Log in

Relevant bibliographies by topics / Pcr isotherme / Journal articles

To see the other types of publications on this topic, follow the link: Pcr isotherme.

Journal articles on the topic 'Pcr isotherme'

Author: Grafiati

Published: 7 July 2024

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Pcr isotherme.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Zytner, Richard, Nihar Biswas, and Jatinder K. Bewtra. "Adsorption and desorption of perchloroethylene in soils, peat moss, and granular activated carbon." Canadian Journal of Civil Engineering 16, no. 6 (1989): 798–806. http://dx.doi.org/10.1139/l89-123.

Full text

Abstract:

Studies were conducted to evaluate the adsorption–desorption isotherms of a common dry cleaning solvent, perchloroethylene (PCE), in soils, peat moss, and granular activated carbon. The results obtained followed the Freundlich Isotherm, and the organic carbon content of the media was the most significant controlling factor in the adsorption–desorption process. The peat moss exhibited the highest residual saturation concentration for pure PCE amongst all the media tested. The desorption studies indicated that PCE had medium mobility in soil and was not significantly affected by moderate changes

APA, Harvard, Vancouver, ISO, and other styles

2

Shahrajabian, Mohamad H., Wenli Sun, and Qi Cheng. "Different Methods for Molecular and Rapid Detection of Human Novel Coronavirus." Current Pharmaceutical Design 27, no. 25 (2021): 2893–903. http://dx.doi.org/10.2174/1381612827666210604114411.

Full text

Abstract:

Introduction: While PCR has been recognized as one of the appropriate ways to diagnose infectious diseases, Loop-mediated isothermal amplification (LAMP) which is a nucleic acid amplification method, can be considered as an alternative to PCR, and it is faster, cost-effective, and easier to perform than nested PCR. Patients and Methods: Keywords were searched in PubMed/MEDLINE, Scopus and Institute for Scientific Information Web of Science, as well as the search engine of Google Scholar. Keywords included PCR, LAMP, RAA, RPA, Virus and COVID-19. Results: LAMP technology has been extensively ap

APA, Harvard, Vancouver, ISO, and other styles

3

Javed, Ifrah, Muhammad Asif Hanif, Umer Rashid, Farwa Nadeem, Fahad A. Alharthi, and Elham Ahmed Kazerooni. "Enhancing Functionalities in Nanocomposites for Effective Dye Removal from Wastewater: Isothermal, Kinetic and Thermodynamic Aspects." Water 14, no. 17 (2022): 2600. http://dx.doi.org/10.3390/w14172600.

Full text

Abstract:

The adsorption process combined with electrocoagulation is a highly effective technique for dye removal. Calcinized and non-calcinized composites based on bentonite and sodium zeolite were prepared for adsorptive removal of Foron EBL blue, Terasil brown 2RFL, Torque blue PG, and Orange P3R dyes. Factors affecting the adsorption process, such as contact time, initial dye concentration, and temperature, were also explored in this study. Equilibrium data of natural clay composites was explained by Freundlich, Langmuir, Dubinin Radushkevich isotherm, Harkin Jura, and Temkin isothermal models. Hark

APA, Harvard, Vancouver, ISO, and other styles

4

Megnassan, Sassou, and Koffi Jondo. "Oxydation à haute température de l’alliage Cu₅₄Ni₄₅Mn₁ : analyse isotherme." International Journal of Biological and Chemical Sciences 14, no. 3 (2020): 1144–52. http://dx.doi.org/10.4314/ijbcs.v14i3.39.

Full text

Abstract:

Dans la recherche de nouveaux matériaux possédant un meilleur comportement à l’oxydation haute température, les alliages cuivre-nickel sont de bons candidats grâce à leur résistance à l’oxydation. Cette étude est consacrée à la cinétique d’oxydation isotherme de l’alliage Cu54 Ni45 Mn1. Une étude de l’oxydation à haute température, de l’alliage Cu54Ni45Mn1 (constantan) dans l’oxygène a été réalisée. Les oxydations ont été réalisées par thermogravimétrie en mode isotherme, pour une gamme de températures comprises entre 650 °C et 900 °C et suivies à l'aide du microscope électronique à balayage (

APA, Harvard, Vancouver, ISO, and other styles

5

Onokebhagbe, Victor O., Abdulrahman Mukhtar, Salami D. Kazeem, and Jibo Abdullahi. "Effects of Selected Properties of Semi-Arid Soil of Dutse, Jigawa State on Adsorption of Pb and Cd Ions." UMYU Scientifica 2, no. 4 (2023): 92–101. http://dx.doi.org/10.56919/usci.2324.011.

Full text

Abstract:

This study determined the effects of some selected physical and chemical properties of semi-arid soil on heavy metal sorption. Soil samples were collected randomly from Federal University Dutse within Nigeria's Sudan Savannah agroecological zone. Nitrate salts were used to prepare three different concentrations (10, 20, 30 mg/kg) of CdCl2 and PbCl2. The equilibrium sorption of the study soil was determined. The equilibrium sorption data were fitted into Langmuir and Freundlich isotherms. Langmuir and Freundlich isotherm model's r2 values (1) were at par. The boundary energy b (1 mg-1) from the

APA, Harvard, Vancouver, ISO, and other styles

6

Yunita, Lisa, Dian Rachma Wijayanti, and Apriani Riyanti. "Sensitivitas Pemeriksaan Covid-19: Insulated Isothermal PCR (iiPCR) Dan Reverse Transcription PCR (RT-PCR)." JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST 6, no. 1 (2023): 37. http://dx.doi.org/10.30651/jmlt.v6i1.12441.

Full text

Abstract:

Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were colle

APA, Harvard, Vancouver, ISO, and other styles

7

OHKAWA, Tihiro. "Isothermal Confinement." Plasma and Fusion Research 2 (2007): 001. http://dx.doi.org/10.1585/pfr.2.001.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Rostamkhani, N., A. Haghnazari, M. Tohidfar, and A. Moradi. "Rapid identification of transgenic cotton (Gossypium hirsutum L.) plants by loop-mediated isothermal amplification." Czech Journal of Genetics and Plant Breeding 47, No. 4 (2011): 140–48. http://dx.doi.org/10.17221/7/2011-cjgpb.

Full text

Abstract:

In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was extracted from fresh leaf tissues of T<sub>2</sub> transgenic cotton containing chitinase (chi) and cry1A(b) genes. Detection of genes of interest was performed by polymerase chain reaction (PCR), LAMP and real-time PCR methods. In LAMP assay the amplification was performed after 30 min at 65&deg;C when loop primers were involved in the reaction. The involvement of loop primers decrea

APA, Harvard, Vancouver, ISO, and other styles

9

Park, Jee-Woong. "Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests." Biosensors 12, no. 10 (2022): 857. http://dx.doi.org/10.3390/bios12100857.

Full text

Abstract:

For the identification of nucleic acids, which are important biomarkers of pathogen-mediated diseases and viruses, the gold standard for NA-based diagnostic applications is polymerase chain reaction (PCR). However, the requirements of PCR limit its application as a rapid point-of-care diagnostic technique. To address the challenges associated with regular PCR, many isothermal amplification methods have been developed to accurately detect NAs. Isothermal amplification methods enable NA amplification without changes in temperature with simple devices, as well as faster amplification times compar

APA, Harvard, Vancouver, ISO, and other styles

10

Zanoli, Laura Maria, and Giuseppe Spoto. "Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices." Biosensors 3 (December 27, 2012): 18–43. https://doi.org/10.3390/bios3010018.

Full text

Abstract:

Diagnostic tools for biomolecular detection need to fulfill specific requirements in terms of sensitivity, selectivity and high-throughput in order to widen their applicability and to minimize the cost of the assay. The nucleic acid amplification is a key step in DNA detection assays. It contributes to improving the assay sensitivity by enabling the detection of a limited number of target molecules. The use of microfluidic devices to miniaturize amplification protocols reduces the required sample volume and the analysis times and offers new possibilities for the process automation and integrat

APA, Harvard, Vancouver, ISO, and other styles

11

Ahrberg, Christian D., Andreas Manz, and Bong Geun Chung. "Polymerase chain reaction in microfluidic devices." Lab on a Chip 16, no. 20 (2016): 3866–84. http://dx.doi.org/10.1039/c6lc00984k.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Bouam, Amar, Jean-Jacques Vincent, Elisabeth Le Glass, et al. "Rapid Isothermal Amplification for the Buccal Detection SARS-CoV-2 in the Context of Out-Patient COVID-19 Screening." Journal of Clinical Medicine 10, no. 12 (2021): 2643. http://dx.doi.org/10.3390/jcm10122643.

Full text

Abstract:

A commercially available isothermal amplification of SARS-CoV-2 RNA was applied to self-collected saliva samples using dry dental cotton rolls, which were held in the mouth for two minutes. Of 212 tests, isothermal amplification yielded three (0.14%) invalid results, 120 (56.6%) positive results and 89 (42%) negative results. Compared to reference RT-PCR assays routinely performed simultaneously on nasopharyngeal swabs, excluding the three invalid isothermal amplification assays and one RT-PCR invalid assay, these figures indicated that 119/123 (96.7%) samples were positive in both methods and

APA, Harvard, Vancouver, ISO, and other styles

13

Byun, Kye-Hwan, Sang Ha Han, Seungho Choi, et al. "Comparative efficacy of loop-mediated isothermal amplification, real-time PCR, and selective agar method for detection of Listeria monocytogenes in food." Korean Journal of Food Preservation 29, no. 3 (2022): 521–29. http://dx.doi.org/10.11002/kjfp.2022.29.3.521.

Full text

Abstract:

Listeria monocytogenes is a foodborne pathogen causing listeriosis, which can be fatal in specific high-risk groups. The aim of this study was to compare the performance (accuracy, sensitivity, and specificity) of 3M™ Molecular Detection System (3M™ MDS) and Korean Food Codex [real-time PCR (RT-PCR) and selective agar] for the detection of L. monocytogenes in various food matrices. The detection performance of the three methods was determined against 100-103 CFU/mL of L. monocytogenes in vitro and showed high accuracy in the order of RT-PCR, 3M™ MDS, and selective agar. There was no difference

APA, Harvard, Vancouver, ISO, and other styles

14

Sipho, Mfolozi. "Effects of Ground Temperature, Ground Material Properties and Body Position on Postmortem Axial Heat Transfer." Journal of Numerical Thanatochronometry 2, no. 1 (2022): 33–41. https://doi.org/10.5281/zenodo.6477742.

Full text

Abstract:

The effects of body position, ground temperature and ground material properties on postmortem axial heat transfer were investigated to inform numerical death-time estimation procedures. A postmortem cooling simulation in unforced convection conditions with no ground contact served as the control. Postmortem cooling was simulated in the supine position on a modelled cold concrete floor and on a domestic-type heated floor. Permanent axial displacement of the antemortem central isotherm (ACI) and postmortem central isotherms (PCI) away from the cold concrete ground and towards the heated floor wa

APA, Harvard, Vancouver, ISO, and other styles

15

Daher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples." Clinical Chemistry 60, no. 4 (2014): 660–66. http://dx.doi.org/10.1373/clinchem.2013.213504.

Full text

Abstract:

Abstract BACKGROUND Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity. METHODS We studied the clinical performance of recombinase polymerase amplification for the screening of group B streptococci in vaginal/anal samples from 50 pregnant women. We also compared

APA, Harvard, Vancouver, ISO, and other styles

16

Du, Tian, Ji-hong Lin, Jun-hua Zhao, Hai-bo Wang, and Qiu-hua Mo. "Development and Evaluation of an iiPCR Assay for Salmonella and Shigella Detection on a Field-Deployable PCR System." Canadian Journal of Infectious Diseases and Medical Microbiology 2020 (September 7, 2020): 1–5. http://dx.doi.org/10.1155/2020/9373984.

Full text

Abstract:

Background. Salmonella and Shigella are often associated with fecal-oral transmission and cause large-scale outbreaks in centralized catering units and, therefore, should be frequently and strictly monitored, especially among food handlers. However, no specific and sensitive on-site detection method is available until now. Methods. In this study, an insulated isothermal PCR assay for the detection of Salmonella and Shigella on a field-deployable PCR system was developed. Specificity, sensitivity, reproducibility, and clinical accuracy of the assay were characterized and evaluated. Results. The

APA, Harvard, Vancouver, ISO, and other styles

17

Maltzeva, Yulia I., Daria A. Gorbenko, Ekaterina V. Nikitina, Maria S. Rubel, and Dmitry M. Kolpashchikov. "Visual Detection of Stem-Loop Primer Amplification (SPA) Products without Denaturation Using Peroxidase-like DNA Machines (PxDM)." International Journal of Molecular Sciences 24, no. 9 (2023): 7812. http://dx.doi.org/10.3390/ijms24097812.

Full text

Abstract:

Rapid, inexpensive, and accurate determination of nucleic acids is a decisive factor in evaluating population’s health and monitoring treatment at point-of-care (POC) settings. Testing systems with visual outputs can provide instrument-free signal detection. Isothermal amplification technologies can substitute conventional polymerase chain reaction (PCR) testing due to compatibility with the POC diagnostic. Here, we have visually detected DNA fragments obtained by stem-loop-primer-assisted isothermal amplification (SPA), but not those obtained by PCR or LAMP amplification using DNA nanomachine

APA, Harvard, Vancouver, ISO, and other styles

18

Chen, Jyh Jian, Wei Hua Chen, and Yi Shiang Shie. "The Effect of Thermal Contact Resistance on Heat Management in a Shuttling PCR System." Applied Mechanics and Materials 284-287 (January 2013): 1941–45. http://dx.doi.org/10.4028/www.scientific.net/amm.284-287.1941.

Full text

Abstract:

A novel shuttling polymerase chain reaction (PCR) system is assembled to make temperature uniform in the reaction chamber. The chamber is oscillated by a servo motor and contacted with three different isothermal zones to complete several thermal cycles. The home-made computer code is utilized to investigate the influences of operational parameters on the temperature inside the chamber. Numerical results show that the contact resistances between the heating blocks and the reaction chamber dominate the temperatures inside the PCR chamber. In this work a PCR system that is composed of the PID con

APA, Harvard, Vancouver, ISO, and other styles

19

TSEN, HAU-YANG, CHIA-MING SHIH, PING-HUA TENG, et al. "Detection of Salmonella in Chicken Meat by Insulated Isothermal PCR." Journal of Food Protection 76, no. 8 (2013): 1322–29. http://dx.doi.org/10.4315/0362-028x.jfp-12-553.

Full text

Abstract:

Consumption of Salmonella-contaminated foods, such as poultry and fresh eggs, is known to be one of the main causes of salmonellosis. Conventional PCR methods, including real-time PCR for rapid detection of Salmonella, in general require skilled technicians and costly instruments. A recently developed novel convective PCR, insulated isothermal PCR (iiPCR), is carried out in polycarbonate capillary tubes. In this study, we designed TaqMan probes and PCR primers based on the yrfH gene encoding a heat shock protein for the iiPCR detection of Salmonella in chicken meat samples. The TaqMan probe wa

APA, Harvard, Vancouver, ISO, and other styles

20

Tan, Kim-Kee, Noor Syahida Azizan, Che Norainon Yaacob, et al. "Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus." BMC Infectious Diseases 18, no. 1 (2018): 169. https://doi.org/10.1186/s12879-018-3065-1.

Full text

Abstract:

<strong>Background: </strong>A method for rapid detection of dengue virus using the reverse-transcription recombinase polymerase amplification (RT-RPA) was recently developed, evaluated and made ready for deployment. However, reliance solely on the evaluation performed by experienced researchers in a well-structured and well-equipped reference laboratory may overlook the potential intrinsic problems that may arise during deployment of the assay into new application sites, especially for users unfamiliar with the test. Appropriate assessment of this newly developed assay by users who are unfami

APA, Harvard, Vancouver, ISO, and other styles

21

Tan, Vivienne J., Ying Liang, Arnold S. Tan, et al. "A Strategy Potentially Suitable for Combined Preimplantation Genetic Testing of Aneuploidy and Monogenic Disease That Permits Direct Detection of Pathogenic Variants Including Repeat Expansions and Gene Deletions." International Journal of Molecular Sciences 26, no. 10 (2025): 4532. https://doi.org/10.3390/ijms26104532.

Full text

Abstract:

Combined preimplantation genetic testing of aneuploidy (PGT-A) and monogenic disease (PGT-M) can be achieved through PCR-based whole genome amplification (WGA) and next-generation sequencing (NGS). However, pathogenic variant detection is usually achieved indirectly through single nucleotide polymorphism haplotyping, as direct detection of pathogenic variants is not always possible. We evaluated whether isothermal WGA was suitable for combined PGT-A and PGT-M that also permitted direct detection of repeat expansions and large deletions, in addition to indirect linkage analysis using microsatel

APA, Harvard, Vancouver, ISO, and other styles

22

Létoffé, J. M., P. Claudy, J. P. Planche, and L. Germanaud. "Caractérisation de l'interaction bitume-agrégat par microcalorimétrie isotherme." Thermochimica Acta 210 (November 1992): 27–40. http://dx.doi.org/10.1016/0040-6031(92)80274-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Reif, John H., and Urmi Majumder. "Isothermal reactivating Whiplash PCR for locally programmable molecular computation." Natural Computing 9, no. 1 (2009): 183–206. http://dx.doi.org/10.1007/s11047-009-9148-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

James, Ameh, and John Alawneh. "COVID-19 Infection Diagnosis: Potential Impact of Isothermal Amplification Technology to Reduce Community Transmission of SARS-CoV-2." Diagnostics 10, no. 6 (2020): 399. http://dx.doi.org/10.3390/diagnostics10060399.

Full text

Abstract:

The current coronavirus disease 2019 (COVID-19) pandemic is largely driven by community transmission, after 2019 novel Coronavirus (2019-nCoV or SARS-CoV-2) crosses the borders. To stop the spread, rapid testing is required at community clinics and hospitals. These rapid tests should be comparable with the standard PCR technology. Isothermal amplification technology provides an excellent alternative that is highly amenable to resource limited settings, where expertise and infrastructure to support PCR are not available. In this review, we provide a brief description of isothermal amplification

APA, Harvard, Vancouver, ISO, and other styles

25

Maruyama, Fumito, Takehiko Kenzaka, Nobuyasu Yamaguchi, Katsuji Tani, and Masao Nasu. "Detection of Bacteria Carrying the stx2 Gene by In Situ Loop-Mediated Isothermal Amplification." Applied and Environmental Microbiology 69, no. 8 (2003): 5023–28. http://dx.doi.org/10.1128/aem.69.8.5023-5028.2003.

Full text

Abstract:

ABSTRACT A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was used to detect stxA 2 in Escherichia coli O157:H7 cells. The mild permeabilization conditions and low isothermal temperature used in the in situ LAMP method caused less cell damage than in situ PCR. It allowed use of fluorescent antibody labeling in the bacterial mixture after the DNA amplification for identification of E. coli O157:H7 cells with an stxA 2 gene. Higher-contrast images were obtained with this method tha

APA, Harvard, Vancouver, ISO, and other styles

26

Eckel, Florian, Franziska Küsters, Bernhard Drossel, Markus Konert, Hans Mattes, and Stefan Schopf. "Variplex™ test system fails to reliably detect SARS-CoV-2 directly from respiratory samples without RNA extraction." European Journal of Clinical Microbiology & Infectious Diseases 39, no. 12 (2020): 2373–77. http://dx.doi.org/10.1007/s10096-020-03983-9.

Full text

Abstract:

AbstractDiagnosis of COVID is performed by PCR methods, but their capacity is limited by the requirement of high-level facilities and instruments. The loop-mediated isothermal amplification (LAMP) method has been utilized for the detection of isolated virus-specific RNA. Preliminary data suggest the possibility of isothermal amplification directly from respiratory samples without RNA extraction. All patients admitted to our hospital were screened for SARS-CoV-2 by routine. Respiratory samples were tested by variplex system based on LAMP method directly without RNA extraction and by PCR. Primar

APA, Harvard, Vancouver, ISO, and other styles

27

Safwat, Safwat M., Nouran Y. Mohamed, Mohamed N. A. Meshref, and Abdelsalam Elawwad. "Adsorption of Phenol onto Aluminum Oxide Nanoparticles: Performance Evaluation, Mechanism Exploration, and Principal Component Analysis (PCA) of Thermodynamics." Adsorption Science & Technology 2022 (April 4, 2022): 1–14. http://dx.doi.org/10.1155/2022/1924117.

Full text

Abstract:

The removal of phenolic compounds from aqueous solutions using novel adsorption techniques becomes a key research item. Of those, nanoparticles in particular, the low-cost and the high-strength aluminum oxide nanoparticles showed promising results in pollutant uptake and increase in the adsorption efficiency. This study examined various physicochemical process parameters such as temperature, pH, initial phenol concentration, and adsorbent doses, in addition to the impact of those parameters on the adsorption removal mechanism of phenol. The results highlighted that aluminum oxide nanoparticles

APA, Harvard, Vancouver, ISO, and other styles

28

Fomicheva, K. A., A. I. Osipyants, M. Y. Shkurnikov, A. A. Pokryschenko, E. A. Tonevitsky, and V. I. Vechorko. "Loop-mediated isothermal amplification holds great potential for massive COVID-19 diagnostics." Biotekhnologiya 36, no. 5 (2020): 6–12. http://dx.doi.org/10.21519/0234-2758-2020-36-5-6-12.

Full text

Abstract:

Real-time RT-PCR currently remains most popular for early COVID-19 diagnostics. However Loop-mediated isothermal amplification (LAMP) method outperform real-time RT-PCR in rapidity and simplicity because it doesn't require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing are already exist, but LAMP-based tests are not yet widely adopted. The method has great potential for mass application. Here we discuss technical and methodological aspects of its widespread implementation. COVID-19, SARS-CoV-2, LAMP, loop-mediated isothermal amplification,

APA, Harvard, Vancouver, ISO, and other styles

29

Edlyn, Mae N. Ciano. "Prevalence of Leptospira spp. in Urine of Rats (Rattus spp.) in an Urban Village in the Philippines using LAMP and PCR Assays." International Journal of Environmental and Agriculture Research 11, no. 4 (2025): 28–33. https://doi.org/10.5281/zenodo.15300708.

Full text

Abstract:

<strong>Abstract</strong><strong>—</strong> Rats are recognized as key reservoirs and potential transmission sources of leptospirosis. Despite this, limited data exist on the prevalence of Leptospira spp. among rats in urban villages of the Philippines. This study aimed to assess the presence of Leptospira spp. in rats from an urban village in Benguet Province. Urine samples from 50 rats were analyzed using Loop-Mediated Isothermal Amplification (LAMP) and Polymerase Chain Reaction (PCR) assays. Results showed a Leptospira spp. detection rate of 30% using LAMP and 18% using PCR. DNA sequ

APA, Harvard, Vancouver, ISO, and other styles

30

Soroka, Marianna, Barbara Wasowicz, and Anna Rymaszewska. "Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?" Cells 10, no. 8 (2021): 1931. http://dx.doi.org/10.3390/cells10081931.

Full text

Abstract:

In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. O

APA, Harvard, Vancouver, ISO, and other styles

31

Shi, Chao, Fanjin Shang, Meiling Zhou, Pansong Zhang, Yifan Wang, and Cuiping Ma. "Triggered isothermal PCR by denaturation bubble-mediated strand exchange amplification." Chemical Communications 52, no. 77 (2016): 11551–54. http://dx.doi.org/10.1039/c6cc05906f.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Lim, King Ting, Cindy Shuan Ju Teh, and Kwai Lin Thong. "Loop-Mediated Isothermal Amplification Assay for the Rapid Detection ofStaphylococcus aureus." BioMed Research International 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/895816.

Full text

Abstract:

Staphylococcus aureus, including methicillin-resistantS. aureus(MRSA), is an important human pathogen that produces a variety of toxins and causes a wide range of infections, including soft-tissue infections, bacteremia, and staphylococcal food poisoning. A loop-mediated isothermal amplification (LAMP) assay targeting thearcCgene ofS. aureuswas developed and evaluated with 119S. aureusand 25 non-S. aureusstrains. The usefulness of the assay was compared with the PCR method that targetsspaandarcCgenes. The optimal temperature for the LAMP assay was 58.5°C with a detection limit of 2.5 ng/μL and

APA, Harvard, Vancouver, ISO, and other styles

33

Johan, Calvin Wijaya, Sulistyo Emantoko Dwi Putra, and Ernest Suryadjaja. "Deteksi Vibrio harveyi dengan Metode Amplifikasi DNA pada Gen toxR." KELUWIH: Jurnal Sains dan Teknologi 1, no. 1 (2020): 29–37. http://dx.doi.org/10.24123/saintek.v1i1.2775.

Full text

Abstract:

Abstrak -Infeksi hewan akuakultur oleh Vibrio harveyi dapat menyebabkan kematian serta kerugian ekonomi. Kemampuan untuk mendeteksi bakteri tersebut secara dini dapat mencegah penyebarannya dalam akuakultur. Penelitian ini bertujuan untuk mengembangkan metode untuk mendeteksi Vibrio harveyi melalui amplifikasi gen toxR. Amplifikasi DNA dilakukan dengan dua metode, yakni amplifikasi isotermal termediasi loop (loop-mediated isothermal amplification, LAMP) dan reaksi berantai polimerase (PCR). Amplifikasi menggunakan metode LAMP menunjukan perlu dilakukan optimasi protokol maupun desain primer un

APA, Harvard, Vancouver, ISO, and other styles

34

Gicheha, Daisaku, Aicha Noura Cisse, Ariful Bhuiyan та Nabila Shamim. "Non-Isothermal Crystallization Kinetics of Poly (ɛ-Caprolactone) (PCL) and MgO Incorporated PCL Nanofibers". Polymers 15, № 14 (2023): 3013. http://dx.doi.org/10.3390/polym15143013.

Full text

Abstract:

The study delves into the kinetics of non-isothermal crystallization of Poly (ɛ-caprolactone) (PCL) and MgO-incorporated PCL nanofibers with varying cooling rates. Differential Scanning Calorimetry (DSC-3) was used to acquire crystallization information and investigate the kinetics behavior of the two types of nanofibers under different cooling rates ranging from 0.5–5 K/min. The results show that the crystallization rate decreases at higher crystallization temperatures. Furthermore, the parameters of non-isothermal crystallization kinetics were investigated via several mathematical models, in

APA, Harvard, Vancouver, ISO, and other styles

35

García-Bernalt Diego, Juan, Pedro Fernández-Soto, Begoña Febrer-Sendra, Beatriz Crego-Vicente, and Antonio Muro. "Loop-Mediated Isothermal Amplification in Schistosomiasis." Journal of Clinical Medicine 10, no. 3 (2021): 511. http://dx.doi.org/10.3390/jcm10030511.

Full text

Abstract:

Human schistosomiasis is one of the most important parasitic diseases, causing around 250 million cases (mostly in Africa) and 280,000–500,000 deaths every year. Due to the limited resources and the far-removed nature of many endemic areas, the implementation of new, sensitive and specific diagnostic tools has had little success. This is particularly true for PCR-based molecular methods that require expensive equipment and trained personnel to be executed. Loop-mediated isothermal amplification (LAMP) along with other isothermal techniques appeared in the early 21st century as an alternative t

APA, Harvard, Vancouver, ISO, and other styles

36

YOKOYAMA, EIJI, MASAKO UCHIMURA, and KENITIRO ITO. "Detection of Enteroaggregative Escherichia coli by Loop-Mediated Isothermal Amplification." Journal of Food Protection 73, no. 6 (2010): 1064–72. http://dx.doi.org/10.4315/0362-028x-73.6.1064.

Full text

Abstract:

A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia coli (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than t

APA, Harvard, Vancouver, ISO, and other styles

37

Sharafdarkolaee, Somayeh Heidari, Pooria Gill, Majid Motovali-Bashi, and Fatemeh Heidari Sharafdarkolaee. "Isothermal Amplification Methods for the SNP Genotyping." Current Molecular Medicine 19, no. 7 (2019): 461–72. http://dx.doi.org/10.2174/1566524019666190527083947.

Full text

Abstract:

The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced. The best alternatives were the isothermal amplification technologies that those did not require a thermal cycler. In this review, we aimed to describe the most known isothermal amplification techniques for SNP genotyping.

APA, Harvard, Vancouver, ISO, and other styles

38

Benmansour, Abdeldjellil, and Mohammad Hamdan. "Simulation du Stockage de l'Energie Thermique dans un Lit Fixe de Sphères Contenant un Matériau à Changement de Phase." Journal of Renewable Energies 4, no. 2 (2001): 125–34. http://dx.doi.org/10.54966/jreen.v4i2.1004.

Full text

Abstract:

Ce travail présente une étude numérique du stockage de l'énergie thermique par chaleur latente à basses températures (0 °C à 100 °C) dans un lit cylindrique rempli de sphères uniformes, disposées au hasard et contenant chacune un matériau à changement de phase (MCP), traversé par un flux d’air. Un modèle numérique mono dimensionnel à deux phases séparées est appliqué. Il a permis de prédire la distribution axiale de la température du fluide et du matériau fusible le long du lit ainsi que les performances de ce lit fixe dans les deux modes de stockage et de récupération de la chaleur pour une t

APA, Harvard, Vancouver, ISO, and other styles

39

Lung, O., J. Pasick, M. Fisher, C. Buchanan, A. Erickson, and A. Ambagala. "Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus." Transboundary and Emerging Diseases 63, no. 5 (2015): e395-e402. http://dx.doi.org/10.1111/tbed.12318.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Das, Debayan, Manaswini Masetty, and Aashish Priye. "Paper-Based Loop Mediated Isothermal Amplification (LAMP) Platforms: Integrating the Versatility of Paper Microfluidics with Accuracy of Nucleic Acid Amplification Tests." Chemosensors 11, no. 3 (2023): 163. http://dx.doi.org/10.3390/chemosensors11030163.

Full text

Abstract:

Paper-based diagnostics offer a promising alternative to traditional diagnostic methods for point-of-care use due to their low cost, ease of use, portability, rapid results, versatility, and low environmental impact. While paper-based serology tests in the form of lateral flow assays can provide rapid test results for past pathogen exposure, they currently lack the accuracy and sensitivity offered by molecular diagnostic tests such as the polymerase chain reaction (PCR). Loop-mediated isothermal amplification (LAMP)—an isothermal nucleic acid amplification test (NAAT)—provides PCR-like perform

APA, Harvard, Vancouver, ISO, and other styles

41

Nikiforov, K. A. "Advanced Molecular-Genetic Methods and Prospects for Their Application for the Indication and Identification of <i>Yersinia pestis</i> Strains." Problems of Particularly Dangerous Infections, no. 4 (February 11, 2023): 29–40. http://dx.doi.org/10.21055/0370-1069-2022-4-29-40.

Full text

Abstract:

The review provides an analysis of the literature data on the use of various modern molecular-genetic methods for the indication and identification of Yersinia pestis strains with different properties and degree of virulence, which is caused by the diverse natural conditions in which they circulate. The methods are also considered from the perspective of their promising application at three levels (territorial, regional and federal) of the system for laboratory diagnosis of infectious diseases at the premises of Rospotrebnadzor organizations to solve the problem of maintaining the sanitary and

APA, Harvard, Vancouver, ISO, and other styles

42

Yusop, M. H. M., and M. F. A. Bakar. "Review on halal forensic: a focus on DNA-based methods for pork authentication." Food Research 4, no. 6 (2020): 2347–54. http://dx.doi.org/10.26656/fr.2017.4(6).180.

Full text

Abstract:

Food product authentication is important at every level of the food manufacturing process, starting from raw materials until finished products. Authentication also plays an important role in assuring accurate food labelling, which is required to help consumers select suitable types of food products. Food adulteration is one of the vital issues addressed by halal authentication, especially for food products that contain pig traces or porcine ingredients. Various methods that aim to guarantee the authenticity of foods have been developed over the past years. In this article, a short review of re

APA, Harvard, Vancouver, ISO, and other styles

43

Shiluli, Clement, Shwetha Kamath, Bernard N. Kanoi, et al. "Multi-repeat sequences identification using genome mining techniques for developing highly sensitive molecular diagnostic assay for the detection of Chlamydia trachomatis." Open Research Africa 7 (May 9, 2024): 2. http://dx.doi.org/10.12688/openresafrica.14316.2.

Full text

Abstract:

Chlamydia trachomatis (C. trachomatis) is a common sexually transmitted infection (STI). In 2019, the World Health Organization reported about 131 million infections. The majority of infected patients are asymptomatic with cases remaining undetected. It is likely that missed C. trachomatis infections contribute to preventable adverse health outcomes in women and children. Consequently, there is an urgent need of developing efficient diagnostic methods. In this study, genome-mining approaches to identify identical multi-repeat sequences (IMRS) distributed throughout the C. trachomatis genome we

APA, Harvard, Vancouver, ISO, and other styles

44

Shiluli, Clement, Shwetha Kamath, Bernard N. Kanoi, et al. "Multi-repeat sequences identification using genome mining techniques for developing highly sensitive molecular diagnostic assay for the detection of Chlamydia trachomatis." Open Research Africa 7 (January 8, 2024): 2. http://dx.doi.org/10.12688/openresafrica.14316.1.

Full text

Abstract:

Chlamydia trachomatis (C. trachomatis) is a common sexually transmitted infection (STI). In 2019, the World Health Organization reported about 131 million infections. The majority of infected patients are asymptomatic with cases remaining undetected. It is likely that missed C. trachomatis infections contribute to preventable adverse health outcomes in women and children. Consequently, there is an urgent need of developing efficient diagnostic methods. In this study, genome-mining approaches to identify identical multi-repeat sequences (IMRS) distributed throughout the C. trachomatis genome we

APA, Harvard, Vancouver, ISO, and other styles

45

Buffart, Tineke E., Marianne Tijssen, Thijs Krugers, et al. "DNA Quality Assessment for Array CGH by Isothermal Whole Genome Amplification." Analytical Cellular Pathology 29, no. 4 (2007): 351–59. http://dx.doi.org/10.1155/2007/709290.

Full text

Abstract:

Background: Array Comparative Genomic Hybridization (array CGH) is increasingly applied on DNA obtained from formalin-fixed paraffin-embedded (FFPE) tissue, but in a proportion of cases this type of DNA is unsuitable. Due to the high experimental costs of array CGH and unreliable methods for DNA quality testing, better prediction methods are needed. The aim of this study was to accurately determine the quality of FFPE DNA input in order to predict quality of array CGH outcome. Material and Methods: DNA quality was assessed by isothermal amplification and compared to array CGH quality on 59 FFP

APA, Harvard, Vancouver, ISO, and other styles

46

Areekit, Supatra, Pongbun Tangjitrungrot, Sintawee Khuchareontaworn, et al. "Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2)." Current Issues in Molecular Biology 44, no. 11 (2022): 5427–39. http://dx.doi.org/10.3390/cimb44110368.

Full text

Abstract:

Porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 2 (PCV2) are both important global pathogenic viruses which have a significant impact on the swine industry. In this study, a duplex loop-mediated isothermal amplification (duplex LAMP) method was developed in combination with lateral flow dipstick (LFD) for simultaneous detection of PEDV and PCV2 using specific sets of primers and probes designed based on the conserved regions of a spike gene (KF272920) and an ORF gene (EF493839), respectively. The limit of detection (LOD) values of the duplex LAMP-LFD for the detection of PED

APA, Harvard, Vancouver, ISO, and other styles

47

PARTHIBAN, M., K. S. SHALINI, KURUNCHI C. DIVYA, K. KUMANAN, and K. S. AARTHI. "Rapid detection of fowl adenovirus field samples using loop-mediated isothermal amplification assay." Indian Journal of Animal Sciences 84, no. 1 (2014): 22–25. http://dx.doi.org/10.56093/ijans.v84i1.37295.

Full text

Abstract:

Loop-mediated isothermal amplification was employed for the detection of fowl adenoviruses as a simple, rapid and field based diagnostic assay. Out of 134 field samples screened, 28 samples were positive by both PCR and LAMP assay. The LAMP assay primers were found to be highly specific and it detected only fowl adenovirus samples and it did not react with other avian viruses like Marek’s disease virus and avian leucosis virus. Conventional PCR detected 100ug level of DNA whereas LAMP assay detected up to 10ng level of DNA. It clearly indicated that LAMP assay was 100-times more sensitive than

APA, Harvard, Vancouver, ISO, and other styles

48

Zhong, Qing Ping, Li Wang, Bin Wang, and Hong Yuan Chen. "Loop-Mediated Isothermal Amplification Method for Rapid Detection of Shigella dysenteriae." Applied Mechanics and Materials 140 (November 2011): 369–73. http://dx.doi.org/10.4028/www.scientific.net/amm.140.369.

Full text

Abstract:

The study was aimed to develop a loop-mediated isothermal amplification (LAMP) method which amplifies DNA with high specificity and rapidity for the detection of Shigella dysenteriae. A set of four primers was designed for recognizing six distinct sequences on the target ipaH of S. dysenteriae. By the method, the target DNA was amplified within 1h under isothermal condition at 65 °C. The sensitivities of the LAMP for detecting pure culture and genomic DNA were 1.04 CFU/ml and 1.06 fg/μl, while the sensitivities of PCR method were 1.04×102 CFU/ml and 1.06 pg/μl. Furthermore, the LAMP assay was

APA, Harvard, Vancouver, ISO, and other styles

49

Murwantoko, Murwantoko. "Metode Loop-Mediated Isothermal Amplification (LAMP) dan Aplikasinya untuk Deteksi Penyakit Ikan." Jurnal Perikanan Universitas Gadjah Mada 8, no. 1 (2006): 1. http://dx.doi.org/10.22146/jfs.156.

Full text

Abstract:

Polymerase chain reaction (PCR) is a method that amplifies DNA which have been widely used in molecular biology technique. Based on the PCR, many methods have been developed on isothermal condition and the useful one is loop-mediated isothermal amplification of DNA (LAMP). LAMP reaction employs a Bst DNA polymerase and a set of four specific primers that recognizes a total of six distinct sequences of the target DNA and produces amount of different size of DNA. Many advantages have been achieved in LAMP such as the simple equipment for reaction and observation, short time, highly specific and

APA, Harvard, Vancouver, ISO, and other styles

50

Hussain, Musaddeq. "Isothermal droplet digital PCR method for quantification of CHO residual DNA." Journal of Pharmaceutical and Biomedical Analysis 211 (March 2022): 114564. http://dx.doi.org/10.1016/j.jpba.2021.114564.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!