nusl.cz/ntk/nusl-236098.
Full textAbstract:
The aim of the Master's thesis was to study contemporary methods for human pulse detection from standard video and suggest a method, which can be used to detect the pulse. Approaches of detecting miniature changes between frames of a video are presented. Position changes of the feature points or changes in colour of some part of an image are detected. It capitalize on the fact that those changes are caused by the pulse of blood. The method for color changes magnification is selected as a base for pulse detector. Face regions of interest are analyzed to detect frequency of changes of intensity between frames. 1D signal is gained and its analysis leads to heart rate. Approach to create heat map of frequency changes is also presented.
24
Cavalcante, Gustavo Henrique Oliveira. "Estudo da variabilidade gen?tica do papilomav?rus humano e determina??o de alvos moleculares para detec??o e tipagem." PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, 2018. https://repositorio.ufrn.br/jspui/handle/123456789/24993.
Full textAbstract:
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-04-02T15:02:43Z
No. of bitstreams: 1
GustavoHenriqueOliveiraCavalcante_DISSERT.pdf: 14332647 bytes, checksum: 07f54cbd966599c411e90b07dbb86ba8 (MD5)Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-04-05T13:57:34Z (GMT) No. of bitstreams: 1 GustavoHenriqueOliveiraCavalcante_DISSERT.pdf: 14332647 bytes, checksum: 07f54cbd966599c411e90b07dbb86ba8 (MD5)
Made available in DSpace on 2018-04-05T13:57:34Z (GMT). No. of bitstreams: 1 GustavoHenriqueOliveiraCavalcante_DISSERT.pdf: 14332647 bytes, checksum: 07f54cbd966599c411e90b07dbb86ba8 (MD5) Previous issue date: 2018-02-23
O papilomav?rus humano (HPV) ? um pequeno v?rus de DNA de dupla fita circular, caracterizado como um dos mais comuns agentes sexualmente transmiss?veis no mundo, cuja detec??o e genotipagem acuradas s? s?o poss?veis por meio de t?cnicas de biologia molecular. Diferentes propriedades biol?gicas t?m sido reportadas dentre os mais de 170 tipos j? caracterizados, de maneira que um grupo particular de HPVs est? fortemente relacionado a infec??es persistentes, les?es intraepiteliais de diferentes graus e progress?o para c?nceres tais como cervical, anal, vulvar, vaginal, orofar?ngeo e de p?nis. No presente trabalho foram realizadas an?lises de variabilidade gen?tica e evolu??o molecular nos genomas dos principais HPVs de import?ncia cl?nica. Reconstru??es filogen?ticas e an?lises dos perfis de assinatura gen?tica nos genomas de cada gen?tipo sugeriram a presen?a de subgrupos de HPVs definidos por diferen?as nas sequ?ncias dos genes E1, E6, L1 e L2. Testes de evolu??o em n?vel de DNA revelaram uma atua??o mais forte da sele??o natural em c?dons espec?ficos, mais frequentemente nos genes E1, E2, L1 e L2. A partir dos dados obtidos nas an?lises de variabilidade, foi desenhado um novo conjunto de primers para a detec??o e genotipagem dos HPVs de import?ncia cl?nica por meio da t?cnica de rea??o em cadeia da polimerase (PCR). O gene E1 foi escolhido como alvo molecular devido a presen?a de uma regi?o conservada com tamanho vari?vel entre gen?tipos. O sistema proposto teve sua efici?ncia avaliada in vitro e foi comparado ao protocolo de PCR mais utilizado para detec??o do HPV em amostras cl?nicas. Utilizando a amplifica??o de ?cido nucleico de forma semianinhada (seminested), o sistema proposto foi capaz de detectar com boa sensibilidade alguns dos principais HPVs de alto risco oncog?nico e mostrou melhor especificidade em rela??o aos primers gen?ricos GP5+/6+, mesmo aplicando uma temperatura de anelamento consideravelmente maior. A an?lise do tamanho dos fragmentos amplificados usando a separa??o por eletroforese em gel de agarose pode favorecer a identifica??o do tipo de HPV presente nas amostras, permitindo a discrimina??o entre aqueles mais prevalentes na popula??o e a redu??o do tempo e do custo necess?rios para a identifica??o do agente. Opcionalmente, a separa??o dos produtos em matrizes de alta resolu??o e o sequenciamento direto podem ser usados para a tipagem, possibilitando a identifica??o de uma ampla variedade de gen?tipos de HPV descritos.
Human papillomavirus (HPV) is a small circular double-stranded DNA virus, characterized as one of the most common sexually transmitted agents in the world, whose accurate detection and genotyping is only possible through molecular biology techniques. Different biological properties have been reported among the more than 170 types already characterized, so that a particular group of HPVs is strongly related to persistent infections, intraepithelial lesions of different degrees and progression to cancers such as cervical, anal, vulvar, vaginal, oropharyngeal and penis. In the present work, analyzes of genetic variability and molecular evolution were performed in the genomes of the main clinically important HPVs. Phylogenetic reconstructions and analyzes of genetic signature profiles in the genomes of each genotype suggested the presence of subgroups of HPVs defined by differences in the E1, E6, L1 and L2 gene sequences. Evolution tests at DNA level have shown a stronger acting of natural selection at specific codons, more often in the E1, E2, L1 and L2 genes. From the data obtained in the analyzes of variability, a new set of primers was designed for the detection and genotyping of HPVs of clinical importance by polymerase chain reaction (PCR) technique. E1 gene was chosen as the molecular target due to the presence of a conserved region of variable size among genotypes. The proposed system had its efficiency evaluated in vitro and was compared to the most used PCR protocol for HPV detection in clinical samples. Using the seminested nucleic acid amplification, the proposed system was able to detect some of the major oncogenic HPVs with good sensitivity and showed a better specificity than the generic primers GP5+/6+, even applying a considerably higher annealing temperature. The analysis of the size of the amplified fragments using agarose gel electrophoresis may favor the identification of the HPV type present in the samples, allowing the discrimination between those more prevalent in the population and the reduction of the time and cost necessary for the identification of the agent. Optionally, the separation of products into high resolution matrices and direct sequencing can be used for typing, enabling the identification of a wide variety of HPV genotypes described.
25
Keeley, Ryan F. "Design and Implementation of Degenerate qPCR/qRT-PCR Primers to Detect Microbial Nitrogen Metabolism in Wastewater and Wastewater-Related Samples." Scholar Commons, 2019. https://scholarcommons.usf.edu/etd/7826.
Full textAbstract:
Nitrogen cycling processes can be tracked using quantitative Polymerase Chain Reaction (qPCR) to determine the presence and qReverse Transcriptase-PCR (qRT-PCR) to determine expression of key genes, or ‘biological markers’, for nitrogen metabolism. Nitrification is catalyzed in part, by two enzymes: ammonia monooxygenase (AMO; NH3 NH2OH) and nitrite oxidoreductase (NXR; NO2- NO3-). For denitrification, four enzymes act sequentially: nitrate reductase (NAR/NAP; NO3- NO2-), nitrite reductase (NIR; NO2- NO), nitric oxide reductase (NOR; NO N2O), and nitrous oxide reductase (NOS; N2O N2). A principle of wastewater treatment (WWT) is to remove excess nitrogen by taking advantage of natural nitrogen cycling or biological nitrogen removal (BNR). This process involves using microorganisms to bring influent ammonia through nitrification and denitrification to release nitrogen gas, which does not contribute to eutrophication. A novel shortcut nitrogen removal configuration could increase nitrogen removal efficiency by promoting nitritation/denitritation, reducing the classic nitrogen cycle by removing the redundant oxidation/reduction step to nitrate (NO3-). Here, three nitrogen transformations were used to track the three main phases in the nitrogen cycle; ammonia monooxygenase for nitrification, nitrite oxidoreductase for shortcut, and nitrous oxide reductase for denitrification. Primers for qPCR and qRT-PCR were designed to capture as much sequence diversity as possible for each step. Genes from bacteria known to perform the nitrogen transformations of interest (amoA, nxrB, nosZ) were used to BLAST-query the Integrated Microbial Genomes & Microbiomes database (img.jgi.doe.gov) to find homologs from organisms commonly found in WWT. These sequences were then aligned to find regions sufficiently conserved for primer design. These PCR primers were tested against standards for each gene and used to track nitrogen transformation potential and expression in a novel lab-scale algal photo-sequencing batch reactor which promotes shortcut nitrogen removal from wastewater across three solids retention times (SRT, or mean cell residence time); 5, 10 and 15 days. SRT 15 had the greatest total nitrogen removal with nitritation and denitritation observed. Nitrate was not detected in the first cycle and shortcut nitrogen removal was supported by low levels of nxrB genes and transcripts. Simultaneous nitrification/denitrification was supported by elevated concentrations of nosZ during the light period and less nitrite produced than ammonium consumed. Nitritation was predominantly performed by Betaproteobacteria amoA and nitrous oxide reduction was predominantly from nosZ group I (Proteobacteria-type).
26
Diefenderfer, Brian K. "Development and Testing of a Capacitor Probe to Detect Deterioration in Portland Cement Concrete." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/35397.
Full textAbstract:
Portland cement concrete (PCC) structures deteriorate with age and need to be maintained or replaced. Early detection of deterioration in PCC (e.g., alkali-silica reaction, freeze/thaw damage or chloride presence) can lead to significant reductions in maintenance costs. Portland cement concrete can be nondestructively evaluated by electrically characterizing its complex dielectric constant in a laboratory setting. A parallel-plate capacitor operating in the frequency range of 0.1 to 40.1 MHz was developed at Virginia Tech for this purpose. While useful in research, this approach is not practical for field implementation. In this study, a capacitor probe was designed and fabricated to determine the in-situ dielectric properties of PCC over a frequency range of 2.0 to 20.0 MHz. It is modeled after the parallel-plate capacitor in that it consists of two conducting plates with a known separation. The conducting plates are flexible, which allows them to conform to different geometric shapes. Prior to PCC testing, measurements were conducted to determine the validity of such a system by testing specimens possessing known dielectric properties (Teflon). Portland cement concrete specimens were cast (of sufficient size to prevent edge diffraction of the electromagnetic waves) having two different air contents, two void thicknesses, and two void depths (from the specimen's surface). Two specimens were cast for each parameter and their results were averaged. The dielectric properties over curing time were measured for all specimens, using the capacitor probe and the parallel-plate capacitor. The capacitor probe showed a decrease in dielectric constant with increasing curing time and/or air content. In addition to measuring dielectric properties accurately and monitoring the curing process, the capacitor probe was also found to detect the presence and relative depth of air voids, however, determining air void thickness was difficult.Master of Science
27
SILVA, F?bio Jorge Moreira da. "Detec??o de Anaplasma marginale por pesquisa de IgG e PCR em um rebanho bovino da Baixada Fluminense." Universidade Federal Rural do Rio de Janeiro, 2012. https://tede.ufrrj.br/jspui/handle/jspui/1629.
Full textAbstract:
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-11T19:12:48Z
No. of bitstreams: 1
2012 - F?bio Jorge Moreira da Silva.pdf: 1417566 bytes, checksum: a168314142bf607a64227972ff4c85e8 (MD5)Made available in DSpace on 2017-05-11T19:12:48Z (GMT). No. of bitstreams: 1 2012 - F?bio Jorge Moreira da Silva.pdf: 1417566 bytes, checksum: a168314142bf607a64227972ff4c85e8 (MD5) Previous issue date: 2012-06-06
CNPq
Activities in high productivity systems to dairy cattle contribute to imbalance health and disease, and increase the possibility of illness compatible with the infestation and infections transmitted by arthropods vectors. The objectives were to evaluate the prevalence and the seroepidemiological condition for Anaplasma marginale in 41calves from birth to completed 180 days. The animals belonged to ?Centro Estadual de Pesquisa em Agricultura Org?nica? ? Pesagro-Rio, Serop?dica-RJ. There are few reports of molecular diagnostic techniques for this agent. The study was conducted during the rainy and dry seasons and collected a total of 1607 blood samples, initially every three days and processed using indirect ELISA test and PCR. Percent values for A. marginale seroprevalence as function of age were tested using the ?2 test at 5% significance level. The prevalence of anti-A. marginale antibodies were 39.8% in calves aged less than 30 days, 23.3% between 30 and 60 days, 27.3% between 60 and 120 days and 38.2% between 120 and 180 days, with 31.4% for samples of all age group (180 days). Calves aged between 30 and 60, 60 and 120 and 120 and 180 days were respectively 1.90, 1.75 and 1.55 more likely to be seronegative for A. marginale than newborn ones. All calves were positive to PCR until 13 days old. The values show that during the study calves had low levels of antibodies to A. marginale, a condition that predisposes them to the development of clinical anaplasmosis. In addition, the herd was considered unstable epidemiologically to A. marginale infection. The results show that animals had low antibody titers of IgG anti-A. marginale, being more susceptible to develop clinical anaplasmosis from 30 to 60 days. The results of the PCR method confirmed A. marginale in all animals before they are 15 days old and suggest the possibility of transplacental transmission occurs in the herd.
As atividades relacionadas a bovinocultura leiteira em sistemas de alta produtividade contribuem cada dia mais com o desequil?brio sa?de-doen?a, e aumentam a probabilidade de enfermidades compat?veis com as ectoparasitoses e as infec??es transmitidas por estes vetores. Os objetivos do trabalho foram detectar a infec??o por Anaplasma marginale de forma precoce e avaliar o n?vel de anticorpos IgG anti-A. marginale em bezerros nativos e naturalmente parasitados por Rhipicephalus microplus na Baixada Fluminense, estado do Rio de Janeiro. S?o escassos os relatos sobre t?cnicas de diagn?stico molecular para este agente. Um total de 41 bezerras foi acompanhado do nascimento aos 180 dias de idade. Os animais pertenciam ao Centro Estadual de Pesquisa em Agricultura Org?nica da Pesagro-RJ. O estudo foi conduzido nas esta??es chuvosa e seca, e foram coletados um total de 1607 amostras, com intervalo inicial de tr?s dias e processados utilizando o teste ELISA indireto e a t?cnica PCR. Os valores percentuais de soropreval?cia para A. marginale em fun??o da idade foram submetidos ao teste ?2 a 5% de signific?ncia. A preval?ncia de anticorpos anti-A. marginale nos bezerros, em fun??o da idade, foi de 39,8% do soro de animais com idade inferior a 30 dias, 23,3% entre 30 e 60 dias, 27,3% entre 60 e 120 dias e 38,2% entre 120 e 180 dias, com um percentual de 31,4% para as amostras de todo o grupo et?rio (180 dias). Bezerros com idade 30 - 60, 60 - 120 e 120 - 180 dias apresentaram respectivamente 1,90, 1,75 e 1,55 mais risco de serem soronegativos para A. marginale do que os animais rec?m nascidos. Os bezerros foram diagnosticados positivos a PCR em no m?ximo 13 dias de idade. Os valores demonstram que os animais estudados apresentaram baixos t?tulos de anticorpos da classe IgG anti-A. marginale, sendo mais suscet?veis a desenvolverem anaplasmose cl?nica entre 30 a 60 dias de vida. Os resultados do m?todo PCR comprovaram a circula??o de A. marginale em todos os animais antes de completarem 15 dias de vida e sugerem a possibilidade de ocorrer transmiss?o transplacent?ria no rebanho estudado.
28
Lund, Helen Louise. "A novel platform for creating digital PCR assays to detect genetic translocations and its application to the initial diagnosis of cancer." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57026.
Full textAbstract:
Chromosomal translocations can cause cancer, often through the formation of fusion genes that code for an unnatural tyrosine kinase that promotes constitutive activation of a signaling pathway controlling cell proliferation and differentiation. For example, the diagnostic hallmark of chronic myelogenous leukemia (CML) is an oncogene fusion formed from a reciprocal translocation (t(9;22)(q34.1;q11.2)) between chromosomes 9 and 22 that results in an altered chromosome 22q known as the Philadelphia chromosome. Approximately 95% of all CML patients harbor the gene fusion, BCR-ABL, which is formed via a double stranded break (DSB) within both the Abelson oncogene 1 (ABL) on chromosome 9q, which codes for a non-receptor tyrosine kinase (ABL), and the breakpoint cluster region gene (BCR) on chromosome 22q. BCR-ABL encodes a constitutively active tyrosine kinase BCR-ABL responsible for the uncontrolled proliferation associated with chronic myelogenous leukemia. The identification of these translocation events and/or associated fusion genes in clinical samples is critical to ensure the appropriate treatment for patients where the drug and related course of therapy target an activated fusion kinase. Clinical detection of complex chromosomal rearrangements is often conducted using fluorescence in situ hybridization (FISH). The FISH analysis, though effective, offers relatively poor sensitivity while being expensive, time-consuming and technically challenging to perform.
Here we have developed and validated a new general platform for creating assays against complex chromosomal rearrangements, including both reciprocal and non-reciprocal translocations. It utilizes droplet digital PCR (ddPCR) technology in lieu of FISH to quantify the rearrangement of proto-oncogenes that undergo rearrangement as part of the translocation event. The platform is applied to the creation of two new assays of potential clinical use in cancer diagnostics or theranostics. The first provides a reliable and sensitive measure of DSBs within the major breakpoint region of BCR (M-BCR), permitting initial diagnosis of CML through unequivocal detection of the BCR-ABL fusion gene to a frequency of 0.25%. The second provides for the highly sensitive detection of DSBs in the anaplastic lymphoma kinase (ALK) gene that result in a non-reciprocal (inversion) translocation (inv(2)(p21;p23)) associated with an ALK-positive non-small cell lung cancer (NSCLC).Science, Faculty of
Graduate
29
Dubský, Milan. "Simulace biometrických zabezpečovacích systémů pracující na základě rozpoznávání tváře." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217325.
Full textAbstract:
The aim of this work is to realize a system in the Matlab-Simulink environment, which will be able to detect and recognize the human face from the input image. The created model will actually simulate the biometric security systems working on the principle of face recognition. The work is divided into two parts. In the first part, several methods for face detection from image are described. We focused on the symptomatic oriented and color segmentation methods. The pattern matching method is also described and implemented; the advantage ofthe pattern matching that it can be used either for face detection or face recognition. The second part of this work contains a description of the face recognition. Where PCA (Principal Component Analysis) are used for this task, this part of the work also includes experimental results of tests performed on our methods.
30
Mphuthi, Malekoba Batseba Nthabisheng. "Development of a real-time PCR assay to detect the fusion gene of the D26 strain of a commercial avian avulavirus 1." Diss., University of Pretoria, 2009. http://hdl.handle.net/2263/67823.
Full textAbstract:
Newcastle disease (ND), caused by avian avulavirus 1 (AA1), an enveloped, negative sense, single stranded RNA virus belonging to the Paramyxoviridae family. ND is found world-wide and leads to severe economic losses from mortality and condemnation of carcasses. Virulent ND causes clinical signs such as respiratory distress, central nervous signs, drop in egg production, weakness, gastro-intestinal symptoms and death. The disease is listed by the World Organisation for Animal Health (OIE) and outbreaks require reporting to the OIE. The OIE requires a definitive diagnosis of virulent AA1 to enable effective control of an outbreak by strict control measures and trade restrictions.
Currently the real-time reverse transcription polymerase chain reaction (RRT-PCR) assay used to diagnose ND does not differentiate between field and vaccine strain. The aim of this study was to develop and optimise a real time RT-PCR assay that detects chickens vaccinated with Vectormune® HVT NDV vaccine based on the F gene of the D26 strain. NDV F gene sequences were downloaded from Genbank® and aligned. A region unique to the D26 strain, between nucleotides 69 to 131 (using accession number M24692 for numbering) was identified and a TaqMan® MGB™ assay was developed. Primer and probe concentrations were optimised at 200 nM.
Nucleic acid was purified using a MagMax™ Pathogen RNA/DNA extraction kit and a MagMax™ Express Magnetic Particle Processor (ThermoFisher Scientific). TaqMan Fast Advanced Master Mix PCR reagents were used to amplify the AA1 F gene with one StepOnePlus Real-time PCR system.
The PCR efficiency was calculated to be 81.8% with 0.9942 coefficient correlation (R2). The 95% limit of detection was 10-1.31 plaque forming units per reaction. The assay was specific and did not detect any other AA1 isolates tested.
Twenty-four spleen impression smear field samples from chickens (12 Vectormune® HVT NDV vaccine samples and 12 vaccinated with ND virus conventional vaccine) preserved on Whatman® FTA cards, were collected between day 21 and 28 post vaccination. The assay detected only the D26 vaccine strain and was negative when tested on other field samples.
The developed real time PCR was sensitive, reliable and repeatable and will also be able to produce results rapidly as compared to other conventional methods.Dissertation (MSc)--University of Pretoria, 2018.
Veterinary Tropical Diseases
MSc
Unrestricted
31
Soares, Rosilene Calazans. "Estudo da detec??o do DNA do papiloma v?rus humano (HPV) e da express?o imuno-histoqu?mica de prote?na do ciclo celular no carcinoma epiderm?ide oral." Universidade Federal do Rio Grande do Norte, 2005. http://repositorio.ufrn.br:8080/jspui/handle/123456789/17157.
Full textAbstract:
Made available in DSpace on 2014-12-17T15:32:31Z (GMT). No. of bitstreams: 1
RosileneCS.pdf: 376311 bytes, checksum: 8a0459347381c3b197002ddd72f02225 (MD5)
Previous issue date: 2005-11-04Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior
Oral squamous cell carcinoma (OSCC) is the most common malignancy in oral cavity and human papillomavirus (HPV) may have an important role in its development. The aim of this experiment was to investigate the HPV DNA and viral types in 90 cases of OSCC. Moreover, a comparative analysis between the cases of OSSC with and without HPV DNA was performed by using cell cycle markers p21 and pRb in order to detect a possible correlation of these proteins and HPV infection. DNA was extracted from paraffin embedded tissue and amplified by PCR (polymerase chain reaction) with primers PCO3+ e PCO4+ for a fragment of human β-globin gene. After this procedure, PCR for HPV DNA detection was realized using a pair of generic primers GP5+ e GP6+. Immunohistochemical study was performed by streptoavidin-biotin technique and antibodies against p21 and pRb proteins were employed. Eighty-eight cases were positive for human β-globin gene and HPV DNA was found in 26 (29.5%) of then. It could not be detected significant correlation between HPV and age, sex and anatomical sites of the lesion. The most prevalent viral type was HPV 18 (80.8%). Regarding the immunohistochemical analysis, it was detected significant association between HPV presence and pRb immunoexpression (p=0,044), nevertheless, the same was not observed in relation to p21 protein (p =0,416). It can be concluded that the low detection of HPV DNA in OSCC by the present experiment suggests a possible role of the virus in the development and progression in just a subset of this disease
O carcinoma epiderm?ide oral ? a neoplasia maligna mais freq?ente da cavidade oral e o papilomav?rus humano (HPV) parece ter um relevante papel na indu??o desta les?o. Neste trabalho investigou-se o DNA do HPV e tipos virais em 90 casos de carcinoma epiderm?ide oral (CEO). Realizou-se tamb?m uma an?lise comparativa entre os grupos de CEO com DNA do HPV e sem o DNA do v?rus, empregando-se os marcadores do ciclo celular p21 e pRb, a fim de estabelecer poss?vel correla??o entre a express?o imuno-histoqu?mica dessas prote?nas e a infec??o pelo HPV. O DNA foi extra?do de tecido emblocado em parafina e amplificado por PCR (rea??o em cadeia da polimerase) com um par de primers designados PCO3+ e PCO4+ para um fragmento do gene da β-globina humana. Posteriormente, realizou-se PCR para detec??o do DNA de HPV utilizando-se um par de primers gen?ricos designados GP5+ e GP6+. A tipagem viral foi realizada pela hibridiza??o dot blot. No m?todo imuno-histoqu?mico utilizou-se a t?cnica da streptavidina-biotina com um painel de anticorpos monoclonais para as prote?nas p21 e pRb. Dos 88 casos positivos para o gene da β-globina humana, em 26 (29,5%) foi detectado o DNA do HPV. N?o houve associa??o significativa entre o HPV e as vari?veis idade e sexo dos pacientes e localiza??o anat?mica da les?o. O tipo viral prevalente foi o HPV 18 (80,8%). Quanto ? an?lise imuno-histoqu?mica, foi observada associa??o estatisticamente significativa entre a presen?a do HPV e a express?o imunohistoqu?mica de pRb (p=0,044), entretanto, n?o houve qualquer diferen?a estatisticamente significativa entre a express?o da prote?na p21 e a presen?a do v?rus (p =0,416). P?de-se concluir que o baixo percentual de detec??o do DNA do HPV no carcinoma epiderm?ide oral no presente trabalho, sugere uma poss?vel participa??o do HPV no desenvolvimento e progress?o de apenas um subgrupo dessas les?es
32
Lima, Fabio Soares de. "Detec??o e classifica??o de modos de opera??o do bombeio mec?nico via cartas dinamom?tricas." Universidade Federal do Rio Grande do Norte, 2014. http://repositorio.ufrn.br:8080/jspui/handle/123456789/15257.
Full textAbstract:
Made available in DSpace on 2014-12-17T14:55:21Z (GMT). No. of bitstreams: 1
FabioSL_TESE.pdf: 5888891 bytes, checksum: cd954df5e4af671c3361060293cc5710 (MD5)
Previous issue date: 2014-05-30Universidade Federal do Rio Grande do Norte
The precision and the fast identification of abnormalities of bottom hole are essential to prevent damage and increase production in the oil industry. This work presents a study about a new automatic approach to the detection and the classification of operation mode in the Sucker-rod Pumping through dynamometric cards of bottom hole. The main idea is the recognition of the well production status through the image processing of the bottom s hole dynamometric card (Boundary Descriptors) and statistics and similarity mathematics tools, like Fourier Descriptor, Principal Components Analysis (PCA) and Euclidean Distance. In order to validate the proposal, the Sucker-Rod Pumping system real data are used
A identifica??o r?pida e precisa de anormalidades de fundo de po?o ? essencial para evitar danos e aumentar a produ??o na ind?stria do petr?leo. Esta tese apresenta um estudo sobre uma nova abordagem autom?tica para a detec??o e classifica??o de modos de opera??o no sistema de Bombeio Mec?nico atrav?s de carta de dinamom?tricas de fundo de po?o. A id?ia principal ? o reconhecimento das condi??es de produ??o do sistema atrav?s do processamento de imagem do carta dinamom?trica de fundo de po?o (Descritores de Fourier) e ferramentas matem?ticas estat?sticas (An?lise de Componentes Principais - PCA) e de similaridade (Dist?ncia Euclidiana). Para validar a proposta, s?o utilizados dados provenientes de sistemas de Bombeio Mec?nico reais
33
Jablaoui, Cherif. "La texturation par detente instantannée [sic] controlée DIC dans le developpements [sic] de nouvelles opérations d’extraction d’huiles des graines oleagineuses." Thesis, La Rochelle, 2018. http://www.theses.fr/2018LAROS015/document.
Full textAbstract:
Durant les dernières années, les études des procédés de trituration ou d’extraction des huiles végétales à partir des graines oléagineuses se sont concentrées sur les impacts environnementaux, la consommation énergétique et les qualités nutritionnelles. Les travaux de recherche ont donc visé l’étude de l’intensification des procédés d’extraction des huiles et de leur raffinage à travers leur décontamination phytosanitaire, grâce à la texturation par Détente Instantanée Contrôlée DIC et à la technologie d’autovaporisation multi-flash (MFA), respectivement. D’une part, ces travaux de recherche ont porté sur l’impact d’une texturation par la technologie de DIC, qui est à l’origine d’une modification de l’aptitude technologique des graines, vis-à-vis de l’extraction et de la préservation de la qualité nutritionnelle des huiles extraites. Appliquée sur des graines oléagineuses (graines de colza et de soja) et sur la base d’un bilan quantitatif (rendements en huile), cette étude met en évidence l’impact de la technologie DIC comparée à des prétraitements conventionnels tels que la cuisson, le concassage, l’aplatissage et le traitement par expandeur. D’autre part, sur la base d’une modélisation phénoménologique, les cinétiques d'extraction ont aussi été étudiées en vue de comparer les paramètres cinétiques d'extraction d'huiles végétales, cités plus haut, tels que la diffusivité effective et l’accessibilité initiale, par rapport aux systèmes conventionnels. Les résultats indiquent l’amélioration de la cinétique d'extraction des matières traitées par DIC d'une part, et la préservation de la qualité nutritionnelle des composés extraits, d'autre part. Enfin, les travaux fondés sur la technologie d’autovaporisation multi-flash (MFA) ont porté sur le raffinage des huiles extraites principalement au plan de son contenu phytosanitaire. L’efficacité de cette technologie a été prouvée dans l’objectif d’une décontamination des résidus de pesticides (organochlorés) dans le cas de l’huile de colza bruteRecently, studies of structural pretreatment and improvement of the sector of vegetal oil extraction from oilseeds have focused on the intensification of the concerned processes in both aspects of performance (environmental impacts, energy consumption, and kinetics) and quality (nutritional content, sensorial attributes…). Therefore, this research aimed to study the improvement of oil extraction processes and their phytosanitary decontamination, based on DIC texturing and multi-flash autovaporization (MFA), respectively. First, the effect DIC texturing technology was studied as structural pretreatments in improving the technological aptitudes of processing seeds such as rapeseed and soybean, regarding the extraction kinetics and yields, and the preservation of the nutritional composition of the extracted oils. DIC was studied based on quantitative assessments of oil (yields, composition…), highlighting its impact following the conventional pretreatments such as cooking, crushing, flattening, and expanding. Furthermore, founded on the Coupled Washing Diffusion CWD phenomenological model, in the specific cases of Negligible External Resistance NER extraction kinetics were also conducted to determine the fundamental extraction parameters of solvent vegetal oil extraction kinetics, such as the effective diffusivity and the starting accessibility, compared to conventional systems. The results denoted the improvement of extraction kinetics of the materials treated with DIC, on the one hand, and the preservation of the nutritional quality of the extracted compounds, on the other hand. Finally, this work focused on the study of multi-flash autovaporization (MFA) technology and its effect on the phytosanitary aspect of the extracted oils. The effectiveness of this technology has been proven for pesticide residue decontamination (organochlorine) of crude rapeseed oil
34
Cattani, Fernanda. "Detec??o e quantifica??o de c?lulas vi?veis de Bacillus sporothermodurans e de Bacillus cereus em leite atrav?s de PCR convencional e de PCR em tempo real associadas ao prop?dio monoazida." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2012. http://tede2.pucrs.br/tede2/handle/tede/5453.
Full textAbstract:
Made available in DSpace on 2015-04-14T14:51:22Z (GMT). No. of bitstreams: 1
445049.pdf: 985835 bytes, checksum: 6999f9525f1cf085ddde3a6a0fb67e0b (MD5)
Previous issue date: 2012-10-31The presence of Bacillus spp. in milk is an important problem for the dairy industry due to their capability of sporulation and the possibility of spore resistance to heat treatment by ultra high temperature (UHT). Bacillus sporothermodurans survive to the UHT system, germinating and growing in stored milk and, if not correctly identified and quantified, can exceed the criterion established for mesophilic aerobic, besides altering the quality of dairy products when in high concentrations. On the other hand, contamination of milk by Bacillus cereus is not only an important cause of deterioration, but is also associated with the occurrence of diarrhea and emetic syndromes. Traditionally, these microorganisms are identified and quantified in food using conventional microbiological techniques, but the Polymerase Chain Reaction (PCR) based methods have been widely used for the same purpose. However, PCR cannot distinguish between viable and dead cells, which can be overcame with the use of DNA intercalating, such as propidium monoazide (PMA). PMA binds to DNA derived from cells with damaged membranes, preventing their amplification by PCR, allowing, thus, the selective detection of viable cells. Therefore, this thesis aimed to characterize the thermal resistance of B. sporothermodurans and to develop methods of detection and quantificatification of viable cells of B. sporothermodurans and B. cereus in milk samples by qPCR associated with PMA. Isothermal and non-isothermal treatments allowed the determination of the profile of heat resistance of B. sporothermodurans spores to heat UHT process, predicting that to 121?C was found a D value between 2 a 4 min. The selective detection and quantification of B. sporothermodurans and B. cereus by PMA-qPCR were developed targeting 16S rRNA gene and hemolysin gene, respectively.The treatment with PMA from pure culture and artificially contaminated UHT milk were standardized by end-point PCR for the detection of viable cells of these microorganisms. The inhibition of amplification of DNA from dead cells was obtained at a concentration of 30μg/mL PMA. The standardization of qPCR assays were performed using hydrolysis probes (TaqMan? system) specific to each target gene. The quantification limit from UHT milk artificially contaminated was 2.5 x 102 CFU/mL for B. sporothermodurans and 7.5 x 102 CFU/mL for B. cereus. The assays were applied to 135 samples of UHT milk of different commercial brands, comparing with the conventional method of cultivation for each microorganism. B. sporothermodurans and B. cereus were respectively detected in 14 (10.4%) and 44 (32.6%) of the samples by molecular methods developed, and in 11 (8.1%) and 15 (11.1%) by conventional culturing methods. The PMA-qPCR methods developed in this study were specific and sensitive for the detection and quantification of viable B. sporothermodurans and B. cereus cells, being applicable for the evaluation of milk samples, reducing the time for the analysis of this product. Furthermore, the results showed that B. cereus can be found in UHT milk
A presen?a de Bacillus spp. em leite representa um importante problema para a ind?stria de latic?nios devido ? sua capacidade de esporula??o e ? possibilidade de resist?ncia do esporo ao tratamento t?rmico por ultra alta temperatura (UAT). O Bacillus sporothermodurans sobrevive ao sistema UHT, germinando e se multiplicando no leite estocado e, caso n?o seja corretamente quantificado e identificado, pode ultrapassar o limite estabelecido pela legisla??o para microrganismos mes?filos aer?bios, al?m de alterar a qualidade dos produtos l?cteos quando em altas concentra??es. Por outro lado, a contamina??o de leite por Bacillus cereus constitui n?o somente uma importante causa de deteriora??o, mas tamb?m est? associada com a ocorr?ncia das s?ndromes em?tica e diarreica. Tradicionalmente, estes microrganismos s?o identificados e quantificados em alimentos atrav?s de t?cnicas cl?ssicas de cultivo, mas m?todos baseados na Rea??o em Cadeia pela Polimerase (PCR) tamb?m t?m sido amplamente utilizados. Entretanto, a PCR n?o distingue c?lulas mortas de c?lulas vi?veis, o que pode ser contornado com o emprego de intercalantes de DNA, como o prop?dio monoazida (PMA). O PMA se liga ao DNA derivado de c?lulas com membranas rompidas, impedindo suas amplifica??es na PCR, permitindo, assim, a detec??o seletiva de c?lulas vi?veis. Portanto, a presente tese teve por objetivo caracterizar a resist?ncia t?rmica de B. sporothermodurans, bem como desenvolver m?todos de detec??o e quantifica??o de c?lulas vi?veis de B. sporothermodurans e de B. cereus em amostras de leite atrav?s de PCR associada ao PMA. Tratamentos isot?rmicos e n?o isot?rmicos permitiram a determina??o do perfil de resist?ncia t?rmica de esporos de B. sporothermodurans ao processo UHT, predizendo que a 121?C foi encontrado um valor D entre 2 a 4 min.A detec??o e quantifica??o seletivas de B. sporothermodurans e de B. cereus atrav?s de PMA-qPCR foram desenvolvidas utilizando o gene RNAr 16S e o gene da hemolisina como alvos, respectivamente. O tratamento com PMA a partir de cultura pura e leite UHT artificialmente contaminado foi padronizado atrav?s da PCR convencional para a detec??o de c?lulas vi?veis destes microrganismos. A inibi??o da amplifica??o de DNA de c?lulas mortas foi obtida na concentra??o de 30μg/mL de PMA. A padroniza??o dos ensaios de qPCR foram realizados utilizando sondas de hidr?lise (sistema TaqMan?) espec?ficas para cada gene alvo. O limite de quantifica??o a partir de leite UHT artificialmente contaminado foi de 2,2 x 102 UFC/mL para B. sporothermodurans e de 7,5 x 102 UFC/mL para B. cereus. As t?cnicas foram aplicadas a 135 amostras de leite UHT de diferentes marcas comerciais, comparando com a metodologia cl?ssica de cultivo para cada microrganismo. B. sporothermodurans e B. cereus foram, respectivamente, detectados em 14 (10,4%) e 44 (32,6%) das amostras analisadas pelos m?todos moleculares desenvolvidos, e em 11 (8,1%) e 15 (11,1%) pelos m?todos convencionais de cultivo. Os m?todos de PMA-qPCR desenvolvidos neste estudo foram espec?ficos e sens?veis para a detec??o e quantifica??o de c?lulas vi?veis de B. sporothermodurans e de B. cereus, mostrando-se aplic?veis para serem utilizados na avalia??o de amostras de leite, reduzindo o tempo de an?lise deste produto. Al?m disso, os resultados demonstraram que B. cereus pode ser encontrado em leite tratado pelo sistema de UHT
35
Musil, Martin. "Přenosy rastrových dat v FPGA." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2012. http://www.nusl.cz/ntk/nusl-236507.
Full textAbstract:
This work deals with the design and implementation of high-speed communication interfaces into FPGA chip and their utilizing for image transmission and processing. In the implementation part has been created PCI Express endpoint device, which provides data transfers between the FPGA chip and computer RAM memory. As a source of image data for further processing was connected the Unicam M621 camera throught the Ethernet interface to FPGA chip. The project was implemented on the Xilinx SP605 development board. Using both of the the interfaces were demonstrated on the application of edge detection using Sobel operator. The PCI Express endpoint device driver for the Linux operating system and a simple application interface in C language was also created within this project.
36
Radhakrishnan, Anirudh. "Automated pavement condition analysis based on AASHTO guidelines." Thesis, Manhattan, Kan. : Kansas State University, 2009. http://hdl.handle.net/2097/1920.
Full text
37
Jošth, Radovan. "Využití GPU pro algoritmy grafiky a zpracování obrazu." Doctoral thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-261274.
Full textAbstract:
Táto práca popisuje niekoľko vybraných algoritmov, ktoré boli primárne vyvinuté pre CPU procesory, avšak vzhľadom k vysokému dopytu po ich vylepšeniach sme sa rozhodli ich využiť v prospech GPGPU (procesorov grafického adaptéra). Modifikácia týchto algoritmov bola zároveň cieľom nášho výskumu, ktorý bol prevedený pomocou CUDA rozhrania. Práca je členená podľa troch skupín algoritmov, ktorým sme sa venovali: detekcia objektov v reálnom čase, spektrálna analýza obrazu a detekcia čiar v reálnom čase. Pre výskum detekcie objektov v reálnom čase sme zvolili použitie LRD a LRP funkcií. Výskum spektrálnej analýzy obrazu bol prevedný pomocou PCA a NTF algoritmov. Pre potreby skúmania detekcie čiar v reálnom čase sme používali dva rôzne spôsoby modifikovanej akumulačnej schémy Houghovej transformácie. Pred samotnou časťou práce venujúcej sa konkrétnym algoritmom a predmetu skúmania, je v úvodných kapitolách, hneď po kapitole ozrejmujúcej dôvody skúmania vybranej problematiky, stručný prehľad architektúry GPU a GPGPU. Záverečné kapitoly sú zamerané na konkretizovanie vlastného prínosu autora, jeho zameranie, dosiahnuté výsledky a zvolený prístup k ich dosiahnutiu. Súčasťou výsledkov je niekoľko vyvinutých produktov.
38
Germano, Amanda Lucena. "An?lise de desempenho de abordagens orientadas a fluxo de dados aplicadas ? detec??o de falhas de processos industriais." PROGRAMA DE P?S-GRADUA??O EM ENGENHARIA EL?TRICA E DE COMPUTA??O, 2017. https://repositorio.ufrn.br/jspui/handle/123456789/24547.
Full textAbstract:
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-01-15T21:33:32Z
No. of bitstreams: 1
AmandaLucenaGermano_DISSERT.pdf: 7217536 bytes, checksum: 25a20d10202bb0af3f3b8e89539d2fbb (MD5)Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-01-17T12:52:40Z (GMT) No. of bitstreams: 1 AmandaLucenaGermano_DISSERT.pdf: 7217536 bytes, checksum: 25a20d10202bb0af3f3b8e89539d2fbb (MD5)
Made available in DSpace on 2018-01-17T12:52:40Z (GMT). No. of bitstreams: 1 AmandaLucenaGermano_DISSERT.pdf: 7217536 bytes, checksum: 25a20d10202bb0af3f3b8e89539d2fbb (MD5) Previous issue date: 2017-07-31
Com a necessidade do aumento da qualidade dos produtos e do desempenho dos processos, o grau de automa??o cresceu bastante nas ind?strias. Com isso, os sistemas est?o cada vez mais complexos e v?m acompanhados por problemas dif?ceis de resolver devido ? alta dimensionalidade desses sistemas e do grande volume do fluxo de informa??es necess?rias, al?m da aleatoriedade de falhas e defeitos. Uma falha inesperada pode levar a riscos operacionais, por isso a import?ncia de detectar e localizar a falha, principalmente quando a planta industrial ainda est? operando em uma regi?o control?vel e ? poss?vel agir para trazer o processo de volta para o estado normal, seguro e operacional. Assim, ? desej?vel que o sistema de detec??o de falhas forne?a respostas r?pidas e confi?veis com um esfor?o computacional adequado para processamento em tempo real, mesmo necessitando tratar com grandes quantidades de dados. Para trabalhar com grandes quantidades de dados em tempo real, surgiu o modelo de fluxo de dados, que consiste de uma sequ?ncia ordenada de pontos que s? podem ser lidos apenas uma ou algumas poucas vezes. Essa ?rea cresceu bastante nos ?ltimos anos, principalmente devido a grande quantidade de sistemas que precisavam tratar com dados desse tipo, que incluem desde dados do mercado financeiro, registros telef?nicos, transa??es web a dados m?dicos, redes de sensores ou mesmo dados multim?dia. Diante da relev?ncia do tema de detec??o de falhas, nessa tese foram utilizados o TEDA (Typicality and Eccentricity Data Analytics), o RDE (Recursive Density Estimation) e o R-PCA (Recursive Principal Component Analysis) como ferramentas para detec??o de falhas em processos industriais. Para a an?lise do desempenho de cada uma dessas abordagens foi utilizado o cl?ssico benchmark Tennessee Eastman Process.
In order to increase product quality and process performance, the degree of automation has grown significantly in industries. As a result, systems are increasingly complex and are accompanied by problems that are difficult to solve due to the high dimensionality of these systems and the large amount of information flow, as well as the randomness of faults and defects. An unexpected failure can lead to operational risks, so the importance of detecting and locating the fault, especially when the industrial plant is still operating in a controllable region and it is possible to act to bring the process back to normal, safe and operational. Thus, it is desirable for the fault detection system to provide fast and reliable responses with a computational effort appropriate for real-time processing, even though it requires handling large amounts of data. In this context, data stream-oriented algorithms to outlier detection may be promising candidates for fault detection of industrial process, because they work with sequences of temporarily ordered samples. In addition, they handle well with large amount of data because they are recursive and online algorithms that do not need to store past samples. Thus, in this dissertation two algorithms of this class are analyzed, named TEDA (Typicality and Eccentricity Data Analytics) and RDE (Recursive Density Estimation), when applied to fault detection of industrial processes. Their performances are compared to R-PCA (Recursive Principal Component Analysis) algorithm. The classic Tennessee Eastman Process benchmark was used as case study to evaluate these algorithms.
39
Hauser, Václav. "Rozpoznávání obličejů v obraze." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-219434.
Full textAbstract:
This master thesis deals with the detection and recognition of faces in the image. The content of this thesis is a description of methods that are used for the face detection and recognition. Method described in detail is the principal component analysis (PCA). This method is subsequently used in the implementation of face recognition in video sequence. In conjunction with the implementation work describes the OpenCV library package, which was used for implementation, specifically the C ++ API. Finally described application tests were done on two different video sequences.
40
Silva, Claudia Bezerra da. "Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??ode qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba." Universidade Federal Rural do Rio de Janeiro, 2016. https://tede.ufrrj.br/jspui/handle/jspui/2107.
Full textAbstract:
Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-19T13:49:16Z
No. of bitstreams: 1
2016 - Claudia Bezerra da Silva.pdf: 8032175 bytes, checksum: ef71dd2a0e7801e9000e116c822a3a00 (MD5)Made available in DSpace on 2017-10-19T13:49:16Z (GMT). No. of bitstreams: 1 2016 - Claudia Bezerra da Silva.pdf: 8032175 bytes, checksum: ef71dd2a0e7801e9000e116c822a3a00 (MD5) Previous issue date: 2016-03-11
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
and investigate the circulation of this agent in dogs in the Itaguai microregion, Rio de Janeiro, Brazil, and dogs and ticks in two provinces of the island of Cuba, analyzing epidemiological aspects associated with infections caused by this bacterium in dogs. A new real-time polymerase chain reaction method (qPCR) was patterned to target the citrate synthase gene (gltA) for the identification of A. platys in naturally infected dogs. The primers and probe were designed to amplify a fragment of 84 base pairs based on gltA gene sequences of A. platys available in GenBank. 186 blood samples of dogs from Itaguai microregion, Rio de Janeiro, Brazil, were tested by qPCR. The same samples were tested by cytology and nested polymerase chain reaction (nPCR, 16S rDNA) to determine the performance of qPCR front of these techniques. 17.20% of the samples tested positive by qPCR were significantly more than that detected by nPCR (13.98%). The qPCR technique was more specific than cytology, due to false-positive results obtained by optical microscopy. The prevalence of A. platys in dogs from Itaguai microregion was 14.4%. Dogs less than six months, infested by ticks, that spend the most of the time restrict to domestic environment and without shelter are factors associated with infection by this hemoparasite in dogs in the study area. During research, A. platys held in Cuba, 100 blood samples were collected from residents dogs in four cities located in the provinces of Havana and Mayabeque. When inspecting the animals, found ticks were collected, identified and carefully grouped, forming a total of 49 pools. DNA extracted from blood samples from dogs and ticks were subjected nPCR (16S rDNA). Positive samples in nPCR were also subjected to conventional PCR (gltA gene), and the products were sequenced. Only the species Rhipicephalus sanguineus sensu lato was found in Cuban dogs and 10.2% (n=5/49) of these ticks added to 16.0% (n=16/100) dogs were considered positive for A. platys. All sequences analyzed of the gltA and 16S rDNA genes, respectively, showed a 99-100% identity with sequences from A. platys reported in other countries. Phylogenetic analysis showed two clusters defined for the 16S rDNA gene and three clusters defined for the gltA gene. Based on the gltA gene, the deduced amino acid sequence showed two points of non-synonymous mutations at positions 88 and 168 compared to the reference sequence DQ525687. A preliminary study on the epidemiological aspects associated with infection with A. platys showed no statistical association with the variables studied (p> 0.05). This study also to report the first evidence of A. platys in both dogs and ticks in Cuba also presents for the first time the development of a new qPCR method that contributes to the advancement of research involving A. platys. The epidemiological study in Brazil allowed us to identify significant factors in the occurrence of canine anaplasmosis, while in Cuba, it can be concluded that more research is needed to assess what the deciding factors in the transmission and spread of A. platys in that country.
platys, e investigar a circula??o deste agente em c?es na microrregi?o de Itagua?, Rio de Janeiro, Brasil, e c?es e carrapatos em duas prov?ncias da ilha de Cuba, analisando aspectos epidemiol?gicos associados ? infec??o causada por esta bact?ria em c?es. Um novo m?todo de rea??o em cadeia da polimerase em tempo real (qPCR) foi padronizado com alvo no gene citrato sintase (gltA) para a identifica??o de A. platys em c?es naturalmente infectados. Os oligoiniciadores e a sonda foram desenhados para amplificar um fragmento de 84 pares de base baseado em sequ?ncias do gene gltA de A. platys dispon?veis no GenBank. 186 amostras de sangue de c?es da microrregi?o de Itagua?, Rio de Janeiro, Brasil, foram testados pela qPCR. As mesmas amostras foram testadas pela citologia e rea??o em cadeia da polimerase nested (nPCR, 16S rDNA) para determinar o desempenho da qPCR frente ? essas t?cnicas. 17,20% das amostras testadas pela qPCR foram positivas, significativamente mais do que detectado pela nPCR (13,98%). A t?cnica de qPCR foi mais espec?fica que a citologia, em virtude dos resultados falsopositivos obtidos pela microscopia ?ptica. A preval?ncia de A. platys em c?es da microrregi?o de Itagua? foi de 14,4%. C?es com menos de seis meses, infestados por carrapatos, que possam maior tempo restrito ao ambiente dom?stico e sem abrigo s?o fatores associados a infec??o por este hemoparasito em c?es na regi?o do estudo. Durante investiga??o de A. platys realizada em Cuba, 100 amostras de sangue foram coletadas de c?es residentes em quatro cidades localizadas nas prov?ncias de Habana e Mayabeque. Ao inspecionar os animais, carrapatos encontrados foram coletados, identificados e criteriosamente agrupados, formando um total de 49 pools. Amostras de DNA extra?das do sangue dos c?es e de carrapatos foram submetidas a nPCR (16S rDNA). Amostras positivas na nPCR foram tamb?m submetidas a PCR convencional (gene gltA), e os produtos foram sequenciados. Somente a esp?cie Rhipicephalus sanguineus sensu lato foi encontrada em c?es cubanos, e 10,2% (n=5/49) desses carrapatos somado aos 16,0% (n=16/100) de c?es foram considerados positivos para A. platys. Todas as sequ?ncias analisadas dos genes gltA e 16S rDNA, respectivamente, mostraram uma identidade de 99-100% com sequ?ncias de A. platys reportadas em outros pa?ses. A an?lise filogen?tica mostrou dois clusters definidos para o gene 16S rDNA e tr?s clusters definidos para o gene gltA. Com base no gene gltA, a sequ?ncia de amino?cidos deduzidos demonstrou dois pontos de muta??es n?o-sin?nimas nas posi??es 88 e 168 comparados com sequ?ncia de refer?ncia DQ525687. Um estudo preliminar sobre os aspectos epidemiol?gicos associados com a infec??o por A. platys demonstrou nenhuma associa??o estat?stica com as vari?veis avaliadas (p > 0,05). O presente estudo al?m de relatar a primeira evid?ncia de A. platys em ambos c?es e carrapatos em Cuba, tamb?m apresenta pela primeira vez o desenvolvimento de um novo m?todo de qPCR que contribui para o avan?o da pesquisa envolvendo A. platys. O estudo epidemiol?gico realizado no Brasil permitiu identificar fatores importantes na ocorr?ncia da anaplasmose canina, enquanto em Cuba, pode-se concluir que mais investiga??es s?o necess?rias para avaliar quais os fatores decisivos na transmiss?o e dispers?o de A. platys nesse pa?s.
41
Seguy, françoise. "Evaluation du metabolisme anaerobie dans cinq sports differents (aviron, basket-ball, cyclisme, ski alpin et tennis) par la determination de la relation force-vitesse et la detente verticale." Lyon 1, 1991. http://www.theses.fr/1991LYO1M340.
Full text
42
Medeiros, Thatiany Ara?jo de. "Avalia??o dos m?todos sorol?gicos e da t?cnica de Nested-PCR utilizando o iniciador GRA7 na detec??o do Toxoplasma gondii no l?quido amni?tico de gestantes." PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS BIOL?GICAS, 2016. https://repositorio.ufrn.br/jspui/handle/123456789/22007.
Full textAbstract:
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-13T20:11:14Z
No. of bitstreams: 1
ThatianyAraujoDeMedeiros_DISSERT.pdf: 1620239 bytes, checksum: e75f59b47b08a1483c79c95ce336be37 (MD5)Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-02-16T00:30:20Z (GMT) No. of bitstreams: 1 ThatianyAraujoDeMedeiros_DISSERT.pdf: 1620239 bytes, checksum: e75f59b47b08a1483c79c95ce336be37 (MD5)
Made available in DSpace on 2017-02-16T00:30:21Z (GMT). No. of bitstreams: 1 ThatianyAraujoDeMedeiros_DISSERT.pdf: 1620239 bytes, checksum: e75f59b47b08a1483c79c95ce336be37 (MD5) Previous issue date: 2016-04-08
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)
A toxoplasmose tem como agente etiol?gico o protozo?rio Toxoplasma gondii e geralmente causa infec??o assintom?tica em indiv?duos imunocompetentes. Entretanto, na transmiss?o cong?nita pode gerar quadros graves e morte, representando um s?rio problema de sa?de p?blica. Em pacientes gr?vidas reativas para IgM anti-T. gondii recomenda-se diagn?stico molecular do l?quido amni?tico utilizando a metodologia de PCR qualitativa com o iniciador B1. Por?m, seu diagn?stico utilizando esta metodologia pode ser ineficiente principalmente na detec??o de cepas h?bridas encontradas na Am?rica Latina. Nesse contexto, o objetivo deste trabalho foi avaliar a efici?ncia dos m?todos sorol?gicos e da t?cnica de Nested-PCR utilizando o iniciador GRA7 na detec??o do Toxoplasma gondii na transmiss?o cong?nita. O estudo foi realizado em 71 gestantes, atendidas no Centro de Sa?de Anita Garibaldi (CSAG), Maca?ba-RN no per?odo de 2011 a 2015, com idades gestacionais vari?veis e faixa et?ria entre 15-49 anos, submetidas a procedimentos de rotina durante o exame pr?-natal tratando-se, portanto, de um estudo de delineamento observacional transversal de acur?cia. Foram coletadas amostras de sangue perif?rico e l?quido amni?tico. Inicialmente foi realizado o diagn?stico sorol?gico para toxoplasmose com o aux?lio das t?cnicas de ELISA e Imunofluoresc?ncia indireta (IFI) para a detec??o de IgG e IgM anti-T. gondii. Posteriormente, as gestantes que apresentaram testes sorol?gicos reativos para IgM foram selecionadas para a realiza??o da t?cnica de Nested-PCR utilizando os iniciadores RE e GRA7. A sororeatividade detectada pela presen?a de IgG e IgM anti-.T gondii pelas t?cnicas de ELISA e IFI foram, respectivamente, 91,55% e 14,08%, 76,06% e 5,63%. A positividade total para IgM quando associado as t?cnicas de ELISA e IFI foi de 15,49% (11/71). Entretanto, a an?lise da positividade com a utiliza??o da Nested-PCR foi de 0% (0/11), 9,09% (1/11) e 54,5% (6/11) utilizando os iniciadores RE, B1 e GRA7 respectivamente. Os resultados indicam que o iniciador GRA7 proposto ? mais eficiente na detec??o do T. gondii no l?quido amni?tico que o iniciador B1 e RE, quando utilizada a t?cnica de Nested-PCR.
Toxoplasmosis is the etiologic agent protozoan Toxoplasma gondii and usually cause asymptomatic infection in immunocompetent individuals. However, congenital transmission can lead to serious and death, representing a serious public health problem. In pregnant patients reactive to IgM anti-T. gondii is recommended molecular diagnosis using amniotic fluid qualitative PCR method with the primer B1. However, the diagnosis using this approach can be inefficient mainly for detection of hybrid strains found in Latin America. In this context, the objective of this study was to evaluate the effectiveness of serological methods and nested PCR technique using the primer GRA7 the detection of Toxoplasma gondii congenital transmission. The study was performed in 71 pregnant women seen at the Anita Garibaldi Health Center (CSAG), Maca?ba-RN in the period 2011 to 2015, aged gestational variables and age group 15-49 years, subjected to routine procedures during the examination prenatal the case, therefore, a study of cross-sectional observational design accuracy. Samples of peripheral blood and amniotic fluid was collected. Initially it performed the serological diagnosis of toxoplasmosis with the help of ELISA and indirect immunofluorescence (IIF) for the detection of IgG and IgM anti-T. gondii. Subsequently, patients showed reactive serologic tests for IgM were selected to perform the nested PCR technique using the RE and GRA7 initiators. The seroreactivity detected by the presence of anti-IgG and IgM. T. gondii by the ELISA and IFA techniques were respectively 91.55% and 14.08%, 76.06% and 5.63%. Total positivity for IgM when combined the techniques of ELISA and IFA was 15.49% (11/71). However, analysis of positive using the nested PCR was 0% (0/11), 9,09% (1/11) and 54.5% (6/11) using the primers RE, B1 and GRA7 respectively. The results indicate that the proposed GRA7 initiator is more efficient in the detection of T. gondii in amniotic fluid B1 and RE primer, when using the nested PCR technique.
43
Dantas, Amanda Danielle Oliveira da Silva. "Identifica??o de modelos polinomiais narx utilizando algoritmos combinados de detec??o de estrutura e estima??o de par?metros com aplica??es pr?ticas." Universidade Federal do Rio Grande do Norte, 2013. http://repositorio.ufrn.br:8080/jspui/handle/123456789/15489.
Full textAbstract:
Made available in DSpace on 2014-12-17T14:56:16Z (GMT). No. of bitstreams: 1
AmandaDOSD_DISSERT.pdf: 3108692 bytes, checksum: 6c24c65f349d3aca5d9122aa78c3b00c (MD5)
Previous issue date: 2013-07-26A modelagem de processos industriais tem auxiliado na produ??o e minimiza??o de custos, permitindo a previs?o dos comportamentos futuros do sistema, supervis?o de processos e projeto de controladores. Ao observar os benef?cios proporcionados pela modelagem, objetiva-se primeiramente, nesta disserta??o, apresentar uma metodologia de identifica??o de modelos n?o-lineares com estrutura NARX, a partir da implementa??o de algoritmos combinados de detec??o de estrutura e estima??o de par?metros. Inicialmente, ser? ressaltada a import?ncia da identifica??o de sistemas na otimiza??o de processos industriais, especificamente a escolha do modelo para representar adequadamente as din?micas do sistema. Em seguida, ser? apresentada uma breve revis?o das etapas que comp?em a identifica??o de sistemas. Na sequ?ncia, ser?o apresentados os m?todos fundamentais para detec??o de estrutura (Modificado Gram- Schmidt) e estima??o de par?metros (M?todo dos M?nimos Quadrados e M?todo dos M?nimos Quadrados Estendido) de modelos. No trabalho ser? tamb?m realizada, atrav?s dos algoritmos implementados, a identifica??o de dois processos industriais distintos representados por uma planta de n?vel did?tica, que possibilita o controle de n?vel e vaz?o, e uma planta de processamento prim?rio de petr?leo simulada, que tem como objetivo representar um tratamento prim?rio do petr?leo que ocorre em plataformas petrol?feras. A disserta??o ? finalizada com uma avalia??o dos desempenhos dos modelos obtidos, quando comparados com o sistema. A partir desta avalia??o, ser? poss?vel observar se os modelos identificados s?o capazes de representar as caracter?sticas est?ticas e din?micas dos sistemas apresentados nesta disserta??o
44
Morelle, Alessandra Menezes. "Detec??o de mamaglobina (HMAM) e ant?geno carcinoembri?nico (CEA) por RT-PCR em linfonodo, sangue perif?rico e medula ?ssea de mulheres submetidas a tratamento cir?rgico de c?ncer de mama." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2005. http://tede2.pucrs.br/tede2/handle/tede/1648.
Full textAbstract:
Made available in DSpace on 2015-04-14T13:35:19Z (GMT). No. of bitstreams: 1
433622.pdf: 643872 bytes, checksum: 21a222dc14b2e23cb481fc34a34b6bfc (MD5)
Previous issue date: 2005-08-09Racional: A apropriada indica??o de terapia adjuvante no c?ncer de mama est? atualmente embasada em fatores progn?sticos cl?nico-patol?gicos. A busca de fatores mais precisos e confi?veis para a identifica??o de pacientes em que a indica??o de tratamento complementar ? imprescind?vel, ? de grande import?ncia nos dias atuais. A mamaglobina (hMAM) e o ant?geno carcinoembri?nico (CEA) t?m sido alvos de estudos como marcadores de micromet?stases de c?ncer de mama. O objetivo principal deste estudo foi avaliar a capacidade de detec??o de micromet?stases de c?ncer de mama pelo m?todo de RT-PCR para hMAM e CEA.M?todos: Quarenta e nove pacientes com c?ncer de mama EC I a III, foram avaliadas no momento da cirurgia de mama com coleta de amostras de tecido mam?rio normal, tumor, linfonodo, sangue e medula ?ssea. Realizou-se RT-PCR para CEA e hMAM nos respectivos tecidos.Resultados: A maioria dos tumores expressaram CEA (37/ 44 casos) e hMAM (40/44 casos). Seis pacientes (27.3%) com histologia negativa nos linfonodos axilares expressaram CEA nos mesmos e 2 (8,7%) pacientes com esta situa??o expressaram hMAM nos linfonodos.Duas (5,7%) pacientes expressaram CEA no sangue e cinco (13,9%) expressaram hMAM. A medula ?ssea teve express?o de CEA em 3 (14,3%) pacientes e de hMAM em 5 (23,8%).Conclus?o: A t?cnica de RT-PCR ? capaz de detectar transcritos de CEA e hMAM em pacientes com c?ncer de mama. O significado cl?nico deste achado ainda deve ser esclarecido. A detec??o em linfonodos para estes marcadores parece estar mais fortemente associada ao progn?stico que a detec??o em sangue e medula ?ssea.
45
Paiva, Luiz Ant?nio Nascimento de. "Detec??o de ?reas degradadas na sub-bacia hidrogr?fica do rio Tapero?/PB, utilizando par?metros f?sicos dos sensores MODIS/terra e TM/landsat." Universidade Federal do Rio Grande do Norte, 2008. http://repositorio.ufrn.br:8080/jspui/handle/123456789/18896.
Full textAbstract:
Made available in DSpace on 2015-03-13T17:10:38Z (GMT). No. of bitstreams: 1
LuizANP_DISSERT_1-80.pdf: 5205000 bytes, checksum: a0528798e1c98d3a73a67178f824421d (MD5)
Previous issue date: 2008-11-12This study includes the results of the analysis of areas susceptible to degradation by remote sensing in semi-arid region, which is a matter of concern and affects the whole population and the catalyst of this process occurs by the deforestation of the savanna and improper practices by the use of soil. The objective of this research is to use biophysical parameters of the MODIS / Terra and images TM/Landsat-5 to determine areas susceptible to degradation in semi-arid Paraiba. The study area is located in the central interior of Para?ba, in the sub-basin of the River Tapero?, with average annual rainfall below 400 mm and average annual temperature of 28 ? C. To draw up the map of vegetation were used TM/Landsat-5 images, specifically, the composition 5R4G3B colored, commonly used for mapping land use. This map was produced by unsupervised classification by maximum likelihood. The legend corresponds to the following targets: savanna vegetation sparse and dense, riparian vegetation and exposed soil. The biophysical parameters used in the MODIS were emissivity, albedo and vegetation index for NDVI (NDVI). The GIS computer programs used were Modis Reprojections Tools and System Information Processing Georeferenced (SPRING), which was set up and worked the bank of information from sensors MODIS and TM and ArcGIS software for making maps more customizable. Initially, we evaluated the behavior of the vegetation emissivity by adapting equation Bastiaanssen on NDVI for spatialize emissivity and observe changes during the year 2006. The albedo was used to view your percentage of increase in the periods December 2003 and 2004. The image sensor of Landsat TM were used for the month of December 2005, according to the availability of images and in periods of low emissivity. For these applications were made in language programs for GIS Algebraic Space (LEGAL), which is a routine programming SPRING, which allows you to perform various types of algebras of spatial data and maps. For the detection of areas susceptible to environmental degradation took into account the behavior of the emissivity of the savanna that showed seasonal coinciding with the rainy season, reaching a maximum emissivity in the months April to July and in the remaining months of a low emissivity . With the images of the albedo of December 2003 and 2004, it was verified the percentage increase, which allowed the generation of two distinct classes: areas with increased variation percentage of 1 to 11.6% and the percentage change in areas with less than 1 % albedo. It was then possible to generate the map of susceptibility to environmental degradation, with the intersection of the class of exposed soil with varying percentage of the albedo, resulting in classes susceptibility to environmental degradation
A presente pesquisa compreende os resultados de an?lises de ?reas suscet?veis a degrada??o ambiental por sensoriamento remoto no semi-?rido nordestino, o que ? um fato preocupante e atinge toda popula??o e o efeito catalisador desse processo ocorre pelo desmatamento da caatinga e por pr?ticas inadequadas do uso do solo. Assim, o objetivo desta pesquisa ? utilizar par?metros biof?sicos do sensor MODIS/Terra e as imagens TM/Landsat-5 para determinar as ?reas suscept?veis ao processo de degrada??o no semi-?rido para?bano. A ?rea de estudo localiza-se no sert?o central da Para?ba, na Sub-bacia do Rio Tapero?, com m?dias anuais de precipita??o inferiores a 400 mm e temperatura m?dia anual de 28?C. Para a elabora??o do mapa de cobertura vegetal foram utilizadas as imagens TM/Landsat- 5, especificamente, a composi??o colorida 5R4G3B, mais utilizada para mapeamento do uso do solo. Este mapa foi confeccionado pela Classifica??o Supervisonada por M?xima Verossimilhan?a. A legenda corresponde aos seguintes alvos: vegeta??o de caatinga densa e esparsa; vegeta??o ciliar e solo exposto. Os par?metros biof?sicos utilizados do sensor MODIS foram: emissividade, albedo e ?ndice de Vegeta??o por Diferen?a Normalizada (NDVI). Os programas computacionais de geoprocessamento utilizados foram o Modis Reprojections Tools e o Sistema de Processamento de Informa??es Georreferenciadas (SPRING), no qual foi montado e trabalhado o banco de informa??es dos sensores MODIS e TM e o software ArcGIS para a confec??o de cartas mais customiz?veis. Inicialmente, avaliou o comportamento da vegeta??o pela emissividade por meio da adapta??o da equa??o de Bastiaanssen sobre o NDVI para espacializar a emissividade e observar as altera??es durante o ano de 2006. O albedo foi utilizado para visualizar o seu percentual de aumento nos per?odos de dezembro de 2003 e 2004. As imagens do sensor TM/Landsat usadas foram do m?s de dezembro de 2005, de acordo com a disponibilidade das imagens e no per?odo de menor emissividade. Para estas aplica??es foram feitos programas em Linguagem Espacial para Geoprocessamento Alg?brico (LEGAL), que ? uma rotina de programa??o do SPRING, a qual permite realizar v?rios tipos de ?lgebras de dados e mapas espaciais. Para a detec??o de ?reas suscept?veis ao processo de degrada??o ambiental levou-se em considera??o o comportamento da emissividade da caatinga que se mostrou sazonal coincidindo com o per?odo chuvoso atingindo o m?ximo de emissividade nos meses de abril a julho e nos restantes dos meses uma baixa emissividade. Com as imagens do albedo de dezembro de 2003 e 2004, foi verificado o seu aumento percentual, o que possibilitou a gera??o de duas classes distintas: ?reas com aumento da varia??o percentual de 1 a 11,6% e ?reas com varia??o percentual inferior a 1% do albedo. A partir da? foi poss?vel gerar o mapa de susceptibilidade ? degrada??o ambiental, com o cruzamento da classe de solo exposto com a varia??o percentual do albedo, resultando em classes susceptibilidade ? degrada??o ambiental
46
Corr?a, Ricardo Augusto Moreira de Souza. "Otimiza??o dos par?metros de eletropolimeriza??o do ?cido 4-hidroxifenilac?tico para utiliza??o no desenvolvimento de genossensores aplicados na detec??o de Mycobacterium tuberculosis." UFVJM, 2015. http://acervo.ufvjm.edu.br/jspui/handle/1/810.
Full textAbstract:
?rea de concentra??o: Qu?mica Anal?tica.Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-06T19:01:54Z No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) ricardo_augusto_m_souza_correa.pdf: 3203874 bytes, checksum: 613643e6fe67082b3f47a7d8e0528aaf (MD5)
Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-07T11:53:33Z (GMT) No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) ricardo_augusto_m_souza_correa.pdf: 3203874 bytes, checksum: 613643e6fe67082b3f47a7d8e0528aaf (MD5)
Made available in DSpace on 2016-01-07T11:53:33Z (GMT). No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) ricardo_augusto_m_souza_correa.pdf: 3203874 bytes, checksum: 613643e6fe67082b3f47a7d8e0528aaf (MD5) Previous issue date: 2015
Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG)
Foi otimizada a eletropolimeriza??o do ?cido 4-hidroxifenilac?tico (4-HFA), visando sua aplica??o como plataforma funcionalizada para imobiliza??o de biomol?culas, para o desenvolvimento de genossensores. Foi utilizado o mon?mero 4-HFA, e por meio deste a eletrogera??o foi conduzida sobre a superf?cie do eletrodo de grafite (EG), utilizando-se a t?cnica de voltametria c?clica na faixa de +0,0 a +1,20 V, onde foram investigados dois par?metros: n?mero de ciclos de potencial aplicado e velocidade de varredura utilizada. Associado a este estudo, foi investigado a imobiliza??o de pequenos fragmentos de DNA (oligonucleot?deos), observando a atua??o da plataforma funcionalizada na resposta do biossensor para detec??o dos oligonucleot?deos, bem como avalia??o do reconhecimento do evento de hibridiza??o com o alvo complementar. Observou-se que o filme polim?rico formado apresentou um par redox na regi?o +0,53/+0,38 V e o aumento do n?mero de ciclos gera plataformas mais eletroativas devido a maior quantidade de material adsorvido, por outro lado, a diminui??o da velocidade de varredura gera plataformas mais eletroativas devido a ocorr?ncia do acoplamento mais organizado. Medidas de espectroscopia de imped?ncia eletroqu?mica (EIE) mostraram maior resist?ncia do filme para os eletrodos modificados com maior n?mero de ciclos, bem como para os eletrodos modificados com maiores velocidades de varredura. Imagens de microscopia eletr?nica de varredura (MEV) mostraram que em todos os casos n?o h? total recobrimento da superf?cie do EG e corroboraram com os demais resultados encontrados. As imagens de MEV demonstraram que diferentes ciclagens n?o influenciam na morfologia do filme formado, mas sim na quantidade de material adsorvido. Por outro lado, as imagens tamb?m mostraram que as diferentes velocidades de varredura geram filmes com morfologias distintas. A plataforma EG/poli(4-HFA) mostrou-se eficiente e sens?vel para a imobiliza??o de oligonucleot?deos, bem como para o evento de hibridiza??o com o oligonucleot?deo complementar. O eletrodo que apresentou as melhores respostas para imobiliza??o das ssDNAs estudadas e detec??o dos respectivos alvos complementares foi o eletrodo modificado com 100 ciclos de potencial na velocidade de varredura de 75 mV/s, uma vez que mostrou maiores amplitudes nos valores de corrente de pico. A constru??o do genossensor para detec??o do bacilo Mycobacterium tuberculosis confirmou os demais resultados acerca da efici?ncia da plataforma EG/poli(4-HFA), uma vez que a mesma demonstrou excelente sensibilidade ao utilizar o Azul de Metileno (AM) como intercalador. O genossensor desenvolvido apresentou um excelente limite de detec??o de 0,16 nmol, operando com volumes baix?ssimos de solu??o, sendo estes 15 ?L de sonda MYC e 10 ?L de alvo MYC. Foi poss?vel desenvolver o dispositivo e ainda otimizar v?rios par?metros de adsor??o da sonda e hibridiza??o do alvo, o que ocasionou uma melhoria da diminui??o do sinal de redu??o do AM de 14% para 34%. Em adi??o, estudos com o interferente MYC-NE demonstraram que o genossensor possui seletividade satisfat?ria, uma vez que a hibridiza??o com o interferente acarretou em diminui??o do sinal 46% inferior quando comparado ao alvo espec?fico.
Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015.
ABSTRACT Aiming its application as functionalized platform for biomolecules immobilization, it was optimized the electropolymerization of 4-hydroxyphenylacetic acid (4-HPA), for genosensors development. The monomer 4-HPA was used and, by means of it the electrogeneration was carried out on the surface of the graphite electrode (GE), using cyclic voltammetry on the range of +0,0 to +1,20 V, in which were investigated two parameters: number of cycles of applied potential and scan rate used. Associated to this work it was investigated small DNA fragments (oligonucleotides), observing the performance of the functionalized platform in biosensor response to detection of oligonucleotides, as well as evaluation hybridization event recognition with the complementary target. It was observed that the polymeric film showed a redox couple in region +0,53/+0,38 V and an increase of the number of cycles produces more electroactive platforms due to greater amount of adsorbed material. On the other hand, a decrease in scan rate produces more electroactive platforms due to the occurrence of more organized coupling. Electrochemical Impedance Spectroscopy (EIS) measurements showed higher film resistance to the modified electrodes with more number of cycles, as well as for the modified electrode with higher scan rate. Images of scanning electron microscopy (SEM) have shown that in all cases there is no complete coverage of the GE surface and corroborated with the other results found. SEM images have shown that the number of cycles does not influence the morphology of the formed film, but the amount of the adsorbed material. On the other hand, images also have shown that different scan rates produce films with distinct morphologies. GE/poly(4-HPA) platform have shown to be sensitive and efficient to oligonucleotide immobilization, as well as for hybridization event with the complementary oligonucleotide. The electrode that showed the best responses to the immolization of the studied ssDNA and the respective complementary target detection was the electrode modified with 100 potential cycles in the scan rate of 75 mV/s since it has shown higher amplitudes at peak current values. Genosensor construction for Mycobacterium tuberculosis bacillus detection confirmed the results about the GE/poly(4-HPA) platform efficiency, since it has shown excellent sensitivity when using Methilene Blue (MB) as intercalator. The designed biosensor has shown an excellent limit detection of 0,16 nmol, operating with very low solution volumes these being 15 ?L of MYC probe and 10 ?L MYC target. It was possible develop the device and even optimize several probe adsorption parameters and target hybridization which led to an improvement in decrease of the MB reduction signal from 14% to 34%. In addition, studies with the interfering MYC-NE has shown that the genosensor has satisfactory selectivity since the hybridization with the interfering resulted in a signal decrease 46% lower when compared to the specific target.
47
Hejl, Zdeněk. "Rekonstrukce 3D scény z obrazových dat." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2012. http://www.nusl.cz/ntk/nusl-236495.
Full textAbstract:
This thesis describes methods of reconstruction of 3D scenes from photographs and videos using the Structure from motion approach. A new software capable of automatic reconstruction of point clouds and polygonal models from common images and videos was implemented based on these methods. The software uses variety of existing and custom solutions and clearly links them into one easily executable application. The reconstruction consists of feature point detection, pairwise matching, Bundle adjustment, stereoscopic algorithms and polygon model creation from point cloud using PCL library. Program is based on Bundler and PMVS. Poisson surface reconstruction algorithm, as well as simple triangulation and own reconstruction method based on plane segmentation were used for polygonal model creation.
48
Horký, Vladimír. "Rozpoznávání ručně psaného písma pomocí neuronových sítí." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2012. http://www.nusl.cz/ntk/nusl-236462.
Full textAbstract:
Neural networks with algorithm back-propagation will be presented in this work. Theoretical background of the algorithm will be explained. The problems with training neural nets will be solving there. The work discuss some techniques of image preprocessing and image extraction features, which is one of main part in classification. Some part of work discuss few experiments with neural nets with chosen image features.
49
Stanek, Timotej. "Automatické shlukování regulárních výrazů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2011. http://www.nusl.cz/ntk/nusl-235531.
Full textAbstract:
This project is about security of computer networks using Intrusion Detection Systems. IDS contain rules for detection expressed with regular expressions, which are for detection represented by finite-state automata. The complexity of this detection with non-deterministic and deterministic finite-state automata is explained. This complexity can be reduced with help of regular expressions grouping. Grouping algorithm and approaches for speedup and improvement are introduced. One of the approches is Genetic algorithm, which can work real-time. Finally Random search algorithm for grouping of regular expressions is presented. Experiment results with these approches are shown and compared between each other.
50
Piton, Nicolas. "Optimisation de la prise en charge diagnostique, pronostique et théranostique des carcinomes broncho-pulmonaires humains : des techniques d’imagerie in vivo à la biologie moléculaire. Ligation -dependent RT-PCR : a new specific and low-cost technique to detect ALK, ROS and RET rearrangements in lung adenocarcinoma A new assay for detection of theranostic gene translocations and MET exon 14 skipping in thoracic oncology. One-year perspective routine LD-RT-PCR in 413 newly diagnosed lung tumors STK11 mutations are associated with lower PDL1 expression in lung adenocarcinoma BRAF V600E mutation is not always present as expected ! A case report of lung and thyroid carcinomas A novel method for in vivo imaging of solitary lung nodules using navigational bronchoscopy and confocal laser microendoscopy." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR119.
Full textAbstract:
Le carcinome pulmonaire est une affection grave et fréquente dont la prise en charge a été bouleversée ces dernières années, tant sur le plan diagnostique que pronostique ou « théranostique » avec l’avènement des « thérapies ciblées ». Ces dernières permettent une nette amélioration de la survie et du confort des patients éligibles, mais ne sont pas sans compliquer le travail médical, depuis le diagnostic de la maladie jusqu’au suivi régulier du patient, sans oublier le choix des traitements ou les problèmes techniques posés par la multiplication arborescente des altérations moléculaires à rechercher à partir d’un tissu tumoral souvent peu abondant dans ce contexte particulier de l’oncologie thoracique. Ce travail de thèse collige 5 travaux de recherche selon deux angles d’approche : les marqueurs moléculaires pronostiques et « théranostiques » du cancer pulmonaire, et les procédures de diagnostic in vivo de cette pathologie. Le premier axe comporte 4 articles. Les deux premiers concernent l’évaluation d’une nouvelle technique moléculaire, la LD-RT-PCR, dans la détection des translocation géniques du cancer pulmonaire : la première étude est une étude de faisabilité, la deuxième est un travail de validation. Le troisième article explore l’association entre la présence d’une mutation STK11 dans les carcinomes pulmonaires et l’expression de PDL1. Enfin, le quatrième article est une étude de cas illustrant l’importance de l’approche morphologique du cancer pulmonaire. Le second axe est représenté par un travail comparant une technique d’imagerie in vivo par voie endoscopique utilisant la micro-endoscopie confocale par laser avec l’approche microscopique conventionnelleLung cancer is a serious and frequent condition for which the management strategies have been dramatically modified in recent years, from a diagnostic, prognostic and “theranostic” perspective, most notably with the introduction of “targeted therapies”. The latter have demonstrated dramatic improvement in both quality of life and survival rates of eligible patients, yet consequently highlight new complications in diagnosis, treatment options or technical considerations which can be attributed to the growing number of molecular alterations to be detected from limited tissue samples frequently encountered in thoracic oncology. This work combines 5 different research papers from 2 different angles: prognostic and “theranostic” molecular markers of lung cancer, as well as in vivo diagnostic procedures of lung cancer. The first angle encompasses 4 articles. The first two evaluate a new molecular technique, LD-RT-PCR, to detect gene translocation in lung cancer. The third article explores the association between STK11 mutations in lung cancer and the expression of PDL1. Finally, the fourth article is a case report illustrating the importance of a morphological approach to lung cancer. The second angle compares in vivo imaging techniques by endoscopy using confocal laser microendoscopy alongside a conventional microscopic approach