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Journal articles on the topic 'PCR-us'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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1

Shin, H. J., G. Rajashekara, F. F. Jirjis, et al. "Specific detection of avian pneumovirus (APV) US isolates by RT-PCR." Archives of Virology 145, no. 6 (2000): 1239–46. http://dx.doi.org/10.1007/s007050070123.

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2

Evans, Andrew, Patsy Whelehan, Alastair Thompson, et al. "Prediction of Pathological Complete Response to Neoadjuvant Chemotherapy for Primary Breast Cancer Comparing Interim Ultrasound, Shear Wave Elastography and MRI." Ultraschall in der Medizin - European Journal of Ultrasound 39, no. 04 (2017): 422–31. http://dx.doi.org/10.1055/s-0043-111589.

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Abstract Background Prediction of pathological complete response (pCR) of primary breast cancer to neoadjuvant chemotherapy (NACT) may influence planned surgical approaches in the breast and axilla. The aim of this project is to assess the value of interim shear wave elastography (SWE), ultrasound (US) and magnetic resonance imaging (MRI) after 3 cycles in predicting pCR. Methods 64 patients receiving NACT had baseline and interim US, SWE and MRI examinations. The mean lesion stiffness at SWE, US and MRI diameter was measured at both time points. We compared four parameters with pCR status: a)
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3

Evans, Andrew, Patsy Whelehan, Alastair Thompson, et al. "Prediction of pathological complete response to neoadjuvant chemotherapy for primary breast cancer comparing interim ultrasound, Shear Wave elastography and MRI." Senologie - Zeitschrift für Mammadiagnostik und -therapie 15, no. 04 (2018): 229–37. http://dx.doi.org/10.1055/a-0797-4532.

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Abstract Background Prediction of pathological complete response (pCR) of primary breast cancer to neoadjuvant chemotherapy (NACT) may influence planned surgical approaches in the breast and axilla. The aim of this project is to assess the value of interim shear wave elastography (SWE), ultrasound (US) and magnetic resonance imaging (MRI) after 3 cycles in predicting pCR. Methods 64 patients receiving NACT had baseline and interim US, SWE and MRI examinations. The mean lesion stiffness at SWE, US and MRI diameter was measured at both time points. We compared four parameters with pCR status: a)
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4

Dar, Arshud M., Kathy Tune, Shirin Munir, Brundaban Panigrahy, Sagar M. Goyal, and Vivek Kapur. "PCR-Based Detection of an Emerging Avian Pneumovirus in US Turkey Flocks." Journal of Veterinary Diagnostic Investigation 13, no. 3 (2001): 201–5. http://dx.doi.org/10.1177/104063870101300303.

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5

Lee, Su Hyun, Jung Min Chang, Nariya Cho, and Woo Kyung Moon. "Shear-wave elastography in detection of residual breast cancer after neoadjuvant chemotherapy." Journal of Clinical Oncology 32, no. 26_suppl (2014): 102. http://dx.doi.org/10.1200/jco.2014.32.26_suppl.102.

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102 Background: To evaluate the accuracy of shear-wave elastography (SWE) in detecting residual cancer after neoadjuvant chemotherapy. Methods: This retrospective study was approved by our institutional review board and the requirement for written informed consent was waived. From January 2012 to February 2013, 71 women with stage II-III invasive breast cancers who received neoadjuvant chemotherapy and were imaged with B-mode ultrasonography (US), SWE, and magnetic resonance (MR) imaging before surgery were included. Presence of residual cancer was evaluated and categorized according to the mo
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6

Voit, C. A., G. Schaefer, A. Schoengen, et al. "Ultrasound (US) and US-guided fine needle aspiration cytology (FNAC) prior to sentinel lymph node biopsy (SLNB) in melanoma patients: Accuracy of US-FNAC and lack of further improvement by RT-PCR of the aspirate." Journal of Clinical Oncology 24, no. 18_suppl (2006): 8059. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.8059.

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8059 Background: We have previously shown that US guided FNAC identifies positive SNs prior to SLND in 19% of cases (Voit et al., Ann Surg Oncol: 2006, in press). Originally, in this prospective study, we had tested the feasibility of in vivo US as screening tool and FNAC for verification of sentinel nodes (SN) prior to SLNB. Here we report the results of tyrosinase RT-PCR examinations of SNs aspirated pre-SLNB and of post-SLNB FNAC of the excised SN. Additionally, pts were followed up by blood sample tyrosinase RT-PCR at regular intervals. Methods: Between 7/2001 and 7/2003, 127 AJCC staged m
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Voit, Christiane A., Gregor Schäfer-Hesterberg, Martina Kron, et al. "Impact of Molecular Staging Methods in Primary Melanoma: Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) of Ultrasound-Guided Aspirate of the Sentinel Node Does Not Improve Diagnostic Accuracy, But RT-PCR of Peripheral Blood Does Predict Survival." Journal of Clinical Oncology 26, no. 35 (2008): 5742–47. http://dx.doi.org/10.1200/jco.2007.13.7653.

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Purpose This study analyzes (1) the value of tyrosinase reverse-transcriptase polymerase chain reaction (RT-PCR) of aspirates obtained by ultrasound-guided fine-needle aspiration cytology (US-FNAC) of sentinel nodes (SNs) in patients with melanoma before sentinel lymph node biopsy (SLNB) and (2) the value of RT-PCR of blood samples of all SLNB patients. Patients and Methods Between 2001 and 2003, 127 patients with melanoma (median Breslow depth, 2.1 mm) underwent SLNB. FNAC was performed in all SNs of all patients pre- and post-SLNB. The aspirates were partly shock-frozen for RT-PCR and were p
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8

Alberto Menezes, Carlos, Giovanna Lúcia Oliveira Bonina Costa, Rafael Ferreira Barreto, and Victória Santos Oliveira. "Proteína C reativa importante biomarcador de risco cardiometabólico na obesidade infanto-juvenil." Saúde Coletiva (Barueri) 11, no. 65 (2021): 5882–95. http://dx.doi.org/10.36489/saudecoletiva.2021v11i65p5882-5895.

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Estabelecer a importância da avaliação da proteína C reativa ultrassensível (PCR-us) como biomarcador em um grupo pediátrico obeso, detectando precocemente possíveis complicações cardiometabólicas. Trata-se de estudo caso-controle envolvendo 342 crianças e adolescentes, do Serviço de Medicina Preventiva, Aracaju-Sergipe, Brasil. Participaram do estudo 235 obesos e 107 controles. A PCR-us apresentou valor médio de 2,36 ± 1,28 mg/dL no grupo obeso e 0,01 ± 0,1 mg/dL no grupo controle. Observou-se correlação significativa do aumento de PCR-us no grupo obeso com achados bioquímicos e antropométric
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CASTRO, Kamila Almeida de, and Eliene da Silva Martins VIANA. "Avaliação da proteína C reativa ultrassensível em ratos diabéticos tratados com resveratrol." Revista Eletrônica Científica da UERGS 4, no. 1 (2018): 03–16. http://dx.doi.org/10.21674/2448-0479.41.03-16.

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Pesquisas têm demonstrado o importante papel do resveratrol, na prevenção de doenças, principalmente aquelas ligadas ao sistema cardiovascular. Este estudo teve como objetivo a avaliação da ação do resveratrol sobre a PCR-us e a variação da glicemia em ratos diabéticos. No estudo foram utilizados 24 ratos machos, Wistar, adultos e divididos em 4 grupos: 1: Controle: Animais saudáveis, sem nenhum tratamento especial; 2: Animais diabéticos não tratados; 3: Animais diabéticos tratados com hipoglicemiante (Glibenclamida) e 4: Animais diabéticos tratados com Resveratrol. Observou-se que o grupo de
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10

Bando, H., M. Ishii, E. Tohno, and E. Ueno. "Real-time ultrasound elastography for monitoring tumor response to neoadjuvant chemotherapy in primary breast cancer." Journal of Clinical Oncology 25, no. 18_suppl (2007): 14023. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.14023.

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14023 Background: Response to neoadjuvant treatment is vital to predict a patient’s long-term survival. Precise detection of residual tumor cells after neoadjuvant chemotherapy would allow a better cosmetic results avoiding over surgery and reduce second operation due to positive margin status. Moreover, accurate prediction of pathological CR will yield no surgical intervention in certain population. Recently, a new generation of ultrasound platforms with real-time freehand elastography that enables the imaging of elasticity of the lesion by using the extended combined autocorrelation method (
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11

Dean, Christina L., Emily Alvey, Crystal Evans, Charles Hill, Eileen Burd, and Colleen Kraft. "Verification of a Novel Multiplex PCR Respiratory Virus Panel in a US Biocontainment Unit." American Journal of Clinical Pathology 152, Supplement_1 (2019): S7. http://dx.doi.org/10.1093/ajcp/aqz112.013.

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Abstract Emerging infectious diseases carry unique logistical, financial, and clinical ramifications. Rapid diagnostic testing methods can alleviate some of these challenges by providing definitive diagnoses earlier in the clinical course, leading to appropriate targeted therapy, cost savings, and improved patient outcomes. The BioFire FilmArray Respiratory Panel 2 plus (RP2plus; bioMérieux, Marcy l’Etoile, France) is a multiplexed nucleic acid test for detection of Middle East respiratory syndrome coronavirus (MERS-CoV) and 14 common viral and 4 bacterial respiratory pathogens in nasopharynge
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12

Cao, Yiqi, Miao Yu, Guihua Dong, Bing Chen, and Baiyu Zhang. "Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation." Molecules 25, no. 3 (2020): 706. http://dx.doi.org/10.3390/molecules25030706.

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Biodegradation of contaminants is extremely complicated due to unpredictable microbial behaviors. Monitoring of microbial biodegradation drives us to determine (1) the amounts of specific degrading microbes, (2) the abundance, and (3) expression level of relevant functional genes. To this endeavor, the cultivation independent polymerase chain reaction (PCR)-based monitoring technique develops from endpoint PCR, real-time quantitative PCR, and then into novel digital PCR. In this review, we introduce these three categories of PCR techniques and summarize the timely applications of digital PCR a
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13

Prakash, Channapatna S., Guohao He, and Robert L. Jarret. "DNA SEQUENCE POLYMORPHISM BASED GENETIC DIVERSITY STUDIES IN SWEETPOTATO GERMPLASM." HortScience 29, no. 7 (1994): 727c—727. http://dx.doi.org/10.21273/hortsci.29.7.727c.

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Highly polymorphic DNA markers were identified in sweetpotato (Ipomoea batatas) using PCR amplification and arbitrary primers. More than 100 accessions representing US cultivars and their progenitors, and germplasm lines from around the world were analyzed. Sweetpotato germplasm exhibited high genetic variability and individual-specific profiles were obtained for all accessions. US cultivars formed a tight cluster in the principal coordinate analysis suggesting a narrow genetic base. The genetic relationship data of US cultivars and their progenitors based on DNA polymorphisms was in agreement
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14

Schönfeld, J., H. Heuer, J. D. van Elsas, and K. Smalla. "Specific and Sensitive Detection of Ralstonia solanacearum in Soil on the Basis of PCR Amplification of fliC Fragments." Applied and Environmental Microbiology 69, no. 12 (2003): 7248–56. http://dx.doi.org/10.1128/aem.69.12.7248-7256.2003.

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ABSTRACT Ralstonia solanacearum is the causative agent of bacterial wilt in many important crops. A specific and sensitive PCR detection method that uses primers targeting the gene coding for the flagella subunit, fliC, was established. Based on the first fliC gene sequence of R. solanacearum strain K60 available at GenBank, the Ral_fliC PCR primer system was designed; this system yielded a single 724-bp product with the DNAs of all of the R. solanacearum strains tested. However, R. pickettii and four environmental Ralstonia isolates also yielded amplicons. The Ral_fliC PCR products obtained w
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15

Martino, Richard J., Kristen D. Krause, Marybec Griffin, et al. "A Nationwide Survey of COVID-19 Testing in LGBTQ+ Populations in the United States." Public Health Reports 136, no. 4 (2021): 493–507. http://dx.doi.org/10.1177/00333549211018190.

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Objectives Lesbian, gay, bisexual, transgender, or queer and questioning (LGBTQ+) people and populations face myriad health disparities that are likely to be evident during the COVID-19 pandemic. The objectives of our study were to describe patterns of COVID-19 testing among LGBTQ+ people and to differentiate rates of COVID-19 testing and test results by sociodemographic characteristics. Methods Participants residing in the United States and US territories (N = 1090) aged ≥18 completed an internet-based survey from May through July 2020 that assessed COVID-19 testing and test results and socio
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16

Aradaib, Imadeldin E., Geoffrey Y. Akita, and Bennie I. Osburn. "Detection of Epizootic Hemorrhagic Disease Virus Serotypes 1 and 2 in Cell Culture and Clinical Samples Using Polymerase Chain Reaction." Journal of Veterinary Diagnostic Investigation 6, no. 2 (1994): 143–47. http://dx.doi.org/10.1177/104063879400600202.

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The potential for the use of the polymerase chain reaction (PCR) in detecting epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell cultures and clinical samples was studied. Using oligoribonucleotide primers selected from genome segment 6 of EHDV-2 (Alberta strain), the PCR-based assay resulted in a 387-base pair (bp) PCR product. EHDV RNA from US prototype serotypes 1 and 2 and a number of EHDV field isolates, propagated in cell cultures, were detected by this EHDV PCR-based assay. Amplification products were visualized on ethidium bromide-stained agarose gels or detected by ch
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17

Ooteman, Márcia Costa, Annamaria Ravara Vago, and Matilde Cota Koury. "Potential application of low-stringency single specific primer-PCR in the identification ofLeptospirain the serum of patients with suspected leptospirosis." Canadian Journal of Microbiology 50, no. 12 (2004): 1073–79. http://dx.doi.org/10.1139/w04-096.

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In this study we tested the potential use of low-stringency single specific primer-PCR (LSSP-PCR) for genetically typing Leptospira directly from biological samples. Serum samples obtained from 29 patients with clinically suspected leptospirosis were amplified by specific PCR, using the previously selected G1 and G2 primers. The PCR products of approximately 300 bp were subsequently used as a template for LSSP-PCR analysis. We were able to produce genetic signatures from the leptospires present in the human samples, which permitted us to make a preliminary identification of the infective serov
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18

Haase, Antje, Maree Brennan, Siobhan Barrett, et al. "Evaluation of PCR for Diagnosis of Melioidosis." Journal of Clinical Microbiology 36, no. 4 (1998): 1039–41. http://dx.doi.org/10.1128/jcm.36.4.1039-1041.1998.

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Previously published PCR-based diagnostic tests for melioidosis were evaluated for clinical usefulness. A Burkholderia pseudomallei 16S rRNA-derived primer set had a sensitivity approaching 100% for clinical samples from 22 culture-confirmed cases of melioidosis and enabled diagnosis of 3 culture-negative cases. However, samples from 10 of 30 inpatients from Royal Darwin Hospital with other diagnoses were positive by PCR, giving a specificity of 67% and a positive predictive value of only 70%. Although there are a number of intriguing possible explanations for our results, concerns of inapprop
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Baele, Margo, Paul Baele, Mario Vaneechoutte, et al. "Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species." Journal of Clinical Microbiology 38, no. 11 (2000): 4201–7. http://dx.doi.org/10.1128/jcm.38.11.4201-4207.2000.

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tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faeciumshowed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highl
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20

Etievant, Sibyle, Antonin Bal, Vanessa Escuret, et al. "Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories." Journal of Clinical Medicine 9, no. 6 (2020): 1871. http://dx.doi.org/10.3390/jcm9061871.

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A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charité (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory
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Frickmann, Hagen, Norbert G. Schwarz, Raphael Rakotozandrindrainy, Jürgen May, and Ralf M. Hagen. "PCR for enteric pathogens in high-prevalence settings. What does a positive signal tell us?" Infectious Diseases 47, no. 7 (2015): 491–98. http://dx.doi.org/10.3109/23744235.2015.1022212.

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22

Sinha, Avanti, Phillip Gauger, Jianqiang Zhang, Kyoung-Jin Yoon, and Karen Harmon. "PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine." Veterinary Microbiology 179, no. 3-4 (2015): 296–98. http://dx.doi.org/10.1016/j.vetmic.2015.06.005.

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23

Liu, Xinsheng, and Qiuhong Wang. "Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains." Journal of Virological Methods 234 (August 2016): 137–41. http://dx.doi.org/10.1016/j.jviromet.2016.04.018.

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24

Lalam, N., C. Jacob, and P. Jagers. "Modelling the PCR amplification process by a size-dependent branching process and estimation of the efficiency." Advances in Applied Probability 36, no. 02 (2004): 602–15. http://dx.doi.org/10.1017/s0001867800013628.

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We propose a stochastic modelling of the PCR amplification process by a size-dependent branching process starting as a supercritical Bienaymé-Galton-Watson transient phase and then having a saturation near-critical size-dependent phase. This model allows us to estimate the probability of replication of a DNA molecule at each cycle of a single PCR trajectory with a very good accuracy.
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Lalam, N., C. Jacob, and P. Jagers. "Modelling the PCR amplification process by a size-dependent branching process and estimation of the efficiency." Advances in Applied Probability 36, no. 2 (2004): 602–15. http://dx.doi.org/10.1239/aap/1086957587.

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We propose a stochastic modelling of the PCR amplification process by a size-dependent branching process starting as a supercritical Bienaymé-Galton-Watson transient phase and then having a saturation near-critical size-dependent phase. This model allows us to estimate the probability of replication of a DNA molecule at each cycle of a single PCR trajectory with a very good accuracy.
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26

MONTEIRO, Mussara Gomes Cavalcanti Alves, Yohanna De OLIVEIRA, Rafaella Cristhine Pordeus LUNA, et al. "EXISTE RELAÇÃO ENTRE NÍVEIS DE RETINOL SÉRICO, INGESTÃO DE FIBRA E PROTEÍNA C-REATIVA ULTRA-SENSÍVEL EM IDOSOS HIPERTENSOS?" Revista Brasileira de Ciências da Saúde 22, no. 2 (2018): 173–80. http://dx.doi.org/10.22478/ufpb.2317-6032.2018v22n2.35653.

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Objetivo: O objetivo deste estudo foi investigar a associação do retinol sérico, proteína C-reativa ultra-sensível (PCR-us) e a ingestão de fibras alimentares em uma população de idosos hipertensos. Material e Métodos: Trata-se de um estudo transversal de base populacional com 170 idosos com idade entre 60 e 90 anos, de ambos os sexos, de uma cidade do Nordeste do Brasil. Para as análises bioquímicas, as concentrações de retinol sérico e PCR-us foram analisadas e um questionário quantitativo de frequência de alimentar auto-administrado foi coletado. Foram utilizados como critérios de inclusão:
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27

Ambrose, Christopher S., Lisa L. Steed, Michael Brandon, Kara Frye, and Gina Thomson. "728. Regional Validation of Distinct RSV Seasonality Thresholds for Antigen and PCR Testing." Open Forum Infectious Diseases 5, suppl_1 (2018): S261—S262. http://dx.doi.org/10.1093/ofid/ofy210.735.

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Abstract Background Respiratory syncytial virus (RSV) produces annual epidemics that vary in the timing of season onset, peak, and duration by season and by geographic region. Recent analyses by the US Centers for Disease Control at the national level have demonstrated that polymerase chain reaction (PCR) testing has largely replaced rapid antigen testing as the predominant test type and that the traditional 10% positivity threshold for defining an RSV season based on antigen testing should not be applied to PCR testing, for which the comparable threshold for real-time surveillance was 3%. The
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Ts, Alimaa, Zolzaya M, and Yoshio Nakamura. "Development of Parasitemia and anemia caused by T. Orientalis infection in experimental calves." Mongolian Journal of Agricultural Sciences 24, no. 02 (2018): 3–8. http://dx.doi.org/10.5564/mjas.v24i02.1109.

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In the present study, we have studied development of parasitemia and anemia which caused by T. orientalis infection in experimental calves using different kind of laboratory tests such us blood smear test, blood cell examination test and conventional PCR. The blood smear test is still one of easiest, effective diagnostic methods. According to our results the sensitivity of this test was as same as PCR.
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Puel, O., F. Galgani, C. Dalet, and P. Lassus. "Partial sequence of the 24S rRNA and polymerase chain reaction based assay for the toxic dinoflagellateDinophysis acuminata." Canadian Journal of Fisheries and Aquatic Sciences 55, no. 3 (1998): 597–604. http://dx.doi.org/10.1139/f97-288.

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We describe the polymerase chain reaction (PCR) amplification of an 805 base pair fragment of 24S rRNA from the toxic dinoflagellate Dinophysis acuminata and the sequence of this fragment. We also describe a PCR-based assay for the specific detection of D. acuminata in seawater samples. Conserved primers, starting at positions 711 and 1489 of the 24S rRNA from the dinoflagellate Prorocentrum micans, were used for the PCR. The PCR product was cloned and sequenced. The fragment was aligned with rRNA sequences from other protists. Two oligonucleotides in variable domains of the sequence from D. a
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Acosta, Fermín, Ana Fernández-Cruz, Sandra R. Maus, et al. "In-Depth Study of a Nosocomial Outbreak Caused by Extensively Drug-Resistant Pseudomonas aeruginosa Using Whole Genome Sequencing Coupled With a Polymerase Chain Reaction Targeting Strain-Specific Single Nucleotide Polymorphisms." American Journal of Epidemiology 189, no. 8 (2020): 841–49. http://dx.doi.org/10.1093/aje/kwaa025.

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Abstract In 2013–2014, an outbreak involving 14 patients infected by an extensively drug-resistant strain of Pseudomonas aeruginosa was detected in a hospital in Madrid, Spain. Our objective was to evaluate an alternative strategy for investigating the outbreak in depth by means of molecular and genomic approaches. Pulsed-field gel electrophoresis (PFGE) was applied as a first-line approach, followed by a more refined whole genome sequencing analysis. Single nucleotide polymorphisms identified by whole genome sequencing were used to design a specific polymerase chain reaction (PCR) for screeni
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Roman, Véronica L., Christophe Merlin, Marko P. J. Virta, and Xavier Bellanger. "EpicPCR 2.0: Technical and Methodological Improvement of a Cutting-Edge Single-Cell Genomic Approach." Microorganisms 9, no. 8 (2021): 1649. http://dx.doi.org/10.3390/microorganisms9081649.

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EpicPCR (Emulsion, Paired Isolation and Concatenation PCR) is a recent single-cell genomic method based on a fusion-PCR allowing us to link a functional sequence of interest to a 16S rRNA gene fragment and use the mass sequencing of the resulting amplicons for taxonomic assignment of the functional sequence-carrying bacteria. Although it is interesting because it presents the highest efficiency for assigning a bacterial host to a marker, epicPCR remains a complex multistage procedure with technical difficulties that may easily impair the approach depth and quality. Here, we described how to ad
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Andrade, Marcio M., Vanesa Andreu, Javier Gervas, M. Angeles Montañes, Gloria Soro, and Pilar Giraldo. "TKI Suspension in CML, the New Challenge for Hematologist. Would Ddpcr Help Us to Improve Outcomes?" Blood 124, no. 21 (2014): 5512. http://dx.doi.org/10.1182/blood.v124.21.5512.5512.

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Abstract Background: For molecular assessment in CML, NCCN and European LeukemiaNet had defined the cut-off values. Until now the gold standard for BCR-ABL quantification is the real time quantitative polymerase chain reaction (RQ-PCR), and this technique has a limited sensibility to MR4.0 or MR4.5 in the majority of laboratories worldwide. There are several discontinuation studies for CP-CML patients under imatinib therapy who achieved and sustained at least MR4.0 during 2 years or more, and the fact of achieve a deeper response and the time during this response had been the major factors for
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Ben, Mirian Dal, Maura Oliveira, Paola Cappellano, Jorge Sampaio, and Maria Beatriz Dias. "Evaluating the Cost-Effectiveness of Proposed Algorithms for C. difficile Infection in Different Pretest Probability Settings." Infection Control & Hospital Epidemiology 41, S1 (2020): s224—s225. http://dx.doi.org/10.1017/ice.2020.769.

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Background: The use of real-time polymerase chain reaction (RT-PCR) as a first-line test for the diagnosis of Clostridioides difficile may result in overdiagnosis and overtreatment because the test is not capable of distinguishing infection from carriage. Toxin EIA assays have impeditive low sensitivity. Some algorithms using enzyme immunoassay for glutamate dehydrogenase (GDH) antigen and toxins A and B as the first step have been proposed to increase diagnostic performance. However, cost-effectiveness of different diagnostic algorithms would depend on the cost of each test and on the pretest
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Lewin, S. R., M. Vesanen, L. Kostrikis, et al. "Use of Real-Time PCR and Molecular Beacons To Detect Virus Replication in Human Immunodeficiency Virus Type 1-Infected Individuals on Prolonged Effective Antiretroviral Therapy." Journal of Virology 73, no. 7 (1999): 6099–103. http://dx.doi.org/10.1128/jvi.73.7.6099-6103.1999.

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ABSTRACT We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antivira
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35

Ferdinands, Jill M., Lauren E. W. Olsho, Anna A. Agan, et al. "Effectiveness of Influenza Vaccine Against Life-threatening RT-PCR-confirmed Influenza Illness in US Children, 2010–2012." Journal of Infectious Diseases 210, no. 5 (2014): 674–83. http://dx.doi.org/10.1093/infdis/jiu185.

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36

Sen, K., and M. Rodgers. "Distribution of six virulence factors in Aeromonas species isolated from US drinking water utilities: a PCR identification." Journal of Applied Microbiology 97, no. 5 (2004): 1077–86. http://dx.doi.org/10.1111/j.1365-2672.2004.02398.x.

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37

Muck, Richard. "Recent advances in silage microbiology." Agricultural and Food Science 22, no. 1 (2013): 3–15. http://dx.doi.org/10.23986/afsci.6718.

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Recent advances in silage microbiology are reviewed. Most new techniques in silage microbiology use the polymerase chain reaction (PCR) to make copies of a portion of the DNA in microorganisms. These techniques allow us to identify and quantify species as well as do community analysis. The PCR-based techniques are uncovering new species, both bacteria and fungi, during storage and feeding. Silage inoculants are widely available, but of greater interest has been research investigating why inoculants are so successful. Various inoculant strains have been found to produce bacteriocins and other c
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38

Reboud, Julien, Yannyk Bourquin, Rab Wilson, et al. "Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies." Proceedings of the National Academy of Sciences 109, no. 38 (2012): 15162–67. http://dx.doi.org/10.1073/pnas.1206055109.

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Ultrasonics offers the possibility of developing sophisticated fluid manipulation tools in lab-on-a-chip technologies. Here we demonstrate the ability to shape ultrasonic fields by using phononic lattices, patterned on a disposable chip, to carry out the complex sequence of fluidic manipulations required to detect the rodent malaria parasite Plasmodium berghei in blood. To illustrate the different tools that are available to us, we used acoustic fields to produce the required rotational vortices that mechanically lyse both the red blood cells and the parasitic cells present in a drop of blood.
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39

Gonzalo-Asensio, Jesús, Carlos Y. Soto, Ainhoa Arbués, et al. "The Mycobacterium tuberculosis phoPR Operon Is Positively Autoregulated in the Virulent Strain H37Rv." Journal of Bacteriology 190, no. 21 (2008): 7068–78. http://dx.doi.org/10.1128/jb.00712-08.

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ABSTRACT The attenuated Mycobacterium tuberculosis H37Ra strain is an isogenic counterpart of the virulent paradigm strain H37Rv. Recently, a link between a point mutation in the PhoP transcriptional regulator and avirulence of H37Ra was established. Remarkably, a previous study demonstrated negative autoregulation of the phoP gene in H37Ra. These findings led us to study the transcriptional autoregulation of PhoP in the virulent H37Rv strain. In contrast to the negative autoregulation of PhoP previously published for H37Ra, our experiments using a phoP promoter-lacZ fusion showed that PhoP is
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40

Erice, Alejo, Donald Brambilla, James Bremer, et al. "Performance Characteristics of the QUANTIPLEX HIV-1 RNA 3.0 Assay for Detection and Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma." Journal of Clinical Microbiology 38, no. 8 (2000): 2837–45. http://dx.doi.org/10.1128/jcm.38.8.2837-2845.2000.

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The QUANTIPLEX HIV-1 RNA assay, version 3.0 (a branched DNA, version 3.0, assay [bDNA 3.0 assay]), was evaluated by analyzing spiked and clinical plasma samples and was compared with the AMPLICOR HIV-1 MONITOR Ultrasensitive (ultrasensitive reverse transcription-PCR [US-RT-PCR]) method. A panel of spiked plasma samples that contained 0 to 750,000 copies of human immunodeficiency virus type 1 (HIV-1) RNA per ml was tested four times in each of four laboratories (1,344 assays). Negative results (<50 copies/ml) were obtained in 30 of 32 (94%) assays with seronegative samples, 66 of 128 (52%) a
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41

Sechler, K. E., M. M. Carras, N. Shishkoff, and P. W. Tooley. "Adaptation of a Phytophthora ramorum Real-Time Polymerase Chain Reaction Assay Based on a Mitochondrial Gene Region for Use on the Cepheid SmartCycler." Plant Health Progress 11, no. 1 (2010): 28. http://dx.doi.org/10.1094/php-2010-0212-01-rs.

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Detection of Phytophthora ramorum in US commercial nurseries has led to a number of quarantine regulations. Methods such as real-time PCR (RT-PCR) provide rapid and reliable detection that can supplement attempts to culture P. ramorum from symptomatic tissue. We adapted and optimized a previously described mitochondrial gene-based RT-PCR assay for use with a Cepheid SmartCycler v.1 and ready-to-use lyophilized PCR beads. The detection limit was 10 fg of P. ramorum genomic DNA. No cross-reactivity was observed on the SmartCycler for seven additional Phytophthora species tested, which included s
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42

Qian, Wenbin, Junqing Liu, and Jie Jin. "Detection of Aspergillus Species in Blood of Malignant Hemotopoietic Tumor Patients by Two-Step PCR." Blood 106, no. 11 (2005): 4626. http://dx.doi.org/10.1182/blood.v106.11.4626.4626.

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Abstract Objective To investigate the sensitivity and specificity of two-step PCR in detection of aspergillus species in blood from malignant hemotopoietic tumor patients. Methods Forty-one blood samples from patients at high-risk for invasive fungal disease were detected by two-step PCR. The clinic applicability of two-step PCR for systemic aspergillosis diagnosis was assessed by analyzing computed tomography, aspergillus culture, neutropenia and outcome after anti-fungal therapy. Results Specific band of PCR was obtained from aspergillus strains and the products of PCR had a high homogeneous
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43

Sarkar, Arindam, Debjani Taraphdar, and Shyamalendu Chatterjee. "Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010." Journal of Tropical Medicine 2012 (2012): 1–5. http://dx.doi.org/10.1155/2012/960329.

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Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different sero
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44

Puerto de Amaya, Miryam B., Mercedes Olaya Contreras, Karen Rey Cediel, Katherinne Diaz Jiménez, and Carlos H. Pérez M. "Frecuencia de DNA-HPV-H en células escamosas atípicas. Serie de casos: ASC-US y LSIL." Revista Repertorio de Medicina y Cirugía 23, no. 2 (2014): 121–26. http://dx.doi.org/10.31260/repertmedcir.v23.n2.2014.726.

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Objetivo: determinar mediante reacción en cadena de la polimerasa (PCR) la infección por el virus del papiloma humano de alto riesgo (DNA-HPV-H) y la presencia de cambio morfológico con atipia en la citología de mujeres que laboran en un hospital y un ente educativo. Métodos: serie de casos de muestras cérvico uterinas con citologías convencional y en base líquida, y PCR para DNA-HPV-H; se incluyeron las pacientes que tuvieron uno o más resultados positivos en citología (ASC-US y LSIL). Resultados: la tipificación de DNA-HPV-H fue positiva en 12 de 41 casos. Se observó un mayor número de citol
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45

Colina Cifuentes, Rosa, Rommel Espinoza De los Monteros, James Franco, Bolívar Saenz, and Luis Alejandro Cárdenas. "Relación entre cardiopatía isquémica y la proteína C reactiva." Revista Ecuatoriana de Medicina y Ciencias Biológicas 36, no. 1-2 (2017): 9–15. http://dx.doi.org/10.26807/remcb.v36i1-2.259.

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Las enfermedades cardiovasculares están relacionadas con procesos inflamatorios prolongados o persistentes que causan el aumento de los niveles de reactantes de fase aguda como la proteína C reactiva (PCR), el fibrinógeno y las interleucinas IL-1 e IL-6. Desde este punto es importante saber si existen en los ecuatorianos polimorfismos genéticos ya establecidos para otras poblaciones, en especial en las citosinas IL-1 e IL-6. El objetivo del estudio fue relacionar la proteína C reactiva ultrasensible (PCR-us) en pacientes con cardiopatía isquémica. Se analizaron un total de 91 personas: 50 paci
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46

Colina Cifuentes, Rosa, Rommel Espinoza de los Monteros, James Franco, Bolívar Saenz, and Luis Alejandro Cárdenas. "Relación entre cardiopatía isquémica y la proteína C reactiva." Revista Ecuatoriana de Medicina y Ciencias Biológicas 36, no. 1-2 (2019): 9–15. http://dx.doi.org/10.26807/remcb.v36i1-2.59.

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Las enfermedades cardiovasculares están relacionadas con procesos inflamatorios prolongados o persistentes que causan el aumento de los niveles de reactantes de fase aguda como la proteína C reactiva (PCR), el fibrinógeno y las interleucinas IL-1 e IL-6. Desde este punto es importante saber si existen en los ecuatorianos polimorfismos genéticos ya establecidos para otras poblaciones, en especial en las citosinas IL-1 e IL-6. El objetivo del estudio fue relacionar la proteína C reactiva ultrasensible (PCR-us) en pacientes con cardiopatía isquémica. Se analizaron un total de 91 personas: 50 paci
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47

Guillard, Thomas, Anne-Laure Lebreil, Lars Hestbjerg Hansen, et al. "Discrimination between Native and Tn6010-AssociatedoqxABin Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy." Antimicrobial Agents and Chemotherapy 59, no. 9 (2015): 5838–40. http://dx.doi.org/10.1128/aac.00669-15.

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ABSTRACTWe developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associatedoqxABinEnterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find thatKlebsiellasp. andRaoultellasp. reference strains chromosomally carriedoqxAB.
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48

Valera, Juliana M., Tatyana Diaz, Lauren E. Petty, et al. "Prevalence of spinocerebellar ataxia 36 in a US population." Neurology Genetics 3, no. 4 (2017): e174. http://dx.doi.org/10.1212/nxg.0000000000000174.

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Objective:To assess the prevalence and clinical features of individuals affected by spinocerebellar ataxia 36 (SCA36) at a large tertiary referral center in the United States.Methods:A total of 577 patients with undiagnosed sporadic or familial cerebellar ataxia comprehensively evaluated at a tertiary referral ataxia center were molecularly evaluated for SCA36. Repeat primed PCR and fragment analysis were used to screen for the presence of a repeat expansion in the NOP56 gene.Results:Fragment analysis of triplet repeat primed PCR products identified a GGCCTG hexanucleotide repeat expansion in
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49

Dankevych, L. A. "PCR-identification of bacteria belonging to the genus Pectobacterium – agents of cucumber soft rot and wilting in Ukraine." Faktori eksperimental'noi evolucii organizmiv 27 (September 1, 2020): 61–65. http://dx.doi.org/10.7124/feeo.v27.1303.

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Aim. Correct species identification of isolated Pectobacterium sp., collection «E. toxica» strains and typical representatives of some species of the genus Pectobacterium and Dickeya via PCR for individual species-specific regions of their genome. Methods. Microbiological and molecular genetic (PCR) methods Results. A specific PCR product of size 434 bp was amplified in the genome of isolated Pectobacterium sp., collection «E. toxica» and typical P. carotovorum susp. carotovorum UKM B1075T and P. atrosepticum UKM B-1084T strains. The 690 bp DNA fragment was detected solely in the genome of the
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50

Chouaia, Bessem, Paolo Rossi, Matteo Montagna, et al. "Molecular Evidence for Multiple Infections as Revealed by Typing of Asaia Bacterial Symbionts of Four Mosquito Species." Applied and Environmental Microbiology 76, no. 22 (2010): 7444–50. http://dx.doi.org/10.1128/aem.01747-10.

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ABSTRACT The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with four mosquito species, Anopheles stephensi, Anopheles gambiae, Aedes aegypti, and Aedes albopictus, which are important vectors of human and/or animal pathogens. Denaturing gradient gel electrophoresis (DG
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