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Journal articles on the topic 'PcrA'

Author: Grafiati

Published: 4 June 2021

Last updated: 30 January 2023

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1

Bender, Kelly S., Ching Shang, Romy Chakraborty, Sara M. Belchik, John D. Coates, and Laurie A. Achenbach. "Identification, Characterization, and Classification of Genes Encoding Perchlorate Reductase." Journal of Bacteriology 187, no. 15 (2005): 5090–96. http://dx.doi.org/10.1128/jb.187.15.5090-5096.2005.

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ABSTRACT The reduction of perchlorate to chlorite, the first enzymatic step in the bacterial reduction of perchlorate, is catalyzed by perchlorate reductase. The genes encoding perchlorate reductase (pcrABCD) in two Dechloromonas species were characterized. Sequence analysis of the pcrAB gene products revealed similarity to α- and β-subunits of microbial nitrate reductase, selenate reductase, dimethyl sulfide dehydrogenase, ethylbenzene dehydrogenase, and chlorate reductase, all of which are type II members of the microbial dimethyl sulfoxide (DMSO) reductase family. The pcrC gene product was

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2

Gupta, Nidhi, and A. K. Srivastava. "Interpenetrating Polymer Networks Based on Poly Chromium Acrylate/Poly Acrylonitrile: Synthesis and Properties of Semi IPN-1." High Performance Polymers 4, no. 4 (1992): 225–35. http://dx.doi.org/10.1088/0954-0083/4/4/003.

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A series of semi-I tpe interpenetrating polymer networks (IPN) based on poly chromium acrylate and poly acrylonitrile crosslinked with divinyl benzene have been synthesized. Synthetic details, including concentration of poly chromium acriylate (PCrA), acrylonitrile (AN) and divinyl benzene (DVB) and average molecular weight of PCrA were varied and their effect on the crosslink density of the network was studied by swelling experiments. High [PCrAJ and low [AN] increases swelling and thereby average molecular weight between crosslinks (M,). SEM micrographs and glass transition temperature show

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3

Yang, Hongjing, Zhiying Shan, Jaewha Kim, et al. "Regulatory Role of PopN and Its Interacting Partners in Type III Secretion of Pseudomonas aeruginosa." Journal of Bacteriology 189, no. 7 (2007): 2599–609. http://dx.doi.org/10.1128/jb.01680-06.

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ABSTRACT The type III secretion system (T3SS) of Pseudomonas aeruginosa plays a significant role in pathogenesis. We have previously identified type III secretion factor (TSF), which is required for effective secretion of the type III effector molecules, in addition to the low calcium signal. TSF includes many low-affinity high-capacity calcium binding proteins, such as serum albumin and casein. A search for the TSF binding targets on the bacterial outer membrane resulted in identification of PopN, a component of the T3SS that is readily detectable on the bacterial cell surface. PopN specifica

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4

Anand, Syam P., Haocheng Zheng, Piero R. Bianco, Sanford H. Leuba, and Saleem A. Khan. "DNA Helicase Activity of PcrA Is Not Required for the Displacement of RecA Protein from DNA or Inhibition of RecA-Mediated Strand Exchange." Journal of Bacteriology 189, no. 12 (2007): 4502–9. http://dx.doi.org/10.1128/jb.00376-07.

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ABSTRACT PcrA is a conserved DNA helicase present in all gram-positive bacteria. Bacteria lacking PcrA show high levels of recombination. Lethality induced by PcrA depletion can be overcome by suppressor mutations in the recombination genes recFOR. RecFOR proteins load RecA onto single-stranded DNA during recombination. Here we test whether an essential function of PcrA is to interfere with RecA-mediated DNA recombination in vitro. We demonstrate that PcrA can inhibit the RecA-mediated DNA strand exchange reaction in vitro. Furthermore, PcrA displaced RecA from RecA nucleoprotein filaments. In

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5

Ruiz-Masó, J. A., S. P. Anand, M. Espinosa, S. A. Khan, and G. del Solar. "Genetic and Biochemical Characterization of the Streptococcus pneumoniae PcrA Helicase and Its Role in Plasmid Rolling Circle Replication." Journal of Bacteriology 188, no. 21 (2006): 7416–25. http://dx.doi.org/10.1128/jb.01010-06.

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ABSTRACT PcrA is a chromosomally encoded DNA helicase of gram-positive bacteria involved in replication of rolling circle replicating plasmids. Efficient interaction between PcrA and the plasmid-encoded replication initiator (Rep) protein is considered a requirement for the plasmid to replicate in a given host, and thus, the ability of a Rep protein to interact with heterologous PcrA helicases has been invoked as a determinant of plasmid promiscuity. We characterized transcription of the Streptococcus pneumoniae pcrA gene in its genetic context and studied the biochemical properties of its pro

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6

Trivella, Maria Giovanna, Alessandra Piersigilli, Fabio Bernini, et al. "Percutaneous Cardiac Support during Myocardial Infarction Drastically Reduces Mortality: Perspectives from a Swine Model." International Journal of Artificial Organs 40, no. 7 (2017): 338–44. http://dx.doi.org/10.5301/ijao.5000604.

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Background/Aims Acute myocardial infarction (AMI) with cardiogenic shock (CS) remains the leading cause of in-hospital death in acute coronary syndromes. In the AMI-CS pig model we tested the efficacy of temporary percutaneous cardiorespiratory assist device (PCRA) in rescuing the failing heart and reducing early mortality. Methods In open-chest pigs we induced AMI by proximal left anterior descending coronary artery (LAD) ligation. Eight animals without PCRA (C group) were compared with 12 animals otherwise treated with PCRA (T group), starting approximately at 60 minutes post-occlusion and l

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7

Moreno-del Álamo, María, Begoña Carrasco, Rubén Torres, and Juan Carlos Alonso. "Bacillus subtilis PcrA Helicase Removes Trafficking Barriers." Cells 10, no. 4 (2021): 935. http://dx.doi.org/10.3390/cells10040935.

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Bacillus subtilis PcrA interacts with the RNA polymerase and might contribute to mitigate replication–transcription conflicts (RTCs). We show that PcrA depletion lethality is partially suppressed by rnhB inactivation, but cell viability is significantly reduced by rnhC or dinG inactivation. Following PcrA depletion, cells lacking RnhC or DinG are extremely sensitive to DNA damage. Chromosome segregation is not further impaired by rnhB or dinG inactivation but is blocked by rnhC or recA inactivation upon PcrA depletion. Despite our efforts, we could not construct a ΔrnhC ΔrecA strain. These obs

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8

Naqvi, Asma, Eowyn Tinsley, and Saleem A. Khan. "Purification and Characterization of the PcrA Helicase of Bacillus anthracis." Journal of Bacteriology 185, no. 22 (2003): 6633–39. http://dx.doi.org/10.1128/jb.185.22.6633-6639.2003.

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ABSTRACT PcrA is an essential helicase in gram-positive bacteria, and a gene encoding this helicase has been identified in all such organisms whose genomes have been sequenced so far. The precise role of PcrA that makes it essential for cell growth is not known; however, PcrA does not appear to be necessary for chromosome replication. The pcrA gene was identified in the genome of Bacillus anthracis on the basis of its sequence homology to the corresponding genes of Bacillus subtilis and Staphylococcus aureus, with which it shares 76 and 72% similarity, respectively. The pcrA gene of B. anthrac

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9

Nozawa-Inoue, Mamie, Mercy Jien, Nicholas S. Hamilton, Valley Stewart, Kate M. Scow, and Krassimira R. Hristova. "Quantitative Detection of Perchlorate-Reducing Bacteria by Real-Time PCR Targeting the Perchlorate Reductase Gene." Applied and Environmental Microbiology 74, no. 6 (2008): 1941–44. http://dx.doi.org/10.1128/aem.01658-07.

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ABSTRACT A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

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10

Carrasco, Carolina, Cesar L. Pastrana, Clara Aicart-Ramos, Sanford H. Leuba, Saleem A. Khan, and Fernando Moreno-Herrero. "Dynamics of DNA nicking and unwinding by the RepC–PcrA complex." Nucleic Acids Research 48, no. 4 (2020): 2013–25. http://dx.doi.org/10.1093/nar/gkz1200.

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Abstract The rolling-circle replication is the most common mechanism for the replication of small plasmids carrying antibiotic resistance genes in Gram-positive bacteria. It is initiated by the binding and nicking of double-stranded origin of replication by a replication initiator protein (Rep). Duplex unwinding is then performed by the PcrA helicase, whose processivity is critically promoted by its interaction with Rep. How Rep and PcrA proteins interact to nick and unwind the duplex is not fully understood. Here, we have used magnetic tweezers to monitor PcrA helicase unwinding and its relat

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11

Chen, Si, Zhonghuang Xu, Hongju Liu, et al. "Perioperative patient-controlled regional analgesia versus patient-controlled intravenous analgesia for patients with critical limb ischaemia: a study protocol for a randomised controlled trial." BMJ Open 10, no. 10 (2020): e037879. http://dx.doi.org/10.1136/bmjopen-2020-037879.

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IntroductionBoth regional analgesia and intravenous analgesia are frequently used perioperatively for patients with critical limb ischaemia (CLI). Nevertheless, the comparison of perioperative effect of regional and intravenous analgesia has not yet been thoroughly illustrated. This study will comprehensively compare patient-controlled regional analgesia (PCRA) and patient-controlled intravenous analgesia (PCIA) as two different perioperative analgesia approaches for patients with CLI. It investigates their effects on analgesia, reperfusion and the quality of recovery perioperatively, also aim

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12

Lowenkamp, Christopher T., Alexander M. Holsinger, and Thomas H. Cohen. "PCRA revisited: Testing the validity of the Federal Post Conviction Risk Assessment (PCRA)." Psychological Services 12, no. 2 (2015): 149–57. http://dx.doi.org/10.1037/ser0000024.

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13

Jiménez, A. P., L. Z. Duarte, L. S. Cortez, et al. "Associação à positividade da Lawsonia intracellularis com a expressão clínico-patológica da infecção em suínos da região metropolitana de Bucaramanga (Santander, Colômbia)." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70, no. 1 (2018): 181–86. http://dx.doi.org/10.1590/1678-4162-9182.

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RESUMO Porções de íleo terminal foram coletados de 100 suínos com sinais de doença gastrointestinal na área metropolitana de Bucaramanga, a fim de se estudar a eficiência do diagnóstico de enteropatia proliferativa suína (PPE) pela técnica de PCR aninha (PCRa) empregando sequências específicas (primers) para L. intracellularis: 16S ARN região (270pb) e sua correlação com achados clínicos e patológicos. Todas as amostras foram processadas para se determinar a associação entre positividade por PCR, os sinais clínicos, os achados de necropsia e as lesões histológicas. Cinquenta e seis por cento d

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14

Anand, Syam P., Poulami Mitra, Asma Naqvi, and Saleem A. Khan. "Bacillus anthracis and Bacillus cereus PcrA Helicases Can Support DNA Unwinding and In Vitro Rolling-Circle Replication of Plasmid pT181 of Staphylococcus aureus." Journal of Bacteriology 186, no. 7 (2004): 2195–99. http://dx.doi.org/10.1128/jb.186.7.2195-2199.2004.

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ABSTRACT Replication of rolling-circle replicating (RCR) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid DNA by the PcrA helicase. In this report, we demonstrate that heterologous PcrA helicases from Bacillus anthracis and Bacillus cereus are capable of unwinding Staphylococcus aureus plasmid pT181 from the initiator-generated nick and promoting in vitro replication of the plasmid. These helicases also physically interact with the RepC initiator protein of pT181. The ability of PcrA helicases to unwind noncognate RCR plasmids may contribute to the

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15

Tufiño-Loza, Catalina, José Juan Martínez-Maya, Amaury Carrillo-González, et al. "Uso de una PCR anidada para el diagnóstico del virus de la necrosis pancreática infecciosa (VNPI) en truchas de campo." Revista Mexicana de Ciencias Pecuarias 11, no. 3 (2020): 811–27. http://dx.doi.org/10.22319/rmcp.v11i3.5242.

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El aislamiento en cultivo celular es el método principal para el diagnóstico del virus de la necrosis pancreática infecciosa (VNPI). Aunque es un método confiable, resulta costoso y conlleva tres semanas en proporcionar un resultado. Para disminuir el tiempo en el diagnóstico y aumentar la sensibilidad para la detección del VNPI, el objetivo del presente estudio fue establecer y evaluar el uso de una PCR anidada (PCRa) para un diagnóstico rápido del VNPI. Para ello, se diseñaron dos pares de iniciadores basados en secuencias mexicanas. El primer par (RT-PCR) amplificó un producto de 682 pb y e

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16

Rawal, Narinder, Renée Allvin, Kjell Axelsson, et al. "Patient-controlled Regional Analgesia (PCRA) at Home." Anesthesiology 96, no. 6 (2002): 1290–96. http://dx.doi.org/10.1097/00000542-200206000-00005.

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Background The aim of this randomized, double-blinded study was to compare the analgesic efficacy of bupivacaine versus ropivacaine brachial plexus analgesia after ambulatory hand surgery. An additional aim was to study the feasibility and safety of patient-controlled regional analgesia (PCRA) outside the hospital. Methods Sixty patients scheduled for ambulatory hand surgery underwent surgery with axillary plexus blockade. After surgery, the plexus catheter was connected to an elastomeric, disposable "homepump," containing 100 ml of either 0.125% bupivacaine or 0.125% ropivacaine. When patient

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17

Thickman, Karen R., Sanford H. Leuba, and Saleem A. Khan. "DNA Structure Specificity of Bacillus Stearothermophilus PcrA." Biophysical Journal 98, no. 3 (2010): 73a. http://dx.doi.org/10.1016/j.bpj.2009.12.415.

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18

Yun, Sangwon, Yumin Jo, Seojin Sim, et al. "Comparison of continuous and single interscalene block for quality of recovery score following arthroscopic rotator cuff repair." Journal of Orthopaedic Surgery 29, no. 1 (2021): 230949902110001. http://dx.doi.org/10.1177/23094990211000142.

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Background:Continuous interscalene brachial plexus block (CISB) is well known to reduce postoperative pain and to improve patient satisfaction. However, the effect of CISB on the quality of postoperative recovery is unknown. We Compared the quality of recovery from arthroscopic rotator cuff repair in patients who received CISB or single interscalene brachial plexus block (SISB).Methods:This prospective non-randomized controlled trial with propensity score matching enrolled 134 patients undergoing arthroscopic surgery for rotator cuff repair. Each patient received an interscalene block before s

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19

DeLisi, Matt, Alan Drury, and Michael Elbert. "Who Are the Compliant Correctional Clients? New Evidence on Protective Factors among Federal Supervised Releases." International Journal of Offender Therapy and Comparative Criminology 65, no. 13-14 (2021): 1536–53. http://dx.doi.org/10.1177/0306624x21992681.

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Protective factors facilitate success on community supervision, but relatively little is known about correctional clients who are highly compliant particularly in the federal system. Drawing on a near population of federal clients on supervised release in the Midwestern United States, the current study examined variables associated with compliant supervision status. One day on supervision contributed to a 1% reduction in the logged odds of supervision compliance. Clients with no drug history had 793% increased odds, clients with sustained remission had 620% increased odds, and clients with ear

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20

Dittrich, Markus, and Klaus Schulten. "PcrA Helicase, a Prototype ATP-Driven Molecular Motor." Structure 14, no. 9 (2006): 1345–53. http://dx.doi.org/10.1016/j.str.2006.06.017.

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21

Toseland, Christopher P., and Martin R. Webb. "PcrA Helicase ATPase Mechanism: RepD Modulation During Unwinding." Biophysical Journal 96, no. 3 (2009): 415a. http://dx.doi.org/10.1016/j.bpj.2008.12.2122.

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22

Chène, Patrick. "PcrA/UvrD/Rep DNA helicases in bacterial genomes." Biochemical Systematics and Ecology 36, no. 2 (2008): 101–9. http://dx.doi.org/10.1016/j.bse.2007.08.001.

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23

Toseland, Christopher P., Christopher Batters, and Claudia Veigel. "Single Molecule Fluorescence and Force Measurements on PcrA Helicase." Biophysical Journal 100, no. 3 (2011): 72a—73a. http://dx.doi.org/10.1016/j.bpj.2010.12.598.

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24

Delagoutte, Emmanuelle, and Peter H. von Hippel. "Helicase mechanisms and the coupling of helicases within macromolecular machines Part I: Structures and properties of isolated helicases." Quarterly Reviews of Biophysics 35, no. 4 (2002): 431–78. http://dx.doi.org/10.1017/s0033583502003852.

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1. Mechanisms of nucleic acid (NA) unwinding by helicases 4322. Helicases may take advantage of ‘breathing’ fluctuations in dsNAs 4342.1 Stability and dynamics of dsNAs 4342.2 dsNAs ‘breathe’ in isolation 4352.3 Thermodynamics of terminal base pairs of dsNA 4382.4 Thermal fluctuations may be responsible for sequential base-pair opening at replication forks 4392.5 Helicases may capture single base-pair opening events sequentially 4403. Biochemical properties of helicases 4433.1 Binding of NAs 4433.2 Binding and hydrolysis of NTP 4453.3 Coordination between NA binding and NTP binding and hydroly

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25

Yu, Jin, Taekjip Ha, and Klaus Schulten. "Structure-Based Model of the Stepping Motor of PcrA Helicase." Biophysical Journal 91, no. 6 (2006): 2097–114. http://dx.doi.org/10.1529/biophysj.106.088203.

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26

Bertram, Richard D., Christopher J. Hayes, and Panos Soultanas. "Vinylphosphonate Internucleotide Linkages Inhibit the Activity of PcrA DNA Helicase." Biochemistry 41, no. 24 (2002): 7725–31. http://dx.doi.org/10.1021/bi025755s.

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27

Anand, S. P. "Structure-specific DNA binding and bipolar helicase activities of PcrA." Nucleic Acids Research 32, no. 10 (2004): 3190–97. http://dx.doi.org/10.1093/nar/gkh641.

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28

Gavrilov, Momcilo, Ram Tippana, Dmitriy Bobrovnikov, and Taekjip Ha. "Nanopore Detects Compromise between Speed and Processivity of PcrA Helicase." Biophysical Journal 116, no. 3 (2019): 76a. http://dx.doi.org/10.1016/j.bpj.2018.11.449.

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29

Axelsson, K., U. Nordenson, E. Johanzon, et al. "Patient-controlled regional analgesia (PCRA) with ropivacaine after arthroscopic subacromial decompression†." Acta Anaesthesiologica Scandinavica 47, no. 8 (2003): 993–1000. http://dx.doi.org/10.1034/j.1399-6576.2003.00146.x.

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30

Southerden, Lesley, Claudia Arbore, and Martin Webb. "PcrA Helicase and the Mechanism of Asymmetric Rolling Circle DNA Replication." Biophysical Journal 106, no. 2 (2014): 72a. http://dx.doi.org/10.1016/j.bpj.2013.11.475.

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31

Hingley, Richard. "Struggling with a Roman inheritance. A response to Versluys." Archaeological Dialogues 21, no. 1 (2014): 20–24. http://dx.doi.org/10.1017/s138020381400004x.

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I am very grateful to Miguel John Versluys for this paper, which raises several important issues that derive from current debates in Roman archaeology. I am aware of the context of Versluys's arguments as I am a contributor to the forthcoming volumeGlobalization and the Roman world(which Versluys has jointly edited; Pitts and Versluys 2014). I am pleased to be able to develop some of the themes outlined in my chapter for that volume (Hingley 2014b) through this reflection upon Versluys's contribution to the developing debate. The issues raised by Versluys are particularly timely since a number

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32

Grelat, Michael, Chang-Zhi Du, Liang Xu, Xu Sun, and Yong Qiu. "Under-contouring of rods: a potential risk factor for proximal junctional kyphosis after posterior correction of Scheuermann kyphosis." Journal of Neurosurgery: Spine 33, no. 6 (2020): 830–37. http://dx.doi.org/10.3171/2020.5.spine20229.

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OBJECTIVEScheuermann kyphosis (SK) could require surgical treatment in certain situations. A posterior reduction is the most widespread treatment so far, although the development of proximal junctional kyphosis (PJK) is one of the possible complications of this procedure. The contour of the proximal part of the rod could influence the occurrence of PJK in SK patients. The objective of this study was to analyze the impact of the proximal rod contour on the occurrence of a PJK complication in SK patients.METHODSThis retrospective monocentric study was performed in the Nanjing Spine Surgery Depar

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33

Bird, L. "Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism." Nucleic Acids Research 26, no. 11 (1998): 2686–93. http://dx.doi.org/10.1093/nar/26.11.2686.

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34

Soultanas, P. "Plasmid replication initiator protein RepD increases the processivity of PcrA DNA helicase." Nucleic Acids Research 27, no. 6 (1999): 1421–28. http://dx.doi.org/10.1093/nar/27.6.1421.

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35

Lowenkamp, Christopher T., James L. Johnson, Alexander M. Holsinger, Scott W. VanBenschoten, and Charles R. Robinson. "The federal Post Conviction Risk Assessment (PCRA): A construction and validation study." Psychological Services 10, no. 1 (2013): 87–96. http://dx.doi.org/10.1037/a0030343.

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36

Wigley, Dale B. "DNA helicases: One small step for PcrA, one giant leap for RecBC?" Current Biology 10, no. 12 (2000): R444—R446. http://dx.doi.org/10.1016/s0960-9822(00)00529-7.

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37

Merrikh, Christopher N., Bonita J. Brewer, and Houra Merrikh. "The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units." PLOS Genetics 11, no. 6 (2015): e1005289. http://dx.doi.org/10.1371/journal.pgen.1005289.

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38

Noirot-Gros, M. F., P. Soultanas, D. Wigley, S. Ehrlich, P. Noirot, and M. A. Petit. "The beta-propeller protein YxaL increases the processivity of the PcrA helicase." Molecular Genetics and Genomics 267, no. 3 (2002): 391–400. http://dx.doi.org/10.1007/s00438-002-0670-9.

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39

Toleikis, Algirdas, Simone Kunzelmann, Gregory I. Mashanov, Martin R. Webb, and Justin E. Molloy. "DNA Unwinding by PcrA Helicase and RepD using TIRF and Magnetic Tweezers." Biophysical Journal 106, no. 2 (2014): 272a. http://dx.doi.org/10.1016/j.bpj.2013.11.1593.

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40

Park, Jeehae, Sua Myong, Anita Niedziela-Majka, et al. "PcrA Helicase Dismantles RecA Filaments by Reeling in DNA in Uniform Steps." Cell 142, no. 4 (2010): 544–55. http://dx.doi.org/10.1016/j.cell.2010.07.016.

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41

Yang, Ye, Shuo-Xing Dou, Hua Ren, et al. "Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding." Nucleic Acids Research 36, no. 6 (2008): 1976–89. http://dx.doi.org/10.1093/nar/gkm1174.

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42

Zhang, Wenke, Mark S. Dillingham, Christopher D. Thomas, Stephanie Allen, Clive J. Roberts, and Panos Soultanas. "Directional Loading and Stimulation of PcrA Helicase by the Replication Initiator Protein RepD." Journal of Molecular Biology 371, no. 2 (2007): 336–48. http://dx.doi.org/10.1016/j.jmb.2007.05.050.

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43

DeLisi, Matt, Michael J. Elbert, and Alan J. Drury. "Federal Criminal Careers: an Empirical Examination of the Post-Conviction Risk Assessment (PCRA)." American Journal of Criminal Justice 43, no. 4 (2018): 792–809. http://dx.doi.org/10.1007/s12103-018-9436-8.

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44

Toseland, Christopher P., Maria M. Martinez-Senac, Andrew F. Slatter, and Martin R. Webb. "The ATPase Cycle of PcrA Helicase and Its Coupling to Translocation on DNA." Journal of Molecular Biology 392, no. 4 (2009): 1020–32. http://dx.doi.org/10.1016/j.jmb.2009.07.071.

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45

Laszlo, Andrew H., Jonathan M. Craig, Henry Brinkerhoff, et al. "Analysis of Force Dependence of Translocation and Unwinding of Helicase PCRA using SPRNT." Biophysical Journal 114, no. 3 (2018): 91a. http://dx.doi.org/10.1016/j.bpj.2017.11.541.

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46

Bröms, Jeanette E., Petra J. Edqvist, Katrin E. Carlsson, Åke Forsberg, and Matthew S. Francis. "Mapping of a YscY Binding Domain within the LcrH Chaperone That Is Required for Regulation of Yersinia Type III Secretion." Journal of Bacteriology 187, no. 22 (2005): 7738–52. http://dx.doi.org/10.1128/jb.187.22.7738-7752.2005.

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ABSTRACT Type III secretion systems are used by many animal and plant interacting bacteria to colonize their host. These systems are often composed of at least 40 genes, making their temporal and spatial regulation very complex. Some type III chaperones of the translocator class are important regulatory molecules, such as the LcrH chaperone of Yersinia pseudotuberculosis. In contrast, the highly homologous PcrH chaperone has no regulatory effect in native Pseudomonas aeruginosa or when produced in Yersinia. In this study, we used LcrH-PcrH chaperone hybrids to identify a discrete region in the

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Alam, Sk Mahasin, Soma Samanta, Amit Kumar Halder, Soumya Basu та Tarun Jha. "Structural finding of R/S-3,4-dihydro-2,2-dimethyl-6-halo-4-(substituted phenylaminocarbonylamino)-2H-1-benzopyrans as selective pancreatic β-cells KATP-pβ channel openers". Canadian Journal of Chemistry 85, № 12 (2007): 1053–63. http://dx.doi.org/10.1139/v07-127.

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R/S-3,4-Dihydro-2,2-dimethyl-6-halo-4-(substituted phenylaminocarbonyl-amino)-2H-1-benzopyrans are pancreatic β-cells potassium (KATP-pβ) channel openers with inhibitory effect on insulin secretion. To find the more active and effective benzopyrans as selective potassium (KATP-pβ) channel openers towards the pancreatic tissues, quantitative structure–activity relationships (QSAR) study was performed using E-state and R-state indices along with Wang–Ford charges, n-octanol/water partition coefficient, molar refractivity, and indicator parameters. QSAR models were developed by statistical techni

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Kee, W. D. Ngan, K. K. Lam, P. P. Chen, and T. Gin. "Comparison of Patient-Controlled Epidural Analgesia with Patient-Controlled Intravenous Analgesia Using Pethidine or Fentanyl." Anaesthesia and Intensive Care 25, no. 2 (1997): 126–32. http://dx.doi.org/10.1177/0310057x9702500203.

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We compared patient-controlled epidural analgesia (PCEA) with patient-controlled intravenous analgesia (PCIA) using pethidine or fentanyl in a randomized, double-blind crossover study of 80 patients after caesarean section. Patients received pethidine by PCEA or PCIA, or fentanyl by PCEA or PCIA, with a crossover of the route of administration at 12h. For pethidine, pain scores were lower with PCEA vs PCIA from 4 to 16 h (P<0.05). Pethidine consumption was lower with PCEA vs PCIA from 12 to 24 h (P=0.0005). Patients preferred PCEA to PCIA (P=0.015). For fentanyl, pain scores were lower with

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Guarino, Francesco, Oriana Motta, Mimmo Turano, Antonio Proto, and Giovanni Vigliotta. "Preferential Use of the Perchlorate over the Nitrate in the Respiratory Processes Mediated by the Bacterium Azospira sp. OGA 24." Water 12, no. 8 (2020): 2220. http://dx.doi.org/10.3390/w12082220.

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Here we report the results obtained for a strain isolated from a polluted site and classified as Azospira sp. OGA 24. The capability of OGA 24 to utilize perchlorate and nitrate and the regulation of pathways were investigated by growth kinetic studies and analysis of messenger RNA (mRNA) expression of the genes of perchlorate reductase alpha subunit (pcrA), chlorite dismutase (cld), and periplasmic nitrate reductase large subunit (napA). In aerobic conditions and in a minimal medium containing 10 mM acetate as carbon source, 5.6 ± 0.34 mmol L−1 perchlorate or 9.7 ± 0.22 mmol L−1 nitrate were

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Dubaele, Sandy, Christophe Martin, Jacqueline Bohn, and Patrick Chene. "Biochemical Study of Recombinant PcrA from Staphylococcus aureus for the Development of Screening Assays." BMB Reports 40, no. 1 (2007): 7–14. http://dx.doi.org/10.5483/bmbrep.2007.40.1.007.

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