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Journal articles on the topic 'PCR-RFLP'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Karaca, Mehmet, Ayse Gul Ince, Saadet Tugrul Ay, Kenan Turgut, and Ahmet Naci Onus. "PCR-RFLP and DAMD-PCR genotyping forSalviaspecies." Journal of the Science of Food and Agriculture 88, no. 14 (2008): 2508–16. http://dx.doi.org/10.1002/jsfa.3372.

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2

VICTOIR, K., A. L. BAÑULS, J. AREVALO, et al. "The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia." Parasitology 117, no. 1 (1998): 1–13. http://dx.doi.org/10.1017/s0031182098002789.

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In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a refe

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3

Hazlianda, Cut, Kamaliah Muis, and Isma Lubis. "A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris." Open Access Macedonian Journal of Medical Sciences 5, no. 7 (2017): 844–47. http://dx.doi.org/10.3889/oamjms.2017.197.

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BACKGROUND: Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results.AIM: This study aimed to find whether the PCR-RFLP test could yield the same fungal species result as a fungal culture.METHODS: The specimens were skin scrapings from thirty-one patients suspected tinea cruris

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Bouzouita, Imen, Henda Draoui, Samia Mahdhi, Leila Essalah, and Leila Slim Saidi. "Evaluation of PCR pncA-restriction fragment length polymorphism and PCR amplification of genomic regions of difference for the identification of M. bovis strains in lymph nodes cultures." African Health Sciences 21, no. 3 (2021): 985–89. http://dx.doi.org/10.4314/ahs.v21i3.4.

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Background: A rapid accurate identification of Mycobacterium bovis is essential for surveillance purposes. Objectives: A PCR pncA-Restriction Fragment Length Polymorphism (RFLP) and a multiplex PCR based on the detection of 3 regions of difference (RD-PCR): RD9, RD4 and RD1 were evaluated for the identification of M. bovis in lymph nodes cultures, in Tunisia, during 2013-2015. Methods: Eighty-two M. tuberculosis complex strains were identified using the biochemical tests, GenoType MTBC assay, PCR pncA-RFLP and RD-PCR. Results: The PCR pncA-RFLP showed that 54 M. bovis strains

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5

Khayyira, Amalia Sitti, Viktoria Mardhika Estepane, and Amarila Malik. "RAPID PCR–BASED DETECTION OPTIMIZATION OF PORCINE DNA IN GELATIN CAPSULE SHELL." International Journal of Applied Pharmaceutics 10, no. 6 (2018): 217. http://dx.doi.org/10.22159/ijap.2018v10i6.29346.

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Objective: This study was conducted to optimize the genomic deoxyribonucleic acid (DNA) based molecular detection of gelatin derived from porcine by performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and duplex PCR method employing cyt B gene.Methods: Optimization was carried out for DNA extraction, PCR conditions, and the sensitivity of the PCR-RFLP method. Due to the very low DNA trace in gelatin after the various manufacturing process, the extraction was optimized to obtain sufficient DNA which was visible on the agarose gel. PCR-RFLP was carried out us

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6

Andrini, Fauzia, Imam Supardi, Sunarjati Sudigdoadi, and Sadeli Masria. "Detection of Staphylococcus aureus’s Strain Similarity on Surgical Ward Nurses’s Hand and Nose and Post Operative Wound Infection Using Coa Gene Through PCR-RFLP Method." Jurnal Ilmu Kedokteran 4, no. 2 (2017): 116. http://dx.doi.org/10.26891/jik.v4i2.2010.116-122.

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Staphylococcus aureus (S.aureus) remains to be the most important cause of post operative wound infection. Nursescould become reservoirs to transmit S.aureus through contaminated hands transiently, or through colonized nose.Strain polymorphism could be determined by Restriction Fragment Length Polymorphism (RFLP), using coa gene andrestriction endonuclease enzyme Alu1. There were 30 isolates of S.aureus’s infection, and 20 isolates taken from handsand nose of the nurses in charge. From 50 isolate positive S.aureus, PCR results showed single and multiple bandswithin 300 to 900 base pairs (bp) i

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7

Lueders, Tillmann, and Michael W. Friedrich. "Evaluation of PCR Amplification Bias by Terminal Restriction Fragment Length Polymorphism Analysis of Small-Subunit rRNA and mcrA Genes by Using Defined Template Mixtures of Methanogenic Pure Cultures and Soil DNA Extracts." Applied and Environmental Microbiology 69, no. 1 (2003): 320–26. http://dx.doi.org/10.1128/aem.69.1.320-326.2003.

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ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) analysis is a widely used method for profiling microbial community structure in different habitats by targeting small-subunit (SSU) rRNA and also functional marker genes. It is not known, however, whether relative gene frequencies of individual community members are adequately represented in post-PCR amplicon frequencies as shown by T-RFLP. In this study, precisely defined artificial template mixtures containing genomic DNA of four different methanogens in various ratios were prepared for subsequent T-RFLP analysis. PCR amplic

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8

Hubáček, Jaroslav A., H. Pikhart, A. Peasey, R. Kubínová, and M. Bobák. "Nobody Is Perfect: Comparison of the Accuracy of PCR-RFLP and KASP™ Method for Genotyping. ADH1B and FTO Polymorphisms as Examples." Folia Biologica 61, no. 4 (2015): 156–60. http://dx.doi.org/10.14712/fb2015061040156.

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DNA genotyping is among the most common analyses currently performed in scientific research. Two high-throughput genotyping techniques are widely used – the “classic” PCR-RFLP and probe-based methods such as TaqMan® PCR assay or KASP™ genotyping. The probe-based techniques are claimed to be more accurate than PCR-RFLP; however, the evidence for this claim is sparse. We have directly compared results of genotyping of two SNPs (rs1229984 and rs17817449) obtained by the PCR-RFLP and KASP™ in 1,502 adult Caucasians. The results were identical in 97.3 % and 95.9 % cases, respectively. Discrepancies

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9

Rousseaux, S., M. Olier, J. P. Lemaître, P. Piveteau, and J. Guzzo. "Use of PCR-Restriction Fragment Length Polymorphism of inlA for Rapid Screening of Listeria monocytogenes Strains Deficient in the Ability To Invade Caco-2 Cells." Applied and Environmental Microbiology 70, no. 4 (2004): 2180–85. http://dx.doi.org/10.1128/aem.70.4.2180-2185.2004.

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ABSTRACT A PCR-restriction fragment length polymorphism (RFLP) method was developed in order to screen a large number of strains for impaired adhesion to epithelial cells due to expression of truncated InlA. inlA polymorphism was analyzed by PCR-RFLP in order to correlate inlA PCR-RFLP profiles and production of truncated InlA. Thirty-seven Listeria monocytogenes strains isolated from various sources, including five noninvasive and two invasive reference strains, were screened. Two endonucleases (AluI and Tsp509I) were used, and they generated five composite profiles. Thirteen L. monocytogenes

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Walker, Elaine S., Robert A. Preston, J. Christopher Post, Garth D. Ehrlich, John H. Kalbfleisch, and Karin L. Klingman. "Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method." Journal of Clinical Microbiology 36, no. 7 (1998): 1977–83. http://dx.doi.org/10.1128/jcm.36.7.1977-1983.1998.

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Moraxella (Branhamella)catarrhalis, a causative agent of otitis media, sinusitis, and exacerbation of bronchitis, has acquired widespread ability to produce β-lactamase and can be nosocomially transmitted. The typing methods used in epidemiological analyses of M. catarrhalisare not optimal for genetic analyses. Two methods, a multiple-locus Southern blot (SB) method and a single-locus PCR-restriction fragment length polymorphism (RFLP) method, were developed and used to assess genetic diversity and potential clinical and geographic relationships in M. catarrhalis. Nine randomly cloned M. catar

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11

BUI, Thi Thuy Nga, Thi Thanh Huyen NGUYEN, Anh Ngọc NGUYEN, and Quang Binh TRAN. "<span>DEVELOPMENT OF A RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD FOR GENOTYPING THE <em>ATP2B1</em> RS1801133 POLYMORPHISM IN VIETNAMESE POPULATION</span>." Tạp chí Dinh dưỡng và Thực phẩm 20, no. 3E (2024): 72–80. http://dx.doi.org/10.56283/1859-0381/661.

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Aims: The rs2681492, a single nucleotide polymorphism in the ATP2B1 gene, has been reported to be associated with cardiovascular diseases and traits including blood pressure, chronic heart failure, and hypertension. The study aimed to develop a PCR-RFLP genotyping method for rs2681492 among the Vietnamese population. Methods and Results: The Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method incorporated optimizations of primers, thermal cycles, and restriction enzymes in a novel restriction fragment length polymorphism (RFLP) approach. To evaluate the accurac

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12

Sola, Christophe, Lionel Horgen, Jerome Maïsetti, Anne Devallois, Khye Seng Goh, and Nalin Rastogi. "Spoligotyping Followed by Double-Repetitive-Element PCR as Rapid Alternative to IS6110 Fingerprinting for Epidemiological Studies of Tuberculosis." Journal of Clinical Microbiology 36, no. 4 (1998): 1122–24. http://dx.doi.org/10.1128/jcm.36.4.1122-1124.1998.

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A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110–PGRS (polymorphic GC-rich region)–PCR, also designated double-repetitive-element PCR (DRE-PCR). During the first phase of this investigation, 72 clinical strains representing 52 patients were initially typed by IS6110-restriction fragment length polymorphism (RFLP) and DR-RFLP, followed by spoligotyping and DRE-PCR. In the second phase of this investigation, the discriminating ability of spoligotyping plus DRE-PCR was

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13

Ivona Djurkin, Kušec, Samac Danijela, Margeta Vladimir, Radišić Žarko, Vincek Dragutin, and Kušec Goran. "Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages." Czech Journal of Food Sciences 35, No. 5 (2017): 386–91. http://dx.doi.org/10.17221/243/2016-cjfs.

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The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in the amount of 1 and 5%. DNA was extracted from five regions of each sausage and PCR-RFLP together with PCR using species-specific primers was performed. PCR-RFLP analysis was successful only for chicken meat, while species-specific PCR was effective for identification of chicken, eef and sheep meat in all ratios and from all regions of the sau

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14

Trejaut, Jean, and Heather Dunckley. "HLA-DRB5 genotyping by PCR-RFLP." Tissue Antigens 43, no. 1 (1994): 60–63. http://dx.doi.org/10.1111/j.1399-0039.1994.tb02300.x.

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15

MacAllister, Thomas W. "{BLR 2083} DNA Fingerprinting - PCR - RFLP." Biotechnology Law Report 14, no. 4 (1995): 641–48. http://dx.doi.org/10.1089/blr.1995.14.641a.

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16

Pourzand, Charareh, and Peter Cerutti. "Genotypic mutation analysis by RFLP/PCR." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 288, no. 1 (1993): 113–21. http://dx.doi.org/10.1016/0027-5107(93)90213-y.

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17

Montoro, Ernesto, José Valdivia, and Sylvia Cardoso Leão. "Molecular Fingerprinting of Mycobacterium tuberculosisIsolates Obtained in Havana, Cuba, by IS6110 Restriction Fragment Length Polymorphism Analysis and by the Double-Repetitive-Element PCR Method." Journal of Clinical Microbiology 36, no. 10 (1998): 3099–102. http://dx.doi.org/10.1128/jcm.36.10.3099-3102.1998.

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Mycobacterium tuberculosis sputum isolates from 38 patients, obtained in the first 6 months of 1997 in Havana, Cuba, were characterized by IS6110 restriction fragment length polymorphism (RFLP) analysis and the double-repetitive-element PCR (DRE-PCR) method. Among 41 strains from 38 patients, 24 and 25 unique patterns, and 5 and 4 cluster patterns, were found by the RFLP and DRE-PCR methods, respectively. Patients within two of these clusters were found to be epidemiologically related, while no relation was observed in patients in the other clusters. The DRE-PCR method is rapid, and it was as

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18

Tripathi, Giri Raj. "A Simplified and Cheapest Method for the Diagnosis of Sickle Cell using Whole Blood PCR and RFLP in Nepal." Tribhuvan University Journal 30, no. 2 (2016): 57–64. http://dx.doi.org/10.3126/tuj.v30i2.25547.

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Sickle cell anemia is a serious genetic health problem dominated in Tharu community of western Nepal. Molecular methods like PCR and RFLP are the best method to identify Sickle c ell anemia trait. Molecular analysis needed many steps and expensive chemicals and Kits. The aim of this research was to develop a simple and cheapest method to process from whole blood sample for the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents and Kits. In this study molecular identification of sickle cell traits is subjects using whole blo

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19

Sellyei, Boglárka, Éva Ivanics, and Tibor Magyar. "Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene." Acta Veterinaria Hungarica 61, no. 1 (2013): 1–8. http://dx.doi.org/10.1556/avet.2012.048.

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The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correla

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Zaczek, Anna, Anna Brzostek, Arkadiusz Wojtasik, Anna Sajduda, and Jaroslaw Dziadek. "Comparison of Ligation-Mediated PCR Methods in Differentiation ofMycobacterium tuberculosisStrains." BioMed Research International 2014 (2014): 1–4. http://dx.doi.org/10.1155/2014/782071.

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Fast and inexpensive identification of epidemiological links between limited number ofMycobacterium tuberculosisstrains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions. The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such initial, epidemiological analysis. Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR

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21

Graf, Joerg. "Diverse Restriction Fragment Length Polymorphism Patterns of the PCR-Amplified 16S rRNA Genes in Aeromonas veronii Strains and Possible Misidentification ofAeromonas Species." Journal of Clinical Microbiology 37, no. 10 (1999): 3194–97. http://dx.doi.org/10.1128/jcm.37.10.3194-3197.1999.

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Restriction fragment length polymorphism analysis after PCR amplification (RFLP-PCR) of the 16S rRNA gene has been previously proposed as a rapid method to identify Aeromonas species. In the present study, the precision of RFLP-PCR was evaluated with 62Aeromonas reference strains. The analysis revealed thatAeromonas veronii biovar sobria strains produce various patterns, possibly leading to its misidentification as an environmental species. For most other Aeromonas species little variation was noted. This study supports the usefulness of RFLP-PCR analysis to separate three clinically important

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Kindermann, Ellen Hochleitner Souza, Karoline Rodrigues Campos, and Adele Caterino-de-Araujo. "Performance of the tetra-primer PCR technique compared to PCR-RFLP in the search for rs12979860 (C/T) and rs8099917 (T/G) single nucleotide polymorphisms (SNPs) in the IFNL4 gene." Revista do Instituto Adolfo Lutz 82 (May 26, 2023): 1–12. http://dx.doi.org/10.53393/rial.2023.v82.39195.

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Single nucleotide polymorphisms (SNPs, rs12979860 e rs8099917) in the Interferon Lambda 4 gene (IFNL4, formerly IFNL3 and/or IL28B) has been associated with failure in the innate immune response, sustained virological response in hepatitis C, and HTLV-1-associated myelopathy (HAM) development. To search for these polymorphisms several methodologies can be employed, such as sequencing, real-time or quantitative polymerase chain reaction (qPCR), restriction fragment length polymorphism analysis in PCR products (PCR-RFLP), and tetra-primer PCR. The present study compared the performance of the te

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Kindermann, Ellen Hochleitner Souza, Karoline Rodrigues Campos, and Adele Caterino-de-Araujo. "Performance of the tetra-primer PCR technique compared to PCR-RFLP in the search for rs12979860 (C/T) and rs8099917 (T/G) single nucleotide polymorphisms (SNPs) in the IFNL4 gene." Revista do Instituto Adolfo Lutz 82 (May 26, 2023): 1–12. http://dx.doi.org/10.53393/rial.2023.v.82.39195.

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Single nucleotide polymorphisms (SNPs, rs12979860 e rs8099917) in the Interferon Lambda 4 gene (IFNL4, formerly IFNL3 and/or IL28B) has been associated with failure in the innate immune response, sustained virological response in hepatitis C, and HTLV-1-associated myelopathy (HAM) development. To search for these polymorphisms several methodologies can be employed, such as sequencing, real-time or quantitative polymerase chain reaction (qPCR), restriction fragment length polymorphism analysis in PCR products (PCR-RFLP), and tetra-primer PCR. The present study compared the performance of the te

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24

Hookey, John V., Judith F. Richardson, and Barry D. Cookson. "Molecular Typing of Staphylococcus aureus Based on PCR Restriction Fragment Length Polymorphism and DNA Sequence Analysis of the Coagulase Gene." Journal of Clinical Microbiology 36, no. 4 (1998): 1083–89. http://dx.doi.org/10.1128/jcm.36.4.1083-1089.1998.

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A typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resis

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25

Stubbs, Simon L. J., Jon S. Brazier, Paul R. Talbot, and Brian I. Duerden. "PCR-Restriction Fragment Length Polymorphism Analysis for Identification of Bacteroides spp. and Characterization of Nitroimidazole Resistance Genes." Journal of Clinical Microbiology 38, no. 9 (2000): 3209–13. http://dx.doi.org/10.1128/jcm.38.9.3209-3213.2000.

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Bacteroides spp. are opportunist pathogens that cause blood and soft tissue infections and are often resistant to antimicrobial agents. We have developed a combined PCR-restriction fragment length polymorphism (RFLP) technique to characterize the 16S rRNA gene for identification purposes and the nitroimidazole resistance (nim) gene for detection of resistance to the major antimicrobial agent used to treat Bacteroidesinfections: metronidazole (MTZ). PCR-RFLP analysis of 16S ribosomal (rDNA) with HpaII and TaqI produced profiles that enabled discrimination of type strains and identification of 7

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26

Cai, Hugh Y., Hazel Alexander, Susy Carman, Dara Lloyd, Gaylan Josephson, and M. Grant Maxie. "Restriction Fragment Length Polymorphism of Porcine Reproductive and Respiratory Syndrome Viruses Recovered from Ontario Farms, 1998–2000." Journal of Veterinary Diagnostic Investigation 14, no. 4 (2002): 343–47. http://dx.doi.org/10.1177/104063870201400415.

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From January 1998 to July 2000, 2,456 clinical samples, including lung, tonsil, lymph node, and serum, from 760 cases submitted to the Animal Health Laboratory, Ontario, Canada, were tested for porcine reproductive and respiratory syndrome viruses (PRRSV) using reverse transcriptase polymerase chain reaction (RT-PCR) and RT-PCR product restriction fragment length polymorphism (RFLP) analysis. A total of 516 samples from 284 cases were RT-PCR positive for the PRRSV open reading frame (ORF) 7 sequence. The RT-PCR RFLP typing assay was performed using 2 different sets of primers, amplifying 716 o

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27

Tarach, Piotr. "Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)." Acta Universitatis Lodziensis. Folia Biologica et Oecologica 17 (September 29, 2021): 48–53. http://dx.doi.org/10.18778/1730-2366.16.14.

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Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of dig

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28

Parkes, Richard, Teresa Lo, Quantine Wong, Judith L. Isaac-Renton, and Sean K. Byrne. "Comparison of a nested polymerase chain reaction restriction fragment length polymorphism method, the PATH antigen detection method, and microscopy for the detection and identification of malaria parasites." Canadian Journal of Microbiology 47, no. 10 (2001): 903–7. http://dx.doi.org/10.1139/w01-089.

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A nested polymerase chain reaction restriction fragment length polymorphism (PCRRFLP) method, the PATH antigen detection method, and light microscopy were compared for their capacity to detect and identify Plasmodium species. One hundred and thirty-six blood specimens obtained from patients suspected of having malaria were examined by each of the three methods. Forty-four specimens were positive for malaria using microscopy as the "gold standard". The sensitivity for nested PCR was 100%, and the specificity was 98%. For the detection of Plasmodium falciparum, the antigen detection method ha

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29

Marshall, S., C. G. Clark, G. Wang, M. Mulvey, M. T. Kelly, and W. M. Johnson. "Comparison of Molecular Methods for TypingVibrio parahaemolyticus." Journal of Clinical Microbiology 37, no. 8 (1999): 2473–78. http://dx.doi.org/10.1128/jcm.37.8.2473-2478.1999.

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An outbreak of Vibrio parahaemolyticus gastroenteritis on Canada’s west coast in 1997 emphasized the need to develop molecular methods for differentiation and typing of these organisms. Isolates were analyzed by enterobacterial repetitive intergenic consensus sequence (ERIC) PCR, detection of restriction fragment length polymorphisms (RFLP) in rRNA genes (ribotyping), pulsed-field gel electrophoresis (PFGE), and RFLP analysis of the genetic locus encoding the polar flagellum (Fla locus RFLP analysis). ERIC PCR and ribotyping were the most informative typing methods, especially when used togeth

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30

Garcia, Flávio C. Barbosa, Sandra Silva Rodrigues dos Santos, Maria Fernanda Chociay, Ângela C. Rapela Medeiros, and Ana Maria F. Roselino. "Métodos subsidiários para o diagnóstico da Leishmaniose tegumentar americana (LTA): comparação dos resultados do seqüenciamento de DNA e da PCR-RFLP para determinação da espécie de leishmania em amostras cutâneo-mucosas." Anais Brasileiros de Dermatologia 80, suppl 3 (2005): S339—S344. http://dx.doi.org/10.1590/s0365-05962005001000013.

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FUNDAMENTOS: Métodos moleculares têm-se mostrados mais eficazes para o diagnóstico da LTA. OBJETIVOS: Comparar os resultados da intradermorreação de Montenegro (IRM), presença de leishmania em biópsia (Bc), reação de imunofluorescência indireta (Rifi), seqüenciamento de DNA e PCR-RFLP (-restriction fragment lenght polymorphism) para o diagnóstico da LTA. MÉTODOS: Foram estudados 152 pacientes com LTA. Para a PCR em Bc, utilizaram-se primers específicos para seqüência de 120bp do kDNA do minicírculo comum a todas as espécies de leishmanias. O produto da PCR, utilizado para seqüenciamento e para

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Duttweiler, K. B., G. Y. Sun, J. C. Batzer, T. C. Harrington, and M. L. Gleason. "An RFLP-Based Technique for Identifying Fungi in the Sooty Blotch and Flyspeck Complex on Apple." Plant Disease 92, no. 5 (2008): 794–99. http://dx.doi.org/10.1094/pdis-92-5-0794.

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A restriction fragment length polymorphism (RFLP)-based technique was developed to identify members of the sooty blotch and flyspeck (SBFS) disease complex on apple because these fungi are difficult to identify using agar-plate isolation and morphological description. The method includes polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) using a fungal-specific forward primer (ITS1-F) and an SBFS-specific reverse primer (Myc1-R), followed by digestion of the PCR product by the HaeIII restriction enzyme. When applied to previous

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Haak, Wolfgang, Joachim Burger, and Kurt Werner Alt. "ABO genotyping by PCR-RFLP and cloning and sequencing." Anthropologischer Anzeiger 62, no. 4 (2004): 397–410. http://dx.doi.org/10.1127/anthranz/62/2004/397.

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33

Shanmugasundaram, Mahesh, Rajendran Chellaiah, and V. A Sajeev Kumar. "Detection of Meat Adulteration by PCR-RFLP: An Update." International Journal of Science and Research (IJSR) 11, no. 8 (2022): 1081–91. http://dx.doi.org/10.21275/sr22816155551.

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Cheng, Yu-Huei, Li-Yeh Chuang, and Cheng-Hong Yang. "Machine Learning Combined with Restriction Enzyme Mining Assists in the Design of Multi-Point Mutagenic Primers." Mathematics 10, no. 21 (2022): 4105. http://dx.doi.org/10.3390/math10214105.

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The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and high specificity; thus, many small and medium laboratories use it to perform all kinds of single nucleotide polymorphisms (SNPs) genotyping works, and as a molecular biotechnology for disease-related analysis. However, many single nucleotide polymorphisms lack available restriction enzymes to distinguish the specific genotypes on a target SNP, and that causes the PCR-RFLP assay which is unavailable to be called

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Kremer, K., D. van Soolingen, R. Frothingham, et al. "Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility." Journal of Clinical Microbiology 37, no. 8 (1999): 2607–18. http://dx.doi.org/10.1128/jcm.37.8.2607-2618.1999.

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In this study, the currently known typing methods forMycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typing methods and in seven PCR-based assays. In all methods, one or more repetitive DNA elements were targeted. The strain typing and the DNA fingerprint analysis were performed in the laboratory most experienced in the respective method. To examine intralaboratory reproducibility, b

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Tanahashi, Toshihito, Masakazu Kita, Tadashi Kodama, et al. "Comparison of PCR-Restriction Fragment Length Polymorphism Analysis and PCR-Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants." Journal of Clinical Microbiology 38, no. 1 (2000): 165–69. http://dx.doi.org/10.1128/jcm.38.1.165-169.2000.

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ABSTRACT A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the Helicobacter pylori genes is widely used to differentiate strains. However, with this typing method only a single base change at a specific restriction site can be detected. In addition, it is unclear whether the nucleotide base change recognized by RFLP is related to a substitution of encoded amino acid. To examine the validity of the PCR-RFLP method, 933-bp PCR products were obtained from 41 different clinical H. pylori isolates and were digested with Sau 3A restriction endonuclease. Furthermore, the nucle

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Karaselek, Mehmet Ali, Hasan Kapaklı, Şükrü Nail Güner, et al. "A family screening of CD19 gene mutation by PCR-RFLP." European Journal of Clinical and Experimental Medicine 20, no. 2 (2022): 141–45. http://dx.doi.org/10.15584/ejcem.2022.2.1.

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Introduction and aim. Mutation(s) in the gene encoding the CD19 molecule affect CD19 protein expression and primary immunodeficiency (PID) occurs. The PCR-RFLP method, which is faster and cheaper than other mutation detection methods, is rarely used in the diagnosis of PID. The study aimed to genetically identify CD19 deficiency, which is a PID, using the PCR-RFLP method. Material and methods. A total of 8 patients and two healthy controls were included in the study and the relevant region genotypes in the CD19 gene were determined by performing PCR-RFLP analysis. Results. The index case, newb

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Kamei, Kazumasa, Masahiro Asakura, Srinuan Somroop, et al. "A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis." Journal of Medical Microbiology 63, no. 5 (2014): 659–66. http://dx.doi.org/10.1099/jmm.0.071498-0.

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Although Campylobacter jejuni and Campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other Campylobacter species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin (cdt) gene-based PCR-RFLP assay for the detection and differentiation of C. jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis. Previously designed common primers, which can amplify the cdtB gene of C. jejuni, C. coli and C. fetus, were used for detecting seven Campylobacter species and differentiat

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Abdel-Rahman, S. M. "Evidences reveal that cattle and buffalo evolutionary derived from the same ancestor based on cytogenetic and molecular markers." Biotehnologija u stocarstvu 22, no. 3-4 (2006): 1–9. http://dx.doi.org/10.2298/bah0604001a.

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Muscle-DNA from cattle and buffalo was extracted to amplify the mitochondrial DNA segment (cytochrome b gene) and the gene encoding species-specific repeat (SSR) region. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and SSR techniques were used to identify of species origin. Restriction analysis of PCR-RFLP of the mitochondrial cytochrome b segment and SSR analysis showed no differences between cattle and buffalo. Where, the fragment length (bp) generated by AluI PCR-RFLP were 190, 169 and PCR amplification size of the gene encoding SSR region was 603 bp in both

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Peng, Stanford L., and Joseph Craft. "PCR-RFLP Genotyping of Murine MHC Haplotypes." BioTechniques 21, no. 3 (1996): 362–68. http://dx.doi.org/10.2144/96213bm03.

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Sheikina, Olga. "PCR-RFLP analysis of rbcL chloroplast gene." BIO Web of Conferences 43 (2022): 03007. http://dx.doi.org/10.1051/bioconf/20224303007.

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To identify intergeneric, interspecific, and intraspecific polymorphisms, PCR-RFLP studies of the chloroplast rbcL gene profiles were carried out using five restriction endonucleases (AluI, HpaII, BsuRI, BstHHI, BstMBI) in 14 species belonging to 6 different genera and 7 Thuja occidentalis cultivars. For all the genera studied (Pinus sp., Abies sp., Picea sp., Microbiota sp., Syringa sp., Thuja sp.), A genus-specific restriction profile characterized by a unique combination of restriction DNA fragments was revealed. The combination of enzymes used in the study did not reveal the interspecies a

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Rolshausen, P. E., F. Trouillas, and W. D. Gubler. "Identification of Eutypa lata by PCR-RFLP." Plant Disease 88, no. 9 (2004): 925–29. http://dx.doi.org/10.1094/pdis.2004.88.9.925.

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Eutypa lata is a vascular canker pathogen of woody plants commonly diagnosed by isolating the pathogen from infected tissue. Related fungi from the same family, the Diatrypaceae, also have been found in association with grapevine in Californian vineyards. An in situ polymerase chain reaction (PCR) method has been developed for detection of E. lata in infected wood tissue. However, our results indicate that this method also would amplify other Diatrypaceous fungi, which could potentially lead to an incorrect diagnosis. Therefore, we developed a PCR-restriction fragment length polymorphism (PCR-

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Wolf, Christian, Philipp Hübner, and Jürg Lüthy. "Differentiation of sturgeon species by PCR-RFLP." Food Research International 32, no. 10 (1999): 699–705. http://dx.doi.org/10.1016/s0963-9969(99)00150-7.

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Awaluddin, Awaluddin, Rizalinda Sjahrir, and Farida Ilyas. "PENGGUNAAN METODE PCR – RFLP (POLYMERASE CHAIN REACTION – RESTRICTION FRAGMENT LENGTH POLYMORFISM) DALAM MENDETEKSI JAMUR DERMATOFIT." JURNAL MEDIA KESEHATAN 14, no. 1 (2021): 96–102. http://dx.doi.org/10.33088/jmk.v14i1.615.

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ABSTRAK Dermatofitosis adalah salah satu jamur yang terdiri dari tiga genus : Epidermophyton, Trichophyton dan Microsporum. Penelitian ini bertujuan untuk mengidentifikasi jenis jamur dermatofit dengan metode PCR-RFLP. Penelitian ini merupakan penelitian observasional laboratorium dengan dengan menguji 23 sampel yang diperoleh dari beberapa klinik dan sekolah dasar di Makassar. Hasil penelitian menunjukkan bahwa semua sampel teridentifikasi yakni M. canis 26,1%, Trichophyton rubrum 13,1%,T. mentagrophytes 21,6%, T. tonsurans 8,7%, T. verrucosum 4,2% dan spesies unclassified 26,1%.

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Ozbes, G., Ertas HB, and A. Muzo. "Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey." Veterinární Medicína 48, No. 12 (2012): 359–62. http://dx.doi.org/10.17221/5790-vetmed.

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Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay. The assay amplifies a 743 bp fragment of the IBDV VP2 gene. The RFLP profiles of 40 of these positive samples were determined using the enzyme MboI. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity exists

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GHIMIRE, S. C., and J. R. EGERTON. "PCR–RFLP of outer membrane proteins gene of Dichelobacter nodosus: a new tool in the epidemiology of footrot." Epidemiology and Infection 122, no. 3 (1999): 521–28. http://dx.doi.org/10.1017/s0950268899002290.

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Currently only phenotypic epidemiological markers, serogrouping and virulence testing of Dichelobacter nodosus, are available for investigating footrot outbreaks in small ruminants. These methods have limitations in tracing the source of infection. In this study, a genotypic marker, PCR–RFLP of outer membrane protein gene, was used to characterize D. nodosus. The technique was evaluated in a controlled experiment involving two strains of bacteria. PCR–RFLP was found to be highly specific in differentiating isolates obtained from recipient animals infected with different strains. Subsequently,

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Omar, Nancy Younis, Hala Abdel Salam Ali, Reem Abdel Hameed Harfoush, and Engy Hamdy El Khayat. "Molecular Typing of Methicillin ResistantStaphylococcus aureusClinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms." International Journal of Microbiology 2014 (2014): 1–11. http://dx.doi.org/10.1155/2014/650328.

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Increased frequency of methicillin-resistantStaphylococcus aureus(MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) gene polymorphisms to verify their ability in assessing the relatedness of isolates. Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibio

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GILES, M., D. C. WARHURST, K. A. WEBSTER, D. M. WEST, and J. A. MARSHALL. "A multiplex allele specific polymerase chain reaction (MAS-PCR) on the dihydrofolate reductase gene for the detection of Cryptosporidium parvum genotypes 1 and 2." Parasitology 125, no. 1 (2002): 35–44. http://dx.doi.org/10.1017/s0031182002001786.

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A multiplex allele specific polymerase chain reaction (MAS-PCR) based on the Cryptosporidium parvum dihydrofolate reductase (dhfr) gene sequence differentiates genotype 1 (‘Human’) from 2 (‘Cattle’) in a 1-step reaction. The MAS-PCR was validated on a panel of 34 microscopically positive C. parvum faecal samples of human and animal origin in comparison with 2 published PCR-restriction fragment length polymorphism (RFLP) methods targeting dhfr and the oocyst wall protein (cowp) genes. A validation panel of 37 negative faecal samples of human and animal origin was also tested in comparison with

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Waleron, M., K. Waleron, and E. Łojkowska. "Genotypic characterisation of the Erwinia genus by PCR-RFLP analysis of rpoS gene." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 288–90. http://dx.doi.org/10.17221/10470-pps.

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Genotypic characterisation of the members of the genus Erwinia, based on the PCR-RFLP analysis of a fragment of the rpoS gene was done. PCR primers deduced from described rpoS gene sequences of E. carotovora allowed the amplification of about 880 bp DNA fragments from all tested Erwinia species. The rpoS fragments, amplified from 20 species of the studied Erwinia genus, were compared by RFLP analysis with 4 enzymes (AluI, Hin6I, HinfI, and Tru1I). Restriction analysis allowed drawing 63 common profiles of RFLP products for all tested Erwinia. From 1 to 3 specific RFLP profiles were identified

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Loubinoux, Julien, Alain Lozniewski, Christine Lion, Daniel Garin, Michèle Weber, and Alain E. Le Faou. "Value of Enterobacterial Repetitive Intergenic Consensus PCR for Study of Pasteurella multocidaStrains Isolated from Mouths of Dogs." Journal of Clinical Microbiology 37, no. 8 (1999): 2488–92. http://dx.doi.org/10.1128/jcm.37.8.2488-2492.1999.

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Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l’Armée de Terre, Suippes, France) were studied by use of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and restriction fragment length polymorphism (RFLP) techniques. Both techniques showed genomic heterogeneity of the strains studied. However, RFLP was more discriminatory than ERIC-PCR for differentiating P. multocida strains. All bu

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