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Journal articles on the topic 'PCR-RFLP markers'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Dolanská, L., and V. Čurn. "Identification of white clover (Trifolium repens L.) cultivars using molecular markers." Plant, Soil and Environment 50, No. 3 (2011): 95–100. http://dx.doi.org/10.17221/4013-pse.

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The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability o

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2

Savichtcheva, O., and S. Okabe. "Qualitative and quantitative estimation of host-specific fecal pollution using Bacteroides–Prevotella 16S rRNA genetic markers by T-RFLP and real-time PCR analyses." Water Science and Technology 59, no. 9 (2009): 1831–40. http://dx.doi.org/10.2166/wst.2009.213.

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Rapid and reliable determination of the non-point sources of fecal pollution is a critical issue for the environmental microbiologists all over the world. In this work we evaluated the use of anaerobic bacterial group Bacteroides–Prevotella as an alternative fecal pollution indicator. Terminal restriction fragment length polymorphism (T-RFLP) and real-time polymerase chain reaction (RT-PCR) analyses were used to monitor and quantify human-, cow- and pig-specific fecal contamination in natural river waters. We also included DNA sequence analysis of the identified fecal markers revealed by T-RFL

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3

Mayes, S., P. L. Jack, R. H. V. Corley, and D. F. Marshall. "Construction of a RFLP genetic linkage map for oil palm (Elaeis guineensis Jacq.)." Genome 40, no. 1 (1997): 116–22. http://dx.doi.org/10.1139/g97-016.

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We have developed a restriction fragment length polymorphism (RFLP) genetic map in oil palm (Elaeis guineensis Jacq.) for use in breeding programmes. A segregating population of 98 individuals was probed with 84 informative low copy clones (mainly PstI genomics). This yielded 103 scorable loci, of which 97 could be linked into 24 groups of two or more markers (n = 16 for oil palm), encompassing a total of 860 cM. The high level of linkage between markers (95%) suggests good genome coverage and very little segregation distortion of markers was observed. The mapping population, which was generat

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4

GHIMIRE, S. C., and J. R. EGERTON. "PCR–RFLP of outer membrane proteins gene of Dichelobacter nodosus: a new tool in the epidemiology of footrot." Epidemiology and Infection 122, no. 3 (1999): 521–28. http://dx.doi.org/10.1017/s0950268899002290.

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Currently only phenotypic epidemiological markers, serogrouping and virulence testing of Dichelobacter nodosus, are available for investigating footrot outbreaks in small ruminants. These methods have limitations in tracing the source of infection. In this study, a genotypic marker, PCR–RFLP of outer membrane protein gene, was used to characterize D. nodosus. The technique was evaluated in a controlled experiment involving two strains of bacteria. PCR–RFLP was found to be highly specific in differentiating isolates obtained from recipient animals infected with different strains. Subsequently,

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5

Kauserud, Håvard, and Trond Schumacher. "Population structure of the endangered wood decay fungus Phellinus nigrolimitatus (Basidiomycota)." Canadian Journal of Botany 80, no. 6 (2002): 597–606. http://dx.doi.org/10.1139/b02-040.

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The population structure of five Fennoscandian geographic populations of the endangered wood-decay fungus Phellinus nigrolimitatus (Romell) Bourdot et Galzin was examined by analyses of nuclear ribosomal DNA (nrDNA) spacer sequences (ITS and IGS1) and a partial sequence of the elongation factor 1α gene (efa). A high level of sequence variation was observed in ITS and IGS1, suggesting restrictions in nrDNA homogenization in this taxon. Six polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) markers, five located in nrDNA and one in efa, suggest that the geographic po

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Mudaningrat, Ajeng, Flora Umaya, Farah Ayu Afdhila Syahriza, Yustinus Ulung Anggraito, and Ning Setiati. "LITERATURE REVIEW: APLIKASI PENANDA MOLEKULER UNTUK ANALISIS KEANEKARAGAMAN GENETIK HEWAN." BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan 10, no. 1 (2023): 11–25. http://dx.doi.org/10.30598/biopendixvol10issue1page11-25.

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Background: Molecular markers (DNA markers) are DNA sequences that are easily detected to characterize or detect certain genetic diversity, one of which is in animals. There are various marker systems used with their respective characteristics. Some of the molecular markers include RFLP, RAPD, ISSR, SSR, and SNP. The purpose of this study was to analyze genetic diversity in animals using molecular marker applications.Methods: The method used is literature review using reputable national and international journals.Results: Based on the results of a literature review, the genetic diversity of va

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VICTOIR, K., A. L. BAÑULS, J. AREVALO, et al. "The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia." Parasitology 117, no. 1 (1998): 1–13. http://dx.doi.org/10.1017/s0031182098002789.

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In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a refe

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8

Nuroniah, H. S., O. Gailing, and R. Finkeldey. "Development of SCAR Markers for Species Identification in the Genus Shorea (Dipterocarpaceae)." Silvae Genetica 59, no. 1-6 (2010): 249–57. http://dx.doi.org/10.1515/sg-2010-0035.

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AbstractThe development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification of tropical timber is of particular interest in this context. We describe the development of SCAR (Sequence Characterized Amplified Region) markers for forensic applications taking the example of two closely related species of the tropical tree family Shorea (Dipterocarpaceae). Two AFLP (Amplified Fragment Length Polymorphism) fragments have been described earlier showing strong differentiation between S. leprosula and S. parvifol

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Shibuya, H., B. K. Collins, S. J. Stoy, D. Nonneman, and G. S. Johnson. "PCR/RFLP markers in the canine gamma-crystallin gene." Animal Genetics 26, no. 6 (2009): 445–46. http://dx.doi.org/10.1111/j.1365-2052.1995.tb02700.x.

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10

Khatoon, Arifa, Sumeet Verma, Gayatri Wadiye, and Anuprita Zore. "Molecular markers and their potentials." International Journal of Bioassays 5, no. 01 (2016): 4706. http://dx.doi.org/10.21746/ijbio.2016.01.003.

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The use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant molecular biotechnology and their genetic studies. There are three different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiating in two types 1. Non PCR based (RFLP) and 2. PCR based markers (RAPD, AFLP, SSR, SNP etc.). Amongst others, the microsatellite DNA marker is one of the most widely used marker due to its easy use by simple PCR, followed by a denaturing gel electrophoresis. SNP (Single Nucleotide Po

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11

Abdel-Rahman, S. M. "Evidences reveal that cattle and buffalo evolutionary derived from the same ancestor based on cytogenetic and molecular markers." Biotehnologija u stocarstvu 22, no. 3-4 (2006): 1–9. http://dx.doi.org/10.2298/bah0604001a.

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Muscle-DNA from cattle and buffalo was extracted to amplify the mitochondrial DNA segment (cytochrome b gene) and the gene encoding species-specific repeat (SSR) region. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and SSR techniques were used to identify of species origin. Restriction analysis of PCR-RFLP of the mitochondrial cytochrome b segment and SSR analysis showed no differences between cattle and buffalo. Where, the fragment length (bp) generated by AluI PCR-RFLP were 190, 169 and PCR amplification size of the gene encoding SSR region was 603 bp in both

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12

Sinjare, Dalal Y. Kh. "Molecular Identification of Morus ssp. in Duhok Using Nuclear ITS Region and Chloroplast Matk Gene." Basrah Journal of Agricultural Sciences 37, no. 1 (2024): 86–93. http://dx.doi.org/10.37077/25200860.2024.37.1.07.

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Since Mulberries (Morus)is a tree species with a considerable plant variety. Molecular techniques are methods used to distinguish between species accurately, easily and quickly. This study examines a Molecular method for distinguishing different Morus species in the Duhok - Kurdistan region/ Iraq. The method is based on the use of four techniques: matK gene, the ITS region, PCR-RFLP, and SRAP markers. Twelve Morus species have been selected for this study from different region of Duhok. The ITS region's PCR result was 700 bp, but the matK gene's PCR produce was 900 bp. The same restriction sit

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Carew, M. E., V. Pettigrove, and A. A. Hoffmann. "Identifying chironomids (Diptera: Chironomidae) for biological monitoring with PCR–RFLP." Bulletin of Entomological Research 93, no. 6 (2003): 483–90. http://dx.doi.org/10.1079/ber2003268.

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AbstractChironomids are excellent biological indicators for the health of aquatic ecosystems, but their use at finer taxonomic levels is hindered by morphological similarity of species at each life stage. Molecular markers have the potential to overcome these problems by facilitating species identification particularly in large-scale surveys. In this study, the potential of the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) approach was tested to rapidly distinguish among chironomids within a geographic area, by considering chironomid species from Melbourne, Aust

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14

Erpelding, J. E., N. K. Blake, T. K. Blake, and L. E. Talbert. "Transfer of sequence tagged site PCR markers between wheat and barley." Genome 39, no. 4 (1996): 802–10. http://dx.doi.org/10.1139/g96-101.

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Transfer of mapping information between related species has facilitated the development of restriction fragment length polymorphism (RFLP) maps in the cereals. Sequence tagged site (STS) primer sets for use in the polymerase chain reaction may be developed from mapped RFLP clones. For this study, we mapped 97 STS primer sets to chromosomes in wheat and barley to determine the potential transferability of the primer sets and the degree of correspondence between RFLP and STS locations. STS products mapped to the same chromosome group in wheat and barley 75% of the time. RFLP location predicted S

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15

Vafin, R. R., Kh Kh Gilmanov, P. N. Shastin, and A. V. Supova. "Modeling of PCR-RFLP genotyping of cattle by polymorphic markers of iNOS gene." Agrarian science, no. 2 (February 20, 2024): 66–70. http://dx.doi.org/10.32634/0869-8155-2024-379-2-66-70.

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Relevance. The study of the Bos taurus iNOS gene polymorphism and its association with resistance to bovine leukemia, as well as with breeding value in terms of milk productivity, is a topical subject of genetic selection research.The purpose of this study was to identify and map polymorphic restriction sites in 4 SNP markers (AH13-1, AH13-2, AH13-3, AH13-4) of the Bos taurus iNOS gene, followed by PCR-RFLP profiling of the encountered genotypes and modeling of the method of gene testing of cattle by the listed polymorphic markers of the analyzed locus.Methods. An effective tool for visualizin

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16

Kremer, K., D. van Soolingen, R. Frothingham, et al. "Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility." Journal of Clinical Microbiology 37, no. 8 (1999): 2607–18. http://dx.doi.org/10.1128/jcm.37.8.2607-2618.1999.

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In this study, the currently known typing methods forMycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typing methods and in seven PCR-based assays. In all methods, one or more repetitive DNA elements were targeted. The strain typing and the DNA fingerprint analysis were performed in the laboratory most experienced in the respective method. To examine intralaboratory reproducibility, b

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17

Nuryanto, Agus, Rani Eva Dewi, and Hendro Pramono. "Genetic Homogenity of Commerson’s Anchovy (Stolephorus commersonnii) in Segara Anakan Cilacap Central Java Inferred from PCR-RFLP Markers." Biogenesis: Jurnal Ilmiah Biologi 7, no. 1 (2019): 14. http://dx.doi.org/10.24252/bio.v7i1.6352.

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Commerson’s anchovy (Stolephorus commersonnii) is a small pelagic fish that live in a group and its existence is very abundant in Segara Anakan Cilacap. This anchovy is widely consumed by communities live around Segara Anakan. This leads to a high exploitation rate. Exploited populations generally have low genetic diversity. This study aims to evaluate genetic diversity of commerson’s anchovy population in Segara Anakan Cilacap inferred from PCR-RFLP of the cytochrome c oxidase 1 (CO1) gene. This study was conducted from January to April 2018 and used survey method by applying random sampling.

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18

Maciel, Danielli Barreto, Lílian Vieira de Medeiros, Vivian Vieira de Medeiros, Mariele Porto Carneiro Leão, Luis Eduardo Aranha Camargo, and Neiva Tinti de Oliveira. "[NO TITLE AVAILABLE]." Brazilian Archives of Biology and Technology 53, no. 6 (2010): 1255–66. http://dx.doi.org/10.1590/s1516-89132010000600001.

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Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative

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Shevchenko, Ye, and K. Kopylov. "Genotyping of New Zealand White Rabbits by PCR-RFLP Markers." Agricultural Science and Practice 2, no. 2 (2015): 21–25. http://dx.doi.org/10.15407/agrisp2.02.021.

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Aim. To investigate the genetic structure of New Zealand white rabbits population by different types of DNA- markers. Methods. The individual genotypes of animals were identifi ed using the polymerase chain reaction with further determination of the restriction fragment length polymorphism (RFLP-analysis). Results. The data on the distribution of allele variants of molecular markers in the population of rabbits were obtained; the impact of the genotype factor on meat production, prolifi cacy and milk production traits was determined. The relationship between genotypes by polymorphic DNA-marker

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20

Saal, B., and Günter Wricke. "Development of simple sequence repeat markers in rye (Secale cereale L.)." Genome 42, no. 5 (1999): 964–72. http://dx.doi.org/10.1139/g99-052.

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Simple sequence repeats (SSRs), also referred to as microsatellites, represent a PCR-based marker system that has been described in mammalian and plant genomes in recent years. In self-pollinating crop plants they have been shown to be superior to other DNA markers with respect to their level of polymorphism. The technical advantages compared with RFLP markers should also facilitate marker analysis in outcrossing crops like rye. In order to determine the usefulness of SSR markers in rye genetics and breeding, several genomic libraries were screened for (CT/GA)n and (GT/CA)n dinucleotide repeat

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Santoso, Panca J., Ghizan B. Saleh, Norihan M. Saleh, and Suhaimi Napis. "PHYLOGENETIC RELATIONSHIPS AMONGST 10 Durio SPECIES BASED ON PCR-RFLP ANALYSIS OF TWO CHLOROPLAST GENES." Indonesian Journal of Agricultural Science 6, no. 1 (2013): 20. http://dx.doi.org/10.21082/ijas.v6n1.2005.20-27.

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Twenty seven species of Durio have been identified in Sabah and Sarawak, Malaysia, but their relationships have not been studied. This study was conducted to analyse phylogenetic relationships amongst 10 Durio species in Malaysia using PCR-RFLP on two chloroplast DNA genes, i.e. ndhC-trnV and rbcL. DNAs were extracted from young leaves of 11 accessions from 10 Durio species collected from the Tenom Agriculture Research Station, Sabah, and University Agriculture Park, Universiti Putra Malaysia. Two pairs of oligonucleotide primers, N1-N2 and rbcL1-rbcL2, were used to flank the target regions nd

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Santoso, Panca J., Ghizan B. Saleh, Norihan M. Saleh, and Suhaimi Napis. "PHYLOGENETIC RELATIONSHIPS AMONGST 10 Durio SPECIES BASED ON PCR-RFLP ANALYSIS OF TWO CHLOROPLAST GENES." Indonesian Journal of Agricultural Science 6, no. 1 (2013): 20. http://dx.doi.org/10.21082/ijas.v6n1.2005.p20-27.

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Twenty seven species of Durio have been identified in Sabah and Sarawak, Malaysia, but their relationships have not been studied. This study was conducted to analyse phylogenetic relationships amongst 10 Durio species in Malaysia using PCR-RFLP on two chloroplast DNA genes, i.e. ndhC-trnV and rbcL. DNAs were extracted from young leaves of 11 accessions from 10 Durio species collected from the Tenom Agriculture Research Station, Sabah, and University Agriculture Park, Universiti Putra Malaysia. Two pairs of oligonucleotide primers, N1-N2 and rbcL1-rbcL2, were used to flank the target regions nd

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23

Blum, Eyal, Kede Liu, Michael Mazourek, Eun Young Yoo, Molly Jahn, and Ilan Paran. "Molecular mapping of the C locus for presence of pungency in Capsicum." Genome 45, no. 4 (2002): 702–5. http://dx.doi.org/10.1139/g02-031.

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Pungency owing to the presence of capsaicinoids is a unique character of pepper (Capsicum spp.). Capsaicinoids are produced in the placenta and it has long been known that a single dominant gene, C, is required for pungent genotypes to produce capsaicinoids. We mapped C to pepper chromosome 2 in a cross between a pungent Capsicum frutescens wild accession and a non-pungent Capsicum annuum bell pepper. This position confirmed results from earlier studies. The RFLP marker TG 205 cosegregated with C and two additional RFLP markers were also located within 1 cM. The recessive allele at the C locus

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24

Soto, A. "Simple PCR Markers for the Study of Chloroplast in Eucalyptus." Silvae Genetica 53, no. 1-6 (2004): 139–40. http://dx.doi.org/10.1515/sg-2004-0025.

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Summary Combined use of two newly designed PCR primers with already described rpl2 and trnH primers, yields amplification of three non-independent products from the hypervariable JLA region of eucalypt chloroplast. Polymorphism analysis of the resulting PCR markers is proved to be a time- and cost-efficient alternative to traditional cpDNA techniques as RFLP or sequencing for Eucalyptus globulus Labill. population genetics studies.

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Ussenbekov, Yessengali, Aigerim Bagdat, Zhanat Bimenova, et al. "Identification of monomorphic and polymorphic genes associated with recessive fertility defects in Holstein cows reared in Kazakhstan." Veterinarski arhiv 92, no. 1 (2022): 27–35. http://dx.doi.org/10.24099/vet.arhiv.139.

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Haplotypes of candidate genes namely: apoptotic protease activating factor 1 (APAF1 p.Q579X or HH1), glycinamide ribonucleotide formyltransferase (GART or HH4), structural maintenance of chromosomes 2 (SMC2 or HH3), and haplotype cholesterol deficiency (HCD) genes associated with recessive fertility defects (loss of fertility) were investigated in imported Canadian Holstein cows reared at “Medeu Commerce” LLP breeding farm in Kazakhstan. The genotypic profiling of the APAF1/HH1, GART/HH4 fertility haplotype carriers was carried out by PCR-RFLP methods using BstC8I and Tru9I and MseI, while the

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26

Hausner, G., K. Y. Rashid, E. O. Kenaschuk, and J. D. Procunier. "The development of codominant PCR/RFLP based markers for the flax rust-resistance alleles at the L locus." Genome 42, no. 1 (1999): 1–8. http://dx.doi.org/10.1139/g98-101.

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The flax L locus exists as a single gene with at least 13 alleles with different rust-resistance specificities. With regards to resistance to North American races of flax rust the L2, L6, and L11 alleles are of major importance. Molecular markers have been developed by screening primer sets, whose sequences were based on the nucleotide sequence of L6, for their ability to amplify segments of the L gene. One primer combination was found to amplify only the L6 or L11 alleles and another primer set was found to amplify the 3' end of all important L alleles. The latter primer set yielded a 1.3 kb

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Witsenboer, H., R. W. Michelmore, and J. Vogel. "Identification, genetic localization, and allelic diversity of selectively amplified microsatellite polymorphic loci in lettuce and wild relatives (Lactuca spp.)." Genome 40, no. 6 (1997): 923–36. http://dx.doi.org/10.1139/g97-119.

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Selectively amplified microsatellite polymorphic locus (SAMPL) analysis is a method of amplifying microsatellite loci using generic PCR primers. SAMPL analysis uses one AFLP primer in combination with a primer complementary to microsatellite sequences. SAMPL primers based on compound microsatellite sequences provided the clearest amplification patterns. We explored the potential of SAMPL analysis in lettuce to detect PCR-based codominant microsatellite markers. Fifty-eight SAMPLs were identified and placed on the genetic map. Seventeen were codominant. SAMPLs were dispersed with RFLP markers o

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Buczkowska, Katarzyna. "Application of PCR – RFLP markers for identification of genetically delimited groups of the Calypogeia fissa complex (Jungermanniopsida, Calypogeiaceae)." Biodiversity Research and Conservation 38, no. 1 (2015): 1–8. http://dx.doi.org/10.1515/biorc-2015-0009.

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AbstractCurrently, two subspecies are formally recognized within Calypogeia fissa: C. fissa subsp. fissa occurring in Europe and C. fissa subsp. neogea known from North America. Genetic studies have revealed a complex structure of this species. Within the European part of distribution, three genetically distinct groups PS, PBand G are distinguished. The combination of the SCAR marker Cal04 and PCR-RFLP markers with three restriction enzymes (SmaI, TaqI and TspGWI) allowed the recognition of all groups within the C. fissa complex. The TaqI enzyme recognizing the restriction sites in the PCR pro

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Gong, Wei, Cheng-Xin Fu, Yu-Ping Luo, and Ying-Xiong Qiu. "Molecular Identification ofSinopodophyllum hexandrumandDysosmaSpecies using cpDNA Sequences and PCR-RFLP Markers." Planta Medica 72, no. 07 (2006): 650–52. http://dx.doi.org/10.1055/s-2006-931535.

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Fang, D. Q., C. T. Federici, and M. L. Roose. "A High-Resolution Linkage Map of the Citrus Tristeza Virus Resistance Gene Region in Poncirus trifoliata (L.) Raf." Genetics 150, no. 2 (1998): 883–90. http://dx.doi.org/10.1093/genetics/150.2.883.

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Abstract Resistance to citrus tristeza virus (CTV) was evaluated in 554 progeny of 10 populations derived from Poncirus trifoliata. A dominant gene (Ctv) controlled CTV resistance in P. trifoliata. Twenty-one dominant PCR-based DNA markers were identified as linked to Ctv by bulked segregant analysis. Of the 11 closest markers to Ctv, only 2 segregated in all populations. Ten of these markers were cloned and sequenced, and codominant RFLP markers were developed. Seven RFLP markers were then evaluated in 10 populations. Marker orders were consistent in all linkage maps based on data of single p

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Prioli, R. A., R. A. Curi, L. A. Chardulo, V. N. Gomes, S. M. A. P. Prioli, and M. D. S. Mota. "Characterization of gene polymorphisms related to immune system physiology in Mangalarga horses." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 64, no. 5 (2012): 1302–8. http://dx.doi.org/10.1590/s0102-09352012000500030.

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The objectives of this study were to standardize a PCR-RFLP genotyping method for the AY_731081:g.1900T>C SNP of the equine CD14 gene, and to characterize this SNP and two other polymorphisms (AY_005808: c.1530A>G of the TLR4 gene and AX_463789: g.133T>C of the Cε gene) in Mangalarga horses, in order to contribute to future studies investigating the association between DNA markers and traits related to immune system physiology in this breed. A total of 151 Mangalarga horses of both sexes and variable ages, representative of the population of São Paulo State, were used. PCR-RFLP was fo

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Alipanah, M., A. Torkamanzehi, and H. Taghavi. "Sex determination in ostrich (Struthio camelus) using DNA markers." Canadian Journal of Animal Science 90, no. 3 (2010): 357–60. http://dx.doi.org/10.4141/cjas09125.

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Production of bird species such as ostrich (Struthio camelus) has been gaining increasing importance in Iran as well as many other countries. Ostrich, similar to many other species of birds, lacks sexual dimorphism, making it difficult to differentiate between males and females, especially at an early age, which can be problematic in breeding programs. Recently developed molecular genetic methods that utilize polymerase chain reaction (PCR) based techniques can facilitate rapid identification of the bird’s sex in these species using a DNA sample, which can be easily extracted from blood or fea

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ONISCHENKO, O. N. "GENETIC POLYMORPHISM OF THE GH, GDF9 GENES IN RUSSIAN MEAT MERINO SHEEP BREED." Sheep, goats, wool business, no. 2 (2023): 14–17. http://dx.doi.org/10.26897/2074-0840-2023-2-14-17.

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The article presents the results of PCR-RFLP analysis of the genotype distribution of the GH and GDF9 genes in sheep of the Russian meat merino breed (n = 100), bred in the conditions of the Stavropol Territory. The most popular are genetic markers that are interconnected with candidate genes, the protein products of which play a signifi cant role in the formation or regulation of physiological and biochemical processes. Using the PCR-RFLP methods, the specifi city of the allelic spectrum of the somatotropin and differential growth factor genes was established.

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Вафин, Р. Р., И. Ю. Михайлова, И. И. Агейкина, and Л. Н. Харламова. "Predictive model for tea varietal identification by PCR-RFLP analysis of Camellia sinensis SNP markers." Food processing industry, no. 1 (January 7, 2024): 74–77. http://dx.doi.org/10.52653/ppi.2024.1.1.014.

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Растение вида Camellia sinensis является основным сырьём для производства чайной продукции. Оценка подлинности чайного сырья и готовой продукции может быть осуществлена сортовой идентификацией чая молекулярно-генетическими методами исследования, имеющими широкий арсенал диагностических подходов, в том числе к детекции однонуклеотидных замен (SNP – Single Nucleotide Polymorphism) анализом полиморфизма длин рестрикционных фрагментов ДНК, предварительно амплифицированных полимеразной цепной реакцией (ПЦР-ПДРФ). Цель настоящего исследования заключалась в выявлении и картировании полиморфных сайтов

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Clark, T. L., L. J. Meinke, and J. E. Foster. "PCR–RFLP of the mitochondrial cytochrome oxidase (subunit I) gene provides diagnostic markers for selected Diabrotica species (Coleoptera: Chrysomelidae)." Bulletin of Entomological Research 91, no. 6 (2001): 419–27. http://dx.doi.org/10.1079/ber2001130.

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AbstractAdult and larval identification of Diabrotica can be difficult. Some adult identifications require considerable taxonomic experience while larvae of many Diabroticaspecies are morphologically indistinguishable. This study was conducted to determine whether 12 pest and non-pest Diabrotica species could be separated using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). A 1308 bp portion of the mitochondrial cytochrome oxidase subunit I gene (COI) was amplified using PCR and digested using several restriction endonucleases. Double digests of COI amplicons wi

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Weissensteiner, Th, and J. S. Lanchbury. "An Integrated Multiplex-PCR and PCR-RFLP Typing System for Markers Associated with Seronegative Arthritides." Human Immunology 59, no. 2 (1998): 119–32. http://dx.doi.org/10.1016/s0198-8859(97)00260-7.

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Elkady, Fathy M., Abdulaziz A. Al-Askar, Ahmed Abdel Tawab, Mohammad M. Alkherkhisy, Amr A. Arishi, and Amr H. Hashem. "Comparative Genotypic Analysis of RAPD and RFLP Markers for Molecular Variation Detection of Methicillin-Resistant Staphylococcus aureus Clinical Isolates." Medicina 58, no. 9 (2022): 1245. http://dx.doi.org/10.3390/medicina58091245.

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Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) isolates are associated with various diseases ranged from mild superficial impairments to invasive infections. This study aimed to evaluate the ability of polymerase chain reaction (PCR) based methods namely, restriction fragment length polymorphism (RFLP) of the coa gene and random amplified polymorphic DNA (RAPD), to determine the genetic diversity of MRSA isolates. Materials and Methods: A total of 37 MRSA isolates were conventionally identified depending on their biochemical and microbiological culture characteri

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Budakva, Yelyzaveta, Konstantin Pochernyaev, and Artem Pochernyaev. "BIOLOGICAL SAMPLES CONTAMINATION CONTROL OF THE SUS SCROFA USING HAPLOID DNA MARKERS." Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine, no. 129 (2023): 70–78. http://dx.doi.org/10.32900/2312-8402-2023-129-70-78.

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This paper proposes an effective method for controlling the contamination of biological samples of Sus scrofa with alien material in the preanalytical phase of a PCR study. Because PCR is highly sensitive, even a small amount of DNA containing alien biological substances can lead to false results. In the case of analysis of contaminated biological samples using diploid DNA markers, a mixture of two different homozygotes will be defined as a heterozygote. Unlike diploid DNA markers, a mixture of two different haplotypes is uniquely determined. To perform the study in the slaughter shop of the G

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Forsström, Per-Olov, Robert Koebner, and Arnulf Merker. "The conversion of wheat RFLP probes into STS markers via the single-stranded conformation polymorphism technique." Genome 46, no. 1 (2003): 19–27. http://dx.doi.org/10.1139/g02-101.

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We describe a flexible and general strategy for converting a wheat RFLP-based assay into a PCR-based sequence-tagged site (STS), and have applied it to derive markers for a powdery mildew resistance gene present in a wheatrye translocation. The concept is based on deriving PCR primers that amplify all of the homoeoloci defined by a single-copy cDNA sequence, and separating the resulting mixture of homoeoamplicons via single-stranded conformation polymorphism (SSCP) gels, which are able to detect minor differences between related DNA sequences. After their separation, the individual homoeoampl

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Garland, S. H. "The assessment of TGGE for the detection of interspecific and intergeneric DNA-marker polymorphism within Solanaceae." Australian Journal of Agricultural Research 56, no. 3 (2005): 291. http://dx.doi.org/10.1071/ar04141.

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RFLP markers are currently the most appropriate marker system for the identification of uncharacterised polymorphism at the interspecific and intergeneric level. Given the benefits of a PCR-based marker system and the availability of sequence information for many Solanaceous cDNA clones, it is now possible to target conserved fragments, for primer development, that flank sequences possessing interspecific polymorphism. The potential outcome is the development of a suite of markers that amplify widely in Solanaceae. Temperature gradient gel electrophoresis (TGGE) is a relatively inexpensive gel

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Merchant-Patel, Shreema, Patrick J. Blackall, Jillian Templeton, et al. "Campylobacter jejuni and Campylobacter coli Genotyping by High-Resolution Melting Analysis of a flaA Fragment." Applied and Environmental Microbiology 76, no. 2 (2009): 493–99. http://dx.doi.org/10.1128/aem.01164-09.

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ABSTRACT The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jejuni and Campylobacter coli. High-resolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The objective of this study was to apply HRM analysis to flaA-based genotyping. The initial aim was to identify a suitable flaA fragment. It was found that the PCR primers commonly used to amplify the flaA short variable repeat (SVR) yielded a mixed PCR product unsuitable for HRM analysis. However, a PCR primer set composed of the ups

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Kritsiriwuthinan, Kanyanan, Warunee Ngrenngarmlert, Rapatbhorn Patrapuvich, Supaksajee Phuagthong та Kantima Choosang. "Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP". Journal of Tropical Medicine 2023 (11 серпня 2023): 1–8. http://dx.doi.org/10.1155/2023/8855171.

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Considering the importance of merozoite surface proteins (MSPs) as vaccine candidates, this study was conducted to investigate the polymorphism and genetic diversity of Plasmodium vivax merozoite surface protein 3-alpha (PvMSP-3α) in Thailand. To analyze genetic diversity, 118 blood samples containing P. vivax were collected from four malaria-endemic areas in western and southern Thailand. The DNA was extracted and amplified for the PvMSP-3α gene using nested PCR. The PCR products were genotyped by PCR-RFLP with Hha I and Alu I restriction enzymes. The combination patterns of Hha I and Alu I R

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Burnens, A. P., J. Wagner, H. Lior, J. Nicolet, and J. Frey. "Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli." Epidemiology and Infection 114, no. 3 (1995): 423–31. http://dx.doi.org/10.1017/s0950268800052134.

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SUMMARYSeveral typing systems have been described forCampylobacter jejuniandC. coli, to assess the complex epidemiology of these important enteric pathogens. In the present study two typing methods, slide agglutination according to the Lior scheme, and the demonstration of restriction-fragment length polymorphisms (RFLP) of flagellar genes, have been used in parallel on a set of 194 strains. This set comprised 118 sero-reference strains ofC. jejuniandC. coliof the Lior scheme, as well as 76 clinical isolates. All isolates were serotyped and subjected to PCR for amplification of flagellar genes

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Biriukova, O. D., T. M. Suprovych, M. P. Suprovych, S. V. Laiter-Moskaliuk, and I. O. Chornyi. "FEATURES OF DIAGNOSIS OF NECROBACTERIOSIS OF COWS BY PCR-RFLP." Podilian Bulletin: Agriculture, Engineering, Economics, no. 32 (June 29, 2020): 26–37. http://dx.doi.org/10.37406/2706-9052-2020-1-3.

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Molecular genetic markers can detect polymorphism at the DNA level. This feature determines the possibility of their widespread use in genetics and breeding. Alleles of the BoLA-DRB3 gene (exon 2) can act as such markers if a statically significant association between the disease and the allele is established. The presence of such DNA markers in the genotype of animals makes it possible to judge the likelihood of disease in postnatal ontogenesis immediately after the birth of a heifer, based on which we can conclude about the conditions of further use of the animal in the main herd. According

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Nuroniah, Hani Sitti, Oliver Gailing, and Reiner Finkeldey. "Development of a diagnostic DNA marker for the geographic origin of Shorea leprosula." Holzforschung 71, no. 1 (2017): 1–10. http://dx.doi.org/10.1515/hf-2016-0086.

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Abstract The development of sequence characterized amplified region (SCAR) markers derived from amplified fragment length polymorphisms (AFLPs) is described for Shorea leprosula. An AFLP fragment that showed nearly complete differentiation between Borneo and Sumatra was gel-extracted, sequenced, and converted into a SCAR marker using the inverse polymerase chain reaction (PCR) technique. The single nucleotide polymorphism (SNP) that originally caused the AFLP was found in the MseI restriction site. Differentiation between islands was detected either as size variation of the codominant SCAR mar

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Ekawasti, Fitrine, Eko Setyo Purwanto, Farlin Nepho, et al. "The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker." HAYATI Journal of Biosciences 30, no. 4 (2023): 725–33. http://dx.doi.org/10.4308/hjb.30.4.725-733.

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Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii R

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Lu, Xin-Sheng, Jing Li, Chen Wang, et al. "Molecular Prevalence and Genotyping of Toxoplasma gondii in Sheep Tissues Intended for Human Consumption in Shanxi Province, North China." Animals 15, no. 12 (2025): 1685. https://doi.org/10.3390/ani15121685.

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Toxoplasma gondii is one of the most widely distributed intracellular parasites worldwide, which can infect humans and a wide range of warm-blooded animals including sheep, with felines serving as its definitive host. T. gondii infection in sheep can lead to premature births, abortions and stillbirths, causing significant economic losses to the sheep industry. Sheep farming has become a key pillar of the agricultural economy in Shanxi Province, North China, but little is known about T. gondii infection in sheep in this province. In the present study, a total of 755 sheep tissue samples (682 mu

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Pal, Narinder, Jagdeep S. Sandhu, Leslie L. Domier, and Frederic L. Kolb. "Development and Characterization of Microsatellite and RFLP-Derived PCR Markers in Oat." Crop Science 42, no. 3 (2002): 912. http://dx.doi.org/10.2135/cropsci2002.0912.

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Pal, Narinder, Jagdeep S. Sandhu, Leslie L. Domier, and Frederic L. Kolb. "Development and Characterization of Microsatellite and RFLP‐Derived PCR Markers in Oat." Crop Science 42, no. 3 (2002): 912–18. http://dx.doi.org/10.2135/cropsci2002.9120.

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DOCKER, M. F., G. R. HAAS, D. H. GOODMAN, S. B. REID, and D. D. HEATH. "PCR-RFLP markers detect 29 mitochondrial haplotypes in Pacific lamprey (Entosphenus tridentatus)." Molecular Ecology Notes 7, no. 2 (2006): 350–53. http://dx.doi.org/10.1111/j.1471-8286.2006.01605.x.

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