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Dissertations / Theses on the topic 'PCR2'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 March 2023

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1

Caye-Eude, Aurélie. "Génétique et architecture clonale des leucémies myélomonocytaires juvéniles sporadiques et syndromiques." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC173/document.

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La LMMJ est un syndrome myéloprolifératif et myélodysplasique rare du jeune enfant, initiée par des mutations classiquement décrites comme mutuellement exclusives de RAS (NRAS, KRAS) ou de régulateurs de la voie RAS (PTPN11, NF1 ou CBL). Ces mutations, somatiques ou constitutionnelles, entraînent l’hyperactivation de cette voie de signalisation et une hypersensibilité spécifique au GM-CSF. La LMMJ est une hémopathie sévère dont le seul traitement est l’allogreffe de moelle osseuse. Cependant sa présentation et son évolution sont particulièrement hétérogènes puisqu’une transformation en leucémi
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LOMBINO, Jessica. "RATIONAL DESIGN AND SYNTHESIS OF NOVEL INHIBITORS OF EPIGENETIC TARGET INVOLVED IN CANCER." Doctoral thesis, Università degli Studi di Palermo, 2021. http://hdl.handle.net/10447/504531.

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Mathieu, Mickael. "Rôle de EZH2 et du complexe PRC2 dans l’homéostasie du cortex surrénalien." Thesis, Université Clermont Auvergne‎ (2017-2020), 2018. http://www.theses.fr/2018CLFAC007/document.

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Les surrénales sont des glandes endocrines permettant la réponse au stress de l’organisme. Alors que la medulla produit des catécholamines, la corticosurrénale sécrète des minéralocorticoïdes au niveau de la zone glomérulée, et des glucocorticoïdes grâce aux cellules de la zone fasciculée. Ces hormones sont notamment impliquées dans l’homéostasie hydrominérale, la réponse immunitaire et la maturation pulmonaire au cours de la vie fœtale. Les insuffisances surrénaliennes peuvent donc être très délétère en absence de traitement. Pour maintenir l’intégrité tissulaire au cours de la vie et pour mi
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Cravello, Laëtitia. "Etudes structurales des protéines par spectrométrie de masse couplée aux échanges hydrogène/deuterium et à la réticulation chimique." Université Joseph Fourier (Grenoble), 2005. https://tel.archives-ouvertes.fr/tel-00009698.

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Les protéines sont impliquées dans de nombreux processus biologiques. Il est nécessaire pour comprendre en détail leur fonction et leur mode d’action afin d’obtenir des informations sur leur structure et sur leurs interactions éventuelles avec leurs partenaires. Le travail réalisé durant cette thèse a consisté à développer deux méthodes innovantes utilisant la spectrométrie de masse pour étudier la structure des protéines et à appliquer ces méthodes à une problématique biologique. Nous avons optimisé une méthode associant les échanges H/D et la spectrométrie de masse sur une protéine modèle, l
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SALES, Tatyane Penha. "Diagnóstico da circovirose suína em criações intensivas no Estado de Goiás." Universidade Federal de Goiás, 2011. http://repositorio.bc.ufg.br/tede/handle/tde/857.

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Made available in DSpace on 2014-07-29T15:07:34Z (GMT). No. of bitstreams: 1 Dissertacao Tatyane P Sales.pdf: 1783169 bytes, checksum: 234cc6cbf4d24d8487721c1017369423 (MD5) Previous issue date: 2011-03-25<br>Circovirosis, a disease associated to porcine circovirus 2 (PCV2), has been clinically reported as postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS) and other diseases presenting reproductive, respiratory and digestive clinical manifestations. Such diseases are considered emergent and economically important for the pig industry. This s
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au, maodea@agric wa gov, and Mark O'Dea. "Pathogenesis and Detection of Porcine Circovirus Type 2 in the Australian Pig Herd." Murdoch University, 2008. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20081128.125816.

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The diagnosis of porcine circovirus-associated disease (PCVAD) in pigs requires the detection of characteristic clinical signs and pathological changes, and the detection of virus in tissues of affected pigs. To increase Australia’s capacity to independently diagnose PCVAD in Australia, techniques for the detection of Porcine circovirus type 2 (PCV2) infection in pigs were developed and are reported in this thesis. These techniques were applied to samples obtained from normal pigs and pigs with disease and confirmed the presence of PCV2 and PCVAD in the Australian pig herd. Viral DNA was de
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Dezen, Diogenes. "Desenvolvimento de uma vacina recombinante para circovirose suína e ensaios para diagnóstico molecular de PCV2." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/32147.

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O circovírus suíno tipo 2 (PCV2) é o principal agente da síndrome multissistêmica do definhamento do suíno (SMDS), uma doença mundialmente disseminada e que provoca perdas econômicas significativas para a suinocultura. Visando contribuir no diagnóstico da síndrome, o presente trabalho padronizou e comparou testes para a detecção do PCV2. Para isso, foram utilizadas as técnicas de amplificação por círculo rolante (ACR) e variações da PCR (convencional, tempo-real e competitiva). Utilizando a ACR foi possível obter a amplificação total de genomas do PCV2, os quais foram clonados, sequenciados e
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BARBOSA, JÚNIOR Sebastião André. "Estudo do Circovírus suíno - 2 (PCV2) pelas técnicas de ELISA indireto por captura de anticorpo e PCR em tempo real em suínos." Universidade Federal Rural de Pernambuco, 2016. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7151.

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Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-02-27T14:16:51Z No. of bitstreams: 1 Sebastiao Andre Barbosa Junior.pdf: 749859 bytes, checksum: e483888ecfa0c1a9815a1e63aaede716 (MD5)<br>Made available in DSpace on 2018-02-27T14:17:28Z (GMT). No. of bitstreams: 1 Sebastiao Andre Barbosa Junior.pdf: 749859 bytes, checksum: e483888ecfa0c1a9815a1e63aaede716 (MD5) Previous issue date: 2016-02-26<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>Porcine circovirus (PCV) belongs to the Circoviridae family and Circovirus genus. The vírus can be subdivided into porcine circ
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Salgado, Vanessa Riesz. "Desenvolvimento de Reações em Cadeia pela Polimerase (PCRs) para o diagnóstico diferencial das principais espécies de Brucella." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-11102012-115215/.

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A brucelose é uma doença altamente contagiosa, responsável por grandes prejuízos econômicos e de saúde pública. É causada por bactérias do gênero Brucella, cujas espécies e seus biovares costumam ser caracterizados pelo isolamento e identificação de características fenotípicas da colônia. Dificuldades como, o perigo na manipulação dos microrganismos, processos laboriosos de tipificação, demora na obtenção de resultados e a instabilidade de características fenotípicas ou isolamento de linhagens atípicas dificultam a tipificação e encorajaram a busca de técnicas mais sensíveis e específicas, com
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Schulze, Esking Wiebke. "Einflussfaktoren bei der Etablierung, Validierung und praktischen Umsetzung von Testverfahren zur Mehrparameterdiagnostik von Infektionskrankheiten beim Schwein am Beispiel von Flüssigchip-Technologie und Multiplex-PCR." Doctoral thesis, Universitätsbibliothek Leipzig, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:15-20081022-142212-0.

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Respiratorische Krankheitsbilder, an denen mehr als ein Pathogen ursächlich beteiligt ist, gewinnen in der Schweinepopulation zunehmend an Bedeutung. Diagnostische Methoden zum simultanen Nachweis mehrerer Erreger sind Bestandteil einer schnellen und effizienten Therapie und tragen zum ökonomischen Bestandsmanagement bei. Im Rahmen dieser Arbeit sollten Methoden für den Multiplex-Nachweis von Antikörpern und Nukleinsäuren viraler Erreger von respiratorischen Krankheitsgeschehen des Schweins entwickelt werden. Die Methode für den Multiplex-Nachweis von Antikörpern sollte auf Basis der xMAP® Flü
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11

Minnella, Walter Settimo Leonardo. "Development of microfluidic tools for biological applications." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0664.

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Cette thèse traite le développement de dispositifs, basés sur la technologie "laboratoire sur puce"(LOC) qui visent à contrôler l'environnement des systèmes biologiques pour des applications macro et microbiologiques. En effet, les caractéristiques de la microfluidique permettent de manipuler l'environnement cellulaire à un niveau supérieur à celui du degré de contrôle atteignable avec les techniques ordinaires. Dans ce travail de thèse sera explorée la possibilité de profiter de ces fonctions afin de développer des outils de diagnostic peu coûteux et pourtant efficaces. En particulier, on rap
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Chisty, L. T. "PcrA function in plasmid replication." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1427879/.

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PcrA is a DNA helicase involved in unwinding plasmi ds as a part of a complex in asymmetric rolling - circle replication of certain plasmids carrying antibiotic resistance genes. PcrA translocates on single stranded DNA by coupling ATP hydrolysis to movement on DNA. Initiator protein, RepD is required to nick supercoiled plasmid site - specifically and open an ssDNA stretch that PcrA can bind. The presence of RepD is needed throughout plasmid unwinding to maintain processivity. Using fluorescent - based techniques, PcrA helicase mechanistic functions and interactions with different components
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Oliver, Ferrando Salvador. "Effect of Porcine circovirus 2 (PCV2) sow or piglet vaccination in different PCV2 subclinical infection scenarios." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/461187.

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La present Tesi doctoral esta formada per tres estudis. El primer estudi va avaluar l'efecte de la vacunació de truges enfront a PCV2 sobre els paràmetres reproductius durant dos cicles gestacionals consecutius. Es van immunitzar 94 truges gestants amb una vacuna comercial enfront a PCV2, i 97 van ser injectades amb una solució salina tamponada amb fosfat (PBS). En el primer cicle reproductiu el tractament va ser aplicat a les 6 i 3 setmanes abans del part, i en el segon cicle es va aplicar una dosi de record o PBS a les 2 setmanes abans del part. Les truges vacunades van mostrar nivells d'ant
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Rozales, Franciéli Pedrotti. "Real time-pcr e nested-pcr no diagnóstico da tuberculose pulmonar." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/72990.

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A tuberculose (TB) é um importante problema de saúde pública, portanto, é necessário o desenvolvimento de novas ferramentas para a detecção rápida e confiável do Mycobacterium tuberculosis, prevenindo a transmissão da TB. O objetivo deste estudo foi avaliar dois testes moleculares para detecção de bactérias do Complexo Mycobacterium tuberculosis (MTBC) diretamente de amostras clínicas. Foi realizado um estudo transversal, no qual foram selecionadas 124 amostras respiratórias. As amostras foram avaliadas por dois testes moleculares “in house” para detecção do MTBC: NESTED-PCR (NPCR) e Real Time
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15

Ziebell, Kim. "Evaluation of PCR and PCR-RFLP protocols to identify the Shiga toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0026/MQ51107.pdf.

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Hill, Philip John. "PCR based gene engineering." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317040.

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Costa, Luciana Fachini da [UNESP]. "Avaliação comparativa entre PCR gênero-específica, PCR espécie-específica e nested PCR espécie-específica no diagnóstico da infecção por Brucella ovis." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/94670.

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Made available in DSpace on 2014-06-11T19:27:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-06-29Bitstream added on 2014-06-13T20:08:22Z : No. of bitstreams: 1 costa_lf_me_botfmvz.pdf: 594547 bytes, checksum: 7d609573704d70bd41e6633e86629767 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>O diagnóstico da infecção por Brucella ovis em ovinos usualmente é realizado por meio de exame clínico, testes sorológicos e bacteriologia. Devido às limitações apresentadas pelas técnicas, o diagnós
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Costa, Luciana Fachini da. "Avaliação comparativa entre PCR gênero-específica, PCR espécie-específica e nested PCR espécie-específica no diagnóstico da infecção por Brucella ovis /." Botucatu : [s.n.], 2010. http://hdl.handle.net/11449/94670.

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Resumo: O diagnóstico da infecção por Brucella ovis em ovinos usualmente é realizado por meio de exame clínico, testes sorológicos e bacteriologia. Devido às limitações apresentadas pelas técnicas, o diagnóstico é geralmente obtido mediante aplicação de duas ou mais técnicas para obtenção de um resultado conclusivo. A Reação em Cadeia pela Polimerase (PCR) tem sido utilizada como ferramenta diagnóstica aplicável, pois é sensível, pouco dispendiosa, rápida, simples de ser realizada e permite o diagnóstico específico do agente infectante. Neste trabalho foram realizados dois experimentos. No pri
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Barker, S. "Planktonic foraminiferal proxies for temperature and pCO2." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596364.

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The thesis aims to describe developments in the uses of planktonic foraminiferal calcite for oceanographic applications, specifically Mg/Ca ratios and foraminiferal shell weights as proxies for calcification temperature and paleo-<i>p</i>CO<sub>2</sub> respectively. Sample preparation for the analysis of Mg/Ca and Sr/Ca ratios in foraminiferal calcite is investigated with the aim of defining a method that will give reproducible results and minimise signal contaminations. Each step of the cleaning procedure is scrutinised in order to gauge which are most important and which may be omitted as un
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Barry, Victoria A. "Calcium signalling and secretion in PC12 cells." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319994.

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Jakobsson, Sanna. "Quantitative analysis of BCR-ABL1 fusion gene by Droplet Digital PCR and qRT-PCR." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-103957.

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Escriva, Michel. "Etude comparée des effets de l'alimentation entérale sur les valeurs du pHi et du gradient tonométrique de PCO2 ([delta] PCO2)." Montpellier 1, 1997. http://www.theses.fr/1997MON11154.

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Sridhar, Srikala. "Molecular regulation of neuronal apoptosis in PC12 cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22018.pdf.

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Mudumba, Sreenivasu. "Characterization of polyamine-induced differentiation in PC12 cells." Scholarly Commons, 1997. https://scholarlycommons.pacific.edu/uop_etds/2609.

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The present investigation focused on three different aspects of polyamine involvement in induction of cell differentiation and neurite outgrowths: effects of polyamines on isolated bovine brain tubulin assembly, effects of polyamines on dopamine levels in PC12 cells, and influence of differentiating agents on polyamine and acetylpolyamine levels in PC12 cells. Among the polyamines, only spermine significantly induced tubulin assembly starting at 60 $\mu$M concentration (p $<$ 0.05). Colchicine at 100 $\mu$M concentration, and calcium chloride at 5 mM concentration inhibited spermine-induced po
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Offermann, Barbara [Verfasser], and Melanie [Akademischer Betreuer] Börries. "Transcriptome dynamics to unravel PC12 cell fate decisions." Freiburg : Universität, 2017. http://d-nb.info/1134967535/34.

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Saukkoriipi, A. (Annika). "Detection of pneumococcus by PCR." Doctoral thesis, University of Oulu, 2003. http://urn.fi/urn:isbn:9514272110.

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Abstract New rapid methods for sensitive and specific detection of pneumococci are not only needed to improve the diagnosis of pneumococcal disease but are also essential for vaccine and carriage studies. The purpose of this study was to develop sensitive PCR methods for the detection and quantification of S. pneumoniae and to study the applicability of these methods to detecting pneumococci in clinical samples. A previously described PCR method was first developed further by introducing a Europium-labelled hybridisation probe for the detection of amplification products. The hybridisation me
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Metivier, Romain. "Ecologie microbienne de produits végétaux : Adaptation de traitements assainissants pour la valorisation de ces produits." Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0419/document.

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L’utilisation de coproduits en tant que matière première provenant d’une autre voie industrielle, fait qu’il n’est plus considéré comme « déchet ». Leur valorisation est donc un axe de développement pour les entreprises agronomiques et agroalimentaires. Cependant, leur nouveau statut de « matière première » entraîne des contraintes pour les industriels.Celles-ci sont diverses selon les voies de destination : sanitaires, toxicologiques …Ce travail s’intéresse à deux coproduits issus de filières différentes de transformation végétale :(1) l’épiderme de pomme, comme source d’antioxydants. Leur va
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Beauchamps, Patrick. "Contribution de l'amplification génique (PCR) au diagnostic de la toxoplasmose : intérêts de la PCR quantitative." Lille 1, 1999. https://pepite-depot.univ-lille.fr/LIBRE/Th_Num/1999/50376-1999-413.pdf.

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La toxoplasmose est une parasitose ubiquitaire occupant une large place en médecine humaine et vétérinaire. Cette affection parasitaire très fréquente en France est le plus souvent bénigne voire asymptomatique. Pourtant, elle reste redoutable en situation de transmission congénitale. De même, sa survenue chez les malades immunodéprimés a radicalement changé la conception de cette maladie. Pour compenser les insuffisances du sérodiagnostic et du diagnostic parasitologique, nous avons mis au point un diagnostic permettant un dépistage très précoce face à la suspicion d'une toxoplasmose congénita
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Ceia, Joana Filipa Simões. "Avaliação do efeito de dois protocolos vacinais para PCV2 em leitões." Master's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2012. http://hdl.handle.net/10400.5/4856.

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Dissertação de Mestrado Integrado em Medicina Veterinária<br>As vacinas comerciais contra o circovírus porcino tipo 2 (PCV2) reduzem significativamente as perdas associadas a este vírus e melhoram os parâmetros produtivos dos efectivos suínos. A maioria dos produtores vacina os leitões ao desmame mas, sendo este um momento crítico da produção, a alteração do momento de vacinação poderá ser uma estratégia a adoptar quando os indivíduos são expostos a múltiplos agentes perturbadores da homeostasia num curto período de tempo. Este estudo teve como objectivo a avaliação do momento mais adequado à
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Chandramoulee, Swaran Yuvaneswari. "Evaluation of direct PCR for forensic DNA profiling and the development of a direct PCR multiplex." Thesis, University of Strathclyde, 2012. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=18935.

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The use of direct PCR with different types of sample was explored in this study. Genomic DNA preparations at various concentrations and buccal cell counts were deposited on commonly encountered substrates, recovered and amplified using direct PCR before subjecting them to capillary electrophoresis. The electropherograms obtained were compared to those obtained using the standard DNA profiling protocol which involves extraction and amplification prior to capillary electrophoresis. Direct PCR was found to be better than the standard DNA profiling protocol in both studies and was further tested w
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Arslan, Giulia. "Adenosine A₂A and ATP receptors in PC12 cells /." Stockholm, 2000. http://diss.kib.ki.se/2000/91-628-4028-2/.

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Chan, Jennifer. "Nicotinic alpha-bungarotoxin receptors in PC12 and H69 cells." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22723.

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Current evidence indicates that there are two populations of nicotinic receptors, the $ alpha$-BGT sensitive and $ alpha$-BGT insensitive neuronal nicotinic receptors. The latter are known to be involved in neurotransmission. However, the physiological significance of the $ alpha$-toxin sensitive population is unclear, although an involvement in trophic functions has been suggested.<br>The present studies were carried out to investigate the possible involvement of the nicotinic $ alpha$-BGT receptor in growth related activity in neuronal PC12 cells and in H69 cells, a small cell lung carcinoma
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Habicher, Marit [Verfasser]. "Zentralvenös – arterielle pCO2 - Differenz bei kardiochirurgischen Patienten / Marit Habicher." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2014. http://d-nb.info/1046563688/34.

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Mandela, Prashant, and Gregory A. Ordway. "KCl Stimulation Increases Norepinephrine Transporter Function in PC12 Cells." Digital Commons @ East Tennessee State University, 2006. https://dc.etsu.edu/etsu-works/8616.

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The norepinephrine transporter (NET) plays a pivotal role in terminating noradrenergic signaling and conserving norepinephrine (NE) through the process of re-uptake. Recent evidence suggests a close association between NE release and regulation of NET function. The present study evaluated the relationship between release and uptake, and the cellular mechanisms that govern these processes. KCl stimulation of PC12 cells robustly increased [ H]NE uptake via the NET and simultaneously increased [ H]NE release. KCl-stimulated increases in uptake and release were dependent on Ca . Treatment of cell
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Tully, Keith. "Channel Specific Calcium Dynamics in PC12 Cells: A Dissertation." eScholarship@UMMS, 2004. https://escholarship.umassmed.edu/gsbs_diss/102.

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Calcium ions (Ca2+) are involved in almost all neuronal functions, providing the link between electrical signals and cellular activity. This work examines the mechanisms by which a neuron can regulate the movement and sequestration of Ca2+ through specific channels such that this ubiquitous ion can encode specific functions. My initial focus was using intracellular calcium ([Ca2+]i) imaging techniques to study the influence of the inhibition of specific voltage gated calcium channels (VGCC) by ethanol on a depolarization induced rise in [Ca2+]i in neurohypophysial nerve terminals. This researc
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Cloux, Boccoz Stéphanie. "Développement de PCRs multiplexes pour le diagnostic : microarrays analytiques." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10282/document.

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Les travaux présentés dans cette thèse font suite à celle de Melle LE GOFF. Ils se concentrent sur la technologie HIFI brevetée et développée pendant ses travaux. Une première partie du travail présenté dans ce manuscrit concerne le test HIFI Blood 96™ et plus particulièrement les améliorations et les évolutions apportées au test afin d'en faire un véritable outil de génotypage, multiparamétrique et haut-débit pouvant être installé dans les banques de sang dans le but de constituer des inventaires de sang génotypé de façon étendue, participant ainsi à améliorer la sécurité transfusionnelle. Il
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Fusaro, Laura <1981&gt. "Patologie da Porcine Circovirus tipo 2 (PCV2) nel suino." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/3640/1/fusaro_laura_tesi.pdf.

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The main work involved the PMWS (Post-weaning multisystemic Wasting Syndrome), caused by PCV-2 (Porcine Circovirus type 2) that involved post-weaned pigs. Merial Italy has funded a study activity in which groups of 3-5 animals were sampled for lungs, tracheo-bronchial and superficial inguinal lymph nodes, ileum and tonsils. The protocol applied can be identified as a more diagnostic potential on the individual than on the group. PNP. Another investigation has been conducted to study proliferative and necrotizing pneumonia (PNP), a form of interstitial pneumonia in weaning and post-weaning pig
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Fusaro, Laura <1981&gt. "Patologie da Porcine Circovirus tipo 2 (PCV2) nel suino." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/3640/.

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The main work involved the PMWS (Post-weaning multisystemic Wasting Syndrome), caused by PCV-2 (Porcine Circovirus type 2) that involved post-weaned pigs. Merial Italy has funded a study activity in which groups of 3-5 animals were sampled for lungs, tracheo-bronchial and superficial inguinal lymph nodes, ileum and tonsils. The protocol applied can be identified as a more diagnostic potential on the individual than on the group. PNP. Another investigation has been conducted to study proliferative and necrotizing pneumonia (PNP), a form of interstitial pneumonia in weaning and post-weaning pig
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39

Bischoff, Erik. "Schnellnachweis von bierschädlichen Bakterien mit PCR." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964420333.

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Lien, Tonje Gulbrandsen. "Statistical Analysis of Quantitative PCR Data." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for matematiske fag, 2011. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-13094.

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This thesis seeks to develop a better understanding of the analysis of gene expression to find the amount of transcript in a sample. The mainstream method used is called Polymerase Chain Reaction (PCR) and it exploits the DNA's ability to replicate. The comparative CT method estimate the starting fluorescence level f0 by assuming constant amplification in each PCR cycle, and it uses the fluorescence level which has risen above a certain threshold. We present a generalization of this method, where different threshold values can be used. The main aim of this thesis is to evaluate a new method ca
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Karlsson, Magdalena, and Emilia Semberg. "Tracing probiotics in salami using PCR." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-157177.

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Starter cultures of different bacteria strains like lactic acid producing bacteria, Staphylococcus and Kocuria are used when making salami. Starter cultures give the sausage specific flavours and improve the quality and ripening of the final product. Probiotic strains can also be added during the production of salami. Studies have shown that probiotics are good for health and are therefore added to food, such as fermented sausages. In order to work as a probiotic strain, the bacteria have to survive during the production process, storage and through the whole human gastrointestinal tract. The
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42

Halpern, Micah. "Immuno-PCR detection of Lyme borreliosis." Doctoral diss., University of Central Florida, 2013. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/6286.

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Lyme borreliosis, more commonly referred to as Lyme disease, is the fastest growing zoonotic disease in North America with approximately 30,000 confirmed cases and 300,000 estimated infections per year. In nature, the causative agent of Lyme disease, the bacterium Borrelia burgdorferi, cycles between Ixodes sp. ticks and small mammals. Humans become infected with Lyme disease after being bitten by an infected tick. The primary indicator of a Borrelia burgdorferi infection is a bull's eye rash typically followed by flu-like symptoms with treatment consisting of a 2-4 week course of antibiotics.
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43

Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.

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Pelikánová, Veronika. "Automatická genotypizace bakterií metodou rep-PCR." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-378029.

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This thesis deals with automatic bacteria genotyping by rep-PCR method. Its theoretical part presents various methods of DNA typing, basic information on electrophoresis and modern electrophoretic approaches, including their problems, misleading data distortion. In order to automate typing, there has been introduced a program for phylogenetic sample classification from rep-PCR, also applicable for data from chip capillary electrophoresis. The program consists of three main parts: digitization, bandmatching and clustering apparatus to bacterial type classification. The result of the algorithm i
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Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.

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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after rep
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Ritterbusch, Giseli Aparecida. "Estudo da patogenicidade e investigação de coinfecção por circovirus suíno e torque teno vírus suíno em material proveniente de porcas com patologias reprodutivas." Universidade do Estado de Santa Catarina, 2009. http://tede.udesc.br/handle/handle/822.

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Made available in DSpace on 2016-12-08T16:24:07Z (GMT). No. of bitstreams: 1 PGCA09MA052.pdf: 726948 bytes, checksum: 2a141d4d92b1c5ac552655f7c9ad3c1a (MD5) Previous issue date: 2009-11-06<br>Many infectious agents have been associated with reproductive failure in swine, representing significantly economic losses for production. Recently, Porcine Circovirus Type 2 (PCV2), etiologic agent of PCVAD or PCV2 associated diseases, was associated with reproductive failure in swine around the world. To confirm the pathogenic potential of PCV2 inducing reproductive failure in sows, it s necessary the
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Guo, Ning. "SIP30 (ZWINT1), a placental mammal specific gene, modulates stimulated vesicle exocytosis and neuropathic pain." Cincinnati, Ohio : University of Cincinnati, 2009. http://www.ohiolink.edu/etd/view.cgi?acc_num=ucin1236015122.

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Thesis (Ph.D.)--University of Cincinnati, 2009.<br>Advisors: Lei Yu PhD (Committee Chair), Anil Menon PhD (Committee Member), James Herman PhD (Committee Member), Robert Brackenbury PhD (Committee Member), Linda Parysek PhD (Committee Member). Title from electronic thesis title page (viewed April 26, 2009). Includes abstract. Includes bibliographical references.
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Reis, Levi Eduardo Soares. "Detecção de Leishmania por PCR e suas variações (seminested PCR e PCR em tempo real), em fragmentos de pele e de baço de cães com leishmaniose visceral." Programa de Pós-Graduação em Ciências Farmacêuticas. CIPHARMA, Escola de Farmácia, Universidade Federal de Ouro Preto, 2013. http://www.repositorio.ufop.br/handle/123456789/3364.

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Submitted by Oliveira Flávia (flavia@sisbin.ufop.br) on 2013-10-10T16:05:16Z No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) DISSERTAÇÃO_DetecçãoLeishmaniaPCR.pdf: 1009268 bytes, checksum: f3643332a0889fcb5d973bed29de784f (MD5)<br>Approved for entry into archive by Gracilene Carvalho (gracilene@sisbin.ufop.br) on 2013-10-21T14:45:17Z (GMT) No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) DISSERTAÇÃO_DetecçãoLeishmaniaPCR.pdf: 1009268 bytes, checksum: f3643332a0889fcb5d973bed29de784f (MD5)<br>Made av
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Noll, Lance. "Escherichia coli O157: detection and quantification in cattle feces by quantitative PCR, conventional PCR, and culture methods." Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/18923.

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Master of Science<br>Department of Diagnostic Medicine/Pathobiology<br>T. G. Nagaraja<br>Shiga toxin-producing E. coli O157 is a major foodborne pathogen. The organism colonizes the hindgut of cattle and is shed in the feces, which serves as a source of contamination of food. Generally, cattle shed E. coli O157 at low concentrations (≤ 10[superscript]2 CFU/g), but a subset of cattle, known as “super-shedders”, shed high concentrations (>10[superscript]3 CFU/g) and are responsible for increased transmission between animals and subsequent hide and carcass contamination. Therefore, concentrati
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Lima, Ana Carolina Stocco de. "Diagnóstico molecular de Leishmaniose Tegumentar Americana: identificação de espécies de Leishmania por SSUrDNA PCR e G6PD PCR." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-20092010-162811/.

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A Leishmaniose Tegumentar Americana (LTA) representa um sério problema de saúde pública. No Brasil muitas espécies são reconhecidas como patogênicas para o homem, portanto o diagnóstico diferencial é necessário para compreender o perfil epimemiológico da LTA em áreas endêmicas. Com o objetivo de identificar espécies de Leishmania utilizando ferramentas moleculares, cinquenta e três biópsias de pele de pacientes com LTA, fixadas em formalina e incluídas em parafina dos Estados do Pará (N=33) e Maranhão (20) foram submetidos a diferentes protocolos da Reação em Cadeia da Polimerase (PCR) para a
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