Log in

Relevant bibliographies by topics / PCR ribotypes / Journal articles

To see the other types of publications on this topic, follow the link: PCR ribotypes.

Journal articles on the topic 'PCR ribotypes'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'PCR ribotypes.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Lee, Jong-Han, Yangsoon Lee, Kyungwon Lee, Thomas V. Riley, and Heejung Kim. "The changes of PCR ribotype and antimicrobial resistance of Clostridium difficile in a tertiary care hospital over 10 years." Journal of Medical Microbiology 63, no. 6 (2014): 819–23. http://dx.doi.org/10.1099/jmm.0.072082-0.

Full text

Abstract:

The aims of this study were to investigate any change in PCR ribotypes and to determine the antimicrobial resistance of common PCR ribotypes over a 10-year period in a tertiary care hospital. We conducted PCR ribotyping, antimicrobial susceptibility testing and DNA gyrase sequencing to identify changes in 1407 Clostridium difficile non-duplicated isolates obtained between 2000 and 2009. A total of 74 different ribotypes were found. The most prevalent ribotype was ribotype 001 (26.1 %). The prevalence of ribotype 017 was 17 % and that of ribotype 014/020 was 9.6 %. Ribotyping showed that the pr

APA, Harvard, Vancouver, ISO, and other styles

2

Tenover, Fred C., Isabella A. Tickler, and David H. Persing. "Antimicrobial-Resistant Strains of Clostridium difficile from North America." Antimicrobial Agents and Chemotherapy 56, no. 6 (2012): 2929–32. http://dx.doi.org/10.1128/aac.00220-12.

Full text

Abstract:

ABSTRACTA total of 316 toxigenicClostridium difficileclinical isolates of known PCR ribotypes from patients in North America were screened for resistance to clindamycin, metronidazole, moxifloxacin, and rifampin. Clindamycin resistance was observed among 16 different ribotypes, with ribotypes 017, 053, and 078 showing the highest proportions of resistance. All isolates were susceptible to metronidazole. Moxifloxacin resistance was present in >90% of PCR-ribotype 027 and 053 isolates but was less common among other ribotypes. Only 7.9% of theC. difficileisolates were resistant to rifampin. M

APA, Harvard, Vancouver, ISO, and other styles

3

Schneeberg, Alexander, Heinrich Neubauer, Gernot Schmoock, Ernst Grossmann, and Christian Seyboldt. "Presence of Clostridium difficile PCR ribotype clusters related to 033, 078 and 045 in diarrhoeic calves in Germany." Journal of Medical Microbiology 62, no. 8 (2013): 1190–98. http://dx.doi.org/10.1099/jmm.0.056473-0.

Full text

Abstract:

This study provides data on the distribution and relationship of C. difficile PCR ribotypes in diarrhoeic calves in Germany. C. difficile was isolated from 176 of 999 (17.6 %) faecal samples or swabs of diarrhoeic calves from 603 farms collected between January 2010 and August 2012 by eight federal laboratories of six states. Strains were assigned to 17 PCR ribotypes. PCR ribotypes 033 (57 %), 078 (17 %) and 045/FLI01 (closest match to 045 in the WEBRIBO database; 9 %) were found the most frequently. Nine per cent of all culture-positive tested animals shed more than one multiple locus variabl

APA, Harvard, Vancouver, ISO, and other styles

4

Indra, A., S. Huhulescu, M. Schneeweis, et al. "Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping." Journal of Medical Microbiology 57, no. 11 (2008): 1377–82. http://dx.doi.org/10.1099/jmm.0.47714-0.

Full text

Abstract:

We have developed a Clostridium difficile PCR ribotyping method based on capillary gel electrophoresis and have compared it with conventional PCR ribotyping. A total of 146 C. difficile isolates were studied: five isolates were reference strains (PCR ribotypes 001, 014, 017, 027 and 053); 141 were clinical isolates comprising 39 Austrian PCR ribotypes collected in the period 2006–2007 at 25 Austrian healthcare facilities. Capillary gel electrophoresis yielded up to 11 fragments per isolate and 47 ribotype patterns. All but one of the five PCR ribotypes of reference strains were clearly reflect

APA, Harvard, Vancouver, ISO, and other styles

5

Knetsch, Cornelis W., Marjolein P. M. Hensgens, Céline Harmanus, et al. "Genetic markers for Clostridium difficile lineages linked to hypervirulence." Microbiology 157, no. 11 (2011): 3113–23. http://dx.doi.org/10.1099/mic.0.051953-0.

Full text

Abstract:

Rapid identification of hypervirulent Clostridium difficile strains is essential for preventing their spread. Recent completion of several full-length C. difficile genomes provided an excellent opportunity to identify potentially unique genes that characterize hypervirulent strains. Based on sequence comparisons between C. difficile strains we describe two gene insertions into the genome of hypervirulent PCR ribotypes 078 and 027. Analysis of these regions, of 1.7 and 4.2 kb, respectively, revealed that they contain several interesting ORFs. The 078 region is inserted intergenically and introd

APA, Harvard, Vancouver, ISO, and other styles

6

JAGLIC Z, Z., Z. KUCEROVA, K. NEDBALCOVA, I. PAVLIK, P. ALEXA, and M. BARTOS. "Characterisation and comparison of Pasteurella multocidaisolated from different species in theCzechRepublic: capsular PCR typing, ribotyping and dermonecrotoxin production." Veterinární Medicína 50, No. 8 (2012): 345–54. http://dx.doi.org/10.17221/5633-vetmed.

Full text

Abstract:

The aim of this study was to characterise and compare Pasteurella multocida isolates originating from pigs (n&nbsp;=&nbsp;43), calves (n&nbsp;=&nbsp;31), rabbits (n&nbsp;=&nbsp;27), and to a lesser extent from other hosts (n&nbsp;=&nbsp;6). A total of 107 P. multocida isolates were obtained from various locations in the Czech Republic. They were analysed by capsular PCR typing and ribotyping, and tested for the production of dermonecrotoxin. Most frequently, serogroup A isolates (n&nbsp;=&nbsp;74) were found, followed by serogroup D (n&nbsp;=&nbs

APA, Harvard, Vancouver, ISO, and other styles

7

Zidaric, Valerija, Bart Pardon, Tiago dos Vultos, et al. "Different Antibiotic Resistance and Sporulation Properties within Multiclonal Clostridium difficile PCR Ribotypes 078, 126, and 033 in a Single Calf Farm." Applied and Environmental Microbiology 78, no. 24 (2012): 8515–22. http://dx.doi.org/10.1128/aem.02185-12.

Full text

Abstract:

ABSTRACTClostridium difficilestrains were sampled periodically from 50 animals at a single veal calf farm over a period of 6 months. At arrival, 10% of animals wereC. difficilepositive, and the peak incidence was determined to occur at the age of 18 days (16%). The prevalence then decreased, and at slaughter,C. difficilecould not be isolated. Six different PCR ribotypes were detected, and strains within a single PCR ribotype could be differentiated further by pulsed-field gel electrophoresis (PFGE). The PCR ribotype diversity was high up to the animal age of 18 days, but at later sampling poin

APA, Harvard, Vancouver, ISO, and other styles

8

Schneeberg, Alexander, Ralf Ehricht, Peter Slickers, et al. "DNA Microarray-Based PCR Ribotyping of Clostridium difficile." Journal of Clinical Microbiology 53, no. 2 (2014): 433–42. http://dx.doi.org/10.1128/jcm.02524-14.

Full text

Abstract:

This study presents a DNA microarray-based assay for fast and simple PCR ribotyping ofClostridium difficilestrains. Hybridization probes were designed to query the modularly structured intergenic spacer region (ISR), which is also the template for conventional and PCR ribotyping with subsequent capillary gel electrophoresis (seq-PCR) ribotyping. The probes were derived from sequences available in GenBank as well as from theoretical ISR module combinations. A database of reference hybridization patterns was set up from a collection of 142 well-characterizedC. difficileisolates representing 48 s

APA, Harvard, Vancouver, ISO, and other styles

9

Tickler, Isabella A., Richard V. Goering, Joseph D. Whitmore, Ashley N. W. Lynn, David H. Persing, and Fred C. Tenover. "Strain Types and Antimicrobial Resistance Patterns of Clostridium difficile Isolates from the United States, 2011 to 2013." Antimicrobial Agents and Chemotherapy 58, no. 7 (2014): 4214–18. http://dx.doi.org/10.1128/aac.02775-13.

Full text

Abstract:

ABSTRACTWe determined the PCR ribotypes and antimicrobial susceptibility patterns of 508 toxigenicClostridium difficileisolates collected between 2011 and 2013 from 32 U.S. hospitals. Of the 29 PCR ribotypes identified, the 027 strain type was the most common (28.1%), although the rates varied by geographic region. Ribotype 014/020 isolates appear to be emerging. Clindamycin and moxifloxacin resistances (36.8% and 35.8%, respectively) were the most frequent resistance phenotypes observed. Reduced susceptibility to vancomycin was observed in 39.1% of 027 isolates.

APA, Harvard, Vancouver, ISO, and other styles

10

Romano, Vincenza, Vincenzo Pasquale, Karel Krovacek, Federica Mauri, Antonella Demarta, and Stefano Dumontet. "Toxigenic Clostridium difficile PCR Ribotypes from Wastewater Treatment Plants in Southern Switzerland." Applied and Environmental Microbiology 78, no. 18 (2012): 6643–46. http://dx.doi.org/10.1128/aem.01379-12.

Full text

Abstract:

ABSTRACTThe occurrence ofClostridium difficilein nine wastewater treatment plants in the Ticino Canton (southern Switzerland) was investigated. The samples were collected from raw sewage influents and from treated effluents. Forty-seven out of 55 characterizedC. difficilestrains belonged to 13 different reference PCR ribotypes (009, 010, 014, 015, 039, 052, 053, 066, 070, 078, 101, 106, and 117), whereas 8 strains did not match any of those available in our libraries. The most frequently isolated ribotype (40%) was 078, isolated from six wastewater treatment plants, whereas ribotype 066, a tox

APA, Harvard, Vancouver, ISO, and other styles

11

Ní Eidhin, Déirdre, Anthony W. Ryan, Rachael M. Doyle, J. Bernard Walsh, and Dermot Kelleher. "Sequence and phylogenetic analysis of the gene for surface layer protein, slpA, from 14 PCR ribotypes of Clostridium difficile." Journal of Medical Microbiology 55, no. 1 (2006): 69–83. http://dx.doi.org/10.1099/jmm.0.46204-0.

Full text

Abstract:

Clostridium difficile is the commonest cause of antibiotic-associated diarrhoea, with the hospitalized elderly being at particular risk. The organism makes a crystalline surface protein layer (S-layer), encoded by the slpA gene, the product of which is cleaved to give two mature peptides which associate to form the layer. The larger peptide (high molecular weight; HMW), derived from the C-terminal portion of the precursor, is relatively conserved, whereas the smaller peptide (low molecular weight; LMW), derived from the N-terminal portion of the precursor, is a dominant antigen which substanti

APA, Harvard, Vancouver, ISO, and other styles

12

Rafila, Alexandru, Alexander Indra, Gabriel Adrian Popescu, et al. "Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania." Journal of Infection in Developing Countries 8, no. 06 (2014): 694–98. http://dx.doi.org/10.3855/jidc.4435.

Full text

Abstract:

Introduction: Little is known about prevailing ribotypes of Clostridium difficile infection in Romania where CDI is not a mandatory notifiable disease. Methodology: We studied 64 non-duplicate C. difficile isolates from patients hospitalised at the National Institute of Infectious Diseases, Bucharest, Romania between March 2011 and March 2012. Results: Sixty-three of the 64 C. difficile isolates produced toxins A and B whereas 44 (69%) isolates produced a binary toxin. Ribotype 027 accounted for 43 (68%) of the 63 toxigenic strains. The remaining 20 isolates belonged to ribotypes 018 (n = 9),

APA, Harvard, Vancouver, ISO, and other styles

13

Snydman, David R., Laura A. McDermott, Stephen G. Jenkins, et al. "Epidemiologic Trends in Clostridium difficile Isolate Ribotypes in United States from 2010 to 2014." Open Forum Infectious Diseases 4, suppl_1 (2017): S391. http://dx.doi.org/10.1093/ofid/ofx163.973.

Full text

Abstract:

Abstract Background Trends in the distribution of ribotypes for C. difficile associated diarrheal isolates obtained over time in the United States are lacking. As part of surveillance program for C. difficile susceptibility, we analyzed stool isolates for ribotype distribution from a phase 2 trial of surotomycin (2010–2011) (North America sites) as well as a national surveillance study from 2011–2014. Isolates for the surveillance study were referred from 6 geographically distinct medical centers. Methods C. difficile isolates or C. difficile toxin + stools from patients with C. difficile asso

APA, Harvard, Vancouver, ISO, and other styles

14

Aitken, Samuel L., M. Jahangir Alam, Mohammed Khaleduzzuman, et al. "In the Endemic Setting, Clostridium difficile Ribotype 027 Is Virulent But Not Hypervirulent." Infection Control & Hospital Epidemiology 36, no. 11 (2015): 1318–23. http://dx.doi.org/10.1017/ice.2015.187.

Full text

Abstract:

BACKGROUNDConflicting reports have been published on the association between Clostridium difficile ribotypes and severe disease outcomes in patients with C. difficile infection (CDI); several so-called hypervirulent ribotypes have been described. We performed a multicenter study to assess severe disease presentation and severe outcomes among CDI patients infected with different ribotypes.METHODSStool samples that tested positive for C. difficile toxin were collected and cultured from patients who presented to any of 7 different hospitals in Houston, Texas (2011–2013). C. difficile was characte

APA, Harvard, Vancouver, ISO, and other styles

15

Hoo Jeon, Cheon, and Yu Mi Wi. "2372. PCR Ribotype and Antimicrobial Susceptibility of Clostridioides (Formerly Clostridium) difficile in Korea." Open Forum Infectious Diseases 6, Supplement_2 (2019): S818. http://dx.doi.org/10.1093/ofid/ofz360.2050.

Full text

Abstract:

Abstract Background Clostridioides difficile infection is a leading cause of healthcare-associated diarrhea. The epidemiology and characteristics of C. difficile vary geographically. We performed toxin enzyme immunoassay (EIA), toxigenic gene analysis, antimicrobial susceptibility tests (AST), and PCR ribotyping to elucidate the characteristics of C. difficile in Korea. Methods Between July 2017 and June 2018, C. difficile was prospectively isolated in 128 specimens from the culture of 1,182 unduplicated specimens. Seventy-five stool specimens with a positive toxin EIA between July 2016 and Ju

APA, Harvard, Vancouver, ISO, and other styles

16

Vohra, Prerna, and Ian R. Poxton. "Comparison of toxin and spore production in clinically relevant strains of Clostridium difficile." Microbiology 157, no. 5 (2011): 1343–53. http://dx.doi.org/10.1099/mic.0.046243-0.

Full text

Abstract:

Clostridium difficile is a major cause of nosocomial diarrhoea. The toxins that it produces (TcdA and TcdB) are responsible for the characteristic pathology of C. difficile infection (CDI), while its spores persist in the environment, causing its widespread transmission. Many different strains of C. difficile exist worldwide and the epidemiology of the strains is ever-changing: in Scotland, PCR ribotype 012 was once prevalent, but currently ribotypes 106, 001 and 027 are endemic. This study aimed to identify the differences among these ribotypes with respect to their growth, and toxin and spor

APA, Harvard, Vancouver, ISO, and other styles

17

Rodriguez-Palacios, Alexander, Sanja Ilic, and Jeffrey T. LeJeune. "Subboiling Moist Heat Favors the Selection of Enteric PathogenClostridium difficilePCR Ribotype 078 Spores in Food." Canadian Journal of Infectious Diseases and Medical Microbiology 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/1462405.

Full text

Abstract:

Emerging enteric pathogens could have not only more antibiotic resistance or virulence traits; they could also have increased resistance to heat. We quantified the effects of minimum recommended cooking and higher temperatures, individually on a collection ofC. difficileisolates and on the survival probability of a mixture of emergingC. difficilestrains. While minimum recommended cooking time/temperature combinations (63–71°C) allowed concurrently tested strains to survive, higher subboiling temperatures reproducibly favored the selection of newly emergingC. difficilePCR ribotype 078. Survival

APA, Harvard, Vancouver, ISO, and other styles

18

Taori, Surabhi K., Val Hall, and Ian R. Poxton. "Changes in antibiotic susceptibility and ribotypes in Clostridium difficile isolates from southern Scotland, 1979–2004." Journal of Medical Microbiology 59, no. 3 (2010): 338–44. http://dx.doi.org/10.1099/jmm.0.014829-0.

Full text

Abstract:

An increase in the incidence of clinical cases of Clostridium difficile infection has been reported in recent years, but few studies have examined changes in molecular epidemiology and antibiotic resistance over a long period of time. A collection of 179 isolates of C. difficile obtained from symptomatic adult patients in southern Scotland between 1979 and 2004 was used to determine changes in the prevalence of epidemiological types and antibiotic susceptibilities to common antibiotics. PCR ribotyping and MIC determination were performed on all isolates. A total of 56 different ribotypes were

APA, Harvard, Vancouver, ISO, and other styles

19

Pirš, Tina, Jana Avberšek, Irena Zdovc, et al. "Antimicrobial susceptibility of animal and human isolates of Clostridium difficile by broth microdilution." Journal of Medical Microbiology 62, no. 9 (2013): 1478–85. http://dx.doi.org/10.1099/jmm.0.058875-0.

Full text

Abstract:

A total of 188 human (n = 92) and animal (n = 96) isolates of Clostridium difficile of different PCR ribotypes were screened for susceptibility to 30 antimicrobials using broth microdilution. When comparing the prevalence of antimicrobial resistance, the isolates of animal origin were significantly more often resistant to oxacillin, gentamicin and trimethoprim/sulfamethoxazole (P<0.01). The most significant difference between the animal and human populations (P = 0.0006) was found in the level of imipenem resistance, with a prevalence of 53.3 % in isolates of human origin and 28.1 % in isol

APA, Harvard, Vancouver, ISO, and other styles

20

Dobreva, Elina G., Ivan N. Ivanov, Rossitza S. Vathcheva-Dobrevska, et al. "Advances in molecular surveillance of Clostridium difficile in Bulgaria." Journal of Medical Microbiology 62, no. 9 (2013): 1428–34. http://dx.doi.org/10.1099/jmm.0.058149-0.

Full text

Abstract:

The increasing incidence of Clostridium difficile infection (CDI) in Bulgaria has indicated the need to implement better surveillance approaches. The aim of the present work was to improve the current surveillance of CDI in Bulgaria by introducing innovative methods for identification and typing. One hundred and twenty stool samples obtained from 108 patients were studied over 4 years from which 32 C. difficile isolates were obtained. An innovative duplex EvaGreen real-time PCR assay based on simultaneous detection of the gluD and tcdB genes was developed for rapid C. difficile identification.

APA, Harvard, Vancouver, ISO, and other styles

21

Wood, Jacqueline, Karen P. Scott, Gorazd Avguštin, C. James Newbold, and Harry J. Flint. "Estimation of the Relative Abundance of DifferentBacteroides and Prevotella Ribotypes in Gut Samples by Restriction Enzyme Profiling of PCR-Amplified 16S rRNA Gene Sequences." Applied and Environmental Microbiology 64, no. 10 (1998): 3683–89. http://dx.doi.org/10.1128/aem.64.10.3683-3689.1998.

Full text

Abstract:

ABSTRACT We describe an approach for determining the genetic composition ofBacteroides and Prevotellapopulations in gut contents based on selective amplification of 16S rRNA gene sequences (rDNA) followed by cleavage of the amplified material with restriction enzymes. The relative contributions of different ribotypes to total Bacteroides andPrevotella 16S rDNA are estimated after end labelling of one of the PCR primers, and the contribution ofBacteroides and Prevotellasequences to total eubacterial 16S rDNA is estimated by measuring the binding of oligonucleotide probes to amplified DNA.Bacter

APA, Harvard, Vancouver, ISO, and other styles

22

Munoz-Price, L. Silvia, Nathan A. Ledeboer, Isabella A. Tickler, et al. "2399. Ribotype Diversity of Clostridioides difficile strains obtained during screening tests." Open Forum Infectious Diseases 6, Supplement_2 (2019): S828—S829. http://dx.doi.org/10.1093/ofid/ofz360.2077.

Full text

Abstract:

Abstract Background Clostridioides difficile is an organism acquired not only in healthcare settings but also in community settings. For the past several years our hospital has performed screening tests to detect asymptomatic carriage of C. difficile. We now aim to better understand the ribotypes and degree of diversity among these C. difficile strains obtained in a systematic screening. Methods This study was performed at a 600 bed teaching affiliated hospital in Milwaukee, WI, where surveillance testing is performed in selected units upon admission and weekly thereafter using nucleic acid am

APA, Harvard, Vancouver, ISO, and other styles

23

Calderaro, Adriana, Mirko Buttrini, Monica Martinelli, et al. "Rapid Classification of Clostridioides difficile Strains Using MALDI-TOF MS Peak-Based Assay in Comparison with PCR-Ribotyping." Microorganisms 9, no. 3 (2021): 661. http://dx.doi.org/10.3390/microorganisms9030661.

Full text

Abstract:

Typing methods are needed for epidemiological tracking of new emerging and hypervirulent strains because of the growing incidence, severity and mortality of Clostridioides difficile infections (CDI). The aim of this study was the evaluation of a typing Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS (T-MALDI)) method for the rapid classification of the circulating C. difficile strains in comparison with polymerase chain reaction (PCR)-ribotyping results. Among 95 C. difficile strains, 10 ribotypes (PR1–PR10) were identified by PCR-ribotyping. In particular,

APA, Harvard, Vancouver, ISO, and other styles

24

Biazzo, Manuele, Rossella Cioncada, Luigi Fiaschi, et al. "Diversity of cwp loci in clinical isolates of Clostridium difficile." Journal of Medical Microbiology 62, no. 9 (2013): 1444–52. http://dx.doi.org/10.1099/jmm.0.058719-0.

Full text

Abstract:

An increased incidence of Clostridium difficile infection (CDI) is associated with the emergence of epidemic strains characterized by high genetic diversity. Among the factors that may have a role in CDI is a family of 29 paralogues, the cell-wall proteins (CWPs), which compose the outer layer of the bacterial cell and are likely to be involved in colonization. Previous studies have shown that 12 of the 29 cwp genes are clustered in the same region, named after slpA (cwp1), the slpA locus, whereas the remaining 17 paralogues are distributed throughout the genome. The variability of 14 of these

APA, Harvard, Vancouver, ISO, and other styles

25

Rizzardi, Kristina, Thomas Åkerlund, Torbjörn Norén, and Andreas Matussek. "Impact of ribotype on Clostridioides difficile diagnostics." European Journal of Clinical Microbiology & Infectious Diseases 39, no. 5 (2019): 847–53. http://dx.doi.org/10.1007/s10096-019-03772-z.

Full text

Abstract:

AbstractThis study investigates the performance of diagnostic methods for detection of Clostridioides difficile infection in Sweden, including impact of PCR ribotype on diagnostic performance. Between 2011 and 2016, a total of 17,878 stool samples from 26 laboratories were tested by either well-type enzyme immunoassays (EIAs), membrane bound EIAs, cell cytotoxicity neutralization assay (CTA), or nucleic acid amplification tests (NAATs) and subsequently cultured for C. difficile. Roughly half of the samples (9454/17878) were subjected to diagnostic testing both on the fecal sample and on the 13

APA, Harvard, Vancouver, ISO, and other styles

26

Mihajlov, Kiril, Aneta Andreska, Nadica Ristovska, Tatjana Grdanoska, and Elena Trajkovska-Dokic. "Distribution of Clostridium Difficile Ribotypes in Macedonian Patients and their Antimicrobial Susceptibility." Open Access Macedonian Journal of Medical Sciences 7, no. 12 (2019): 1896–99. http://dx.doi.org/10.3889/oamjms.2019.482.

Full text

Abstract:

BACKGROUND: Clostridium difficile is a major nosocomial pathogen. In Europe, this bacterium is mostly characterised by PCR ribotyping. Most of the Clostridium difficile infections (CDI) are treated with vancomycin or metronidazole, although prolonged antibiotic use is considered as one of the main risk factors for CDI.AIM: This study aimed to detect the presence of various C. difficile ribotypes in hospitalised patients and to investigate their toxigenicity and antibiotic susceptibility.MATERIAL AND METHODS: All stool samples obtained from each patient were inoculated on Columbia blood agar an

APA, Harvard, Vancouver, ISO, and other styles

27

Pituch, Hanna, Jon S. Brazier, Piotr Obuch-Woszczatyński, Dorota Wultańska, Felicja Meisel-Mikołajczyk, and Mirosław Łuczak. "Prevalence and association of PCR ribotypes of Clostridium difficile isolated from symptomatic patients from Warsaw with macrolide-lincosamide-streptogramin B (MLSB) type resistance." Journal of Medical Microbiology 55, no. 2 (2006): 207–13. http://dx.doi.org/10.1099/jmm.0.46213-0.

Full text

Abstract:

Isolates (79 in total) of Clostridium difficile obtained over a 2 year period from 785 patients suspected of having C. difficile-associated diarrhoea (CDAD) and being hospitalized in the University Hospital in Warsaw were characterized by toxigenicity profile and PCR ribotyping. Furthermore, their susceptibility to clindamycin and erythromycin was determined. Among the 79 C. difficile isolates, 35 were classified as A+B+, 1 as A+B+CDT+, 36 as A−B+ and 7 as A−B−. A total of 21 different PCR ribotypes was detected. Two main A+B+ strains circulated in our hospital: ribotype 014 and ribotype 046.

APA, Harvard, Vancouver, ISO, and other styles

28

D'AMICO, DENNIS J., and CATHERINE W. DONNELLY. "Enhanced Detection of Listeria spp. in Farmstead Cheese Processing Environments through Dual Primary Enrichment, PCR, and Molecular Subtyping." Journal of Food Protection 71, no. 11 (2008): 2239–48. http://dx.doi.org/10.4315/0362-028x-71.11.2239.

Full text

Abstract:

The incidence and ecology of Listeria spp. in farmstead cheese processing environments were assessed through environmental sampling conducted in nine different plants over a 10-week period. Environmental samples (n = 705) were examined for the presence of Listeria spp. by using three detection/isolation protocols. The use of dual enrichment methods, which allowed for the recovery of injured Listeria spp. (mUSDA), identified more Listeria species–positive samples with higher sensitivity than the standard USDA method. The addition of PCR to the mUSDA method identified the most Listeria monocytog

APA, Harvard, Vancouver, ISO, and other styles

29

LANDMAN, D., M. BUTNARIU, S. BRATU, and J. QUALE. "Genetic relatedness of multidrug-resistantAcinetobacter baumanniiendemic to New York City." Epidemiology and Infection 137, no. 2 (2008): 174–80. http://dx.doi.org/10.1017/s0950268808000824.

Full text

Abstract:

SUMMARYMultidrug-resistant isolates ofAcinetobacter baumanniifrom New York City generally belong to one of three ribotypes. To assess the accuracy of ribotyping, the relatedness of representative isolates was further assessed by rep-PCR, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of five genes potentially associated with antimicrobial resistance (ampC,ompA,adeB,adeR, andabeM). The isolates fell into several major groups. The first group shared the same ribotype and had common mutations affecting OmpA, AdeR, and AbeM, but consisted of two subtypes with distinctive rep-PCR and P

APA, Harvard, Vancouver, ISO, and other styles

30

Saxton, Katie, Simon D. Baines, Jane Freeman, Rachael O'Connor, and Mark H. Wilcox. "Effects of Exposure of Clostridium difficile PCR Ribotypes 027 and 001 to Fluoroquinolones in a Human Gut Model." Antimicrobial Agents and Chemotherapy 53, no. 2 (2008): 412–20. http://dx.doi.org/10.1128/aac.00306-08.

Full text

Abstract:

ABSTRACT The incidence of Clostridium difficile infection is increasing, with reports implicating fluoroquinolone use. A three-stage chemostat gut model was used to study the effects of three fluoroquinolones (ciprofloxacin, levofloxacin, and moxifloxacin) on the gut microbiota and two epidemic C. difficile strains, strains of PCR ribotypes 027 and 001, in separate experiments. C. difficile total viable counts, spore counts, and cytotoxin titers were determined. The emergence of C. difficile isolates with reduced antibiotic susceptibility was monitored with fluoroquinolone-containing medium, a

APA, Harvard, Vancouver, ISO, and other styles

31

Sulakvelidze, Alexander, Merab Kekelidze, Tsaro Gomelauri, et al. "Diphtheria in the Republic of Georgia: Use of Molecular Typing Techniques for Characterization of Corynebacterium diphtheriae Strains." Journal of Clinical Microbiology 37, no. 10 (1999): 3265–70. http://dx.doi.org/10.1128/jcm.37.10.3265-3270.1999.

Full text

Abstract:

Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-GeorgianC. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the Elek assay and PCR, (iii) the randomly amplified polymorphic DNA (RAPD) technique, and (iv) pulsed-field gel electrophoresis (PFGE). Fifteen selected strains were ribotyped. Six RAPD types and 15 PFGE patterns were identified among all strains examined, and 12 ribotypes were found a

APA, Harvard, Vancouver, ISO, and other styles

32

Djebbar, Abla, Mohammed Sebaihia, Ed Kuijper, et al. "First molecular characterisation and PCR ribotyping of Clostridium difficile strains isolated in two Algerian Hospitals." Journal of Infection in Developing Countries 12, no. 01 (2018): 015–21. http://dx.doi.org/10.3855/jidc.9580.

Full text

Abstract:

Introduction: Clostridium difficile is the major etiological agent of nosocomial antibiotics associated diarrhoea. C. difficile infection is associated with considerable morbidity and mortality among hospitalized patients worldwide. Despite its known importance, there is no study on this important pathogen in Algeria. Methodology: In this prospective study, undertaken between 2013 and 2015, faecal specimens were collected from 159 hospitalized patients with antibiotic-associated diarrhoea in two tertiary health care hospitals in Chlef, Algeria. Faecal samples were cultured on CLO plates

APA, Harvard, Vancouver, ISO, and other styles

33

Bingol, Enver Baris, Hamparsun Hampikyan, Karlo Muratoglu, Esra Akkaya, Omer Cetin, and Hilal Colak. "Characterisation and antibiotic susceptibility profile of Clostridioides (Clostridium) difficile isolated from chicken carcasses." Journal of Veterinary Research 64, no. 3 (2020): 407–12. http://dx.doi.org/10.2478/jvetres-2020-0052.

Full text

Abstract:

AbstractIntroductionClostridioides (Clostridium) difficile is a Gram+, anaerobic, spore-forming, rod-shaped bacterium that can produce toxins, and it is mainly because its virulence is attributed. The objective of this study was to evaluate the presence of C. difficile and hyper virulent ribotypes in chicken carcasses and the antibiotic susceptibility of isolated strains.Material and MethodsC. difficile was isolated from chicken carcasses by microbiological methods, its ribotypes were identified by means of PCR, the toxin production ability was defined by ELISA, and the susceptibility of the i

APA, Harvard, Vancouver, ISO, and other styles

34

Guevara, Eduardo Yepez, Harika Yalamanchili, Andrew Chao, et al. "Ribotypes Matter, Significance of Clostridium difficile Ribotypes in Cancer Patients with Diarrhea." Open Forum Infectious Diseases 4, suppl_1 (2017): S386—S387. http://dx.doi.org/10.1093/ofid/ofx163.961.

Full text

Abstract:

Abstract Background Cancer patients are at increased risk for Clostridium difficile infection (CDI) due to frequent health care contact, chemotherapy, use of antibiotics, and immunosuppression. Distinct ribotypes are associated with CDI adverse outcomes. Ribotypes 14-020 are the predominant ribotypes in many hospitals. We examined the contribution of C. difficile ribotypes to CDI severity, response to therapy and outcomes in this population. Methods Demographic and clinical data were collected from 90 cancer patients with a first episode or first recurrence of CDI identified by two-step PCR fo

APA, Harvard, Vancouver, ISO, and other styles

35

Gonzales-Luna, Anne J., Travis J. Carlson, Kierra M. Dotson, et al. "PCR ribotypes of Clostridioides difficile across Texas from 2011 to 2018 including emergence of ribotype 255." Emerging Microbes & Infections 9, no. 1 (2020): 341–47. http://dx.doi.org/10.1080/22221751.2020.1721335.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Almutairi, Masaad, Kevin W. Garey, Faris S. Alnezary, et al. "784. A Novel Method to Assess Virulence of Clostridioides difficile: Focus on C. difficile Ribotype 106." Open Forum Infectious Diseases 7, Supplement_1 (2020): S436—S437. http://dx.doi.org/10.1093/ofid/ofaa439.974.

Full text

Abstract:

Abstract Background Clostridioides difficile ribotype (RT) 106 has emerged as one of the most commonly isolated strains in the USA and worldwide. However, studies investigating clinical outcomes associated with this strain are lacking. The purpose of this study was to compare disease severity, clinical cure, and recurrence rates associated with CDI caused by RT106 vs two other comparator strains. Methods This multicenter study (20 hospitals) assessed hospitalized patients infected with C. difficile RT106 compared to patients infected with a known hypervirulent strain (RT027) and a strain assoc

APA, Harvard, Vancouver, ISO, and other styles

37

Indra, A., D. Schmid, S. Huhulescu, et al. "Characterization of clinical Clostridium difficile isolates by PCR ribotyping and detection of toxin genes in Austria, 2006–2007." Journal of Medical Microbiology 57, no. 6 (2008): 702–8. http://dx.doi.org/10.1099/jmm.0.47476-0.

Full text

Abstract:

In order to assess the lethality of Clostridium difficile-associated disease (CDAD) and the PCR ribotypes prevalent in Austria, the Austrian Agency for Health and Food Safety requested isolates of C. difficile from patients in a structured but arbitrary sampling scheme. In the allocated period from February 2006 to January 2007, local hospital laboratories within each of the nine provinces were asked to submit C. difficile isolates from at least ten cases of CDAD. Confirmation of species identification, toxin detection, susceptibility testing against four antimicrobial agents and typing using

APA, Harvard, Vancouver, ISO, and other styles

38

Novakova, Elena, Zuzana Stofkova, Vladimira Sadlonova, and Lukas Hleba. "Diagnostic Methods of Clostridioides difficile Infection and Clostridioides difficile Ribotypes in Studied Sample." Antibiotics 10, no. 9 (2021): 1035. http://dx.doi.org/10.3390/antibiotics10091035.

Full text

Abstract:

Background: Clostridioides (Clostridium) difficile is the most common nosocomial pathogen and antibiotic-related diarrhea in health-care facilities. Over the last few years, there was an increase in the incidence rate of C. difficile infection cases in Slovakia. In this study, the phenotypic (toxigenicity, antimicrobial susceptibility) and genotypic (PCR ribotypes, genes for binary toxins) patterns of C. difficile isolates from patients with CDI were analyzed, from July to August 2016, taken from hospitals in the Horne Povazie region of northern Slovakia. The aim of the study was also to ident

APA, Harvard, Vancouver, ISO, and other styles

39

Behroozian, Adam A., Jeffrey P. Chludzinski, Eugene S. Lo, et al. "Detection of Mixed Populations ofClostridium difficilefrom Symptomatic Patients Using Capillary-Based Polymerase Chain Reaction Ribotyping." Infection Control & Hospital Epidemiology 34, no. 9 (2013): 961–66. http://dx.doi.org/10.1086/671728.

Full text

Abstract:

Objective.To investigate the simultaneous occurrence of more than 1Clostridium difficileribotype in patients' stool samples at the time of diagnostic testing.Methods.Stool samples submitted for diagnostic testing for the presence of toxigenicC. difficilewere obtained for 102 unique patients. A total of 95 single colonies ofC. difficileper stool sample were isolated on selective media, subcultured alongside negative (uninoculated) controls, and polymerase chain reaction (PCR) ribotyped using capillary gel electrophoresis.Results.Capillary-based PCR ribotyping was successful for 9,335C. difficil

APA, Harvard, Vancouver, ISO, and other styles

40

Cox, B. A., H. Luo, and R. A. C. Jones. "Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity." Plant Disease 98, no. 11 (2014): 1567–75. http://dx.doi.org/10.1094/pdis-02-14-0128-re.

Full text

Abstract:

Polymyxa graminis is an obligate parasite of roots and an important vector of viruses that damage cereal crops in different parts of the world. In 2011 and 2012, P. graminis was identified infecting 11 wheat root samples from three widely dispersed locations in southwest Australia. Its presence was detected by polymerase chain reaction (PCR) and confirmed by DNA sequencing of the transcribed regions of its ribosomal RNA genes (rDNA) and observing sporosori of characteristic morphology and size in stained wheat roots. Also, when soil samples were collected from two locations where P. graminis w

APA, Harvard, Vancouver, ISO, and other styles

41

Moura, I., P. Spigaglia, F. Barbanti, and P. Mastrantonio. "Analysis of metronidazole susceptibility in different Clostridium difficile PCR ribotypes." Journal of Antimicrobial Chemotherapy 68, no. 2 (2012): 362–65. http://dx.doi.org/10.1093/jac/dks420.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Terhes, Gabriella, Jon S. Brazier, Edit Urbán, József Sóki, and Elisabeth Nagy. "Distribution of Clostridium difficile PCR ribotypes in regions of Hungary." Journal of Medical Microbiology 55, no. 3 (2006): 279–82. http://dx.doi.org/10.1099/jmm.0.46141-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Begum, Khurshida, M. Jahangir Alam, Jacob McPherson, et al. "2410. Molecular Characteristics of Environmental Clostridioides difficile From a Large Texas Hospital." Open Forum Infectious Diseases 6, Supplement_2 (2019): S832. http://dx.doi.org/10.1093/ofid/ofz360.2088.

Full text

Abstract:

Abstract Background Clostridioides difficile is an anaerobic spore-forming, toxin-producing Gram-positive bacillus listed by the CDC as an “urgent threat” pathogen. Epidemiologic studies using whole-genome sequencing (WGS) have found that genetically distinct lineages infections occur in hospitalized patients, in addition to the fact that C. difficile spores persist on hospital surfaces after disinfection. The purposes of this study were to isolate and characterize C. difficile from the environment of a large Texas hospital. Methods We collected 330 swab samples of hospital environmental surfa

APA, Harvard, Vancouver, ISO, and other styles

44

Luyten, Yvette A., Janelle R. Thompson, Wendy Morrill, Martin F. Polz, and Daniel L. Distel. "Extensive Variation in Intracellular Symbiont Community Composition among Members of a Single Population of the Wood-Boring Bivalve Lyrodus pedicellatus (Bivalvia: Teredinidae)." Applied and Environmental Microbiology 72, no. 1 (2006): 412–17. http://dx.doi.org/10.1128/aem.72.1.412-417.2006.

Full text

Abstract:

ABSTRACT Shipworms (wood-boring bivalves of the family Teredinidae) harbor in their gills intracellular bacterial symbionts thought to produce enzymes that enable the host to consume cellulose as its primary carbon source. Recently, it was demonstrated that multiple genetically distinct symbiont populations coexist within one shipworm species, Lyrodus pedicellatus. Here we explore the extent to which symbiont communities vary among individuals of this species by quantitatively examining the diversity, abundance, and pattern of occurrence of symbiont ribotypes (unique 16S rRNA sequence types) a

APA, Harvard, Vancouver, ISO, and other styles

45

Dawson, Lisa F., Esmeralda Valiente, Elizabeth H. Donahue, George Birchenough, and Brendan W. Wren. "Hypervirulent Clostridium difficile PCR-Ribotypes Exhibit Resistance to Widely Used Disinfectants." PLoS ONE 6, no. 10 (2011): e25754. http://dx.doi.org/10.1371/journal.pone.0025754.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Alcides, Ana P. P., Jon S. Brazier, Leandro J. F. Pinto, et al. "New PCR ribotypes of Clostridium difficile detected in children in Brazil." Antonie van Leeuwenhoek 92, no. 1 (2007): 53–59. http://dx.doi.org/10.1007/s10482-006-9134-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Alam, M. Jahangir, Khurshida Begum, Tasnuva Rashid, et al. "First Environmental Investigation of Toxigenic Clostridium difficile at a Large Hospital in Bangladesh." Open Forum Infectious Diseases 4, suppl_1 (2017): S406. http://dx.doi.org/10.1093/ofid/ofx163.1015.

Full text

Abstract:

Abstract Background Toxigenic Clostridium difficile is the most common cause of infectious diarrhea in hospitalized patients in the developed world and an emerging pathogen in developing countries due to increased use of broad-spectrum antibiotics. Although likely ubiquitous worldwide, the prevalence of toxigenic C. difficile spores in the hospital environs of developing countries is poorly understood. The objectives of the study are to isolate and characterize C. difficile from the hospital environs of a large hospitals in Dhaka, Bangladesh. Methods As part of our environmental surveillance e

APA, Harvard, Vancouver, ISO, and other styles

48

Faruque, Shah M., A. K. Siddique, Manujendra N. Saha, et al. "Molecular Characterization of a New Ribotype ofVibrio cholerae O139 Bengal Associated with an Outbreak of Cholera in Bangladesh." Journal of Clinical Microbiology 37, no. 5 (1999): 1313–18. http://dx.doi.org/10.1128/jcm.37.5.1313-1318.1999.

Full text

Abstract:

Vibrio cholerae O139 Bengal initially appeared in the southern coastal region of Bangladesh and spread northward, causing explosive epidemics during 1992 and 1993. The resurgence of V. cholerae O139 during 1995 after its transient displacement by a new clone of El Tor vibrios demonstrated rapid changes in the epidemiology of cholera in Bangladesh. A recent outbreak of cholera in two north-central districts of Bangladesh caused by V. cholerae O139 led us to analyze strains collected from the outbreak and compare them with V. cholerae O139 strains isolated from other regions of Bangladesh and ne

APA, Harvard, Vancouver, ISO, and other styles

49

Hargreaves, K. R., H. V. Colvin, K. V. Patel, J. J. P. Clokie, and M. R. J. Clokie. "Genetically Diverse Clostridium difficile Strains Harboring Abundant Prophages in an Estuarine Environment." Applied and Environmental Microbiology 79, no. 20 (2013): 6236–43. http://dx.doi.org/10.1128/aem.01849-13.

Full text

Abstract:

ABSTRACTClostridium difficileis the leading cause of antibiotic-associated diarrheal disease in health care settings across the world. Despite its pathogenic capacity, it can be carried asymptomatically and has been found in terrestrial and marine ecosystems outside hospital environments. Little is known about these environmental strains, and few studies have been conducted on estuarine systems. Although prophage abundance and diversity are known to occur within clinical strains, prophage carriage within environmental strains ofC. difficilehas not previously been explored. In this study, we is

APA, Harvard, Vancouver, ISO, and other styles

50

Norton, Dawn M., Meghan A. McCamey, Kenneth L. Gall, Janet M. Scarlett, Kathryn J. Boor, and Martin Wiedmann. "Molecular Studies on the Ecology of Listeria monocytogenes in the Smoked Fish Processing Industry." Applied and Environmental Microbiology 67, no. 1 (2001): 198–205. http://dx.doi.org/10.1128/aem.67.1.198-205.2001.

Full text

Abstract:

ABSTRACT We have applied molecular approaches, including PCR-based detection strategies and DNA fingerprinting methods, to study the ecology ofListeria monocytogenes in food processing environments. A total of 531 samples, including raw fish, fish during the cold-smoking process, finished product, and environmental samples, were collected from three smoked fish processing facilities during five visits to each facility. A total of 95 (17.9%) of the samples tested positive forL. monocytogenes using a commercial PCR system (BAX for Screening/Listeria monocytogenes), including 57 (27.7%) environme

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!