Relevant bibliographies by topics / PDNA purification

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters

Academic literature on the topic 'PDNA purification'

Author: Grafiati
Published: 28 May 2022
Last updated: 31 July 2025

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Journal articles on the topic "PDNA purification"

1

Pavlin, Nejc. "PATfix™ pDNA platform for monitoring pDNA purification process." Cell and Gene Therapy Insights 7, no. 10 (2021): 1437. http://dx.doi.org/10.18609/cgti.2021.190.

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Paalme, Viiu, and Mart Speek. "Simple, inexpensive and RNase-free purification of plasmid DNA by fractional precipitation with isopropanol." BioTechniques 71, no. 3 (2021): 490–94. http://dx.doi.org/10.2144/btn-2021-0018.

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We present a modified alkaline lysis method for purification of plasmid DNA (pDNA) from bacterial extract using fractional precipitation with isopropanol (FPI). This method includes two successive precipitations with 0.33 and 0.36 volumes of isopropanol and separates pDNA from total RNA and most of the lipopolysaccharides. Using different quality control tests, we demonstrate that plasmids purified with FPI show superior quality compared to plasmids prepared with commercial kits based on spin-column chromatography.
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da Silva, Nuno R., Paula Jorge, José A. Martins, José A. Teixeira, and João C. Marcos. "Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems." Life 11, no. 11 (2021): 1138. http://dx.doi.org/10.3390/life11111138.

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Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA remains the key manufacturing step. Despite its high resolution, the scaling-up of chromatography is usually difficult and presents low capacity, resulting in low yields. Alternative methods that are based on aqueous two-phase systems (ATPSs) have been
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Santos, Tiago, Patrícia Pereira, Fani Sousa, João A. Queiroz, and Carla Cruz. "Purification of supercoiled G-quadruplex pDNA for in vitro transcription." Separation and Purification Technology 163 (May 2016): 59–71. http://dx.doi.org/10.1016/j.seppur.2016.02.036.

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5

Castilho, Pedro H., Tiago R. Correia, Maria T. Pessoa de Amorim, et al. "Modification of microfiltration membranes by hydrogel impregnation for pDNA purification." Journal of Applied Polymer Science 132, no. 21 (2014): n/a. http://dx.doi.org/10.1002/app.41610.

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Deshmukh, N. R., and A. M. Lali. "Adsorptive purification of pDNA on superporous rigid cross-linked cellulose matrix." Journal of Chromatography B 818, no. 1 (2005): 5–10. http://dx.doi.org/10.1016/j.jchromb.2004.08.025.

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Valente, J. F. A., A. Sousa, J. A. Queiroz, and F. Sousa. "Selective purification of supercoiled p53-encoding pDNA with l-methionine–agarose matrix." Analytical Biochemistry 459 (August 2014): 61–69. http://dx.doi.org/10.1016/j.ab.2014.05.011.

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Valente, J. F. A., A. Sousa, J. A. Queiroz, and F. Sousa. "DoE to improve supercoiled p53-pDNA purification by O-phospho-l-tyrosine chromatography." Journal of Chromatography B 1105 (January 2019): 184–92. http://dx.doi.org/10.1016/j.jchromb.2018.12.002.

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Wiendahl, Matthias, Stefan A. Oelmeier, Florian Dismer, and Jürgen Hubbuch. "High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification." Journal of Separation Science 35, no. 22 (2012): 3197–207. http://dx.doi.org/10.1002/jssc.201200310.

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10

Schmidt, Axel, Heribert Helgers, Florian Lukas Vetter, Alex Juckers, and Jochen Strube. "Digital Twin of mRNA-Based SARS-COVID-19 Vaccine Manufacturing towards Autonomous Operation for Improvements in Speed, Scale, Robustness, Flexibility and Real-Time Release Testing." Processes 9, no. 5 (2021): 748. http://dx.doi.org/10.3390/pr9050748.

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Supplying SARS-COVID-19 vaccines in quantities to meet global demand has a bottleneck in manufacturing capacity. Assessment of existing mRNA (messenger ribonucleic acid) vaccine processing shows the need for digital twins enabled by process analytical technology approaches to improve process transfers for manufacturing capacity multiplication, reduction of out-of-specification batch failures, qualified personnel training for faster validation and efficient operation, optimal utilization of scarce buffers and chemicals, and faster product release. A digital twin of the total pDNA (plasmid deoxy
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Dissertations / Theses on the topic "PDNA purification"

1

Gungunes, H., R. Uzek, L. Uzun, S. Ozkara, S. Senel, and A. Denizli. "Magnetic Nanoparticles for Plasmid DNA Adsorption via Hydrophobic Interaction." Thesis, Sumy State University, 2012. http://essuir.sumdu.edu.ua/handle/123456789/34946.

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This study aims the preparation of magnetic poly(2-hydroxyethylmethacrylate-N-methacryloyl-Lphenylalanine), [poly(HEMA-MAPA)] nanoparticles for plasmid DNA separation on the basis of hydrophobic interactions. Magnetic nanoparticles will be produced emulsion polymerization of 2- hydroxyethylmethacrylate (HEMA) and N-methacryloyl-L-phenylalanine (MAPA) monomers. Nanosized particles including hydrophobic groups stemmed from polymerizable derivative of phenylalanine aminoacid were evaluated to offer surface area that is enough for the higher capacity DNA purification than commercial micronsi
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2

Pires, Carolina Freitas. "Production and purification of pDNA as a biopharmaceutical with application in oncolog." Master's thesis, 2021. http://hdl.handle.net/10773/33026.

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This work reports the development of an upstream and downstream process to recover a model plasmid DNA (pDNA) with potential application in gene therapy, particularly targeting breast cancer. After the pDNA production, aqueous biphasic systems (ABS) were investigated to as a recovery and purification technique. The ABS composed of the tetrabutylphospnium bromide ([P4444]Br) ionic liquid (IL) and potassium citrate salt (C5H6K3O7·H2O) was identified as the most promising system promoting the isolation of pDNA with high purity. The partitioning behaviour of pDNA and ribonucleic acid (RNA) showed
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Gomes, Diana Vanessa Duarte. "Gellan microspheres application for capture or purification of plasmid DNA vaccine." Master's thesis, 2019. http://hdl.handle.net/10400.6/10506.

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Cervical cancer is the 4th cause of death among women worldwide and is profoundly associated with HPV infection, due to apoptosis inhibition and uncontrolled cell proliferation caused by oncoproteins E6 and E7 action. At the moment, some prophylactic vaccines are available in the market, but that are only capable of preventing HPV infection. Thus, the development of effective treatment for HPV-infected individuals is fundamental. DNA vaccines emerged as a promising way to prevent and treat several diseases since it can stimulate both cellular and humoral immune responses. The biotechnological
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Books on the topic "PDNA purification"

1

S, Robertson James, Parenteral Drug Association, and European Agency for the Evaluation of Medicinal Products., eds. PDA/EMEA European Virus Safety Forum: Paul-Ehrlich-Institut, Langen, Germany, September 29 - October 1, 2003 ; proceedings of a conference. Karger, 2004.

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Book chapters on the topic "PDNA purification"

1

Sousa, Ângela, João A. Queiroz, and Fani Sousa. "p53-Encoding pDNA Purification by Affinity Chromatography for Cancer Therapy." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2727-2_7.

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Journal articles
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