Journal articles: 'PCR diagnostic'

1

Bianchi, A. "Diagnostic par PCR des infections à Chlamydia trachomatis." Revue Française des Laboratoires 1997, no. 292 (April 1997): 166–67. http://dx.doi.org/10.1016/s0338-9898(97)80069-8.

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Dupont, Damien, Céline Dupieux, Pascal Gaucherand, and Martine Wallon. "Diagnostic fortuit de Trichomonas vaginalis par PCR panfongique." Journal de Mycologie Médicale 27, no. 3 (September 2017): e40. http://dx.doi.org/10.1016/j.mycmed.2017.04.093.

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Zamfir, O., H. Yera, T. Bourcier, L. Batellier, J. Dupouy-Camet, C. Tourte-Schaeffer, and C. Chaumeil. "Diagnostic par PCR des kératites à Acanthamoeba spp." Journal Français d'Ophtalmologie 29, no. 9 (November 2006): 1034–40. http://dx.doi.org/10.1016/s0181-5512(06)73892-x.

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Dorson, O., and F. Doucet-Populaire. "Le diagnostic biologique de la coqueluche par PCR." Journal de Pédiatrie et de Puériculture 12, no. 8 (December 1999): 474–79. http://dx.doi.org/10.1016/s0987-7983(99)80138-8.

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Volkov, A. N., L. V. Nacheva, and Yu V. Zakharova. "Molecular genetic techniques in current biomedical research. Part II: PCR applications in diagnostics of human infectious diseases." Fundamental and Clinical Medicine 6, no. 1 (March 29, 2021): 77–85. http://dx.doi.org/10.23946/2500-0764-2021-6-1-77-85.

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Polymerase chain reaction (PCR)-based diagnostics is currently established as a gold standard for the detection of microorganisms. The features of PCR include rapid amplification of DNA and RNA as well as high sensitivity and specificity. In contrast to diagnostic microbiology, PCR diagnostics does not require preliminary culture of the microorganisms for their identification, reducing both time and costs of the diagnostic procedure. The lecture discusses the molecular basis behind the modern technical solutions for the PCR diagnostics of human infectious diseases including multiplex and reverse transcription PCR. We describe the principles of qualitative and quantitative PCR-based detection of pathogens in biological samples and provide the examples of PCR application for solving specific diagnostic scenarios. The lecture is primarily designed for students of biomedical specialties and healthcare professionals using molecular genetic techniques in their practice.

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Mirmajlessi, Seyed Mahyar, Maria Jennifer Sjölund, Marika Mänd, Marianne Loiseau, Vincenza Ilardi, Geert Haesaert, Reet Karise, Richard Alexander Gottsberger, Jason Sumner-Kalkun, and Assunta Bertaccini. "PCR-based diagnostic methods for ‘Candidatus Liberibacter solanacearum’ – Review." Plant Protection Science 55, No. 4 (September 13, 2019): 228–41. http://dx.doi.org/10.17221/145/2018-pps.

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‘Candidatus Liberibacter solanacearum’ is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several databases were searched from the inception of the relevant literature up to August 2018. This review identified 53 studies that met all the inclusion criteria. The performance of the different methods was also compared, however due to data heterogeneity and insufficient evidence on the sensitivity of all assays used, a meta-analysis of the data was not possible. Nonetheless, the review indicates that the rtPCR designed to the 16S ribosomal RNA gene can be routinely employed as a fast, cost-effective, and reliable detection technique in diagnostic laboratories.

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Emile, Carole. "Diagnostic rapide des maladies infectieuses par PCR multiplexe syndromique." Option/Bio 30, no. 599-600 (June 2019): 16–17. http://dx.doi.org/10.1016/s0992-5945(19)30235-1.

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Prevel, A. "Diagnostic direct par PCR des infections à Bordeteila pertussis." Archives de Pédiatrie 2, no. 12 (December 1995): 1227–28. http://dx.doi.org/10.1016/0929-693x(95)90066-c.

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9

Castellani, Rudy J. "Diagnostic Challenges in Surgical Neuropathology." Pathology Case Reviews 18, no. 6 (2013): 243. http://dx.doi.org/10.1097/pcr.0000000000000012.

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Ioffe, Olga. "Diagnostic Problems in Breast Pathology." Pathology Case Reviews 14, no. 4 (July 2009): 127–28. http://dx.doi.org/10.1097/pcr.0b013e3181b911d5.

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Ioffe, Olga B. "Rare But Important Diagnostic Entities." AJSP: Reviews & Reports 25, no. 6 (2020): 265. http://dx.doi.org/10.1097/pcr.0000000000000418.

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Batra, Vineeta V., Michael Mines, and Fausto J. Rodriguez. "Diagnostic Review of Neurofibromatosis Type 1." Pathology Case Reviews 19, no. 2 (2014): 57–65. http://dx.doi.org/10.1097/pcr.0000000000000025.

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Jennette, J. Charles. "Diagnostic Classification of Vasculitis: An Overview." Pathology Case Reviews 12, no. 5 (September 2007): 177–78. http://dx.doi.org/10.1097/pcr.0b013e3181557dab.

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Pritchard, Colin C., and Matthew M. Yeh. "Pathology and Diagnostic Pitfalls of Cholangiocarcinoma." Pathology Case Reviews 14, no. 1 (January 2009): 28–33. http://dx.doi.org/10.1097/pcr.0b013e31819c4b66.

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15

Montone, Kathleen T. "Diagnostic Considerations in Infectious Disease Pathology." Pathology Case Reviews 16, no. 6 (November 2011): 223. http://dx.doi.org/10.1097/pcr.0b013e31823c95cd.

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16

Hoegh, A. M., H. Westh, and G. Lisby. "Basics of diagnostic PCR assay design." Journal of Clinical Virology 36 (January 2006): S28—S29. http://dx.doi.org/10.1016/s1386-6532(06)80805-x.

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Gräser, Yvonne, Viktor Czaika, and Torsten Ohst. "Diagnostic PCR of dermatophytes - an overview." JDDG: Journal der Deutschen Dermatologischen Gesellschaft 10, no. 10 (May 29, 2012): 721–25. http://dx.doi.org/10.1111/j.1610-0387.2012.07964.x.

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18

Boelaert, M., and J. C. Dujardin. "Diagnostic PCR with Leishmania donovani specificity." Tropical Medicine and International Health 4, no. 11 (November 1999): 789. http://dx.doi.org/10.1046/j.1365-3156.1999.00487.x.

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19

Krause, A., S. Priem, and G. R. Burmester. "Borrelien-PCR: Diagnostic Problems Constantly Remain?" Zeitschrift f�r Rheumatologie 57, no. 2 (April 28, 1998): 79–81. http://dx.doi.org/10.1007/s003930050063.

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20

Mangayarkarasi, V., Sneka P, Sujith R, and Jayaprakash Jayaprakash. "Ergonomic Diagnostic Tool based on Chip Mini RT-PCR for Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis." Journal of Pure and Applied Microbiology 13, no. 2 (June 30, 2019): 1185–90. http://dx.doi.org/10.22207/jpam.13.2.58.

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21

PERUCHON, Sandrine, Sylvie HENAULT, Christiane MENDY, and Josée VAISSAIRE. "Diagnostic de la tularémie par amplification génique in vitro (PCR)." Bulletin de l'Académie vétérinaire de France, no. 1 (2006): 165. http://dx.doi.org/10.4267/2042/47829.

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22

De Charry, F., M. Rabodonirina, C. Fuhrmann, C. Sebban, and H. Ghesquieres. "Diagnostic par PCR de la pneumocystose pulmonaire en onco-hématologie." La Revue de Médecine Interne 36 (December 2015): A78. http://dx.doi.org/10.1016/j.revmed.2015.10.304.

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23

Dell'isola, B., O. Gaillot, P. Amouriaux, G. Baranton, I. Saint Girons, M. Simonet, and J. C. Valcke. "Diagnostic biologique de la leptospirose par PCR (polymerase chain reaction)." La Revue de Médecine Interne 14, no. 8 (January 1993): 805–6. http://dx.doi.org/10.1016/s0248-8663(05)81430-4.

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24

Bustin, Stephen A., and Reinhold Mueller. "Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis." Clinical Science 109, no. 4 (September 23, 2005): 365–79. http://dx.doi.org/10.1042/cs20050086.

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qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.

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25

Lee, Si Won, Yong-Gil Shin, Jin-Young Lee, Young-Suk Kim, Mi Hee Yang, and In-Cheol Choi. "Development of a diagnostic system to detect potato virus T using RT-PCR and nested PCR." CNU Journal of Agricultural Science 42, no. 2 (June 30, 2015): 99–103. http://dx.doi.org/10.7744/cnujas.2015.42.2.099.

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26

Morgan, U. "The development of diagnostic PCR primers for Cryptosporidium using RAPD-PCR." Molecular and Biochemical Parasitology 77, no. 1 (April 1996): 103–8. http://dx.doi.org/10.1016/0166-6851(96)02577-7.

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27

Fuchs, Lass-Flörl, and Posch. "Diagnostic Performance of a Novel Multiplex PCR Assay for Candidemia among ICU Patients." Journal of Fungi 5, no. 3 (September 17, 2019): 86. http://dx.doi.org/10.3390/jof5030086.

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Candidemia poses a major threat to ICU patients and is routinely diagnosed by blood culture, which is known for its low sensitivity and long turnaround times. We compared the performance of a novel, Candida-specific multiplex real-time PCR assay (Fungiplex® Candida IVD Real-Time PCR Kit) with blood culture and another established diagnostic real-time PCR assay (LightCycler SeptiFast Test) with respect to Candida detection from whole blood samples. Clinical samples from 58 patients were analyzed by standard blood culture (BC) and simultaneously tested with the Fungiplex Candida PCR (FP) and the SeptiFast test (SF) for molecular detection of Candida spp. Compared to BC, the FP test showed high diagnostic power, with a sensitivity of 100% and a specificity of 94.1%. Overall diagnostic accuracy reached 94.6%. Using SF, we found a sensitivity of 60%, a specificity of 96.1%, and an overall diagnostic accuracy of 92.9%. The Fungiplex Candida PCR has shown good sensitivity and specificity on clinical samples of high-risk patients for direct detection of Candida species in whole blood samples. Together with conventional diagnostics (BC and antigen testing), this new multiplex PCR assay may contribute to a rapid and accurate diagnosis of candidiasis.

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28

Hecht, Leonie-Sophie, Angeles Jurado-Jimenez, Markus Hess, Hussein El Halas, Gregor Bochenek, Hala Mohammed, Fahd Alzahrani, et al. "Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0." Future Microbiology 14, no. 11 (July 2019): 941–48. http://dx.doi.org/10.2217/fmb-2019-0067.

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Aim: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. Material & methods: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. Results: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. Conclusion: The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.

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29

Legesse, Teklu, and Olga B. Ioffe. "Diagnostic Immunohistochemistry of Epithelial Lesions of the Breast." AJSP: Review and Reports 21, no. 1 (2016): 11–15. http://dx.doi.org/10.1097/pcr.0000000000000126.

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30

Lennerz, Jochen KM, Jeffrey S. Crippin, and Elizabeth M. Brunt. "Diagnostic Considerations of Nodules in the Cirrhotic Liver." Pathology Case Reviews 14, no. 1 (January 2009): 3–12. http://dx.doi.org/10.1097/pcr.0b013e31819c4238.

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31

Behtaj, Mohadese, Muhammad Omar Hakim, Jaylou Velez Torres, Andrew E. Rosenberg, and Elizabeth Anne Montgomery. "Common Differential Diagnostic Issues in Soft Tissue Pathology." AJSP: Reviews and Reports 26, no. 1 (January 2021): 2–16. http://dx.doi.org/10.1097/pcr.0000000000000420.

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32

Hakim, Muhammad O., Mohadese Behtaj, Jaylou Velez Torres, Elizabeth A. Montgomery, and Andrew E. Rosenberg. "Common Differential Diagnostic Issues in Bone Tumor Pathology." AJSP: Reviews and Reports 26, no. 1 (January 2021): 17–34. http://dx.doi.org/10.1097/pcr.0000000000000419.

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33

Benabdellah, A., N. Ghozali, S. Tigaud, and H. Salord. "G-13 Analyse du diagnostic de la peste par RT-PCR." Médecine et Maladies Infectieuses 34 (June 2004): S165. http://dx.doi.org/10.1016/s0399-077x(04)90236-3.

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34

Cambien, G., A. Lariviere, C. Chessa, L. Marco, J. Guihenneuc, A. Bousseau, C. Laland, O. Castel, N. Leveque, and S. Thevenot. "Diagnostic des virus respiratoires par PCR-multiplex aux urgences : étude d’impact." Médecine et Maladies Infectieuses 50, no. 6 (September 2020): S10. http://dx.doi.org/10.1016/j.medmal.2020.06.026.

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35

Fabre, R., A. Berry, and J. F. Magnaval. "Diagnostic du paludisme d’importation par PCR multiplex compétitive sur LightCycler™." Pathologie Biologie 51, no. 1 (February 2003): 44–46. http://dx.doi.org/10.1016/s0369-8114(02)00319-x.

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36

Dell'isola, B., I. Saint Girons, P. Amouriaux, G. Baranton, A. M. El Maleh, M. Simonet, and J. C. Valcke. "Diagnostic biologique précoce de la leptospirose par PCR (polymerase chain reaction)." La Revue de Médecine Interne 14, no. 6 (June 1993): 578. http://dx.doi.org/10.1016/s0248-8663(05)80512-0.

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37

Kugeler, Kiersten J., Nikos Gurfield, Jean G. Creek, Kerry S. Mahoney, Jessica L. Versage, and Jeannine M. Petersen. "Discrimination between Francisella tularensis and Francisella-Like Endosymbionts when Screening Ticks by PCR." Applied and Environmental Microbiology 71, no. 11 (November 2005): 7594–97. http://dx.doi.org/10.1128/aem.71.11.7594-7597.2005.

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ABSTRACT The presence of Francisella-like endosymbionts in tick species known to transmit tularemia poses a potential diagnostic problem for laboratories that screen tick samples by PCR for Francisella tularensis. Tick samples initially considered positive for F. tularensis based on standard 16S rRNA gene PCR were found to be positive only for Francisella-like endosymbionts using a multitarget F. tularensis TaqMan assay (ISFtu2, tul4, and iglC) and 16S rRNA gene sequencing. Specificity of PCR-based diagnostics for F. tularensis should be carefully evaluated with appropriate specimen types prior to diagnostic use.

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38

Brillowska-DaąBrowska, Anna, Aleksandra Świerkowska, Ditte Marie Lindhardt Saunte, and Maiken Cavling Arendrup. "Diagnostic PCR tests forMicrosporum audouinii, M. canisandTrichophytoninfections." Medical Mycology 48, no. 3 (May 2010): 486–90. http://dx.doi.org/10.3109/13693780903312454.

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39

Bricker, Betsy J. "PCR as a diagnostic tool for brucellosis." Veterinary Microbiology 90, no. 1-4 (December 2002): 435–46. http://dx.doi.org/10.1016/s0378-1135(02)00228-6.

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40

Vinner, Lasse, and Anders Fomsgaard. "Inactivation of orthopoxvirus for diagnostic PCR analysis." Journal of Virological Methods 146, no. 1-2 (December 2007): 401–4. http://dx.doi.org/10.1016/j.jviromet.2007.07.025.

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41

Kuno, Goro. "Universal diagnostic RT-PCR protocol for arboviruses." Journal of Virological Methods 72, no. 1 (May 1998): 27–41. http://dx.doi.org/10.1016/s0166-0934(98)00003-2.

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42

Doris, Kathryn, Sarah Kempster, Cristina Santirso-Margaretto, Graham Prescott, Clare Morris, Neil Almond, and Rob Anderson. "Controlling the quality of diagnostic PCR assays." Journal of Clinical Virology 82 (September 2016): S105—S106. http://dx.doi.org/10.1016/j.jcv.2016.08.211.

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43

Williams, M. L., and M. L. Lawrence. "Verification of anEdwardsiella ictaluri-specific diagnostic PCR." Letters in Applied Microbiology 50, no. 2 (February 2010): 153–57. http://dx.doi.org/10.1111/j.1472-765x.2009.02770.x.

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44

Sharma, Narotam, Veena Sharma, Prem Raj Singh, Shivani Sailwal, Rajeev S. Kushwaha, Rajesh K. Singh, Satish C. Nautiyal, Pankaj Mishra, Tariq Masood, and R. K. Singh. "Diagnostic Value of PCR in Genitourinary Tuberculosis." Indian Journal of Clinical Biochemistry 28, no. 3 (December 6, 2012): 305–8. http://dx.doi.org/10.1007/s12291-012-0279-7.

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45

Odze, Robert D. "Diagnostic Approach to Biopsies of the Gastroesophageal Junction (“Cardia”)." Pathology Case Reviews 13, no. 5 (September 2008): 172–78. http://dx.doi.org/10.1097/pcr.0b013e318186ab00.

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46

Shah, Sejal S., Katrina N. Glazebrook, and Carol Reynolds. "Diagnostic Consideration of Spindle Cell Proliferations in the Breast." Pathology Case Reviews 14, no. 4 (July 2009): 172–76. http://dx.doi.org/10.1097/pcr.0b013e3181b320ef.

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47

Chen, Yi-Hua. "Diagnostic Challenges in Bone Marrow Lymphoid and Histiocytic Infiltrates." Pathology Case Reviews 17, no. 3 (2012): 99–100. http://dx.doi.org/10.1097/pcr.0b013e31825c7042.

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48

Miller, Kennon, Susan M. Harrington, and Gary W. Procop. "Acid-fast Smear and Histopathology Results Provide Guidance for the Appropriate Use of Broad-Range Polymerase Chain Reaction and Sequencing for Mycobacteria." Archives of Pathology & Laboratory Medicine 139, no. 8 (January 9, 2015): 1020–23. http://dx.doi.org/10.5858/arpa.2013-0705-oa.

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Context New molecular diagnostic tests are attractive because of the potential they hold for improving diagnostics in microbiology. The value of these tests, which is often assumed, should be investigated to determine the best use of these potentially powerful tools. Objective To investigate the usefulness of broad-range polymerase chain reaction (PCR), followed by sequencing, in mycobacterial infections. Design We reviewed the test performance of acid-fast bacilli (AFB) PCR and traditional diagnostic methods (histopathology, AFB smear, and culture). We assessed the diagnostic effect and cost of the unrestricted ordering of broad-range PCR for the detection and identification of mycobacteria in clinical specimens. Results The AFB PCR was less sensitive than culture and histopathology and was less specific than culture, AFB smear, and histopathology. During 18 months, $93 063 was spent on 183 patient specimens for broad-range PCR and DNA sequencing for mycobacteria to confirm one culture-proven Mycobacterium tuberculosis infection that was also known to be positive by AFB smear and histopathology. In this cohort, there was a false-negative AFB PCR for M tuberculosis and a false-positive AFB PCR for Mycobacterium lentiflavum. Conclusion Testing of AFB smear–negative specimens from patients without an inflammatory response supportive of a mycobacterial infection is costly and has not been proven to improve patient care. Traditional diagnostics (histopathology, AFB smear, and culture) should remain the primary methods for the detection of mycobacteria in clinical specimens.

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Marih, Latifa, and Mustapha Sodqi. "Diagnostic de l’infection par le SARS-CoV2." Batna Journal of Medical Sciences (BJMS) 7, S (August 26, 2020): S14—S17. http://dx.doi.org/10.48087/bjmstf.2020.s714.

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Le diagnostic de l’infection par le SARS-Cov-2 repose sur un ensemble d’arguments cliniques, biologiques et radiologiques. L’infection par le SARS-Cov-2 se manifeste principalement par des signes respiratoires. Parfois, des manifestations extra-respiratoires peuvent être le premier ou le seul symptôme de COVID-19, avant la fièvre ou les signes respiratoires. Les anomalies biologiques pouvant orienter précocement vers une infection par le SARS-CoV-2 sont la lymphopénie, une C Réactive Protéine (CRP) augmentée. Le scanner thoracique semble utile pour identifier des images compatibles avec la COVID-19. Les tests sérologiques pourraient amener une information diagnostique complémentaire aux tests moléculaires par RT-PCR, méthode de choix pour mettre en évidence la présence du virus SARS-CoV-2.

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Martignano, Filippo, Giorgia Gurioli, Samanta Salvi, Daniele Calistri, Matteo Costantini, Roberta Gunelli, Ugo De Giorgi, Flavia Foca, and Valentina Casadio. "GSTP1Methylation and Protein Expression in Prostate Cancer: Diagnostic Implications." Disease Markers 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/4358292.

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GSTP1belongs to the GSTs family, a group of enzymes involved in detoxification of exogenous substances and it also plays an important role in cell cycle regulation. Its dysregulation correlates with a large variety of tumors, in particular with prostate cancer. We investigatedGSTP1methylation status with methylation specific PCR (MS-PCR) in prostate cancer (PCa) and in benign tissue of 56 prostatectomies. We also performed immunohistochemistry (IHC) so as to correlate gene methylation with gene silencing.GSTP1appears methylated in PCa and not in healthy tissue; IHC confirmed that methylation leads to protein underexpression (p<0.001). GSTP1 is highly expressed in basal cell layer and luminal cells in benign glands while in prostatic intraepithelial neoplasia (PIN) it stains only basal cell layer, whereas PCa glands are completely negative. We demonstrated that methylation leads to underexpression of GSTP1. The progressive loss of GSTP1 expression from healthy glands to PIN and to PCa glands underlines its involvement in early carcinogenesis.

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Journal articles on the topic 'PCR diagnostic'

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